Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene46055.t1 | XP_003623195.1 | 95.5 | 224 | 10 | 0 | 1 | 224 | 1 | 224 | 1.60E-119 | 438.7 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene46055.t1 | P32110 | 73.8 | 225 | 58 | 1 | 1 | 224 | 1 | 225 | 1.7e-90 | 333.6 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene46055.t1 | G7L6G9 | 95.5 | 224 | 10 | 0 | 1 | 224 | 1 | 224 | 1.1e-119 | 438.7 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049181 | MS.gene46055 | 0.806984 | 6.31E-50 | -1.69E-46 |
| MS.gene049283 | MS.gene46055 | 0.831143 | 1.97E-55 | -1.69E-46 |
| MS.gene049680 | MS.gene46055 | 0.823417 | 1.40E-53 | -1.69E-46 |
| MS.gene050379 | MS.gene46055 | 0.82171 | 3.49E-53 | -1.69E-46 |
| MS.gene050945 | MS.gene46055 | 0.832304 | 1.02E-55 | -1.69E-46 |
| MS.gene05156 | MS.gene46055 | 0.810321 | 1.22E-50 | -1.69E-46 |
| MS.gene051699 | MS.gene46055 | 0.820049 | 8.42E-53 | -1.69E-46 |
| MS.gene051959 | MS.gene46055 | 0.831763 | 1.39E-55 | -1.69E-46 |
| MS.gene051960 | MS.gene46055 | 0.831106 | 2.01E-55 | -1.69E-46 |
| MS.gene052040 | MS.gene46055 | 0.813421 | 2.58E-51 | -1.69E-46 |
| MS.gene052053 | MS.gene46055 | 0.851233 | 1.01E-60 | -1.69E-46 |
| MS.gene052533 | MS.gene46055 | 0.80797 | 3.90E-50 | -1.69E-46 |
| MS.gene052559 | MS.gene46055 | 0.806239 | 9.06E-50 | -1.69E-46 |
| MS.gene053602 | MS.gene46055 | 0.820093 | 8.23E-53 | -1.69E-46 |
| MS.gene053963 | MS.gene46055 | 0.809628 | 1.72E-50 | -1.69E-46 |
| MS.gene054413 | MS.gene46055 | 0.829617 | 4.66E-55 | -1.69E-46 |
| MS.gene054892 | MS.gene46055 | 0.801689 | 8.00E-49 | -1.69E-46 |
| MS.gene054893 | MS.gene46055 | 0.808321 | 3.28E-50 | -1.69E-46 |
| MS.gene054957 | MS.gene46055 | 0.850274 | 1.89E-60 | -1.69E-46 |
| MS.gene054960 | MS.gene46055 | 0.801949 | 7.07E-49 | -1.69E-46 |
| MS.gene055147 | MS.gene46055 | 0.810711 | 1.01E-50 | -1.69E-46 |
| MS.gene055148 | MS.gene46055 | 0.814669 | 1.37E-51 | -1.69E-46 |
| MS.gene055150 | MS.gene46055 | 0.925896 | 9.85E-91 | -1.69E-46 |
| MS.gene055151 | MS.gene46055 | 0.810968 | 8.85E-51 | -1.69E-46 |
| MS.gene055155 | MS.gene46055 | 0.815234 | 1.03E-51 | -1.69E-46 |
| MS.gene05624 | MS.gene46055 | 0.830544 | 2.77E-55 | -1.69E-46 |
| MS.gene056530 | MS.gene46055 | 0.860426 | 2.08E-63 | -1.69E-46 |
| MS.gene05667 | MS.gene46055 | 0.825362 | 4.88E-54 | -1.69E-46 |
| MS.gene057102 | MS.gene46055 | 0.805223 | 1.48E-49 | -1.69E-46 |
| MS.gene057148 | MS.gene46055 | -0.822029 | 2.95E-53 | -1.69E-46 |
| MS.gene057472 | MS.gene46055 | 0.85665 | 2.79E-62 | -1.69E-46 |
| MS.gene057622 | MS.gene46055 | 0.832383 | 9.76E-56 | -1.69E-46 |
| MS.gene058592 | MS.gene46055 | 0.834012 | 3.83E-56 | -1.69E-46 |
