Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene017582.t1 | XP_003629214.1 | 91.5 | 378 | 31 | 1 | 1 | 378 | 1 | 377 | 8.20E-209 | 736.1 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene017582.t1 | Q9SHX9 | 30.4 | 358 | 213 | 10 | 6 | 353 | 3 | 334 | 4.8e-32 | 140.2 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene017582.t1 | G7LA99 | 91.5 | 378 | 31 | 1 | 1 | 378 | 1 | 377 | 5.9e-209 | 736.1 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene97013 | MS.gene017582 | PPI |
| MS.gene012160 | MS.gene017582 | PPI |
| MS.gene66307 | MS.gene017582 | PPI |
| MS.gene017582 | MS.gene017581 | PPI |
| MS.gene012159 | MS.gene017582 | PPI |
| MS.gene64423 | MS.gene017582 | PPI |
| MS.gene017582 | MS.gene39445 | PPI |
| MS.gene017582 | MS.gene064059 | PPI |
| MS.gene037197 | MS.gene017582 | PPI |
| MS.gene30094 | MS.gene017582 | PPI |
| MS.gene017582 | MS.gene025209 | PPI |
| MS.gene91760 | MS.gene017582 | PPI |
| MS.gene023531 | MS.gene017582 | PPI |
| MS.gene28903 | MS.gene017582 | PPI |
| MS.gene23497 | MS.gene017582 | PPI |
| MS.gene39445 | MS.gene017582 | PPI |
| MS.gene017582 | MS.gene08770 | PPI |
| MS.gene28904 | MS.gene017582 | PPI |
| MS.gene44191 | MS.gene017582 | PPI |
| MS.gene017582 | MS.gene023531 | PPI |
| MS.gene025209 | MS.gene017582 | PPI |
| MS.gene44190 | MS.gene017582 | PPI |
| MS.gene017582 | MS.gene018718 | PPI |
| MS.gene049886 | MS.gene017582 | PPI |
| MS.gene23495 | MS.gene017582 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene017582.t1 | MTR_8g074640 | 91.534 | 378 | 31 | 1 | 1 | 378 | 1 | 377 | 0.0 | 706 |
| MS.gene017582.t1 | MTR_8g074610 | 77.105 | 380 | 83 | 4 | 1 | 378 | 20 | 397 | 0.0 | 595 |
| MS.gene017582.t1 | MTR_2g041510 | 76.115 | 381 | 79 | 5 | 1 | 378 | 21 | 392 | 0.0 | 559 |
| MS.gene017582.t1 | MTR_2g041510 | 76.115 | 381 | 79 | 5 | 1 | 378 | 1 | 372 | 0.0 | 559 |
| MS.gene017582.t1 | MTR_5g079830 | 35.593 | 354 | 179 | 12 | 1 | 340 | 1 | 319 | 1.25e-51 | 177 |
| MS.gene017582.t1 | MTR_6g092570 | 34.211 | 380 | 193 | 11 | 2 | 364 | 4 | 343 | 8.24e-50 | 171 |
| MS.gene017582.t1 | MTR_8g085760 | 33.684 | 380 | 201 | 12 | 2 | 354 | 9 | 364 | 8.46e-49 | 169 |
| MS.gene017582.t1 | MTR_7g096720 | 32.682 | 358 | 192 | 11 | 8 | 340 | 15 | 348 | 2.89e-45 | 160 |
| MS.gene017582.t1 | MTR_4g088925 | 32.258 | 372 | 198 | 12 | 14 | 364 | 34 | 372 | 1.74e-39 | 144 |
| MS.gene017582.t1 | MTR_1g026850 | 30.227 | 397 | 222 | 13 | 1 | 376 | 1 | 363 | 2.55e-36 | 135 |
| MS.gene017582.t1 | MTR_3g030050 | 30.166 | 421 | 230 | 14 | 5 | 376 | 15 | 420 | 4.91e-35 | 133 |
| MS.gene017582.t1 | MTR_6g018390 | 29.722 | 360 | 189 | 13 | 5 | 340 | 4 | 323 | 5.94e-30 | 118 |
| MS.gene017582.t1 | MTR_3g058460 | 31.443 | 388 | 222 | 15 | 6 | 376 | 15 | 375 | 3.29e-29 | 116 |
| MS.gene017582.t1 | MTR_3g030360 | 29.620 | 368 | 204 | 14 | 3 | 351 | 2 | 333 | 4.57e-28 | 113 |
| MS.gene017582.t1 | MTR_3g029650 | 25.956 | 366 | 186 | 8 | 5 | 340 | 4 | 314 | 1.86e-27 | 114 |
