AlfalfaGEDB | MS.gene022366

M. sativa cultivar XinJiangDaYe / MS.gene022366

KEGG: K02358
GO: GO:0000166 GO:0001882 GO:0001883 GO:0003674 GO:0003676 GO:0003723 GO:0003746 GO:0003824 GO:0003924 GO:0005488 GO:0005525 GO:0006414 GO:0008135 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009987 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0017076 GO:0017111 GO:0019001 GO:0032549 GO:0032550 GO:0032553 GO:0032555 GO:0032561 GO:0034645 GO:0035639 GO:0036094 GO:0043167 GO:0043168 GO:0043170 GO:0044237 GO:0044249 GO:0044260 GO:0045182 GO:0071704 GO:0090079 GO:0097159 GO:0097367 GO:1901265 GO:1901363 GO:1901576
PFAM: PF00009.30 PF03143.20 PF03144.28

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene022366.t1	XP_003601160.1	99.3	448	3	0	40	487	42	489	1.60E-249	871.7

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene022366.t1	O24310	95.3	448	21	0	40	487	41	488	2.1e-242	839.3

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene022366.t1	G7J486	99.3	448	3	0	40	487	42	489	1.2e-249	871.7

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR

PPI:

Gene1	Gene2	Type
MS.gene022366	MS.gene47051	PPI
MS.gene022366	MS.gene52738	PPI
MS.gene022366	MS.gene005994	PPI
MS.gene022366	MS.gene018062	PPI
MS.gene022366	MS.gene34008	PPI
MS.gene022366	MS.gene041766	PPI
MS.gene022366	MS.gene54763	PPI
MS.gene022366	MS.gene005996	PPI
MS.gene022366	MS.gene058469	PPI
MS.gene47051	MS.gene022366	PPI
MS.gene022366	MS.gene006000	PPI
MS.gene022366	MS.gene72917	PPI
MS.gene022366	MS.gene074184	PPI
MS.gene022366	MS.gene54761	PPI
MS.gene022366	MS.gene041769	PPI
MS.gene022366	MS.gene34006	PPI
MS.gene022366	MS.gene04696	PPI
MS.gene022366	MS.gene074183	PPI
MS.gene022366	MS.gene016105	PPI
MS.gene022366	MS.gene001098	PPI
MS.gene022366	MS.gene041771	PPI
MS.gene022366	MS.gene001097	PPI
MS.gene022366	MS.gene47052	PPI
MS.gene022366	MS.gene33103	PPI

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene022366.t1	MTR_3g076660	98.160	489	7	1	1	487	1	489	0.0	974
MS.gene022366.t1	MTR_2g021300	60.137	439	156	4	52	486	12	435	0.0	524
MS.gene022366.t1	MTR_7g094460	61.075	465	157	7	27	486	3	448	3.47e-180	512
MS.gene022366.t1	MTR_3g072130	44.939	247	94	7	245	486	101	310	8.33e-52	178
MS.gene022366.t1	MTR_6g021800	30.787	445	245	16	86	485	3	429	8.89e-46	166
MS.gene022366.t1	MTR_6g021805	30.787	445	245	16	86	485	3	429	8.89e-46	166
MS.gene022366.t1	MTR_1g495705	30.112	445	248	16	86	485	3	429	1.46e-44	162
MS.gene022366.t1	MTR_1g095650	30.112	445	248	16	86	485	3	429	1.46e-44	162
MS.gene022366.t1	MTR_1g095703	29.888	445	249	16	86	485	3	429	2.28e-44	162
MS.gene022366.t1	MTR_4g014810	29.663	445	250	16	86	485	3	429	2.77e-43	159
MS.gene022366.t1	MTR_8g087950	29.663	445	248	16	90	487	275	701	9.39e-42	158
MS.gene022366.t1	MTR_8g087950	29.663	445	248	16	90	487	348	774	1.11e-41	159
MS.gene022366.t1	MTR_1g013680	29.083	447	253	15	86	485	3	432	6.24e-41	153
MS.gene022366.t1	MTR_1g085410	30.222	450	235	19	86	482	3	426	8.66e-38	144
MS.gene022366.t1	MTR_1g101870	30.562	445	241	20	86	485	3	424	2.13e-35	140
MS.gene022366.t1	MTR_1g101870	30.512	449	236	22	86	485	3	424	2.97e-35	140
MS.gene022366.t1	MTR_1g024175	28.442	443	252	15	86	482	3	426	4.08e-35	136
MS.gene022366.t1	MTR_8g014590	26.906	446	265	14	85	485	2	431	1.78e-32	129
MS.gene022366.t1	MTR_6g080420	27.009	448	258	13	87	485	88	515	2.38e-32	129
MS.gene022366.t1	MTR_2g082730	27.739	429	256	15	88	482	5	413	2.64e-30	124
MS.gene022366.t1	MTR_8g106700	26.966	445	262	13	87	485	43	470	3.96e-30	122
MS.gene022366.t1	MTR_8g106700	26.966	445	262	13	87	485	49	476	5.97e-30	122
MS.gene022366.t1	MTR_8g008740	30.000	330	185	11	80	380	2	314	7.93e-30	122
MS.gene022366.t1	MTR_8g106700	26.966	445	262	13	87	485	70	497	1.24e-29	121
MS.gene022366.t1	MTR_1g089520	27.727	440	241	20	87	482	5	411	2.86e-25	109
MS.gene022366.t1	MTR_5g037350	25.780	481	258	16	84	482	10	473	4.41e-25	108
MS.gene022366.t1	MTR_1g095650	29.191	346	197	15	170	485	1	328	1.85e-24	104
MS.gene022366.t1	MTR_2g082665	27.273	429	232	18	88	482	5	387	2.31e-23	103
MS.gene022366.t1	MTR_2g080210	24.485	437	247	13	88	485	7	399	6.87e-22	98.2
MS.gene022366.t1	MTR_4g079360	30.830	253	161	7	92	341	59	300	1.21e-21	99.0
MS.gene022366.t1	MTR_2g037420	25.926	432	240	15	88	485	5	390	1.09e-20	95.1
MS.gene022366.t1	MTR_1g101880	31.646	158	80	3	84	216	1	155	1.14e-17	83.2
MS.gene022366.t1	MTR_1g024165	33.557	149	76	3	80	206	2	149	5.92e-17	78.2
MS.gene022366.t1	MTR_8g013910	25.220	341	188	11	84	384	1	314	4.42e-15	77.8
MS.gene022366.t1	MTR_2g075870	26.690	281	151	9	87	333	29	288	4.23e-14	74.7
MS.gene022366.t1	MTR_2g069440	26.408	284	148	11	87	333	206	465	8.33e-14	74.3
MS.gene022366.t1	MTR_2g069440	26.855	283	148	11	87	333	29	288	8.38e-14	73.9
MS.gene022366.t1	MTR_1g052535	24.845	322	220	8	81	394	73	380	1.42e-13	73.2
MS.gene022366.t1	MTR_1g052535	24.845	322	220	8	81	394	73	380	2.72e-13	72.8
MS.gene022366.t1	MTR_4g119340	34.518	197	107	8	85	270	10	195	1.48e-12	70.9

