AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene54952

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene54952.t1 MTR_7g083870 98.671 301 3 1 1 300 1 301 0.0 600
MS.gene54952.t1 MTR_7g107670 56.884 276 118 1 23 298 22 296 2.83e-112 327
MS.gene54952.t1 MTR_7g071680 49.265 272 125 4 32 290 27 298 1.58e-82 251
MS.gene54952.t1 MTR_4g052320 49.807 259 121 4 33 290 7 257 3.05e-78 239
MS.gene54952.t1 MTR_4g052350 51.953 256 113 5 32 286 12 258 2.73e-77 237
MS.gene54952.t1 MTR_4g052300 50.187 267 122 5 33 298 7 263 1.48e-76 234
MS.gene54952.t1 MTR_4g052340 50.190 263 120 6 32 293 12 264 9.39e-75 230
MS.gene54952.t1 MTR_3g113870 52.683 205 96 1 42 246 29 232 2.72e-72 223
MS.gene54952.t1 MTR_7g110140 47.566 267 131 4 33 297 5 264 5.50e-70 218
MS.gene54952.t1 MTR_7g110130 45.594 261 134 4 32 290 4 258 9.81e-69 214
MS.gene54952.t1 MTR_7g105460 49.627 268 126 6 27 293 7 266 3.08e-68 214
MS.gene54952.t1 MTR_4g078630 45.956 272 136 7 32 296 17 284 1.15e-67 213
MS.gene54952.t1 MTR_2g090165 45.113 266 136 7 32 295 4 261 2.18e-67 211
MS.gene54952.t1 MTR_3g020670 46.970 264 124 4 32 286 14 270 4.88e-66 208
MS.gene54952.t1 MTR_7g110160 45.038 262 117 6 32 291 4 240 1.14e-62 198
MS.gene54952.t1 MTR_6g035395 45.000 260 123 5 32 290 3 243 1.62e-62 198
MS.gene54952.t1 MTR_6g023590 41.797 256 140 5 33 285 12 261 1.04e-59 191
MS.gene54952.t1 MTR_6g023990 41.016 256 142 5 33 285 13 262 3.42e-59 190
MS.gene54952.t1 MTR_7g105580 39.773 264 134 3 31 293 5 244 1.74e-58 188
MS.gene54952.t1 MTR_6g023610 40.076 262 148 5 27 285 6 261 6.22e-56 182
MS.gene54952.t1 MTR_6g023910 40.310 258 141 6 33 285 12 261 1.15e-55 181
MS.gene54952.t1 MTR_7g105590 49.474 190 94 2 31 219 5 193 2.68e-54 175
MS.gene54952.t1 MTR_6g023950 40.000 260 145 6 33 288 12 264 4.46e-53 174
MS.gene54952.t1 MTR_6g023600 39.453 256 146 5 33 285 12 261 8.92e-52 171
MS.gene54952.t1 MTR_6g023600 38.672 256 142 6 33 285 12 255 3.81e-47 159
MS.gene54952.t1 MTR_7g105570 40.684 263 99 6 31 293 18 223 1.10e-44 152
MS.gene54952.t1 MTR_4g084800 56.489 131 55 2 32 161 11 140 4.33e-42 142
MS.gene54952.t1 MTR_3g085740 31.274 259 160 6 32 285 73 318 1.93e-27 108
MS.gene54952.t1 MTR_4g132850 29.730 259 166 6 32 287 5 250 6.76e-24 98.2
MS.gene54952.t1 MTR_1196s0010 30.605 281 158 11 18 288 263 516 2.54e-23 100
MS.gene54952.t1 MTR_3g068070 30.350 257 164 8 34 286 15 260 8.44e-23 95.5
MS.gene54952.t1 MTR_1g051165 28.625 269 169 7 32 286 12 271 1.70e-22 94.7
MS.gene54952.t1 MTR_3g068085 30.739 257 163 7 34 286 15 260 5.52e-22 93.2
MS.gene54952.t1 MTR_3g068065 30.233 258 165 7 34 287 17 263 7.02e-22 93.2
MS.gene54952.t1 MTR_3g068155 30.943 265 162 8 30 286 13 264 5.94e-21 90.5
MS.gene54952.t1 MTR_7g094520 29.963 267 154 10 34 285 11 259 1.32e-20 89.4
MS.gene54952.t1 MTR_4g058810 29.389 262 167 7 34 286 11 263 2.46e-20 89.0
MS.gene54952.t1 MTR_7g094510 29.119 261 164 8 34 285 6 254 4.75e-20 87.8
MS.gene54952.t1 MTR_1g082760 29.278 263 162 7 33 284 39 288 5.51e-20 88.2
MS.gene54952.t1 MTR_4g131740 29.545 264 161 10 33 284 39 289 1.78e-19 86.7
MS.gene54952.t1 MTR_4g058840 29.278 263 166 8 34 286 11 263 2.05e-19 86.3
MS.gene54952.t1 MTR_4g058820 29.278 263 166 8 34 286 11 263 2.05e-19 86.3
MS.gene54952.t1 MTR_7g113650 28.897 263 163 7 33 284 39 288 4.39e-19 85.9
MS.gene54952.t1 MTR_7g063390 30.739 257 167 6 34 286 16 265 2.18e-17 80.5
MS.gene54952.t1 MTR_4g097510 30.366 191 125 3 35 222 3 188 5.89e-17 79.7
MS.gene54952.t1 MTR_7g063330 28.897 263 165 8 34 287 15 264 6.82e-16 76.3
MS.gene54952.t1 MTR_7g063320 29.070 258 168 6 34 285 15 263 1.04e-15 75.9
MS.gene54952.t1 MTR_3g068175 27.626 257 171 7 34 286 17 262 1.63e-15 75.5
MS.gene54952.t1 MTR_1g068955 28.462 260 164 8 34 283 14 261 2.77e-15 74.7
MS.gene54952.t1 MTR_4g064887 26.616 263 166 6 34 286 15 260 4.27e-15 73.9
MS.gene54952.t1 MTR_8g442750 28.947 266 161 11 34 285 14 265 1.62e-14 72.8
MS.gene54952.t1 MTR_7g063360 27.413 259 173 6 34 286 16 265 1.86e-14 72.4
MS.gene54952.t1 MTR_3g068175 27.203 261 171 8 34 286 17 266 1.67e-13 69.7
MS.gene54952.t1 MTR_7g063270 27.969 261 170 9 34 287 16 265 5.00e-13 68.2
MS.gene54952.t1 MTR_4g066050 31.651 218 119 8 31 226 21 230 5.76e-13 67.8
MS.gene54952.t1 MTR_7g105560 61.538 52 20 0 32 83 11 62 3.14e-12 63.9
MS.gene54952.t1 MTR_7g063260 27.907 258 175 7 34 287 16 266 4.38e-12 65.5
MS.gene54952.t1 MTR_7g080410 26.804 194 133 4 34 220 38 229 7.48e-12 65.1
MS.gene54952.t1 MTR_7g080410 26.804 194 133 4 34 220 38 229 8.51e-12 65.1
MS.gene54952.t1 MTR_4g134660 30.047 213 131 7 29 226 1 210 7.77e-11 61.6