| MS.gene017582.t1 | MTR_3g029650 | 25.556 | 360 | 228 | 13 | 4 | 342 | 370 | 710 | 8.94e-20 | 91.7 |
| MS.gene017582.t1 | MTR_1g007260 | 27.907 | 387 | 210 | 15 | 5 | 359 | 21 | 370 | 2.79e-26 | 108 |
| MS.gene017582.t1 | MTR_2g047180 | 28.205 | 390 | 235 | 14 | 5 | 376 | 3 | 365 | 4.50e-26 | 108 |
| MS.gene017582.t1 | MTR_4g125090 | 28.354 | 328 | 172 | 11 | 2 | 321 | 41 | 313 | 1.35e-25 | 105 |
| MS.gene017582.t1 | MTR_3g029620 | 31.680 | 363 | 193 | 16 | 5 | 345 | 2 | 331 | 1.96e-25 | 106 |
| MS.gene017582.t1 | MTR_6g027040 | 27.930 | 401 | 235 | 18 | 5 | 378 | 4 | 377 | 1.24e-24 | 103 |
| MS.gene017582.t1 | MTR_1g016570 | 29.070 | 344 | 187 | 12 | 6 | 342 | 21 | 314 | 7.50e-24 | 101 |
| MS.gene017582.t1 | MTR_3g029920 | 29.275 | 386 | 204 | 16 | 4 | 361 | 7 | 351 | 8.86e-24 | 101 |
| MS.gene017582.t1 | MTR_6g027100 | 28.678 | 401 | 232 | 19 | 5 | 378 | 4 | 377 | 9.29e-24 | 102 |
| MS.gene017582.t1 | MTR_3g030440 | 28.530 | 347 | 209 | 12 | 1 | 342 | 1 | 313 | 2.19e-23 | 100 |
| MS.gene017582.t1 | MTR_6g018400 | 26.768 | 396 | 201 | 15 | 3 | 362 | 2 | 344 | 5.13e-23 | 99.4 |
| MS.gene017582.t1 | MTR_3g030480 | 29.226 | 349 | 196 | 11 | 5 | 342 | 4 | 312 | 6.11e-23 | 99.0 |
| MS.gene017582.t1 | MTR_3g030380 | 26.801 | 347 | 211 | 9 | 4 | 342 | 3 | 314 | 3.14e-22 | 97.1 |
| MS.gene017582.t1 | MTR_3g029640 | 28.533 | 368 | 188 | 15 | 5 | 345 | 3 | 322 | 7.29e-22 | 96.3 |
| MS.gene017582.t1 | MTR_6g018360 | 29.086 | 361 | 204 | 17 | 5 | 342 | 4 | 335 | 1.12e-21 | 96.3 |
| MS.gene017582.t1 | MTR_1g049170 | 28.117 | 377 | 199 | 15 | 1 | 342 | 1 | 340 | 2.09e-21 | 95.1 |
| MS.gene017582.t1 | MTR_3g037710 | 36.905 | 168 | 98 | 3 | 180 | 342 | 55 | 219 | 9.68e-21 | 91.3 |
| MS.gene017582.t1 | MTR_3g114990 | 27.000 | 400 | 232 | 16 | 5 | 374 | 22 | 391 | 1.21e-20 | 93.2 |
| MS.gene017582.t1 | MTR_3g029840 | 28.134 | 359 | 194 | 16 | 5 | 342 | 4 | 319 | 1.38e-20 | 92.4 |
| MS.gene017582.t1 | MTR_3g030080 | 27.095 | 358 | 202 | 15 | 6 | 342 | 7 | 326 | 3.28e-20 | 91.3 |
| MS.gene017582.t1 | MTR_3g029950 | 27.824 | 363 | 197 | 16 | 5 | 345 | 4 | 323 | 1.43e-19 | 89.4 |
| MS.gene017582.t1 | MTR_3g114970 | 25.337 | 371 | 231 | 16 | 5 | 363 | 3 | 339 | 2.20e-19 | 89.0 |
| MS.gene017582.t1 | MTR_3g029670 | 24.390 | 369 | 221 | 9 | 5 | 343 | 4 | 344 | 5.91e-19 | 87.8 |
| MS.gene017582.t1 | MTR_3g030355 | 26.812 | 414 | 211 | 18 | 1 | 359 | 1 | 377 | 2.86e-18 | 85.9 |
| MS.gene017582.t1 | MTR_3g091990 | 26.648 | 364 | 212 | 13 | 4 | 340 | 5 | 340 | 2.99e-18 | 86.3 |
| MS.gene017582.t1 | MTR_3g029750 | 23.545 | 378 | 229 | 8 | 5 | 351 | 4 | 352 | 4.20e-18 | 85.5 |
| MS.gene017582.t1 | MTR_3g029820 | 28.934 | 394 | 160 | 17 | 4 | 370 | 7 | 307 | 2.30e-16 | 79.7 |
| MS.gene017582.t1 | MTR_3g047980 | 27.926 | 376 | 220 | 16 | 6 | 376 | 30 | 359 | 7.96e-16 | 78.6 |
| MS.gene017582.t1 | MTR_6g018315 | 26.667 | 390 | 231 | 19 | 18 | 377 | 2 | 366 | 2.66e-15 | 77.0 |