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene022366.t1	AT4G20360	84.914	464	60	4	30	487	17	476	0.0	766
MS.gene022366.t1	AT4G20360	84.914	464	60	4	30	487	17	476	0.0	766
MS.gene022366.t1	AT4G02930	67.241	406	117	4	84	486	61	453	0.0	514
MS.gene022366.t1	AT5G60390	30.134	448	244	15	86	485	3	429	2.64e-44	162
MS.gene022366.t1	AT5G60390	30.134	448	244	15	86	485	3	429	2.64e-44	162
MS.gene022366.t1	AT5G60390	30.134	448	244	15	86	485	3	429	2.64e-44	162
MS.gene022366.t1	AT1G07920	30.134	448	244	15	86	485	3	429	2.64e-44	162
MS.gene022366.t1	AT1G07940	30.134	448	244	15	86	485	3	429	2.64e-44	162
MS.gene022366.t1	AT1G07940	30.134	448	244	15	86	485	3	429	2.64e-44	162
MS.gene022366.t1	AT1G07940	30.134	448	244	15	86	485	3	429	2.64e-44	162
MS.gene022366.t1	AT1G07940	30.134	448	244	15	86	485	3	429	2.64e-44	162
MS.gene022366.t1	AT1G07930	30.134	448	244	15	86	485	3	429	2.64e-44	162
MS.gene022366.t1	AT5G10630	29.452	438	258	14	90	487	315	741	3.13e-38	149
MS.gene022366.t1	AT5G10630	29.478	441	254	15	90	487	239	665	3.25e-38	148
MS.gene022366.t1	AT5G10630	29.478	441	254	15	90	487	240	666	3.27e-38	148
MS.gene022366.t1	AT5G10630	29.478	441	254	15	90	487	240	666	3.27e-38	148
MS.gene022366.t1	AT5G10630	29.452	438	258	14	90	487	310	736	3.88e-38	148
MS.gene022366.t1	AT1G18070	25.577	477	287	14	61	486	67	526	1.15e-31	128
MS.gene022366.t1	AT1G18070	25.577	477	287	14	61	486	67	526	1.15e-31	128
MS.gene022366.t1	AT1G07930	32.075	265	138	9	86	320	3	255	1.31e-30	122
MS.gene022366.t1	AT1G18070	25.205	488	286	15	61	486	67	537	5.56e-29	120
MS.gene022366.t1	AT2G31060	32.157	255	155	8	92	341	61	302	3.98e-21	97.4
MS.gene022366.t1	AT2G31060	32.046	259	154	9	92	341	61	306	2.82e-20	94.7
MS.gene022366.t1	AT5G13650	24.548	387	255	10	1	375	1	362	2.01e-16	82.8
MS.gene022366.t1	AT2G18720	27.143	280	149	11	87	333	35	292	4.77e-16	80.9
MS.gene022366.t1	AT2G18720	27.143	280	149	11	87	333	35	292	4.77e-16	80.9
MS.gene022366.t1	AT5G13650	24.041	391	251	10	1	375	1	361	1.53e-15	79.7
MS.gene022366.t1	AT1G04170	27.046	281	150	10	87	333	30	289	1.60e-15	79.3
MS.gene022366.t1	AT1G04170	27.046	281	150	10	87	333	30	289	1.60e-15	79.3
MS.gene022366.t1	AT4G18330	27.076	277	147	10	91	333	40	295	3.22e-15	78.2
MS.gene022366.t1	AT4G18330	29.843	191	105	7	91	255	40	227	9.35e-14	72.0
MS.gene022366.t1	AT5G39900	25.633	316	192	10	30	333	7	291	1.07e-12	70.9
MS.gene022366.t1	AT2G18720	27.368	190	108	5	153	333	33	201	1.14e-12	69.7

