AlfalfaGEDB | MS.gene028619

M. sativa cultivar XinJiangDaYe / MS.gene028619

KEGG: -
GO: GO:0003674 GO:0005488 GO:0005515
PFAM: PF12796.10 PF13962.9

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene028619.t1	PNY06919.1	94.6	425	21	1	1	425	1	423	5.70E-126	461.1

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene028619.t1	Q6AWW5	56.5	255	94	3	174	425	284	524	4.1e-64	246.9

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene028619.t1	A0A2K3NV51	94.6	425	21	1	1	425	1	423	4.1e-126	461.1

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR

PPI:

Gene1	Gene2	Type
MS.gene028619	MS.gene008653	PPI
MS.gene028619	MS.gene05138	PPI
MS.gene028619	MS.gene41484	PPI
MS.gene028619	MS.gene049555	PPI
MS.gene028619	MS.gene073427	PPI
MS.gene028619	MS.gene38420	PPI
MS.gene028619	MS.gene41487	PPI
MS.gene028619	MS.gene008452	PPI
MS.gene028619	MS.gene41481	PPI
MS.gene028619	MS.gene049551	PPI
MS.gene028619	MS.gene023218	PPI
MS.gene028619	MS.gene48445	PPI
MS.gene028619	MS.gene08407	PPI
MS.gene028619	MS.gene049563	PPI
MS.gene028619	MS.gene27240	PPI
MS.gene028619	MS.gene23793	PPI
MS.gene028619	MS.gene69986	PPI
MS.gene028619	MS.gene90199	PPI
MS.gene028619	MS.gene35208	PPI
MS.gene028619	MS.gene023219	PPI
MS.gene028619	MS.gene92274	PPI
MS.gene028619	MS.gene02178	PPI
MS.gene028619	MS.gene008465	PPI
MS.gene028619	MS.gene41475	PPI
MS.gene028619	MS.gene23794	PPI
MS.gene028619	MS.gene032323	PPI
MS.gene028619	MS.gene007855	PPI
MS.gene028619	MS.gene44512	PPI
MS.gene028619	MS.gene03927	PPI
MS.gene028619	MS.gene01192	PPI
MS.gene028619	MS.gene053559	PPI
MS.gene028619	MS.gene066483	PPI
MS.gene028619	MS.gene015265	PPI
MS.gene028619	MS.gene008451	PPI
MS.gene028619	MS.gene008654	PPI
MS.gene028619	MS.gene049562	PPI
MS.gene028619	MS.gene35210	PPI
MS.gene028619	MS.gene03929	PPI
MS.gene028619	MS.gene99572	PPI
MS.gene028619	MS.gene049576	PPI
MS.gene028619	MS.gene25041	PPI
MS.gene028619	MS.gene017540	PPI
MS.gene028619	MS.gene41482	PPI
MS.gene028619	MS.gene028458	PPI
MS.gene028619	MS.gene05756	PPI
MS.gene028619	MS.gene41488	PPI
MS.gene028619	MS.gene028243	PPI
MS.gene028619	MS.gene013038	PPI
MS.gene028619	MS.gene43534	PPI
MS.gene028619	MS.gene09607	PPI
MS.gene028619	MS.gene008470	PPI
MS.gene028619	MS.gene049560	PPI
MS.gene028619	MS.gene048532	PPI
MS.gene028619	MS.gene05753	PPI
MS.gene028619	MS.gene008474	PPI
MS.gene028619	MS.gene41476	PPI
MS.gene028619	MS.gene76441	PPI
MS.gene028619	MS.gene03932	PPI
MS.gene028619	MS.gene27241	PPI
MS.gene028619	MS.gene38427	PPI
MS.gene028619	MS.gene09550	PPI
MS.gene028619	MS.gene20251	PPI
MS.gene028619	MS.gene20305	PPI
MS.gene028619	MS.gene38996	PPI
MS.gene028619	MS.gene89606	PPI
MS.gene028619	MS.gene32240	PPI
MS.gene028619	MS.gene38422	PPI
MS.gene028619	MS.gene05139	PPI
MS.gene028619	MS.gene048534	PPI
MS.gene028619	MS.gene071898	PPI
MS.gene028619	MS.gene049552	PPI
MS.gene028619	MS.gene38433	PPI
MS.gene028619	MS.gene041920	PPI
MS.gene028619	MS.gene38428	PPI
MS.gene028619	MS.gene011756	PPI
MS.gene028619	MS.gene38426	PPI
MS.gene028619	MS.gene08405	PPI
MS.gene028619	MS.gene38425	PPI
MS.gene028619	MS.gene017541	PPI
MS.gene028619	MS.gene020402	PPI
MS.gene028619	MS.gene068045	PPI
MS.gene028619	MS.gene90448	PPI
MS.gene028619	MS.gene028244	PPI
MS.gene028619	MS.gene008655	PPI
MS.gene028619	MS.gene23830	PPI
MS.gene028619	MS.gene015211	PPI
MS.gene028619	MS.gene051024	PPI
MS.gene028619	MS.gene008464	PPI
MS.gene028619	MS.gene78702	PPI
MS.gene028619	MS.gene05752	PPI
MS.gene028619	MS.gene81040	PPI

