AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene23495


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene23495.t1 MTR_7g096720 67.810 379 110 5 1 369 6 382 3.94e-175 492
MS.gene23495.t1 MTR_4g088925 63.128 358 118 6 16 369 34 381 5.78e-143 410
MS.gene23495.t1 MTR_8g085760 54.688 384 150 8 1 367 6 382 9.68e-131 380
MS.gene23495.t1 MTR_6g092570 46.027 365 158 14 12 365 12 348 2.48e-81 253
MS.gene23495.t1 MTR_5g079830 43.207 368 165 14 12 362 10 350 1.32e-72 231
MS.gene23495.t1 MTR_8g074640 36.559 372 192 12 1 348 1 352 1.94e-55 186
MS.gene23495.t1 MTR_8g074610 37.731 379 186 15 10 360 27 383 1.97e-55 187
MS.gene23495.t1 MTR_2g041510 36.292 383 196 13 1 360 1 358 8.03e-53 179
MS.gene23495.t1 MTR_2g041510 36.719 384 193 14 1 360 21 378 1.47e-52 179
MS.gene23495.t1 MTR_4g125090 39.809 314 135 13 12 318 49 315 5.60e-49 167
MS.gene23495.t1 MTR_7g096760 81.915 94 16 1 216 308 1 94 1.24e-44 150
MS.gene23495.t1 MTR_3g030050 25.472 424 220 16 10 361 18 417 2.61e-21 95.1
MS.gene23495.t1 MTR_3g058460 25.714 385 224 14 10 361 17 372 5.42e-21 93.6
MS.gene23495.t1 MTR_3g029650 28.736 348 194 14 6 335 3 314 3.87e-18 86.7
MS.gene23495.t1 MTR_2g047180 23.724 392 224 14 10 361 6 362 7.06e-18 84.3
MS.gene23495.t1 MTR_1g026850 25.995 377 230 15 10 361 8 360 1.42e-17 83.6
MS.gene23495.t1 MTR_6g018400 25.419 358 189 12 10 333 7 320 1.54e-17 83.6
MS.gene23495.t1 MTR_3g030080 24.937 397 216 17 10 361 9 368 6.78e-16 78.6
MS.gene23495.t1 MTR_3g029920 24.857 350 204 12 12 335 13 329 7.56e-13 69.7
MS.gene23495.t1 MTR_3g114990 24.507 355 225 13 10 339 25 361 1.04e-12 69.3
MS.gene23495.t1 MTR_3g029800 23.988 346 207 11 10 335 6 315 1.44e-11 65.5
MS.gene23495.t1 MTR_3g029640 23.096 394 224 14 10 362 6 361 7.94e-11 63.2
MS.gene23495.t1 MTR_3g030560 23.796 353 210 12 10 335 6 326 9.29e-11 63.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene23495.t1 AT1G44080 29.360 344 207 8 10 348 7 319 1.79e-33 127
MS.gene23495.t1 AT1G65770 28.453 362 204 12 10 348 5 334 7.45e-33 126
MS.gene23495.t1 AT2G26160 28.571 343 221 8 10 347 7 330 2.52e-32 124
MS.gene23495.t1 AT1G65740 27.577 359 223 9 10 348 5 346 1.53e-31 122
MS.gene23495.t1 AT3G25750 30.088 339 184 13 10 335 7 305 1.23e-29 117
MS.gene23495.t1 AT3G25750 28.824 340 201 11 10 335 7 319 2.69e-28 114
MS.gene23495.t1 AT4G35733 28.000 350 215 10 10 346 8 333 1.88e-27 111