Find 156 sgRNAs with CRISPR-Local

Find 191 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
GGAGATGTTTCAGAAGATTT+TGG	0.174143	3.1:+63041920	MS.gene022366:CDS
GATGACATTCCGATTGTTTC+TGG	0.182010	3.1:+63041609	MS.gene022366:CDS
CACCTTTCTTTGCTGGTTAT+AGG	0.252585	3.1:+63042105	MS.gene022366:CDS
TGTTTCTGGTTCAGCGCTAT+TGG	0.258255	3.1:+63041623	MS.gene022366:CDS
GGATAAGATTTATAAACTTA+TGG	0.261517	3.1:+63041701	MS.gene022366:CDS
ATCCTCAATAGCTAGCAAAA+AGG	0.263547	3.1:-63041772	None:intergenic
AGGTGTGGGTGTGTGGTGGG+TGG	0.272409	3.1:-63040637	None:intergenic
GAGGCGAGTTAGGGTTAGTT+TGG	0.273503	3.1:-63040705	None:intergenic
AGGAGGAAGAGAGGCGAGTT+AGG	0.275765	3.1:-63040715	None:intergenic
GTGTGGGTGTGTGGTGGGTG+GGG	0.292383	3.1:-63040635	None:intergenic
GGTGTGGGTGTGTGGTGGGT+GGG	0.298078	3.1:-63040636	None:intergenic
GGAGCGAGTGGTGGTTGGTT+TGG	0.311291	3.1:-63040777	None:intergenic
AGGGGTGAGGTGGAGGATTG+TGG	0.312038	3.1:-63040750	None:intergenic
CGCTGCTCTCACTATGGCTT+TGG	0.317551	3.1:+63040904	MS.gene022366:CDS
ATTGGTGGAAGGGGAGGAAA+TGG	0.323140	3.1:-63040675	None:intergenic
TGGAGGAAGGGGTGAGGTGG+AGG	0.325736	3.1:-63040757	None:intergenic
AGCGAGAAGGATGTGTTCTT+TGG	0.340715	3.1:-63041164	None:intergenic
ACTTGTGGAGCTTGAAGTTC+GGG	0.340961	3.1:+63041557	MS.gene022366:CDS
GAGTGAGGAGTGATAGTTCC+AGG	0.341582	3.1:-63042029	None:intergenic
GTTTGGAGGAAGGGGTGAGG+TGG	0.344028	3.1:-63040760	None:intergenic
AGACATTCACCTTTCTTTGC+TGG	0.352845	3.1:+63042098	MS.gene022366:CDS
CTCTCGTCTTACGAATTTCC+TGG	0.356869	3.1:+63041585	MS.gene022366:CDS
TGAAGGTGTGGGTGTGTGGT+GGG	0.357080	3.1:-63040640	None:intergenic
TTGGTTTGGAGGAAGGGGTG+AGG	0.361287	3.1:-63040763	None:intergenic
GGGCGGCACGGACGGTGAAA+GGG	0.362945	3.1:-63040802	None:intergenic
ACCGTTGGAGCTGGTGTTAT+CGG	0.367859	3.1:+63042305	MS.gene022366:CDS
GGAGGATTGTGGAGGGGTTG+AGG	0.370592	3.1:-63040739	None:intergenic
AACTTGTGGAGCTTGAAGTT+CGG	0.370653	3.1:+63041556	MS.gene022366:CDS
TTATCACCACGTTTAATAGT+AGG	0.374526	3.1:-63041669	None:intergenic
GGGAGATCTGTTTGTCTTTG+AGG	0.374733	3.1:-63041750	None:intergenic
GGTTAGTTTGGGACGGAGAT+TGG	0.379136	3.1:-63040693	None:intergenic
GTGGTGGTTGGTTTGGAGGA+AGG	0.379395	3.1:-63040770	None:intergenic
TCTGTTTGTCTTTGAGGGAT+TGG	0.380993	3.1:-63041744	None:intergenic
TGGTGGTTGGTTTGGAGGAA+GGG	0.381462	3.1:-63040769	None:intergenic
GGAGATCTGTTTGTCTTTGA+GGG	0.382317	3.1:-63041749	None:intergenic
AGGCGAGTTAGGGTTAGTTT+GGG	0.389250	3.1:-63040704	None:intergenic
TATTCAAAGAGGGATGGTTT+TGG	0.389492	3.1:+63042001	MS.gene022366:CDS
TGTTCTTTGGTTTGAGGCAT+AGG	0.393640	3.1:-63041151	None:intergenic
CGGGCGGCACGGACGGTGAA+AGG	0.394872	3.1:-63040803	None:intergenic
ATACCACGAGCACGTTCTTC+AGG	0.396105	3.1:-63040977	None:intergenic
GTGAAGGTGTGGGTGTGTGG+TGG	0.407058	3.1:-63040641	None:intergenic
ACGGAGATTGGTGGAAGGGG+AGG	0.407272	3.1:-63040681	None:intergenic
GGAGGAAATGGAAGCGGAAG+TGG	0.408836	3.1:-63040663	None:intergenic
GGAGGAAGAGAGGCGAGTTA+GGG	0.409378	3.1:-63040714	None:intergenic
GCGAGTGGTGGTTGGTTTGG+AGG	0.412925	3.1:-63040774	None:intergenic
GGTGGTTGGTTTGGAGGAAG+GGG	0.418455	3.1:-63040768	None:intergenic
GTGGTGAAGGTGTGGGTGTG+TGG	0.422895	3.1:-63040644	None:intergenic
GGGACGGAGATTGGTGGAAG+GGG	0.423977	3.1:-63040684	None:intergenic
TTTAACCGCTGCTCTCACTA+TGG	0.435485	3.1:+63040898	MS.gene022366:CDS
GGGTGATGAGAGTTTGAGTT+TGG	0.436789	3.1:-63040615	None:intergenic
GGCGGCACGGACGGTGAAAG+GGG	0.436818	3.1:-63040801	None:intergenic
GTGAGGTGGAGGATTGTGGA+GGG	0.438591	3.1:-63040746	None:intergenic
ATTGGCGTTGGAAGCATTAG+TGG	0.440962	3.1:+63041641	MS.gene022366:CDS
TGAGGTGGAGGATTGTGGAG+GGG	0.441869	3.1:-63040745	None:intergenic
TATGTCAAGAATATGATTAC+TGG	0.446601	3.1:+63041079	MS.gene022366:CDS
GATTGTGGAGGGGTTGAGGA+AGG	0.459794	3.1:-63040735	None:intergenic
ATGAGGTTTGCTATTAGAGA+AGG	0.465146	3.1:+63042275	MS.gene022366:CDS
GGCGCTATTCTCGTTGTATC+CGG	0.467853	3.1:+63041118	MS.gene022366:CDS
GGTTGAGGAAGGAGGAAGAG+AGG	0.468935	3.1:-63040724	None:intergenic
GGTGAGGTGGAGGATTGTGG+AGG	0.468994	3.1:-63040747	None:intergenic
GGTGATGTAGTTGACCTTGT+TGG	0.469409	3.1:+63041861	MS.gene022366:CDS
GGCACGGACGGTGAAAGGGG+AGG	0.483277	3.1:-63040798	None:intergenic
CAGAAGATTGATATTCAAAG+AGG	0.486716	3.1:+63041990	MS.gene022366:CDS
AGGTTTGCTATTAGAGAAGG+AGG	0.486983	3.1:+63042278	MS.gene022366:CDS
ATGAGAACCACAGATGTTAC+CGG	0.488905	3.1:+63042140	MS.gene022366:CDS
TAGTTGACCTTGTTGGTTTG+AGG	0.490161	3.1:+63041868	MS.gene022366:CDS
TGTGGAGGGGTTGAGGAAGG+AGG	0.491699	3.1:-63040732	None:intergenic
TATCACCACGTTTAATAGTA+GGG	0.492921	3.1:-63041668	None:intergenic
TGCCACCGGAAGAATTGAAA+GGG	0.496315	3.1:+63041824	MS.gene022366:CDS
AGTTGACCTTGTTGGTTTGA+GGG	0.496349	3.1:+63041869	MS.gene022366:CDS
CTCGTTGTATCCGGTGCCGA+TGG	0.498113	3.1:+63041127	MS.gene022366:CDS
GGGGAGGAGCGAGTGGTGGT+TGG	0.499727	3.1:-63040782	None:intergenic
CACCATCTTAACACGATCAC+CGG	0.500532	3.1:-63042216	None:intergenic
CGAATTTGCCACGGGCGGCA+CGG	0.503769	3.1:-63040814	None:intergenic
TTTGCCACGGGCGGCACGGA+CGG	0.506319	3.1:-63040810	None:intergenic
TTTGCGCTCGAATTTGCCAC+GGG	0.508126	3.1:-63040822	None:intergenic
