Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene44649.t1 | XP_003621474.2 | 90.2 | 459 | 44 | 1 | 1 | 459 | 1 | 458 | 1.20E-238 | 835.5 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene44649.t1 | Q940Z5 | 38.0 | 471 | 262 | 13 | 3 | 457 | 11 | 467 | 2.7e-82 | 307.4 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene44649.t1 | G7L664 | 90.2 | 459 | 44 | 1 | 1 | 459 | 1 | 458 | 8.6e-239 | 835.5 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049150 | MS.gene44649 | 0.842669 | 2.24E-58 | -1.69E-46 |
| MS.gene049153 | MS.gene44649 | 0.829923 | 3.92E-55 | -1.69E-46 |
| MS.gene049233 | MS.gene44649 | 0.802325 | 5.92E-49 | -1.69E-46 |
| MS.gene049260 | MS.gene44649 | 0.830378 | 3.04E-55 | -1.69E-46 |
| MS.gene04933 | MS.gene44649 | 0.809261 | 2.06E-50 | -1.69E-46 |
| MS.gene049681 | MS.gene44649 | 0.831887 | 1.29E-55 | -1.69E-46 |
| MS.gene050516 | MS.gene44649 | 0.806118 | 9.61E-50 | -1.69E-46 |
| MS.gene050762 | MS.gene44649 | 0.802402 | 5.71E-49 | -1.69E-46 |
| MS.gene050763 | MS.gene44649 | 0.804058 | 2.59E-49 | -1.69E-46 |
| MS.gene050787 | MS.gene44649 | 0.82981 | 4.18E-55 | -1.69E-46 |
| MS.gene051860 | MS.gene44649 | 0.857526 | 1.54E-62 | -1.69E-46 |
| MS.gene052011 | MS.gene44649 | -0.811847 | 5.71E-51 | -1.69E-46 |
| MS.gene052207 | MS.gene44649 | 0.80655 | 7.79E-50 | -1.69E-46 |
| MS.gene052711 | MS.gene44649 | 0.804798 | 1.82E-49 | -1.69E-46 |
| MS.gene053074 | MS.gene44649 | 0.807954 | 3.93E-50 | -1.69E-46 |
| MS.gene053147 | MS.gene44649 | 0.809444 | 1.89E-50 | -1.69E-46 |
| MS.gene053555 | MS.gene44649 | 0.808353 | 3.23E-50 | -1.69E-46 |
| MS.gene053811 | MS.gene44649 | 0.833891 | 4.11E-56 | -1.69E-46 |
| MS.gene053836 | MS.gene44649 | 0.827876 | 1.23E-54 | -1.69E-46 |
| MS.gene054010 | MS.gene44649 | 0.854997 | 8.48E-62 | -1.69E-46 |
| MS.gene054011 | MS.gene44649 | 0.857216 | 1.90E-62 | -1.69E-46 |
| MS.gene054012 | MS.gene44649 | 0.853982 | 1.67E-61 | -1.69E-46 |
| MS.gene054013 | MS.gene44649 | 0.850939 | 1.23E-60 | -1.69E-46 |
| MS.gene054173 | MS.gene44649 | 0.814407 | 1.57E-51 | -1.69E-46 |
| MS.gene054183 | MS.gene44649 | 0.852462 | 4.55E-61 | -1.69E-46 |
| MS.gene054468 | MS.gene44649 | 0.81484 | 1.26E-51 | -1.69E-46 |
| MS.gene054473 | MS.gene44649 | 0.821383 | 4.16E-53 | -1.69E-46 |
| MS.gene054581 | MS.gene44649 | 0.801411 | 9.12E-49 | -1.69E-46 |
| MS.gene055356 | MS.gene44649 | 0.84295 | 1.89E-58 | -1.69E-46 |
| MS.gene055358 | MS.gene44649 | 0.877293 | 7.06E-69 | -1.69E-46 |
| MS.gene055604 | MS.gene44649 | 0.878408 | 2.88E-69 | -1.69E-46 |
| MS.gene056902 | MS.gene44649 | 0.861258 | 1.16E-63 | -1.69E-46 |
| MS.gene057469 | MS.gene44649 | 0.882701 | 8.35E-71 | -1.69E-46 |
| MS.gene057476 | MS.gene44649 | 0.809391 | 1.94E-50 | -1.69E-46 |
| MS.gene057826 | MS.gene44649 | 0.814909 | 1.21E-51 | -1.69E-46 |
| MS.gene057929 | MS.gene44649 | 0.830477 | 2.87E-55 | -1.69E-46 |
| MS.gene05811 | MS.gene44649 | 0.808597 | 2.86E-50 | -1.69E-46 |
| MS.gene058710 | MS.gene44649 | 0.804768 | 1.84E-49 | -1.69E-46 |
| MS.gene058949 | MS.gene44649 | 0.830792 | 2.41E-55 | -1.69E-46 |
| MS.gene05907 | MS.gene44649 | 0.801418 | 9.09E-49 | -1.69E-46 |
| MS.gene059294 | MS.gene44649 | 0.813882 | 2.04E-51 | -1.69E-46 |
| MS.gene059420 | MS.gene44649 | 0.825941 | 3.56E-54 | -1.69E-46 |
| MS.gene059609 | MS.gene44649 | 0.823209 | 1.57E-53 | -1.69E-46 |