| MS.gene017582.t1 | MTR_3g029900 | 28.846 | 312 | 159 | 14 | 68 | 368 | 177 | 436 | 5.13e-15 | 76.6 |
| MS.gene017582.t1 | MTR_1g016540 | 34.965 | 143 | 75 | 4 | 207 | 342 | 2 | 133 | 4.38e-12 | 64.7 |
| MS.gene017582.t1 | MTR_6g018370 | 33.594 | 128 | 76 | 4 | 192 | 318 | 132 | 251 | 5.84e-11 | 62.8 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene017582.t1 | AT2G17030 | 31.135 | 379 | 224 | 13 | 5 | 361 | 2 | 365 | 2.85e-40 | 147 |
| MS.gene017582.t1 | AT1G65770 | 30.252 | 357 | 219 | 11 | 5 | 354 | 2 | 335 | 6.37e-36 | 134 |
| MS.gene017582.t1 | AT4G35733 | 29.600 | 375 | 208 | 12 | 1 | 356 | 1 | 338 | 6.66e-35 | 132 |
| MS.gene017582.t1 | AT2G17036 | 27.614 | 373 | 239 | 11 | 5 | 361 | 2 | 359 | 1.70e-29 | 118 |
| MS.gene017582.t1 | AT2G17036 | 27.614 | 373 | 239 | 11 | 5 | 361 | 1 | 358 | 1.80e-29 | 118 |
| MS.gene017582.t1 | AT3G25750 | 28.760 | 379 | 205 | 14 | 3 | 356 | 2 | 340 | 3.63e-29 | 116 |
| MS.gene017582.t1 | AT1G44080 | 27.688 | 372 | 202 | 10 | 4 | 358 | 3 | 324 | 5.92e-29 | 115 |
| MS.gene017582.t1 | AT1G65740 | 29.041 | 365 | 225 | 12 | 5 | 354 | 2 | 347 | 4.08e-27 | 110 |
| MS.gene017582.t1 | AT3G25750 | 28.117 | 377 | 196 | 13 | 3 | 356 | 2 | 326 | 5.55e-26 | 107 |
| MS.gene017582.t1 | AT2G26160 | 28.531 | 354 | 204 | 12 | 3 | 342 | 2 | 320 | 5.75e-25 | 104 |
| MS.gene017582.t1 | AT1G65760 | 28.686 | 373 | 216 | 15 | 6 | 359 | 5 | 346 | 1.40e-23 | 100 |
| MS.gene017582.t1 | AT2G17690 | 27.423 | 423 | 218 | 14 | 6 | 359 | 3 | 405 | 1.47e-21 | 95.9 |
| MS.gene017582.t1 | AT2G16290 | 24.103 | 390 | 236 | 14 | 5 | 356 | 2 | 369 | 1.50e-18 | 87.0 |
| MS.gene017582.t1 | AT5G60060 | 28.307 | 378 | 219 | 19 | 7 | 361 | 11 | 359 | 3.28e-18 | 85.5 |
| MS.gene017582.t1 | AT1G64840 | 26.891 | 357 | 208 | 16 | 6 | 342 | 4 | 327 | 1.73e-16 | 80.5 |
| MS.gene017582.t1 | AT5G24040 | 23.499 | 383 | 198 | 17 | 5 | 346 | 2 | 330 | 1.11e-13 | 72.0 |
| MS.gene017582.t1 | AT1G57906 | 29.545 | 176 | 84 | 6 | 187 | 329 | 58 | 226 | 3.40e-12 | 65.9 |
Find 87 sgRNAs with CRISPR-Local
Find 93 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TGTAGCTTGTTGTATCTTTC+AGG | 0.173014 | 8.3:+28582291 | MS.gene017582:CDS |
| TTCAACAAGTTCCATATAAT+TGG | 0.175089 | 8.3:-28582151 | None:intergenic |
| AGGGAATTGCATCTACTTTA+AGG | 0.185868 | 8.3:+28582756 | MS.gene017582:CDS |
| TATGGAACTTGTTGAATTAT+AGG | 0.195427 | 8.3:+28582158 | MS.gene017582:CDS |
| AAAGGAAAAGAAGGAGAATT+TGG | 0.229744 | 8.3:-28581893 | None:intergenic |
| GTTTCTGCTAAAGAATTAAT+TGG | 0.230909 | 8.3:+28582727 | MS.gene017582:CDS |
| ATCTTGGACCATAGCTCTTT+GGG | 0.243195 | 8.3:-28581785 | None:intergenic |
| ATCTTTCAGGAAGGTAAATT+AGG | 0.270135 | 8.3:+28582304 | MS.gene017582:CDS |
| TATCTTGGACCATAGCTCTT+TGG | 0.291416 | 8.3:-28581786 | None:intergenic |
| ATGGAACTTGTTGAATTATA+GGG | 0.301160 | 8.3:+28582159 | MS.gene017582:CDS |
| TCACCTCCTTTGTGTGGGTT+TGG | 0.311829 | 8.3:+28582481 | MS.gene017582:CDS |
