Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene72924.t1 | XP_003608741.1 | 100 | 128 | 0 | 0 | 1 | 128 | 5 | 132 | 4.90E-57 | 230.3 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene72924.t1 | P14009 | 64.8 | 128 | 44 | 1 | 1 | 127 | 9 | 136 | 1.2e-30 | 134.0 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene72924.t1 | G7JIZ5 | 100.0 | 128 | 0 | 0 | 1 | 128 | 5 | 132 | 3.5e-57 | 230.3 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049134 | MS.gene72924 | 0.800338 | 1.51E-48 | -1.69E-46 |
| MS.gene049149 | MS.gene72924 | -0.825626 | 4.23E-54 | -1.69E-46 |
| MS.gene049150 | MS.gene72924 | -0.82067 | 6.07E-53 | -1.69E-46 |
| MS.gene049153 | MS.gene72924 | -0.828878 | 7.04E-55 | -1.69E-46 |
| MS.gene04933 | MS.gene72924 | -0.810039 | 1.41E-50 | -1.69E-46 |
| MS.gene049758 | MS.gene72924 | -0.806006 | 1.01E-49 | -1.69E-46 |
| MS.gene049993 | MS.gene72924 | -0.899579 | 1.72E-77 | -1.69E-46 |
| MS.gene050133 | MS.gene72924 | 0.812423 | 4.27E-51 | -1.69E-46 |
| MS.gene050301 | MS.gene72924 | -0.806936 | 6.46E-50 | -1.69E-46 |
| MS.gene050397 | MS.gene72924 | -0.839246 | 1.78E-57 | -1.69E-46 |
| MS.gene050406 | MS.gene72924 | -0.806158 | 9.42E-50 | -1.69E-46 |
| MS.gene050426 | MS.gene72924 | 0.854142 | 1.50E-61 | -1.69E-46 |
| MS.gene050464 | MS.gene72924 | -0.874152 | 8.44E-68 | -1.69E-46 |
| MS.gene050529 | MS.gene72924 | -0.841654 | 4.17E-58 | -1.69E-46 |
| MS.gene050623 | MS.gene72924 | 0.802741 | 4.86E-49 | -1.69E-46 |
| MS.gene050642 | MS.gene72924 | -0.851418 | 8.99E-61 | -1.69E-46 |
| MS.gene050670 | MS.gene72924 | -0.807844 | 4.14E-50 | -1.69E-46 |
| MS.gene050971 | MS.gene72924 | -0.811586 | 6.50E-51 | -1.69E-46 |
| MS.gene051158 | MS.gene72924 | 0.836126 | 1.12E-56 | -1.69E-46 |
| MS.gene051525 | MS.gene72924 | -0.833354 | 5.60E-56 | -1.69E-46 |
| MS.gene051860 | MS.gene72924 | -0.869754 | 2.44E-66 | -1.69E-46 |
| MS.gene051983 | MS.gene72924 | -0.859838 | 3.14E-63 | -1.69E-46 |
| MS.gene052011 | MS.gene72924 | 0.90133 | 2.99E-78 | -1.69E-46 |
| MS.gene052077 | MS.gene72924 | -0.851102 | 1.10E-60 | -1.69E-46 |
| MS.gene052128 | MS.gene72924 | 0.830328 | 3.12E-55 | -1.69E-46 |
| MS.gene052134 | MS.gene72924 | -0.809731 | 1.64E-50 | -1.69E-46 |
| MS.gene052135 | MS.gene72924 | -0.813059 | 3.10E-51 | -1.69E-46 |
| MS.gene052188 | MS.gene72924 | -0.80163 | 8.22E-49 | -1.69E-46 |
| MS.gene052223 | MS.gene72924 | 0.849364 | 3.39E-60 | -1.69E-46 |