| MS.gene05984 | MS.gene44649 | 0.806138 | 9.51E-50 | -1.69E-46 |
| MS.gene060314 | MS.gene44649 | 0.801607 | 8.31E-49 | -1.69E-46 |
| MS.gene060802 | MS.gene44649 | 0.807693 | 4.46E-50 | -1.69E-46 |
| MS.gene061049 | MS.gene44649 | 0.801833 | 7.47E-49 | -1.69E-46 |
| MS.gene061114 | MS.gene44649 | -0.813662 | 2.28E-51 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene44649.t1 | MTR_7g014330 | 90.196 | 459 | 44 | 1 | 1 | 459 | 1 | 458 | 0.0 | 853 |
| MS.gene44649.t1 | MTR_7g014250 | 87.800 | 459 | 55 | 1 | 1 | 459 | 1 | 458 | 0.0 | 829 |
| MS.gene44649.t1 | MTR_7g014230 | 88.791 | 455 | 50 | 1 | 2 | 456 | 4 | 457 | 0.0 | 827 |
| MS.gene44649.t1 | MTR_7g014360 | 81.701 | 388 | 50 | 2 | 72 | 459 | 22 | 388 | 0.0 | 638 |
| MS.gene44649.t1 | MTR_7g014310 | 74.627 | 335 | 30 | 3 | 108 | 442 | 29 | 308 | 3.83e-171 | 483 |
| MS.gene44649.t1 | MTR_6g015815 | 47.234 | 470 | 221 | 13 | 2 | 459 | 8 | 462 | 2.37e-144 | 421 |
| MS.gene44649.t1 | MTR_6g015850 | 46.996 | 466 | 223 | 12 | 3 | 459 | 9 | 459 | 8.25e-141 | 412 |
| MS.gene44649.t1 | MTR_6g015830 | 46.581 | 468 | 225 | 12 | 3 | 459 | 9 | 462 | 2.59e-139 | 408 |
| MS.gene44649.t1 | MTR_6g015320 | 46.552 | 464 | 225 | 11 | 3 | 459 | 9 | 456 | 1.83e-138 | 405 |
| MS.gene44649.t1 | MTR_6g015315 | 45.022 | 462 | 236 | 10 | 3 | 459 | 6 | 454 | 1.30e-132 | 390 |
| MS.gene44649.t1 | MTR_7g014610 | 39.623 | 477 | 260 | 11 | 3 | 459 | 9 | 477 | 8.10e-110 | 333 |
| MS.gene44649.t1 | MTR_7g014200 | 40.890 | 472 | 253 | 14 | 3 | 459 | 10 | 470 | 2.24e-108 | 329 |
| MS.gene44649.t1 | MTR_7g014160 | 40.546 | 476 | 252 | 15 | 3 | 458 | 10 | 474 | 1.75e-105 | 322 |
| MS.gene44649.t1 | MTR_7g013850 | 37.118 | 458 | 256 | 12 | 27 | 458 | 30 | 481 | 2.69e-93 | 291 |
| MS.gene44649.t1 | MTR_7g014620 | 39.150 | 447 | 236 | 11 | 22 | 457 | 11 | 432 | 4.43e-90 | 281 |
| MS.gene44649.t1 | MTR_2g089765 | 37.712 | 472 | 263 | 13 | 3 | 453 | 17 | 478 | 2.38e-84 | 268 |
| MS.gene44649.t1 | MTR_3g028210 | 34.188 | 468 | 283 | 11 | 3 | 457 | 9 | 464 | 1.53e-79 | 254 |
| MS.gene44649.t1 | MTR_7g015860 | 35.639 | 477 | 255 | 15 | 3 | 453 | 6 | 456 | 1.33e-77 | 249 |
| MS.gene44649.t1 | MTR_2g089670 | 37.277 | 448 | 250 | 16 | 22 | 453 | 41 | 473 | 4.07e-76 | 246 |
| MS.gene44649.t1 | MTR_6g015310 | 39.011 | 364 | 164 | 9 | 74 | 434 | 23 | 331 | 5.04e-76 | 241 |
| MS.gene44649.t1 | MTR_8g012750 | 34.647 | 482 | 275 | 14 | 3 | 457 | 4 | 472 | 8.75e-75 | 243 |
| MS.gene44649.t1 | MTR_3g087640 | 33.814 | 485 | 269 | 15 | 3 | 457 | 10 | 472 | 6.13e-69 | 227 |
| MS.gene44649.t1 | MTR_2g089650 | 32.848 | 481 | 274 | 15 | 3 | 459 | 10 | 465 | 8.91e-68 | 224 |
| MS.gene44649.t1 | MTR_2g089735 | 33.556 | 450 | 245 | 14 | 39 | 459 | 39 | 463 | 1.18e-66 | 221 |
| MS.gene44649.t1 | MTR_2g089755 | 32.456 | 456 | 271 | 14 | 26 | 459 | 37 | 477 | 4.06e-64 | 214 |
| MS.gene44649.t1 | MTR_7g014630 | 41.877 | 277 | 142 | 6 | 3 | 267 | 10 | 279 | 1.31e-62 | 207 |
| MS.gene44649.t1 | MTR_7g014205 | 77.273 | 132 | 18 | 2 | 295 | 426 | 1 | 120 | 2.27e-61 | 196 |
| MS.gene44649.t1 | MTR_7g014210 | 85.345 | 116 | 15 | 2 | 314 | 428 | 24 | 138 | 9.37e-59 | 191 |
| MS.gene44649.t1 | MTR_2g089665 | 31.448 | 442 | 185 | 11 | 1 | 432 | 1 | 334 | 3.77e-50 | 174 |
| MS.gene44649.t1 | MTR_7g014180 | 45.562 | 169 | 86 | 2 | 3 | 168 | 8 | 173 | 1.84e-41 | 145 |
| MS.gene44649.t1 | MTR_3g435300 | 39.914 | 233 | 114 | 7 | 3 | 223 | 4 | 222 | 4.97e-40 | 145 |