| TGAAATATGGAGACACAAAA+TGG | 0.318037 | 8.3:+28582332 | MS.gene017582:CDS |
| AAATTAGGATTCATGAAATA+TGG | 0.332072 | 8.3:+28582319 | MS.gene017582:CDS |
| ATCAAGATACTTCCCTATCT+TGG | 0.333662 | 8.3:-28581801 | None:intergenic |
| ACAACAGTTCTCCAATTATA+TGG | 0.333718 | 8.3:+28582140 | MS.gene017582:CDS |
| TTGATAGAGGTAATTGTATA+GGG | 0.347302 | 8.3:-28581942 | None:intergenic |
| CTCACAAAACTTGATGAGTA+TGG | 0.352709 | 8.3:-28582220 | None:intergenic |
| ACTCTCCTTGGATTAATGTT+GGG | 0.357299 | 8.3:+28582263 | MS.gene017582:CDS |
| ATTGATAGAGGTAATTGTAT+AGG | 0.365166 | 8.3:-28581943 | None:intergenic |
| CACTTTGATCAACCAACCTT+TGG | 0.369704 | 8.3:-28582038 | None:intergenic |
| AAAGAGCTATGGTCCAAGAT+AGG | 0.372622 | 8.3:+28581788 | MS.gene017582:CDS |
| ATAAATGGGGAACTATTTCA+TGG | 0.373052 | 8.3:+28582425 | MS.gene017582:CDS |
| CTTGCATTGACAAGACTTTG+TGG | 0.374534 | 8.3:+28582842 | MS.gene017582:CDS |
| AGGTAATTGTATAGGGAGAC+AGG | 0.376170 | 8.3:-28581935 | None:intergenic |
| ACATTAATCCAAGGAGAGTT+AGG | 0.379494 | 8.3:-28582259 | None:intergenic |
| AAAGAATTAATTGGATATCA+AGG | 0.389911 | 8.3:+28582736 | MS.gene017582:CDS |
| GATAATTTGCAGTCGGAGAT+AGG | 0.391629 | 8.3:-28582100 | None:intergenic |
| TTTATATGTTGGATGAAGAA+TGG | 0.404503 | 8.3:+28582635 | MS.gene017582:CDS |
| CCATGATCGGGACGCGGTTG+TGG | 0.420452 | 8.3:+28582600 | MS.gene017582:CDS |
| ATTTGGAAGAGAGGGAGGAA+TGG | 0.422238 | 8.3:-28581876 | None:intergenic |
| TGTCCTATCCTAACTCTCCT+TGG | 0.428803 | 8.3:+28582251 | MS.gene017582:CDS |
| TGATGATGTCATTGTATTCA+AGG | 0.430249 | 8.3:+28582384 | MS.gene017582:CDS |
| TTATATGTTGGATGAAGAAT+GGG | 0.432756 | 8.3:+28582636 | MS.gene017582:CDS |
| GATAAATAGTTGATTGATAG+AGG | 0.439760 | 8.3:-28581955 | None:intergenic |
| CTCTCCTTGGATTAATGTTG+GGG | 0.439895 | 8.3:+28582264 | MS.gene017582:CDS |
| AACATTTGGTGGAATCTTGT+GGG | 0.442266 | 8.3:+28582512 | MS.gene017582:CDS |
| GTTTGGAAACAAGAAACATT+TGG | 0.451619 | 8.3:+28582498 | MS.gene017582:CDS |
| AACTCTCCTTGGATTAATGT+TGG | 0.455668 | 8.3:+28582262 | MS.gene017582:CDS |
| GAAGGAGAATTTGGAAGAGA+GGG | 0.458355 | 8.3:-28581884 | None:intergenic |
| AAGTTGGAACTTGCATTAGT+TGG | 0.460926 | 8.3:-28581986 | None:intergenic |
| AAACATTTGGTGGAATCTTG+TGG | 0.483180 | 8.3:+28582511 | MS.gene017582:CDS |
| CACGTTGGATGAGATGTTAG+AGG | 0.485509 | 8.3:+28582872 | MS.gene017582:CDS |
| TGTTGGAAGACGCCATGATC+GGG | 0.485748 | 8.3:+28582588 | MS.gene017582:CDS |
| AGAAGGAGAATTTGGAAGAG+AGG | 0.489920 | 8.3:-28581885 | None:intergenic |
| CCACAACCGCGTCCCGATCA+TGG | 0.492597 | 8.3:-28582600 | None:intergenic |
| GGTATTTGTAAAGGAAAAGA+AGG | 0.498247 | 8.3:-28581902 | None:intergenic |
| ACAACTGCTGCTTAAAATGA+AGG | 0.500009 | 8.3:-28582695 | None:intergenic |
| CACCTTCTTATACCAAAGGT+TGG | 0.500177 | 8.3:+28582026 | MS.gene017582:CDS |
| TAATGCTCGTATGTACAGCT+TGG | 0.504456 | 8.3:+28582786 | MS.gene017582:CDS |
| GGAGAATTTGGAAGAGAGGG+AGG | 0.512512 | 8.3:-28581881 | None:intergenic |
