Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene99544.t1 | XP_003628224.1 | 95.4 | 218 | 10 | 0 | 1 | 218 | 19 | 236 | 1.00E-118 | 436 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene99544.t1 | Q43675 | 54.0 | 198 | 78 | 4 | 13 | 204 | 68 | 258 | 2.4e-52 | 206.8 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene99544.t1 | G7L8H6 | 95.4 | 218 | 10 | 0 | 1 | 218 | 19 | 236 | 7.2e-119 | 436.0 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049993 | MS.gene99544 | -0.866262 | 3.24E-65 | -1.69E-46 |
| MS.gene050060 | MS.gene99544 | -0.802587 | 5.23E-49 | -1.69E-46 |
| MS.gene050301 | MS.gene99544 | -0.803407 | 3.54E-49 | -1.69E-46 |
| MS.gene050397 | MS.gene99544 | -0.842223 | 2.95E-58 | -1.69E-46 |
| MS.gene050406 | MS.gene99544 | -0.82093 | 5.29E-53 | -1.69E-46 |
| MS.gene050426 | MS.gene99544 | 0.841396 | 4.87E-58 | -1.69E-46 |
| MS.gene050464 | MS.gene99544 | -0.841978 | 3.42E-58 | -1.69E-46 |
| MS.gene050529 | MS.gene99544 | -0.840967 | 6.32E-58 | -1.69E-46 |
| MS.gene050642 | MS.gene99544 | -0.80614 | 9.51E-50 | -1.69E-46 |
| MS.gene050670 | MS.gene99544 | -0.808715 | 2.70E-50 | -1.69E-46 |
| MS.gene050971 | MS.gene99544 | -0.80658 | 7.68E-50 | -1.69E-46 |
| MS.gene051158 | MS.gene99544 | 0.848012 | 8.04E-60 | -1.69E-46 |
| MS.gene051525 | MS.gene99544 | -0.823057 | 1.70E-53 | -1.69E-46 |
| MS.gene051860 | MS.gene99544 | -0.835731 | 1.41E-56 | -1.69E-46 |
| MS.gene051983 | MS.gene99544 | -0.822406 | 2.41E-53 | -1.69E-46 |
| MS.gene051996 | MS.gene99544 | 0.804251 | 2.36E-49 | -1.69E-46 |
| MS.gene052011 | MS.gene99544 | 0.87026 | 1.67E-66 | -1.69E-46 |
| MS.gene052077 | MS.gene99544 | -0.820332 | 7.26E-53 | -1.69E-46 |
| MS.gene052134 | MS.gene99544 | -0.806313 | 8.74E-50 | -1.69E-46 |
| MS.gene052135 | MS.gene99544 | -0.8092 | 2.13E-50 | -1.69E-46 |
| MS.gene052178 | MS.gene99544 | -0.809435 | 1.90E-50 | -1.69E-46 |
| MS.gene052188 | MS.gene99544 | -0.804169 | 2.46E-49 | -1.69E-46 |
| MS.gene052223 | MS.gene99544 | 0.85643 | 3.24E-62 | -1.69E-46 |
| MS.gene052253 | MS.gene99544 | -0.840699 | 7.43E-58 | -1.69E-46 |
| MS.gene05233 | MS.gene99544 | 0.840471 | 8.53E-58 | -1.69E-46 |
| MS.gene052340 | MS.gene99544 | -0.827855 | 1.24E-54 | -1.69E-46 |
| MS.gene052758 | MS.gene99544 | -0.800025 | 1.75E-48 | -1.69E-46 |
| MS.gene053330 | MS.gene99544 | -0.803531 | 3.34E-49 | -1.69E-46 |
| MS.gene053406 | MS.gene99544 | 0.818221 | 2.20E-52 | -1.69E-46 |
| MS.gene053557 | MS.gene99544 | -0.819993 | 8.68E-53 | -1.69E-46 |