| MS.gene44649.t1 | MTR_7g014340 | 88.571 | 70 | 8 | 0 | 1 | 70 | 1 | 70 | 3.70e-36 | 129 |
| MS.gene44649.t1 | MTR_7g014670 | 36.649 | 191 | 114 | 5 | 272 | 457 | 71 | 259 | 2.28e-32 | 124 |
| MS.gene44649.t1 | MTR_7g014220 | 81.967 | 61 | 11 | 0 | 14 | 74 | 6 | 66 | 8.25e-27 | 103 |
| MS.gene44649.t1 | MTR_7g085930 | 26.698 | 427 | 268 | 14 | 57 | 457 | 54 | 461 | 5.82e-20 | 92.4 |
| MS.gene44649.t1 | MTR_0190s0020 | 28.668 | 443 | 236 | 23 | 23 | 438 | 26 | 415 | 7.34e-20 | 92.0 |
| MS.gene44649.t1 | MTR_3g026310 | 26.464 | 427 | 241 | 21 | 20 | 414 | 18 | 403 | 1.30e-19 | 91.3 |
| MS.gene44649.t1 | MTR_3g026950 | 25.734 | 443 | 253 | 18 | 32 | 434 | 21 | 427 | 2.04e-19 | 90.9 |
| MS.gene44649.t1 | MTR_8g075610 | 25.510 | 392 | 222 | 18 | 42 | 410 | 30 | 374 | 4.49e-19 | 89.4 |
| MS.gene44649.t1 | MTR_3g101900 | 24.819 | 415 | 252 | 13 | 20 | 413 | 24 | 399 | 4.99e-19 | 89.7 |
| MS.gene44649.t1 | MTR_4g094842 | 26.777 | 422 | 251 | 20 | 26 | 428 | 27 | 409 | 6.92e-19 | 89.0 |
| MS.gene44649.t1 | MTR_3g049150 | 25.980 | 408 | 233 | 17 | 34 | 410 | 30 | 399 | 1.07e-18 | 88.6 |
| MS.gene44649.t1 | MTR_2g064495 | 22.844 | 429 | 276 | 16 | 42 | 453 | 39 | 429 | 1.15e-18 | 88.2 |
| MS.gene44649.t1 | MTR_4g022825 | 25.962 | 416 | 249 | 16 | 20 | 414 | 18 | 395 | 2.67e-18 | 87.4 |
| MS.gene44649.t1 | MTR_7g026860 | 26.456 | 412 | 242 | 17 | 20 | 410 | 18 | 389 | 5.41e-18 | 86.3 |
| MS.gene44649.t1 | MTR_3g098980 | 25.245 | 408 | 250 | 15 | 62 | 454 | 66 | 433 | 8.20e-18 | 85.9 |
| MS.gene44649.t1 | MTR_3g101930 | 26.615 | 387 | 228 | 14 | 48 | 413 | 56 | 407 | 8.91e-18 | 85.9 |
| MS.gene44649.t1 | MTR_2g105330 | 22.844 | 429 | 276 | 16 | 42 | 453 | 39 | 429 | 1.11e-17 | 85.5 |
| MS.gene44649.t1 | MTR_7g085990 | 27.245 | 323 | 201 | 8 | 56 | 365 | 49 | 350 | 1.20e-17 | 85.5 |
| MS.gene44649.t1 | MTR_4g127650 | 24.569 | 464 | 284 | 19 | 24 | 457 | 29 | 456 | 8.05e-17 | 82.8 |
| MS.gene44649.t1 | MTR_3g088420 | 25.000 | 420 | 241 | 16 | 20 | 414 | 18 | 388 | 1.15e-16 | 82.4 |
| MS.gene44649.t1 | MTR_8g096530 | 24.507 | 355 | 233 | 11 | 62 | 411 | 59 | 383 | 1.21e-16 | 82.4 |
| MS.gene44649.t1 | MTR_7g086000 | 24.792 | 480 | 307 | 15 | 4 | 457 | 6 | 457 | 1.86e-16 | 82.0 |
| MS.gene44649.t1 | MTR_4g094835 | 22.983 | 409 | 254 | 18 | 26 | 414 | 32 | 399 | 5.00e-16 | 80.5 |
| MS.gene44649.t1 | MTR_8g079710 | 24.826 | 431 | 247 | 21 | 26 | 425 | 29 | 413 | 1.45e-15 | 79.0 |
| MS.gene44649.t1 | MTR_4g094828 | 24.138 | 406 | 271 | 14 | 20 | 414 | 23 | 402 | 1.48e-14 | 75.9 |
| MS.gene44649.t1 | MTR_4g022760 | 28.272 | 382 | 189 | 20 | 20 | 368 | 18 | 347 | 2.12e-13 | 72.0 |
| MS.gene44649.t1 | MTR_4g097090 | 24.818 | 411 | 252 | 14 | 24 | 409 | 28 | 406 | 4.41e-13 | 71.2 |
| MS.gene44649.t1 | MTR_7g027175 | 23.796 | 353 | 236 | 12 | 62 | 410 | 68 | 391 | 5.00e-13 | 71.2 |
| MS.gene44649.t1 | MTR_4g022290 | 24.020 | 408 | 263 | 14 | 20 | 414 | 24 | 397 | 2.72e-12 | 68.9 |
| MS.gene44649.t1 | MTR_3g465930 | 25.676 | 296 | 174 | 11 | 62 | 343 | 69 | 332 | 1.12e-11 | 67.0 |
| MS.gene44649.t1 | MTR_3g465950 | 25.356 | 351 | 225 | 14 | 62 | 401 | 68 | 392 | 2.44e-11 | 65.9 |
| MS.gene44649.t1 | MTR_4g094832 | 24.080 | 299 | 189 | 11 | 124 | 414 | 115 | 383 | 4.10e-11 | 65.1 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene44649.t1 | AT5G39090 | 38.865 | 458 | 257 | 11 | 5 | 457 | 8 | 447 | 3.93e-97 | 299 |