| MS.gene052253 | MS.gene72924 | -0.847959 | 8.31E-60 | -1.69E-46 |
| MS.gene05233 | MS.gene72924 | 0.84295 | 1.89E-58 | -1.69E-46 |
| MS.gene052340 | MS.gene72924 | -0.818202 | 2.22E-52 | -1.69E-46 |
| MS.gene052758 | MS.gene72924 | -0.803864 | 2.84E-49 | -1.69E-46 |
| MS.gene053330 | MS.gene72924 | -0.817445 | 3.29E-52 | -1.69E-46 |
| MS.gene053557 | MS.gene72924 | -0.80753 | 4.83E-50 | -1.69E-46 |
| MS.gene053836 | MS.gene72924 | -0.855855 | 4.77E-62 | -1.69E-46 |
| MS.gene053971 | MS.gene72924 | -0.812439 | 4.24E-51 | -1.69E-46 |
| MS.gene054011 | MS.gene72924 | -0.808633 | 2.81E-50 | -1.69E-46 |
| MS.gene054012 | MS.gene72924 | -0.804019 | 2.64E-49 | -1.69E-46 |
| MS.gene054102 | MS.gene72924 | -0.805871 | 1.08E-49 | -1.69E-46 |
| MS.gene054173 | MS.gene72924 | -0.801226 | 9.95E-49 | -1.69E-46 |
| MS.gene054183 | MS.gene72924 | -0.899116 | 2.72E-77 | -1.69E-46 |
| MS.gene054349 | MS.gene72924 | -0.845053 | 5.16E-59 | -1.69E-46 |
| MS.gene054473 | MS.gene72924 | -0.811863 | 5.66E-51 | -1.69E-46 |
| MS.gene054719 | MS.gene72924 | -0.836826 | 7.45E-57 | -1.69E-46 |
| MS.gene054918 | MS.gene72924 | -0.835447 | 1.67E-56 | -1.69E-46 |
| MS.gene054983 | MS.gene72924 | -0.83982 | 1.26E-57 | -1.69E-46 |
| MS.gene055116 | MS.gene72924 | -0.804902 | 1.73E-49 | -1.69E-46 |
| MS.gene055157 | MS.gene72924 | 0.821399 | 4.12E-53 | -1.69E-46 |
| MS.gene055217 | MS.gene72924 | -0.804828 | 1.79E-49 | -1.69E-46 |
| MS.gene055277 | MS.gene72924 | -0.806288 | 8.85E-50 | -1.69E-46 |
| MS.gene055358 | MS.gene72924 | -0.823358 | 1.45E-53 | -1.69E-46 |
| MS.gene055369 | MS.gene72924 | -0.80079 | 1.22E-48 | -1.69E-46 |
| MS.gene055422 | MS.gene72924 | -0.811928 | 5.48E-51 | -1.69E-46 |
| MS.gene05553 | MS.gene72924 | -0.840899 | 6.58E-58 | -1.69E-46 |
| MS.gene055595 | MS.gene72924 | 0.802912 | 4.48E-49 | -1.69E-46 |
| MS.gene055597 | MS.gene72924 | 0.800674 | 1.29E-48 | -1.69E-46 |
| MS.gene055601 | MS.gene72924 | 0.874134 | 8.56E-68 | -1.69E-46 |
| MS.gene055604 | MS.gene72924 | -0.813465 | 2.53E-51 | -1.69E-46 |
| MS.gene056233 | MS.gene72924 | -0.812418 | 4.28E-51 | -1.69E-46 |
| MS.gene056330 | MS.gene72924 | 0.836241 | 1.05E-56 | -1.69E-46 |
| MS.gene056337 | MS.gene72924 | -0.800502 | 1.40E-48 | -1.69E-46 |
| MS.gene056612 | MS.gene72924 | -0.831157 | 1.96E-55 | -1.69E-46 |
| MS.gene056659 | MS.gene72924 | -0.813216 | 2.86E-51 | -1.69E-46 |
| MS.gene056690 | MS.gene72924 | 0.816799 | 4.59E-52 | -1.69E-46 |