MS.gene23495.t1 AT1G65760 27.701 361 190 11 6 335 3 323 2.15e-27 111
MS.gene23495.t1 AT2G17030 28.158 380 205 16 10 351 5 354 2.68e-23 100
MS.gene23495.t1 AT2G17690 26.316 285 163 10 10 272 5 264 4.12e-15 76.6
MS.gene23495.t1 AT2G17036 24.242 396 233 16 12 361 6 380 4.28e-14 73.6
MS.gene23495.t1 AT2G17036 24.242 396 233 16 12 361 7 381 4.97e-14 73.2
MS.gene23495.t1 AT1G57906 28.279 244 122 12 110 326 11 228 2.56e-11 63.5

Find 57 sgRNAs with CRISPR-Local

Find 72 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ACTTTATGTTGTGGATAAAA+TGG 0.170692 7.3:+14582392 MS.gene23495:CDS
CATATCTTCCTGAAGAATTA+TGG 0.174102 7.3:+14581794 MS.gene23495:CDS
ACTTCTTCTTGTTCTAATAA+AGG 0.211089 7.3:+14582006 MS.gene23495:CDS
TCCACCATCATCCTTTAATA+TGG 0.252077 7.3:-14582328 None:intergenic
GGGTGATGTTTCGTTTGTTT+TGG 0.269127 7.3:+14582680 MS.gene23495:CDS
ATGGTTAGATGTAAAAGATT+TGG 0.281291 7.3:+14582659 MS.gene23495:CDS
TTTCAACTTGGAGAATTTAG+AGG 0.305854 7.3:+14582794 MS.gene23495:CDS
CTTCTTCTTGTTCTAATAAA+GGG 0.306699 7.3:+14582007 MS.gene23495:CDS
ATAATTTCCCATAATTCTTC+AGG 0.324466 7.3:-14581802 None:intergenic
TTGATCAAGAATGGGGTAAA+TGG 0.326226 7.3:+14582640 MS.gene23495:CDS
TTCATCAAATTCAACACTTT+AGG 0.328588 7.3:-14582126 None:intergenic
AGACTCAAACATTGCATTGT+TGG 0.341865 7.3:+14582707 MS.gene23495:CDS
TGGTTAGATGTAAAAGATTT+GGG 0.347718 7.3:+14582660 MS.gene23495:CDS
TGGTTGAATGCGGTATATTT+TGG 0.350450 7.3:-14581959 None:intergenic
CAATGCTTTGTCTTTGAAGT+TGG 0.363091 7.3:+14582431 MS.gene23495:CDS
TTAGAACAAGAAGAAGTTGA+TGG 0.363611 7.3:-14582000 None:intergenic
ATATCTTCCTGAAGAATTAT+GGG 0.363732 7.3:+14581795 MS.gene23495:CDS
TCCGCAGTACTTGTAGTTCA+TGG 0.380067 7.3:+14581863 MS.gene23495:CDS
CAGGACTTCTTGTTTCAACT+TGG 0.381282 7.3:+14582782 MS.gene23495:CDS
TTTATAAGCTTGATCAAGAA+TGG 0.392244 7.3:+14582631 MS.gene23495:CDS
GATGAATATGAATGACAAAA+AGG 0.393608 7.3:+14581759 None:intergenic
CTTTATGTTGTGGATAAAAT+GGG 0.396836 7.3:+14582393 MS.gene23495:CDS
AAGTTGAAAGTGTCTAATAT+TGG 0.417696 7.3:+14582291 MS.gene23495:CDS
TCGTGAGAGAGAGGTGGAGG+AGG 0.431799 7.3:-14581898 None:intergenic
TTATAAGCTTGATCAAGAAT+GGG 0.450966 7.3:+14582632 MS.gene23495:CDS
TTCTGTTGAGGGTCGTCGCA+TGG 0.464033 7.3:+14582245 MS.gene23495:CDS
TGATGATATAATACTTTACA+AGG 0.467606 7.3:+14582365 MS.gene23495:CDS
ATTTGTATATTGACGATGAA+AGG 0.475996 7.3:+14582565 MS.gene23495:CDS
