AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene31609


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene31609.t1 MTR_1g008500 82.000 300 20 3 1 266 86 385 1.75e-173 484
MS.gene31609.t1 MTR_1g008590 75.096 261 55 3 6 266 75 325 2.37e-141 400
MS.gene31609.t1 MTR_0312s0030 74.060 266 59 3 1 266 70 325 5.39e-141 399
MS.gene31609.t1 MTR_1g007520 74.060 266 59 3 1 266 70 325 5.39e-141 399
MS.gene31609.t1 MTR_1g008510 79.832 238 43 2 31 266 92 326 8.43e-138 391
MS.gene31609.t1 MTR_1g008560 72.388 268 67 3 3 266 66 330 1.07e-134 383
MS.gene31609.t1 MTR_1g008530 78.995 219 45 1 49 266 6 224 3.17e-128 363
MS.gene31609.t1 MTR_1g008470 73.617 235 59 3 32 266 69 300 2.84e-125 358
MS.gene31609.t1 MTR_1g008450 72.650 234 61 3 33 266 83 313 1.82e-119 344
MS.gene31609.t1 MTR_1g008460 63.878 263 86 4 4 266 44 297 2.13e-115 333
MS.gene31609.t1 MTR_3g109490 57.407 270 102 5 2 264 57 320 1.85e-108 317
MS.gene31609.t1 MTR_1g008580 81.871 171 31 0 95 265 1 171 6.70e-104 299
MS.gene31609.t1 MTR_4g069680 43.304 224 122 4 47 266 65 287 4.36e-62 197
MS.gene31609.t1 MTR_3g116410 43.750 224 121 4 47 266 54 276 3.94e-60 192
MS.gene31609.t1 MTR_3g107830 43.304 224 122 3 47 266 34 256 8.12e-60 191
MS.gene31609.t1 MTR_3g116380 43.498 223 118 5 47 264 82 301 1.41e-58 189
MS.gene31609.t1 MTR_3g116320 41.284 218 118 5 52 264 474 686 2.51e-47 167
MS.gene31609.t1 MTR_3g116270 38.991 218 123 5 52 264 292 504 1.18e-46 163
MS.gene31609.t1 MTR_4g069570 36.986 219 128 6 52 264 120 334 1.37e-44 153
MS.gene31609.t1 MTR_4g069040 35.865 237 141 7 34 264 277 508 1.09e-43 155
MS.gene31609.t1 MTR_8g064500 36.530 219 129 5 52 265 159 372 1.18e-41 147
MS.gene31609.t1 MTR_4g069550 36.986 219 128 6 52 264 247 461 1.69e-41 148
MS.gene31609.t1 MTR_4g069520 36.530 219 129 6 52 264 507 721 1.69e-39 145
MS.gene31609.t1 MTR_4g069520 36.530 219 129 6 52 264 493 707 2.07e-39 145
MS.gene31609.t1 MTR_8g064560 35.160 219 132 5 52 265 412 625 3.75e-38 141
MS.gene31609.t1 MTR_8g064530 34.703 219 133 6 52 265 429 642 2.37e-36 136
MS.gene31609.t1 MTR_5g034320 33.180 217 137 5 52 264 415 627 2.95e-36 136
MS.gene31609.t1 MTR_3g078090 36.649 191 114 4 79 265 439 626 3.35e-35 133
MS.gene31609.t1 MTR_8g445170 40.000 150 83 3 115 264 240 382 2.70e-33 125
MS.gene31609.t1 MTR_6g086530 33.333 195 122 6 79 266 13 206 7.03e-33 119