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene54952.t1 AT3G26760 59.197 299 118 2 1 295 1 299 1.10e-124 358
MS.gene54952.t1 AT3G26770 59.551 267 108 0 32 298 39 305 2.90e-118 342
MS.gene54952.t1 AT4G03140 55.224 268 119 1 32 299 76 342 9.56e-103 304
MS.gene54952.t1 AT3G51680 48.889 270 127 5 32 290 30 299 2.46e-77 238
MS.gene54952.t1 AT2G47120 45.725 269 131 6 31 297 3 258 5.27e-70 218
MS.gene54952.t1 AT2G47120 45.693 267 130 6 33 297 6 259 1.14e-69 217
MS.gene54952.t1 AT3G29250 42.456 285 155 5 7 289 15 292 1.89e-68 215
MS.gene54952.t1 AT2G47130 45.211 261 133 4 31 289 3 255 2.89e-68 213
MS.gene54952.t1 AT3G29260 44.828 261 134 4 31 289 3 255 1.19e-67 212
MS.gene54952.t1 AT2G47140 44.444 261 136 5 31 289 3 256 2.69e-67 211
MS.gene54952.t1 AT3G29250 44.828 261 135 5 31 289 3 256 1.95e-66 209
MS.gene54952.t1 AT3G29250 42.160 287 155 6 7 289 15 294 2.19e-66 210
MS.gene54952.t1 AT1G52340 43.071 267 133 4 32 286 16 275 1.69e-59 192
MS.gene54952.t1 AT3G42960 47.510 261 128 6 32 287 6 262 2.65e-59 191
MS.gene54952.t1 AT2G47150 31.128 257 117 7 34 289 1 198 2.90e-26 103
MS.gene54952.t1 AT5G18210 31.439 264 157 9 34 285 8 259 8.21e-26 103
MS.gene54952.t1 AT1G63380 30.075 266 161 8 34 286 19 272 2.75e-24 99.8
MS.gene54952.t1 AT1G63380 30.075 266 161 8 34 286 15 268 2.99e-24 99.8
MS.gene54952.t1 AT1G63380 30.075 266 161 8 34 286 22 275 3.52e-24 99.8
MS.gene54952.t1 AT1G63380 30.075 266 161 8 34 286 17 270 3.68e-24 99.4
MS.gene54952.t1 AT2G17845 29.658 263 164 6 34 284 47 300 3.27e-23 97.4
MS.gene54952.t1 AT1G62610 29.434 265 164 7 34 286 13 266 3.92e-23 96.7
MS.gene54952.t1 AT1G62610 29.434 265 164 7 34 286 14 267 4.46e-23 96.3
MS.gene54952.t1 AT1G62610 29.434 265 164 7 34 286 15 268 4.49e-23 96.3
MS.gene54952.t1 AT1G62610 29.434 265 164 7 34 286 19 272 4.82e-23 96.3
MS.gene54952.t1 AT3G55290 30.534 262 164 6 34 286 18 270 3.24e-22 94.4
MS.gene54952.t1 AT4G13180 30.268 261 161 9 34 285 13 261 3.27e-22 94.0
MS.gene54952.t1 AT3G55290 30.534 262 164 6 34 286 17 269 3.42e-22 94.0
MS.gene54952.t1 AT2G29150 31.818 264 163 11 34 293 16 266 4.71e-22 93.6
MS.gene54952.t1 AT1G24360 28.669 293 184 10 1 285 42 317 8.99e-22 93.6
MS.gene54952.t1 AT5G18210 31.452 248 146 9 34 269 8 243 2.54e-21 91.7
MS.gene54952.t1 AT3G03980 29.851 268 160 8 34 285 14 269 2.65e-21 91.7
MS.gene54952.t1 AT2G29340 28.788 264 172 7 34 293 7 258 1.15e-20 89.7
MS.gene54952.t1 AT2G29370 28.302 265 174 8 34 294 16 268 1.70e-20 89.4
MS.gene54952.t1 AT2G29340 28.788 264 172 7 34 293 7 258 1.97e-20 89.7
MS.gene54952.t1 AT5G06060 27.407 270 168 9 34 293 9 260 9.31e-20 87.0
MS.gene54952.t1 AT4G05530 31.439 264 155 10 32 287 7 252 2.56e-19 85.9
MS.gene54952.t1 AT3G04000 29.478 268 160 8 34 284 14 269 3.22e-19 85.9
MS.gene54952.t1 AT2G29300 27.652 264 163 8 34 286 7 253 3.42e-19 85.5
MS.gene54952.t1 AT2G29310 27.444 266 173 7 34 293 7 258 4.16e-19 85.1
MS.gene54952.t1 AT2G29300 27.652 264 163 8 34 286 7 253 6.86e-19 85.1
MS.gene54952.t1 AT2G29360 27.985 268 176 9 34 296 16 271 6.93e-19 84.7
MS.gene54952.t1 AT3G55310 29.588 267 160 8 34 286 17 269 1.18e-18 84.3
MS.gene54952.t1 AT3G05260 26.515 264 169 6 33 285 36 285 1.60e-18 84.0
MS.gene54952.t1 AT1G54870 27.652 264 165 8 33 284 83 332 1.93e-18 84.7
MS.gene54952.t1 AT1G54870 27.652 264 165 8 33 284 81 330 2.17e-18 84.3
MS.gene54952.t1 AT3G46170 28.897 263 169 6 33 286 25 278 3.60e-18 83.2
MS.gene54952.t1 AT2G29340 30.412 194 128 4 34 223 7 197 1.15e-17 80.1
MS.gene54952.t1 AT2G29340 30.412 194 128 4 34 223 7 197 1.15e-17 80.1
MS.gene54952.t1 AT2G29320 26.966 267 174 8 34 293 13 265 1.45e-17 81.3
MS.gene54952.t1 AT2G29290 28.302 265 172 10 34 293 7 258 2.63e-17 80.1
MS.gene54952.t1 AT1G10310 32.673 202 120 6 37 226 18 215 4.41e-17 79.3
MS.gene54952.t1 AT2G30670 27.273 264 176 8 34 293 7 258 1.36e-16 78.2
MS.gene54952.t1 AT2G29330 26.692 266 175 7 34 293 7 258 6.25e-16 76.3
MS.gene54952.t1 AT1G07440 25.758 264 179 7 34 293 12 262 1.78e-15 75.1
MS.gene54952.t1 AT2G29350 27.444 266 176 9 34 295 15 267 2.22e-15 75.1
MS.gene54952.t1 AT2G29260 28.996 269 172 10 34 297 68 322 2.70e-15 75.5
MS.gene54952.t1 AT2G29310 27.320 194 134 3 34 223 7 197 1.81e-14 71.2
MS.gene54952.t1 AT2G29320 27.692 195 133 4 34 223 13 204 2.87e-14 70.9
MS.gene54952.t1 AT2G29350 29.208 202 136 5 34 231 15 213 1.10e-13 69.3
MS.gene54952.t1 AT3G12800 28.364 275 170 10 34 294 10 271 6.70e-13 68.2
MS.gene54952.t1 AT1G49670 28.270 237 131 8 39 252 9 229 1.08e-12 68.9
MS.gene54952.t1 AT1G49670 28.270 237 131 8 39 252 9 229 1.25e-12 68.6
MS.gene54952.t1 AT2G29290 27.309 249 163 10 50 293 6 241 1.87e-12 66.2