| MS.gene056691 | MS.gene72924 | 0.812316 | 4.51E-51 | -1.69E-46 |
| MS.gene056723 | MS.gene72924 | -0.82289 | 1.86E-53 | -1.69E-46 |
| MS.gene056899 | MS.gene72924 | 0.907377 | 5.41E-81 | -1.69E-46 |
| MS.gene056924 | MS.gene72924 | 0.800322 | 1.52E-48 | -1.69E-46 |
| MS.gene056979 | MS.gene72924 | -0.855811 | 4.91E-62 | -1.69E-46 |
| MS.gene05702 | MS.gene72924 | -0.820196 | 7.79E-53 | -1.69E-46 |
| MS.gene057121 | MS.gene72924 | -0.810957 | 8.90E-51 | -1.69E-46 |
| MS.gene057245 | MS.gene72924 | -0.813085 | 3.06E-51 | -1.69E-46 |
| MS.gene057396 | MS.gene72924 | -0.804045 | 2.61E-49 | -1.69E-46 |
| MS.gene057476 | MS.gene72924 | -0.841975 | 3.43E-58 | -1.69E-46 |
| MS.gene057749 | MS.gene72924 | -0.821866 | 3.21E-53 | -1.69E-46 |
| MS.gene057823 | MS.gene72924 | -0.813185 | 2.91E-51 | -1.69E-46 |
| MS.gene057888 | MS.gene72924 | -0.80624 | 9.06E-50 | -1.69E-46 |
| MS.gene058002 | MS.gene72924 | -0.832314 | 1.01E-55 | -1.69E-46 |
| MS.gene05811 | MS.gene72924 | -0.825825 | 3.79E-54 | -1.69E-46 |
| MS.gene058142 | MS.gene72924 | -0.84632 | 2.34E-59 | -1.69E-46 |
| MS.gene058218 | MS.gene72924 | 0.865416 | 6.00E-65 | -1.69E-46 |
| MS.gene05856 | MS.gene72924 | -0.804924 | 1.71E-49 | -1.69E-46 |
| MS.gene058710 | MS.gene72924 | -0.82022 | 7.70E-53 | -1.69E-46 |
| MS.gene05886 | MS.gene72924 | -0.827232 | 1.75E-54 | -1.69E-46 |
| MS.gene058928 | MS.gene72924 | -0.805529 | 1.28E-49 | -1.69E-46 |
| MS.gene059011 | MS.gene72924 | -0.814435 | 1.54E-51 | -1.69E-46 |
| MS.gene059043 | MS.gene72924 | 0.847147 | 1.39E-59 | -1.69E-46 |
| MS.gene05907 | MS.gene72924 | -0.846288 | 2.39E-59 | -1.69E-46 |
| MS.gene059247 | MS.gene72924 | -0.804142 | 2.49E-49 | -1.69E-46 |
| MS.gene059285 | MS.gene72924 | -0.820882 | 5.42E-53 | -1.69E-46 |
| MS.gene059294 | MS.gene72924 | -0.824631 | 7.27E-54 | -1.69E-46 |
| MS.gene059420 | MS.gene72924 | -0.876488 | 1.34E-68 | -1.69E-46 |
| MS.gene059462 | MS.gene72924 | -0.800134 | 1.66E-48 | -1.69E-46 |
| MS.gene059609 | MS.gene72924 | -0.830808 | 2.38E-55 | -1.69E-46 |
| MS.gene05984 | MS.gene72924 | -0.852107 | 5.73E-61 | -1.69E-46 |
| MS.gene059894 | MS.gene72924 | -0.805493 | 1.30E-49 | -1.69E-46 |
| MS.gene060038 | MS.gene72924 | -0.804338 | 2.27E-49 | -1.69E-46 |
| MS.gene060247 | MS.gene72924 | -0.805156 | 1.53E-49 | -1.69E-46 |
| MS.gene061049 | MS.gene72924 | -0.856751 | 2.60E-62 | -1.69E-46 |
| MS.gene061114 | MS.gene72924 | 0.864935 | 8.50E-65 | -1.69E-46 |
| MS.gene061154 | MS.gene72924 | -0.816852 | 4.47E-52 | -1.69E-46 |