MS.gene31609.t1 MTR_8g445140 30.469 256 120 7 53 264 60 301 2.36e-32 120
MS.gene31609.t1 MTR_1g008420 75.610 82 18 2 38 118 119 199 6.44e-32 117
MS.gene31609.t1 MTR_8g046000 35.000 220 109 8 51 264 30 221 6.96e-32 117
MS.gene31609.t1 MTR_8g045890 43.056 144 75 4 121 264 294 430 1.14e-31 121
MS.gene31609.t1 MTR_8g045890 43.056 144 75 4 121 264 335 471 2.31e-31 121
MS.gene31609.t1 MTR_2g081610 34.579 214 111 7 52 264 73 258 2.77e-31 117
MS.gene31609.t1 MTR_4g133840 33.511 188 106 4 79 263 15 186 2.79e-31 115
MS.gene31609.t1 MTR_8g045890 43.056 144 75 4 121 264 372 508 3.05e-31 121
MS.gene31609.t1 MTR_8g045880 41.781 146 78 3 119 264 175 313 1.47e-30 116
MS.gene31609.t1 MTR_8g045880 41.781 146 78 3 119 264 210 348 2.24e-30 117
MS.gene31609.t1 MTR_8g045880 41.781 146 78 3 119 264 212 350 2.96e-30 116
MS.gene31609.t1 MTR_2g081770 32.093 215 120 6 52 264 79 269 7.20e-29 110
MS.gene31609.t1 MTR_2g081590 32.710 214 115 7 52 264 73 258 7.84e-29 110
MS.gene31609.t1 MTR_5g033340 30.455 220 145 5 50 265 164 379 1.37e-27 109
MS.gene31609.t1 MTR_5g033340 32.215 149 95 3 102 247 8 153 5.45e-16 77.4
MS.gene31609.t1 MTR_1g045860 32.110 218 115 7 52 264 69 258 3.70e-26 103
MS.gene31609.t1 MTR_5g033350 31.250 192 125 4 78 265 12 200 5.65e-26 101
MS.gene31609.t1 MTR_5g033330 30.275 218 144 5 50 263 403 616 5.87e-25 103
MS.gene31609.t1 MTR_5g033330 30.198 202 133 5 50 247 195 392 8.57e-21 92.0
MS.gene31609.t1 MTR_5g033330 31.034 174 113 4 78 247 14 184 9.53e-18 82.8
MS.gene31609.t1 MTR_8g044290 44.545 110 55 3 121 230 229 332 1.70e-23 99.4
MS.gene31609.t1 MTR_5g033360 28.311 219 133 6 50 265 25 222 1.23e-21 90.5
MS.gene31609.t1 MTR_4g068420 35.294 136 68 5 132 264 1 119 6.07e-20 83.6
MS.gene31609.t1 MTR_8g075930 40.506 79 43 2 179 254 10 87 2.61e-18 77.8
MS.gene31609.t1 MTR_5g033430 33.333 87 56 2 181 265 24 110 2.82e-13 65.5
MS.gene31609.t1 MTR_5g033440 32.184 87 57 2 181 265 24 110 1.42e-12 65.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene31609.t1 AT5G25610 56.746 252 100 5 22 265 141 391 2.96e-97 290
MS.gene31609.t1 AT1G49320 37.662 231 134 5 45 266 51 280 9.22e-50 165
MS.gene31609.t1 AT1G60390 35.321 218 129 6 52 263 409 620 3.24e-37 139
MS.gene31609.t1 AT1G70370 34.562 217 134 4 52 264 411 623 2.93e-36 136
MS.gene31609.t1 AT1G70370 34.562 217 134 4 52 264 411 623 2.93e-36 136
MS.gene31609.t1 AT1G23760 33.641 217 136 5 52 264 407 619 3.35e-34 130