MS.gene54952.t1 AT2G29310 26.033 242 160 6 51 286 22 250 1.91e-12 66.6
MS.gene54952.t1 AT5G65205 28.293 205 137 4 29 230 1 198 1.04e-11 64.7
MS.gene54952.t1 AT3G01980 24.324 259 177 6 36 284 5 254 1.41e-11 63.9
MS.gene54952.t1 AT3G01980 24.324 259 177 6 36 284 5 254 1.41e-11 63.9
MS.gene54952.t1 AT3G03330 25.888 197 132 4 34 220 41 233 1.52e-11 64.7
MS.gene54952.t1 AT5G10050 27.000 200 134 5 35 230 6 197 5.38e-11 62.4
MS.gene54952.t1 AT2G29320 25.200 250 166 8 51 293 12 247 6.52e-11 62.0

Find 89 sgRNAs with CRISPR-Local

Find 209 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CCCAATTCCTTTGCAACTTT+TGG 0.096535 7.2:+23553996 None:intergenic
GTTATGCTTGGTGGGATTAT+TGG 0.256279 7.2:+23553849 None:intergenic
CCAATGTCATTGTATCAAAT+TGG 0.270336 7.2:-23553345 MS.gene54952:CDS
AGTGGGCTATTTGGTGGGCT+TGG 0.274352 7.2:-23553486 MS.gene54952:CDS
GCCAAAAGTTGCAAAGGAAT+TGG 0.277997 7.2:-23553997 MS.gene54952:CDS
CGTTGTCGGCGATGATTACT+TGG 0.280742 7.2:+23554033 None:intergenic
GTGGGCTATTTGGTGGGCTT+GGG 0.302403 7.2:-23553485 MS.gene54952:CDS
TCAAGCATAAGTGGGCTATT+TGG 0.324040 7.2:-23553495 MS.gene54952:CDS
AGTTCACTGCTTCTTCTACT+TGG 0.342962 7.2:+23553931 None:intergenic
TATGCTTGATGTACATATAA+TGG 0.345255 7.2:+23553509 None:intergenic
CAAATTGGAAAGTTTATTCC+TGG 0.346532 7.2:-23553330 MS.gene54952:CDS
TACAACAATGCTGGCATAAC+AGG 0.355905 7.2:-23553873 MS.gene54952:CDS
AACTTTGCCATAACAAACTA+TGG 0.360430 7.2:-23553912 MS.gene54952:CDS
TAGTTTACCATAGTTTGTTA+TGG 0.381695 7.2:+23553905 None:intergenic
CCCACCAAGCATAACAGAAT+TGG 0.384770 7.2:-23553841 MS.gene54952:CDS
GTCATGAGAATCAATGTAAC+AGG 0.385275 7.2:-23553798 MS.gene54952:CDS
CCCCATGTTGGACAAATTCA+TGG 0.396340 7.2:+23554057 None:intergenic
GTTCACTGCTTCTTCTACTT+GGG 0.400670 7.2:+23553932 None:intergenic
CCAAAAGTTGCAAAGGAATT+GGG 0.408007 7.2:-23553996 MS.gene54952:CDS
CACCAACTTCAGCAATGGTT+TGG 0.409202 7.2:-23556392 MS.gene54952:CDS
TGCTAGAGCTGCATTGTACT+TGG 0.426496 7.2:-23553229 MS.gene54952:CDS
GCCGACAACGACACTAAACT+TGG 0.431149 7.2:-23554020 MS.gene54952:CDS
GTAGACTTTGATATTGTGTA+TGG 0.435479 7.2:+23553456 None:intergenic
TCCAATTCTGTTATGCTTGG+TGG 0.440019 7.2:+23553840 None:intergenic
GGTCAGAATCTCATAGTTGA+TGG 0.441728 7.2:-23553180 MS.gene54952:CDS
CCCAAGTTTAGTGTCGTTGT+CGG 0.443828 7.2:+23554019 None:intergenic
ACTGGATCAGCAAGTGGACT+TGG 0.446502 7.2:-23554092 MS.gene54952:CDS
CCAATTCTGTTATGCTTGGT+GGG 0.447299 7.2:+23553841 None:intergenic
AACCAAACCATTGCTGAAGT+TGG 0.452789 7.2:+23556390 None:intergenic
CCTAGCTAACCGATTTGATC+TGG 0.457796 7.2:+23553120 None:intergenic
GTGATGATACCAAAGGGCTA+TGG 0.461101 7.2:-23553534 MS.gene54952:CDS
GCTAGTGAACTATGTGGAGC+TGG 0.466528 7.2:-23553408 MS.gene54952:CDS
AATGTAACAGGAGTTATTGC+AGG 0.478783 7.2:-23553786 MS.gene54952:intron
GATGATGCTAAGTTTATCTC+AGG 0.492118 7.2:-23553201 MS.gene54952:CDS
GTGTGACCCTCGAACAGATA+GGG 0.495007 7.2:-23553308 MS.gene54952:CDS
CAATTTCCCCTATCTGTTCG+AGG 0.495828 7.2:+23553301 None:intergenic
AGCATAAGTGGGCTATTTGG+TGG 0.499085 7.2:-23553492 MS.gene54952:CDS
GCCCATGAATTTGTCCAACA+TGG 0.508068 7.2:-23554059 MS.gene54952:CDS
GGTGTGACCCTCGAACAGAT+AGG 0.512517 7.2:-23553309 MS.gene54952:CDS
CCAATTTGATACAATGACAT+TGG 0.512550 7.2:+23553345 None:intergenic
TGATTACTTGGGCCCCATGT+TGG 0.514016 7.2:+23554045 None:intergenic
TTTCCTTCTCCAGATCAAAT+CGG 0.524282 7.2:-23553129 MS.gene54952:CDS
ATGCAACAGTTGGTGGAAGA+AGG 0.524744 7.2:-23556348 MS.gene54952:intron
ATAATGGACCCATAGCCCTT+TGG 0.527774 7.2:+23553525 None:intergenic
CAGATAGGGGAAATTGTTAG+TGG 0.528334 7.2:-23553294 MS.gene54952:CDS
CCCATGAATTTGTCCAACAT+GGG 0.534289 7.2:-23554058 MS.gene54952:CDS
TGATGATACCAAAGGGCTAT+GGG 0.539265 7.2:-23553533 MS.gene54952:CDS
ATATGTACATCAAGCATAAG+TGG 0.539440 7.2:-23553504 MS.gene54952:CDS
TGATACAATGACATTGGTGT+AGG 0.540244 7.2:+23553351 None:intergenic
AGATTCTATGCAACAGTTGG+TGG 0.542590 7.2:-23556355 MS.gene54952:CDS
TCATGCTCAGAACACTAGCC+AGG 0.543355 7.2:-23556578 MS.gene54952:intron
ATTCCACATACTGTGCTGAA+TGG 0.544450 7.2:+23553973 None:intergenic
AATTTCCCCTATCTGTTCGA+GGG 0.544681 7.2:+23553302 None:intergenic
TTTGATATTGTGTATGGATG+AGG 0.544687 7.2:+23553462 None:intergenic
GCCACAGATTTCACCACCCC+TGG 0.546305 7.2:+23553429 None:intergenic
GACATCATGTACAACAATGC+TGG 0.549387 7.2:-23553882 MS.gene54952:CDS
ATATCAAAGTCTACAATACC+AGG 0.550206 7.2:-23553447 MS.gene54952:CDS
AGGTCCAATTCTGTTATGCT+TGG 0.553618 7.2:+23553837 None:intergenic
AAGCTCACCAACTTCAGCAA+TGG 0.557570 7.2:-23556397 MS.gene54952:CDS
GCATAAGTGGGCTATTTGGT+GGG 0.558522 7.2:-23553491 MS.gene54952:CDS
ATCAGCAAGTGGACTTGGAA+AGG 0.560560 7.2:-23554087 MS.gene54952:CDS
