AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene30094


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene30094.t1 MTR_7g096720 73.740 377 91 3 1 371 6 380 0.0 552
MS.gene30094.t1 MTR_4g088925 65.922 358 112 4 16 373 34 381 2.54e-151 432
MS.gene30094.t1 MTR_8g085760 54.400 375 143 11 11 368 16 379 1.35e-118 349
MS.gene30094.t1 MTR_6g092570 44.658 365 169 13 11 369 11 348 1.05e-71 228
MS.gene30094.t1 MTR_5g079830 44.318 352 161 14 11 353 9 334 2.64e-69 223
MS.gene30094.t1 MTR_8g074610 35.097 359 193 11 15 351 32 372 7.07e-54 183
MS.gene30094.t1 MTR_2g041510 37.566 378 178 15 1 351 1 347 2.31e-53 181
MS.gene30094.t1 MTR_2g041510 37.566 378 178 15 1 351 21 367 4.08e-53 181
MS.gene30094.t1 MTR_8g074640 33.333 381 197 14 1 352 1 353 3.81e-49 170
MS.gene30094.t1 MTR_4g125090 37.931 319 135 14 11 319 48 313 3.30e-44 155
MS.gene30094.t1 MTR_7g096760 70.213 94 26 2 218 310 1 93 3.12e-35 126
MS.gene30094.t1 MTR_3g030050 24.742 388 203 12 12 340 20 377 2.13e-19 89.4
MS.gene30094.t1 MTR_1g026850 24.642 349 210 12 12 341 10 324 2.42e-16 80.1
MS.gene30094.t1 MTR_3g058460 22.222 342 228 9 12 341 19 334 7.21e-15 75.5
MS.gene30094.t1 MTR_3g029650 24.789 355 190 13 12 338 9 314 3.24e-14 74.7
MS.gene30094.t1 MTR_6g018400 23.636 330 196 10 28 336 26 320 1.36e-13 71.6
MS.gene30094.t1 MTR_3g029800 23.200 375 221 12 11 359 7 340 8.50e-13 69.3
MS.gene30094.t1 MTR_3g030380 23.343 347 213 10 11 342 8 316 1.70e-12 68.6
MS.gene30094.t1 MTR_3g029950 23.446 354 205 10 12 342 11 321 4.84e-11 63.9
MS.gene30094.t1 MTR_3g029620 22.195 401 211 14 11 359 6 357 5.04e-11 63.9
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene30094.t1 AT1G44080 31.054 351 193 9 11 351 8 319 4.32e-39 142
MS.gene30094.t1 AT1G65770 28.375 363 204 11 19 359 14 342 7.50e-38 139
MS.gene30094.t1 AT2G26160 29.217 332 210 7 23 350 20 330 1.51e-37 138
MS.gene30094.t1 AT4G35733 30.055 366 217 13 11 363 9 348 7.01e-31 120
MS.gene30094.t1 AT3G25750 28.150 373 213 13 11 369 8 339 7.51e-31 120
MS.gene30094.t1 AT3G25750 28.150 373 227 12 11 369 8 353 5.92e-30 118
MS.gene30094.t1 AT1G65740 28.763 372 215 13 11 357 6 352 3.18e-28 114
MS.gene30094.t1 AT1G65760 28.857 350 205 9 5 338 2 323 2.01e-24 103
MS.gene30094.t1 AT2G17030 26.171 363 207 15 11 338 6 342 2.40e-20 92.0
MS.gene30094.t1 AT2G17036 24.863 366 217 14 11 344 5 344 4.15e-15 76.6