| MS.gene05878 | MS.gene46055 | 0.862761 | 4.03E-64 | -1.69E-46 |
| MS.gene059436 | MS.gene46055 | 0.846179 | 2.56E-59 | -1.69E-46 |
| MS.gene05952 | MS.gene46055 | 0.870426 | 1.47E-66 | -1.69E-46 |
| MS.gene05956 | MS.gene46055 | 0.808648 | 2.79E-50 | -1.69E-46 |
| MS.gene05985 | MS.gene46055 | -0.85324 | 2.72E-61 | -1.69E-46 |
| MS.gene059901 | MS.gene46055 | -0.815505 | 8.93E-52 | -1.69E-46 |
| MS.gene060237 | MS.gene46055 | 0.808817 | 2.57E-50 | -1.69E-46 |
| MS.gene06023 | MS.gene46055 | 0.836557 | 8.73E-57 | -1.69E-46 |
| MS.gene060338 | MS.gene46055 | 0.815274 | 1.01E-51 | -1.69E-46 |
| MS.gene060935 | MS.gene46055 | 0.800541 | 1.37E-48 | -1.69E-46 |
| MS.gene060937 | MS.gene46055 | 0.818481 | 1.92E-52 | -1.69E-46 |
| MS.gene060975 | MS.gene46055 | 0.82996 | 3.84E-55 | -1.69E-46 |
| MS.gene060977 | MS.gene46055 | 0.857252 | 1.85E-62 | -1.69E-46 |
| MS.gene061243 | MS.gene46055 | 0.81649 | 5.38E-52 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene46055.t1 | MTR_7g065590 | 95.536 | 224 | 10 | 0 | 1 | 224 | 1 | 224 | 3.56e-158 | 437 |
| MS.gene46055.t1 | MTR_7g065600 | 80.804 | 224 | 43 | 0 | 1 | 224 | 1 | 224 | 2.25e-134 | 377 |
| MS.gene46055.t1 | MTR_7g065290 | 80.804 | 224 | 43 | 0 | 1 | 224 | 1 | 224 | 4.64e-130 | 365 |
| MS.gene46055.t1 | MTR_7g065230 | 79.018 | 224 | 42 | 1 | 1 | 224 | 1 | 219 | 1.02e-124 | 352 |
| MS.gene46055.t1 | MTR_7g065265 | 78.125 | 224 | 49 | 0 | 1 | 224 | 1 | 224 | 2.50e-124 | 351 |
| MS.gene46055.t1 | MTR_7g065270 | 76.786 | 224 | 52 | 0 | 1 | 224 | 1 | 224 | 7.47e-124 | 350 |
| MS.gene46055.t1 | MTR_7g065260 | 79.111 | 225 | 46 | 1 | 1 | 224 | 8 | 232 | 9.05e-117 | 332 |
| MS.gene46055.t1 | MTR_7g065660 | 68.610 | 223 | 70 | 0 | 1 | 223 | 1 | 223 | 5.13e-114 | 325 |
| MS.gene46055.t1 | MTR_7g065720 | 69.333 | 225 | 68 | 1 | 1 | 224 | 1 | 225 | 1.65e-113 | 324 |
| MS.gene46055.t1 | MTR_7g065630 | 69.955 | 223 | 63 | 1 | 1 | 223 | 19 | 237 | 4.97e-113 | 323 |
| MS.gene46055.t1 | MTR_7g065740 | 64.732 | 224 | 78 | 1 | 1 | 223 | 1 | 224 | 1.15e-108 | 311 |
| MS.gene46055.t1 | MTR_7g065750 | 66.518 | 224 | 74 | 1 | 1 | 223 | 1 | 224 | 1.27e-108 | 311 |
| MS.gene46055.t1 | MTR_4g019790 | 63.229 | 223 | 82 | 0 | 1 | 223 | 1 | 223 | 1.88e-105 | 303 |
| MS.gene46055.t1 | MTR_7g065640 | 64.126 | 223 | 62 | 1 | 1 | 223 | 1 | 205 | 1.48e-101 | 293 |
| MS.gene46055.t1 | MTR_7g065680 | 67.117 | 222 | 69 | 1 | 1 | 222 | 1 | 218 | 9.10e-98 | 284 |
| MS.gene46055.t1 | MTR_7g065710 | 66.184 | 207 | 66 | 1 | 8 | 214 | 5 | 207 | 6.05e-97 | 282 |
| MS.gene46055.t1 | MTR_4g019780 | 63.256 | 215 | 77 | 2 | 2 | 215 | 3 | 216 | 2.17e-96 | 280 |
| MS.gene46055.t1 | MTR_7g065700 | 66.822 | 214 | 67 | 1 | 1 | 214 | 1 | 210 | 4.01e-93 | 272 |