Find 40 sgRNAs with CRISPR-Local

Find 47 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ATTGAATTAGCAACTTCTTT+AGG 0.259477 1.1:+10996531 None:intergenic
TTCTTCTGTTCCTTCTTTGA+TGG 0.265021 1.1:+10996464 None:intergenic
GGCAAAAGGTGTGAAGAAAT+TGG 0.273962 1.1:-10996259 MS.gene31609:CDS
TGTACTTTGCTTGAAGAAAA+AGG 0.274463 1.1:+10996507 None:intergenic
TTCCACAGGATCATGTTGTT+TGG 0.285326 1.1:-10996005 MS.gene31609:CDS
ACTTGAAAAGCCAAATGTTT+AGG 0.303666 1.1:+10996069 None:intergenic
TCCACAGGATCATGTTGTTT+GGG 0.305699 1.1:-10996004 MS.gene31609:CDS
TGTGACATCATTGGAATCTA+TGG 0.323363 1.1:-10996373 MS.gene31609:CDS
ATAAGTGAGTGTGAAGAAAA+TGG 0.348496 1.1:-10996420 MS.gene31609:CDS
GTGCCTTTGGAAGGTGTTGA+TGG 0.355685 1.1:-10996144 MS.gene31609:CDS
AAGAAGAAAAGTGCTACATT+TGG 0.370145 1.1:+10996633 None:intergenic
ACAATGGAATCCTAAACATT+TGG 0.370419 1.1:-10996079 MS.gene31609:CDS
TAAAGTTTACTCTGTGCCTT+TGG 0.380290 1.1:-10996157 MS.gene31609:CDS
TTGAAAAGTTCTCAATTACT+TGG 0.383116 1.1:+10995978 None:intergenic
AAAGCTTTGTGTGACATCAT+TGG 0.404983 1.1:-10996382 MS.gene31609:CDS
CTTTGATGGAGAACATGTTA+AGG 0.426299 1.1:+10996478 None:intergenic
GCAAAAGGTGTGAAGAAATT+GGG 0.438714 1.1:-10996258 MS.gene31609:CDS
CAAGAATATGTAATGGCAAA+AGG 0.445313 1.1:-10996273 MS.gene31609:CDS
TGTGGAAGGAAGTGACAAAC+TGG 0.450964 1.1:+10996021 None:intergenic
CAAGTGCTCAATGTTCAACC+TGG 0.454270 1.1:-10996051 MS.gene31609:CDS
GATTTCACTACTTCGAAACT+TGG 0.458312 1.1:-10996348 MS.gene31609:CDS
TTAGAAAAGGATTTACATCA+AGG 0.470233 1.1:-10996612 MS.gene31609:CDS
AACTATATGAATGTTGATGC+AGG 0.499341 1.1:-10996777 None:intergenic
AGTTTGTCACTTCCTTCCAC+AGG 0.501409 1.1:-10996019 MS.gene31609:CDS
ATTAAAGGACAACCAGGAAA+AGG 0.504978 1.1:-10996711 MS.gene31609:CDS
GTTTACTCTGTGCCTTTGGA+AGG 0.519063 1.1:-10996153 MS.gene31609:CDS
TGTGAAGAAAATGGCATCAA+AGG 0.529052 1.1:-10996411 MS.gene31609:CDS
AACATGTTCTCCATCAAAGA+AGG 0.536136 1.1:-10996474 MS.gene31609:CDS
TGACAAACTGGAACAGTCCC+AGG 0.541759 1.1:+10996033 None:intergenic
ACACAACAACAGCTTTAACT+CGG 0.579233 1.1:+10996118 None:intergenic
TGACTTGCAAGAATATGTAA+TGG 0.583650 1.1:-10996280 MS.gene31609:CDS
GCAGCATAATTGTAAAACAT+AGG 0.586082 1.1:+10996681 None:intergenic
AAAGGACAACCAGGAAAAGG+TGG 0.597740 1.1:-10996708 MS.gene31609:CDS
AAGTGCTCAATGTTCAACCT+GGG 0.607659 1.1:-10996050 MS.gene31609:CDS
CTTCCATCAACACCTTCCAA+AGG 0.627468 1.1:+10996141 None:intergenic
AAACAACATGATCCTGTGGA+AGG 0.641220 1.1:+10996007 None:intergenic
AGAAAATGGCATCAAAGGAG+AGG 0.642414 1.1:-10996406 MS.gene31609:CDS
AAAGATATTAAAGGACAACC+AGG 0.645079 1.1:-10996717 MS.gene31609:CDS
ACCCAAACAACATGATCCTG+TGG 0.647573 1.1:+10996003 None:intergenic
GTCACACTGATACTTCACAA+TGG 0.703629 1.1:-10996095 MS.gene31609:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AACATAGAAAAAGATATTAA+AGG - chr1.1:10996038-10996057 MS.gene31609:CDS 15.0%
! ATTGAATTAGCAACTTCTTT+AGG + chr1.1:10996236-10996255 None:intergenic 25.0%
! TTAGAAAAGGATTTACATCA+AGG - chr1.1:10996152-10996171 MS.gene31609:CDS 25.0%
AAAGATATTAAAGGACAACC+AGG - chr1.1:10996047-10996066 MS.gene31609:CDS 30.0%
ACAATGGAATCCTAAACATT+TGG - chr1.1:10996685-10996704 MS.gene31609:CDS 30.0%