| MS.gene061159 | MS.gene72924 | -0.823583 | 1.28E-53 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene72924.t1 | MTR_4g101260 | 100.000 | 128 | 0 | 0 | 1 | 128 | 5 | 132 | 1.16e-88 | 253 |
| MS.gene72924.t1 | MTR_4g108150 | 89.844 | 128 | 4 | 1 | 1 | 128 | 5 | 123 | 5.31e-76 | 221 |
| MS.gene72924.t1 | MTR_2g026760 | 57.812 | 128 | 47 | 3 | 1 | 128 | 7 | 127 | 1.82e-39 | 129 |
| MS.gene72924.t1 | MTR_4g101280 | 54.815 | 135 | 54 | 2 | 1 | 128 | 9 | 143 | 8.84e-37 | 122 |
| MS.gene72924.t1 | MTR_1g012690 | 61.856 | 97 | 35 | 1 | 32 | 128 | 60 | 154 | 2.85e-36 | 121 |
| MS.gene72924.t1 | MTR_1g012700 | 55.833 | 120 | 40 | 2 | 22 | 128 | 45 | 164 | 4.14e-36 | 121 |
| MS.gene72924.t1 | MTR_4g101330 | 46.951 | 164 | 46 | 4 | 1 | 127 | 9 | 168 | 7.69e-36 | 121 |
| MS.gene72924.t1 | MTR_1g012710 | 65.060 | 83 | 29 | 0 | 46 | 128 | 69 | 151 | 2.74e-34 | 116 |
| MS.gene72924.t1 | MTR_1g012630 | 67.470 | 83 | 27 | 0 | 46 | 128 | 128 | 210 | 5.48e-34 | 117 |
| MS.gene72924.t1 | MTR_3g106740 | 69.880 | 83 | 24 | 1 | 46 | 127 | 47 | 129 | 6.74e-34 | 115 |
| MS.gene72924.t1 | MTR_4g101310 | 47.967 | 123 | 60 | 3 | 7 | 128 | 15 | 134 | 1.17e-28 | 102 |
| MS.gene72924.t1 | MTR_3g106750 | 61.905 | 84 | 31 | 1 | 46 | 128 | 46 | 129 | 2.46e-24 | 90.9 |
| MS.gene72924.t1 | MTR_5g012340 | 47.619 | 84 | 42 | 2 | 45 | 127 | 94 | 176 | 2.22e-23 | 89.7 |
| MS.gene72924.t1 | MTR_5g012380 | 47.619 | 84 | 42 | 2 | 45 | 127 | 87 | 169 | 6.08e-23 | 88.6 |
| MS.gene72924.t1 | MTR_4g101270 | 54.762 | 84 | 36 | 2 | 45 | 127 | 104 | 186 | 2.96e-21 | 84.3 |
| MS.gene72924.t1 | MTR_2g026930 | 45.570 | 79 | 41 | 1 | 49 | 127 | 122 | 198 | 5.46e-17 | 73.6 |
| MS.gene72924.t1 | MTR_2g026920 | 45.570 | 79 | 41 | 1 | 49 | 127 | 118 | 194 | 6.55e-17 | 73.2 |
| MS.gene72924.t1 | MTR_2g026775 | 46.053 | 76 | 36 | 2 | 55 | 128 | 688 | 760 | 7.19e-16 | 72.8 |
| MS.gene72924.t1 | MTR_2g026820 | 46.154 | 78 | 37 | 3 | 51 | 127 | 542 | 615 | 8.96e-13 | 63.9 |
| MS.gene72924.t1 | MTR_3g082100 | 42.857 | 84 | 46 | 2 | 45 | 127 | 253 | 335 | 1.44e-12 | 63.2 |
| MS.gene72924.t1 | MTR_4g115360 | 48.235 | 85 | 42 | 2 | 45 | 128 | 150 | 233 | 5.43e-11 | 58.2 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene72924.t1 | AT2G45180 | 62.205 | 127 | 46 | 1 | 2 | 128 | 10 | 134 | 1.42e-45 | 144 |
| MS.gene72924.t1 | AT5G46890 | 63.281 | 128 | 41 | 4 | 1 | 128 | 6 | 127 | 9.47e-40 | 129 |