MS.gene30094.t1 AT2G17036 24.863 366 217 14 11 344 6 345 4.28e-15 76.6

Find 64 sgRNAs with CRISPR-Local

Find 71 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ACTTTATGTTGTAGATAATA+TGG 0.178448 3.2:+42375176 MS.gene30094:CDS
CACAATTCTTCAAGAAGATT+TGG 0.198657 3.2:-42374571 None:intergenic
TCCGCAGTATTTGTAGTTTA+TGG 0.204499 3.2:+42374641 MS.gene30094:CDS
AGTCGTTATGATGATAAAAT+TGG 0.239302 3.2:+42374961 MS.gene30094:CDS
GACATCCAAGAGATGGAATT+TGG 0.248210 3.2:-42374842 None:intergenic
CATTATACTTACAAATTAAC+TGG 0.259230 3.2:-42375658 None:intergenic
ACTTCTTCTTGTTCTAATAA+AGG 0.268247 3.2:+42374784 MS.gene30094:CDS
ATGATGATAAAATTGGTTCT+AGG 0.292102 3.2:+42374968 MS.gene30094:CDS
TGCACTATTGTGATGAAAAT+AGG 0.305463 3.2:+42375259 MS.gene30094:CDS
TGGTTGAATGCTGTATATTT+TGG 0.321312 3.2:-42374737 None:intergenic
TTGATCAAGAATGGGGTAAA+TGG 0.326226 3.2:+42375427 MS.gene30094:CDS
ATGGTTAGAAGTAAAAGATT+TGG 0.332561 3.2:+42375446 MS.gene30094:CDS
ATCCTCCAAATTCCATCTCT+TGG 0.362838 3.2:+42374837 MS.gene30094:CDS
ACAATTCTTCAAGAAGATTT+GGG 0.367616 3.2:-42374570 None:intergenic
TGGTTAGAAGTAAAAGATTT+GGG 0.374795 3.2:+42375447 MS.gene30094:CDS
TTTCAGTAGTTTCCTCAATT+CGG 0.377113 3.2:-42374882 None:intergenic
TTCTAGTGAGGGTCGTTGTA+TGG 0.378472 3.2:+42375029 MS.gene30094:CDS
CAAAAGGGGTTACTTCTTGA+AGG 0.384442 3.2:+42375380 MS.gene30094:CDS
GACAAGAAAATGTTGTCAAC+AGG 0.389342 3.2:-42375609 None:intergenic
TTTATAAGCTTGATCAAGAA+TGG 0.392244 3.2:+42375418 MS.gene30094:CDS
TTGTGAGGGGTAGGTGGAGG+AGG 0.399257 3.2:-42374676 None:intergenic
ATGATGAAAGGTATTACAAA+AGG 0.404208 3.2:+42375364 MS.gene30094:CDS
CTTCTTGTTCTAATAAAGGC+TGG 0.414040 3.2:+42374788 MS.gene30094:CDS
CAATGCTTTGTCCTTGAAGT+TGG 0.422760 3.2:+42375215 MS.gene30094:CDS
CAAATCTTCTTGAAGAATTG+TGG 0.426488 3.2:+42374572 MS.gene30094:CDS
TGATGAAAGGTATTACAAAA+GGG 0.432135 3.2:+42375365 MS.gene30094:CDS
CTTTATGTTGTAGATAATAT+GGG 0.438590 3.2:+42375177 MS.gene30094:CDS
TGATGATAAAATTGGTTCTA+GGG 0.442982 3.2:+42374969 MS.gene30094:CDS
TTATAAGCTTGATCAAGAAT+GGG 0.450966 3.2:+42375419 MS.gene30094:CDS
GTTATATATGCTAATTATGT+TGG 0.461457 3.2:+42374991 MS.gene30094:CDS
AAATCTTCTTGAAGAATTGT+GGG 0.471775 3.2:+42374573 MS.gene30094:CDS
AAAGAAACATATCAAAATAG+AGG 0.476420 3.2:+42375101 MS.gene30094:CDS
GTTGTAGAAATGCCACAATC+TGG 0.476536 3.2:-42374763 None:intergenic