GGGCCATTCAGCACAGTATG+TGG 0.564489 7.2:-23553976 MS.gene54952:CDS
GGACCTTGATGAATTTGAGA+AGG 0.567216 7.2:-23553820 MS.gene54952:CDS
ATCTGTTCGAGGGTCACACC+AGG 0.568597 7.2:+23553312 None:intergenic
AGCAGATTCTATGCAACAGT+TGG 0.569130 7.2:-23556358 MS.gene54952:CDS
CCCATGTTGGACAAATTCAT+GGG 0.569913 7.2:+23554058 None:intergenic
TGACCTTCTCAAATTCATCA+AGG 0.579050 7.2:+23553817 None:intergenic
TATGTACATCAAGCATAAGT+GGG 0.587715 7.2:-23553503 MS.gene54952:CDS
TAACCGATTTGATCTGGAGA+AGG 0.590264 7.2:+23553126 None:intergenic
CTAGTGAACTATGTGGAGCT+GGG 0.592576 7.2:-23553407 MS.gene54952:CDS
GCTCGAGTGATGATACCAAA+GGG 0.594724 7.2:-23553540 MS.gene54952:CDS
CAGAATCTCATAGTTGATGG+AGG 0.597836 7.2:-23553177 MS.gene54952:CDS
ACTTGGGCCAAAAGTTGCAA+AGG 0.606003 7.2:-23554003 MS.gene54952:CDS
TGTGACCCTCGAACAGATAG+GGG 0.612645 7.2:-23553307 MS.gene54952:CDS
GTTGTCGGCGATGATTACTT+GGG 0.621879 7.2:+23554034 None:intergenic
CCATGAATTTGTCCAACATG+GGG 0.626707 7.2:-23554057 MS.gene54952:CDS
ATCATAACTGGATCAGCAAG+TGG 0.638092 7.2:-23554098 MS.gene54952:CDS
AGAATCTCATAGTTGATGGA+GGG 0.642750 7.2:-23553176 MS.gene54952:CDS
TATCAAAGTCTACAATACCA+GGG 0.649064 7.2:-23553446 MS.gene54952:CDS
AAAGTCTACAATACCAGGGG+TGG 0.651264 7.2:-23553442 MS.gene54952:CDS
GGCTCGAGTGATGATACCAA+AGG 0.651712 7.2:-23553541 MS.gene54952:CDS
CCGACAACGACACTAAACTT+GGG 0.660224 7.2:-23554019 MS.gene54952:CDS
ATCAAAGTCTACAATACCAG+GGG 0.674916 7.2:-23553445 MS.gene54952:CDS
GGAATGTGATGTCACTGTTG+AGG 0.683235 7.2:-23553955 MS.gene54952:CDS
ATTGGTGTAGGAATTGCAGT+TGG 0.686643 7.2:+23553363 None:intergenic
AGTGGACTTAGTGCTCTGAA+AGG 0.687194 7.2:-23553276 MS.gene54952:CDS
ACCAGGGGTGGTGAAATCTG+TGG 0.689503 7.2:-23553430 MS.gene54952:CDS
TCTGAAAGGTGCTAAATGTG+AGG 0.706614 7.2:-23553262 MS.gene54952:CDS
TCTGTGGCTAGTGAACTATG+TGG 0.715679 7.2:-23553414 MS.gene54952:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAATTTATTTAACATGAATT+TGG + chr7.2:23556017-23556036 None:intergenic 10.0%
!! AATTTATTTAACATGAATTT+GGG + chr7.2:23556016-23556035 None:intergenic 10.0%
!! ATATATATAGAATAATTGTT+TGG + chr7.2:23553191-23553210 None:intergenic 10.0%
!! CTTAAATACAATAAAAAAAT+TGG - chr7.2:23553726-23553745 MS.gene54952:intron 10.0%
!! AAATAAAGACAATAGAAATT+TGG - chr7.2:23554222-23554241 MS.gene54952:intron 15.0%
!! AAATAAATAATGATTGATGA+TGG + chr7.2:23555437-23555456 None:intergenic 15.0%
!! AATAAAATTGAATTAAACAG+TGG + chr7.2:23554484-23554503 None:intergenic 15.0%
!! AATTAAAAAAGAATGACTAT+TGG + chr7.2:23553160-23553179 None:intergenic 15.0%
!! ACAAAAACATAAGAAAAATA+TGG - chr7.2:23555980-23555999 MS.gene54952:intron 15.0%
!! CAAAAACATAAGAAAAATAT+GGG - chr7.2:23555981-23556000 MS.gene54952:intron 15.0%
!! CAACTGAAAATAAATAAAAA+AGG + chr7.2:23553942-23553961 None:intergenic 15.0%
!! GTTCAATTAAAAAATAAAAG+TGG + chr7.2:23554710-23554729 None:intergenic 15.0%
!! TACGAATGAAAATAAAAAAT+AGG - chr7.2:23554381-23554400 MS.gene54952:intron 15.0%
!! TCCAAAAATAAATAAACTAT+AGG - chr7.2:23553540-23553559 MS.gene54952:CDS 15.0%
!!! GTTCTTTTAAACAATTTATT+TGG - chr7.2:23554306-23554325 MS.gene54952:intron 15.0%
!!! TCATTAGTTTCATTATTTTA+TGG + chr7.2:23554421-23554440 None:intergenic 15.0%
!!! TCCTATAGTTTATTTATTTT+TGG + chr7.2:23553544-23553563 None:intergenic 15.0%
!!! TTAAATCTTTTATATATGCA+GGG - chr7.2:23553276-23553295 MS.gene54952:CDS 15.0%
!!! TTTAAATCTTTTATATATGC+AGG - chr7.2:23553275-23553294 MS.gene54952:CDS 15.0%
!! AAATCACAAAGATATAACTT+AGG + chr7.2:23556096-23556115 None:intergenic 20.0%
!! AACAAATATAATGTATGATC+AGG - chr7.2:23554545-23554564 MS.gene54952:intron 20.0%
!! AATATGCTTACAAAAATGAA+TGG + chr7.2:23554998-23555017 None:intergenic 20.0%
!! AATTATTATCTTGAAGAGAT+AGG + chr7.2:23554936-23554955 None:intergenic 20.0%
!! AGACAAGTTTATATTTGAAA+GGG - chr7.2:23555115-23555134 MS.gene54952:intron 20.0%
!! ATAAGTATTCTGTTCTATAT+AGG - chr7.2:23553220-23553239 MS.gene54952:CDS 20.0%
!! ATCTTACAAAAAAATCTTGT+TGG - chr7.2:23555303-23555322 MS.gene54952:intron 20.0%
!! ATTATTATCTTGAAGAGATA+GGG + chr7.2:23554935-23554954 None:intergenic 20.0%
!! CAATGAATCAAAAGTAATAT+AGG - chr7.2:23554086-23554105 MS.gene54952:CDS 20.0%
!! GTTTGAATCAAAAGTAATAT+AGG - chr7.2:23554127-23554146 MS.gene54952:intron 20.0%
!! TACTATAATTGCACATAATA+TGG + chr7.2:23553414-23553433 None:intergenic 20.0%
!!! ACTTTTGATTCAAACATATT+CGG + chr7.2:23554122-23554141 None:intergenic 20.0%
!!! ATACTTTAGTTTAGAAACAA+TGG - chr7.2:23554574-23554593 MS.gene54952:intron 20.0%
!!! ATTGATTAGTTTAGAAATCT+AGG + chr7.2:23554765-23554784 None:intergenic 20.0%
!!! CACTTTTTTTTTACAATGTT+TGG + chr7.2:23554795-23554814 None:intergenic 20.0%