| MS.gene46055.t1 | MTR_6g080440 | 48.077 | 208 | 106 | 2 | 7 | 213 | 3 | 209 | 1.06e-65 | 202 |
| MS.gene46055.t1 | MTR_3g099757 | 47.727 | 220 | 113 | 2 | 5 | 223 | 3 | 221 | 3.81e-65 | 201 |
| MS.gene46055.t1 | MTR_1g090150 | 47.059 | 221 | 112 | 3 | 5 | 222 | 1 | 219 | 2.29e-64 | 199 |
| MS.gene46055.t1 | MTR_8g087410 | 47.926 | 217 | 110 | 3 | 1 | 216 | 1 | 215 | 4.01e-63 | 196 |
| MS.gene46055.t1 | MTR_8g087425 | 45.622 | 217 | 116 | 2 | 4 | 219 | 2 | 217 | 1.77e-61 | 192 |
| MS.gene46055.t1 | MTR_1g115195 | 45.000 | 220 | 114 | 3 | 7 | 221 | 3 | 220 | 3.05e-60 | 189 |
| MS.gene46055.t1 | MTR_5g040430 | 42.793 | 222 | 119 | 3 | 1 | 215 | 1 | 221 | 1.23e-56 | 179 |
| MS.gene46055.t1 | MTR_1g090070 | 42.534 | 221 | 122 | 3 | 7 | 223 | 9 | 228 | 1.29e-56 | 179 |
| MS.gene46055.t1 | MTR_4g059730 | 42.056 | 214 | 118 | 3 | 6 | 215 | 3 | 214 | 6.68e-56 | 177 |
| MS.gene46055.t1 | MTR_1g090060 | 42.857 | 224 | 118 | 5 | 7 | 224 | 3 | 222 | 1.36e-55 | 177 |
| MS.gene46055.t1 | MTR_1g090100 | 41.818 | 220 | 122 | 3 | 8 | 223 | 3 | 220 | 2.07e-53 | 171 |
| MS.gene46055.t1 | MTR_1g090090 | 42.381 | 210 | 115 | 3 | 7 | 212 | 3 | 210 | 4.00e-52 | 168 |
| MS.gene46055.t1 | MTR_8g061950 | 43.836 | 219 | 115 | 5 | 5 | 215 | 3 | 221 | 6.08e-50 | 162 |
| MS.gene46055.t1 | MTR_5g037380 | 42.273 | 220 | 117 | 4 | 1 | 212 | 1 | 218 | 5.82e-49 | 160 |
| MS.gene46055.t1 | MTR_2g070150 | 38.235 | 204 | 121 | 4 | 6 | 207 | 3 | 203 | 5.45e-43 | 144 |
| MS.gene46055.t1 | MTR_3g467420 | 42.211 | 199 | 110 | 5 | 16 | 212 | 18 | 213 | 2.91e-42 | 143 |
| MS.gene46055.t1 | MTR_2g070130 | 38.350 | 206 | 122 | 4 | 6 | 209 | 3 | 205 | 3.95e-42 | 142 |
| MS.gene46055.t1 | MTR_2g070200 | 38.863 | 211 | 124 | 4 | 6 | 213 | 3 | 211 | 3.95e-42 | 142 |
| MS.gene46055.t1 | MTR_4g124130 | 45.263 | 190 | 98 | 5 | 5 | 191 | 2 | 188 | 1.56e-41 | 140 |
| MS.gene46055.t1 | MTR_2g070140 | 37.949 | 195 | 116 | 4 | 21 | 213 | 2 | 193 | 6.08e-41 | 139 |
| MS.gene46055.t1 | MTR_2g070120 | 39.906 | 213 | 117 | 7 | 6 | 213 | 3 | 209 | 2.09e-40 | 138 |
| MS.gene46055.t1 | MTR_2g070180 | 36.765 | 204 | 124 | 4 | 6 | 207 | 3 | 203 | 4.24e-40 | 137 |
| MS.gene46055.t1 | MTR_2g070210 | 38.679 | 212 | 123 | 5 | 6 | 213 | 3 | 211 | 6.00e-40 | 137 |
| MS.gene46055.t1 | MTR_3g467430 | 38.863 | 211 | 124 | 5 | 4 | 212 | 6 | 213 | 6.51e-40 | 137 |
| MS.gene46055.t1 | MTR_8g056940 | 37.900 | 219 | 126 | 6 | 6 | 218 | 3 | 217 | 2.51e-38 | 132 |
| MS.gene46055.t1 | MTR_2g070060 | 37.500 | 208 | 125 | 4 | 7 | 212 | 4 | 208 | 1.37e-37 | 130 |
| MS.gene46055.t1 | MTR_2g070070 | 37.500 | 216 | 130 | 4 | 7 | 220 | 4 | 216 | 3.04e-37 | 130 |
| MS.gene46055.t1 | MTR_5g076900 | 36.321 | 212 | 130 | 4 | 3 | 212 | 2 | 210 | 1.26e-35 | 125 |