| MS.gene053836 | MS.gene99544 | -0.83941 | 1.61E-57 | -1.69E-46 |
| MS.gene054010 | MS.gene99544 | -0.817631 | 2.98E-52 | -1.69E-46 |
| MS.gene054011 | MS.gene99544 | -0.819275 | 1.27E-52 | -1.69E-46 |
| MS.gene054012 | MS.gene99544 | -0.821709 | 3.50E-53 | -1.69E-46 |
| MS.gene054013 | MS.gene99544 | -0.814023 | 1.90E-51 | -1.69E-46 |
| MS.gene054183 | MS.gene99544 | -0.862988 | 3.43E-64 | -1.69E-46 |
| MS.gene054277 | MS.gene99544 | 0.826827 | 2.19E-54 | -1.69E-46 |
| MS.gene054349 | MS.gene99544 | -0.816208 | 6.23E-52 | -1.69E-46 |
| MS.gene054742 | MS.gene99544 | 0.837305 | 5.62E-57 | -1.69E-46 |
| MS.gene055116 | MS.gene99544 | -0.810523 | 1.11E-50 | -1.69E-46 |
| MS.gene055157 | MS.gene99544 | 0.80077 | 1.23E-48 | -1.69E-46 |
| MS.gene055277 | MS.gene99544 | -0.827411 | 1.59E-54 | -1.69E-46 |
| MS.gene055369 | MS.gene99544 | -0.804778 | 1.83E-49 | -1.69E-46 |
| MS.gene055422 | MS.gene99544 | -0.806309 | 8.76E-50 | -1.69E-46 |
| MS.gene055428 | MS.gene99544 | -0.801929 | 7.14E-49 | -1.69E-46 |
| MS.gene05553 | MS.gene99544 | -0.825224 | 5.27E-54 | -1.69E-46 |
| MS.gene055595 | MS.gene99544 | 0.849177 | 3.82E-60 | -1.69E-46 |
| MS.gene055597 | MS.gene99544 | 0.843624 | 1.25E-58 | -1.69E-46 |
| MS.gene055601 | MS.gene99544 | 0.883191 | 5.53E-71 | -1.69E-46 |
| MS.gene055604 | MS.gene99544 | -0.81455 | 1.46E-51 | -1.69E-46 |
| MS.gene056191 | MS.gene99544 | -0.810659 | 1.03E-50 | -1.69E-46 |
| MS.gene056330 | MS.gene99544 | 0.861986 | 6.98E-64 | -1.69E-46 |
| MS.gene056612 | MS.gene99544 | -0.825305 | 5.04E-54 | -1.69E-46 |
| MS.gene056659 | MS.gene99544 | -0.801467 | 8.88E-49 | -1.69E-46 |
| MS.gene056690 | MS.gene99544 | 0.860059 | 2.69E-63 | -1.69E-46 |
| MS.gene056691 | MS.gene99544 | 0.845644 | 3.57E-59 | -1.69E-46 |
| MS.gene056723 | MS.gene99544 | -0.816281 | 6.00E-52 | -1.69E-46 |
| MS.gene056899 | MS.gene99544 | 0.903795 | 2.40E-79 | -1.69E-46 |
| MS.gene056920 | MS.gene99544 | 0.834219 | 3.40E-56 | -1.69E-46 |
| MS.gene056923 | MS.gene99544 | 0.822895 | 1.85E-53 | -1.69E-46 |
| MS.gene056924 | MS.gene99544 | 0.822235 | 2.64E-53 | -1.69E-46 |
| MS.gene056979 | MS.gene99544 | -0.815315 | 9.85E-52 | -1.69E-46 |
| MS.gene05702 | MS.gene99544 | -0.819594 | 1.07E-52 | -1.69E-46 |
| MS.gene057396 | MS.gene99544 | -0.831017 | 2.12E-55 | -1.69E-46 |
| MS.gene057476 | MS.gene99544 | -0.801919 | 7.17E-49 | -1.69E-46 |
| MS.gene057749 | MS.gene99544 | -0.814552 | 1.45E-51 | -1.69E-46 |
| MS.gene057823 | MS.gene99544 | -0.810664 | 1.03E-50 | -1.69E-46 |
