AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene58638


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene58638.t1 MTR_4g010120 91.304 46 4 0 1 46 208 253 1.06e-23 92.0
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene58638.t1 AT3G15830 69.565 46 14 0 1 46 225 270 3.75e-17 74.7
MS.gene58638.t1 AT3G15820 71.739 46 13 0 1 46 230 275 6.29e-17 74.3

Find 24 sgRNAs with CRISPR-Local

Find 84 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AGGCCATTACACTATTGATT+TGG 0.346538 2.3:-54008060 MS.gene58638:CDS
TACACTATTGATTTGGCTGT+TGG 0.354110 2.3:-54008053 MS.gene58638:CDS
AGAATTACAGTTGTAGTTGC+AGG 0.356787 2.3:+54007736 None:intergenic
ACGTTGATGGAGATCATATT+GGG 0.370369 2.3:+54006717 None:intergenic
TTTGCAAAGTGATACAGATC+TGG 0.383408 2.3:+54006877 None:intergenic
ATTGATTTGGCTGTTGGGTT+AGG 0.392652 2.3:-54008047 MS.gene58638:CDS
TATATATTTGACTCTTTAGC+TGG 0.413885 2.3:-54008017 MS.gene58638:intron
GATCTCCATCAACGTACTTC+CGG 0.414469 2.3:-54006709 MS.gene58638:CDS
TACGTTGATGGAGATCATAT+TGG 0.416147 2.3:+54006716 None:intergenic
ACACTATTGATTTGGCTGTT+GGG 0.428713 2.3:-54008052 MS.gene58638:CDS
GTAGTTGCAGGTAAGAAAAC+AGG 0.434271 2.3:+54007748 None:intergenic
TAGGGAGTGTGTTGTGTGTC+CGG 0.469707 2.3:+54006690 None:intergenic
TTGGCTGTTGGGTTAGGTGT+TGG 0.480803 2.3:-54008041 MS.gene58638:CDS
ACATAAGGAGGATGAAGAGA+TGG 0.487913 2.3:-54008145 None:intergenic
CATAAGGAGGATGAAGAGAT+GGG 0.500874 2.3:-54008144 None:intergenic
TTGCAAGCTGTGAGATTGCT+TGG 0.504819 2.3:-54008089 MS.gene58638:CDS
TTGCAAAGTGATACAGATCT+GGG 0.515328 2.3:+54006878 None:intergenic
GAGGATGAAGAGATGGGAGA+TGG 0.518694 2.3:-54008138 None:intergenic
CAGCCAAATCAATAGTGTAA+TGG 0.530095 2.3:+54008057 None:intergenic
TGAAGAGATGGGAGATGGCA+TGG 0.534871 2.3:-54008133 MS.gene58638:CDS
TGTGTCCGGAAGTACGTTGA+TGG 0.546746 2.3:+54006704 None:intergenic
GTGAGATTGCTTGGTACAAG+AGG 0.582736 2.3:-54008080 MS.gene58638:CDS
TAGACAAACATCTCAAGTGA+TGG 0.600061 2.3:-54006830 MS.gene58638:intron
CGTTGATGGAGATCATATTG+GGG 0.666163 2.3:+54006718 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATAAGTTAGAGACAAATAAA+TGG + chr2.3:54007453-54007472 None:intergenic 20.0%
!! ATAGCTAAAAATAGTATCAA+TGG - chr2.3:54006857-54006876 MS.gene58638:CDS 20.0%
!! TAAACATTATCATTTGTTCA+AGG - chr2.3:54007659-54007678 MS.gene58638:intron 20.0%
!! TTAGTGTTTAAGTGTTTATT+AGG - chr2.3:54007358-54007377 MS.gene58638:intron 20.0%
!! TTTCTATAACAGATTTAGTT+TGG - chr2.3:54007405-54007424 MS.gene58638:intron 20.0%
!!! ATTGTTTGTGCTTTTTAAAT+TGG - chr2.3:54007326-54007345 MS.gene58638:intron 20.0%
! AAAAAAGGTAACTAAACCTT+GGG - chr2.3:54007966-54007985 MS.gene58638:intron 25.0%
! AAATATTAAAGCAGTAGTGT+AGG + chr2.3:54008028-54008047 None:intergenic 25.0%
! GTTATAGAAAATATTCCCAA+AGG + chr2.3:54007395-54007414 None:intergenic 25.0%
! TAAACCAAATTATGCGAATT+AGG + chr2.3:54007701-54007720 None:intergenic 25.0%
! TATAGGCTATAGTTTATTAG+AGG - chr2.3:54007298-54007317 MS.gene58638:intron 25.0%
! TATATATTTGACTCTTTAGC+TGG - chr2.3:54006806-54006825 MS.gene58638:intron 25.0%
! TTCACTAAAAAAGATTGCAA+AGG + chr2.3:54007130-54007149 None:intergenic 25.0%
!! GATCTTTTTAAACTCTCAAA+TGG + chr2.3:54007536-54007555 None:intergenic 25.0%
!! GCATACTTAATTTGATGTTA+GGG - chr2.3:54007205-54007224 MS.gene58638:intron 25.0%
!! TTAAAAAGCACAAACAATGT+TGG + chr2.3:54007324-54007343 None:intergenic 25.0%
AAAACTGCTACTGTTATGAT+GGG - chr2.3:54007037-54007056 MS.gene58638:intron 30.0%
AAAAGGGAAACATACCAATA+AGG - chr2.3:54006930-54006949 MS.gene58638:intron 30.0%
ATAAATAGGACAAATCCACT+TGG + chr2.3:54007580-54007599 None:intergenic 30.0%
ATTTGGTTGCATGAACAATT+TGG - chr2.3:54006897-54006916 MS.gene58638:CDS 30.0%
CAAAAAAGGTAACTAAACCT+TGG - chr2.3:54007965-54007984 MS.gene58638:intron 30.0%
GTTTATGCTCAAATATGTAC+AGG - chr2.3:54007183-54007202 MS.gene58638:intron 30.0%
TAAATAGGACAAATCCACTT+GGG + chr2.3:54007579-54007598 None:intergenic 30.0%
TACACCTAATTCGCATAATT+TGG - chr2.3:54007694-54007713 MS.gene58638:intron 30.0%
TCTGTATCACTTTGCAAAAA+AGG - chr2.3:54007951-54007970 MS.gene58638:intron 30.0%
TGCTTGTCTATTATCCTTAT+TGG + chr2.3:54006947-54006966 None:intergenic 30.0%
TTTCTATGATTCTCCAGTTT+AGG + chr2.3:54007621-54007640 None:intergenic 30.0%
TTTGGTTGCATGAACAATTT+GGG - chr2.3:54006898-54006917 MS.gene58638:CDS 30.0%
! AGTGAAGTCTTGTATAAATC+AGG - chr2.3:54007144-54007163 MS.gene58638:intron 30.0%
