AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene35690


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene35690.t1 MTR_2g086740 96.818 220 7 0 1 220 1 220 2.13e-157 434
MS.gene35690.t1 MTR_2g086740 96.667 150 5 0 1 150 1 150 8.28e-106 301
MS.gene35690.t1 MTR_1g058540 59.064 171 70 0 49 219 25 195 4.12e-61 190
MS.gene35690.t1 MTR_1g058560 59.538 173 70 0 47 219 22 194 2.04e-57 180
MS.gene35690.t1 MTR_1g058570 56.725 171 74 0 49 219 23 193 3.29e-55 175
MS.gene35690.t1 MTR_7g011090 48.276 174 90 0 46 219 12 185 1.41e-50 162
MS.gene35690.t1 MTR_4g081880 43.243 185 99 1 42 220 3 187 1.08e-46 153
MS.gene35690.t1 MTR_5g041900 43.590 195 100 1 26 220 2 186 4.12e-42 141
MS.gene35690.t1 MTR_7g083120 33.094 139 90 2 65 202 33 169 3.80e-16 73.9
MS.gene35690.t1 MTR_0004s0210 34.899 149 84 4 55 201 1 138 6.16e-16 72.8
MS.gene35690.t1 MTR_7g108130 28.877 187 115 5 24 206 3 175 4.40e-15 71.2
MS.gene35690.t1 MTR_3g064310 28.141 199 103 5 40 216 7 187 3.36e-14 69.3
MS.gene35690.t1 MTR_0097s0070 29.565 115 81 0 99 213 71 185 4.90e-12 63.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene35690.t1 AT2G27370 49.091 220 103 4 3 220 8 220 3.56e-64 198
MS.gene35690.t1 AT2G36100 54.762 168 76 0 52 219 38 205 9.44e-59 184
MS.gene35690.t1 AT5G06200 60.479 167 66 0 53 219 35 201 3.92e-54 172
MS.gene35690.t1 AT5G15290 49.686 159 79 1 43 200 8 166 1.53e-51 165
MS.gene35690.t1 AT3G11550 59.880 167 67 0 53 219 37 203 6.86e-50 161
MS.gene35690.t1 AT1G14160 43.030 165 94 0 55 219 44 208 2.74e-42 142
MS.gene35690.t1 AT1G17200 29.193 161 105 3 58 217 29 181 2.77e-15 72.0
MS.gene35690.t1 AT3G06390 28.161 174 104 4 24 190 11 170 3.28e-15 71.6
MS.gene35690.t1 AT5G44550 30.247 162 101 4 66 219 22 179 5.97e-12 62.8
MS.gene35690.t1 AT1G03700 32.000 150 80 5 65 206 12 147 2.96e-11 60.1
MS.gene35690.t1 AT4G15610 27.545 167 117 2 40 202 7 173 5.35e-11 60.1

Find 48 sgRNAs with CRISPR-Local

Find 89 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTCTTGTTCTCTTGGATTCT+AGG 0.131201 2.4:+13645133 None:intergenic
TCTTGGTGCTTCTGTCATTA+TGG 0.177988 2.4:-13644956 MS.gene35690:CDS
TACCTAATTGCAGGTATTAA+TGG 0.240857 2.4:-13644049 MS.gene35690:intron
TCCATTGCTTCTTGTTCTCT+TGG 0.247900 2.4:+13645125 None:intergenic
CTTGGTGCTTCTGTCATTAT+GGG 0.276810 2.4:-13644955 MS.gene35690:CDS
AAATCTACTCCAATCCAAAA+AGG 0.350579 2.4:-13645042 MS.gene35690:CDS