| MS.gene058002 | MS.gene99544 | -0.826084 | 3.29E-54 | -1.69E-46 |
| MS.gene05811 | MS.gene99544 | -0.824087 | 9.76E-54 | -1.69E-46 |
| MS.gene058142 | MS.gene99544 | -0.812316 | 4.51E-51 | -1.69E-46 |
| MS.gene058218 | MS.gene99544 | 0.863628 | 2.17E-64 | -1.69E-46 |
| MS.gene058233 | MS.gene99544 | -0.802163 | 6.39E-49 | -1.69E-46 |
| MS.gene058276 | MS.gene99544 | -0.801804 | 7.58E-49 | -1.69E-46 |
| MS.gene058660 | MS.gene99544 | -0.802479 | 5.50E-49 | -1.69E-46 |
| MS.gene058928 | MS.gene99544 | -0.8141 | 1.83E-51 | -1.69E-46 |
| MS.gene059011 | MS.gene99544 | -0.804364 | 2.24E-49 | -1.69E-46 |
| MS.gene059043 | MS.gene99544 | 0.870685 | 1.21E-66 | -1.69E-46 |
| MS.gene05907 | MS.gene99544 | -0.837882 | 4.00E-57 | -1.69E-46 |
| MS.gene059294 | MS.gene99544 | -0.842254 | 2.89E-58 | -1.69E-46 |
| MS.gene059420 | MS.gene99544 | -0.856802 | 2.52E-62 | -1.69E-46 |
| MS.gene05984 | MS.gene99544 | -0.838883 | 2.21E-57 | -1.69E-46 |
| MS.gene059894 | MS.gene99544 | -0.81742 | 3.33E-52 | -1.69E-46 |
| MS.gene060087 | MS.gene99544 | 0.821454 | 4.00E-53 | -1.69E-46 |
| MS.gene060247 | MS.gene99544 | -0.800205 | 1.61E-48 | -1.69E-46 |
| MS.gene061049 | MS.gene99544 | -0.847121 | 1.41E-59 | -1.69E-46 |
| MS.gene061114 | MS.gene99544 | 0.81037 | 1.19E-50 | -1.69E-46 |
| MS.gene061154 | MS.gene99544 | -0.806462 | 8.13E-50 | -1.69E-46 |
| MS.gene061159 | MS.gene99544 | -0.820174 | 7.88E-53 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene99544.t1 | MTR_8g046000 | 95.413 | 218 | 10 | 0 | 1 | 218 | 19 | 236 | 1.15e-159 | 441 |
| MS.gene99544.t1 | MTR_8g045890 | 66.667 | 162 | 54 | 0 | 57 | 218 | 284 | 445 | 3.34e-76 | 236 |
| MS.gene99544.t1 | MTR_8g445140 | 50.820 | 244 | 68 | 4 | 13 | 204 | 59 | 302 | 1.08e-75 | 231 |
| MS.gene99544.t1 | MTR_8g045890 | 66.667 | 162 | 54 | 0 | 57 | 218 | 325 | 486 | 1.45e-75 | 236 |
| MS.gene99544.t1 | MTR_8g045890 | 66.667 | 162 | 54 | 0 | 57 | 218 | 362 | 523 | 3.14e-75 | 236 |
| MS.gene99544.t1 | MTR_8g445170 | 62.500 | 176 | 61 | 2 | 43 | 218 | 227 | 397 | 9.85e-75 | 231 |
| MS.gene99544.t1 | MTR_8g045880 | 61.458 | 192 | 66 | 2 | 35 | 218 | 137 | 328 | 7.23e-74 | 227 |
| MS.gene99544.t1 | MTR_8g045880 | 61.458 | 192 | 66 | 2 | 35 | 218 | 172 | 363 | 2.25e-73 | 226 |
| MS.gene99544.t1 | MTR_8g045880 | 61.458 | 192 | 66 | 2 | 35 | 218 | 174 | 365 | 3.41e-73 | 226 |
| MS.gene99544.t1 | MTR_1g045860 | 56.667 | 210 | 78 | 6 | 13 | 218 | 69 | 269 | 3.96e-73 | 223 |