!!! CATTCAATTCAATTCATTTT+TGG - chr7.2:23555548-23555567 MS.gene54952:intron 20.0%
!!! CTTTTAAACAATTTATTTGG+TGG - chr7.2:23554309-23554328 MS.gene54952:intron 20.0%
!!! TAAGCATTTTTATAAGCATA+TGG - chr7.2:23555025-23555044 MS.gene54952:intron 20.0%
!!! TTACTTTTCAAAGTCATTTA+AGG - chr7.2:23555272-23555291 MS.gene54952:intron 20.0%
!!! TTTGTTTTCTAGTTTCTTTT+TGG + chr7.2:23555965-23555984 None:intergenic 20.0%
! AAACATAAGAAAAATATGGG+AGG - chr7.2:23555984-23556003 MS.gene54952:intron 25.0%
! AAGAACTATAATTACCATGT+CGG + chr7.2:23554292-23554311 None:intergenic 25.0%
! AATCTTGTTGGATAAATCTT+CGG - chr7.2:23555315-23555334 MS.gene54952:intron 25.0%
! AGAATCATTAGTTGAGTTAT+TGG + chr7.2:23554737-23554756 None:intergenic 25.0%
! ATGTCAATTTAGAAGAAAGT+TGG + chr7.2:23553612-23553631 None:intergenic 25.0%
! CTAAATGTACACTAAACTAT+AGG - chr7.2:23553507-23553526 MS.gene54952:CDS 25.0%
! CTAACAAATTATCTTCACAT+AGG + chr7.2:23553822-23553841 None:intergenic 25.0%
! GACAAGTTTATATTTGAAAG+GGG - chr7.2:23555116-23555135 MS.gene54952:intron 25.0%
! GAGACAAGTTTATATTTGAA+AGG - chr7.2:23555114-23555133 MS.gene54952:intron 25.0%
! TATGCTTGATGTACATATAA+TGG + chr7.2:23556195-23556214 None:intergenic 25.0%
! TCAAATATAAACTTGTCTCA+AGG + chr7.2:23555113-23555132 None:intergenic 25.0%
! TCATTAAAGCTCTTTACTAT+GGG - chr7.2:23553385-23553404 MS.gene54952:CDS 25.0%
! TGAAAGTAACACAAATCTAA+AGG - chr7.2:23555232-23555251 MS.gene54952:intron 25.0%
! TGTCAATTTAGAAGAAAGTT+GGG + chr7.2:23553611-23553630 None:intergenic 25.0%
!! AAATGACTTTGAAAAGTAAG+AGG + chr7.2:23555271-23555290 None:intergenic 25.0%
!! GCATTACAACTTTAAGTTAA+AGG + chr7.2:23556047-23556066 None:intergenic 25.0%
!! TAAGTATATGTTTTCAACAC+AGG - chr7.2:23553780-23553799 MS.gene54952:intron 25.0%
!! TATTGTATTTAAGACAAGAG+AGG + chr7.2:23553719-23553738 None:intergenic 25.0%
!! TCATGGAGTATCAATAAAAA+TGG - chr7.2:23555166-23555185 MS.gene54952:intron 25.0%
!!! CATTTTAGGTATAAAACATG+CGG - chr7.2:23556139-23556158 MS.gene54952:intron 25.0%
!!! TAGTTTACCATAGTTTGTTA+TGG + chr7.2:23555799-23555818 None:intergenic 25.0%
!!! TCATTTCTTTTTCAACAAGT+TGG + chr7.2:23555357-23555376 None:intergenic 25.0%
AACTTTGCCATAACAAACTA+TGG - chr7.2:23555789-23555808 MS.gene54952:intron 30.0%
AAGGTCTCACTACTTTAATT+TGG + chr7.2:23555094-23555113 None:intergenic 30.0%
AATGATTATGATCCCGAATT+TGG + chr7.2:23554869-23554888 None:intergenic 30.0%
AATTTGGCTACACAACAAAA+AGG + chr7.2:23554853-23554872 None:intergenic 30.0%
ATATCAAAGTCTACAATACC+AGG - chr7.2:23556254-23556273 MS.gene54952:intron 30.0%
ATATGTACATCAAGCATAAG+TGG - chr7.2:23556197-23556216 MS.gene54952:intron 30.0%
CAAATTGGAAAGTTTATTCC+TGG - chr7.2:23556371-23556390 MS.gene54952:CDS 30.0%
CCAATGTCATTGTATCAAAT+TGG - chr7.2:23556356-23556375 MS.gene54952:CDS 30.0%
CCAATTTGATACAATGACAT+TGG + chr7.2:23556359-23556378 None:intergenic 30.0%
CTAAAATGATGAGTTTACTG+TGG + chr7.2:23556127-23556146 None:intergenic 30.0%
CTCATTAAAGCTCTTTACTA+TGG - chr7.2:23553384-23553403 MS.gene54952:CDS 30.0%
GAATGAATGAGAATCATCAT+GGG + chr7.2:23555533-23555552 None:intergenic 30.0%
GCAAAATGTCAAATAAGTAC+AGG + chr7.2:23554018-23554037 None:intergenic 30.0%
GGAAAAATTGCAATCATAAC+TGG - chr7.2:23555591-23555610 MS.gene54952:intron 30.0%
GTAGACTTTGATATTGTGTA+TGG + chr7.2:23556248-23556267 None:intergenic 30.0%
TAGTTTAGAAACAATGGCAA+CGG - chr7.2:23554580-23554599 MS.gene54952:intron 30.0%
TATCAAAGTCTACAATACCA+GGG - chr7.2:23556255-23556274 MS.gene54952:intron 30.0%
TATGTACATCAAGCATAAGT+GGG - chr7.2:23556198-23556217 MS.gene54952:intron 30.0%
TCAGTTCATTTCGGATAATT+TGG + chr7.2:23554180-23554199 None:intergenic 30.0%
TCTAAATTGACATGTATGAG+TGG - chr7.2:23553619-23553638 MS.gene54952:intron 30.0%
TGAATGAATGAGAATCATCA+TGG + chr7.2:23555534-23555553 None:intergenic 30.0%
TGCTTACAAAAATGAATGGA+TGG + chr7.2:23554994-23555013 None:intergenic 30.0%
TTGAATTCAAGTGTGTAATG+AGG + chr7.2:23554336-23554355 None:intergenic 30.0%
TTTGATATTGTGTATGGATG+AGG + chr7.2:23556242-23556261 None:intergenic 30.0%
! ACATTTAGAGTAGCATAATC+AGG + chr7.2:23553495-23553514 None:intergenic 30.0%
! AGGAAACGCGAAATTTTTAA+TGG + chr7.2:23556558-23556577 None:intergenic 30.0%
! CACAGTAAACTCATCATTTT+AGG - chr7.2:23556125-23556144 MS.gene54952:intron 30.0%
! GTGTCAAAAGTTTTACAATG+TGG + chr7.2:23553657-23553676 None:intergenic 30.0%
! GTTTATGTTTGCTAAATCCA+TGG + chr7.2:23554667-23554686 None:intergenic 30.0%
! TGGACGTAGTATTTAAAGTA+TGG + chr7.2:23554974-23554993 None:intergenic 30.0%
!! TTTTGTTGTGTAGCCAAATT+CGG - chr7.2:23554853-23554872 MS.gene54952:intron 30.0%
!!! ATTTTTTTTCCTTGATTCCC+TGG - chr7.2:23553468-23553487 MS.gene54952:CDS 30.0%