GATTACTGGTGCTGCTCAGA+TGG	0.508789	3.1:+63041093	MS.gene022366:CDS
AGGATGTGTTCTTTGGTTTG+AGG	0.511066	3.1:-63041157	None:intergenic
ACACATCCTTCTCGCTAAGC+AGG	0.511666	3.1:+63041171	MS.gene022366:CDS
TGGTTCAGCGCTATTGGCGT+TGG	0.516474	3.1:+63041629	MS.gene022366:CDS
GCCATGGTCGACGTGGCCGA+TGG	0.516822	3.1:-63040867	None:intergenic
TAGTTTGGGACGGAGATTGG+TGG	0.518165	3.1:-63040690	None:intergenic
ATTCCTTGTTCAATAGCCAC+AGG	0.523741	3.1:-63042254	None:intergenic
TGAGGAGCTTCTTGAACTTG+TGG	0.528672	3.1:+63041542	MS.gene022366:CDS
AGAGAAGGAGGGAAGACCGT+TGG	0.533076	3.1:+63042290	MS.gene022366:CDS
ACAATCGGAATGTCATCTCC+AGG	0.533175	3.1:-63041603	None:intergenic
CTTGACATAATCAGCGTGAC+CGG	0.533310	3.1:-63041065	None:intergenic
TTCAAGAACACAACCACACT+CGG	0.534270	3.1:-63041498	None:intergenic
TTCACCGTCCGTGCCGCCCG+TGG	0.534451	3.1:+63040806	MS.gene022366:CDS
TGGGACGGAGATTGGTGGAA+GGG	0.535402	3.1:-63040685	None:intergenic
TTGACATAATCAGCGTGACC+GGG	0.536185	3.1:-63041064	None:intergenic
GGAGGGAAGACCGTTGGAGC+TGG	0.536449	3.1:+63042296	MS.gene022366:CDS
GGCCTATAACCAGCAAAGAA+AGG	0.537786	3.1:-63042107	None:intergenic
ACTATGGCTTTGGCTTCTCT+AGG	0.537993	3.1:+63040914	MS.gene022366:CDS
GGTGAAAGGGGAGGAGCGAG+TGG	0.540841	3.1:-63040789	None:intergenic
AAGCGGAAGTGGTGAAGGTG+TGG	0.545502	3.1:-63040652	None:intergenic
TATGCTCATGTTGATTGCCC+CGG	0.546751	3.1:+63041046	MS.gene022366:CDS
AATGGAAGCGGAAGTGGTGA+AGG	0.549887	3.1:-63040657	None:intergenic
CAAGGATGAGGAGTCGAAGA+TGG	0.551958	3.1:+63042187	MS.gene022366:CDS
AGTGAGAGCAGCGGTTAAAG+TGG	0.552672	3.1:-63040894	None:intergenic
GGTTTGAGGCATAGGACCAT+CGG	0.555353	3.1:-63041143	None:intergenic
TAATTACCTGCTTAGCGAGA+AGG	0.556327	3.1:-63041177	None:intergenic
ACGAGGAGCACTACAGTGAC+CGG	0.557284	3.1:+63041894	MS.gene022366:CDS
AGCGGAAGTGGTGAAGGTGT+GGG	0.561639	3.1:-63040651	None:intergenic
GGTTTGCTATTAGAGAAGGA+GGG	0.562103	3.1:+63042279	MS.gene022366:CDS
AGCGCTGAACCAGAAACAAT+CGG	0.562250	3.1:-63041618	None:intergenic
GGAAGGGGAGGAAATGGAAG+CGG	0.564051	3.1:-63040669	None:intergenic
CATCTCAACATCGGAACCAT+CGG	0.564830	3.1:+63040851	MS.gene022366:CDS
TTGGGACGGAGATTGGTGGA+AGG	0.565908	3.1:-63040686	None:intergenic
AGGCATAGGACCATCGGCAC+CGG	0.568713	3.1:-63041137	None:intergenic
GAGTTAGGGTTAGTTTGGGA+CGG	0.569223	3.1:-63040700	None:intergenic
GATTGATATTCAAAGAGGGA+TGG	0.573204	3.1:+63041995	MS.gene022366:CDS
TCCGATAACACCAGCTCCAA+CGG	0.573833	3.1:-63042306	None:intergenic
GAAAGGGGAGGAGCGAGTGG+TGG	0.575537	3.1:-63040786	None:intergenic
TTGCCACCGGAAGAATTGAA+AGG	0.577024	3.1:+63041823	MS.gene022366:CDS
GTCCCTGTGGCTATTGAACA+AGG	0.580048	3.1:+63042251	MS.gene022366:CDS
TGCTATGGCTGGTGATAACG+TGG	0.580824	3.1:+63041947	MS.gene022366:CDS
TTAAACGTGGTGATAACGAG+TGG	0.581448	3.1:+63041676	MS.gene022366:CDS
TGGCTATTGAACAAGGAATG+AGG	0.586309	3.1:+63042258	MS.gene022366:CDS
TATCATGAATGACAAGGATG+AGG	0.592077	3.1:+63042175	MS.gene022366:CDS
GCCCGGTGATCGTGTTAAGA+TGG	0.593994	3.1:+63042214	MS.gene022366:CDS
GAAGCTCCTCATCATCCACT+TGG	0.605442	3.1:-63041530	None:intergenic
ACCATCTTAACACGATCACC+GGG	0.606257	3.1:-63042215	None:intergenic
ACCATCGGCCACGTCGACCA+TGG	0.607333	3.1:+63040866	MS.gene022366:CDS
AGAAGATTGATATTCAAAGA+GGG	0.608439	3.1:+63041991	MS.gene022366:CDS
TGAAGAAATTGAAATTGCCA+TGG	0.612079	3.1:-63040582	None:intergenic
TTAGGTTGGTGTTCCGAGTG+TGG	0.613093	3.1:+63041485	MS.gene022366:intron
GCGCTCGAATTTGCCACGGG+CGG	0.613444	3.1:-63040819	None:intergenic
GGTTGTGTTCTTGAACAAGC+AGG	0.616431	3.1:+63041506	MS.gene022366:CDS
GCAGGACCAAGTGGATGATG+AGG	0.618136	3.1:+63041524	MS.gene022366:CDS
ATGGTTCCGATGTTGAGATG+AGG	0.622236	3.1:-63040848	None:intergenic
TGATCGTGTTAAGATGGTGG+TGG	0.628826	3.1:+63042220	MS.gene022366:CDS
ACTGGTGCTGCTCAGATGGA+CGG	0.628856	3.1:+63041097	MS.gene022366:CDS
CAAAGCCATAGTGAGAGCAG+CGG	0.630312	3.1:-63040903	None:intergenic
GGTCGTGGAACAGTTGCCAC+CGG	0.632950	3.1:+63041810	MS.gene022366:CDS
GCTCCTGAAGAACGTGCTCG+TGG	0.633471	3.1:+63040974	MS.gene022366:CDS
TTGTTGGTTTGAGGGAAACG+AGG	0.634090	3.1:+63041877	MS.gene022366:CDS
GAGTCGAAGATGGTGATGCC+CGG	0.639572	3.1:+63042197	MS.gene022366:CDS
CGGTGATCGTGTTAAGATGG+TGG	0.643732	3.1:+63042217	MS.gene022366:CDS
TCGTTTCCCTCAAACCAACA+AGG	0.646895	3.1:-63041875	None:intergenic
ACGTGGTGATAACGAGTGGG+TGG	0.647355	3.1:+63041680	MS.gene022366:CDS
TGACATAATCAGCGTGACCG+GGG	0.648541	3.1:-63041063	None:intergenic
GGTGGAACTCATTGTCCCTG+TGG	0.649883	3.1:+63042238	MS.gene022366:CDS
TTCCTTGTTCAATAGCCACA+GGG	0.659471	3.1:-63042253	None:intergenic
ATACATTCAAACTTAGAGTG+AGG	0.660448	3.1:-63042044	None:intergenic
CTTGAACAAGCAGGACCAAG+TGG	0.660677	3.1:+63041515	MS.gene022366:CDS
CTTCTGAAACATCTCCACTC+CGG	0.669147	3.1:-63041913	None:intergenic
TAAACGTGGTGATAACGAGT+GGG	0.671761	3.1:+63041677	MS.gene022366:CDS
CCACCGGAAGAATTGAAAGG+GGG	0.679857	3.1:+63041826	MS.gene022366:CDS
GAGCACTACAGTGACCGGAG+TGG	0.680558	3.1:+63041899	MS.gene022366:CDS
AACGTGGGTTTGTTGCTGAG+AGG	0.684650	3.1:+63041963	MS.gene022366:CDS
GCCACCGGAAGAATTGAAAG+GGG	0.695004	3.1:+63041825	MS.gene022366:CDS