| MS.gene99544.t1 | MTR_2g081770 | 49.490 | 196 | 91 | 4 | 13 | 204 | 79 | 270 | 1.17e-60 | 191 |
| MS.gene99544.t1 | MTR_2g081610 | 45.918 | 196 | 97 | 3 | 11 | 204 | 71 | 259 | 9.36e-60 | 189 |
| MS.gene99544.t1 | MTR_2g081590 | 46.392 | 194 | 95 | 3 | 13 | 204 | 73 | 259 | 2.36e-57 | 182 |
| MS.gene99544.t1 | MTR_8g044290 | 61.224 | 147 | 57 | 0 | 35 | 181 | 197 | 343 | 6.00e-56 | 184 |
| MS.gene99544.t1 | MTR_3g109490 | 34.956 | 226 | 114 | 8 | 7 | 204 | 101 | 321 | 1.23e-34 | 125 |
| MS.gene99544.t1 | MTR_1g008460 | 35.681 | 213 | 111 | 7 | 12 | 203 | 88 | 295 | 1.32e-34 | 125 |
| MS.gene99544.t1 | MTR_1g008470 | 38.532 | 218 | 101 | 9 | 12 | 203 | 88 | 298 | 9.47e-34 | 122 |
| MS.gene99544.t1 | MTR_1g008590 | 38.073 | 218 | 103 | 9 | 12 | 203 | 112 | 323 | 4.70e-33 | 121 |
| MS.gene99544.t1 | MTR_0312s0030 | 38.073 | 218 | 103 | 9 | 12 | 203 | 112 | 323 | 8.30e-33 | 120 |
| MS.gene99544.t1 | MTR_1g007520 | 38.073 | 218 | 103 | 9 | 12 | 203 | 112 | 323 | 8.30e-33 | 120 |
| MS.gene99544.t1 | MTR_1g008560 | 37.500 | 216 | 110 | 8 | 12 | 203 | 114 | 328 | 8.11e-32 | 118 |
| MS.gene99544.t1 | MTR_1g008580 | 43.066 | 137 | 71 | 4 | 74 | 203 | 34 | 170 | 1.45e-31 | 113 |
| MS.gene99544.t1 | MTR_1g008510 | 37.963 | 216 | 105 | 9 | 12 | 203 | 114 | 324 | 1.80e-31 | 117 |
| MS.gene99544.t1 | MTR_1g008530 | 40.972 | 144 | 78 | 4 | 67 | 203 | 79 | 222 | 1.91e-31 | 114 |
| MS.gene99544.t1 | MTR_1g008500 | 35.000 | 220 | 109 | 8 | 12 | 203 | 170 | 383 | 4.92e-31 | 117 |
| MS.gene99544.t1 | MTR_1g008450 | 41.481 | 135 | 74 | 4 | 74 | 203 | 177 | 311 | 2.28e-26 | 103 |
| MS.gene99544.t1 | MTR_3g116270 | 31.193 | 218 | 124 | 7 | 13 | 206 | 292 | 507 | 8.50e-23 | 95.9 |
| MS.gene99544.t1 | MTR_3g116320 | 32.110 | 218 | 122 | 7 | 13 | 206 | 474 | 689 | 1.04e-22 | 95.9 |
| MS.gene99544.t1 | MTR_3g107830 | 39.865 | 148 | 79 | 5 | 67 | 204 | 108 | 255 | 1.86e-22 | 92.4 |
| MS.gene99544.t1 | MTR_4g069680 | 38.514 | 148 | 81 | 6 | 67 | 204 | 139 | 286 | 2.36e-20 | 87.4 |
| MS.gene99544.t1 | MTR_3g116410 | 38.514 | 148 | 81 | 5 | 67 | 204 | 128 | 275 | 8.22e-20 | 85.5 |
| MS.gene99544.t1 | MTR_4g069570 | 37.013 | 154 | 83 | 6 | 65 | 205 | 184 | 336 | 1.65e-18 | 82.8 |
| MS.gene99544.t1 | MTR_3g116380 | 35.065 | 154 | 90 | 6 | 61 | 205 | 151 | 303 | 3.60e-18 | 81.3 |
| MS.gene99544.t1 | MTR_4g069040 | 28.899 | 218 | 127 | 9 | 13 | 204 | 294 | 509 | 1.03e-16 | 78.6 |
| MS.gene99544.t1 | MTR_4g069550 | 30.719 | 153 | 90 | 6 | 67 | 205 | 313 | 463 | 1.26e-12 | 66.6 |