| MS.gene72924.t1 | AT4G12520 | 51.163 | 129 | 54 | 2 | 1 | 128 | 9 | 129 | 6.20e-37 | 122 |
| MS.gene72924.t1 | AT4G12510 | 51.163 | 129 | 54 | 2 | 1 | 128 | 9 | 129 | 6.20e-37 | 122 |
| MS.gene72924.t1 | AT5G46900 | 61.719 | 128 | 43 | 3 | 1 | 128 | 6 | 127 | 1.07e-36 | 122 |
| MS.gene72924.t1 | AT1G62510 | 72.289 | 83 | 22 | 1 | 46 | 127 | 67 | 149 | 3.04e-36 | 121 |
| MS.gene72924.t1 | AT4G00165 | 50.806 | 124 | 45 | 3 | 8 | 127 | 17 | 128 | 1.28e-34 | 117 |
| MS.gene72924.t1 | AT4G00165 | 50.806 | 124 | 45 | 3 | 8 | 127 | 17 | 128 | 1.28e-34 | 117 |
| MS.gene72924.t1 | AT4G12480 | 40.625 | 160 | 63 | 2 | 1 | 128 | 9 | 168 | 9.92e-34 | 115 |
| MS.gene72924.t1 | AT1G12090 | 71.605 | 81 | 22 | 1 | 48 | 127 | 57 | 137 | 2.78e-33 | 113 |
| MS.gene72924.t1 | AT4G22460 | 58.095 | 105 | 40 | 3 | 22 | 125 | 29 | 130 | 3.47e-33 | 113 |
| MS.gene72924.t1 | AT4G12550 | 47.287 | 129 | 43 | 2 | 1 | 128 | 7 | 111 | 4.25e-33 | 112 |
| MS.gene72924.t1 | AT4G12490 | 62.105 | 95 | 33 | 2 | 35 | 128 | 90 | 182 | 1.95e-32 | 112 |
| MS.gene72924.t1 | AT4G12500 | 63.095 | 84 | 30 | 1 | 46 | 128 | 94 | 177 | 1.02e-31 | 110 |
| MS.gene72924.t1 | AT4G12470 | 58.427 | 89 | 36 | 1 | 41 | 128 | 73 | 161 | 1.53e-31 | 110 |
| MS.gene72924.t1 | AT4G12545 | 47.287 | 129 | 40 | 2 | 1 | 128 | 7 | 108 | 2.34e-31 | 107 |
| MS.gene72924.t1 | AT1G12100 | 45.312 | 128 | 64 | 3 | 2 | 128 | 10 | 132 | 5.52e-30 | 105 |
| MS.gene72924.t1 | AT4G12530 | 53.409 | 88 | 41 | 0 | 40 | 127 | 28 | 115 | 1.96e-27 | 98.2 |
| MS.gene72924.t1 | AT3G22142 | 52.381 | 84 | 38 | 2 | 45 | 127 | 1396 | 1478 | 4.19e-19 | 82.4 |
| MS.gene72924.t1 | AT2G10940 | 49.412 | 85 | 41 | 2 | 45 | 128 | 207 | 290 | 7.44e-19 | 80.1 |
| MS.gene72924.t1 | AT2G10940 | 49.412 | 85 | 41 | 2 | 45 | 128 | 207 | 290 | 7.44e-19 | 80.1 |
| MS.gene72924.t1 | AT1G62500 | 47.126 | 87 | 44 | 2 | 42 | 127 | 208 | 293 | 4.63e-18 | 78.2 |
| MS.gene72924.t1 | AT3G22120 | 54.118 | 85 | 36 | 3 | 45 | 127 | 266 | 349 | 5.84e-18 | 78.2 |
| MS.gene72924.t1 | AT3G22120 | 54.118 | 85 | 36 | 3 | 45 | 127 | 249 | 332 | 1.11e-17 | 77.4 |
| MS.gene72924.t1 | AT4G15160 | 51.163 | 86 | 38 | 4 | 45 | 127 | 98 | 182 | 4.16e-17 | 73.9 |
| MS.gene72924.t1 | AT4G15160 | 51.163 | 86 | 38 | 4 | 45 | 127 | 180 | 264 | 1.38e-16 | 73.9 |
| MS.gene72924.t1 | AT4G22490 | 35.165 | 91 | 53 | 2 | 39 | 127 | 30 | 116 | 3.45e-12 | 59.3 |
| MS.gene72924.t1 | AT4G22520 | 27.559 | 127 | 72 | 3 | 1 | 127 | 11 | 117 | 4.97e-11 | 56.2 |