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene028619.t1	MTR_2g096190	97.412	425	10	1	1	425	121	544	0.0	840
MS.gene028619.t1	MTR_2g096190	31.788	151	102	1	17	166	68	218	1.23e-15	79.3
MS.gene028619.t1	MTR_4g062100	78.638	426	86	3	1	425	125	546	0.0	647
MS.gene028619.t1	MTR_4g062100	32.143	168	100	6	15	172	31	194	9.64e-11	63.9
MS.gene028619.t1	MTR_7g100390	65.566	424	138	4	2	425	105	520	0.0	528
MS.gene028619.t1	MTR_7g117620	59.479	422	155	4	5	425	140	546	3.42e-169	486
MS.gene028619.t1	MTR_7g117620	33.540	161	95	7	19	170	47	204	2.75e-11	65.9
MS.gene028619.t1	MTR_1g115575	48.499	433	190	5	1	425	186	593	1.16e-128	384
MS.gene028619.t1	MTR_1g032100	46.729	428	210	7	5	425	115	531	6.47e-125	372
MS.gene028619.t1	MTR_8g105260	45.455	429	214	8	5	425	122	538	2.89e-121	363
MS.gene028619.t1	MTR_7g100430	46.387	429	202	6	1	425	178	582	1.32e-106	327
MS.gene028619.t1	MTR_2g101310	25.358	489	276	10	5	423	162	631	1.43e-35	139
MS.gene028619.t1	MTR_2g101310	27.485	171	124	0	1	171	90	260	8.04e-12	67.4
MS.gene028619.t1	MTR_1g069110	27.072	362	225	10	9	347	44	389	3.69e-27	113
MS.gene028619.t1	MTR_2g101320	24.408	422	236	10	5	355	162	571	1.61e-26	112
MS.gene028619.t1	MTR_2g101320	25.581	172	128	0	1	172	90	261	7.77e-12	67.8
MS.gene028619.t1	MTR_2g101310	25.538	325	168	7	5	261	162	480	2.16e-18	87.4
MS.gene028619.t1	MTR_2g101310	27.485	171	124	0	1	171	90	260	4.19e-11	65.1
MS.gene028619.t1	MTR_5g085830	25.199	377	226	11	12	357	306	657	4.97e-18	87.0
MS.gene028619.t1	MTR_5g085810	24.877	406	215	12	1	357	321	685	2.14e-17	85.1
MS.gene028619.t1	MTR_5g085480	25.916	382	234	10	12	357	113	481	1.94e-16	81.6
MS.gene028619.t1	MTR_5g085480	25.916	382	234	10	12	357	273	641	2.85e-16	81.6
MS.gene028619.t1	MTR_8g066670	22.558	430	239	12	1	390	1127	1502	5.41e-16	80.9
MS.gene028619.t1	MTR_5g043640	31.694	183	118	5	12	192	51	228	6.11e-16	77.4
MS.gene028619.t1	MTR_5g085500	32.530	166	105	3	11	169	1	166	1.24e-15	79.0
MS.gene028619.t1	MTR_8g080080	36.709	158	83	5	18	158	188	345	1.03e-14	76.6
MS.gene028619.t1	MTR_8g080080	26.667	165	105	3	12	160	218	382	2.63e-11	65.9
MS.gene028619.t1	MTR_3g105650	22.744	277	175	5	1	261	57	310	3.14e-14	74.3
MS.gene028619.t1	MTR_7g099570	31.548	168	105	4	13	171	39	205	1.75e-13	72.8
MS.gene028619.t1	MTR_7g099570	31.333	150	93	4	1	141	61	209	3.05e-12	68.9
MS.gene028619.t1	MTR_8g073260	24.542	273	169	6	1	261	63	310	2.27e-13	72.0
MS.gene028619.t1	MTR_2g033050	31.288	163	105	3	1	156	218	380	3.24e-13	71.2
MS.gene028619.t1	MTR_3g085830	41.121	107	62	1	18	124	185	290	3.66e-13	71.6
MS.gene028619.t1	MTR_3g085830	28.750	160	98	4	12	156	213	371	7.42e-12	67.8
MS.gene028619.t1	MTR_8g467780	23.103	290	166	6	1	263	63	322	1.03e-12	70.1
MS.gene028619.t1	MTR_8g467780	28.378	148	105	1	17	163	45	192	1.67e-11	66.2
MS.gene028619.t1	MTR_5g026550	37.903	124	68	4	8	124	22	143	1.31e-12	66.2
MS.gene028619.t1	MTR_4g113530	28.866	194	128	5	12	200	548	736	2.70e-12	69.3
MS.gene028619.t1	MTR_8g040930	23.214	336	205	10	1	309	11	320	5.72e-12	67.4
MS.gene028619.t1	MTR_8g041240	23.214	336	205	10	1	309	11	320	5.72e-12	67.4
MS.gene028619.t1	MTR_1g112830	27.684	177	119	3	2	171	115	289	1.71e-11	66.6
MS.gene028619.t1	MTR_6g022830	31.461	178	102	5	12	172	221	395	3.22e-11	65.9
MS.gene028619.t1	MTR_6g023070	31.461	178	102	5	12	172	158	332	3.50e-11	65.5
MS.gene028619.t1	MTR_8g062410	24.870	193	130	2	1	181	62	251	8.26e-11	63.9