ACTTGAAAAGCCAAATGTTT+AGG + chr1.1:10996698-10996717 None:intergenic 30.0%
ATAAGTGAGTGTGAAGAAAA+TGG - chr1.1:10996344-10996363 MS.gene31609:CDS 30.0%
CAAGAATATGTAATGGCAAA+AGG - chr1.1:10996491-10996510 MS.gene31609:CDS 30.0%
GACAGTAAAAAACTGCATAA+GGG + chr1.1:10996571-10996590 None:intergenic 30.0%
GCAGCATAATTGTAAAACAT+AGG + chr1.1:10996086-10996105 None:intergenic 30.0%
TGACAGTAAAAAACTGCATA+AGG + chr1.1:10996572-10996591 None:intergenic 30.0%
TGTACTTTGCTTGAAGAAAA+AGG + chr1.1:10996260-10996279 None:intergenic 30.0%
! AGCACTTTTCTTCTTAGAAA+AGG - chr1.1:10996139-10996158 MS.gene31609:CDS 30.0%
! TGACTTGCAAGAATATGTAA+TGG - chr1.1:10996484-10996503 MS.gene31609:CDS 30.0%
! TTTTGCTTCATCATTTGAGT+TGG + chr1.1:10996207-10996226 None:intergenic 30.0%
!! AAGAAGAAAAGTGCTACATT+TGG + chr1.1:10996134-10996153 None:intergenic 30.0%
AACATGTTCTCCATCAAAGA+AGG - chr1.1:10996290-10996309 MS.gene31609:CDS 35.0%
ATGCAGGAAAAACAAAGTCA+AGG - chr1.1:10996003-10996022 MS.gene31609:CDS 35.0%
ATTAAAGGACAACCAGGAAA+AGG - chr1.1:10996053-10996072 MS.gene31609:CDS 35.0%
CTTTGATGGAGAACATGTTA+AGG + chr1.1:10996289-10996308 None:intergenic 35.0%
GATTTCACTACTTCGAAACT+TGG - chr1.1:10996416-10996435 MS.gene31609:CDS 35.0%
TAAAGTTTACTCTGTGCCTT+TGG - chr1.1:10996607-10996626 MS.gene31609:CDS 35.0%
TGCAGGAAAAACAAAGTCAA+GGG - chr1.1:10996004-10996023 MS.gene31609:CDS 35.0%
TGTGAAGAAAATGGCATCAA+AGG - chr1.1:10996353-10996372 MS.gene31609:CDS 35.0%
TGTGACATCATTGGAATCTA+TGG - chr1.1:10996391-10996410 MS.gene31609:CDS 35.0%
TTCTTCTGTTCCTTCTTTGA+TGG + chr1.1:10996303-10996322 None:intergenic 35.0%
! AAAGCTTTGTGTGACATCAT+TGG - chr1.1:10996382-10996401 MS.gene31609:CDS 35.0%
! ACACAACAACAGCTTTAACT+CGG + chr1.1:10996649-10996668 None:intergenic 35.0%
! GCAAAAGGTGTGAAGAAATT+GGG - chr1.1:10996506-10996525 MS.gene31609:CDS 35.0%
AAACAACATGATCCTGTGGA+AGG + chr1.1:10996760-10996779 None:intergenic 40.0%
AAGTGCTCAATGTTCAACCT+GGG - chr1.1:10996714-10996733 MS.gene31609:CDS 40.0%
AGAAAATGGCATCAAAGGAG+AGG - chr1.1:10996358-10996377 MS.gene31609:CDS 40.0%
GCAGGAAAAACAAAGTCAAG+GGG - chr1.1:10996005-10996024 MS.gene31609:CDS 40.0%
GTCACACTGATACTTCACAA+TGG - chr1.1:10996669-10996688 MS.gene31609:CDS 40.0%
TTCCACAGGATCATGTTGTT+TGG - chr1.1:10996759-10996778 MS.gene31609:CDS 40.0%
! ATAGGAAAACCACCTTTTCC+TGG + chr1.1:10996068-10996087 None:intergenic 40.0%
! GGCAAAAGGTGTGAAGAAAT+TGG - chr1.1:10996505-10996524 MS.gene31609:CDS 40.0%
! TCCACAGGATCATGTTGTTT+GGG - chr1.1:10996760-10996779 MS.gene31609:CDS 40.0%
AAAGGACAACCAGGAAAAGG+TGG - chr1.1:10996056-10996075 MS.gene31609:CDS 45.0%
ACCCAAACAACATGATCCTG+TGG + chr1.1:10996764-10996783 None:intergenic 45.0%
CAAGTGCTCAATGTTCAACC+TGG - chr1.1:10996713-10996732 MS.gene31609:CDS 45.0%
CTTCCATCAACACCTTCCAA+AGG + chr1.1:10996626-10996645 None:intergenic 45.0%
GTTTACTCTGTGCCTTTGGA+AGG - chr1.1:10996611-10996630 MS.gene31609:CDS 45.0%
TGTGGAAGGAAGTGACAAAC+TGG + chr1.1:10996746-10996765 None:intergenic 45.0%
! AGTTTGTCACTTCCTTCCAC+AGG - chr1.1:10996745-10996764 MS.gene31609:CDS 45.0%
TGACAAACTGGAACAGTCCC+AGG + chr1.1:10996734-10996753 None:intergenic 50.0%
!! GTGCCTTTGGAAGGTGTTGA+TGG - chr1.1:10996620-10996639 MS.gene31609:CDS 50.0%