| MS.gene44649.t1 | AT3G29635 | 40.449 | 445 | 237 | 13 | 24 | 457 | 27 | 454 | 2.28e-94 | 293 |
| MS.gene44649.t1 | AT3G29670 | 38.330 | 467 | 257 | 13 | 4 | 459 | 5 | 451 | 1.06e-92 | 288 |
| MS.gene44649.t1 | AT5G39050 | 37.792 | 471 | 263 | 13 | 3 | 457 | 11 | 467 | 1.29e-91 | 286 |
| MS.gene44649.t1 | AT5G61160 | 37.580 | 471 | 258 | 13 | 3 | 459 | 4 | 452 | 8.22e-91 | 283 |
| MS.gene44649.t1 | AT5G39080 | 36.266 | 466 | 266 | 12 | 4 | 453 | 7 | 457 | 1.62e-90 | 283 |
| MS.gene44649.t1 | AT3G29590 | 39.103 | 468 | 242 | 14 | 4 | 457 | 10 | 448 | 1.42e-85 | 270 |
| MS.gene44649.t1 | AT3G29680 | 38.235 | 442 | 240 | 13 | 24 | 453 | 23 | 443 | 1.94e-81 | 259 |
| MS.gene44649.t1 | AT1G03940 | 34.874 | 476 | 269 | 14 | 3 | 457 | 9 | 464 | 7.11e-77 | 248 |
| MS.gene44649.t1 | AT1G03495 | 34.844 | 353 | 197 | 10 | 4 | 341 | 10 | 344 | 4.69e-54 | 184 |
| MS.gene44649.t1 | AT3G29720 | 36.458 | 192 | 102 | 5 | 99 | 286 | 26 | 201 | 2.41e-28 | 112 |
| MS.gene44649.t1 | AT2G19070 | 24.161 | 447 | 279 | 14 | 39 | 457 | 37 | 451 | 4.60e-22 | 99.0 |
| MS.gene44649.t1 | AT5G23940 | 25.635 | 433 | 271 | 15 | 26 | 437 | 25 | 427 | 3.71e-21 | 96.3 |
| MS.gene44649.t1 | AT5G48930 | 23.571 | 420 | 277 | 15 | 42 | 449 | 39 | 426 | 3.54e-18 | 87.0 |
| MS.gene44649.t1 | AT5G41040 | 24.444 | 360 | 227 | 13 | 62 | 410 | 69 | 394 | 4.27e-17 | 83.6 |
| MS.gene44649.t1 | AT5G41040 | 24.444 | 360 | 227 | 13 | 62 | 410 | 85 | 410 | 5.66e-17 | 83.6 |
| MS.gene44649.t1 | AT3G47170 | 26.408 | 284 | 170 | 11 | 62 | 329 | 70 | 330 | 9.47e-17 | 82.8 |
| MS.gene44649.t1 | AT3G29690 | 34.228 | 149 | 85 | 5 | 312 | 456 | 38 | 177 | 3.21e-16 | 77.0 |
| MS.gene44649.t1 | AT5G67150 | 25.768 | 423 | 258 | 16 | 10 | 413 | 13 | 398 | 6.30e-16 | 80.1 |
| MS.gene44649.t1 | AT5G67160 | 23.558 | 416 | 257 | 11 | 10 | 410 | 13 | 382 | 6.64e-15 | 77.0 |
| MS.gene44649.t1 | AT5G38130 | 25.537 | 419 | 247 | 16 | 29 | 426 | 52 | 426 | 1.00e-14 | 76.6 |
| MS.gene44649.t1 | AT3G48720 | 24.518 | 363 | 221 | 15 | 62 | 410 | 61 | 384 | 4.89e-14 | 74.3 |
| MS.gene44649.t1 | AT1G28680 | 22.071 | 367 | 232 | 10 | 62 | 413 | 62 | 389 | 1.46e-11 | 66.6 |
| MS.gene44649.t1 | AT1G28680 | 24.306 | 288 | 185 | 8 | 62 | 344 | 62 | 321 | 1.92e-11 | 65.9 |
Find 68 sgRNAs with CRISPR-Local
Find 98 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| ACGACTCCTGCAACATATTT+TGG | 0.108529 | 7.3:+83280198 | MS.gene44649:CDS |
| GCTGATTTCAATCATCTTTC+AGG | 0.150127 | 7.3:+83279559 | MS.gene44649:CDS |
| ATTGAAAATTTCTATGATTT+TGG | 0.163660 | 7.3:-83279242 | None:intergenic |
| TGTTGAAACAAGTGAGTTAT+TGG | 0.246021 | 7.3:+83280248 | MS.gene44649:CDS |
| GAAACCATTTCAACTTTCTT+TGG | 0.269632 | 7.3:-83280465 | None:intergenic |
| TTACTTTCTTTGACATACTA+TGG | 0.279036 | 7.3:+83279314 | MS.gene44649:CDS |
| TCATGATCAAGCTTCTGTTT+TGG | 0.280676 | 7.3:+83279639 | MS.gene44649:CDS |
| GTACTATAAACCTCAAATTT+AGG | 0.281913 | 7.3:-83280423 | None:intergenic |
| CCAATTGAAAATCCATAGTT+TGG | 0.297340 | 7.3:-83279682 | None:intergenic |
| AACCAACAATAGAAGCTTAA+TGG | 0.299594 | 7.3:+83279921 | MS.gene44649:CDS |
| GTTATTTCAATTATCTCGTT+TGG | 0.307673 | 7.3:+83279981 | MS.gene44649:CDS |
| GTTGAAACAAGTGAGTTATT+GGG | 0.310202 | 7.3:+83280249 | MS.gene44649:CDS |
| AGGCAAAATATTTGAGTGAT+TGG | 0.326856 | 7.3:+83279884 | MS.gene44649:CDS |