| MS.gene99544.t1 | MTR_5g033350 | 30.968 | 155 | 96 | 5 | 57 | 202 | 46 | 198 | 2.63e-12 | 63.9 |
| MS.gene99544.t1 | MTR_4g133840 | 32.298 | 161 | 97 | 7 | 52 | 202 | 28 | 186 | 5.98e-12 | 62.4 |
| MS.gene99544.t1 | MTR_5g033340 | 30.968 | 155 | 96 | 5 | 57 | 202 | 225 | 377 | 1.02e-11 | 63.5 |
| MS.gene99544.t1 | MTR_4g069520 | 26.941 | 219 | 132 | 9 | 13 | 205 | 507 | 723 | 2.23e-11 | 63.2 |
| MS.gene99544.t1 | MTR_4g069520 | 26.941 | 219 | 132 | 9 | 13 | 205 | 493 | 709 | 2.79e-11 | 62.8 |
| MS.gene99544.t1 | MTR_4g068420 | 31.343 | 134 | 73 | 6 | 78 | 205 | 1 | 121 | 3.62e-11 | 58.9 |
| MS.gene99544.t1 | MTR_5g033330 | 30.323 | 155 | 97 | 5 | 57 | 202 | 464 | 616 | 5.65e-11 | 61.6 |
| MS.gene99544.t1 | MTR_6g086530 | 31.469 | 143 | 86 | 6 | 74 | 204 | 63 | 205 | 8.40e-11 | 59.7 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene99544.t1 | AT5G25610 | 37.946 | 224 | 109 | 9 | 7 | 203 | 170 | 390 | 3.11e-32 | 120 |
| MS.gene99544.t1 | AT1G70370 | 32.192 | 146 | 87 | 5 | 67 | 202 | 479 | 622 | 5.37e-13 | 67.8 |
| MS.gene99544.t1 | AT1G70370 | 32.192 | 146 | 87 | 5 | 67 | 202 | 479 | 622 | 5.37e-13 | 67.8 |
| MS.gene99544.t1 | AT1G49320 | 27.434 | 226 | 126 | 9 | 12 | 203 | 57 | 278 | 4.41e-12 | 64.3 |
Find 46 sgRNAs with CRISPR-Local
Find 53 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AGAACTTCATCAAGCAAATT+TGG | 0.152897 | 8.1:-59515497 | None:intergenic |
| GTTGAATATTGGAAAGCTTT+TGG | 0.243886 | 8.1:-59515058 | None:intergenic |
| ACGAAAATTAAGTCATATTC+AGG | 0.260765 | 8.1:+59515260 | MS.gene99544:CDS |
| TGTTCGGAATGGTTGAATAT+TGG | 0.268442 | 8.1:-59515069 | None:intergenic |
| GGCAAATTCCTTCATTGATT+CGG | 0.336511 | 8.1:-59515222 | None:intergenic |
| AACTTACATGGTTCCATTGT+TGG | 0.360469 | 8.1:+59515412 | MS.gene99544:CDS |
| GATTCACTTGCAACAGGAAT+AGG | 0.379182 | 8.1:-59515584 | None:intergenic |
| TGGCAGACTGGGATAGTTCC+AGG | 0.384913 | 8.1:-59515530 | None:intergenic |
| CAGCCTTGTTACCAACAAAA+TGG | 0.391110 | 8.1:-59515550 | None:intergenic |
| ATTCAACCATTCCGAACAAT+AGG | 0.392465 | 8.1:+59515074 | MS.gene99544:CDS |
| TTTAATAATGCAGACATTGA+AGG | 0.399608 | 8.1:+59514981 | MS.gene99544:CDS |
| GATTCATACCTCTTGTATCA+TGG | 0.417147 | 8.1:-59515475 | None:intergenic |
| ATATCCAAATATCATCTAGA+TGG | 0.433321 | 8.1:-59515624 | None:intergenic |
| TTACTTCTTCCACTACATAT+TGG | 0.433811 | 8.1:-59515304 | None:intergenic |
| GTTCCATTGTTGGCCTCTGA+TGG | 0.457551 | 8.1:+59515422 | MS.gene99544:CDS |
| TATAGTCTTGACGAGATTTG+TGG | 0.471610 | 8.1:+59515155 | MS.gene99544:CDS |