| MS.gene46055.t1 | MTR_2g070110 | 39.500 | 200 | 115 | 5 | 7 | 204 | 4 | 199 | 2.39e-32 | 117 |
| MS.gene46055.t1 | MTR_0186s0030 | 53.191 | 94 | 43 | 1 | 16 | 108 | 13 | 106 | 4.32e-31 | 111 |
| MS.gene46055.t1 | MTR_2g072120 | 45.714 | 70 | 36 | 2 | 51 | 119 | 2 | 70 | 5.04e-16 | 70.5 |
| MS.gene46055.t1 | MTR_4g134370 | 30.000 | 230 | 129 | 9 | 1 | 207 | 1 | 221 | 1.43e-12 | 65.1 |
| MS.gene46055.t1 | MTR_1g067180 | 26.667 | 225 | 131 | 8 | 8 | 217 | 25 | 230 | 7.40e-12 | 63.2 |
| MS.gene46055.t1 | MTR_1g067180 | 26.667 | 225 | 131 | 8 | 8 | 217 | 28 | 233 | 1.88e-11 | 62.0 |
| MS.gene46055.t1 | MTR_5g037390 | 36.957 | 92 | 46 | 2 | 31 | 119 | 11 | 93 | 7.96e-11 | 57.4 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene46055.t1 | AT3G09270 | 54.018 | 224 | 98 | 5 | 4 | 223 | 2 | 224 | 4.31e-76 | 229 |
| MS.gene46055.t1 | AT2G29420 | 49.315 | 219 | 109 | 2 | 3 | 220 | 5 | 222 | 9.33e-75 | 226 |
| MS.gene46055.t1 | AT2G29450 | 49.275 | 207 | 103 | 2 | 3 | 207 | 2 | 208 | 1.71e-60 | 189 |
| MS.gene46055.t1 | AT2G29490 | 48.325 | 209 | 105 | 3 | 1 | 207 | 1 | 208 | 3.58e-60 | 189 |
| MS.gene46055.t1 | AT2G29480 | 47.368 | 209 | 107 | 3 | 1 | 207 | 1 | 208 | 4.81e-58 | 183 |
| MS.gene46055.t1 | AT2G29460 | 45.333 | 225 | 120 | 3 | 1 | 223 | 1 | 224 | 1.51e-57 | 182 |
| MS.gene46055.t1 | AT2G29470 | 46.190 | 210 | 109 | 4 | 1 | 207 | 1 | 209 | 1.64e-56 | 179 |
| MS.gene46055.t1 | AT2G29440 | 46.411 | 209 | 108 | 4 | 1 | 207 | 1 | 207 | 1.33e-55 | 177 |
| MS.gene46055.t1 | AT1G69930 | 38.938 | 226 | 132 | 3 | 2 | 221 | 7 | 232 | 9.37e-50 | 162 |
| MS.gene46055.t1 | AT1G59700 | 44.037 | 218 | 114 | 4 | 5 | 214 | 4 | 221 | 1.53e-48 | 159 |
| MS.gene46055.t1 | AT1G10370 | 40.724 | 221 | 122 | 4 | 1 | 214 | 1 | 219 | 2.56e-47 | 156 |
| MS.gene46055.t1 | AT1G59670 | 42.202 | 218 | 118 | 4 | 5 | 214 | 4 | 221 | 3.17e-47 | 155 |
| MS.gene46055.t1 | AT1G74590 | 39.545 | 220 | 126 | 3 | 1 | 214 | 1 | 219 | 4.65e-47 | 155 |
| MS.gene46055.t1 | AT1G78320 | 40.865 | 208 | 118 | 4 | 5 | 210 | 2 | 206 | 5.66e-47 | 155 |
| MS.gene46055.t1 | AT5G62480 | 39.819 | 221 | 122 | 4 | 5 | 215 | 6 | 225 | 1.97e-46 | 154 |
| MS.gene46055.t1 | AT1G78340 | 42.105 | 209 | 116 | 4 | 6 | 212 | 3 | 208 | 1.04e-45 | 151 |
| MS.gene46055.t1 | AT1G27130 | 36.889 | 225 | 133 | 4 | 1 | 217 | 1 | 224 | 2.79e-45 | 150 |
| MS.gene46055.t1 | AT1G78370 | 40.201 | 199 | 114 | 4 | 16 | 212 | 13 | 208 | 1.02e-43 | 146 |
| MS.gene46055.t1 | AT1G10360 | 37.037 | 216 | 129 | 3 | 6 | 214 | 4 | 219 | 1.35e-43 | 146 |
| MS.gene46055.t1 | AT1G78380 | 39.904 | 208 | 120 | 4 | 7 | 212 | 4 | 208 | 1.46e-43 | 146 |