Chromosome Type Strat End Strand Name
chr1.1 gene 10995993 10996793 10995993 ID=MS.gene31609
chr1.1 mRNA 10995993 10996793 10995993 ID=MS.gene31609.t1;Parent=MS.gene31609
chr1.1 exon 10995993 10996793 10995993 ID=MS.gene31609.t1.exon1;Parent=MS.gene31609.t1
chr1.1 CDS 10995993 10996793 10995993 ID=cds.MS.gene31609.t1;Parent=MS.gene31609.t1
Gene Sequence

>MS.gene31609

ATGAATGTTGATGCAGGAAAAACAAAGTCAAGGGGAACATCAGTCAACATAGAAAAAGATATTAAAGGACAACCAGGAAAAGGTGGTTTTCCTATGTTTTACAATTATGCTGCAAGTGAGACACAATTACATGATAAACCAAATGTAGCACTTTTCTTCTTAGAAAAGGATTTACATCAAGGAACAAAGTTGAACTTGCAATTCACAAAATCCAACTCAAATGATGAAGCAAAATTCTTACCTAAAGAAGTTGCTAATTCAATACCTTTTTCTTCAAGCAAAGTACAAAACATCCTTAACATGTTCTCCATCAAAGAAGGAACAGAAGAATCTGAAATTGTGAAGAACACAATAAGTGAGTGTGAAGAAAATGGCATCAAAGGAGAGGAAAAGCTTTGTGTGACATCATTGGAATCTATGGTTGATTTCACTACTTCGAAACTTGGAAACAATGTTGAAGCTGTCTCAACAGAAGTGAAAAAAGAAAGCAGTGACTTGCAAGAATATGTAATGGCAAAAGGTGTGAAGAAATTGGGAGAGAAGAATAAAGCTGTAGTGTGTCACAAAGAGAATTACCCTTATGCAGTTTTTTACTGTCATAAAACAGATTCAATTAAAGTTTACTCTGTGCCTTTGGAAGGTGTTGATGGAAGCCGAGTTAAAGCTGTTGTTGTGTGTCACACTGATACTTCACAATGGAATCCTAAACATTTGGCTTTTCAAGTGCTCAATGTTCAACCTGGGACTGTTCCAGTTTGTCACTTCCTTCCACAGGATCATGTTGTTTGGGTTTCCAAGTAA

Protein sequence

>MS.gene31609.t1

MNVDAGKTKSRGTSVNIEKDIKGQPGKGGFPMFYNYAASETQLHDKPNVALFFLEKDLHQGTKLNLQFTKSNSNDEAKFLPKEVANSIPFSSSKVQNILNMFSIKEGTEESEIVKNTISECEENGIKGEEKLCVTSLESMVDFTTSKLGNNVEAVSTEVKKESSDLQEYVMAKGVKKLGEKNKAVVCHKENYPYAVFYCHKTDSIKVYSVPLEGVDGSRVKAVVVCHTDTSQWNPKHLAFQVLNVQPGTVPVCHFLPQDHVVWVSK