| TCATCAGATTCACTTGCAAC+AGG | 0.481559 | 8.1:-59515590 | None:intergenic |
| AACTTGTCTTCTCCGATAGC+AGG | 0.508034 | 8.1:-59515185 | None:intergenic |
| TTGGAAAATGACCTATACCC+TGG | 0.518567 | 8.1:+59515017 | MS.gene99544:CDS |
| TCGGCAACCATCCTATTGTT+CGG | 0.521145 | 8.1:-59515085 | None:intergenic |
| AACCATCCTATTGTTCGGAA+TGG | 0.524994 | 8.1:-59515080 | None:intergenic |
| AACATCCTCCGAATCAATGA+AGG | 0.526354 | 8.1:+59515214 | MS.gene99544:CDS |
| GAAGTTCTCAAAGTCAAGCC+TGG | 0.528095 | 8.1:+59515512 | MS.gene99544:CDS |
| GAATTTGCCATTTCAAAACT+CGG | 0.533199 | 8.1:+59515236 | MS.gene99544:CDS |
| AATTTGCCATTTCAAAACTC+GGG | 0.540236 | 8.1:+59515237 | MS.gene99544:CDS |
| TTGGTAACAAGGCTGTTGCT+TGG | 0.540533 | 8.1:+59515558 | MS.gene99544:CDS |
| TGTGGAGGTCCACCTGCTAT+CGG | 0.544677 | 8.1:+59515173 | MS.gene99544:CDS |
| TACCAACAAAATGGCAGACT+GGG | 0.549171 | 8.1:-59515541 | None:intergenic |
| TTACCAACAAAATGGCAGAC+TGG | 0.565164 | 8.1:-59515542 | None:intergenic |
| TTGTTGATGCATTCACTTGG+TGG | 0.566717 | 8.1:-59515391 | None:intergenic |
| GAATGCATCAACAACTTACA+TGG | 0.567371 | 8.1:+59515400 | MS.gene99544:CDS |
| AAATTCCTTCATTGATTCGG+AGG | 0.568883 | 8.1:-59515219 | None:intergenic |
| GTAAAGAAAATTGCAGACAA+AGG | 0.575220 | 8.1:+59515323 | MS.gene99544:CDS |
| TGGAACTAAAGTTAATGCAT+TGG | 0.576995 | 8.1:+59515442 | MS.gene99544:CDS |
| ATTAACTTTAGTTCCATCAG+AGG | 0.588740 | 8.1:-59515435 | None:intergenic |
| GTTCCATCAGAGGCCAACAA+TGG | 0.589021 | 8.1:-59515425 | None:intergenic |
| TTCTCCACTACATTTCCAGA+CGG | 0.593237 | 8.1:-59515125 | None:intergenic |
| AACCATTCCGAACAATAGGA+TGG | 0.593760 | 8.1:+59515078 | MS.gene99544:CDS |
| AAATCAAGACCAATATGTAG+TGG | 0.595851 | 8.1:+59515295 | MS.gene99544:CDS |
| AGTCTTGACGAGATTTGTGG+AGG | 0.619391 | 8.1:+59515158 | MS.gene99544:CDS |
| TTGTCTTCTCCGATAGCAGG+TGG | 0.637131 | 8.1:-59515182 | None:intergenic |
| TCAACCGTCTGGAAATGTAG+TGG | 0.641252 | 8.1:+59515121 | MS.gene99544:CDS |
| TACCCTGGCAAGAAAATGAG+CGG | 0.655096 | 8.1:+59515032 | MS.gene99544:CDS |
| AAGTTGTTGATGCATTCACT+TGG | 0.662304 | 8.1:-59515394 | None:intergenic |
| GCTTGTCACCATGATACAAG+AGG | 0.667198 | 8.1:+59515467 | MS.gene99544:CDS |
| AATATCATCTAGATGGCACA+AGG | 0.675245 | 8.1:-59515617 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | ACGAAAATTAAGTCATATTC+AGG | + | chr8.1:59515260-59515279 | MS.gene99544:CDS | 25.0% |
| ! | TTTAATAATGCAGACATTGA+AGG | + | chr8.1:59514981-59515000 | MS.gene99544:CDS | 25.0% |
