Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene38996.t1 | XP_003628452.2 | 99.6 | 523 | 2 | 0 | 1 | 523 | 17 | 539 | 1.40E-304 | 1054.7 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene38996.t1 | B3DNN5 | 75.0 | 529 | 123 | 5 | 1 | 523 | 17 | 542 | 2.1e-219 | 763.1 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene38996.t1 | D0VFU6 | 99.6 | 523 | 2 | 0 | 1 | 523 | 17 | 539 | 1.0e-304 | 1054.7 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene38996.t1 | MTR_8g058380 | 99.618 | 523 | 2 | 0 | 1 | 523 | 17 | 539 | 0.0 | 1086 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene38996.t1 | AT1G78770 | 77.127 | 529 | 112 | 5 | 1 | 523 | 17 | 542 | 0.0 | 809 |
Find 116 sgRNAs with CRISPR-Local
Find 0 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GAAAACCAATAATGATTTAT+TGG | 0.178881 | 8.4:+48660977 | MS.gene38996:CDS |
| GCAGGCCAATAAATCATTAT+TGG | 0.215518 | 8.4:-48660982 | None:intergenic |
| TGCATATACTTACCACTTAC+AGG | 0.260832 | 8.4:+48665045 | MS.gene38996:CDS |
| TTGATTTCGTCATTGCAATT+TGG | 0.265331 | 8.4:+48660725 | MS.gene38996:CDS |
| TCCTTACTCTTTCATATAAT+AGG | 0.285237 | 8.4:-48662795 | None:intergenic |
| CACTTACCAGCTCTTTATAT+TGG | 0.295284 | 8.4:+48662407 | MS.gene38996:CDS |
| GGTGAAAGTGGCAACTTTAT+CGG | 0.303029 | 8.4:-48653004 | None:intergenic |
| CAGCTATAAGCTTGCTGAAC+AGG | 0.316612 | 8.4:+48662451 | MS.gene38996:CDS |
| ACTGCAGCTCGATTATTTCC+TGG | 0.319914 | 8.4:+48661845 | MS.gene38996:CDS |
| ATCTTTATCCAAGTAGATAT+TGG | 0.322898 | 8.4:-48653250 | None:intergenic |
| GATTCCACTCGTTCAATTCC+TGG | 0.326570 | 8.4:-48653164 | None:intergenic |
| GTACTAATGTGCTCTTGATA+TGG | 0.332010 | 8.4:-48661220 | None:intergenic |
| ATGATAGTATTCAGCTTTGC+AGG | 0.345674 | 8.4:-48661000 | None:intergenic |
| GAGGTCACGGAGGACGATTT+TGG | 0.378773 | 8.4:-48653112 | None:intergenic |
| ATCCCCACCTTGAACTCTAA+TGG | 0.387146 | 8.4:+48666799 | MS.gene38996:CDS |
| CATAATATGAAATTGCTTCT+CGG | 0.388953 | 8.4:-48664976 | None:intergenic |
| CTGGCAGCTGCTATGGAGCT+TGG | 0.390830 | 8.4:+48661245 | MS.gene38996:CDS |
| CAAGAGCACATTAGTACATC+TGG | 0.399689 | 8.4:+48661226 | MS.gene38996:CDS |
| GTTAAATTTACTTGAAATAA+CGG | 0.399730 | 8.4:-48661537 | None:intergenic |
| CACTTAAAGCGGTAGGGATG+AGG | 0.421874 | 8.4:-48663870 | None:intergenic |
| GGTGGAAGGCGCGGCGGTAA+TGG | 0.429908 | 8.4:-48653077 | None:intergenic |
| ACTTGAAATAACGGCGGGAT+TGG | 0.433856 | 8.4:-48661528 | None:intergenic |
| CGCGGCGGTAATGGCGGCCG+AGG | 0.434092 | 8.4:-48653068 | None:intergenic |
| AATGTGTACCTCATAGCATA+AGG | 0.443763 | 8.4:-48659036 | None:intergenic |