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene028619.t1	AT1G07710	76.415	424	98	1	2	425	122	543	0.0	617
MS.gene028619.t1	AT1G07710	76.415	424	98	1	2	425	122	543	0.0	617
MS.gene028619.t1	AT5G60070	68.981	432	116	6	1	425	128	548	0.0	558
MS.gene028619.t1	AT5G02620	62.413	431	139	4	1	425	111	524	3.96e-176	503
MS.gene028619.t1	AT5G02620	62.413	431	139	4	1	425	111	524	3.96e-176	503
MS.gene028619.t1	AT5G02620	62.413	431	139	4	1	425	111	524	3.96e-176	503
MS.gene028619.t1	AT5G02620	59.251	454	139	5	1	425	111	547	7.42e-171	490
MS.gene028619.t1	AT5G02620	34.524	168	109	1	6	172	47	214	5.51e-17	83.6
MS.gene028619.t1	AT5G02620	59.251	454	139	5	1	425	111	547	7.42e-171	490
MS.gene028619.t1	AT5G02620	34.524	168	109	1	6	172	47	214	5.51e-17	83.6
MS.gene028619.t1	AT2G31820	59.953	427	161	5	1	425	244	662	8.73e-171	494
MS.gene028619.t1	AT1G05640	60.280	428	159	6	1	425	208	627	2.44e-167	484
MS.gene028619.t1	AT2G01680	46.500	400	198	4	1	394	114	503	2.89e-119	358
MS.gene028619.t1	AT3G12360	48.301	412	192	5	7	414	178	572	6.26e-119	358
MS.gene028619.t1	AT3G12360	48.301	412	192	5	7	414	190	584	9.14e-119	358
MS.gene028619.t1	AT3G09550	46.620	429	206	5	1	425	198	607	9.71e-110	336
MS.gene028619.t1	AT3G09550	46.620	429	206	5	1	425	198	607	9.71e-110	336
MS.gene028619.t1	AT3G09550	46.620	429	206	5	1	425	205	614	1.41e-109	335
MS.gene028619.t1	AT5G54710	29.078	423	275	12	1	411	228	637	2.44e-35	138
MS.gene028619.t1	AT5G54710	29.078	423	275	12	1	411	183	592	2.52e-35	138
MS.gene028619.t1	AT1G34050	26.209	393	259	8	1	385	170	539	1.78e-31	126
MS.gene028619.t1	AT1G34050	37.634	93	58	0	80	172	51	143	9.07e-11	64.3
MS.gene028619.t1	AT2G24600	25.485	412	262	15	1	381	152	549	1.28e-30	124
MS.gene028619.t1	AT2G24600	25.485	412	262	15	1	381	152	549	1.28e-30	124
MS.gene028619.t1	AT1G10340	25.238	420	283	12	1	396	148	560	5.07e-27	114
MS.gene028619.t1	AT1G10340	25.238	420	283	12	1	396	152	564	5.77e-27	113
MS.gene028619.t1	AT4G03460	27.027	296	172	8	17	306	267	524	7.81e-25	107
MS.gene028619.t1	AT4G03460	32.867	143	84	3	1	132	67	208	2.47e-11	66.2
MS.gene028619.t1	AT1G03670	25.000	364	207	8	17	349	251	579	8.97e-25	107
MS.gene028619.t1	AT4G03460	25.581	301	170	7	17	306	235	492	9.33e-25	107
MS.gene028619.t1	AT5G50140	23.246	499	267	11	1	403	47	525	1.53e-23	103
MS.gene028619.t1	AT4G03470	26.400	375	223	11	4	369	293	623	2.89e-20	94.0
MS.gene028619.t1	AT5G54700	24.925	333	192	8	41	368	23	302	3.07e-20	91.7
MS.gene028619.t1	AT4G03490	25.065	387	243	11	1	377	227	576	1.66e-19	91.3
MS.gene028619.t1	AT4G03490	25.065	387	243	11	1	377	217	566	1.78e-19	91.3
MS.gene028619.t1	AT4G03490	25.065	387	243	11	1	377	227	576	2.66e-19	90.9
MS.gene028619.t1	AT5G51160	23.925	372	222	9	11	338	43	397	7.86e-19	88.6
MS.gene028619.t1	AT4G14390	26.197	355	238	9	20	363	295	636	1.59e-18	88.6
MS.gene028619.t1	AT4G14390	26.197	355	238	9	20	363	276	617	2.17e-18	88.2
MS.gene028619.t1	AT4G14390	26.211	351	235	9	24	363	300	637	9.47e-18	86.3
MS.gene028619.t1	AT4G03480	26.433	314	182	6	5	306	377	653	1.03e-17	85.9
MS.gene028619.t1	AT4G03480	26.433	314	182	6	5	306	380	656	1.09e-17	85.9
MS.gene028619.t1	AT4G03500	23.398	359	250	8	19	364	251	597	1.59e-17	85.5
MS.gene028619.t1	AT4G03500	23.398	359	250	8	19	364	228	574	2.08e-17	85.1
MS.gene028619.t1	AT4G03500	23.398	359	250	8	19	364	228	574	2.08e-17	85.1
MS.gene028619.t1	AT4G10720	24.922	321	196	11	1	304	57	349	2.45e-17	84.0
MS.gene028619.t1	AT2G24600	25.862	290	183	10	1	266	152	433	7.51e-17	83.2
MS.gene028619.t1	AT2G24600	25.862	290	183	10	1	266	152	433	7.51e-17	83.2
MS.gene028619.t1	AT4G10720	26.715	277	166	9	1	261	57	312	1.70e-16	81.6
MS.gene028619.t1	AT4G05040	26.766	269	150	4	4	263	296	526	2.59e-16	81.6
MS.gene028619.t1	AT4G05040	26.766	269	150	4	4	263	296	526	2.59e-16	81.6
MS.gene028619.t1	AT4G05040	26.766	269	150	4	4	263	296	526	2.59e-16	81.6
MS.gene028619.t1	AT4G05040	26.766	269	150	4	4	263	296	526	2.59e-16	81.6
MS.gene028619.t1	AT4G05040	26.766	269	150	4	4	263	296	526	2.59e-16	81.6
MS.gene028619.t1	AT4G05040	26.766	269	150	4	4	263	296	526	2.59e-16	81.6
MS.gene028619.t1	AT4G05040	26.766	269	150	4	4	263	296	526	2.59e-16	81.6
MS.gene028619.t1	AT5G54700	23.919	347	199	9	46	368	34	339	8.44e-16	79.7
MS.gene028619.t1	AT3G04140	29.609	179	107	3	12	171	222	400	3.23e-15	78.2
MS.gene028619.t1	AT3G04140	38.318	107	64	1	18	122	192	298	3.64e-13	72.0
MS.gene028619.t1	AT3G04140	37.273	110	67	1	50	157	190	299	2.65e-11	65.9
MS.gene028619.t1	AT4G03490	21.760	409	234	11	11	377	154	518	1.05e-13	73.6
MS.gene028619.t1	AT4G03490	21.760	409	234	11	11	377	164	528	1.21e-13	73.6
MS.gene028619.t1	AT4G14390	26.984	252	167	6	20	260	276	521	4.26e-13	71.2
MS.gene028619.t1	AT5G15500	23.457	324	208	8	1	306	58	359	7.28e-13	70.5
MS.gene028619.t1	AT2G03430	30.682	176	115	5	19	192	51	221	3.06e-12	66.6
MS.gene028619.t1	AT4G14400	24.324	370	229	12	20	363	201	545	3.83e-12	68.6
MS.gene028619.t1	AT4G14400	24.324	370	229	12	20	363	201	545	3.83e-12	68.6
MS.gene028619.t1	AT4G14400	24.324	370	229	12	20	363	267	611	5.72e-12	68.2
MS.gene028619.t1	AT3G01750	27.950	161	100	3	12	156	222	382	2.20e-11	66.2
MS.gene028619.t1	AT3G01750	36.538	104	64	1	52	153	192	295	3.44e-11	65.9
MS.gene028619.t1	AT3G01750	27.950	161	100	3	12	156	222	382	2.20e-11	66.2
MS.gene028619.t1	AT3G01750	36.538	104	64	1	52	153	192	295	3.44e-11	65.9
MS.gene028619.t1	AT5G07840	40.196	102	57	2	6	103	22	123	2.61e-11	62.4
MS.gene028619.t1	AT1G14500	30.709	127	86	2	13	138	35	160	3.35e-11	65.1
MS.gene028619.t1	AT5G54610	26.316	190	123	4	5	177	25	214	4.90e-11	64.7