!!! TTTCTTTTTCAACAAGTTGG+AGG + chr7.2:23555354-23555373 None:intergenic 30.0%
AATGTAACAGGAGTTATTGC+AGG - chr7.2:23555915-23555934 MS.gene54952:intron 35.0%
AGAGACACGTACACTTAAAA+AGG + chr7.2:23553975-23553994 None:intergenic 35.0%
ATATGCATGTTCATATGCCA+TGG - chr7.2:23554647-23554666 MS.gene54952:intron 35.0%
ATCAAAGTCTACAATACCAG+GGG - chr7.2:23556256-23556275 MS.gene54952:intron 35.0%
CATATGAACATGCATATTGG+AGG + chr7.2:23554644-23554663 None:intergenic 35.0%
CCAAAAGTTGCAAAGGAATT+GGG - chr7.2:23555705-23555724 MS.gene54952:intron 35.0%
CTATTGGAGTATATTGAACC+TGG + chr7.2:23553144-23553163 None:intergenic 35.0%
CTCATGAGTTCAGTTCATTT+CGG + chr7.2:23554189-23554208 None:intergenic 35.0%
GATGATGCTAAGTTTATCTC+AGG - chr7.2:23556500-23556519 MS.gene54952:intron 35.0%
GTCAAATAAGTACAGGAGTT+AGG + chr7.2:23554011-23554030 None:intergenic 35.0%
GTCATGAGAATCAATGTAAC+AGG - chr7.2:23555903-23555922 MS.gene54952:intron 35.0%
TGACCTTCTCAAATTCATCA+AGG + chr7.2:23555887-23555906 None:intergenic 35.0%
TGATACAATGACATTGGTGT+AGG + chr7.2:23556353-23556372 None:intergenic 35.0%
TGGCATATGAACATGCATAT+TGG + chr7.2:23554647-23554666 None:intergenic 35.0%
TTTCCTTCTCCAGATCAAAT+CGG - chr7.2:23556572-23556591 MS.gene54952:intron 35.0%
! TTTGTTGTGTAGCCAAATTC+GGG - chr7.2:23554854-23554873 MS.gene54952:intron 35.0%
!! AGAATCTCATAGTTGATGGA+GGG - chr7.2:23556525-23556544 MS.gene54952:intron 35.0%
!! TTTCCTTCTAACCTGCTTTT+TGG + chr7.2:23555576-23555595 None:intergenic 35.0%
!!! CATTTTTGGTTCCAAAAAGC+AGG - chr7.2:23555562-23555581 MS.gene54952:intron 35.0%
AACCAAACCATTGCTGAAGT+TGG + chr7.2:23553314-23553333 None:intergenic 40.0%
AATTTCCCCTATCTGTTCGA+GGG + chr7.2:23556402-23556421 None:intergenic 40.0%
ACGGATTTCTGTGAAACACA+TGG - chr7.2:23554599-23554618 MS.gene54952:intron 40.0%
AGATTCTATGCAACAGTTGG+TGG - chr7.2:23553346-23553365 MS.gene54952:CDS 40.0%
AGCAGATTCTATGCAACAGT+TGG - chr7.2:23553343-23553362 MS.gene54952:CDS 40.0%
AGGTCCAATTCTGTTATGCT+TGG + chr7.2:23555867-23555886 None:intergenic 40.0%
AGTTCACTGCTTCTTCTACT+TGG + chr7.2:23555773-23555792 None:intergenic 40.0%
ATCATAACTGGATCAGCAAG+TGG - chr7.2:23555603-23555622 MS.gene54952:intron 40.0%
ATTCCACATACTGTGCTGAA+TGG + chr7.2:23555731-23555750 None:intergenic 40.0%
ATTGGTGTAGGAATTGCAGT+TGG + chr7.2:23556341-23556360 None:intergenic 40.0%
CAGATAGGGGAAATTGTTAG+TGG - chr7.2:23556407-23556426 MS.gene54952:CDS 40.0%
CATCCTTCATCGCTAATTGA+AGG + chr7.2:23555143-23555162 None:intergenic 40.0%
CCAATTCTGTTATGCTTGGT+GGG + chr7.2:23555863-23555882 None:intergenic 40.0%
CCATGAATTTGTCCAACATG+GGG - chr7.2:23555644-23555663 MS.gene54952:intron 40.0%
CCCATGAATTTGTCCAACAT+GGG - chr7.2:23555643-23555662 MS.gene54952:intron 40.0%
CCCATGTTGGACAAATTCAT+GGG + chr7.2:23555646-23555665 None:intergenic 40.0%
CTTCAATTAGCGATGAAGGA+TGG - chr7.2:23555141-23555160 MS.gene54952:intron 40.0%
GAAAGACAAAAGGAGTTGCT+TGG + chr7.2:23555488-23555507 None:intergenic 40.0%
GACATCATGTACAACAATGC+TGG - chr7.2:23555819-23555838 MS.gene54952:intron 40.0%
GCCAAAAGTTGCAAAGGAAT+TGG - chr7.2:23555704-23555723 MS.gene54952:intron 40.0%
GGACCTTGATGAATTTGAGA+AGG - chr7.2:23555881-23555900 MS.gene54952:intron 40.0%
GGGTGTGAGAAAAGAAAGAT+GGG + chr7.2:23555513-23555532 None:intergenic 40.0%
GGTCAGAATCTCATAGTTGA+TGG - chr7.2:23556521-23556540 MS.gene54952:intron 40.0%
GGTGTGAGAAAAGAAAGATG+GGG + chr7.2:23555512-23555531 None:intergenic 40.0%
GTGTGAGAAAAGAAAGATGG+GGG + chr7.2:23555511-23555530 None:intergenic 40.0%
GTTCACTGCTTCTTCTACTT+GGG + chr7.2:23555772-23555791 None:intergenic 40.0%
GTTCCAAAAAGCAGGTTAGA+AGG - chr7.2:23555570-23555589 MS.gene54952:intron 40.0%
TACAACAATGCTGGCATAAC+AGG - chr7.2:23555828-23555847 MS.gene54952:intron 40.0%
TCCAATTCTGTTATGCTTGG+TGG + chr7.2:23555864-23555883 None:intergenic 40.0%
TGATGATACCAAAGGGCTAT+GGG - chr7.2:23556168-23556187 MS.gene54952:intron 40.0%
TGGGTGTGAGAAAAGAAAGA+TGG + chr7.2:23555514-23555533 None:intergenic 40.0%
TGTGAGAAAAGAAAGATGGG+GGG + chr7.2:23555510-23555529 None:intergenic 40.0%
! CCCAATTCCTTTGCAACTTT+TGG + chr7.2:23555708-23555727 None:intergenic 40.0%
! GTTATGCTTGGTGGGATTAT+TGG + chr7.2:23555855-23555874 None:intergenic 40.0%
!! CAGAATCTCATAGTTGATGG+AGG - chr7.2:23556524-23556543 MS.gene54952:intron 40.0%
!! TAACCGATTTGATCTGGAGA+AGG + chr7.2:23556578-23556597 None:intergenic 40.0%
!! TCAAGCATAAGTGGGCTATT+TGG - chr7.2:23556206-23556225 MS.gene54952:intron 40.0%
!! TCTGAAAGGTGCTAAATGTG+AGG - chr7.2:23556439-23556458 MS.gene54952:intron 40.0%
!!! GCGCTTTTTTGTGTTAACTC+CGG - chr7.2:23554247-23554266 MS.gene54952:intron 40.0%