| AGTACATCTGGCAGCTGCTA+TGG | 0.448725 | 8.4:+48661238 | MS.gene38996:CDS |
| TGGCAGCTGCTATGGAGCTT+GGG | 0.450830 | 8.4:+48661246 | MS.gene38996:CDS |
| TCAGTGCAGAACTGATCATC+TGG | 0.451911 | 8.4:-48666732 | None:intergenic |
| TTCCCATAGCCTATCCATGC+AGG | 0.454837 | 8.4:-48661773 | None:intergenic |
| TAATGCCGCCAATATCTACT+TGG | 0.459731 | 8.4:+48653242 | MS.gene38996:CDS |
| TTATTCTTGTTTGATTAAGA+AGG | 0.461366 | 8.4:+48660775 | MS.gene38996:CDS |
| GGCACTGTGCAGCGAGGTAG+CGG | 0.462555 | 8.4:-48653140 | None:intergenic |
| AGCTAAAGAAGAGGGTGATC+AGG | 0.462934 | 8.4:+48661805 | MS.gene38996:CDS |
| CTGCAGCTCGATTATTTCCT+GGG | 0.463353 | 8.4:+48661846 | MS.gene38996:CDS |
| TTCAGTGTGCGGAAGAAAGA+AGG | 0.467284 | 8.4:-48660957 | None:intergenic |
| TGAGCAATATTGACTAAAGT+TGG | 0.467691 | 8.4:-48663904 | None:intergenic |
| AGCTCTAGAAAACCGTTCTC+AGG | 0.482124 | 8.4:+48658765 | MS.gene38996:CDS |
| AGCAGGATCGTTGGTGAAAG+TGG | 0.483776 | 8.4:-48653016 | None:intergenic |
| TGCTCATGCATGCAGAAAGT+TGG | 0.488077 | 8.4:+48663921 | MS.gene38996:CDS |
| TGTTGTTTAGGGCTTCATCA+TGG | 0.488358 | 8.4:+48661464 | MS.gene38996:intron |
| TCTGCACTGAAATGTTAAGC+TGG | 0.488942 | 8.4:+48666745 | MS.gene38996:CDS |
| GTGCAGCGAGGTAGCGGAAG+CGG | 0.493534 | 8.4:-48653134 | None:intergenic |
| CCACTTGTGTATAACGAACT+AGG | 0.501430 | 8.4:+48662764 | MS.gene38996:CDS |
| ACCAGCTCTTTATATTGGAA+TGG | 0.501493 | 8.4:+48662412 | MS.gene38996:CDS |
| GATAAATCCATTAGAGTTCA+AGG | 0.502009 | 8.4:-48666806 | None:intergenic |
| TTCAGCAAGCTTATAGCTGT+GGG | 0.502131 | 8.4:-48662447 | None:intergenic |
| GCTTCATCATGGTTTGCTGT+TGG | 0.502404 | 8.4:+48661475 | MS.gene38996:CDS |
| CCTCGCTGCACAGTGCCTCC+AGG | 0.505148 | 8.4:+48653146 | MS.gene38996:CDS |
| CACATGCTTACATGTGATGA+AGG | 0.505528 | 8.4:+48660557 | MS.gene38996:CDS |
| TTTAACCTCCTCACCGAGCA+TGG | 0.511312 | 8.4:-48653196 | None:intergenic |
| ATATTACAGTTTGCTTGAGA+AGG | 0.520023 | 8.4:+48661190 | MS.gene38996:intron |
| CATGTTTCACTTAAAGCGGT+AGG | 0.524123 | 8.4:-48663877 | None:intergenic |
| GTTCTCAAGATCTCTAAACT+TGG | 0.526440 | 8.4:-48660907 | None:intergenic |
| ATCAAATGAACTTTATCTGA+TGG | 0.529595 | 8.4:+48661271 | MS.gene38996:CDS |
| ACAAGTTTGAGCACTTATGC+TGG | 0.530284 | 8.4:+48665019 | MS.gene38996:CDS |
| CCCCTGCATGGATAGGCTAT+GGG | 0.540578 | 8.4:+48661771 | MS.gene38996:CDS |
| GTCATTGCAATTTGGAAGTG+AGG | 0.540735 | 8.4:+48660733 | MS.gene38996:CDS |
| AGCGGATCCCTTATGCTATG+AGG | 0.542168 | 8.4:+48659028 | MS.gene38996:CDS |