Find 37 sgRNAs with CRISPR-Local
Find 45 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AAGTACCAAGCTCAAGTTAA+TGG | 0.256664 | 8.3:-19959726 | None:intergenic |
| TCAAGTTAATGGGAAGATTA+AGG | 0.276650 | 8.3:-19959715 | None:intergenic |
| TGGTGTGTGTGCTGATGTAT+TGG | 0.312025 | 8.3:+19959565 | MS.gene72924:CDS |
| GTGCCTTTGCACTGCTCTTA+AGG | 0.336313 | 8.3:+19959673 | MS.gene72924:CDS |
| GGTGTGTGTGCTGATGTATT+GGG | 0.364523 | 8.3:+19959566 | MS.gene72924:CDS |
| CCATGCTGCTCTCTCATTGA+TGG | 0.378656 | 8.3:+19959626 | MS.gene72924:CDS |
| AGAGAGCAGCATGGTGTCTT+TGG | 0.413985 | 8.3:-19959617 | None:intergenic |
| CCTAGAGACACAATCAAGTT+TGG | 0.422527 | 8.3:+19959545 | MS.gene72924:CDS |
| TAAGCGCACACGAAACCCTT+TGG | 0.426755 | 8.3:-19959770 | None:intergenic |
| AGTACCAAGCTCAAGTTAAT+GGG | 0.431914 | 8.3:-19959725 | None:intergenic |
| TTGTGTCTCTAGGGCATGTT+GGG | 0.436629 | 8.3:-19959535 | None:intergenic |
| CGTGATCTTTGTGAGGTGGT+GGG | 0.442572 | 8.3:-19959475 | None:intergenic |
| CCGTGATCTTTGTGAGGTGG+TGG | 0.443706 | 8.3:-19959476 | None:intergenic |
| GTACTTAACTATTGTGGAAA+GGG | 0.444010 | 8.3:+19959743 | MS.gene72924:CDS |
| GAGCAGCATGGTGTCTTTGG+TGG | 0.477521 | 8.3:-19959614 | None:intergenic |
| ATTGTGTCTCTAGGGCATGT+TGG | 0.477867 | 8.3:-19959536 | None:intergenic |
| CCAAACTTGATTGTGTCTCT+AGG | 0.479073 | 8.3:-19959545 | None:intergenic |
| GTGATCTTTGTGAGGTGGTG+GGG | 0.487931 | 8.3:-19959474 | None:intergenic |
| GCTCTTAAGGCTAATGTCCT+AGG | 0.488278 | 8.3:+19959686 | MS.gene72924:CDS |
| TGGAGGAAGGTGGATGATGA+GGG | 0.490113 | 8.3:-19959508 | None:intergenic |
| TTGGAGGAAGGTGGATGATG+AGG | 0.490518 | 8.3:-19959509 | None:intergenic |
| TCTTCCCATTAACTTGAGCT+TGG | 0.493017 | 8.3:+19959721 | MS.gene72924:CDS |
| AGCTTGGTACTTAACTATTG+TGG | 0.494742 | 8.3:+19959737 | MS.gene72924:CDS |
| GGTTTGATTAATGTTGAGCT+TGG | 0.521860 | 8.3:+19959587 | MS.gene72924:CDS |
| TGATCTTTGTGAGGTGGTGG+GGG | 0.527911 | 8.3:-19959473 | None:intergenic |
| GGTACTTAACTATTGTGGAA+AGG | 0.533566 | 8.3:+19959742 | MS.gene72924:CDS |
| AAAATATGATGTTGAGGCAA+AGG | 0.535029 | 8.3:-19959418 | None:intergenic |
| GAAGATTAAGGTTAATGCCT+AGG | 0.539426 | 8.3:-19959703 | None:intergenic |
| CAAACTTGATTGTGTCTCTA+GGG | 0.541313 | 8.3:-19959544 | None:intergenic |
| TTGTGGAAAGGGAGTTCCAA+AGG | 0.553018 | 8.3:+19959754 | MS.gene72924:CDS |
| TGTGGAAAGGGAGTTCCAAA+GGG | 0.568343 | 8.3:+19959755 | MS.gene72924:CDS |
| CCACCACCTCACAAAGATCA+CGG | 0.588604 | 8.3:+19959476 | MS.gene72924:CDS |