ATCAAAGAGATAAAATTTGA+AGG 0.482053 7.3:-14582061 None:intergenic
CAAATTGGGTCACATATTGA+AGG 0.498468 7.3:+14582593 MS.gene23495:CDS
AGGTCGTGAGAGAGAGGTGG+AGG 0.524922 7.3:-14581901 None:intergenic
GACCTTTGCATTCCACATCA+AGG 0.529468 7.3:+14581919 MS.gene23495:CDS
CAAGGGACAACTTTATGTTG+TGG 0.530035 7.3:+14582383 MS.gene23495:CDS
GTGACAGGATGGAACAAAGT+TGG 0.530217 7.3:-14582846 None:intergenic
AATACCATATTAAAGGATGA+TGG 0.535242 7.3:+14582324 MS.gene23495:CDS
TTTGATGAACATTCGAGTTG+TGG 0.535981 7.3:+14582140 MS.gene23495:CDS
CATATTGAAGGCTATTTGTG+TGG 0.549161 7.3:+14582605 MS.gene23495:CDS
CTTCTTGTTCTAATAAAGGG+TGG 0.561460 7.3:+14582010 MS.gene23495:CDS
AAACAATTAGTAGAGTCTGA+TGG 0.567913 7.3:+14582525 MS.gene23495:CDS
GGTTCGTGATCATGCAATGA+TGG 0.577634 7.3:-14581979 None:intergenic
CAAGATTACTACGGATGTGA+AGG 0.582722 7.3:+14582732 MS.gene23495:CDS
TCTACTTCTATTGTGAAGAC+AGG 0.586419 7.3:+14582763 MS.gene23495:CDS
CAAATTAACTCGTGACAGGA+TGG 0.587347 7.3:-14582857 None:intergenic
ATCCTTGATGTGGAATGCAA+AGG 0.593156 7.3:-14581921 None:intergenic
CTTACAAATTAACTCGTGAC+AGG 0.596567 7.3:-14582861 None:intergenic
AGTAGAACAAATCCTTGATG+TGG 0.613692 7.3:-14581931 None:intergenic
ATGCAAAGGTCGTGAGAGAG+AGG 0.614624 7.3:-14581907 None:intergenic
CAAAGGTCGTGAGAGAGAGG+TGG 0.624486 7.3:-14581904 None:intergenic
TTGTTGGCTCAAGATTACTA+CGG 0.627281 7.3:+14582723 MS.gene23495:CDS
TATAAGCTTGATCAAGAATG+GGG 0.627945 7.3:+14582633 MS.gene23495:CDS
GATGATATAATACTTTACAA+GGG 0.643392 7.3:+14582366 MS.gene23495:CDS
ACCATATTAAAGGATGATGG+TGG 0.644107 7.3:+14582327 MS.gene23495:CDS
TAATAAGAGACTCCTATCGA+AGG 0.647154 7.3:+14582092 MS.gene23495:CDS
GGATGGAACAAAGTTGGACA+AGG 0.682403 7.3:-14582840 None:intergenic
GTCTGATGGAAATCTCTATG+TGG 0.689360 7.3:+14582539 MS.gene23495:CDS
GCCATGAACTACAAGTACTG+CGG 0.714603 7.3:-14581864 None:intergenic
CATGCAATGATGGTTGAATG+CGG 0.773749 7.3:-14581969 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TATGTCATAATTTTGATTTT+CGG + chr7.3:14582184-14582203 MS.gene23495:CDS 15.0%
!! ACAGAAAAATACCATATTAA+AGG + chr7.3:14582317-14582336 MS.gene23495:CDS 20.0%
!! ATCAAAGAGATAAAATTTGA+AGG - chr7.3:14582064-14582083 None:intergenic 20.0%
!! GATGATATAATACTTTACAA+GGG + chr7.3:14582366-14582385 MS.gene23495:CDS 20.0%
!! TGATGATATAATACTTTACA+AGG + chr7.3:14582365-14582384 MS.gene23495:CDS 20.0%