| MS.gene46055.t1 | AT1G17170 | 38.710 | 217 | 127 | 5 | 6 | 220 | 3 | 215 | 2.00e-43 | 145 |
| MS.gene46055.t1 | AT1G27140 | 36.564 | 227 | 134 | 4 | 1 | 218 | 1 | 226 | 4.08e-43 | 145 |
| MS.gene46055.t1 | AT1G69920 | 37.118 | 229 | 130 | 5 | 1 | 217 | 26 | 252 | 2.29e-42 | 144 |
| MS.gene46055.t1 | AT1G17190 | 42.487 | 193 | 104 | 5 | 1 | 191 | 1 | 188 | 2.99e-41 | 140 |
| MS.gene46055.t1 | AT1G78360 | 40.191 | 209 | 119 | 5 | 7 | 212 | 4 | 209 | 8.01e-40 | 136 |
| MS.gene46055.t1 | AT1G17180 | 37.799 | 209 | 125 | 4 | 6 | 212 | 3 | 208 | 1.75e-39 | 135 |
| MS.gene46055.t1 | AT3G43800 | 37.198 | 207 | 121 | 6 | 4 | 204 | 2 | 205 | 1.54e-38 | 133 |
| MS.gene46055.t1 | AT1G53680 | 36.667 | 210 | 128 | 4 | 1 | 207 | 1 | 208 | 9.61e-38 | 131 |
| MS.gene46055.t1 | AT1G78320 | 32.850 | 207 | 109 | 4 | 5 | 210 | 2 | 179 | 9.55e-29 | 107 |
| MS.gene46055.t1 | AT5G62480 | 31.364 | 220 | 115 | 4 | 5 | 215 | 6 | 198 | 1.03e-27 | 105 |
| MS.gene46055.t1 | AT5G62480 | 31.364 | 220 | 116 | 4 | 5 | 215 | 6 | 199 | 1.11e-27 | 105 |
| MS.gene46055.t1 | AT2G02380 | 28.704 | 216 | 133 | 7 | 5 | 207 | 9 | 216 | 3.54e-14 | 69.3 |
| MS.gene46055.t1 | AT2G02390 | 28.444 | 225 | 133 | 7 | 3 | 207 | 4 | 220 | 5.48e-13 | 66.2 |
| MS.gene46055.t1 | AT2G02390 | 29.358 | 218 | 133 | 7 | 3 | 207 | 4 | 213 | 7.76e-13 | 65.9 |
Find 38 sgRNAs with CRISPR-Local
Find 65 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AAAGAGCCTTGGCTCGTTTC+TGG | 0.160272 | 7.2:-42856480 | MS.gene46055:CDS |
| ATTTCAACCCTTGTAGAAAA+TGG | 0.235130 | 7.2:+42856709 | None:intergenic |
| ACAGGAACTTTCTTATAAAC+AGG | 0.283283 | 7.2:+42856604 | None:intergenic |
| CCTGTTACTTCTTGTACAAT+AGG | 0.286054 | 7.2:+42855959 | None:intergenic |
| TTGGGTGGTTGTTGAATTCT+TGG | 0.290435 | 7.2:+42855888 | None:intergenic |
| GAGCTCAAGGGCAAGTTCTT+TGG | 0.302605 | 7.2:-42856037 | MS.gene46055:CDS |
| GCCTCGTAGAGAAGGGCTTT+TGG | 0.332731 | 7.2:-42855849 | MS.gene46055:CDS |
| TAAGGATCAGAAGGCAAGAT+TGG | 0.379369 | 7.2:+42856505 | None:intergenic |
| GTTCCTGTATTAGTTCATAA+TGG | 0.400419 | 7.2:-42856589 | MS.gene46055:CDS |
| CACTAAGATGGCATCAAATC+AGG | 0.403482 | 7.2:-42856764 | None:intergenic |
| TTCTTTGGTGGAGATGAGAT+TGG | 0.406091 | 7.2:-42856022 | MS.gene46055:CDS |
| CCTATTGTACAAGAAGTAAC+AGG | 0.429315 | 7.2:-42855959 | MS.gene46055:CDS |
| GTCCAAGTTCATAGATGATA+AGG | 0.434027 | 7.2:-42856458 | MS.gene46055:intron |
| CTGTTACTTCTTGTACAATA+GGG | 0.437463 | 7.2:+42855960 | None:intergenic |
| TGGCTCCAATTGTAAAGCTT+AGG | 0.444725 | 7.2:+42855908 | None:intergenic |
| AATTTCCTAAGCTTTACAAT+TGG | 0.445365 | 7.2:-42855913 | MS.gene46055:CDS |
| TGAAATAGCTCTAAAATTGA+AGG | 0.446842 | 7.2:-42856692 | MS.gene46055:CDS |
| CAAATATGAGGAAGAAAAGC+AGG | 0.457444 | 7.2:-42856659 | MS.gene46055:CDS |