| GTGGATGATGAGGGTGTGAG+TGG | 0.631843 | 8.3:-19959499 | None:intergenic |
| TAGCCTTAAGAGCAGTGCAA+AGG | 0.634750 | 8.3:-19959676 | None:intergenic |
| TGGCCGTGATCTTTGTGAGG+TGG | 0.666290 | 8.3:-19959479 | None:intergenic |
| CCATCAATGAGAGAGCAGCA+TGG | 0.704306 | 8.3:-19959626 | None:intergenic |
| GAGTGGCCGTGATCTTTGTG+AGG | 0.710036 | 8.3:-19959482 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| AAAATATGATGTTGAGGCAA+AGG | - | chr8.3:19959421-19959440 | None:intergenic | 30.0% | |
| CAGTGAAAAATATGATGTTG+AGG | - | chr8.3:19959427-19959446 | None:intergenic | 30.0% | |
| GTACTTAACTATTGTGGAAA+GGG | + | chr8.3:19959743-19959762 | MS.gene72924:CDS | 30.0% | |
| TCAAGTTAATGGGAAGATTA+AGG | - | chr8.3:19959718-19959737 | None:intergenic | 30.0% | |
| ! | CAACATCATATTTTTCACTG+TGG | + | chr8.3:19959427-19959446 | MS.gene72924:CDS | 30.0% |
| AAGTACCAAGCTCAAGTTAA+TGG | - | chr8.3:19959729-19959748 | None:intergenic | 35.0% | |
| AGCTTGGTACTTAACTATTG+TGG | + | chr8.3:19959737-19959756 | MS.gene72924:CDS | 35.0% | |
| AGTACCAAGCTCAAGTTAAT+GGG | - | chr8.3:19959728-19959747 | None:intergenic | 35.0% | |
| CAAACTTGATTGTGTCTCTA+GGG | - | chr8.3:19959547-19959566 | None:intergenic | 35.0% | |
| GAAGATTAAGGTTAATGCCT+AGG | - | chr8.3:19959706-19959725 | None:intergenic | 35.0% | |
| GGTACTTAACTATTGTGGAA+AGG | + | chr8.3:19959742-19959761 | MS.gene72924:CDS | 35.0% | |
| GGTTTGATTAATGTTGAGCT+TGG | + | chr8.3:19959587-19959606 | MS.gene72924:CDS | 35.0% | |
| CCAAACTTGATTGTGTCTCT+AGG | - | chr8.3:19959548-19959567 | None:intergenic | 40.0% | |
| CCTAGAGACACAATCAAGTT+TGG | + | chr8.3:19959545-19959564 | MS.gene72924:CDS | 40.0% | |
| ! | TCTTCCCATTAACTTGAGCT+TGG | + | chr8.3:19959721-19959740 | MS.gene72924:CDS | 40.0% |
| GCTCTTAAGGCTAATGTCCT+AGG | + | chr8.3:19959686-19959705 | MS.gene72924:CDS | 45.0% | |
| TGTGGAAAGGGAGTTCCAAA+GGG | + | chr8.3:19959755-19959774 | MS.gene72924:CDS | 45.0% | |
| TTGTGGAAAGGGAGTTCCAA+AGG | + | chr8.3:19959754-19959773 | MS.gene72924:CDS | 45.0% | |
| ! | GGTGTGTGTGCTGATGTATT+GGG | + | chr8.3:19959566-19959585 | MS.gene72924:CDS | 45.0% |
| ! | TAGCCTTAAGAGCAGTGCAA+AGG | - | chr8.3:19959679-19959698 | None:intergenic | 45.0% |
| ! | TGGTGTGTGTGCTGATGTAT+TGG | + | chr8.3:19959565-19959584 | MS.gene72924:CDS | 45.0% |
| !! | ATTGTGTCTCTAGGGCATGT+TGG | - | chr8.3:19959539-19959558 | None:intergenic | 45.0% |
| !! | CATGTTGGGTTTTTGGAGGA+AGG | - | chr8.3:19959524-19959543 | None:intergenic | 45.0% |