TCCTTCAGTTTCATGCTCAA+TGG 0.391797 2.4:-13644897 MS.gene35690:CDS
CCTTCAGTTTCATGCTCAAT+GGG 0.394063 2.4:-13644896 MS.gene35690:CDS
GGTGCCATTGGTGCTACTCT+TGG 0.400369 2.4:-13644973 MS.gene35690:CDS
TGTTCTCTTGGATTCTAGGA+TGG 0.406134 2.4:+13645137 None:intergenic
GCAGGTATTAATGGCCTTAG+TGG 0.406928 2.4:-13644040 MS.gene35690:intron
ACATGAACTTACTTAAACAT+TGG 0.425523 2.4:+13644865 None:intergenic
AGAACAAGAAGCAATGGAAA+AGG 0.427010 2.4:-13645120 MS.gene35690:CDS
ACACCACAACCAAACAAGAT+AGG 0.440488 2.4:+13643869 None:intergenic
ATGGGAGTCAAGATGCAAAC+TGG 0.445257 2.4:-13643972 MS.gene35690:CDS
GTTCTCTTGGATTCTAGGAT+GGG 0.446748 2.4:+13645138 None:intergenic
CTAAACATGCAGGTTCTTTG+TGG 0.448810 2.4:-13644293 MS.gene35690:intron
GAACAAGAAGCAATGGAAAA+GGG 0.449907 2.4:-13645119 MS.gene35690:CDS
ATTGTTAGACCCCTTGCAGC+TGG 0.465698 2.4:-13644211 MS.gene35690:CDS
GGAAAAGGGAAATCCATTGA+TGG 0.488181 2.4:-13645105 MS.gene35690:CDS
TTTCCTATCTTGTTTGGTTG+TGG 0.489463 2.4:-13643872 MS.gene35690:CDS
CCAAGGTTCAAGTTTGGCTG+TGG 0.491266 2.4:-13643917 MS.gene35690:CDS
GTGGCAAATGGAGCAGCAGC+TGG 0.512854 2.4:-13644271 MS.gene35690:CDS
TTGTGTACTTAGCACACAAT+GGG 0.520027 2.4:-13643990 MS.gene35690:CDS
TGTCTTCTGTAGCTCTCAAA+AGG 0.525875 2.4:-13643849 MS.gene35690:CDS
AGGTTCTTTGTGGTGGCAAA+TGG 0.527477 2.4:-13644283 MS.gene35690:CDS
GACCCCTTGCAGCTGGTCCA+CGG 0.529518 2.4:-13644204 MS.gene35690:CDS
TCCAAGAGAACAAGAAGCAA+TGG 0.530743 2.4:-13645126 MS.gene35690:CDS
CCACAGCCAAACTTGAACCT+TGG 0.532217 2.4:+13643917 None:intergenic
AGGAGAATGATCTCCATCAA+TGG 0.544643 2.4:+13645092 None:intergenic
TGAAGCAGCTGCAACCACTA+AGG 0.546688 2.4:+13644026 None:intergenic
TGAAGGAACTGAGTAAAGAA+AGG 0.555422 2.4:+13644913 None:intergenic
AATCCGTGGACCAGCTGCAA+GGG 0.560165 2.4:+13644201 None:intergenic
AACATGCAGGTTCTTTGTGG+TGG 0.566783 2.4:-13644290 MS.gene35690:intron
GGACTCAAGAAGAGAAGTTG+AGG 0.570390 2.4:-13645175 MS.gene35690:CDS
GGTTGTTTCTTTCGTTGCGT+CGG 0.570523 2.4:-13643896 MS.gene35690:CDS
TTGGTGCTTCTGTCATTATG+GGG 0.574068 2.4:-13644954 MS.gene35690:CDS
GCAGACAATTGAGAATGGTA+AGG 0.574191 2.4:+13644234 None:intergenic
ACAATGCAGACAATTGAGAA+TGG 0.577153 2.4:+13644229 None:intergenic
CCCATTGAGCATGAAACTGA+AGG 0.601992 2.4:+13644896 None:intergenic
GAATCCGTGGACCAGCTGCA+AGG 0.605753 2.4:+13644200 None:intergenic
AGCACCAAGAGTAGCACCAA+TGG 0.630653 2.4:+13644969 None:intergenic
GTTGTGTACTTAGCACACAA+TGG 0.633961 2.4:-13643991 MS.gene35690:CDS