GTGGTTCTCATGTAAAACTG+AGG	0.696417	3.1:-63042128	None:intergenic
TACATCTATCATGAATGACA+AGG	0.699849	3.1:+63042169	MS.gene022366:CDS
GCAAACCCTACTATTAAACG+TGG	0.729265	3.1:+63041663	MS.gene022366:CDS
GCTATGGCTGGTGATAACGT+GGG	0.737390	3.1:+63041948	MS.gene022366:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!!	TAATACTAATAATTTTAGTT+AGG	+	chr3.1:63041248-63041267	MS.gene022366:intron	10.0%
!!	GGATAAGATTTATAAACTTA+TGG	+	chr3.1:63041701-63041720	MS.gene022366:CDS	20.0%
!!!	AATTTCATCGTATTTTTTAG+GGG	-	chr3.1:63040948-63040967	None:intergenic	20.0%
!!!	ATGTTTGTTTGTTTTTTGAT+TGG	+	chr3.1:63041454-63041473	MS.gene022366:intron	20.0%
!!!	CAATTTCATCGTATTTTTTA+GGG	-	chr3.1:63040949-63040968	None:intergenic	20.0%
!!!	TCAATTTCATCGTATTTTTT+AGG	-	chr3.1:63040950-63040969	None:intergenic	20.0%
!!!	TCTAGTCTGTATATAAATTT+TGG	+	chr3.1:63041383-63041402	MS.gene022366:intron	20.0%
!	AGAAGATTGATATTCAAAGA+GGG	+	chr3.1:63041991-63042010	MS.gene022366:CDS	25.0%
!	TATGTCAAGAATATGATTAC+TGG	+	chr3.1:63041079-63041098	MS.gene022366:CDS	25.0%
!!!	TATTGTGTATGTTTTGAAGA+AGG	+	chr3.1:63042064-63042083	MS.gene022366:CDS	25.0%
!!!	TTGTTTGTTTTTTGATTGGT+TGG	+	chr3.1:63041458-63041477	MS.gene022366:intron	25.0%
!!!	TTTTGAATCGTTGGTTTTAA+TGG	+	chr3.1:63041426-63041445	MS.gene022366:intron	25.0%
	ATACATTCAAACTTAGAGTG+AGG	-	chr3.1:63042047-63042066	None:intergenic	30.0%
	CAGAAGATTGATATTCAAAG+AGG	+	chr3.1:63041990-63042009	MS.gene022366:CDS	30.0%
	TACATCTATCATGAATGACA+AGG	+	chr3.1:63042169-63042188	MS.gene022366:CDS	30.0%
	TAGTGATGAATTAATGCAAC+AGG	+	chr3.1:63041318-63041337	MS.gene022366:intron	30.0%
	TATCACCACGTTTAATAGTA+GGG	-	chr3.1:63041671-63041690	None:intergenic	30.0%
	TTATCACCACGTTTAATAGT+AGG	-	chr3.1:63041672-63041691	None:intergenic	30.0%
!	ATGTATGGTTTTTGAATCGT+TGG	+	chr3.1:63041417-63041436	MS.gene022366:intron	30.0%
!	CATGATAGATGTAACTTTTC+CGG	-	chr3.1:63042162-63042181	None:intergenic	30.0%
!	TTGGTGATTATCTTCATGTA+TGG	+	chr3.1:63041402-63041421	MS.gene022366:intron	30.0%
!!	TTTTGATTGGTTGGTATCTT+AGG	+	chr3.1:63041467-63041486	MS.gene022366:intron	30.0%
!!!	TATGTTTTGAAGAAGGAAGA+AGG	+	chr3.1:63042071-63042090	MS.gene022366:CDS	30.0%
	AAAAAGCCTCATCTCAACAT+CGG	+	chr3.1:63040842-63040861	MS.gene022366:CDS	35.0%
	ATCCTCAATAGCTAGCAAAA+AGG	-	chr3.1:63041775-63041794	None:intergenic	35.0%
	GAATTAATGCAACAGGTGTA+TGG	+	chr3.1:63041325-63041344	MS.gene022366:intron	35.0%
	GATTGATATTCAAAGAGGGA+TGG	+	chr3.1:63041995-63042014	MS.gene022366:CDS	35.0%
	TATCATGAATGACAAGGATG+AGG	+	chr3.1:63042175-63042194	MS.gene022366:CDS	35.0%
	TCCTCAATAGCTAGCAAAAA+GGG	-	chr3.1:63041774-63041793	None:intergenic	35.0%
!	GGAGATGTTTCAGAAGATTT+TGG	+	chr3.1:63041920-63041939	MS.gene022366:CDS	35.0%
!	TATTCAAAGAGGGATGGTTT+TGG	+	chr3.1:63042001-63042020	MS.gene022366:CDS	35.0%
!!	ATGAGGTTTGCTATTAGAGA+AGG	+	chr3.1:63042275-63042294	MS.gene022366:CDS	35.0%
	AACTTGTGGAGCTTGAAGTT+CGG	+	chr3.1:63041556-63041575	MS.gene022366:CDS	40.0%
	AGACATTCACCTTTCTTTGC+TGG	+	chr3.1:63042098-63042117	MS.gene022366:CDS	40.0%
	ATGAGAACCACAGATGTTAC+CGG	+	chr3.1:63042140-63042159	MS.gene022366:CDS	40.0%
	ATTCCTTGTTCAATAGCCAC+AGG	-	chr3.1:63042257-63042276	None:intergenic	40.0%
	CCTCAATAGCTAGCAAAAAG+GGG	-	chr3.1:63041773-63041792	None:intergenic	40.0%
	GATGACATTCCGATTGTTTC+TGG	+	chr3.1:63041609-63041628	MS.gene022366:CDS	40.0%
	GCAAACCCTACTATTAAACG+TGG	+	chr3.1:63041663-63041682	MS.gene022366:CDS	40.0%
	GCAACAGGTGTATGGAATTT+AGG	+	chr3.1:63041333-63041352	MS.gene022366:intron	40.0%
	GGAGATCTGTTTGTCTTTGA+GGG	-	chr3.1:63041752-63041771	None:intergenic	40.0%
	GTGGTTCTCATGTAAAACTG+AGG	-	chr3.1:63042131-63042150	None:intergenic	40.0%
	TAATTACCTGCTTAGCGAGA+AGG	-	chr3.1:63041180-63041199	None:intergenic	40.0%
	TGGCTATTGAACAAGGAATG+AGG	+	chr3.1:63042258-63042277	MS.gene022366:CDS	40.0%
	TTCAAGAACACAACCACACT+CGG	-	chr3.1:63041501-63041520	None:intergenic	40.0%
	TTCCTTGTTCAATAGCCACA+GGG	-	chr3.1:63042256-63042275	None:intergenic	40.0%
!	AACTTTTCCGGTAACATCTG+TGG	-	chr3.1:63042150-63042169	None:intergenic	40.0%
!	CACCTTTCTTTGCTGGTTAT+AGG	+	chr3.1:63042105-63042124	MS.gene022366:CDS	40.0%
!	GAAGATTTTGGACGATGCTA+TGG	+	chr3.1:63041932-63041951	MS.gene022366:CDS	40.0%
!	GAGGATGTGTTTTCAATCAC+TGG	+	chr3.1:63041789-63041808	MS.gene022366:CDS	40.0%
!	GATTGGTTGGTATCTTAGGT+TGG	+	chr3.1:63041471-63041490	MS.gene022366:intron	40.0%
!	TAAACGTGGTGATAACGAGT+GGG	+	chr3.1:63041677-63041696	MS.gene022366:CDS	40.0%
!	TCTGTTTGTCTTTGAGGGAT+TGG	-	chr3.1:63041747-63041766	None:intergenic	40.0%
!	TTAAACGTGGTGATAACGAG+TGG	+	chr3.1:63041676-63041695	MS.gene022366:CDS	40.0%
!!	AATTGAAAGGGGGCTTGTTA+AGG	+	chr3.1:63041836-63041855	MS.gene022366:CDS	40.0%
!!	AGGATGTGTTCTTTGGTTTG+AGG	-	chr3.1:63041160-63041179	None:intergenic	40.0%
!!	AGGTTTGCTATTAGAGAAGG+AGG	+	chr3.1:63042278-63042297	MS.gene022366:CDS	40.0%
!!	AGTTGACCTTGTTGGTTTGA+GGG	+	chr3.1:63041869-63041888	MS.gene022366:CDS	40.0%
!!	GGTTTGCTATTAGAGAAGGA+GGG	+	chr3.1:63042279-63042298	MS.gene022366:CDS	40.0%
!!	TAGTTGACCTTGTTGGTTTG+AGG	+	chr3.1:63041868-63041887	MS.gene022366:CDS	40.0%