Find 81 sgRNAs with CRISPR-Local

Find 100 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
CTTTGGGAACTATGTGTTAT+TGG	0.157747	2.3:+6342236	MS.gene028619:CDS
TGCAGCAAGGACTGATAAAA+AGG	0.264101	2.3:+6341094	MS.gene028619:CDS
AACTATAATGGCTACAACTT+TGG	0.277317	2.3:+6342219	MS.gene028619:CDS
AAAATTATAAATGGTGCTTC+TGG	0.278164	2.3:-6341971	None:intergenic
TACCCGAGTTCTGGCTTTCC+TGG	0.287238	2.3:-6341243	None:intergenic
GTAGACACTGCTGAGAAAAT+AGG	0.288492	2.3:+6341587	MS.gene028619:CDS
CAGATTGTAAATATGCTTCT+AGG	0.300619	2.3:+6341515	MS.gene028619:intron
ATCCCTTCTGGAATATTGTT+TGG	0.316664	2.3:-6341923	None:intergenic
TATTGCACTCTTCATTTCTC+TGG	0.325254	2.3:+6342009	MS.gene028619:CDS
AATACAAAGAAAATTATAAA+TGG	0.331303	2.3:-6341980	None:intergenic
TATGTAATTTACCCGAGTTC+TGG	0.335480	2.3:-6341252	None:intergenic
TGCAAAGCTGTTGTGTTTGA+TGG	0.339635	2.3:-6340906	None:intergenic
TTTGGGAACTATGTGTTATT+GGG	0.342224	2.3:+6342237	MS.gene028619:CDS
TGAGATAGTGAAATTCCTAT+TGG	0.358809	2.3:+6340953	MS.gene028619:CDS
GCATTGTCATTTGTTGTTGT+TGG	0.360348	2.3:+6342151	MS.gene028619:CDS
CTTCATTCTGCTGCAAGAAA+TGG	0.361031	2.3:+6341026	MS.gene028619:CDS
TCCACTGTCATTGACAATTC+AGG	0.372401	2.3:-6340882	None:intergenic
CATCAAACTTGAGGAACATT+AGG	0.373191	2.3:+6342281	MS.gene028619:CDS
ACTATAATGGCTACAACTTT+GGG	0.376333	2.3:+6342220	MS.gene028619:CDS
TCAACCATGTTTATTGTTGA+AGG	0.380744	2.3:-6341188	None:intergenic
TCACCAAGTATCATCCCTTC+TGG	0.381269	2.3:-6341935	None:intergenic
TCAGTCCTTGCTGCAACTCC+TGG	0.385882	2.3:-6341086	None:intergenic
GAACACCATGCTCTAATAGA+AGG	0.390495	2.3:-6341628	None:intergenic
CATTATAGTTGCTCCTATAA+TGG	0.392151	2.3:-6342206	None:intergenic
TGCTGCAAGAAATGGACATT+TGG	0.400973	2.3:+6341034	MS.gene028619:CDS
TCTGGCGTGTGTGTTCTAAT+TGG	0.405927	2.3:-6341745	None:intergenic
CATTATAGGAGCAACTATAA+TGG	0.420585	2.3:+6342207	MS.gene028619:CDS
ATTGGAAGCAGGAAGTAGTT+TGG	0.421802	2.3:+6340971	MS.gene028619:CDS
CTTCATTTCTCTGGCTGTTG+TGG	0.427415	2.3:+6342018	MS.gene028619:CDS
TAGACACTGCTGAGAAAATA+GGG	0.434367	2.3:+6341588	MS.gene028619:CDS
CTGCCATGTGAAGTGCTGTC+TGG	0.436714	2.3:-6341118	None:intergenic
GCACTTCTTGAGAAAGAGCC+AGG	0.460376	2.3:+6341068	MS.gene028619:CDS
GTAAGTGCTGTTAACAGATC+AGG	0.461810	2.3:+6341554	MS.gene028619:CDS
TGCTAAAAGTAACGGGAAAA+CGG	0.468457	2.3:+6341001	MS.gene028619:CDS
GAACATTAGGAAATCTTCAA+TGG	0.470623	2.3:+6342294	MS.gene028619:CDS
AAATATGCTTCTAGGACAAA+AGG	0.471296	2.3:+6341523	MS.gene028619:CDS
AGCACTTCACATGGCAGTAA+AGG	0.471764	2.3:+6341124	MS.gene028619:CDS
CAGACACGAAAACGTGTTCA+AGG	0.474794	2.3:+6341764	MS.gene028619:CDS
GGGACAAAATCTTGAGGTAG+TGG	0.477594	2.3:+6341145	MS.gene028619:CDS
CAACCATTGCTAAAAGTAAC+GGG	0.485668	2.3:+6340994	MS.gene028619:CDS
GCACTTCACATGGCAGTAAA+GGG	0.488917	2.3:+6341125	MS.gene028619:CDS
AACTACTTCCTGCTTCCAAT+AGG	0.491463	2.3:-6340968	None:intergenic
AATTAATAAGCTAATGTGGT+TGG	0.495846	2.3:+6342099	MS.gene028619:CDS
GGCTTGTGTGCTTATTTCTG+TGG	0.502072	2.3:+6342120	MS.gene028619:CDS
GTGAAATTCCTATTGGAAGC+AGG	0.510342	2.3:+6340960	MS.gene028619:CDS
ATTCCAGAAGGGATGATACT+TGG	0.510792	2.3:+6341932	MS.gene028619:CDS
CAACCAGGAAAGCCAGAACT+CGG	0.516470	2.3:+6341240	MS.gene028619:CDS
ATAGAAGGGCTTTAACATCC+TGG	0.524388	2.3:-6341613	None:intergenic
GCAACCATTGCTAAAAGTAA+CGG	0.526222	2.3:+6340993	MS.gene028619:CDS