! AATACCATATTAAAGGATGA+TGG + chr7.3:14582324-14582343 MS.gene23495:CDS 25.0%
! ACTTCTTCTTGTTCTAATAA+AGG + chr7.3:14582006-14582025 MS.gene23495:CDS 25.0%
! ACTTTATGTTGTGGATAAAA+TGG + chr7.3:14582392-14582411 MS.gene23495:CDS 25.0%
! ATAATTTCCCATAATTCTTC+AGG - chr7.3:14581805-14581824 None:intergenic 25.0%
! ATATCTTCCTGAAGAATTAT+GGG + chr7.3:14581795-14581814 MS.gene23495:CDS 25.0%
! ATGGTTAGATGTAAAAGATT+TGG + chr7.3:14582659-14582678 MS.gene23495:CDS 25.0%
! ATTTGTATATTGACGATGAA+AGG + chr7.3:14582565-14582584 MS.gene23495:CDS 25.0%
! CTTCTTCTTGTTCTAATAAA+GGG + chr7.3:14582007-14582026 MS.gene23495:CDS 25.0%
! CTTTATGTTGTGGATAAAAT+GGG + chr7.3:14582393-14582412 MS.gene23495:CDS 25.0%
! TAAAAAGAGTAATATCTGGA+AGG + chr7.3:14582485-14582504 MS.gene23495:CDS 25.0%
! TGGTTAGATGTAAAAGATTT+GGG + chr7.3:14582660-14582679 MS.gene23495:CDS 25.0%
! TTATAAGCTTGATCAAGAAT+GGG + chr7.3:14582632-14582651 MS.gene23495:CDS 25.0%
! TTCATCAAATTCAACACTTT+AGG - chr7.3:14582129-14582148 None:intergenic 25.0%
! TTTATAAGCTTGATCAAGAA+TGG + chr7.3:14582631-14582650 MS.gene23495:CDS 25.0%
!! ATAAAATGGGAACAATTTTC+TGG + chr7.3:14582406-14582425 MS.gene23495:CDS 25.0%
!! GATGAAAGGTTTTACAAATT+GGG + chr7.3:14582579-14582598 MS.gene23495:CDS 25.0%
!!! AAGTTGAAAGTGTCTAATAT+TGG + chr7.3:14582291-14582310 MS.gene23495:CDS 25.0%
!!! TTACCACAACATAAGTTTTT+TGG - chr7.3:14582468-14582487 None:intergenic 25.0%
AAACAATTAGTAGAGTCTGA+TGG + chr7.3:14582525-14582544 MS.gene23495:CDS 30.0%
CATATCTTCCTGAAGAATTA+TGG + chr7.3:14581794-14581813 MS.gene23495:CDS 30.0%
GTGGTAAAAAGAGTAATATC+TGG + chr7.3:14582481-14582500 MS.gene23495:CDS 30.0%
TATAAGCTTGATCAAGAATG+GGG + chr7.3:14582633-14582652 MS.gene23495:CDS 30.0%
TCACCAAAAAACTTATGTTG+TGG + chr7.3:14582462-14582481 MS.gene23495:CDS 30.0%
TTAGAACAAGAAGAAGTTGA+TGG - chr7.3:14582003-14582022 None:intergenic 30.0%
TTTCAACTTGGAGAATTTAG+AGG + chr7.3:14582794-14582813 MS.gene23495:CDS 30.0%
! CGATGAAAGGTTTTACAAAT+TGG + chr7.3:14582578-14582597 MS.gene23495:CDS 30.0%
! GATTTTCGGTTAAAACATGT+TGG + chr7.3:14582198-14582217 MS.gene23495:CDS 30.0%
!!! AGTAATCTTGTTTTCTGTTG+AGG + chr7.3:14582233-14582252 MS.gene23495:CDS 30.0%
!!! GTAATCTTGTTTTCTGTTGA+GGG + chr7.3:14582234-14582253 MS.gene23495:CDS 30.0%
AGACTCAAACATTGCATTGT+TGG + chr7.3:14582707-14582726 MS.gene23495:CDS 35.0%