!!	TGTTCTTTGGTTTGAGGCAT+AGG	-	chr3.1:63041154-63041173	None:intergenic	40.0%
!!!	GTTTTGAAGAAGGAAGAAGG+TGG	+	chr3.1:63042074-63042093	MS.gene022366:CDS	40.0%
	AAGCCCCCTTTCAATTCTTC+CGG	-	chr3.1:63041832-63041851	None:intergenic	45.0%
	ACAATCGGAATGTCATCTCC+AGG	-	chr3.1:63041606-63041625	None:intergenic	45.0%
	ACCATCTTAACACGATCACC+GGG	-	chr3.1:63042218-63042237	None:intergenic	45.0%
	ACTTGTGGAGCTTGAAGTTC+GGG	+	chr3.1:63041557-63041576	MS.gene022366:CDS	45.0%
	AGCGAGAAGGATGTGTTCTT+TGG	-	chr3.1:63041167-63041186	None:intergenic	45.0%
	ATGGTTCCGATGTTGAGATG+AGG	-	chr3.1:63040851-63040870	None:intergenic	45.0%
	CACCATCTTAACACGATCAC+CGG	-	chr3.1:63042219-63042238	None:intergenic	45.0%
	CATCTCAACATCGGAACCAT+CGG	+	chr3.1:63040851-63040870	MS.gene022366:CDS	45.0%
	CTCTCGTCTTACGAATTTCC+TGG	+	chr3.1:63041585-63041604	MS.gene022366:CDS	45.0%
	CTTGACATAATCAGCGTGAC+CGG	-	chr3.1:63041068-63041087	None:intergenic	45.0%
	GGCCTATAACCAGCAAAGAA+AGG	-	chr3.1:63042110-63042129	None:intergenic	45.0%
	GGGAGATCTGTTTGTCTTTG+AGG	-	chr3.1:63041753-63041772	None:intergenic	45.0%
	GGTGATGTAGTTGACCTTGT+TGG	+	chr3.1:63041861-63041880	MS.gene022366:CDS	45.0%
	GGTTGTGTTCTTGAACAAGC+AGG	+	chr3.1:63041506-63041525	MS.gene022366:CDS	45.0%
	TCGTTTCCCTCAAACCAACA+AGG	-	chr3.1:63041878-63041897	None:intergenic	45.0%
	TGAGGAGCTTCTTGAACTTG+TGG	+	chr3.1:63041542-63041561	MS.gene022366:CDS	45.0%
	TGCCACCGGAAGAATTGAAA+GGG	+	chr3.1:63041824-63041843	MS.gene022366:CDS	45.0%
	TTGACATAATCAGCGTGACC+GGG	-	chr3.1:63041067-63041086	None:intergenic	45.0%
	TTGCCACCGGAAGAATTGAA+AGG	+	chr3.1:63041823-63041842	MS.gene022366:CDS	45.0%
	TTTAACCGCTGCTCTCACTA+TGG	+	chr3.1:63040898-63040917	MS.gene022366:CDS	45.0%
!	AGCGCTGAACCAGAAACAAT+CGG	-	chr3.1:63041621-63041640	None:intergenic	45.0%
!	ATTTTGGACGATGCTATGGC+TGG	+	chr3.1:63041936-63041955	MS.gene022366:CDS	45.0%
!	CCCCTTTTTGCTAGCTATTG+AGG	+	chr3.1:63041770-63041789	MS.gene022366:CDS	45.0%
!	GGGTGATGAGAGTTTGAGTT+TGG	-	chr3.1:63040618-63040637	None:intergenic	45.0%
!	GTGTTTTCAATCACTGGTCG+TGG	+	chr3.1:63041795-63041814	MS.gene022366:CDS	45.0%
!	TATGCTCATGTTGATTGCCC+CGG	+	chr3.1:63041046-63041065	MS.gene022366:CDS	45.0%
!	TGATCGTGTTAAGATGGTGG+TGG	+	chr3.1:63042220-63042239	MS.gene022366:CDS	45.0%
!	TTTTGCGCTCGAATTTGCCA+CGG	-	chr3.1:63040826-63040845	None:intergenic	45.0%
!!	ACTATGGCTTTGGCTTCTCT+AGG	+	chr3.1:63040914-63040933	MS.gene022366:CDS	45.0%
!!	AGGCGAGTTAGGGTTAGTTT+GGG	-	chr3.1:63040707-63040726	None:intergenic	45.0%
!!	ATTGGCGTTGGAAGCATTAG+TGG	+	chr3.1:63041641-63041660	MS.gene022366:CDS	45.0%
!!	CGGTTAAAGTGGTTTTGCCA+TGG	-	chr3.1:63040886-63040905	None:intergenic	45.0%
!!	CTTCTGAAACATCTCCACTC+CGG	-	chr3.1:63041916-63041935	None:intergenic	45.0%
!!	GAGTTAGGGTTAGTTTGGGA+CGG	-	chr3.1:63040703-63040722	None:intergenic	45.0%
!!	TGTTTCTGGTTCAGCGCTAT+TGG	+	chr3.1:63041623-63041642	MS.gene022366:CDS	45.0%
!!	TTGTTGGTTTGAGGGAAACG+AGG	+	chr3.1:63041877-63041896	MS.gene022366:CDS	45.0%
	AATGGAAGCGGAAGTGGTGA+AGG	-	chr3.1:63040660-63040679	None:intergenic	50.0%
	ACACATCCTTCTCGCTAAGC+AGG	+	chr3.1:63041171-63041190	MS.gene022366:CDS	50.0%
	AGTGAGAGCAGCGGTTAAAG+TGG	-	chr3.1:63040897-63040916	None:intergenic	50.0%
	ATACCACGAGCACGTTCTTC+AGG	-	chr3.1:63040980-63040999	None:intergenic	50.0%
	ATTGGTGGAAGGGGAGGAAA+TGG	-	chr3.1:63040678-63040697	None:intergenic	50.0%
	CAAAGCCATAGTGAGAGCAG+CGG	-	chr3.1:63040906-63040925	None:intergenic	50.0%
	CAAGGATGAGGAGTCGAAGA+TGG	+	chr3.1:63042187-63042206	MS.gene022366:CDS	50.0%
	CCACCGGAAGAATTGAAAGG+GGG	+	chr3.1:63041826-63041845	MS.gene022366:CDS	50.0%
	CGGTGATCGTGTTAAGATGG+TGG	+	chr3.1:63042217-63042236	MS.gene022366:CDS	50.0%
	CTTGAACAAGCAGGACCAAG+TGG	+	chr3.1:63041515-63041534	MS.gene022366:CDS	50.0%
	GAAGCTCCTCATCATCCACT+TGG	-	chr3.1:63041533-63041552	None:intergenic	50.0%
	GAGTGAGGAGTGATAGTTCC+AGG	-	chr3.1:63042032-63042051	None:intergenic	50.0%
	GCCACCGGAAGAATTGAAAG+GGG	+	chr3.1:63041825-63041844	MS.gene022366:CDS	50.0%
	GGCGCTATTCTCGTTGTATC+CGG	+	chr3.1:63041118-63041137	MS.gene022366:CDS	50.0%
	GTCCCTGTGGCTATTGAACA+AGG	+	chr3.1:63042251-63042270	MS.gene022366:CDS	50.0%
	TCCGATAACACCAGCTCCAA+CGG	-	chr3.1:63042309-63042328	None:intergenic	50.0%
	TGACATAATCAGCGTGACCG+GGG	-	chr3.1:63041066-63041085	None:intergenic	50.0%
	TTTGCGCTCGAATTTGCCAC+GGG	-	chr3.1:63040825-63040844	None:intergenic	50.0%
!	GCTATGGCTGGTGATAACGT+GGG	+	chr3.1:63041948-63041967	MS.gene022366:CDS	50.0%
!	GGTTTGAGGCATAGGACCAT+CGG	-	chr3.1:63041146-63041165	None:intergenic	50.0%
!	TAGTTTGGGACGGAGATTGG+TGG	-	chr3.1:63040693-63040712	None:intergenic	50.0%
!	TGCTATGGCTGGTGATAACG+TGG	+	chr3.1:63041947-63041966	MS.gene022366:CDS	50.0%
!!	AACGTGGGTTTGTTGCTGAG+AGG	+	chr3.1:63041963-63041982	MS.gene022366:CDS	50.0%
!!	ACCGTTGGAGCTGGTGTTAT+CGG	+	chr3.1:63042305-63042324	MS.gene022366:CDS	50.0%
!!	GAAAGGGGGCTTGTTAAGGT+TGG	+	chr3.1:63041840-63041859	MS.gene022366:CDS	50.0%
!!	GAGGCGAGTTAGGGTTAGTT+TGG	-	chr3.1:63040708-63040727	None:intergenic	50.0%
!!	GATTACTGGTGCTGCTCAGA+TGG	+	chr3.1:63041093-63041112	MS.gene022366:CDS	50.0%