| AACCAACCTTTGGTATAAGA+AGG | 0.522904 | 8.3:-28582028 | None:intergenic |
| AACATGAGAGGGAACAATGT+TGG | 0.526890 | 8.3:+28582571 | MS.gene017582:CDS |
| TAAAGGTGGTAAATTGAAGT+TGG | 0.535440 | 8.3:-28582002 | None:intergenic |
| TTGGTATAAGAAGGTGCTAA+AGG | 0.538170 | 8.3:-28582019 | None:intergenic |
| AAGAGCTATGGTCCAAGATA+GGG | 0.546977 | 8.3:+28581789 | MS.gene017582:CDS |
| CTCATCTCCCCAAAGAGCTA+TGG | 0.548235 | 8.3:+28581777 | MS.gene017582:CDS |
| GGACTCAACAAACTCTTCAA+AGG | 0.551008 | 8.3:-28582079 | None:intergenic |
| GTGTTTCAAAGTTTATATGT+TGG | 0.555002 | 8.3:+28582624 | MS.gene017582:CDS |
| ATGTTGGAAGACGCCATGAT+CGG | 0.555148 | 8.3:+28582587 | MS.gene017582:CDS |
| GGTAATTGTATAGGGAGACA+GGG | 0.555775 | 8.3:-28581934 | None:intergenic |
| AAGAATTAATTGGATATCAA+GGG | 0.561141 | 8.3:+28582737 | MS.gene017582:CDS |
| ATGGTGCGAGGTATTTGTAA+AGG | 0.569151 | 8.3:-28581911 | None:intergenic |
| TGGAAACAAGAAACATTTGG+TGG | 0.570071 | 8.3:+28582501 | MS.gene017582:CDS |
| TTAGCACCTTCTTATACCAA+AGG | 0.570602 | 8.3:+28582022 | MS.gene017582:CDS |
| CAAAGGTTGGTTGATCAAAG+TGG | 0.574189 | 8.3:+28582039 | MS.gene017582:CDS |
| CACAAAGTCTTGTCAATGCA+AGG | 0.575490 | 8.3:-28582841 | None:intergenic |
| GTTGTTGATAGATACTATGA+AGG | 0.579405 | 8.3:+28582544 | MS.gene017582:CDS |
| ATGAAGGTGAAAACATGAGA+GGG | 0.582267 | 8.3:+28582560 | MS.gene017582:CDS |
| GATGATGTCATTGTATTCAA+GGG | 0.582955 | 8.3:+28582385 | MS.gene017582:CDS |
| AGGATATGATAATTTGCAGT+CGG | 0.584721 | 8.3:-28582107 | None:intergenic |
| CTTTGTGGTCTCATTCACGT+TGG | 0.590681 | 8.3:+28582857 | MS.gene017582:CDS |
| GCTTGTTGTATCTTTCAGGA+AGG | 0.596136 | 8.3:+28582295 | MS.gene017582:CDS |
| GTATAAGAAGGTGCTAAAGG+TGG | 0.604135 | 8.3:-28582016 | None:intergenic |
| AAGACGCCATGATCGGGACG+CGG | 0.613949 | 8.3:+28582594 | MS.gene017582:CDS |
| CAATCCCCAACATTAATCCA+AGG | 0.626001 | 8.3:-28582268 | None:intergenic |
| TCTTGGACCATAGCTCTTTG+GGG | 0.628895 | 8.3:-28581784 | None:intergenic |
| GAACTGTTGTTACTGTCAAG+AGG | 0.629902 | 8.3:-28582127 | None:intergenic |
| TATATGTTGGATGAAGAATG+GGG | 0.631666 | 8.3:+28582637 | MS.gene017582:CDS |
| ATTGTATAGGGAGACAGGGA+TGG | 0.639587 | 8.3:-28581930 | None:intergenic |
| AATCCAAGGAGAGTTAGGAT+AGG | 0.641071 | 8.3:-28582254 | None:intergenic |
| AGGGAGACAGGGATGGTGCG+AGG | 0.646104 | 8.3:-28581923 | None:intergenic |
| ATATGTTGGATGAAGAATGG+GGG | 0.646805 | 8.3:+28582638 | MS.gene017582:CDS |
| TATGAAGGTGAAAACATGAG+AGG | 0.647138 | 8.3:+28582559 | MS.gene017582:CDS |
| TTTCCAAACCCACACAAAGG+AGG | 0.652810 | 8.3:-28582484 | None:intergenic |
| AATGCAAGGATCAAAATCAA+CGG | 0.653913 | 8.3:-28582827 | None:intergenic |
| AGACACAAAATGGAAACTTG+TGG | 0.657681 | 8.3:+28582342 | MS.gene017582:CDS |
| TTGTTTCCAAACCCACACAA+AGG | 0.659541 | 8.3:-28582487 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AAAGAATTAATTGGATATCA+AGG | + | chr8.3:28582736-28582755 | MS.gene017582:CDS | 20.0% |