| TCCATTCCAATATAAAGAGC+TGG | 0.542867 | 8.4:-48662413 | None:intergenic |
| GTTCAGCAAGCTTATAGCTG+TGG | 0.543435 | 8.4:-48662448 | None:intergenic |
| GGAGGCGTTGAGGAGGTGGA+AGG | 0.544257 | 8.4:-48653091 | None:intergenic |
| CTGCACTGAAATGTTAAGCT+GGG | 0.545603 | 8.4:+48666746 | MS.gene38996:CDS |
| GGAAGGCGCGGCGGTAATGG+CGG | 0.546984 | 8.4:-48653074 | None:intergenic |
| ATGTGTACCTCATAGCATAA+GGG | 0.547549 | 8.4:-48659035 | None:intergenic |
| TTGCAATTTGGAAGTGAGGA+TGG | 0.551069 | 8.4:+48660737 | MS.gene38996:CDS |
| CAATATAAAGAGCTGGTAAG+TGG | 0.552656 | 8.4:-48662406 | None:intergenic |
| CTCCCATGTTTCACTTAAAG+CGG | 0.554446 | 8.4:-48663881 | None:intergenic |
| AATTTACTTGAAATAACGGC+GGG | 0.554608 | 8.4:-48661533 | None:intergenic |
| CTACCGCTTTAAGTGAAACA+TGG | 0.558823 | 8.4:+48663878 | MS.gene38996:CDS |
| CGTTGAGGAGGTGGAAGGCG+CGG | 0.558995 | 8.4:-48653086 | None:intergenic |
| AATCAATGTCTTTCCATGCT+CGG | 0.562083 | 8.4:+48653183 | MS.gene38996:CDS |
| ACTTAAAGCGGTAGGGATGA+GGG | 0.569326 | 8.4:-48663869 | None:intergenic |
| TCTTTCCATGCTCGGTGAGG+AGG | 0.570350 | 8.4:+48653191 | MS.gene38996:CDS |
| GGTAGCGGAAGCGGAGGTCA+CGG | 0.570641 | 8.4:-48653125 | None:intergenic |
| CACGCGCAGTCTCTATTCCT+CGG | 0.572306 | 8.4:+48653051 | MS.gene38996:CDS |
| ATGTTTCACTTAAAGCGGTA+GGG | 0.572738 | 8.4:-48663876 | None:intergenic |
| TTTATCCAAGTAGATATTGG+CGG | 0.573210 | 8.4:-48653247 | None:intergenic |
| TCATTTCAGATACCTGTAAG+TGG | 0.574080 | 8.4:-48665057 | None:intergenic |
| TCCCATAGCCTATCCATGCA+GGG | 0.576651 | 8.4:-48661772 | None:intergenic |
| CCTAGTTCGTTATACACAAG+TGG | 0.581962 | 8.4:-48662764 | None:intergenic |
| ACCGTTCTCAGGCTCGATCG+TGG | 0.582145 | 8.4:+48658776 | MS.gene38996:CDS |
| CCAGTATGACAAAGAAATTG+TGG | 0.584615 | 8.4:+48660878 | MS.gene38996:intron |
| TGCATTTGCAGCTAAAGAAG+AGG | 0.589323 | 8.4:+48661796 | MS.gene38996:CDS |
| AATCCATTAGAGTTCAAGGT+GGG | 0.591896 | 8.4:-48666802 | None:intergenic |
| GAGGTTAAAGTCGATGAACA+TGG | 0.593022 | 8.4:+48653210 | MS.gene38996:CDS |
| GCGTGCATATAGATGTCAGC+AGG | 0.594339 | 8.4:-48653033 | None:intergenic |
| ATAGATGTCAGCAGGATCGT+TGG | 0.594352 | 8.4:-48653025 | None:intergenic |
| ACCACGATCGAGCCTGAGAA+CGG | 0.595233 | 8.4:-48658777 | None:intergenic |
| GCTGCAATATGTTTCTTGAG+AGG | 0.595651 | 8.4:+48658730 | MS.gene38996:CDS |
| GATGGCATGCAATTTAGTGA+AGG | 0.598718 | 8.4:+48661289 | MS.gene38996:CDS |
| AATGTGCTCTTGATATGGAA+AGG | 0.600025 | 8.4:-48661215 | None:intergenic |
| CAGCGAGGTAGCGGAAGCGG+AGG | 0.620057 | 8.4:-48653131 | None:intergenic |
| GCCTCCAGGAATTGAACGAG+TGG | 0.620608 | 8.4:+48653160 | MS.gene38996:CDS |