!!	GGTTAGTTTGGGACGGAGAT+TGG	-	chr3.1:63040696-63040715	None:intergenic	50.0%
!!	TGGTGGTTGGTTTGGAGGAA+GGG	-	chr3.1:63040772-63040791	None:intergenic	50.0%
!!	TTAGGTTGGTGTTCCGAGTG+TGG	+	chr3.1:63041485-63041504	MS.gene022366:intron	50.0%
	AAGCGGAAGTGGTGAAGGTG+TGG	-	chr3.1:63040655-63040674	None:intergenic	55.0%
	AGAGAAGGAGGGAAGACCGT+TGG	+	chr3.1:63042290-63042309	MS.gene022366:CDS	55.0%
	AGGAGGAAGAGAGGCGAGTT+AGG	-	chr3.1:63040718-63040737	None:intergenic	55.0%
	CCCCCTTTCAATTCTTCCGG+TGG	-	chr3.1:63041829-63041848	None:intergenic	55.0%
	CGCTGCTCTCACTATGGCTT+TGG	+	chr3.1:63040904-63040923	MS.gene022366:CDS	55.0%
	GATTGTGGAGGGGTTGAGGA+AGG	-	chr3.1:63040738-63040757	None:intergenic	55.0%
	GCAGGACCAAGTGGATGATG+AGG	+	chr3.1:63041524-63041543	MS.gene022366:CDS	55.0%
	GCCCGGTGATCGTGTTAAGA+TGG	+	chr3.1:63042214-63042233	MS.gene022366:CDS	55.0%
	GGAAGGGGAGGAAATGGAAG+CGG	-	chr3.1:63040672-63040691	None:intergenic	55.0%
	GGAGGAAATGGAAGCGGAAG+TGG	-	chr3.1:63040666-63040685	None:intergenic	55.0%
	GGAGGAAGAGAGGCGAGTTA+GGG	-	chr3.1:63040717-63040736	None:intergenic	55.0%
	GGTGGAACTCATTGTCCCTG+TGG	+	chr3.1:63042238-63042257	MS.gene022366:CDS	55.0%
	GGTTGAGGAAGGAGGAAGAG+AGG	-	chr3.1:63040727-63040746	None:intergenic	55.0%
	GTGAGGTGGAGGATTGTGGA+GGG	-	chr3.1:63040749-63040768	None:intergenic	55.0%
	TGAGGTGGAGGATTGTGGAG+GGG	-	chr3.1:63040748-63040767	None:intergenic	55.0%
	TGGGACGGAGATTGGTGGAA+GGG	-	chr3.1:63040688-63040707	None:intergenic	55.0%
	TGGTTCAGCGCTATTGGCGT+TGG	+	chr3.1:63041629-63041648	MS.gene022366:CDS	55.0%
	TTGGGACGGAGATTGGTGGA+AGG	-	chr3.1:63040689-63040708	None:intergenic	55.0%
!	ACGTGGTGATAACGAGTGGG+TGG	+	chr3.1:63041680-63041699	MS.gene022366:CDS	55.0%
!	AGCGGAAGTGGTGAAGGTGT+GGG	-	chr3.1:63040654-63040673	None:intergenic	55.0%
!	GAGTCGAAGATGGTGATGCC+CGG	+	chr3.1:63042197-63042216	MS.gene022366:CDS	55.0%
!	TGAAGGTGTGGGTGTGTGGT+GGG	-	chr3.1:63040643-63040662	None:intergenic	55.0%
!!	ACGAGGAGCACTACAGTGAC+CGG	+	chr3.1:63041894-63041913	MS.gene022366:CDS	55.0%
!!	ACTGGTGCTGCTCAGATGGA+CGG	+	chr3.1:63041097-63041116	MS.gene022366:CDS	55.0%
!!	GGTGGTTGGTTTGGAGGAAG+GGG	-	chr3.1:63040771-63040790	None:intergenic	55.0%
!!	GTGGTGGTTGGTTTGGAGGA+AGG	-	chr3.1:63040773-63040792	None:intergenic	55.0%
!!	TGGTTTTGCCATGGTCGACG+TGG	-	chr3.1:63040877-63040896	None:intergenic	55.0%
!!	TTGGTTTGGAGGAAGGGGTG+AGG	-	chr3.1:63040766-63040785	None:intergenic	55.0%
!!!	GGGATGGTTTTGGCTAAGCC+TGG	+	chr3.1:63042011-63042030	MS.gene022366:CDS	55.0%
	ACGGAGATTGGTGGAAGGGG+AGG	-	chr3.1:63040684-63040703	None:intergenic	60.0%
	AGGCATAGGACCATCGGCAC+CGG	-	chr3.1:63041140-63041159	None:intergenic	60.0%
	AGGGGTGAGGTGGAGGATTG+TGG	-	chr3.1:63040753-63040772	None:intergenic	60.0%
	GAGCACTACAGTGACCGGAG+TGG	+	chr3.1:63041899-63041918	MS.gene022366:CDS	60.0%
	GCTCCTGAAGAACGTGCTCG+TGG	+	chr3.1:63040974-63040993	MS.gene022366:CDS	60.0%
	GGAGGATTGTGGAGGGGTTG+AGG	-	chr3.1:63040742-63040761	None:intergenic	60.0%
	GGGACGGAGATTGGTGGAAG+GGG	-	chr3.1:63040687-63040706	None:intergenic	60.0%
	GGTCGTGGAACAGTTGCCAC+CGG	+	chr3.1:63041810-63041829	MS.gene022366:CDS	60.0%
	GGTGAGGTGGAGGATTGTGG+AGG	-	chr3.1:63040750-63040769	None:intergenic	60.0%
	GTTTGGAGGAAGGGGTGAGG+TGG	-	chr3.1:63040763-63040782	None:intergenic	60.0%
	TGTGGAGGGGTTGAGGAAGG+AGG	-	chr3.1:63040735-63040754	None:intergenic	60.0%
!	CTCGTTGTATCCGGTGCCGA+TGG	+	chr3.1:63041127-63041146	MS.gene022366:CDS	60.0%
!	GGAGCGAGTGGTGGTTGGTT+TGG	-	chr3.1:63040780-63040799	None:intergenic	60.0%
!	GTGAAGGTGTGGGTGTGTGG+TGG	-	chr3.1:63040644-63040663	None:intergenic	60.0%
!	GTGGTGAAGGTGTGGGTGTG+TGG	-	chr3.1:63040647-63040666	None:intergenic	60.0%
!!	GCGAGTGGTGGTTGGTTTGG+AGG	-	chr3.1:63040777-63040796	None:intergenic	60.0%
	ACCATCGGCCACGTCGACCA+TGG	+	chr3.1:63040866-63040885	MS.gene022366:CDS	65.0%
	CGAATTTGCCACGGGCGGCA+CGG	-	chr3.1:63040817-63040836	None:intergenic	65.0%
	GAAAGGGGAGGAGCGAGTGG+TGG	-	chr3.1:63040789-63040808	None:intergenic	65.0%
	GCGCTCGAATTTGCCACGGG+CGG	-	chr3.1:63040822-63040841	None:intergenic	65.0%
	GGAGGGAAGACCGTTGGAGC+TGG	+	chr3.1:63042296-63042315	MS.gene022366:CDS	65.0%
	GGTGAAAGGGGAGGAGCGAG+TGG	-	chr3.1:63040792-63040811	None:intergenic	65.0%
	TGGAGGAAGGGGTGAGGTGG+AGG	-	chr3.1:63040760-63040779	None:intergenic	65.0%
!	AGGTGTGGGTGTGTGGTGGG+TGG	-	chr3.1:63040640-63040659	None:intergenic	65.0%
!!	GGTGTGGGTGTGTGGTGGGT+GGG	-	chr3.1:63040639-63040658	None:intergenic	65.0%
!!	GTGTGGGTGTGTGGTGGGTG+GGG	-	chr3.1:63040638-63040657	None:intergenic	65.0%
	GCCATGGTCGACGTGGCCGA+TGG	-	chr3.1:63040870-63040889	None:intergenic	70.0%
	GGCACGGACGGTGAAAGGGG+AGG	-	chr3.1:63040801-63040820	None:intergenic	70.0%
	GGCGGCACGGACGGTGAAAG+GGG	-	chr3.1:63040804-63040823	None:intergenic	70.0%
	GGGCGGCACGGACGGTGAAA+GGG	-	chr3.1:63040805-63040824	None:intergenic	70.0%
	GGGGAGGAGCGAGTGGTGGT+TGG	-	chr3.1:63040785-63040804	None:intergenic	70.0%
	TTTGCCACGGGCGGCACGGA+CGG	-	chr3.1:63040813-63040832	None:intergenic	70.0%
	CGGGCGGCACGGACGGTGAA+AGG	-	chr3.1:63040806-63040825	None:intergenic	75.0%
	TTCACCGTCCGTGCCGCCCG+TGG	+	chr3.1:63040806-63040825	MS.gene022366:CDS	75.0%