| ACAATAGAAGCTTAATGGTT+TGG | 0.327026 | 7.3:+83279926 | MS.gene44649:CDS |
| AACCATTAAGCTTCTATTGT+TGG | 0.335300 | 7.3:-83279923 | None:intergenic |
| TTTGGATATCGAAAAGCTTA+AGG | 0.345008 | 7.3:+83279999 | MS.gene44649:CDS |
| CAATAGAAGCTTAATGGTTT+GGG | 0.346870 | 7.3:+83279927 | MS.gene44649:CDS |
| TGTCTTTCTGATTGTAGAAA+TGG | 0.347109 | 7.3:+83280510 | MS.gene44649:CDS |
| GAGGTTTATAGTACTGATTT+TGG | 0.352559 | 7.3:+83280432 | MS.gene44649:CDS |
| TTGTATCCATAGCTTATGCC+TGG | 0.358494 | 7.3:+83280088 | MS.gene44649:CDS |
| TAGAAGAAAATTCTTTGAAC+AGG | 0.373178 | 7.3:-83279349 | None:intergenic |
| TGCTGGTTCACCTAAATTTG+AGG | 0.384622 | 7.3:+83280413 | MS.gene44649:CDS |
| CAAGGATCCAAATGAATTAG+AGG | 0.386216 | 7.3:+83279864 | MS.gene44649:CDS |
| TGAAGTGTTAAGTGAAGCTT+TGG | 0.391195 | 7.3:+83280296 | MS.gene44649:CDS |
| ATTTCTGATGTTAAAGCAAT+TGG | 0.393974 | 7.3:+83280387 | MS.gene44649:CDS |
| TCATGAGAAATATTCAAGTT+AGG | 0.396702 | 7.3:-83279622 | None:intergenic |
| ATTTGTAGTGTCAAAGCAAA+AGG | 0.403561 | 7.3:+83280023 | MS.gene44649:CDS |
| CAATTTCCAAAATATGTTGC+AGG | 0.404618 | 7.3:-83280204 | None:intergenic |
| CCTTTGATGAATTTAATGAT+TGG | 0.405006 | 7.3:-83279502 | None:intergenic |
| TTAAAAGATGGAGTGTTGAA+TGG | 0.421534 | 7.3:+83280324 | MS.gene44649:CDS |
| AAAACCAAAGAAAGTTGAAA+TGG | 0.434697 | 7.3:+83280461 | MS.gene44649:CDS |
| TTGCTTCGCTCTTTGTCAAA+GGG | 0.440215 | 7.3:+83280583 | MS.gene44649:CDS |
| AATGGTGATGGTGTTGAGAT+AGG | 0.443549 | 7.3:+83280528 | MS.gene44649:CDS |
| AGTATGTCAAAGAAAGTAAG+AGG | 0.444791 | 7.3:-83279310 | None:intergenic |
| GCTTTGGAAACTTTAAAAGA+TGG | 0.454178 | 7.3:+83280312 | MS.gene44649:CDS |
| TTTGCTTCGCTCTTTGTCAA+AGG | 0.460307 | 7.3:+83280582 | MS.gene44649:CDS |
| TTGAAACAAGTGAGTTATTG+GGG | 0.479171 | 7.3:+83280250 | MS.gene44649:CDS |
| ATGGTTTCTATAGATAGAAC+TGG | 0.485430 | 7.3:+83280480 | MS.gene44649:CDS |
| GTTAAAGCAATTGGTGCTGC+TGG | 0.491334 | 7.3:+83280396 | MS.gene44649:CDS |
| TGTCAAAGCAAAAGGGTACT+AGG | 0.499646 | 7.3:+83280031 | MS.gene44649:CDS |
| AAGAAAATTCTTTGAACAGG+TGG | 0.502827 | 7.3:-83279346 | None:intergenic |
| CCTATGTAGAAATCTTGAAA+AGG | 0.507641 | 7.3:+83279771 | MS.gene44649:CDS |
| GGTAGTTCTTCTTGTGAAGA+TGG | 0.511257 | 7.3:-83279271 | None:intergenic |
| AGCTTTCATTCTGCATACCC+AGG | 0.512729 | 7.3:-83280106 | None:intergenic |
| TGGCTTTGAATATTGATTGT+CGG | 0.525216 | 7.3:+83280157 | MS.gene44649:CDS |
| GTTTGGGAAGAAGGGTGTTG+AGG | 0.530313 | 7.3:-83279399 | None:intergenic |
| TCTACAATCAGAAAGACAAA+AGG | 0.535706 | 7.3:-83280505 | None:intergenic |
| GCATACCCAGGCATAAGCTA+TGG | 0.548203 | 7.3:-83280094 | None:intergenic |
| TGTATCCATAGCTTATGCCT+GGG | 0.550290 | 7.3:+83280089 | MS.gene44649:CDS |
| AAGGGTGTTGAGGAAGAGTG+AGG | 0.553924 | 7.3:-83279389 | None:intergenic |
| AGAGGAAGTGATGTATCTGA+AGG | 0.556506 | 7.3:-83279292 | None:intergenic |
| CCAATCATTAAATTCATCAA+AGG | 0.568697 | 7.3:+83279502 | MS.gene44649:CDS |
| TTTGTAGTGTCAAAGCAAAA+GGG | 0.581463 | 7.3:+83280024 | MS.gene44649:CDS |
| AGGAAGAGTGAGGCTTGGTG+TGG | 0.588661 | 7.3:-83279379 | None:intergenic |
| CAAATGAGATGACCCAAAAG+AGG | 0.588902 | 7.3:-83279460 | None:intergenic |
| TGTTGAGGAAGAGTGAGGCT+TGG | 0.589306 | 7.3:-83279384 | None:intergenic |
| TTGAGTGATTGGTCAAAGCA+AGG | 0.594084 | 7.3:+83279895 | MS.gene44649:CDS |
| ATCAGCATCAGATTCAGCTA+TGG | 0.602424 | 7.3:-83279542 | None:intergenic |