ATCCGTGGACCAGCTGCAAG+GGG 0.635198 2.4:+13644202 None:intergenic
CAATTGAGAATGGTAAGGAG+AGG 0.647713 2.4:+13644239 None:intergenic
ACAACAGTTGCAGCATGTGG+AGG 0.650553 2.4:+13645072 None:intergenic
GTAACAACAGTTGCAGCATG+TGG 0.663640 2.4:+13645069 None:intergenic
ACGATGACAAGTAGAATCCG+TGG 0.676654 2.4:+13644187 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTTACAATTAATTGTATTTT+TGG - chr2.4:13644277-13644296 MS.gene35690:CDS 10.0%
!! AAAAAAAAAATGTTCTTAGA+GGG - chr2.4:13644521-13644540 MS.gene35690:intron 15.0%
!! AAACAATAAATTTCAAGAAT+AGG - chr2.4:13644471-13644490 MS.gene35690:intron 15.0%
!!! TTTAAATATCATCCTTTTTA+TGG - chr2.4:13644702-13644721 MS.gene35690:intron 15.0%
!! AATTGTGTTTCAGTTAAAAT+AGG + chr2.4:13644307-13644326 None:intergenic 20.0%
!! ATAGTATTGTAAAGATCAAA+TGG + chr2.4:13644198-13644217 None:intergenic 20.0%
!! GAAAAAAAAAATGTTCTTAG+AGG - chr2.4:13644520-13644539 MS.gene35690:intron 20.0%
!! TAGTATTGTAAAGATCAAAT+GGG + chr2.4:13644197-13644216 None:intergenic 20.0%
!!! AGAAAAAAGTTTTGAAACAA+GGG + chr2.4:13644364-13644383 None:intergenic 20.0%
! ACATGAACTTACTTAAACAT+TGG + chr2.4:13644158-13644177 None:intergenic 25.0%
! AGTAAGTTCATGTTACATAA+CGG - chr2.4:13644165-13644184 MS.gene35690:intron 25.0%
! TTACATCCGATAAATGAAAT+CGG - chr2.4:13644495-13644514 MS.gene35690:intron 25.0%
! TTTCTTCCGATTTCATTTAT+CGG + chr2.4:13644504-13644523 None:intergenic 25.0%
!! CAATCTTAGACTTCATTTTA+AGG - chr2.4:13644021-13644040 MS.gene35690:CDS 25.0%
!!! AACACTTTTTAAGAATGACT+TGG - chr2.4:13644246-13644265 MS.gene35690:CDS 25.0%
!!! GAAAAAAGTTTTGAAACAAG+GGG + chr2.4:13644363-13644382 None:intergenic 25.0%
!!! GAGAAAAAAGTTTTGAAACA+AGG + chr2.4:13644365-13644384 None:intergenic 25.0%
!!! TTTTCTCACAACTTAATTTG+AGG - chr2.4:13644378-13644397 MS.gene35690:intron 25.0%
AAATCTACTCCAATCCAAAA+AGG - chr2.4:13643978-13643997 MS.gene35690:CDS 30.0%
AACATGTCACGTCATAAAAT+AGG + chr2.4:13644407-13644426 None:intergenic 30.0%
CAAAAAGGTGGAATGAAAAA+GGG - chr2.4:13643993-13644012 MS.gene35690:CDS 30.0%
GCATGTTTAGATCCATAAAA+AGG + chr2.4:13644717-13644736 None:intergenic 30.0%
TACCTAATTGCAGGTATTAA+TGG - chr2.4:13644971-13644990 MS.gene35690:CDS 30.0%
TGTGTTTCAGTTAAAATAGG+AGG + chr2.4:13644304-13644323 None:intergenic 30.0%
! TTTTATGGATCTAAACATGC+AGG - chr2.4:13644717-13644736 MS.gene35690:intron 30.0%
!! ACGATATGAGAGTGTTTATT+TGG + chr2.4:13644553-13644572 None:intergenic 30.0%