Chromosome	Type	Strat	End	Strand	Name
chr3.1	gene	63040584	63042343	63040584	ID=MS.gene022366
chr3.1	mRNA	63040584	63042343	63040584	ID=MS.gene022366.t1;Parent=MS.gene022366
chr3.1	exon	63040584	63041192	63040584	ID=MS.gene022366.t1.exon1;Parent=MS.gene022366.t1
chr3.1	CDS	63040584	63041192	63040584	ID=cds.MS.gene022366.t1;Parent=MS.gene022366.t1
chr3.1	exon	63041489	63042343	63041489	ID=MS.gene022366.t1.exon2;Parent=MS.gene022366.t1
chr3.1	CDS	63041489	63042343	63041489	ID=cds.MS.gene022366.t1;Parent=MS.gene022366.t1

Gene Sequence

>MS.gene022366

ATGGCAATTTCAATTTCTTCATCTTCTTCTACCAAACTCAAACTCTCATCACCCCACCCACCACACACCCACACCTTCACCACTTCCGCTTCCATTTCCTCCCCTTCCACCAATCTCCGTCCCAAACTAACCCTAACTCGCCTCTCTTCCTCCTTCCTCAACCCCTCCACAATCCTCCACCTCACCCCTTCCTCCAAACCAACCACCACTCGCTCCTCCCCTTTCACCGTCCGTGCCGCCCGTGGCAAATTCGAGCGCAAAAAGCCTCATCTCAACATCGGAACCATCGGCCACGTCGACCATGGCAAAACCACTTTAACCGCTGCTCTCACTATGGCTTTGGCTTCTCTAGGTAACAGTGCCCCTAAAAAATACGATGAAATTGACGCTGCTCCTGAAGAACGTGCTCGTGGTATTACAATTAATACTGCTACTGTTGAATATGAGACTGAAACTCGTCATTATGCTCATGTTGATTGCCCCGGTCACGCTGATTATGTCAAGAATATGATTACTGGTGCTGCTCAGATGGACGGCGCTATTCTCGTTGTATCCGGTGCCGATGGTCCTATGCCTCAAACCAAAGAACACATCCTTCTCGCTAAGCAGGTAATTATCTTATCTTAATCTAGTTATTAGATTAGTATTAGACTATTAGTAGTATTAATACTAATAATTTTAGTTAGGATTAGAATTAGTGAAGATTTAATGCAATGTTACACTATTGTTTAATGTAGTGATGAATTAATGCAACAGGTGTATGGAATTTAGGCGTGTGTAGAAGATTTATTGATAGATGTCTAGTCTGTATATAAATTTTGGTGATTATCTTCATGTATGGTTTTTGAATCGTTGGTTTTAATGGTTGAAATGTTTGTTTGTTTTTTGATTGGTTGGTATCTTAGGTTGGTGTTCCGAGTGTGGTTGTGTTCTTGAACAAGCAGGACCAAGTGGATGATGAGGAGCTTCTTGAACTTGTGGAGCTTGAAGTTCGGGAGCTACTCTCGTCTTACGAATTTCCTGGAGATGACATTCCGATTGTTTCTGGTTCAGCGCTATTGGCGTTGGAAGCATTAGTGGCAAACCCTACTATTAAACGTGGTGATAACGAGTGGGTGGATAAGATTTATAAACTTATGGATGAAGTTGACAACTATATACCAATCCCTCAAAGACAAACAGATCTCCCCTTTTTGCTAGCTATTGAGGATGTGTTTTCAATCACTGGTCGTGGAACAGTTGCCACCGGAAGAATTGAAAGGGGGCTTGTTAAGGTTGGTGATGTAGTTGACCTTGTTGGTTTGAGGGAAACGAGGAGCACTACAGTGACCGGAGTGGAGATGTTTCAGAAGATTTTGGACGATGCTATGGCTGGTGATAACGTGGGTTTGTTGCTGAGAGGTGTTCAGAAGATTGATATTCAAAGAGGGATGGTTTTGGCTAAGCCTGGAACTATCACTCCTCACTCTAAGTTTGAATGTATTGTGTATGTTTTGAAGAAGGAAGAAGGTGGAAGACATTCACCTTTCTTTGCTGGTTATAGGCCTCAGTTTTACATGAGAACCACAGATGTTACCGGAAAAGTTACATCTATCATGAATGACAAGGATGAGGAGTCGAAGATGGTGATGCCCGGTGATCGTGTTAAGATGGTGGTGGAACTCATTGTCCCTGTGGCTATTGAACAAGGAATGAGGTTTGCTATTAGAGAAGGAGGGAAGACCGTTGGAGCTGGTGTTATCGGAGCTATCATTGAGTGA

Protein sequence

>MS.gene022366.t1

MAISISSSSSTKLKLSSPHPPHTHTFTTSASISSPSTNLRPKLTLTRLSSSFLNPSTILHLTPSSKPTTTRSSPFTVRAARGKFERKKPHLNIGTIGHVDHGKTTLTAALTMALASLGNSAPKKYDEIDAAPEERARGITINTATVEYETETRHYAHVDCPGHADYVKNMITGAAQMDGAILVVSGADGPMPQTKEHILLAKQVGVPSVVVFLNKQDQVDDEELLELVELEVRELLSSYEFPGDDIPIVSGSALLALEALVANPTIKRGDNEWVDKIYKLMDEVDNYIPIPQRQTDLPFLLAIEDVFSITGRGTVATGRIERGLVKVGDVVDLVGLRETRSTTVTGVEMFQKILDDAMAGDNVGLLLRGVQKIDIQRGMVLAKPGTITPHSKFECIVYVLKKEEGGRHSPFFAGYRPQFYMRTTDVTGKVTSIMNDKDEESKMVMPGDRVKMVVELIVPVAIEQGMRFAIREGGKTVGAGVIGAIIE