| AAATATGTTGCAGGAGTCGT+CGG | 0.603337 | 7.3:-83280195 | None:intergenic |
| GTATTTGTGTAGCTTCAGAA+AGG | 0.609581 | 7.3:-83279588 | None:intergenic |
| TCTGATTGTAGAAATGGTGA+TGG | 0.615354 | 7.3:+83280516 | MS.gene44649:CDS |
| GGAAGAGTGAGGCTTGGTGT+GGG | 0.646190 | 7.3:-83279378 | None:intergenic |
| TAGAAAGGACAAAACTCATG+TGG | 0.652604 | 7.3:-83279826 | None:intergenic |
| AGTGATTGGTCAAAGCAAGG+TGG | 0.675947 | 7.3:+83279898 | MS.gene44649:CDS |
| TGAAACAAGTGAGTTATTGG+GGG | 0.676029 | 7.3:+83280251 | MS.gene44649:CDS |
| TATGTTGCAGGAGTCGTCGG+TGG | 0.676124 | 7.3:-83280192 | None:intergenic |
| ATTGGCTTGTGAGAATCATG+AGG | 0.731339 | 7.3:-83279484 | None:intergenic |
| GGAAGATGGACTAATCGTGG+CGG | 0.752782 | 7.3:+83280272 | MS.gene44649:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | ATTATTTTTCTATCATAGAA+AGG | - | chr7.3:83279844-83279863 | None:intergenic | 15.0% |
| !!! | ATTGAAAATTTCTATGATTT+TGG | - | chr7.3:83279245-83279264 | None:intergenic | 15.0% |
| !! | CTATGATAGAAAAATAATCA+AGG | + | chr7.3:83279846-83279865 | MS.gene44649:CDS | 20.0% |
| !!! | AACTTCTTTTATCAAATCTT+GGG | + | chr7.3:83279744-83279763 | MS.gene44649:CDS | 20.0% |
| !!! | GAAAGTGATTTTTTTAGTTT+GGG | - | chr7.3:83279418-83279437 | None:intergenic | 20.0% |
| !!! | TGAAAGTGATTTTTTTAGTT+TGG | - | chr7.3:83279419-83279438 | None:intergenic | 20.0% |
| !!! | TTTCATTTTTATCCTCTTTT+GGG | + | chr7.3:83279448-83279467 | MS.gene44649:CDS | 20.0% |
| !!! | TTTTCATTTTTATCCTCTTT+TGG | + | chr7.3:83279447-83279466 | MS.gene44649:CDS | 20.0% |
| ! | AAAACCAAAGAAAGTTGAAA+TGG | + | chr7.3:83280461-83280480 | MS.gene44649:CDS | 25.0% |
| ! | AAATCCATAGTTTGGAAAAA+CGG | - | chr7.3:83279677-83279696 | None:intergenic | 25.0% |
| ! | ATTTCTGATGTTAAAGCAAT+TGG | + | chr7.3:83280387-83280406 | MS.gene44649:CDS | 25.0% |
| ! | CCAATCATTAAATTCATCAA+AGG | + | chr7.3:83279502-83279521 | MS.gene44649:CDS | 25.0% |
| ! | CCTTTGATGAATTTAATGAT+TGG | - | chr7.3:83279505-83279524 | None:intergenic | 25.0% |
| ! | GTACTATAAACCTCAAATTT+AGG | - | chr7.3:83280426-83280445 | None:intergenic | 25.0% |
| ! | TAGAAGAAAATTCTTTGAAC+AGG | - | chr7.3:83279352-83279371 | None:intergenic | 25.0% |
| ! | TTACTTTCTTTGACATACTA+TGG | + | chr7.3:83279314-83279333 | MS.gene44649:CDS | 25.0% |
| ! | TTCCAGAAAAATCATTTAGA+GGG | + | chr7.3:83279959-83279978 | MS.gene44649:CDS | 25.0% |
| !! | AATTTTTGTTCATCGTTACT+TGG | + | chr7.3:83280354-83280373 | MS.gene44649:CDS | 25.0% |
| !! | CAACTTCTTTTATCAAATCT+TGG | + | chr7.3:83279743-83279762 | MS.gene44649:CDS | 25.0% |
| !! | GTTATTTCAATTATCTCGTT+TGG | + | chr7.3:83279981-83280000 | MS.gene44649:CDS | 25.0% |
| !! | TATTTTGCCTCTAATTCATT+TGG | - | chr7.3:83279874-83279893 | None:intergenic | 25.0% |
| !! | TCATGAGAAATATTCAAGTT+AGG | - | chr7.3:83279625-83279644 | None:intergenic | 25.0% |
| !!! | ATTTTTTTAGTTTGGGAAGA+AGG | - | chr7.3:83279411-83279430 | None:intergenic | 25.0% |
| !!! | TATTTTGGAAATTGTATTGC+AGG | + | chr7.3:83280213-83280232 | MS.gene44649:CDS | 25.0% |
| !!! | TTTTTTTAGTTTGGGAAGAA+GGG | - | chr7.3:83279410-83279429 | None:intergenic | 25.0% |
| AAACTCATGTGGCAAAAAAA+TGG | - | chr7.3:83279818-83279837 | None:intergenic | 30.0% | |
| AACCAACAATAGAAGCTTAA+TGG | + | chr7.3:83279921-83279940 | MS.gene44649:CDS | 30.0% | |
| AACCATTAAGCTTCTATTGT+TGG | - | chr7.3:83279926-83279945 | None:intergenic | 30.0% | |