!!! TAAACAATGTTTTGATGACG+TGG + chr2.4:13644586-13644605 None:intergenic 30.0%
!!! TTAGACTTCATTTTAAGGCT+TGG - chr2.4:13644026-13644045 MS.gene35690:CDS 30.0%
ACAATGCAGACAATTGAGAA+TGG + chr2.4:13644794-13644813 None:intergenic 35.0%
AGAACAAGAAGCAATGGAAA+AGG - chr2.4:13643900-13643919 MS.gene35690:CDS 35.0%
CCAAAAAGGTGGAATGAAAA+AGG - chr2.4:13643992-13644011 MS.gene35690:CDS 35.0%
CGAAATTGCAAGGATATGAA+GGG + chr2.4:13644658-13644677 None:intergenic 35.0%
GAACAAGAAGCAATGGAAAA+GGG - chr2.4:13643901-13643920 MS.gene35690:CDS 35.0%
TGAAGGAACTGAGTAAAGAA+AGG + chr2.4:13644110-13644129 None:intergenic 35.0%
TTCTTGTTCTCTTGGATTCT+AGG + chr2.4:13643890-13643909 None:intergenic 35.0%
TTGTGTACTTAGCACACAAT+GGG - chr2.4:13645030-13645049 MS.gene35690:CDS 35.0%
! TTCATTCCACCTTTTTGGAT+TGG + chr2.4:13643990-13644009 None:intergenic 35.0%
! TTTCCTATCTTGTTTGGTTG+TGG - chr2.4:13645148-13645167 MS.gene35690:CDS 35.0%
!! CCTTTTTCATTCCACCTTTT+TGG + chr2.4:13643995-13644014 None:intergenic 35.0%
!! TTTTTGCCAAGGTTCAAGTT+TGG - chr2.4:13645097-13645116 MS.gene35690:CDS 35.0%
AATGTTCGCTACCTAATTGC+AGG - chr2.4:13644962-13644981 MS.gene35690:CDS 40.0%
AATTGCAAGGATATGAAGGG+AGG + chr2.4:13644655-13644674 None:intergenic 40.0%
ACACCACAACCAAACAAGAT+AGG + chr2.4:13645154-13645173 None:intergenic 40.0%
AGGAGAATGATCTCCATCAA+TGG + chr2.4:13643931-13643950 None:intergenic 40.0%
CAATTGAGAATGGTAAGGAG+AGG + chr2.4:13644784-13644803 None:intergenic 40.0%
CCTTCAGTTTCATGCTCAAT+GGG - chr2.4:13644124-13644143 MS.gene35690:intron 40.0%
CTAAACATGCAGGTTCTTTG+TGG - chr2.4:13644727-13644746 MS.gene35690:intron 40.0%
CTATAGCAAGTAAAGATGCC+AGG + chr2.4:13644681-13644700 None:intergenic 40.0%
GCAGACAATTGAGAATGGTA+AGG + chr2.4:13644789-13644808 None:intergenic 40.0%
GCGAAATTGCAAGGATATGA+AGG + chr2.4:13644659-13644678 None:intergenic 40.0%
GGAAAAGGGAAATCCATTGA+TGG - chr2.4:13643915-13643934 MS.gene35690:CDS 40.0%
GGCCATTAATACCTGCAATT+AGG + chr2.4:13644976-13644995 None:intergenic 40.0%
GTTGTGTACTTAGCACACAA+TGG - chr2.4:13645029-13645048 MS.gene35690:CDS 40.0%
TCCAAGAGAACAAGAAGCAA+TGG - chr2.4:13643894-13643913 MS.gene35690:CDS 40.0%
TCCATTGCTTCTTGTTCTCT+TGG + chr2.4:13643898-13643917 None:intergenic 40.0%
TCCTTCAGTTTCATGCTCAA+TGG - chr2.4:13644123-13644142 MS.gene35690:intron 40.0%
TCTACTCCAATCCAAAAAGG+TGG - chr2.4:13643981-13644000 MS.gene35690:CDS 40.0%