TGAAAACTGAACCAACTTCA+AGG 0.479490 3.2:-42375226 None:intergenic
GAGTTGATTGTTTCCAACAT+TGG 0.484992 3.2:+42375075 MS.gene30094:CDS
ATGTTGTGAGGGGTAGGTGG+AGG 0.491653 3.2:-42374679 None:intergenic
TGACGATATAATACATTACA+AGG 0.493104 3.2:+42375149 MS.gene30094:CDS
ATTCTTCAAGTTGAAACAAG+AGG 0.499533 3.2:-42375574 None:intergenic
TTTGATGAACTTTCGAGTTG+TGG 0.503494 3.2:+42374915 MS.gene30094:CDS
CAAATGTTGTGAGGGGTAGG+TGG 0.529285 3.2:-42374682 None:intergenic
AAGCAATTAGTAGAGTATGA+TGG 0.529382 3.2:+42375312 MS.gene30094:CDS
GCTACATGATGAAACAAAGT+TGG 0.533530 3.2:-42375636 None:intergenic
GTGATGAAAATAGGCATACA+AGG 0.549885 3.2:+42375268 MS.gene30094:CDS
TAATAAGAGACTCCGAATTG+AGG 0.556125 3.2:+42374870 MS.gene30094:CDS
TAGTGAAGACATCCAAGAGA+TGG 0.557700 3.2:-42374849 None:intergenic
GATGAAAGGTATTACAAAAG+GGG 0.559891 3.2:+42375366 MS.gene30094:CDS
CATATCAAAATAGAGGATGA+TGG 0.568179 3.2:+42375108 MS.gene30094:CDS
CATGAATATGAATACGAAAG+TGG 0.569457 3.2:+42374537 None:intergenic
CAAGATTATGAAGGATGTGA+AGG 0.569990 3.2:+42375519 MS.gene30094:CDS
AAGCAAATGTTGTGAGGGGT+AGG 0.570555 3.2:-42374685 None:intergenic
ATCCAAGAGATGGAATTTGG+AGG 0.589010 3.2:-42374839 None:intergenic
ATATGTATATTAATGATGAA+AGG 0.590203 3.2:+42375352 MS.gene30094:CDS
AGTGAAAGCAAATGTTGTGA+GGG 0.605960 3.2:-42374690 None:intergenic
TTATTGGCTCAAGATTATGA+AGG 0.613986 3.2:+42375510 MS.gene30094:CDS
GACGATATAATACATTACAA+GGG 0.615067 3.2:+42375150 MS.gene30094:CDS
ATTCAACCATCACCAGATTG+TGG 0.624188 3.2:+42374751 MS.gene30094:CDS
TATAAGCTTGATCAAGAATG+GGG 0.627945 3.2:+42375420 MS.gene30094:CDS
GTGAAAGCAAATGTTGTGAG+GGG 0.651949 3.2:-42374689 None:intergenic
GAAATGCCACAATCTGGTGA+TGG 0.665746 3.2:-42374757 None:intergenic
ATCAAAATAGAGGATGATGG+TGG 0.667308 3.2:+42375111 MS.gene30094:CDS
TGATGAAAATAGGCATACAA+GGG 0.680906 3.2:+42375269 MS.gene30094:CDS
TAGTGAAAGCAAATGTTGTG+AGG 0.685541 3.2:-42374691 None:intergenic
GCCATAAACTACAAATACTG+CGG 0.690280 3.2:-42374642 None:intergenic
GTATGATGGAAGTCTATATG+TGG 0.691573 3.2:+42375326 MS.gene30094:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATATGTATATTAATGATGAA+AGG + chr3.2:42375352-42375371 MS.gene30094:CDS 15.0%
!! AAAGAAACATATCAAAATAG+AGG + chr3.2:42375101-42375120 MS.gene30094:CDS 20.0%
!! ACTTTATGTTGTAGATAATA+TGG + chr3.2:42375176-42375195 MS.gene30094:CDS 20.0%
!! CTTTATGTTGTAGATAATAT+GGG + chr3.2:42375177-42375196 MS.gene30094:CDS 20.0%