| AAATCAAGACCAATATGTAG+TGG | + | chr8.1:59515295-59515314 | MS.gene99544:CDS | 30.0% | |
| AGAACTTCATCAAGCAAATT+TGG | - | chr8.1:59515500-59515519 | None:intergenic | 30.0% | |
| GTAAAGAAAATTGCAGACAA+AGG | + | chr8.1:59515323-59515342 | MS.gene99544:CDS | 30.0% | |
| TGGAACTAAAGTTAATGCAT+TGG | + | chr8.1:59515442-59515461 | MS.gene99544:CDS | 30.0% | |
| TTACTTCTTCCACTACATAT+TGG | - | chr8.1:59515307-59515326 | None:intergenic | 30.0% | |
| ! | AATTTGCCATTTCAAAACTC+GGG | + | chr8.1:59515237-59515256 | MS.gene99544:CDS | 30.0% |
| ! | ATTAACTTTAGTTCCATCAG+AGG | - | chr8.1:59515438-59515457 | None:intergenic | 30.0% |
| ! | GAATTTGCCATTTCAAAACT+CGG | + | chr8.1:59515236-59515255 | MS.gene99544:CDS | 30.0% |
| ! | GTTGAATATTGGAAAGCTTT+TGG | - | chr8.1:59515061-59515080 | None:intergenic | 30.0% |
| ! | TGAAGGCAAATTACTCTTTT+TGG | + | chr8.1:59514998-59515017 | MS.gene99544:CDS | 30.0% |
| AACTTACATGGTTCCATTGT+TGG | + | chr8.1:59515412-59515431 | MS.gene99544:CDS | 35.0% | |
| AAGTTGTTGATGCATTCACT+TGG | - | chr8.1:59515397-59515416 | None:intergenic | 35.0% | |
| ATTCAACCATTCCGAACAAT+AGG | + | chr8.1:59515074-59515093 | MS.gene99544:CDS | 35.0% | |
| GAATGCATCAACAACTTACA+TGG | + | chr8.1:59515400-59515419 | MS.gene99544:CDS | 35.0% | |
| GATTCATACCTCTTGTATCA+TGG | - | chr8.1:59515478-59515497 | None:intergenic | 35.0% | |
| TGTTCGGAATGGTTGAATAT+TGG | - | chr8.1:59515072-59515091 | None:intergenic | 35.0% | |
| ! | GGCAAATTCCTTCATTGATT+CGG | - | chr8.1:59515225-59515244 | None:intergenic | 35.0% |
| ! | TATAGTCTTGACGAGATTTG+TGG | + | chr8.1:59515155-59515174 | MS.gene99544:CDS | 35.0% |
| !! | AAATTCCTTCATTGATTCGG+AGG | - | chr8.1:59515222-59515241 | None:intergenic | 35.0% |
| AACATCCTCCGAATCAATGA+AGG | + | chr8.1:59515214-59515233 | MS.gene99544:CDS | 40.0% | |
| AACCATCCTATTGTTCGGAA+TGG | - | chr8.1:59515083-59515102 | None:intergenic | 40.0% | |
| AACCATTCCGAACAATAGGA+TGG | + | chr8.1:59515078-59515097 | MS.gene99544:CDS | 40.0% | |
| GATTCACTTGCAACAGGAAT+AGG | - | chr8.1:59515587-59515606 | None:intergenic | 40.0% | |
| TACCAACAAAATGGCAGACT+GGG | - | chr8.1:59515544-59515563 | None:intergenic | 40.0% | |
| TCATCAGATTCACTTGCAAC+AGG | - | chr8.1:59515593-59515612 | None:intergenic | 40.0% | |
| TTACCAACAAAATGGCAGAC+TGG | - | chr8.1:59515545-59515564 | None:intergenic | 40.0% | |
| TTCTCCACTACATTTCCAGA+CGG | - | chr8.1:59515128-59515147 | None:intergenic | 40.0% | |
| TTGGAAAATGACCTATACCC+TGG | + | chr8.1:59515017-59515036 | MS.gene99544:CDS | 40.0% | |