| !! | TCTAGGGCATGTTGGGTTTT+TGG | - | chr8.3:19959531-19959550 | None:intergenic | 45.0% |
| !! | TTGTGTCTCTAGGGCATGTT+GGG | - | chr8.3:19959538-19959557 | None:intergenic | 45.0% |
| AGAGAGCAGCATGGTGTCTT+TGG | - | chr8.3:19959620-19959639 | None:intergenic | 50.0% | |
| CCACCACCTCACAAAGATCA+CGG | + | chr8.3:19959476-19959495 | MS.gene72924:CDS | 50.0% | |
| CCATCAATGAGAGAGCAGCA+TGG | - | chr8.3:19959629-19959648 | None:intergenic | 50.0% | |
| CCATGCTGCTCTCTCATTGA+TGG | + | chr8.3:19959626-19959645 | MS.gene72924:CDS | 50.0% | |
| CGTGATCTTTGTGAGGTGGT+GGG | - | chr8.3:19959478-19959497 | None:intergenic | 50.0% | |
| GTGATCTTTGTGAGGTGGTG+GGG | - | chr8.3:19959477-19959496 | None:intergenic | 50.0% | |
| GTGCCTTTGCACTGCTCTTA+AGG | + | chr8.3:19959673-19959692 | MS.gene72924:CDS | 50.0% | |
| TAAGCGCACACGAAACCCTT+TGG | - | chr8.3:19959773-19959792 | None:intergenic | 50.0% | |
| TGGAGGAAGGTGGATGATGA+GGG | - | chr8.3:19959511-19959530 | None:intergenic | 50.0% | |
| TGGGGGACATGGAACATATG+TGG | - | chr8.3:19959459-19959478 | None:intergenic | 50.0% | |
| TTGGAGGAAGGTGGATGATG+AGG | - | chr8.3:19959512-19959531 | None:intergenic | 50.0% | |
| ! | TGATCTTTGTGAGGTGGTGG+GGG | - | chr8.3:19959476-19959495 | None:intergenic | 50.0% |
| !! | AGGGCATGTTGGGTTTTTGG+AGG | - | chr8.3:19959528-19959547 | None:intergenic | 50.0% |
| !! | GTTGGGTTTTTGGAGGAAGG+TGG | - | chr8.3:19959521-19959540 | None:intergenic | 50.0% |
| CCGTGATCTTTGTGAGGTGG+TGG | - | chr8.3:19959479-19959498 | None:intergenic | 55.0% | |
| GAGCAGCATGGTGTCTTTGG+TGG | - | chr8.3:19959617-19959636 | None:intergenic | 55.0% | |
| GAGTGGCCGTGATCTTTGTG+AGG | - | chr8.3:19959485-19959504 | None:intergenic | 55.0% | |
| TGGCCGTGATCTTTGTGAGG+TGG | - | chr8.3:19959482-19959501 | None:intergenic | 55.0% | |
| ! | GTGGATGATGAGGGTGTGAG+TGG | - | chr8.3:19959502-19959521 | None:intergenic | 55.0% |
| !! | TTGTGAGGTGGTGGGGGACA+TGG | - | chr8.3:19959470-19959489 | None:intergenic | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr8.3 | gene | 19959407 | 19959793 | 19959407 | ID=MS.gene72924 |
| chr8.3 | mRNA | 19959407 | 19959793 | 19959407 | ID=MS.gene72924.t1;Parent=MS.gene72924 |
| chr8.3 | exon | 19959407 | 19959793 | 19959407 | ID=MS.gene72924.t1.exon1;Parent=MS.gene72924.t1 |
| chr8.3 | CDS | 19959407 | 19959793 | 19959407 | ID=cds.MS.gene72924.t1;Parent=MS.gene72924.t1 |
| Gene Sequence |
| Protein sequence |