| AACTCATGTGGCAAAAAAAT+GGG | - | chr7.3:83279817-83279836 | None:intergenic | 30.0% | |
| AAGAAAATTCTTTGAACAGG+TGG | - | chr7.3:83279349-83279368 | None:intergenic | 30.0% | |
| ACAATAGAAGCTTAATGGTT+TGG | + | chr7.3:83279926-83279945 | MS.gene44649:CDS | 30.0% | |
| AGGCAAAATATTTGAGTGAT+TGG | + | chr7.3:83279884-83279903 | MS.gene44649:CDS | 30.0% | |
| AGTATGTCAAAGAAAGTAAG+AGG | - | chr7.3:83279313-83279332 | None:intergenic | 30.0% | |
| ATGGTTTCTATAGATAGAAC+TGG | + | chr7.3:83280480-83280499 | MS.gene44649:CDS | 30.0% | |
| ATTTGTAGTGTCAAAGCAAA+AGG | + | chr7.3:83280023-83280042 | MS.gene44649:CDS | 30.0% | |
| CAATAGAAGCTTAATGGTTT+GGG | + | chr7.3:83279927-83279946 | MS.gene44649:CDS | 30.0% | |
| CAATTTCCAAAATATGTTGC+AGG | - | chr7.3:83280207-83280226 | None:intergenic | 30.0% | |
| CCAATTGAAAATCCATAGTT+TGG | - | chr7.3:83279685-83279704 | None:intergenic | 30.0% | |
| CCTATGTAGAAATCTTGAAA+AGG | + | chr7.3:83279771-83279790 | MS.gene44649:CDS | 30.0% | |
| GAAACCATTTCAACTTTCTT+TGG | - | chr7.3:83280468-83280487 | None:intergenic | 30.0% | |
| GTTCCAGAAAAATCATTTAG+AGG | + | chr7.3:83279958-83279977 | MS.gene44649:CDS | 30.0% | |
| GTTGAAACAAGTGAGTTATT+GGG | + | chr7.3:83280249-83280268 | MS.gene44649:CDS | 30.0% | |
| TAAGAACAAAAATGCTCTGA+TGG | + | chr7.3:83280137-83280156 | MS.gene44649:CDS | 30.0% | |
| TCTACAATCAGAAAGACAAA+AGG | - | chr7.3:83280508-83280527 | None:intergenic | 30.0% | |
| TGTCTTTCTGATTGTAGAAA+TGG | + | chr7.3:83280510-83280529 | MS.gene44649:CDS | 30.0% | |
| TGTGTCTAACAAAAAAGCAA+TGG | + | chr7.3:83280554-83280573 | MS.gene44649:CDS | 30.0% | |
| TGTTGAAACAAGTGAGTTAT+TGG | + | chr7.3:83280248-83280267 | MS.gene44649:CDS | 30.0% | |
| TTGAAACAAGTGAGTTATTG+GGG | + | chr7.3:83280250-83280269 | MS.gene44649:CDS | 30.0% | |
| TTTGGATATCGAAAAGCTTA+AGG | + | chr7.3:83279999-83280018 | MS.gene44649:CDS | 30.0% | |
| TTTGTAGTGTCAAAGCAAAA+GGG | + | chr7.3:83280024-83280043 | MS.gene44649:CDS | 30.0% | |
| ! | AACCCTCTAAATGATTTTTC+TGG | - | chr7.3:83279964-83279983 | None:intergenic | 30.0% |
| ! | CCAAACTATGGATTTTCAAT+TGG | + | chr7.3:83279682-83279701 | MS.gene44649:CDS | 30.0% |
| !! | CCTTTTCAAGATTTCTACAT+AGG | - | chr7.3:83279774-83279793 | None:intergenic | 30.0% |
| !! | GCTTTGGAAACTTTAAAAGA+TGG | + | chr7.3:83280312-83280331 | MS.gene44649:CDS | 30.0% |
| !! | TGGCTTTGAATATTGATTGT+CGG | + | chr7.3:83280157-83280176 | MS.gene44649:CDS | 30.0% |
| !! | TTAAAAGATGGAGTGTTGAA+TGG | + | chr7.3:83280324-83280343 | MS.gene44649:CDS | 30.0% |
| !!! | GAGGTTTATAGTACTGATTT+TGG | + | chr7.3:83280432-83280451 | MS.gene44649:CDS | 30.0% |
| !!! | TATAGTACTGATTTTGGTTG+TGG | + | chr7.3:83280438-83280457 | MS.gene44649:CDS | 30.0% |
| CAAGGATCCAAATGAATTAG+AGG | + | chr7.3:83279864-83279883 | MS.gene44649:CDS | 35.0% | |
| GTATTTGTGTAGCTTCAGAA+AGG | - | chr7.3:83279591-83279610 | None:intergenic | 35.0% | |
| TAGAAAGGACAAAACTCATG+TGG | - | chr7.3:83279829-83279848 | None:intergenic | 35.0% | |
| TCTGATTGTAGAAATGGTGA+TGG | + | chr7.3:83280516-83280535 | MS.gene44649:CDS | 35.0% | |
| TGAAACAAGTGAGTTATTGG+GGG | + | chr7.3:83280251-83280270 | MS.gene44649:CDS | 35.0% | |
| ! | GCTGATTTCAATCATCTTTC+AGG | + | chr7.3:83279559-83279578 | MS.gene44649:CDS | 35.0% |
| ! | TCATGATCAAGCTTCTGTTT+TGG | + | chr7.3:83279639-83279658 | MS.gene44649:CDS | 35.0% |
| ! | TGAAGTGTTAAGTGAAGCTT+TGG | + | chr7.3:83280296-83280315 | MS.gene44649:CDS | 35.0% |