ATAAACATGGTTGATACTAA+AGG	0.532093	2.3:+6341197	MS.gene028619:CDS
GGTCATTAAGCATCGAATTG+AGG	0.539272	2.3:+6342258	MS.gene028619:CDS
TGCAAGAAATGGACATTTGG+AGG	0.543958	2.3:+6341037	MS.gene028619:CDS
GTCATTTGTTGTTGTTGGAA+AGG	0.554079	2.3:+6342156	MS.gene028619:CDS
GTGCTTGTTGATCCTTGTGG+TGG	0.555571	2.3:-6341671	None:intergenic
AAGCAAAGCGAAGAAACAAA+TGG	0.557199	2.3:+6342069	MS.gene028619:CDS
TGTTGTGGTTGTTCAAACTT+CGG	0.558193	2.3:+6342033	MS.gene028619:CDS
GATCCAAACAATATTCCAGA+AGG	0.558398	2.3:+6341920	MS.gene028619:CDS
TGCTGAGAAAATAGGGAACC+AGG	0.569656	2.3:+6341595	MS.gene028619:CDS
GGATCATCAACAAATTGACC+AGG	0.588310	2.3:-6341902	None:intergenic
AACACCATGCTCTAATAGAA+GGG	0.595108	2.3:-6341627	None:intergenic
TGCTATTAGTACTGCCACAA+CGG	0.600090	2.3:-6341849	None:intergenic
AACCAGGAAAGCCAGAACTC+GGG	0.603570	2.3:+6341241	MS.gene028619:CDS
CTATAATTAATAAGCTAATG+TGG	0.603869	2.3:+6342095	MS.gene028619:CDS
AAAGCCCTTCTATTAGAGCA+TGG	0.609187	2.3:+6341623	MS.gene028619:CDS
TCCTGAATTGTCAATGACAG+TGG	0.613702	2.3:+6340881	MS.gene028619:CDS
ATCAACAAGATGAATACAGA+AGG	0.614257	2.3:+6341800	MS.gene028619:CDS
GGGCCAGACAGCACTTCACA+TGG	0.614969	2.3:+6341115	MS.gene028619:CDS
AAGAGCCAGGAGTTGCAGCA+AGG	0.615235	2.3:+6341081	MS.gene028619:CDS
CAACATTGCATATAGCAACC+AGG	0.627104	2.3:+6341225	MS.gene028619:CDS
TTGCATACAGCTGCAACACA+AGG	0.627719	2.3:+6340924	MS.gene028619:CDS
GAATTGAGGCATCAAACTTG+AGG	0.637621	2.3:+6342272	MS.gene028619:CDS
ATCCAAACAATATTCCAGAA+GGG	0.645443	2.3:+6341921	MS.gene028619:CDS
AGATCCTTCAACAATAAACA+TGG	0.658313	2.3:+6341184	MS.gene028619:CDS
TGTGGCAGTACTAATAGCAA+CGG	0.666243	2.3:+6341853	MS.gene028619:CDS
AATAAACTCGACAACCGTTG+TGG	0.669177	2.3:+6341835	MS.gene028619:CDS
AGTAAAGGGACAAAATCTTG+AGG	0.673310	2.3:+6341139	MS.gene028619:CDS
GCTGTGCTTGTTGATCCTTG+TGG	0.678811	2.3:-6341674	None:intergenic
GATCATCAACAAATTGACCA+GGG	0.681393	2.3:-6341901	None:intergenic
CAAAGCGAAGAAACAAATGG+CGG	0.712434	2.3:+6342072	MS.gene028619:CDS
AAGTCTATAAAACCACCACA+AGG	0.715719	2.3:+6341659	MS.gene028619:CDS
TGTAAGCGACATAAAGCACG+AGG	0.760936	2.3:+6341715	MS.gene028619:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	AATACAAAGAAAATTATAAA+TGG	-	chr2.3:6341983-6342002	None:intergenic	10.0%
!!!	AATTATTTGAATTTGTTTTC+TGG	+	chr2.3:6341329-6341348	MS.gene028619:intron	15.0%
!!	CTATAATTAATAAGCTAATG+TGG	+	chr2.3:6342095-6342114	MS.gene028619:CDS	20.0%
!	AAAATTATAAATGGTGCTTC+TGG	-	chr2.3:6341974-6341993	None:intergenic	25.0%
!	AAATTCCATAGACATATACT+AGG	+	chr2.3:6341285-6341304	MS.gene028619:intron	25.0%
!	AATTAATAAGCTAATGTGGT+TGG	+	chr2.3:6342099-6342118	MS.gene028619:CDS	25.0%
!!	ATAAACATGGTTGATACTAA+AGG	+	chr2.3:6341197-6341216	MS.gene028619:CDS	25.0%
!!	GCAATTTTTGTTACCATTAT+AGG	+	chr2.3:6342193-6342212	MS.gene028619:CDS	25.0%
	AACTATAATGGCTACAACTT+TGG	+	chr2.3:6342219-6342238	MS.gene028619:CDS	30.0%
	ACTATAATGGCTACAACTTT+GGG	+	chr2.3:6342220-6342239	MS.gene028619:CDS	30.0%
	AGATCCTTCAACAATAAACA+TGG	+	chr2.3:6341184-6341203	MS.gene028619:CDS	30.0%
	ATAGACATATACTAGGTACT+AGG	+	chr2.3:6341292-6341311	MS.gene028619:intron	30.0%
	ATCAACAAGATGAATACAGA+AGG	+	chr2.3:6341800-6341819	MS.gene028619:CDS	30.0%
	ATCCAAACAATATTCCAGAA+GGG	+	chr2.3:6341921-6341940	MS.gene028619:CDS	30.0%