TGTCTTCTGTAGCTCTCAAA+AGG - chr2.4:13645171-13645190 MS.gene35690:CDS 40.0%
! TGTTCTCTTGGATTCTAGGA+TGG + chr2.4:13643886-13643905 None:intergenic 40.0%
! TTGGTGCTTCTGTCATTATG+GGG - chr2.4:13644066-13644085 MS.gene35690:intron 40.0%
!! CTTGGTGCTTCTGTCATTAT+GGG - chr2.4:13644065-13644084 MS.gene35690:intron 40.0%
!! GTCGGTTTTCCTATCTTGTT+TGG - chr2.4:13645142-13645161 MS.gene35690:CDS 40.0%
!! GTTCTCTTGGATTCTAGGAT+GGG + chr2.4:13643885-13643904 None:intergenic 40.0%
!! TCTTGGTGCTTCTGTCATTA+TGG - chr2.4:13644064-13644083 MS.gene35690:intron 40.0%
!!! ATTTTAAGGCTTGGTGCCAT+TGG - chr2.4:13644035-13644054 MS.gene35690:CDS 40.0%
!!! CAGTTCACTGATTTTTGCCA+AGG - chr2.4:13645086-13645105 MS.gene35690:CDS 40.0%
AACATGCAGGTTCTTTGTGG+TGG - chr2.4:13644730-13644749 MS.gene35690:intron 45.0%
ACGATGACAAGTAGAATCCG+TGG + chr2.4:13644836-13644855 None:intergenic 45.0%
AGGTTCTTTGTGGTGGCAAA+TGG - chr2.4:13644737-13644756 MS.gene35690:intron 45.0%
ATGGGAGTCAAGATGCAAAC+TGG - chr2.4:13645048-13645067 MS.gene35690:CDS 45.0%
CATATCCTTGCAATTTCGCC+TGG - chr2.4:13644660-13644679 MS.gene35690:intron 45.0%
CCCATTGAGCATGAAACTGA+AGG + chr2.4:13644127-13644146 None:intergenic 45.0%
GGACTCAAGAAGAGAAGTTG+AGG - chr2.4:13643845-13643864 MS.gene35690:CDS 45.0%
GTAACAACAGTTGCAGCATG+TGG + chr2.4:13643954-13643973 None:intergenic 45.0%
! GCAGGTATTAATGGCCTTAG+TGG - chr2.4:13644980-13644999 MS.gene35690:CDS 45.0%
!! GGTTGTTTCTTTCGTTGCGT+CGG - chr2.4:13645124-13645143 MS.gene35690:CDS 45.0%
ACAACAGTTGCAGCATGTGG+AGG + chr2.4:13643951-13643970 None:intergenic 50.0%
AGATGCCAGGCGAAATTGCA+AGG + chr2.4:13644668-13644687 None:intergenic 50.0%
AGCACCAAGAGTAGCACCAA+TGG + chr2.4:13644054-13644073 None:intergenic 50.0%
ATTGTTAGACCCCTTGCAGC+TGG - chr2.4:13644809-13644828 MS.gene35690:intron 50.0%
CCACAGCCAAACTTGAACCT+TGG + chr2.4:13645106-13645125 None:intergenic 50.0%
TGAAGCAGCTGCAACCACTA+AGG + chr2.4:13644997-13645016 None:intergenic 50.0%
! CCAAGGTTCAAGTTTGGCTG+TGG - chr2.4:13645103-13645122 MS.gene35690:CDS 50.0%
AATCCGTGGACCAGCTGCAA+GGG + chr2.4:13644822-13644841 None:intergenic 55.0%
!! GGTGCCATTGGTGCTACTCT+TGG - chr2.4:13644047-13644066 MS.gene35690:intron 55.0%
ATCCGTGGACCAGCTGCAAG+GGG + chr2.4:13644821-13644840 None:intergenic 60.0%
GAATCCGTGGACCAGCTGCA+AGG + chr2.4:13644823-13644842 None:intergenic 60.0%
GTGGCAAATGGAGCAGCAGC+TGG - chr2.4:13644749-13644768 MS.gene35690:intron 60.0%