| ! | TTTTGTTCATCGTTACTTGG+CGG | + | chr7.3:83280357-83280376 | MS.gene44649:CDS | 35.0% |
| !! | GTAACCGTTTTTCCAAACTA+TGG | + | chr7.3:83279670-83279689 | MS.gene44649:CDS | 35.0% |
| !!! | TCACATCATGCTGTTTTAGA+TGG | + | chr7.3:83279712-83279731 | MS.gene44649:CDS | 35.0% |
| AAATATGTTGCAGGAGTCGT+CGG | - | chr7.3:83280198-83280217 | None:intergenic | 40.0% | |
| AGAGGAAGTGATGTATCTGA+AGG | - | chr7.3:83279295-83279314 | None:intergenic | 40.0% | |
| ATCAGCATCAGATTCAGCTA+TGG | - | chr7.3:83279545-83279564 | None:intergenic | 40.0% | |
| CAAATGAGATGACCCAAAAG+AGG | - | chr7.3:83279463-83279482 | None:intergenic | 40.0% | |
| GGTAGTTCTTCTTGTGAAGA+TGG | - | chr7.3:83279274-83279293 | None:intergenic | 40.0% | |
| TGTATCCATAGCTTATGCCT+GGG | + | chr7.3:83280089-83280108 | MS.gene44649:CDS | 40.0% | |
| TTGCTTCGCTCTTTGTCAAA+GGG | + | chr7.3:83280583-83280602 | MS.gene44649:CDS | 40.0% | |
| TTGTATCCATAGCTTATGCC+TGG | + | chr7.3:83280088-83280107 | MS.gene44649:CDS | 40.0% | |
| TTTGCTTCGCTCTTTGTCAA+AGG | + | chr7.3:83280582-83280601 | MS.gene44649:CDS | 40.0% | |
| ! | ACGACTCCTGCAACATATTT+TGG | + | chr7.3:83280198-83280217 | MS.gene44649:CDS | 40.0% |
| ! | TTGAGTGATTGGTCAAAGCA+AGG | + | chr7.3:83279895-83279914 | MS.gene44649:CDS | 40.0% |
| !! | AATGGTGATGGTGTTGAGAT+AGG | + | chr7.3:83280528-83280547 | MS.gene44649:CDS | 40.0% |
| !! | ATTGGCTTGTGAGAATCATG+AGG | - | chr7.3:83279487-83279506 | None:intergenic | 40.0% |
| !! | CTCTTTTGGGTCATCTCATT+TGG | + | chr7.3:83279461-83279480 | MS.gene44649:CDS | 40.0% |
| !! | TGCTGGTTCACCTAAATTTG+AGG | + | chr7.3:83280413-83280432 | MS.gene44649:CDS | 40.0% |
| !! | TGTCAAAGCAAAAGGGTACT+AGG | + | chr7.3:83280031-83280050 | MS.gene44649:CDS | 40.0% |
| AGCTTTCATTCTGCATACCC+AGG | - | chr7.3:83280109-83280128 | None:intergenic | 45.0% | |
| AGTGAGTTATTGGGGGAAGA+TGG | + | chr7.3:83280258-83280277 | MS.gene44649:CDS | 45.0% | |
| ! | AGTGATTGGTCAAAGCAAGG+TGG | + | chr7.3:83279898-83279917 | MS.gene44649:CDS | 45.0% |
| !! | GTTAAAGCAATTGGTGCTGC+TGG | + | chr7.3:83280396-83280415 | MS.gene44649:CDS | 45.0% |
| GCATACCCAGGCATAAGCTA+TGG | - | chr7.3:83280097-83280116 | None:intergenic | 50.0% | |
| TGTTGAGGAAGAGTGAGGCT+TGG | - | chr7.3:83279387-83279406 | None:intergenic | 50.0% | |
| !! | AAGGGTGTTGAGGAAGAGTG+AGG | - | chr7.3:83279392-83279411 | None:intergenic | 50.0% |
| !! | GGAAGATGGACTAATCGTGG+CGG | + | chr7.3:83280272-83280291 | MS.gene44649:CDS | 50.0% |
| !! | GTTTGGGAAGAAGGGTGTTG+AGG | - | chr7.3:83279402-83279421 | None:intergenic | 50.0% |
| TATGTTGCAGGAGTCGTCGG+TGG | - | chr7.3:83280195-83280214 | None:intergenic | 55.0% | |
| !! | AGGAAGAGTGAGGCTTGGTG+TGG | - | chr7.3:83279382-83279401 | None:intergenic | 55.0% |
| !! | GGAAGAGTGAGGCTTGGTGT+GGG | - | chr7.3:83279381-83279400 | None:intergenic | 55.0% |
| !! | GGGGGAAGATGGACTAATCG+TGG | + | chr7.3:83280269-83280288 | MS.gene44649:CDS | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr7.3 | gene | 83279238 | 83280617 | 83279238 | ID=MS.gene44649 |
| chr7.3 | mRNA | 83279238 | 83280617 | 83279238 | ID=MS.gene44649.t1;Parent=MS.gene44649 |
| chr7.3 | exon | 83279238 | 83280617 | 83279238 | ID=MS.gene44649.t1.exon1;Parent=MS.gene44649.t1 |
| chr7.3 | CDS | 83279238 | 83280617 | 83279238 | ID=cds.MS.gene44649.t1;Parent=MS.gene44649.t1 |
| Gene Sequence |
| Protein sequence |