	CAGATTGTAAATATGCTTCT+AGG	+	chr2.3:6341515-6341534	MS.gene028619:intron	30.0%
	CATTATAGGAGCAACTATAA+TGG	+	chr2.3:6342207-6342226	MS.gene028619:CDS	30.0%
	CATTATAGTTGCTCCTATAA+TGG	-	chr2.3:6342209-6342228	None:intergenic	30.0%
	GAACATTAGGAAATCTTCAA+TGG	+	chr2.3:6342294-6342313	MS.gene028619:CDS	30.0%
	TAGACATATACTAGGTACTA+GGG	+	chr2.3:6341293-6341312	MS.gene028619:intron	30.0%
	TAGTACCTAGTATATGTCTA+TGG	-	chr2.3:6341293-6341312	None:intergenic	30.0%
	TCAACCATGTTTATTGTTGA+AGG	-	chr2.3:6341191-6341210	None:intergenic	30.0%
	TGAGATAGTGAAATTCCTAT+TGG	+	chr2.3:6340953-6340972	MS.gene028619:CDS	30.0%
	TTGTTGGAAAGGAAGAAAAA+TGG	+	chr2.3:6342167-6342186	MS.gene028619:CDS	30.0%
!	TTTGGGAACTATGTGTTATT+GGG	+	chr2.3:6342237-6342256	MS.gene028619:CDS	30.0%
!!	AAATATGCTTCTAGGACAAA+AGG	+	chr2.3:6341523-6341542	MS.gene028619:CDS	30.0%
	AACACCATGCTCTAATAGAA+GGG	-	chr2.3:6341630-6341649	None:intergenic	35.0%
	AAGCAAAGCGAAGAAACAAA+TGG	+	chr2.3:6342069-6342088	MS.gene028619:CDS	35.0%
	AAGTCTATAAAACCACCACA+AGG	+	chr2.3:6341659-6341678	MS.gene028619:CDS	35.0%
	AAGTTGCAGAAAGTAGCTTA+TGG	-	chr2.3:6341410-6341429	None:intergenic	35.0%
	AGTAAAGGGACAAAATCTTG+AGG	+	chr2.3:6341139-6341158	MS.gene028619:CDS	35.0%
	CAACCATTGCTAAAAGTAAC+GGG	+	chr2.3:6340994-6341013	MS.gene028619:CDS	35.0%
	CATCAAACTTGAGGAACATT+AGG	+	chr2.3:6342281-6342300	MS.gene028619:CDS	35.0%
	GATCATCAACAAATTGACCA+GGG	-	chr2.3:6341904-6341923	None:intergenic	35.0%
	GATCCAAACAATATTCCAGA+AGG	+	chr2.3:6341920-6341939	MS.gene028619:CDS	35.0%
	GCAACCATTGCTAAAAGTAA+CGG	+	chr2.3:6340993-6341012	MS.gene028619:CDS	35.0%
	TAGACACTGCTGAGAAAATA+GGG	+	chr2.3:6341588-6341607	MS.gene028619:CDS	35.0%
	TATGTAATTTACCCGAGTTC+TGG	-	chr2.3:6341255-6341274	None:intergenic	35.0%
	TATTGCACTCTTCATTTCTC+TGG	+	chr2.3:6342009-6342028	MS.gene028619:CDS	35.0%
	TGGAAAGGAAGAAAAATGGT+TGG	+	chr2.3:6342171-6342190	MS.gene028619:CDS	35.0%
	TGTTGTGGTTGTTCAAACTT+CGG	+	chr2.3:6342033-6342052	MS.gene028619:CDS	35.0%
	TTGAAATCCGTCATTGACAA+TGG	+	chr2.3:6341434-6341453	MS.gene028619:intron	35.0%
!	CTTTGGGAACTATGTGTTAT+TGG	+	chr2.3:6342236-6342255	MS.gene028619:CDS	35.0%
!	TGCTAAAAGTAACGGGAAAA+CGG	+	chr2.3:6341001-6341020	MS.gene028619:CDS	35.0%
!!	ATCCCTTCTGGAATATTGTT+TGG	-	chr2.3:6341926-6341945	None:intergenic	35.0%
!!	GCATTGTCATTTGTTGTTGT+TGG	+	chr2.3:6342151-6342170	MS.gene028619:CDS	35.0%
!!	GTCATTTGTTGTTGTTGGAA+AGG	+	chr2.3:6342156-6342175	MS.gene028619:CDS	35.0%
!!	TAAGCACTTCTGAAACTGTT+TGG	+	chr2.3:6341354-6341373	MS.gene028619:intron	35.0%
!!	TATTGACATCTGTTTCTGAG+AGG	+	chr2.3:6341468-6341487	MS.gene028619:intron	35.0%
!!!	TTTCCCGTTACTTTTAGCAA+TGG	-	chr2.3:6341000-6341019	None:intergenic	35.0%
	AAAGCCCTTCTATTAGAGCA+TGG	+	chr2.3:6341623-6341642	MS.gene028619:CDS	40.0%
	AACTACTTCCTGCTTCCAAT+AGG	-	chr2.3:6340971-6340990	None:intergenic	40.0%
	AATAAACTCGACAACCGTTG+TGG	+	chr2.3:6341835-6341854	MS.gene028619:CDS	40.0%
	ATCCGTCATTGACAATGGAA+AGG	+	chr2.3:6341439-6341458	MS.gene028619:intron	40.0%
	ATTCCAGAAGGGATGATACT+TGG	+	chr2.3:6341932-6341951	MS.gene028619:CDS	40.0%
	ATTGGAAGCAGGAAGTAGTT+TGG	+	chr2.3:6340971-6340990	MS.gene028619:CDS	40.0%
	CAAAGCGAAGAAACAAATGG+CGG	+	chr2.3:6342072-6342091	MS.gene028619:CDS	40.0%
	CAACATTGCATATAGCAACC+AGG	+	chr2.3:6341225-6341244	MS.gene028619:CDS	40.0%