AAAGTCTACAATACCAGGGG+TGG - chr7.2:23556259-23556278 MS.gene54952:intron 45.0%
AAGATGGGGGGAAAGACAAA+AGG + chr7.2:23555498-23555517 None:intergenic 45.0%
AAGCTCACCAACTTCAGCAA+TGG - chr7.2:23553304-23553323 MS.gene54952:CDS 45.0%
ACTTGGGCCAAAAGTTGCAA+AGG - chr7.2:23555698-23555717 MS.gene54952:intron 45.0%
AGAGTAGCATAATCAGGACC+AGG + chr7.2:23553489-23553508 None:intergenic 45.0%
ATGACGACTCTCACAACGTA+AGG - chr7.2:23554152-23554171 MS.gene54952:intron 45.0%
CAATTTCCCCTATCTGTTCG+AGG + chr7.2:23556403-23556422 None:intergenic 45.0%
CACCAACTTCAGCAATGGTT+TGG - chr7.2:23553309-23553328 MS.gene54952:CDS 45.0%
CCCACCAAGCATAACAGAAT+TGG - chr7.2:23555860-23555879 MS.gene54952:intron 45.0%
CCCCATGTTGGACAAATTCA+TGG + chr7.2:23555647-23555666 None:intergenic 45.0%
CCGACAACGACACTAAACTT+GGG - chr7.2:23555682-23555701 MS.gene54952:intron 45.0%
CTAGTGAACTATGTGGAGCT+GGG - chr7.2:23556294-23556313 MS.gene54952:intron 45.0%
GAGTAGCATAATCAGGACCA+GGG + chr7.2:23553488-23553507 None:intergenic 45.0%
GCCCATGAATTTGTCCAACA+TGG - chr7.2:23555642-23555661 MS.gene54952:intron 45.0%
GCTCGAGTGATGATACCAAA+GGG - chr7.2:23556161-23556180 MS.gene54952:intron 45.0%
GGAATGTGATGTCACTGTTG+AGG - chr7.2:23555746-23555765 MS.gene54952:intron 45.0%
GGACCTTCAATTAGCGATGA+AGG - chr7.2:23555137-23555156 MS.gene54952:intron 45.0%
GTACGTGTCTCTCACTTTAG+AGG - chr7.2:23553983-23554002 MS.gene54952:CDS 45.0%
GTGATGATACCAAAGGGCTA+TGG - chr7.2:23556167-23556186 MS.gene54952:intron 45.0%
GTTGTCGGCGATGATTACTT+GGG + chr7.2:23555670-23555689 None:intergenic 45.0%
TAATCAGGACCAGGGAATCA+AGG + chr7.2:23553480-23553499 None:intergenic 45.0%
TCTGTGGCTAGTGAACTATG+TGG - chr7.2:23556287-23556306 MS.gene54952:intron 45.0%
TGCTAGAGCTGCATTGTACT+TGG - chr7.2:23556472-23556491 MS.gene54952:intron 45.0%
TGTGTTAACTCCGGATCCTA+CGG - chr7.2:23554256-23554275 MS.gene54952:intron 45.0%
! AGTGGACTTAGTGCTCTGAA+AGG - chr7.2:23556425-23556444 MS.gene54952:intron 45.0%
! ATCAGCAAGTGGACTTGGAA+AGG - chr7.2:23555614-23555633 MS.gene54952:intron 45.0%
! CAAGTTGGAGGATGATGTTG+TGG + chr7.2:23555342-23555361 None:intergenic 45.0%
! CCCAAGTTTAGTGTCGTTGT+CGG + chr7.2:23555685-23555704 None:intergenic 45.0%
!! AGCATAAGTGGGCTATTTGG+TGG - chr7.2:23556209-23556228 MS.gene54952:intron 45.0%
!! ATAATGGACCCATAGCCCTT+TGG + chr7.2:23556179-23556198 None:intergenic 45.0%
!! ATGCAACAGTTGGTGGAAGA+AGG - chr7.2:23553353-23553372 MS.gene54952:CDS 45.0%
!! GCATAAGTGGGCTATTTGGT+GGG - chr7.2:23556210-23556229 MS.gene54952:intron 45.0%
!! ATTAAAAATTAAAGAAAAAA+AGG + chr7.2:23554042-23554061 None:intergenic 5.0%
AGCGATGAAGGATGGAGTCA+TGG - chr7.2:23555149-23555168 MS.gene54952:intron 50.0%
CATATTGGAGGCAAGCTTGC+GGG + chr7.2:23554632-23554651 None:intergenic 50.0%
CGTTGTCGGCGATGATTACT+TGG + chr7.2:23555671-23555690 None:intergenic 50.0%
GCATATTGGAGGCAAGCTTG+CGG + chr7.2:23554633-23554652 None:intergenic 50.0%
GCCGACAACGACACTAAACT+TGG - chr7.2:23555681-23555700 MS.gene54952:intron 50.0%
GCTAGTGAACTATGTGGAGC+TGG - chr7.2:23556293-23556312 MS.gene54952:intron 50.0%
GGCTCGAGTGATGATACCAA+AGG - chr7.2:23556160-23556179 MS.gene54952:intron 50.0%
GTGTGACCCTCGAACAGATA+GGG - chr7.2:23556393-23556412 MS.gene54952:CDS 50.0%
GTGTTAACTCCGGATCCTAC+GGG - chr7.2:23554257-23554276 MS.gene54952:intron 50.0%
TGATTACTTGGGCCCCATGT+TGG + chr7.2:23555659-23555678 None:intergenic 50.0%
TGTGACCCTCGAACAGATAG+GGG - chr7.2:23556394-23556413 MS.gene54952:CDS 50.0%
! ACTGGATCAGCAAGTGGACT+TGG - chr7.2:23555609-23555628 MS.gene54952:intron 50.0%
AACTCCGGATCCTACGGGAA+AGG - chr7.2:23554262-23554281 MS.gene54952:intron 55.0%
ACCAGGGGTGGTGAAATCTG+TGG - chr7.2:23556271-23556290 MS.gene54952:intron 55.0%
ACTCCGGATCCTACGGGAAA+GGG - chr7.2:23554263-23554282 MS.gene54952:intron 55.0%
ATCTGTTCGAGGGTCACACC+AGG + chr7.2:23556392-23556411 None:intergenic 55.0%
GGGCCATTCAGCACAGTATG+TGG - chr7.2:23555725-23555744 MS.gene54952:intron 55.0%
GGTGTGACCCTCGAACAGAT+AGG - chr7.2:23556392-23556411 MS.gene54952:CDS 55.0%
TGTCCCTTTCCCGTAGGATC+CGG + chr7.2:23554269-23554288 None:intergenic 55.0%
!! AGTGGGCTATTTGGTGGGCT+TGG - chr7.2:23556215-23556234 MS.gene54952:intron 55.0%
!! GTGGGCTATTTGGTGGGCTT+GGG - chr7.2:23556216-23556235 MS.gene54952:intron 55.0%
ACGGGAAAGGGACACCGACA+TGG - chr7.2:23554275-23554294 MS.gene54952:intron 60.0%
GCCACAGATTTCACCACCCC+TGG + chr7.2:23556275-23556294 None:intergenic 60.0%
! TGTCGGTGTCCCTTTCCCGT+AGG + chr7.2:23554275-23554294 None:intergenic 60.0%


Chromosome Type Strat End Strand Name
chr7.2 gene 23553125 23556598 23553125 ID=MS.gene54952
chr7.2 mRNA 23553125 23556598 23553125 ID=MS.gene54952.t1;Parent=MS.gene54952