AGTAGAACAAATCCTTGATG+TGG - chr7.3:14581934-14581953 None:intergenic 35.0%
CAAATTGGGTCACATATTGA+AGG + chr7.3:14582593-14582612 MS.gene23495:CDS 35.0%
CATATTGAAGGCTATTTGTG+TGG + chr7.3:14582605-14582624 MS.gene23495:CDS 35.0%
CTTCTTGTTCTAATAAAGGG+TGG + chr7.3:14582010-14582029 MS.gene23495:CDS 35.0%
TAATAAGAGACTCCTATCGA+AGG + chr7.3:14582092-14582111 MS.gene23495:CDS 35.0%
TCCACCATCATCCTTTAATA+TGG - chr7.3:14582331-14582350 None:intergenic 35.0%
TCTACTTCTATTGTGAAGAC+AGG + chr7.3:14582763-14582782 MS.gene23495:CDS 35.0%
TTGATCAAGAATGGGGTAAA+TGG + chr7.3:14582640-14582659 MS.gene23495:CDS 35.0%
! ACCATATTAAAGGATGATGG+TGG + chr7.3:14582327-14582346 MS.gene23495:CDS 35.0%
! GTGGCTCATTAAAGTTTTTC+AGG + chr7.3:14582029-14582048 MS.gene23495:CDS 35.0%
! TGGTTGAATGCGGTATATTT+TGG - chr7.3:14581962-14581981 None:intergenic 35.0%
!! CAATGCTTTGTCTTTGAAGT+TGG + chr7.3:14582431-14582450 MS.gene23495:CDS 35.0%
!! GGTGATGTTTCGTTTGTTTT+GGG + chr7.3:14582681-14582700 MS.gene23495:CDS 35.0%
!! TTGTTGGCTCAAGATTACTA+CGG + chr7.3:14582723-14582742 MS.gene23495:CDS 35.0%
!! TTTGATGAACATTCGAGTTG+TGG + chr7.3:14582140-14582159 MS.gene23495:CDS 35.0%
!!! CTAAGCCAGCTAACATTTTT+TGG + chr7.3:14582817-14582836 MS.gene23495:CDS 35.0%
!!! GAATGCGGTATATTTTGGTT+TGG - chr7.3:14581957-14581976 None:intergenic 35.0%
!!! GTGTAGTTTTATCCTTCGAT+AGG - chr7.3:14582107-14582126 None:intergenic 35.0%
ATCCTTGATGTGGAATGCAA+AGG - chr7.3:14581924-14581943 None:intergenic 40.0%
CAAGATTACTACGGATGTGA+AGG + chr7.3:14582732-14582751 MS.gene23495:CDS 40.0%
CAAGGCCAAAAAATGTTAGC+TGG - chr7.3:14582825-14582844 None:intergenic 40.0%
CAAGGGACAACTTTATGTTG+TGG + chr7.3:14582383-14582402 MS.gene23495:CDS 40.0%
CATGCAATGATGGTTGAATG+CGG - chr7.3:14581972-14581991 None:intergenic 40.0%
GTCTGATGGAAATCTCTATG+TGG + chr7.3:14582539-14582558 MS.gene23495:CDS 40.0%
! CAGGACTTCTTGTTTCAACT+TGG + chr7.3:14582782-14582801 MS.gene23495:CDS 40.0%
! GGGTGATGTTTCGTTTGTTT+TGG + chr7.3:14582680-14582699 MS.gene23495:CDS 40.0%
GACCTTTGCATTCCACATCA+AGG + chr7.3:14581919-14581938 MS.gene23495:CDS 45.0%
GGATGGAACAAAGTTGGACA+AGG - chr7.3:14582843-14582862 None:intergenic 45.0%
GGTTCGTGATCATGCAATGA+TGG - chr7.3:14581982-14582001 None:intergenic 45.0%
GTGACAGGATGGAACAAAGT+TGG - chr7.3:14582849-14582868 None:intergenic 45.0%
TCCGCAGTACTTGTAGTTCA+TGG + chr7.3:14581863-14581882 MS.gene23495:CDS 45.0%