| TACCTTATCATCTATGAACT+TGG | 0.459673 | 7.2:+42856456 | None:intergenic |
| GAAAATTTGCCTCGTAGAGA+AGG | 0.475442 | 7.2:-42855857 | MS.gene46055:CDS |
| TTGCCATTATGAACTAATAC+AGG | 0.495358 | 7.2:+42856586 | None:intergenic |
| ATCACAAGTGACTCTGCTAT+AGG | 0.496284 | 7.2:+42856562 | None:intergenic |
| CTCAAGGGCAAGTTCTTTGG+TGG | 0.497594 | 7.2:-42856034 | MS.gene46055:CDS |
| TTGAATATATTGATGAGACT+TGG | 0.500568 | 7.2:-42856537 | MS.gene46055:CDS |
| TTCATAACGAGCTTTGAAGA+AGG | 0.517849 | 7.2:+42855826 | None:intergenic |
| GGGTGTTGAATACAAATATG+AGG | 0.518356 | 7.2:-42856671 | MS.gene46055:CDS |
| TGATCCTTATAAAAGAGCCT+TGG | 0.539754 | 7.2:-42856491 | MS.gene46055:CDS |
| AAAATTTGCCTCGTAGAGAA+GGG | 0.551755 | 7.2:-42855856 | MS.gene46055:CDS |
| ATTTCTTGAGAATGAGCTCA+AGG | 0.554660 | 7.2:-42856050 | MS.gene46055:CDS |
| CAGGAACTTTCTTATAAACA+GGG | 0.564477 | 7.2:+42856605 | None:intergenic |
| GAAATAGCTCTAAAATTGAA+GGG | 0.600047 | 7.2:-42856691 | MS.gene46055:CDS |
| CAACAACCACCCAATTGTCA+AGG | 0.606941 | 7.2:-42855879 | MS.gene46055:CDS |
| TTTCTTGAGAATGAGCTCAA+GGG | 0.611348 | 7.2:-42856049 | MS.gene46055:CDS |
| AAATATGAGGAAGAAAAGCA+GGG | 0.615123 | 7.2:-42856658 | MS.gene46055:CDS |
| GCCAAAAGCCCTTCTCTACG+AGG | 0.649932 | 7.2:+42855848 | None:intergenic |
| ACCAGTGTATGATTCCTGCA+TGG | 0.670185 | 7.2:-42856141 | MS.gene46055:intron |
| CTTGGACCAGAAACGAGCCA+AGG | 0.694386 | 7.2:+42856474 | None:intergenic |
| AATATGAGGAAGAAAAGCAG+GGG | 0.696106 | 7.2:-42856657 | MS.gene46055:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | ATATATATATATAATGAACT+TGG | + | chr7.2:42856168-42856187 | None:intergenic | 10.0% |
| !!! | CTTTTTTCTTTTTTTTTTAA+TGG | + | chr7.2:42856235-42856254 | None:intergenic | 10.0% |
| !! | TAATCATATTTGAGAAATAT+TGG | + | chr7.2:42856308-42856327 | None:intergenic | 15.0% |
| !! | TTAAGGTCATTATAAAAATT+AGG | + | chr7.2:42856393-42856412 | None:intergenic | 15.0% |
| !!! | TTGAATATTGATTAGAAAAA+GGG | - | chr7.2:42856281-42856300 | MS.gene46055:intron | 15.0% |
| !!! | TTTGAATATTGATTAGAAAA+AGG | - | chr7.2:42856280-42856299 | MS.gene46055:intron | 15.0% |
| ! | AATTTCCTAAGCTTTACAAT+TGG | - | chr7.2:42856649-42856668 | MS.gene46055:CDS | 25.0% |
| ! | CAAATATGATTAAGTTAGCT+GGG | - | chr7.2:42856316-42856335 | MS.gene46055:intron | 25.0% |
| ! | TCAAATATGATTAAGTTAGC+TGG | - | chr7.2:42856315-42856334 | MS.gene46055:intron | 25.0% |
| ! | TTGAATATATTGATGAGACT+TGG | - | chr7.2:42856025-42856044 | MS.gene46055:CDS | 25.0% |
| !!! | GAAATAGCTCTAAAATTGAA+GGG | - | chr7.2:42855871-42855890 | MS.gene46055:CDS | 25.0% |
| !!! | TGAAATAGCTCTAAAATTGA+AGG | - | chr7.2:42855870-42855889 | MS.gene46055:CDS | 25.0% |