	CTTCATTCTGCTGCAAGAAA+TGG	+	chr2.3:6341026-6341045	MS.gene028619:CDS	40.0%
	GAACACCATGCTCTAATAGA+AGG	-	chr2.3:6341631-6341650	None:intergenic	40.0%
	GCAGCAAGGACTGATAAAAA+GGG	+	chr2.3:6341095-6341114	MS.gene028619:CDS	40.0%
	GGATCATCAACAAATTGACC+AGG	-	chr2.3:6341905-6341924	None:intergenic	40.0%
	GTAGACACTGCTGAGAAAAT+AGG	+	chr2.3:6341587-6341606	MS.gene028619:CDS	40.0%
	GTGAAATTCCTATTGGAAGC+AGG	+	chr2.3:6340960-6340979	MS.gene028619:CDS	40.0%
	TCCACTGTCATTGACAATTC+AGG	-	chr2.3:6340885-6340904	None:intergenic	40.0%
	TCCCTTTCCATTGTCAATGA+CGG	-	chr2.3:6341444-6341463	None:intergenic	40.0%
	TCCGTCATTGACAATGGAAA+GGG	+	chr2.3:6341440-6341459	MS.gene028619:intron	40.0%
	TCCTGAATTGTCAATGACAG+TGG	+	chr2.3:6340881-6340900	MS.gene028619:CDS	40.0%
	TGCAAAGCTGTTGTGTTTGA+TGG	-	chr2.3:6340909-6340928	None:intergenic	40.0%
	TGCAAGAAATGGACATTTGG+AGG	+	chr2.3:6341037-6341056	MS.gene028619:CDS	40.0%
	TGCAGCAAGGACTGATAAAA+AGG	+	chr2.3:6341094-6341113	MS.gene028619:CDS	40.0%
	TGCTATTAGTACTGCCACAA+CGG	-	chr2.3:6341852-6341871	None:intergenic	40.0%
	TGCTGCAAGAAATGGACATT+TGG	+	chr2.3:6341034-6341053	MS.gene028619:CDS	40.0%
	TGTGGCAGTACTAATAGCAA+CGG	+	chr2.3:6341853-6341872	MS.gene028619:CDS	40.0%
!	ATAGAAGGGCTTTAACATCC+TGG	-	chr2.3:6341616-6341635	None:intergenic	40.0%
!	GAATTGAGGCATCAAACTTG+AGG	+	chr2.3:6342272-6342291	MS.gene028619:CDS	40.0%
!	GTAAGTGCTGTTAACAGATC+AGG	+	chr2.3:6341554-6341573	MS.gene028619:CDS	40.0%
!!	GGTCATTAAGCATCGAATTG+AGG	+	chr2.3:6342258-6342277	MS.gene028619:CDS	40.0%
	AGCACTTCACATGGCAGTAA+AGG	+	chr2.3:6341124-6341143	MS.gene028619:CDS	45.0%
	CAGACACGAAAACGTGTTCA+AGG	+	chr2.3:6341764-6341783	MS.gene028619:CDS	45.0%
	CTTCATTTCTCTGGCTGTTG+TGG	+	chr2.3:6342018-6342037	MS.gene028619:CDS	45.0%
	GCACTTCACATGGCAGTAAA+GGG	+	chr2.3:6341125-6341144	MS.gene028619:CDS	45.0%
	GGGACAAAATCTTGAGGTAG+TGG	+	chr2.3:6341145-6341164	MS.gene028619:CDS	45.0%
	TCACCAAGTATCATCCCTTC+TGG	-	chr2.3:6341938-6341957	None:intergenic	45.0%
	TCTGGCGTGTGTGTTCTAAT+TGG	-	chr2.3:6341748-6341767	None:intergenic	45.0%
	TGCTGAGAAAATAGGGAACC+AGG	+	chr2.3:6341595-6341614	MS.gene028619:CDS	45.0%
	TGTAAGCGACATAAAGCACG+AGG	+	chr2.3:6341715-6341734	MS.gene028619:CDS	45.0%
	TTGCATACAGCTGCAACACA+AGG	+	chr2.3:6340924-6340943	MS.gene028619:CDS	45.0%
!	CTTGAACACGTTTTCGTGTC+TGG	-	chr2.3:6341766-6341785	None:intergenic	45.0%
!!	GGCTTGTGTGCTTATTTCTG+TGG	+	chr2.3:6342120-6342139	MS.gene028619:CDS	45.0%
	AACCAGGAAAGCCAGAACTC+GGG	+	chr2.3:6341241-6341260	MS.gene028619:CDS	50.0%
	CAACCAGGAAAGCCAGAACT+CGG	+	chr2.3:6341240-6341259	MS.gene028619:CDS	50.0%
	GCACTTCTTGAGAAAGAGCC+AGG	+	chr2.3:6341068-6341087	MS.gene028619:CDS	50.0%
!	GCTGTGCTTGTTGATCCTTG+TGG	-	chr2.3:6341677-6341696	None:intergenic	50.0%
!	GTGCTTGTTGATCCTTGTGG+TGG	-	chr2.3:6341674-6341693	None:intergenic	50.0%
	AAGAGCCAGGAGTTGCAGCA+AGG	+	chr2.3:6341081-6341100	MS.gene028619:CDS	55.0%
	TCAGTCCTTGCTGCAACTCC+TGG	-	chr2.3:6341089-6341108	None:intergenic	55.0%
!	GCTGCGATTTTCACTGTCCC+TGG	+	chr2.3:6341884-6341903	MS.gene028619:CDS	55.0%
!!	CTGCCATGTGAAGTGCTGTC+TGG	-	chr2.3:6341121-6341140	None:intergenic	55.0%
!!	TACCCGAGTTCTGGCTTTCC+TGG	-	chr2.3:6341246-6341265	None:intergenic	55.0%
	GGGCCAGACAGCACTTCACA+TGG	+	chr2.3:6341115-6341134	MS.gene028619:CDS	60.0%