! GGCATACTTAATTTGATGTT+AGG - chr2.3:54007204-54007223 MS.gene58638:intron 30.0%
! TTAATTTGATGTTAGGGTTG+TGG - chr2.3:54007211-54007230 MS.gene58638:intron 30.0%
! TTATTGGTATGTTTCCCTTT+TGG + chr2.3:54006931-54006950 None:intergenic 30.0%
!! ATGTTGACATTTGCTATTAG+TGG - chr2.3:54007253-54007272 MS.gene58638:intron 30.0%
!! TGTTGACATTTGCTATTAGT+GGG - chr2.3:54007254-54007273 MS.gene58638:intron 30.0%
AAACTGCTACTGTTATGATG+GGG - chr2.3:54007038-54007057 MS.gene58638:intron 35.0%
ACAATGTGTGTATGTTTGGA+TGG - chr2.3:54006968-54006987 MS.gene58638:intron 35.0%
AGAATTACAGTTGTAGTTGC+AGG + chr2.3:54007090-54007109 None:intergenic 35.0%
CAAAACTGCTACTGTTATGA+TGG - chr2.3:54007036-54007055 MS.gene58638:intron 35.0%
CAATGTGTGTATGTTTGGAT+GGG - chr2.3:54006969-54006988 MS.gene58638:intron 35.0%
CAGCCAAATCAATAGTGTAA+TGG + chr2.3:54006769-54006788 None:intergenic 35.0%
TAGACAAACATCTCAAGTGA+TGG - chr2.3:54007993-54008012 MS.gene58638:intron 35.0%
TCATGCAACCAAATCATGTT+TGG + chr2.3:54006891-54006910 None:intergenic 35.0%
TCATTCCTCCAAACATGATT+TGG - chr2.3:54006880-54006899 MS.gene58638:CDS 35.0%
TTGCAAAGTGATACAGATCT+GGG + chr2.3:54007948-54007967 None:intergenic 35.0%
TTTGCAAAGTGATACAGATC+TGG + chr2.3:54007949-54007968 None:intergenic 35.0%
! ACGTTGATGGAGATCATATT+GGG + chr2.3:54008109-54008128 None:intergenic 35.0%
! AGGCCATTACACTATTGATT+TGG - chr2.3:54006763-54006782 MS.gene58638:intron 35.0%
! GATGTTTGTCTATTTTCCCA+AGG + chr2.3:54007985-54008004 None:intergenic 35.0%
! TACGTTGATGGAGATCATAT+TGG + chr2.3:54008110-54008129 None:intergenic 35.0%
!! ACACTATTGATTTGGCTGTT+GGG - chr2.3:54006771-54006790 MS.gene58638:intron 35.0%
!! ATAGTGTTGGCACTTACTAT+AGG - chr2.3:54007281-54007300 MS.gene58638:intron 35.0%
!! TACACTATTGATTTGGCTGT+TGG - chr2.3:54006770-54006789 MS.gene58638:intron 35.0%
!!! TAGGCTGCTATTTTTCCTTT+GGG - chr2.3:54007377-54007396 MS.gene58638:intron 35.0%
!!! TTAGGCTGCTATTTTTCCTT+TGG - chr2.3:54007376-54007395 MS.gene58638:intron 35.0%
AAAATAGCATGACTTCGCAC+AGG + chr2.3:54007853-54007872 None:intergenic 40.0%
ATTAGTGGGACGTATAGTGT+TGG - chr2.3:54007268-54007287 MS.gene58638:intron 40.0%
GAAGGTGGTGTGTGATAAAT+AGG + chr2.3:54007594-54007613 None:intergenic 40.0%
GCAGACAATGTGTGTATGTT+TGG - chr2.3:54006964-54006983 MS.gene58638:intron 40.0%
GTAGTTGCAGGTAAGAAAAC+AGG + chr2.3:54007078-54007097 None:intergenic 40.0%
GTTAGGGTTGTGGAAAGATT+TGG - chr2.3:54007221-54007240 MS.gene58638:intron 40.0%
TGCAACCAAATCATGTTTGG+AGG + chr2.3:54006888-54006907 None:intergenic 40.0%
! AATTTGGGACTCTAGCCAAA+AGG - chr2.3:54006913-54006932 MS.gene58638:CDS 40.0%
! ATTTGGGACTCTAGCCAAAA+GGG - chr2.3:54006914-54006933 MS.gene58638:CDS 40.0%
! CGTTGATGGAGATCATATTG+GGG + chr2.3:54008108-54008127 None:intergenic 40.0%
!! ATTGATTTGGCTGTTGGGTT+AGG - chr2.3:54006776-54006795 MS.gene58638:intron 40.0%
!! TATTGGGGAAGTGCTAGAAA+AGG + chr2.3:54008093-54008112 None:intergenic 40.0%
CCACTTGGGTAACAACTTTG+AGG + chr2.3:54007565-54007584 None:intergenic 45.0%
CCTCAAAGTTGTTACCCAAG+TGG - chr2.3:54007562-54007581 MS.gene58638:intron 45.0%
GATCTCCATCAACGTACTTC+CGG - chr2.3:54008114-54008133 MS.gene58638:CDS 45.0%
GTGAGATTGCTTGGTACAAG+AGG - chr2.3:54006743-54006762 MS.gene58638:intron 45.0%
TGTGTGTATGTTTGGATGGG+AGG - chr2.3:54006972-54006991 MS.gene58638:intron 45.0%
TTCTCCAGTTTAGGCACAGA+AGG + chr2.3:54007612-54007631 None:intergenic 45.0%
TTGCAAGCTGTGAGATTGCT+TGG - chr2.3:54006734-54006753 MS.gene58638:intron 45.0%
! AGGTGATTTTGTCGACGACA+TGG - chr2.3:54006992-54007011 MS.gene58638:intron 45.0%
! GGTAACAACTTTGAGGACTC+AGG + chr2.3:54007558-54007577 None:intergenic 45.0%
! TGATTTTGTCGACGACATGG+TGG - chr2.3:54006995-54007014 MS.gene58638:intron 45.0%
!! TCTTAAAAAAAAAAAAAAAA+AGG + chr2.3:54007731-54007750 None:intergenic 5.0%
!!! TTTTATATCATAATTTTTTT+TGG - chr2.3:54007775-54007794 MS.gene58638:intron 5.0%
ACCACCTTCTGTGCCTAAAC+TGG - chr2.3:54007605-54007624 MS.gene58638:intron 50.0%
TCCAGTTTAGGCACAGAAGG+TGG + chr2.3:54007609-54007628 None:intergenic 50.0%
TGAAGAGATGGGAGATGGCA+TGG - chr2.3:54006690-54006709 MS.gene58638:CDS 50.0%
! TAGGGAGTGTGTTGTGTGTC+CGG + chr2.3:54008136-54008155 None:intergenic 50.0%
! TGTGTCCGGAAGTACGTTGA+TGG + chr2.3:54008122-54008141 None:intergenic 50.0%
! TTGGCTGTTGGGTTAGGTGT+TGG - chr2.3:54006782-54006801 MS.gene58638:intron 50.0%