! GCCATGAACTACAAGTACTG+CGG - chr7.3:14581867-14581886 None:intergenic 45.0%
ATGCAAAGGTCGTGAGAGAG+AGG - chr7.3:14581910-14581929 None:intergenic 50.0%
CAAAGGTCGTGAGAGAGAGG+TGG - chr7.3:14581907-14581926 None:intergenic 55.0%
TTCTGTTGAGGGTCGTCGCA+TGG + chr7.3:14582245-14582264 MS.gene23495:CDS 55.0%
AGGTCGTGAGAGAGAGGTGG+AGG - chr7.3:14581904-14581923 None:intergenic 60.0%
TCGTGAGAGAGAGGTGGAGG+AGG - chr7.3:14581901-14581920 None:intergenic 60.0%


Chromosome Type Strat End Strand Name
chr7.3 gene 14581766 14582875 14581766 ID=MS.gene23495
chr7.3 mRNA 14581766 14582875 14581766 ID=MS.gene23495.t1;Parent=MS.gene23495
chr7.3 exon 14581766 14582875 14581766 ID=MS.gene23495.t1.exon1;Parent=MS.gene23495.t1
chr7.3 CDS 14581766 14582875 14581766 ID=cds.MS.gene23495.t1;Parent=MS.gene23495.t1
Gene Sequence

>MS.gene23495

ATGAATGACAAAAAGGACTGTTCAAACTCATATCTTCCTGAAGAATTATGGGAAATTATATTAAAAAAACTGAAAACTACACTTGATGTTGTTCGATTCCGCAGTACTTGTAGTTCATGGCACTCTTTTCTTCCTCCTCCACCTCTCTCTCACGACCTTTGCATTCCACATCAAGGATTTGTTCTACTCCAAACCAAAATATACCGCATTCAACCATCATTGCATGATCACGAACCATCAACTTCTTCTTGTTCTAATAAAGGGTGGCTCATTAAAGTTTTTCAGGATTCAAATTCCTTCAAATTTTATCTCTTTGATCTTGTCACTAATAAGAGACTCCTATCGAAGGATAAAACTACACCTAAAGTGTTGAATTTGATGAACATTCGAGTTGTGGAGTTGTTTGAACTATACACTCTATGTCATAATTTTGATTTTCGGTTAAAACATGTTGGTAATGTTCGCAAAGTAATCTTGTTTTCTGTTGAGGGTCGTCGCATGGTCTTTGCATTATATACTGATAAAAAGTTGAAAGTGTCTAATATTGGAGAACAGAAAAATACCATATTAAAGGATGATGGTGGAGAAAACAAGTATTTTGATGATATAATACTTTACAAGGGACAACTTTATGTTGTGGATAAAATGGGAACAATTTTCTGGATCAATGCTTTGTCTTTGAAGTTGGTTCAATTTTCACCAAAAAACTTATGTTGTGGTAAAAAGAGTAATATCTGGAAGGTTTCTAGTACATACACTAAACAATTAGTAGAGTCTGATGGAAATCTCTATGTGGTTGATTTGTATATTGACGATGAAAGGTTTTACAAATTGGGTCACATATTGAAGGCTATTTGTGTGGAAGTTTATAAGCTTGATCAAGAATGGGGTAAATGGTTAGATGTAAAAGATTTGGGTGATGTTTCGTTTGTTTTGGGTAAAGACTCAAACATTGCATTGTTGGCTCAAGATTACTACGGATGTGAAGGAAATTGCATCTACTTCTATTGTGAAGACAGGACTTCTTGTTTCAACTTGGAGAATTTAGAGGCTAAGCCAGCTAACATTTTTTGGCCTTGTCCAACTTTGTTCCATCCTGTCACGAGTTAA

Protein sequence

>MS.gene23495.t1

MNDKKDCSNSYLPEELWEIILKKLKTTLDVVRFRSTCSSWHSFLPPPPLSHDLCIPHQGFVLLQTKIYRIQPSLHDHEPSTSSCSNKGWLIKVFQDSNSFKFYLFDLVTNKRLLSKDKTTPKVLNLMNIRVVELFELYTLCHNFDFRLKHVGNVRKVILFSVEGRRMVFALYTDKKLKVSNIGEQKNTILKDDGGENKYFDDIILYKGQLYVVDKMGTIFWINALSLKLVQFSPKNLCCGKKSNIWKVSSTYTKQLVESDGNLYVVDLYIDDERFYKLGHILKAICVEVYKLDQEWGKWLDVKDLGDVSFVLGKDSNIALLAQDYYGCEGNCIYFYCEDRTSCFNLENLEAKPANIFWPCPTLFHPVTS