| !! | AAATTAGGATTCATGAAATA+TGG | + | chr8.3:28582319-28582338 | MS.gene017582:CDS | 20.0% |
| !! | AAGAATTAATTGGATATCAA+GGG | + | chr8.3:28582737-28582756 | MS.gene017582:CDS | 20.0% |
| !!! | AGTTTTATGTTACTGATAAA+TGG | + | chr8.3:28582410-28582429 | MS.gene017582:CDS | 20.0% |
| !!! | GTTTTATGTTACTGATAAAT+GGG | + | chr8.3:28582411-28582430 | MS.gene017582:CDS | 20.0% |
| !!! | TTTTATGTTACTGATAAATG+GGG | + | chr8.3:28582412-28582431 | MS.gene017582:CDS | 20.0% |
| ! | ATTGATAGAGGTAATTGTAT+AGG | - | chr8.3:28581946-28581965 | None:intergenic | 25.0% |
| ! | GATAAATAGTTGATTGATAG+AGG | - | chr8.3:28581958-28581977 | None:intergenic | 25.0% |
| ! | GTGTTTCAAAGTTTATATGT+TGG | + | chr8.3:28582624-28582643 | MS.gene017582:CDS | 25.0% |
| ! | GTTTCTGCTAAAGAATTAAT+TGG | + | chr8.3:28582727-28582746 | MS.gene017582:CDS | 25.0% |
| ! | TTATATGTTGGATGAAGAAT+GGG | + | chr8.3:28582636-28582655 | MS.gene017582:CDS | 25.0% |
| ! | TTCAACAAGTTCCATATAAT+TGG | - | chr8.3:28582154-28582173 | None:intergenic | 25.0% |
| ! | TTGATAGAGGTAATTGTATA+GGG | - | chr8.3:28581945-28581964 | None:intergenic | 25.0% |
| ! | TTTATATGTTGGATGAAGAA+TGG | + | chr8.3:28582635-28582654 | MS.gene017582:CDS | 25.0% |
| !!! | ATGGAACTTGTTGAATTATA+GGG | + | chr8.3:28582159-28582178 | MS.gene017582:CDS | 25.0% |
| !!! | TATGGAACTTGTTGAATTAT+AGG | + | chr8.3:28582158-28582177 | MS.gene017582:CDS | 25.0% |
| AAAGGAAAAGAAGGAGAATT+TGG | - | chr8.3:28581896-28581915 | None:intergenic | 30.0% | |
| AATGCAAGGATCAAAATCAA+CGG | - | chr8.3:28582830-28582849 | None:intergenic | 30.0% | |
| ACAACAGTTCTCCAATTATA+TGG | + | chr8.3:28582140-28582159 | MS.gene017582:CDS | 30.0% | |
| ATAAATGGGGAACTATTTCA+TGG | + | chr8.3:28582425-28582444 | MS.gene017582:CDS | 30.0% | |
| ATCTTTCAGGAAGGTAAATT+AGG | + | chr8.3:28582304-28582323 | MS.gene017582:CDS | 30.0% | |
| GATGATGTCATTGTATTCAA+GGG | + | chr8.3:28582385-28582404 | MS.gene017582:CDS | 30.0% | |
| GGTATTTGTAAAGGAAAAGA+AGG | - | chr8.3:28581905-28581924 | None:intergenic | 30.0% | |
| GTTTGGAAACAAGAAACATT+TGG | + | chr8.3:28582498-28582517 | MS.gene017582:CDS | 30.0% | |
| TAAAGGTGGTAAATTGAAGT+TGG | - | chr8.3:28582005-28582024 | None:intergenic | 30.0% | |
| TATATGTTGGATGAAGAATG+GGG | + | chr8.3:28582637-28582656 | MS.gene017582:CDS | 30.0% | |
| TGAAATATGGAGACACAAAA+TGG | + | chr8.3:28582332-28582351 | MS.gene017582:CDS | 30.0% | |
| ! | AGGATATGATAATTTGCAGT+CGG | - | chr8.3:28582110-28582129 | None:intergenic | 30.0% |
| ! | GTTGTTGATAGATACTATGA+AGG | + | chr8.3:28582544-28582563 | MS.gene017582:CDS | 30.0% |
| ! | TGATGATGTCATTGTATTCA+AGG | + | chr8.3:28582384-28582403 | MS.gene017582:CDS | 30.0% |
| AACCAACCTTTGGTATAAGA+AGG | - | chr8.3:28582031-28582050 | None:intergenic | 35.0% | |
| AACTCTCCTTGGATTAATGT+TGG | + | chr8.3:28582262-28582281 | MS.gene017582:CDS | 35.0% | |
| AAGTTGGAACTTGCATTAGT+TGG | - | chr8.3:28581989-28582008 | None:intergenic | 35.0% | |