| AAATATGAGGAAGAAAAGCA+GGG | - | chr7.2:42855904-42855923 | MS.gene46055:CDS | 30.0% | |
| ACAGGAACTTTCTTATAAAC+AGG | + | chr7.2:42855961-42855980 | None:intergenic | 30.0% | |
| ATTTCAACCCTTGTAGAAAA+TGG | + | chr7.2:42855856-42855875 | None:intergenic | 30.0% | |
| CAGGAACTTTCTTATAAACA+GGG | + | chr7.2:42855960-42855979 | None:intergenic | 30.0% | |
| GTTCCTGTATTAGTTCATAA+TGG | - | chr7.2:42855973-42855992 | MS.gene46055:CDS | 30.0% | |
| TACCTTATCATCTATGAACT+TGG | + | chr7.2:42856109-42856128 | None:intergenic | 30.0% | |
| TTGCCATTATGAACTAATAC+AGG | + | chr7.2:42855979-42855998 | None:intergenic | 30.0% | |
| ! | ATATAATGAACTTGGACTAG+AGG | + | chr7.2:42856160-42856179 | None:intergenic | 30.0% |
| ! | CTGTTACTTCTTGTACAATA+GGG | + | chr7.2:42856605-42856624 | None:intergenic | 30.0% |
| ! | GCAAATTTTCCTTGACAATT+GGG | + | chr7.2:42856695-42856714 | None:intergenic | 30.0% |
| AAAATTTGCCTCGTAGAGAA+GGG | - | chr7.2:42856706-42856725 | MS.gene46055:CDS | 35.0% | |
| AAGAAAAGGGATCAGAAGAT+TGG | + | chr7.2:42856135-42856154 | None:intergenic | 35.0% | |
| AATATGAGGAAGAAAAGCAG+GGG | - | chr7.2:42855905-42855924 | MS.gene46055:CDS | 35.0% | |
| ATTTCTTGAGAATGAGCTCA+AGG | - | chr7.2:42856512-42856531 | MS.gene46055:CDS | 35.0% | |
| CAAATATGAGGAAGAAAAGC+AGG | - | chr7.2:42855903-42855922 | MS.gene46055:CDS | 35.0% | |
| CCTATTGTACAAGAAGTAAC+AGG | - | chr7.2:42856603-42856622 | MS.gene46055:CDS | 35.0% | |
| GTCCAAGTTCATAGATGATA+AGG | - | chr7.2:42856104-42856123 | MS.gene46055:CDS | 35.0% | |
| TGATCCTTATAAAAGAGCCT+TGG | - | chr7.2:42856071-42856090 | MS.gene46055:CDS | 35.0% | |
| TTTCTTGAGAATGAGCTCAA+GGG | - | chr7.2:42856513-42856532 | MS.gene46055:CDS | 35.0% | |
| ! | AATTTTCCTTGACAATTGGG+TGG | + | chr7.2:42856692-42856711 | None:intergenic | 35.0% |
| ! | CCTGTTACTTCTTGTACAAT+AGG | + | chr7.2:42856606-42856625 | None:intergenic | 35.0% |
| ! | CTGCTGTCTTTATAGCTTTT+TGG | - | chr7.2:42856577-42856596 | MS.gene46055:CDS | 35.0% |
| ! | GCTCTTTTATAAGGATCAGA+AGG | + | chr7.2:42856069-42856088 | None:intergenic | 35.0% |
| ! | GGCAAATTTTCCTTGACAAT+TGG | + | chr7.2:42856696-42856715 | None:intergenic | 35.0% |
| ! | TACACTGGTCTCACAAATTA+AGG | + | chr7.2:42856410-42856429 | None:intergenic | 35.0% |
| !! | GGGTGTTGAATACAAATATG+AGG | - | chr7.2:42855891-42855910 | MS.gene46055:CDS | 35.0% |
| !! | TTCATAACGAGCTTTGAAGA+AGG | + | chr7.2:42856739-42856758 | None:intergenic | 35.0% |
| !!! | GAGTTTTGAGGAAACAAAAG+AGG | - | chr7.2:42856482-42856501 | MS.gene46055:CDS | 35.0% |
| ATCACAAGTGACTCTGCTAT+AGG | + | chr7.2:42856003-42856022 | None:intergenic | 40.0% | |
| GAAAATTTGCCTCGTAGAGA+AGG | - | chr7.2:42856705-42856724 | MS.gene46055:CDS | 40.0% | |