!! GTTATATATGCTAATTATGT+TGG + chr3.2:42374991-42375010 MS.gene30094:CDS 20.0%
! AAATCTTCTTGAAGAATTGT+GGG + chr3.2:42374573-42374592 MS.gene30094:CDS 25.0%
! ACAATTCTTCAAGAAGATTT+GGG - chr3.2:42374573-42374592 None:intergenic 25.0%
! ACTTCTTCTTGTTCTAATAA+AGG + chr3.2:42374784-42374803 MS.gene30094:CDS 25.0%
! AGTCGTTATGATGATAAAAT+TGG + chr3.2:42374961-42374980 MS.gene30094:CDS 25.0%
! ATGGTTAGAAGTAAAAGATT+TGG + chr3.2:42375446-42375465 MS.gene30094:CDS 25.0%
! GACGATATAATACATTACAA+GGG + chr3.2:42375150-42375169 MS.gene30094:CDS 25.0%
! TGACGATATAATACATTACA+AGG + chr3.2:42375149-42375168 MS.gene30094:CDS 25.0%
! TGGTTAGAAGTAAAAGATTT+GGG + chr3.2:42375447-42375466 MS.gene30094:CDS 25.0%
! TTATAAGCTTGATCAAGAAT+GGG + chr3.2:42375419-42375438 MS.gene30094:CDS 25.0%
! TTTATAAGCTTGATCAAGAA+TGG + chr3.2:42375418-42375437 MS.gene30094:CDS 25.0%
!! AGACTCAAATTTTGCATTAT+TGG + chr3.2:42375494-42375513 MS.gene30094:CDS 25.0%
!! ATAATATGGGAACAATTTTC+TGG + chr3.2:42375190-42375209 MS.gene30094:CDS 25.0%
!! ATGATGATAAAATTGGTTCT+AGG + chr3.2:42374968-42374987 MS.gene30094:CDS 25.0%
!!! ATGATGAAAGGTATTACAAA+AGG + chr3.2:42375364-42375383 MS.gene30094:CDS 25.0%
!!! TGATGAAAGGTATTACAAAA+GGG + chr3.2:42375365-42375384 MS.gene30094:CDS 25.0%
!!! TGATGATAAAATTGGTTCTA+GGG + chr3.2:42374969-42374988 MS.gene30094:CDS 25.0%
AAGCAATTAGTAGAGTATGA+TGG + chr3.2:42375312-42375331 MS.gene30094:CDS 30.0%
CAAATCTTCTTGAAGAATTG+TGG + chr3.2:42374572-42374591 MS.gene30094:CDS 30.0%
CACAATTCTTCAAGAAGATT+TGG - chr3.2:42374574-42374593 None:intergenic 30.0%
CATATCAAAATAGAGGATGA+TGG + chr3.2:42375108-42375127 MS.gene30094:CDS 30.0%
TATAAGCTTGATCAAGAATG+GGG + chr3.2:42375420-42375439 MS.gene30094:CDS 30.0%
TGATGAAAATAGGCATACAA+GGG + chr3.2:42375269-42375288 MS.gene30094:CDS 30.0%
TGCACTATTGTGATGAAAAT+AGG + chr3.2:42375259-42375278 MS.gene30094:CDS 30.0%
TTTCAGTAGTTTCCTCAATT+CGG - chr3.2:42374885-42374904 None:intergenic 30.0%
! ATGTTTCTTTTCTCCAATGT+TGG - chr3.2:42375091-42375110 None:intergenic 30.0%
! TGGTTGAATGCTGTATATTT+TGG - chr3.2:42374740-42374759 None:intergenic 30.0%
!! ATTCTTCAAGTTGAAACAAG+AGG - chr3.2:42375577-42375596 None:intergenic 30.0%
!! GATGAAAGGTATTACAAAAG+GGG + chr3.2:42375366-42375385 MS.gene30094:CDS 30.0%
!! TTATTGGCTCAAGATTATGA+AGG + chr3.2:42375510-42375529 MS.gene30094:CDS 30.0%