GACCCCTTGCAGCTGGTCCA+CGG - chr2.4:13644816-13644835 MS.gene35690:intron 65.0%


Chromosome Type Strat End Strand Name
chr2.4 gene 13643843 13645199 13643843 ID=MS.gene35690
chr2.4 mRNA 13643843 13645199 13643843 ID=MS.gene35690.t1;Parent=MS.gene35690
chr2.4 exon 13644877 13645199 13644877 ID=MS.gene35690.t1.exon1;Parent=MS.gene35690.t1
chr2.4 CDS 13644877 13645199 13644877 ID=cds.MS.gene35690.t1;Parent=MS.gene35690.t1
chr2.4 exon 13644180 13644303 13644180 ID=MS.gene35690.t1.exon2;Parent=MS.gene35690.t1
chr2.4 CDS 13644180 13644303 13644180 ID=cds.MS.gene35690.t1;Parent=MS.gene35690.t1
chr2.4 exon 13643843 13644058 13643843 ID=MS.gene35690.t1.exon3;Parent=MS.gene35690.t1
chr2.4 CDS 13643843 13644058 13643843 ID=cds.MS.gene35690.t1;Parent=MS.gene35690.t1
Gene Sequence

>MS.gene35690

ATGGACTCAAGAAGAGAAGTTGAGGAATCATCAACAGCTCCCATCCTAGAATCCAAGAGAACAAGAAGCAATGGAAAAGGGAAATCCATTGATGGAGATCATTCTCCTCCACATGCTGCAACTGTTGTTACAACAAAATCTACTCCAATCCAAAAAGGTGGAATGAAAAAGGGAATTGCAATCTTAGACTTCATTTTAAGGCTTGGTGCCATTGGTGCTACTCTTGGTGCTTCTGTCATTATGGGGACTAATGAACAAATACTTCCTTTCTTTACTCAGTTCCTTCAGTTTCATGCTCAATGGGATGATTTTCCAATGTTTAAGTAAGTTCATGTTACATAACGGTGATATCCCATTTGATCTTTACAATACTATTATACATTTTCAGTTTTATTTTTATAATAACACTTTTTAAGAATGACTTGGTGTACAATTTTACAATTAATTGTATTTTTGGTCCTCCTATTTTAACTGAAACACAATTTTAGACTCTCATTTCTAAATTCAGAATTTTAGTCCCCTTGTTTCAAAACTTTTTTCTCACAACTTAATTTGAGGTCTCCTATTTTATGACGTGACATGTTTATTAATGACGTGAAATTAAATTTGATGACTCGATTTGAATTAGAAACAATAAATTTCAAGAATAGGTTTACATCCGATAAATGAAATCGGAAGAAAAAAAAAATGTTCTTAGAGGGTTCTCTCCAAATAAACACTCTCATATCGTCAAATGTAATCCACGTCATCAAAACATTGTTTAAGATAAAAATAGAGAAATTAAATGTGCATATAATTGTTTTTTTTTTCCTCCCTTCATATCCTTGCAATTTCGCCTGGCATCTTTACTTGCTATAGATTTAAATATCATCCTTTTTATGGATCTAAACATGCAGGTTCTTTGTGGTGGCAAATGGAGCAGCAGCTGGATTTCTCATCCTCTCCTTACCATTCTCAATTGTCTGCATTGTTAGACCCCTTGCAGCTGGTCCACGGATTCTACTTGTCATCGTTGATTTAGTAAGCAAAAATGCAATATGATTAATGATAATGCACTAATCAATAATCATCATTATATCTTATTTCAAATAATCATTCAAAACTTAACTAGAAAAAAAAAATGTTCGCTACCTAATTGCAGGTATTAATGGCCTTAGTGGTTGCAGCTGCTTCATCTGCTGCAGCTGTTGTGTACTTAGCACACAATGGGAGTCAAGATGCAAACTGGAATGCTATTTGTCAGCAGTTCACTGATTTTTGCCAAGGTTCAAGTTTGGCTGTGGTTGTTTCTTTCGTTGCGTCGGTTTTCCTATCTTGTTTGGTTGTGGTGTCTTCTGTAGCTCTCAAAAGGACTTAA

Protein sequence

>MS.gene35690.t1

MDSRREVEESSTAPILESKRTRSNGKGKSIDGDHSPPHAATVVTTKSTPIQKGGMKKGIAILDFILRLGAIGATLGASVIMGTNEQILPFFTQFLQFHAQWDDFPMFKFFVVANGAAAGFLILSLPFSIVCIVRPLAAGPRILLVIVDLVLMALVVAAASSAAAVVYLAHNGSQDANWNAICQQFTDFCQGSSLAVVVSFVASVFLSCLVVVSSVALKRT