| TTGTTGATGCATTCACTTGG+TGG | - | chr8.1:59515394-59515413 | None:intergenic | 40.0% | |
| ! | TCATTTTCTTGCCAGGGTAT+AGG | - | chr8.1:59515031-59515050 | None:intergenic | 40.0% |
| !! | CAGCCTTGTTACCAACAAAA+TGG | - | chr8.1:59515553-59515572 | None:intergenic | 40.0% |
| !!! | CTGCCATTTTGTTGGTAACA+AGG | + | chr8.1:59515547-59515566 | MS.gene99544:CDS | 40.0% |
| !!! | GGTTGAGTAGCTTTTTCGAT+CGG | - | chr8.1:59515107-59515126 | None:intergenic | 40.0% |
| !!! | TTTCGTCCCGAGTTTTGAAA+TGG | - | chr8.1:59515246-59515265 | None:intergenic | 40.0% |
| AACTTGTCTTCTCCGATAGC+AGG | - | chr8.1:59515188-59515207 | None:intergenic | 45.0% | |
| AGTCTTGACGAGATTTGTGG+AGG | + | chr8.1:59515158-59515177 | MS.gene99544:CDS | 45.0% | |
| GAAAAAGCTACTCAACCGTC+TGG | + | chr8.1:59515110-59515129 | MS.gene99544:CDS | 45.0% | |
| GAAGTTCTCAAAGTCAAGCC+TGG | + | chr8.1:59515512-59515531 | MS.gene99544:CDS | 45.0% | |
| GCTTGTCACCATGATACAAG+AGG | + | chr8.1:59515467-59515486 | MS.gene99544:CDS | 45.0% | |
| TACCCTGGCAAGAAAATGAG+CGG | + | chr8.1:59515032-59515051 | MS.gene99544:CDS | 45.0% | |
| TCAACCGTCTGGAAATGTAG+TGG | + | chr8.1:59515121-59515140 | MS.gene99544:CDS | 45.0% | |
| TCGGCAACCATCCTATTGTT+CGG | - | chr8.1:59515088-59515107 | None:intergenic | 45.0% | |
| ! | TTGGTAACAAGGCTGTTGCT+TGG | + | chr8.1:59515558-59515577 | MS.gene99544:CDS | 45.0% |
| !!! | ATCCCAGTCTGCCATTTTGT+TGG | + | chr8.1:59515539-59515558 | MS.gene99544:CDS | 45.0% |
| GTTCCATCAGAGGCCAACAA+TGG | - | chr8.1:59515428-59515447 | None:intergenic | 50.0% | |
| TTGTCTTCTCCGATAGCAGG+TGG | - | chr8.1:59515185-59515204 | None:intergenic | 50.0% | |
| ! | AGACCGCTCATTTTCTTGCC+AGG | - | chr8.1:59515038-59515057 | None:intergenic | 50.0% |
| ! | GACCGCTCATTTTCTTGCCA+GGG | - | chr8.1:59515037-59515056 | None:intergenic | 50.0% |
| ! | GTTCCATTGTTGGCCTCTGA+TGG | + | chr8.1:59515422-59515441 | MS.gene99544:CDS | 50.0% |
| TGGCAGACTGGGATAGTTCC+AGG | - | chr8.1:59515533-59515552 | None:intergenic | 55.0% | |
| TGTGGAGGTCCACCTGCTAT+CGG | + | chr8.1:59515173-59515192 | MS.gene99544:CDS | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr8.1 | gene | 59514975 | 59515631 | 59514975 | ID=MS.gene99544 |
| chr8.1 | mRNA | 59514975 | 59515631 | 59514975 | ID=MS.gene99544.t1;Parent=MS.gene99544 |
| chr8.1 | exon | 59514975 | 59515631 | 59514975 | ID=MS.gene99544.t1.exon1;Parent=MS.gene99544.t1 |
| chr8.1 | CDS | 59514975 | 59515631 | 59514975 | ID=cds.MS.gene99544.t1;Parent=MS.gene99544.t1 |
| Gene Sequence |
| Protein sequence |