!!! AGTAATCTTGTTTTCTAGTG+AGG + chr3.2:42375017-42375036 MS.gene30094:CDS 30.0%
!!! GTAATCTTGTTTTCTAGTGA+GGG + chr3.2:42375018-42375037 MS.gene30094:CDS 30.0%
!!! TCACAATAGTGCAAGTTTTT+TGG - chr3.2:42375252-42375271 None:intergenic 30.0%
AGTGAAAGCAAATGTTGTGA+GGG - chr3.2:42374693-42374712 None:intergenic 35.0%
CAAGATTATGAAGGATGTGA+AGG + chr3.2:42375519-42375538 MS.gene30094:CDS 35.0%
CTTCTTGTTCTAATAAAGGC+TGG + chr3.2:42374788-42374807 MS.gene30094:CDS 35.0%
GACAAGAAAATGTTGTCAAC+AGG - chr3.2:42375612-42375631 None:intergenic 35.0%
GCCATAAACTACAAATACTG+CGG - chr3.2:42374645-42374664 None:intergenic 35.0%
GCTACATGATGAAACAAAGT+TGG - chr3.2:42375639-42375658 None:intergenic 35.0%
GTGATGAAAATAGGCATACA+AGG + chr3.2:42375268-42375287 MS.gene30094:CDS 35.0%
TAATAAGAGACTCCGAATTG+AGG + chr3.2:42374870-42374889 MS.gene30094:CDS 35.0%
TAGTGAAAGCAAATGTTGTG+AGG - chr3.2:42374694-42374713 None:intergenic 35.0%
TCCGCAGTATTTGTAGTTTA+TGG + chr3.2:42374641-42374660 MS.gene30094:CDS 35.0%
TGAAAACTGAACCAACTTCA+AGG - chr3.2:42375229-42375248 None:intergenic 35.0%
TTGATCAAGAATGGGGTAAA+TGG + chr3.2:42375427-42375446 MS.gene30094:CDS 35.0%
! ATCAAAATAGAGGATGATGG+TGG + chr3.2:42375111-42375130 MS.gene30094:CDS 35.0%
! GAGTTGATTGTTTCCAACAT+TGG + chr3.2:42375075-42375094 MS.gene30094:CDS 35.0%
! GTATGATGGAAGTCTATATG+TGG + chr3.2:42375326-42375345 MS.gene30094:CDS 35.0%
!! TTTGATGAACTTTCGAGTTG+TGG + chr3.2:42374915-42374934 MS.gene30094:CDS 35.0%
!!! GGTGATGCTTCTTTTGTTTT+GGG + chr3.2:42375468-42375487 MS.gene30094:CDS 35.0%
ATCCAAGAGATGGAATTTGG+AGG - chr3.2:42374842-42374861 None:intergenic 40.0%
ATCCTCCAAATTCCATCTCT+TGG + chr3.2:42374837-42374856 MS.gene30094:CDS 40.0%
ATTCAACCATCACCAGATTG+TGG + chr3.2:42374751-42374770 MS.gene30094:CDS 40.0%
CAAAAGGGGTTACTTCTTGA+AGG + chr3.2:42375380-42375399 MS.gene30094:CDS 40.0%
CAATGCTTTGTCCTTGAAGT+TGG + chr3.2:42375215-42375234 MS.gene30094:CDS 40.0%
GACATCCAAGAGATGGAATT+TGG - chr3.2:42374845-42374864 None:intergenic 40.0%
GTGAAAGCAAATGTTGTGAG+GGG - chr3.2:42374692-42374711 None:intergenic 40.0%
GTTGTAGAAATGCCACAATC+TGG - chr3.2:42374766-42374785 None:intergenic 40.0%
TAGTGAAGACATCCAAGAGA+TGG - chr3.2:42374852-42374871 None:intergenic 40.0%
!! CTTCTTGAAGGCTGTTTTAG+TGG + chr3.2:42375392-42375411 MS.gene30094:CDS 40.0%
!! GGGTGATGCTTCTTTTGTTT+TGG + chr3.2:42375467-42375486 MS.gene30094:CDS 40.0%