| ACATTAATCCAAGGAGAGTT+AGG | - | chr8.3:28582262-28582281 | None:intergenic | 35.0% | |
| ACTCTCCTTGGATTAATGTT+GGG | + | chr8.3:28582263-28582282 | MS.gene017582:CDS | 35.0% | |
| AGACACAAAATGGAAACTTG+TGG | + | chr8.3:28582342-28582361 | MS.gene017582:CDS | 35.0% | |
| AGGGAATTGCATCTACTTTA+AGG | + | chr8.3:28582756-28582775 | MS.gene017582:CDS | 35.0% | |
| ATATGTTGGATGAAGAATGG+GGG | + | chr8.3:28582638-28582657 | MS.gene017582:CDS | 35.0% | |
| ATCAAGATACTTCCCTATCT+TGG | - | chr8.3:28581804-28581823 | None:intergenic | 35.0% | |
| ATGAAGGTGAAAACATGAGA+GGG | + | chr8.3:28582560-28582579 | MS.gene017582:CDS | 35.0% | |
| CTCACAAAACTTGATGAGTA+TGG | - | chr8.3:28582223-28582242 | None:intergenic | 35.0% | |
| TATGAAGGTGAAAACATGAG+AGG | + | chr8.3:28582559-28582578 | MS.gene017582:CDS | 35.0% | |
| TGGAAACAAGAAACATTTGG+TGG | + | chr8.3:28582501-28582520 | MS.gene017582:CDS | 35.0% | |
| TTAGCACCTTCTTATACCAA+AGG | + | chr8.3:28582022-28582041 | MS.gene017582:CDS | 35.0% | |
| ! | AAACATTTGGTGGAATCTTG+TGG | + | chr8.3:28582511-28582530 | MS.gene017582:CDS | 35.0% |
| ! | AACATTTGGTGGAATCTTGT+GGG | + | chr8.3:28582512-28582531 | MS.gene017582:CDS | 35.0% |
| ! | AATTTTCACCTCCTTTGTGT+GGG | + | chr8.3:28582476-28582495 | MS.gene017582:CDS | 35.0% |
| ! | ACAACTGCTGCTTAAAATGA+AGG | - | chr8.3:28582698-28582717 | None:intergenic | 35.0% |
| !! | TGTAGCTTGTTGTATCTTTC+AGG | + | chr8.3:28582291-28582310 | MS.gene017582:CDS | 35.0% |
| !! | TTGGTATAAGAAGGTGCTAA+AGG | - | chr8.3:28582022-28582041 | None:intergenic | 35.0% |
| AAAGAGCTATGGTCCAAGAT+AGG | + | chr8.3:28581788-28581807 | MS.gene017582:CDS | 40.0% | |
| AACATGAGAGGGAACAATGT+TGG | + | chr8.3:28582571-28582590 | MS.gene017582:CDS | 40.0% | |
| AAGAGCTATGGTCCAAGATA+GGG | + | chr8.3:28581789-28581808 | MS.gene017582:CDS | 40.0% | |
| AATCCAAGGAGAGTTAGGAT+AGG | - | chr8.3:28582257-28582276 | None:intergenic | 40.0% | |
| AGAAGGAGAATTTGGAAGAG+AGG | - | chr8.3:28581888-28581907 | None:intergenic | 40.0% | |
| AGGTAATTGTATAGGGAGAC+AGG | - | chr8.3:28581938-28581957 | None:intergenic | 40.0% | |
| ATCTTGGACCATAGCTCTTT+GGG | - | chr8.3:28581788-28581807 | None:intergenic | 40.0% | |
| CAATCCCCAACATTAATCCA+AGG | - | chr8.3:28582271-28582290 | None:intergenic | 40.0% | |
| CACCTTCTTATACCAAAGGT+TGG | + | chr8.3:28582026-28582045 | MS.gene017582:CDS | 40.0% | |
| CACTTTGATCAACCAACCTT+TGG | - | chr8.3:28582041-28582060 | None:intergenic | 40.0% | |
| CTCTCCTTGGATTAATGTTG+GGG | + | chr8.3:28582264-28582283 | MS.gene017582:CDS | 40.0% | |
| GAACTGTTGTTACTGTCAAG+AGG | - | chr8.3:28582130-28582149 | None:intergenic | 40.0% | |
| GAAGGAGAATTTGGAAGAGA+GGG | - | chr8.3:28581887-28581906 | None:intergenic | 40.0% | |
| GGACTCAACAAACTCTTCAA+AGG | - | chr8.3:28582082-28582101 | None:intergenic | 40.0% | |
| GGTAATTGTATAGGGAGACA+GGG | - | chr8.3:28581937-28581956 | None:intergenic | 40.0% | |