Chromosome Type Strat End Strand Name
chr2.3 gene 54006689 54008156 54006689 ID=MS.gene58638
chr2.3 mRNA 54006689 54008156 54006689 ID=MS.gene58638.t1;Parent=MS.gene58638
chr2.3 exon 54008018 54008156 54008018 ID=MS.gene58638.t1.exon1;Parent=MS.gene58638.t1
chr2.3 CDS 54008018 54008156 54008018 ID=cds.MS.gene58638.t1;Parent=MS.gene58638.t1
chr2.3 exon 54007744 54007772 54007744 ID=MS.gene58638.t1.exon2;Parent=MS.gene58638.t1
chr2.3 CDS 54007744 54007772 54007744 ID=cds.MS.gene58638.t1;Parent=MS.gene58638.t1
chr2.3 exon 54006831 54006935 54006831 ID=MS.gene58638.t1.exon3;Parent=MS.gene58638.t1
chr2.3 CDS 54006831 54006935 54006831 ID=cds.MS.gene58638.t1;Parent=MS.gene58638.t1
chr2.3 exon 54006689 54006745 54006689 ID=MS.gene58638.t1.exon4;Parent=MS.gene58638.t1
chr2.3 CDS 54006689 54006745 54006689 ID=cds.MS.gene58638.t1;Parent=MS.gene58638.t1
Gene Sequence