AAGCAAATGTTGTGAGGGGT+AGG - chr3.2:42374688-42374707 None:intergenic 45.0%
GAAATGCCACAATCTGGTGA+TGG - chr3.2:42374760-42374779 None:intergenic 45.0%
TTCTAGTGAGGGTCGTTGTA+TGG + chr3.2:42375029-42375048 MS.gene30094:CDS 45.0%
CAAATGTTGTGAGGGGTAGG+TGG - chr3.2:42374685-42374704 None:intergenic 50.0%
ATGTTGTGAGGGGTAGGTGG+AGG - chr3.2:42374682-42374701 None:intergenic 55.0%
TTGTGAGGGGTAGGTGGAGG+AGG - chr3.2:42374679-42374698 None:intergenic 60.0%


Chromosome Type Strat End Strand Name
chr3.2 gene 42374544 42375665 42374544 ID=MS.gene30094
chr3.2 mRNA 42374544 42375665 42374544 ID=MS.gene30094.t1;Parent=MS.gene30094
chr3.2 exon 42374544 42375665 42374544 ID=MS.gene30094.t1.exon1;Parent=MS.gene30094.t1
chr3.2 CDS 42374544 42375665 42374544 ID=cds.MS.gene30094.t1;Parent=MS.gene30094.t1
Gene Sequence

>MS.gene30094

ATGAATACGAAAGTGGAGTGTTCAAACCCAAATCTTCTTGAAGAATTGTGGGTGATTATATTCAAAAAACTGAACACTACAGTTAGCGTTGTTCGATTCCGCAGTATTTGTAGTTTATGGCGCTCTCTTCTTCCTCCTCCACCTACCCCTCACAACATTTGCTTTCACTATCAAAATTTATTTCTACTTCAAACCAAAATATACAGCATTCAACCATCACCAGATTGTGGCATTTCTACAACTTCTTCTTGTTCTAATAAAGGCTGGATCATTAAAGTTTTTCAGAATTCAAAATCCTCCAAATTCCATCTCTTGGATGTCTTCACTAATAAGAGACTCCGAATTGAGGAAACTACTGAAAAAGTTTTGAATTTGATGAACTTTCGAGTTGTGGAATTGTTTGAACAATATACTCAAAGTCGTTATGATGATAAAATTGGTTCTAGGGTTATATATGCTAATTATGTTGGCAAAGTAATCTTGTTTTCTAGTGAGGGTCGTTGTATGGTCTTTGTACTGAATAATGATAAAGAGTTGATTGTTTCCAACATTGGAGAAAAGAAACATATCAAAATAGAGGATGATGGTGGAGAAAACAATTATTTTGACGATATAATACATTACAAGGGACAACTTTATGTTGTAGATAATATGGGAACAATTTTCTGGATCAATGCTTTGTCCTTGAAGTTGGTTCAGTTTTCACCAAAAAACTTGCACTATTGTGATGAAAATAGGCATACAAGGGTGAAATCTAATATAAACAAGAAGCAATTAGTAGAGTATGATGGAAGTCTATATGTGGTTGATATGTATATTAATGATGAAAGGTATTACAAAAGGGGTTACTTCTTGAAGGCTGTTTTAGTGGAAGTTTATAAGCTTGATCAAGAATGGGGTAAATGGTTAGAAGTAAAAGATTTGGGTGATGCTTCTTTTGTTTTGGGTAAAGACTCAAATTTTGCATTATTGGCTCAAGATTATGAAGGATGTGAAGGTAATTGCATCTACTTCTATAATGAAAGCAAAGCCTCTTGTTTCAACTTGAAGAATTCAGAGAGCAAACCTGTTGACAACATTTTCTTGTCATCTCCAACTTTGTTTCATCATGTAGCCAGTTAA

Protein sequence

>MS.gene30094.t1

MNTKVECSNPNLLEELWVIIFKKLNTTVSVVRFRSICSLWRSLLPPPPTPHNICFHYQNLFLLQTKIYSIQPSPDCGISTTSSCSNKGWIIKVFQNSKSSKFHLLDVFTNKRLRIEETTEKVLNLMNFRVVELFEQYTQSRYDDKIGSRVIYANYVGKVILFSSEGRCMVFVLNNDKELIVSNIGEKKHIKIEDDGGENNYFDDIIHYKGQLYVVDNMGTIFWINALSLKLVQFSPKNLHYCDENRHTRVKSNINKKQLVEYDGSLYVVDMYINDERYYKRGYFLKAVLVEVYKLDQEWGKWLEVKDLGDASFVLGKDSNFALLAQDYEGCEGNCIYFYNESKASCFNLKNSESKPVDNIFLSSPTLFHHVAS