Chromosome	Type	Strat	End	Strand	Name
chr2.3	gene	6340870	6342402	6340870	ID=MS.gene028619
chr2.3	mRNA	6340870	6342402	6340870	ID=MS.gene028619.t1;Parent=MS.gene028619
chr2.3	exon	6340870	6341262	6340870	ID=MS.gene028619.t1.exon1;Parent=MS.gene028619.t1
chr2.3	CDS	6340870	6341262	6340870	ID=cds.MS.gene028619.t1;Parent=MS.gene028619.t1
chr2.3	exon	6341518	6342402	6341518	ID=MS.gene028619.t1.exon2;Parent=MS.gene028619.t1
chr2.3	CDS	6341518	6342402	6341518	ID=cds.MS.gene028619.t1;Parent=MS.gene028619.t1

Gene Sequence

>MS.gene028619

ATGGAGGTTCATCCTGAATTGTCAATGACAGTGGATCCATCAAACACAACAGCTTTGCATACAGCTGCAACACAAGGACATATTGAGATAGTGAAATTCCTATTGGAAGCAGGAAGTAGTTTGGCAACCATTGCTAAAAGTAACGGGAAAACGGCTCTTCATTCTGCTGCAAGAAATGGACATTTGGAGGTTGTGAAAGCACTTCTTGAGAAAGAGCCAGGAGTTGCAGCAAGGACTGATAAAAAGGGCCAGACAGCACTTCACATGGCAGTAAAGGGACAAAATCTTGAGGTAGTGGAAGAGTTGATAAAAGCAGATCCTTCAACAATAAACATGGTTGATACTAAAGGAAACACAACATTGCATATAGCAACCAGGAAAGCCAGAACTCGGGTAAATTACATAATGCTTGTCAAAATTCCATAGACATATACTAGGTACTAGGGTCTGTTTGTATTGAATTATTTGAATTTGTTTTCTGGCATAAGCACTTCTGAAACTGTTTGGAAGTGCTTGTGAAAACAGCTTATGACACATCCATAAGCTACTTTCTGCAACTTAATTTTGAAATCCGTCATTGACAATGGAAAGGGAAAATTATTGACATCTGTTTCTGAGAGGCTAAAATATGTAAATTTGTTTGTGCAGATTGTAAATATGCTTCTAGGACAAAAGGAAACAAATGTAAGTGCTGTTAACAGATCAGGTGAAACAGCAGTAGACACTGCTGAGAAAATAGGGAACCAGGATGTTAAAGCCCTTCTATTAGAGCATGGTGTTCAGAGTGCAAAGTCTATAAAACCACCACAAGGATCAACAAGCACAGCTCGCGAGTTAAAGCAAACTGTAAGCGACATAAAGCACGAGGTACATTACCAATTAGAACACACACGCCAGACACGAAAACGTGTTCAAGGAATCGCAAAACGTATCAACAAGATGAATACAGAAGGACTCAACAATGCAATAAACTCGACAACCGTTGTGGCAGTACTAATAGCAACGGTTGCATTTGCTGCGATTTTCACTGTCCCTGGTCAATTTGTTGATGATCCAAACAATATTCCAGAAGGGATGATACTTGGTGAAGCAAATATATCTCCAGAAGCACCATTTATAATTTTCTTTGTATTTGATTCTATTGCACTCTTCATTTCTCTGGCTGTTGTGGTTGTTCAAACTTCGGTTGTCGTTATAGAAAGCAAAGCGAAGAAACAAATGGCGGCTATAATTAATAAGCTAATGTGGTTGGCTTGTGTGCTTATTTCTGTGGCATTTTTAGCATTGTCATTTGTTGTTGTTGGAAAGGAAGAAAAATGGTTGGCAATTTTTGTTACCATTATAGGAGCAACTATAATGGCTACAACTTTGGGAACTATGTGTTATTGGGTCATTAAGCATCGAATTGAGGCATCAAACTTGAGGAACATTAGGAAATCTTCAATGGAAAGCAAATCAAAGTCTTTTTCAGTGACAGCTTTGTCAGATTCTGAGCTAATGAACAATGAATTTAAGAAAATGTATGCTATTTAG

Protein sequence

>MS.gene028619.t1

MEVHPELSMTVDPSNTTALHTAATQGHIEIVKFLLEAGSSLATIAKSNGKTALHSAARNGHLEVVKALLEKEPGVAARTDKKGQTALHMAVKGQNLEVVEELIKADPSTINMVDTKGNTTLHIATRKARTRIVNMLLGQKETNVSAVNRSGETAVDTAEKIGNQDVKALLLEHGVQSAKSIKPPQGSTSTARELKQTVSDIKHEVHYQLEHTRQTRKRVQGIAKRINKMNTEGLNNAINSTTVVAVLIATVAFAAIFTVPGQFVDDPNNIPEGMILGEANISPEAPFIIFFVFDSIALFISLAVVVVQTSVVVIESKAKKQMAAIINKLMWLACVLISVAFLALSFVVVGKEEKWLAIFVTIIGATIMATTLGTMCYWVIKHRIEASNLRNIRKSSMESKSKSFSVTALSDSELMNNEFKKMYAI