| TAATGCTCGTATGTACAGCT+TGG | + | chr8.3:28582786-28582805 | MS.gene017582:CDS | 40.0% | |
| TATCTTGGACCATAGCTCTT+TGG | - | chr8.3:28581789-28581808 | None:intergenic | 40.0% | |
| TTGTTTCCAAACCCACACAA+AGG | - | chr8.3:28582490-28582509 | None:intergenic | 40.0% | |
| ! | ATGGTGCGAGGTATTTGTAA+AGG | - | chr8.3:28581914-28581933 | None:intergenic | 40.0% |
| ! | CAAAGGTTGGTTGATCAAAG+TGG | + | chr8.3:28582039-28582058 | MS.gene017582:CDS | 40.0% |
| ! | CAATTTTCACCTCCTTTGTG+TGG | + | chr8.3:28582475-28582494 | MS.gene017582:CDS | 40.0% |
| ! | CACAAAGTCTTGTCAATGCA+AGG | - | chr8.3:28582844-28582863 | None:intergenic | 40.0% |
| ! | CTTGCATTGACAAGACTTTG+TGG | + | chr8.3:28582842-28582861 | MS.gene017582:CDS | 40.0% |
| ! | GATAATTTGCAGTCGGAGAT+AGG | - | chr8.3:28582103-28582122 | None:intergenic | 40.0% |
| !! | GCTTGTTGTATCTTTCAGGA+AGG | + | chr8.3:28582295-28582314 | MS.gene017582:CDS | 40.0% |
| !! | GTATAAGAAGGTGCTAAAGG+TGG | - | chr8.3:28582019-28582038 | None:intergenic | 40.0% |
| ATGTTGGAAGACGCCATGAT+CGG | + | chr8.3:28582587-28582606 | MS.gene017582:CDS | 45.0% | |
| ATTGTATAGGGAGACAGGGA+TGG | - | chr8.3:28581933-28581952 | None:intergenic | 45.0% | |
| ATTTGGAAGAGAGGGAGGAA+TGG | - | chr8.3:28581879-28581898 | None:intergenic | 45.0% | |
| CTTTGTGGTCTCATTCACGT+TGG | + | chr8.3:28582857-28582876 | MS.gene017582:CDS | 45.0% | |
| GGATGAAGAATGGGGGAAAT+GGG | + | chr8.3:28582645-28582664 | MS.gene017582:CDS | 45.0% | |
| TCTTGGACCATAGCTCTTTG+GGG | - | chr8.3:28581787-28581806 | None:intergenic | 45.0% | |
| TGGATGAAGAATGGGGGAAA+TGG | + | chr8.3:28582644-28582663 | MS.gene017582:CDS | 45.0% | |
| TGTCCTATCCTAACTCTCCT+TGG | + | chr8.3:28582251-28582270 | MS.gene017582:CDS | 45.0% | |
| TTTCCAAACCCACACAAAGG+AGG | - | chr8.3:28582487-28582506 | None:intergenic | 45.0% | |
| CTCATCTCCCCAAAGAGCTA+TGG | + | chr8.3:28581777-28581796 | MS.gene017582:CDS | 50.0% | |
| GGAGAATTTGGAAGAGAGGG+AGG | - | chr8.3:28581884-28581903 | None:intergenic | 50.0% | |
| TCACCTCCTTTGTGTGGGTT+TGG | + | chr8.3:28582481-28582500 | MS.gene017582:CDS | 50.0% | |
| TGTTGGAAGACGCCATGATC+GGG | + | chr8.3:28582588-28582607 | MS.gene017582:CDS | 50.0% | |
| AAGACGCCATGATCGGGACG+CGG | + | chr8.3:28582594-28582613 | MS.gene017582:CDS | 60.0% | |
| AGGGAGACAGGGATGGTGCG+AGG | - | chr8.3:28581926-28581945 | None:intergenic | 65.0% | |
| CCACAACCGCGTCCCGATCA+TGG | - | chr8.3:28582603-28582622 | None:intergenic | 65.0% | |
| CCATGATCGGGACGCGGTTG+TGG | + | chr8.3:28582600-28582619 | MS.gene017582:CDS | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr8.3 | gene | 28581755 | 28582891 | 28581755 | ID=MS.gene017582 |
| chr8.3 | mRNA | 28581755 | 28582891 | 28581755 | ID=MS.gene017582.t1;Parent=MS.gene017582 |
| chr8.3 | exon | 28581755 | 28582891 | 28581755 | ID=MS.gene017582.t1.exon1;Parent=MS.gene017582.t1 |
| chr8.3 | CDS | 28581755 | 28582891 | 28581755 | ID=cds.MS.gene017582.t1;Parent=MS.gene017582.t1 |
| Gene Sequence |
| Protein sequence |