| TAAGGATCAGAAGGCAAGAT+TGG | + | chr7.2:42856060-42856079 | None:intergenic | 40.0% | |
| TGGCTCCAATTGTAAAGCTT+AGG | + | chr7.2:42856657-42856676 | None:intergenic | 40.0% | |
| TTCTTTGGTGGAGATGAGAT+TGG | - | chr7.2:42856540-42856559 | MS.gene46055:CDS | 40.0% | |
| TTGGGTGGTTGTTGAATTCT+TGG | + | chr7.2:42856677-42856696 | None:intergenic | 40.0% | |
| ! | AGAACGTGAGAAGAGTTTTG+AGG | - | chr7.2:42856470-42856489 | MS.gene46055:CDS | 40.0% |
| ! | CAGGAAGAAGTGAAGCTTTT+CGG | - | chr7.2:42855817-42855836 | MS.gene46055:CDS | 40.0% |
| ! | GGGAAGTCCATTTTCTACAA+GGG | - | chr7.2:42855846-42855865 | MS.gene46055:CDS | 40.0% |
| ! | TGGACTAGAGGTCAAGAAAA+GGG | + | chr7.2:42856148-42856167 | None:intergenic | 40.0% |
| ! | TGGGAAGTCCATTTTCTACA+AGG | - | chr7.2:42855845-42855864 | MS.gene46055:CDS | 40.0% |
| ! | TTGGACTAGAGGTCAAGAAA+AGG | + | chr7.2:42856149-42856168 | None:intergenic | 40.0% |
| ACCAGTGTATGATTCCTGCA+TGG | - | chr7.2:42856421-42856440 | MS.gene46055:intron | 45.0% | |
| CAACAACCACCCAATTGTCA+AGG | - | chr7.2:42856683-42856702 | MS.gene46055:CDS | 45.0% | |
| TCCATGCAGGAATCATACAC+TGG | + | chr7.2:42856425-42856444 | None:intergenic | 45.0% | |
| ! | AGTGAAGCTTTTCGGACTTG+TGG | - | chr7.2:42855825-42855844 | MS.gene46055:CDS | 45.0% |
| ! | CGAGCCAAGGCTCTTTTATA+AGG | + | chr7.2:42856078-42856097 | None:intergenic | 45.0% |
| ! | GTGAAAGCAGTTTTCCATGC+AGG | + | chr7.2:42856438-42856457 | None:intergenic | 45.0% |
| ! | GTGAAGCTTTTCGGACTTGT+GGG | - | chr7.2:42855826-42855845 | MS.gene46055:CDS | 45.0% |
| CTCAAGGGCAAGTTCTTTGG+TGG | - | chr7.2:42856528-42856547 | MS.gene46055:CDS | 50.0% | |
| GAGCTCAAGGGCAAGTTCTT+TGG | - | chr7.2:42856525-42856544 | MS.gene46055:CDS | 50.0% | |
| ! | AAAGAGCCTTGGCTCGTTTC+TGG | - | chr7.2:42856082-42856101 | MS.gene46055:CDS | 50.0% |
| CTTGGACCAGAAACGAGCCA+AGG | + | chr7.2:42856091-42856110 | None:intergenic | 55.0% | |
| GCCAAAAGCCCTTCTCTACG+AGG | + | chr7.2:42856717-42856736 | None:intergenic | 55.0% | |
| ! | GCCTCGTAGAGAAGGGCTTT+TGG | - | chr7.2:42856713-42856732 | MS.gene46055:CDS | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr7.2 | gene | 42855805 | 42856779 | 42855805 | ID=MS.gene46055 |
| chr7.2 | mRNA | 42855805 | 42856779 | 42855805 | ID=MS.gene46055.t1;Parent=MS.gene46055 |
| chr7.2 | exon | 42856459 | 42856779 | 42856459 | ID=MS.gene46055.t1.exon1;Parent=MS.gene46055.t1 |
| chr7.2 | CDS | 42856459 | 42856779 | 42856459 | ID=cds.MS.gene46055.t1;Parent=MS.gene46055.t1 |
| chr7.2 | exon | 42855805 | 42856158 | 42855805 | ID=MS.gene46055.t1.exon2;Parent=MS.gene46055.t1 |
| chr7.2 | CDS | 42855805 | 42856158 | 42855805 | ID=cds.MS.gene46055.t1;Parent=MS.gene46055.t1 |
| Gene Sequence |
| Protein sequence |