>MS.gene58638

ATGAAGAGATGGGAGATGGCATGGACTTTTGATTTATTGAATGTGTTGCAAGCTGTGAGATTGCTTGGTACAAGAGGCCATTACACTATTGATTTGGCTGTTGGGTTAGGTGTTGGCTATATATTTGACTCTTTAGCTGGTAAGTATCTTGAAGATAGCAAGTTGAAAATAGCTAAAAATAGTATCAATGGTCATTCCTCCAAACATGATTTGGTTGCATGAACAATTTGGGACTCTAGCCAAAAGGGAAACATACCAATAAGGATAATAGACAAGCAGACAATGTGTGTATGTTTGGATGGGAGGTGATTTTGTCGACGACATGGTGGATATGATAAACTATGATACAAAACTGCTACTGTTATGATGGGGTACATGAATAAGAACCTGTTTTCTTACCTGCAACTACAACTGTAATTCTTTTTAGTCTGAATATGTCCTTTGCAATCTTTTTTAGTGAAGTCTTGTATAAATCAGGACATTAGATACACTTTGTTTATGCTCAAATATGTACAGGCATACTTAATTTGATGTTAGGGTTGTGGAAAGATTTGGTAGACTATAATGTTGACATTTGCTATTAGTGGGACGTATAGTGTTGGCACTTACTATAGGCTATAGTTTATTAGAGGCCAACATTGTTTGTGCTTTTTAAATTGGTTTGATATCTTAGTGTTTAAGTGTTTATTAGGCTGCTATTTTTCCTTTGGGAATATTTTCTATAACAGATTTAGTTTGGTTTCATATATCTTGATGTTAAACCATTTATTTGTCTCTAACTTATGAAGTGTGTGAATTAAGATCTATCTTGATGTTAAATCATTTATTTATCTTTAAATTCTGTCCATTTGAGAGTTTAAAAAGATCCTGAGTCCTCAAAGTTGTTACCCAAGTGGATTTGTCCTATTTATCACACACCACCTTCTGTGCCTAAACTGGAGAATCATAGAAACAGTCTTATAGAAGACTTTAAACATTATCATTTGTTCAAGGTTTAGTCTTTTTTACACCTAATTCGCATAATTTGGTTTAGTTTTTACCTTTTTTTTTTTTTTTTTAAGATCTTAGTCAAACTTACTAATTTTTTTTTATATCATAATTTTTTTTGGAATCATAATTTTTTACTTGAGAACAATTTTTTTTATAAAATACGTAATTCAACCTGTGCGAAGTCATGCTATTTTTTTAAAATAACTTATGCGAATTATAAAAATTACTCAGATTCAGAAGATGATGAAGACTATTACGATTTACGACCCAGATCTGTATCACTTTGCAAAAAAGGTAACTAAACCTTGGGAAAATAGACAAACATCTCAAGTGATGGTAAGTAACGCCTACACTACTGCTTTAATATTTAATCTTTCTAATCGTAGTCATTAACTAAATCATATTACTATTCCTTTTCTAGCACTTCCCCAATATGATCTCCATCAACGTACTTCCGGACACACAACACACTCCCTAA

Protein sequence

>MS.gene58638.t1

MKRWEMAWTFDLLNVLQAVRLLGTRGHYTIDLAVGLGVGYIFDSLAEPVFLPATTTIQKMMKTITIYDPDLYHFAKKVTKPWENRQTSQVMHFPNMISINVLPDTQHTP