chr7.2 exon 23556579 23556598 23556579 ID=MS.gene54952.t1.exon1;Parent=MS.gene54952.t1
chr7.2 CDS 23556579 23556598 23556579 ID=cds.MS.gene54952.t1;Parent=MS.gene54952.t1
chr7.2 exon 23556349 23556426 23556349 ID=MS.gene54952.t1.exon2;Parent=MS.gene54952.t1
chr7.2 CDS 23556349 23556426 23556349 ID=cds.MS.gene54952.t1;Parent=MS.gene54952.t1
chr7.2 exon 23553787 23554139 23553787 ID=MS.gene54952.t1.exon3;Parent=MS.gene54952.t1
chr7.2 CDS 23553787 23554139 23553787 ID=cds.MS.gene54952.t1;Parent=MS.gene54952.t1
chr7.2 exon 23553125 23553576 23553125 ID=MS.gene54952.t1.exon4;Parent=MS.gene54952.t1
chr7.2 CDS 23553125 23553576 23553125 ID=cds.MS.gene54952.t1;Parent=MS.gene54952.t1
Gene Sequence

>MS.gene54952

ATGCTCAGAACACTAGCCAGGTTCAATATACTCCAATAGTCATTCTTTTTTAATTTCAAACTTCCAAACAATTATTCTATATATATTTAGTTAATATAAGTATTCTGTTCTATATAGGCTTGTCTTTAGATAAAGATTGATAAGTACTACTTTAAATCTTTTATATATGCAGGGAGTTGAAGCTCACCAACTTCAGCAATGGTTTGGTTAAAAAACGCAGCAGATTCTATGCAACAGTTGGTGGAAGAAGGTAATAACTCTCATTAAAGCTCTTTACTATGGGTATCCATATTATGTGCAATTATAGTATTATCTGAATTCTTCATAAATGAATTGCACTAGTATTTTTTTTCCTTGATTCCCTGGTCCTGATTATGCTACTCTAAATGTACACTAAACTATAGGATAAAAGTATTCCAAAAATAAATAAACTATAGGATAAAAACTAGTCTTATATTTTTTTTTTTAGCGTCTCATTTTAATCCCAACTTTCTTCTAAATTGACATGTATGAGTGGTTAGTGATTTGACCACATTGTAAAACTTTTGACACTGACAGCATATATTCAACATTAATTAAACTCTAATAATTCCTCTCTTGTCTTAAATACAATAAAAAAATTGGCACTTAGCTATAACTTGTTTCAGATTTGTGTTAAGTATATGTTTTCAACACAGGTCTGCTTAGTGAGCTACCTATGTGAAGATAATTTGTTAGCTGTCAAAATAATTTGTTTTTTTGTTTTTTAATAAAACGATGAGTAGTTAGTAAAAAATTATTCATGATTTGTTATACATATGTGAAGATAATAATACCTTTTTTATTTATTTTCAGTTGATATTATTAACCTTTTTAAGTGTACGTGTCTCTCACTTTAGAGGAGCCTAACTCCTGTACTTATTTGACATTTTGCTCCTTTTTTTCTTTAATTTTTAATATCTATTTTATTGCTATATTATAACAATGAATCAAAAGTAATATAGGTTGAGTATAGCCGAATATGTTTGAATCAAAAGTAATATAGGTTATGACGACTCTCACAACGTAAGGAACCAAATTATCCGAAATGAACTGAACTCATGAGAAACAAAATAAATAAATAAAGACAATAGAAATTTGGTTGCGCTTTTTTGTGTTAACTCCGGATCCTACGGGAAAGGGACACCGACATGGTAATTATAGTTCTTTTAAACAATTTATTTGGTGGACCTCATTACACACTTGAATTCAATTTCATTTATATACAATTTTTTTAATACGAATGAAAATAAAAAATAGGAGATGAATTAAGTACCATAAAATAATGAAACTAATGAATCACAAATTTAAAAAAAAAAAAGCAAGTGTAATTTTAGACCACTGTTTAATTCAATTTTATTTCATTTTCGTTGACAATTAAAAAGCAAGACACTGTAGTATAAACAAATATAATGTATGATCAGGAAAAATATACTTTAGTTTAGAAACAATGGCAACGGATTTCTGTGAAACACATGGAACAAAACCCGCAAGCTTGCCTCCAATATGCATGTTCATATGCCATGGATTTAGCAAACATAAACAGCTATCATCATTTAATTGTCCACTTTTATTTTTTAATTGAACGTGTCCAATAACTCAACTAATGATTCTTTTGTCCTAGATTTCTAAACTAATCAATAATGAAGCCAAACATTGTAAAAAAAAAGTGAAATCAACTTTAGTCTATTTTTATGCAAAGAAAGTCCTTTTTGTTGTGTAGCCAAATTCGGGATCATAATCATTTTATGTTGACATTTTATTTGATTTTGTTACAATTTTCTGCTATCCCTATCTCTTCAAGATAATAATTTGTGTGTCTCACAATCCATACTTTAAATACTACGTCCATCCATTCATTTTTGTAAGCATATTTAACACTTAAGCATTTTTATAAGCATATGGAGAAATCGAACATGAGATCTTGAGAGAAACACACTTCATAATTCCAAATTAAAGTAGTGAGACCTTGAGACAAGTTTATATTTGAAAGGGGACCTTCAATTAGCGATGAAGGATGGAGTCATGGAGTATCAATAAAAATGGAAAAAAGATGTCAATTTCTATATTCAAAATCAATCTTTGAACTTGAAAGTAACACAAATCTAAAGGTTCTTTTTTTCTTCCTCTTACTTTTCAAAGTCATTTAAGGAATATACAATCTTACAAAAAAATCTTGTTGGATAAATCTTCGGTCCACAACATCATCCTCCAACTTGTTGAAAAAGAAATGATGCTTTTGATTTAGACATTTGAAATAAAGCTGAAATAATTTACATTTAAATAATAAGCCATCATCAATCATTATTTATTTATTAGTGCTTCAATATTCACATTTCAAACCAAGCAACTCCTTTTGTCTTTCCCCCCATCTTTCTTTTCTCACACCCATGATGATTCTCATTCATTCAATTCAATTCATTTTTGGTTCCAAAAAGCAGGTTAGAAGGAAAAATTGCAATCATAACTGGATCAGCAAGTGGACTTGGAAAGGCAACAGCCCATGAATTTGTCCAACATGGGGCCCAAGTAATCATCGCCGACAACGACACTAAACTTGGGCCAAAAGTTGCAAAGGAATTGGGCCATTCAGCACAGTATGTGGAATGTGATGTCACTGTTGAGGCCCAAGTAGAAGAAGCAGTGAACTTTGCCATAACAAACTATGGTAAACTAGACATCATGTACAACAATGCTGGCATAACAGGACCAATAATCCCACCAAGCATAACAGAATTGGACCTTGATGAATTTGAGAAGGTCATGAGAATCAATGTAACAGGAGTTATTGCAGGTATATATTATCTTAAGAGAAAATACCAAAAAGAAACTAGAAAACAAAAACATAAGAAAAATATGGGAGGACAAAACCCAAATTCATGTTAAATAAATTTAAGATCACCTTTAACTTAAAGTTGTAATGCTTTACTTGAGAAAATTTCATATATTTCCTAAGTTATATCTTTGTGATTTAGATGTAACCACAGTAAACTCATCATTTTAGGTATAAAACATGCGGCTCGAGTGATGATACCAAAGGGCTATGGGTCCATTATATGTACATCAAGCATAAGTGGGCTATTTGGTGGGCTTGGGCCTCATCCATACACAATATCAAAGTCTACAATACCAGGGGTGGTGAAATCTGTGGCTAGTGAACTATGTGGAGCTGGGATTAGAGTCAATTGCATATCACCAACTGCAATTCCTACACCAATGTCATTGTATCAAATTGGAAAGTTTATTCCTGGTGTGACCCTCGAACAGATAGGGGAAATTGTTAGTGGACTTAGTGCTCTGAAAGGTGCTAAATGTGAGGACATTGATGTTGCTAGAGCTGCATTGTACTTGGCATCAGATGATGCTAAGTTTATCTCAGGTCAGAATCTCATAGTTGATGGAGGGTTCACTTCCATTAAAAATTTCGCGTTTCCTTCTCCAGATCAAATCGGTTAG
Protein sequence

>MS.gene54952.t1

MLRTLARELKLTNFSNGLVKKRSRFYATVGGRRLEGKIAIITGSASGLGKATAHEFVQHGAQVIIADNDTKLGPKVAKELGHSAQYVECDVTVEAQVEEAVNFAITNYGKLDIMYNNAGITGPIIPPSITELDLDEFEKVMRINVTGVIAGIKHAARVMIPKGYGSIICTSSISGLFGGLGPHPYTISKSTIPGVVKSVASELCGAGIRVNCISPTAIPTPMSLYQIGKFIPGVTLEQIGEIVSGLSALKGAKCEDIDVARAALYLASDDAKFISGQNLIVDGGFTSIKNFAFPSPDQIG