| AATTTGGAAGTGAGGATGGA+TGG | 0.621297 | 8.4:+48660741 | MS.gene38996:CDS |
| GGAAGAAAGAAGGATTAGAC+TGG | 0.629298 | 8.4:-48660947 | None:intergenic |
| TACCGCTTTAAGTGAAACAT+GGG | 0.632859 | 8.4:+48663879 | MS.gene38996:CDS |
| CCATAGCCTATCCATGCAGG+GGG | 0.632890 | 8.4:-48661770 | None:intergenic |
| CCCATAGCCTATCCATGCAG+GGG | 0.636273 | 8.4:-48661771 | None:intergenic |
| ATCTACTTGGATAAAGATCG+TGG | 0.636532 | 8.4:+48653255 | MS.gene38996:CDS |
| GCATTTGCAGCTAAAGAAGA+GGG | 0.638014 | 8.4:+48661797 | MS.gene38996:CDS |
| ATCCATTAGAGTTCAAGGTG+GGG | 0.651866 | 8.4:-48666801 | None:intergenic |
| GTACAAAGCGGCTATAAAAG+CGG | 0.663295 | 8.4:+48659010 | MS.gene38996:CDS |
| GGTATAAGAAAGCAGTGTGG+TGG | 0.663981 | 8.4:+48663830 | MS.gene38996:intron |
| ATAGATGAAAGTAGAAGCAG+CGG | 0.669594 | 8.4:+48666774 | MS.gene38996:CDS |
| ATGTCTTTCCATGCTCGGTG+AGG | 0.672682 | 8.4:+48653188 | MS.gene38996:CDS |
| TCATTGAAAATCAGGCGTTG+TGG | 0.678575 | 8.4:+48666703 | MS.gene38996:intron |
| AAATTTACTTGAAATAACGG+CGG | 0.680832 | 8.4:-48661534 | None:intergenic |
| TCCACTCGTTCAATTCCTGG+AGG | 0.681004 | 8.4:-48653161 | None:intergenic |
| TGAGGAGGTGGAAGGCGCGG+CGG | 0.684256 | 8.4:-48653083 | None:intergenic |
| GCTGAATACTATCATCAGTG+TGG | 0.709549 | 8.4:+48661008 | MS.gene38996:CDS |
| CCTGGAGGCACTGTGCAGCG+AGG | 0.710760 | 8.4:-48653146 | None:intergenic |
| AAATCCATTAGAGTTCAAGG+TGG | 0.717409 | 8.4:-48666803 | None:intergenic |
| AGCGGAAGCGGAGGTCACGG+AGG | 0.720457 | 8.4:-48653122 | None:intergenic |
| GAAATAATCGAGCTGCAGTG+CGG | 0.737569 | 8.4:-48661841 | None:intergenic |
| ATCGGCGAAGAAGATCGCTG+AGG | 0.746804 | 8.4:-48652986 | None:intergenic |
| CATAGCCTATCCATGCAGGG+GGG | 0.751409 | 8.4:-48661769 | None:intergenic |
| TCAGGTATAAGAAAGCAGTG+TGG | 0.802084 | 8.4:+48663827 | MS.gene38996:intron |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr8.4 | gene | 48652967 | 48666818 | 48652967 | ID=MS.gene38996 |
| chr8.4 | mRNA | 48652967 | 48666818 | 48652967 | ID=MS.gene38996.t1;Parent=MS.gene38996 |
| chr8.4 | exon | 48652967 | 48653287 | 48652967 | ID=MS.gene38996.t1.exon1;Parent=MS.gene38996.t1 |
| chr8.4 | CDS | 48652967 | 48653287 | 48652967 | ID=cds.MS.gene38996.t1;Parent=MS.gene38996.t1 |
| chr8.4 | exon | 48658724 | 48658797 | 48658724 | ID=MS.gene38996.t1.exon2;Parent=MS.gene38996.t1 |
| chr8.4 | CDS | 48658724 | 48658797 | 48658724 | ID=cds.MS.gene38996.t1;Parent=MS.gene38996.t1 |
| chr8.4 | exon | 48659010 | 48659049 | 48659010 | ID=MS.gene38996.t1.exon3;Parent=MS.gene38996.t1 |
| chr8.4 | CDS | 48659010 | 48659049 | 48659010 | ID=cds.MS.gene38996.t1;Parent=MS.gene38996.t1 |
| chr8.4 | exon | 48660533 | 48660578 | 48660533 | ID=MS.gene38996.t1.exon4;Parent=MS.gene38996.t1 |
| chr8.4 | CDS | 48660533 | 48660578 | 48660533 | ID=cds.MS.gene38996.t1;Parent=MS.gene38996.t1 |
| chr8.4 | exon | 48660717 | 48660796 | 48660717 | ID=MS.gene38996.t1.exon5;Parent=MS.gene38996.t1 |
| chr8.4 | CDS | 48660717 | 48660796 | 48660717 | ID=cds.MS.gene38996.t1;Parent=MS.gene38996.t1 |
| chr8.4 | exon | 48660882 | 48661063 | 48660882 | ID=MS.gene38996.t1.exon6;Parent=MS.gene38996.t1 |
| chr8.4 | CDS | 48660882 | 48661063 | 48660882 | ID=cds.MS.gene38996.t1;Parent=MS.gene38996.t1 |
| chr8.4 | exon | 48661199 | 48661324 | 48661199 | ID=MS.gene38996.t1.exon7;Parent=MS.gene38996.t1 |
| chr8.4 | CDS | 48661199 | 48661324 | 48661199 | ID=cds.MS.gene38996.t1;Parent=MS.gene38996.t1 |
| chr8.4 | exon | 48661474 | 48661548 | 48661474 | ID=MS.gene38996.t1.exon8;Parent=MS.gene38996.t1 |
| chr8.4 | CDS | 48661474 | 48661548 | 48661474 | ID=cds.MS.gene38996.t1;Parent=MS.gene38996.t1 |
| chr8.4 | exon | 48661742 | 48661867 | 48661742 | ID=MS.gene38996.t1.exon9;Parent=MS.gene38996.t1 |
| chr8.4 | CDS | 48661742 | 48661867 | 48661742 | ID=cds.MS.gene38996.t1;Parent=MS.gene38996.t1 |
| chr8.4 | exon | 48662403 | 48662472 | 48662403 | ID=MS.gene38996.t1.exon10;Parent=MS.gene38996.t1 |
| chr8.4 | CDS | 48662403 | 48662472 | 48662403 | ID=cds.MS.gene38996.t1;Parent=MS.gene38996.t1 |
| chr8.4 | exon | 48662728 | 48662810 | 48662728 | ID=MS.gene38996.t1.exon11;Parent=MS.gene38996.t1 |
| chr8.4 | CDS | 48662728 | 48662810 | 48662728 | ID=cds.MS.gene38996.t1;Parent=MS.gene38996.t1 |
| chr8.4 | exon | 48663831 | 48663944 | 48663831 | ID=MS.gene38996.t1.exon12;Parent=MS.gene38996.t1 |
| chr8.4 | CDS | 48663831 | 48663944 | 48663831 | ID=cds.MS.gene38996.t1;Parent=MS.gene38996.t1 |
| chr8.4 | exon | 48664970 | 48665066 | 48664970 | ID=MS.gene38996.t1.exon13;Parent=MS.gene38996.t1 |
| chr8.4 | CDS | 48664970 | 48665066 | 48664970 | ID=cds.MS.gene38996.t1;Parent=MS.gene38996.t1 |
| chr8.4 | exon | 48666000 | 48666035 | 48666000 | ID=MS.gene38996.t1.exon14;Parent=MS.gene38996.t1 |
| chr8.4 | CDS | 48666000 | 48666035 | 48666000 | ID=cds.MS.gene38996.t1;Parent=MS.gene38996.t1 |
| chr8.4 | exon | 48666717 | 48666818 | 48666717 | ID=MS.gene38996.t1.exon15;Parent=MS.gene38996.t1 |
| chr8.4 | CDS | 48666717 | 48666818 | 48666717 | ID=cds.MS.gene38996.t1;Parent=MS.gene38996.t1 |
| Gene Sequence |
| Protein sequence |
