AlfalfaGEDB | MS.gene56053

M. sativa cultivar XinJiangDaYe / MS.gene56053

KEGG: -
GO: -
PFAM: PF03181.18

Nr:

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene56053.t1	XP_003588551.1	96.2	236	9	0	31	266	150	385	4.20E-127	464.2

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Swissprot:

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene56053.t1	Q08298	58.5	241	96	4	28	265	152	391	1.4e-75	284.3

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Trembl:

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene56053.t1	G7I4D4	96.2	236	9	0	31	266	150	385	3.0e-127	464.2

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TFs/TRs:

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Gene ID	Type	Classification
No data available in table

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Protein Kinases:

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Gene ID	Type	Classification
No data available in table

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Network:

Co-expression Network:

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Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene049150	MS.gene56053	0.853432	2.40E-61	-1.69E-46
MS.gene049151	MS.gene56053	0.83569	1.45E-56	-1.69E-46
MS.gene049153	MS.gene56053	0.846588	1.98E-59	-1.69E-46
MS.gene049233	MS.gene56053	0.828474	8.81E-55	-1.69E-46
MS.gene049260	MS.gene56053	0.826127	3.22E-54	-1.69E-46
MS.gene049378	MS.gene56053	0.83021	3.34E-55	-1.69E-46
MS.gene049382	MS.gene56053	0.806927	6.48E-50	-1.69E-46
MS.gene049758	MS.gene56053	0.831872	1.30E-55	-1.69E-46
MS.gene049910	MS.gene56053	0.806801	6.89E-50	-1.69E-46
MS.gene049915	MS.gene56053	0.800337	1.51E-48	-1.69E-46

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PPI:

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Gene1	Gene2	Type
No data available in table

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene56053.t1	MTR_1g008500	82.000	300	20	3	1	266	86	385	3.78e-174	485
MS.gene56053.t1	MTR_1g008590	76.245	261	52	3	6	266	75	325	6.72e-144	406
MS.gene56053.t1	MTR_0312s0030	75.188	266	56	3	1	266	70	325	9.53e-144	406
MS.gene56053.t1	MTR_1g007520	75.188	266	56	3	1	266	70	325	9.53e-144	406
MS.gene56053.t1	MTR_1g008510	79.832	238	43	2	31	266	92	326	2.60e-139	395
MS.gene56053.t1	MTR_1g008560	72.761	268	66	3	3	266	66	330	7.41e-137	389
MS.gene56053.t1	MTR_1g008530	78.539	219	46	1	49	266	6	224	4.14e-129	365
MS.gene56053.t1	MTR_1g008470	74.468	235	57	3	32	266	69	300	1.66e-127	364
MS.gene56053.t1	MTR_1g008450	73.504	234	59	3	33	266	83	313	1.10e-121	350
MS.gene56053.t1	MTR_1g008460	64.639	263	84	4	4	266	44	297	2.49e-117	338

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene56053.t1	AT5G25610	58.506	241	96	4	28	265	152	391	1.62e-98	294
MS.gene56053.t1	AT1G49320	37.662	231	134	5	45	266	51	280	1.53e-50	167
MS.gene56053.t1	AT1G60390	35.780	218	128	6	52	263	409	620	6.82e-37	138
MS.gene56053.t1	AT1G70370	35.023	217	133	4	52	264	411	623	5.42e-36	135
MS.gene56053.t1	AT1G70370	35.023	217	133	4	52	264	411	623	5.42e-36	135
MS.gene56053.t1	AT1G23760	34.101	217	135	5	52	264	407	619	8.35e-34	129

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Find 38 sgRNAs with CRISPR-Local

Find 47 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAACAACATGATCCTGTGGA+AGG	0.641220	1.3:+11384279	None:intergenic
AAAGATATTAAAGGACAACC+AGG	0.645079	1.3:-11384989	MS.gene56053:CDS
AAAGCTTTGCGTGACATCAT+TGG	0.419403	1.3:-11384654	MS.gene56053:CDS
AAAGGACAACCAGGAAAAGG+TGG	0.597740	1.3:-11384980	MS.gene56053:CDS
AACATGTTCTCCATCAAGGA+AGG	0.601008	1.3:-11384746	MS.gene56053:CDS
AACTATATGAATGTTGATGC+AGG	0.499341	1.3:-11385049	None:intergenic
AAGAAGAAAAGTGCTACATT+TGG	0.370145	1.3:+11384905	None:intergenic
ACAATGGAATCCTAAACATT+TGG	0.370419	1.3:-11384351	MS.gene56053:CDS
ACCCAAACAACATGATCCTG+TGG	0.647573	1.3:+11384275	None:intergenic
ACTTGAAAAGCCAAATGTTT+AGG	0.303666	1.3:+11384341	None:intergenic

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
AAAGATATTAAAGGACAACC+AGG	-	chr1.3:11384319-11384338	MS.gene56053:CDS	30.0%
ACAATGGAATCCTAAACATT+TGG	-	chr1.3:11384957-11384976	MS.gene56053:CDS	30.0%
ACTTGAAAAGCCAAATGTTT+AGG	+	chr1.3:11384970-11384989	None:intergenic	30.0%
ATAAGTGAGTGTGAAGAAAA+TGG	-	chr1.3:11384616-11384635	MS.gene56053:CDS	30.0%
CAAGAATATGTAATGGCAAA+AGG	-	chr1.3:11384763-11384782	MS.gene56053:CDS	30.0%
GCAGCATAATTGTAAAACAT+AGG	+	chr1.3:11384358-11384377	None:intergenic	30.0%
TGTACTTTGCTTGAAGAAAA+AGG	+	chr1.3:11384532-11384551	None:intergenic	30.0%
ATGCAGGAAAAACAAAGTCA+AGG	-	chr1.3:11384275-11384294	MS.gene56053:CDS	35.0%
ATTAAAGGACAACCAGGAAA+AGG	-	chr1.3:11384325-11384344	MS.gene56053:CDS	35.0%
GATTTCACTACTTCGAAACT+TGG	-	chr1.3:11384688-11384707	MS.gene56053:CDS	35.0%

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Chromosome	Type	Strat	End	Strand	Name
chr1.3	gene	11384265	11385065	11384265	ID=MS.gene56053
chr1.3	mRNA	11384265	11385065	11384265	ID=MS.gene56053.t1;Parent=MS.gene56053
chr1.3	exon	11384265	11385065	11384265	ID=MS.gene56053.t1.exon1;Parent=MS.gene56053.t1
chr1.3	CDS	11384265	11385065	11384265	ID=cds.MS.gene56053.t1;Parent=MS.gene56053.t1

Gene Sequence

>MS.gene56053

ATGAATGTTGATGCAGGAAAAACAAAGTCAAGGGGAACATCAGTCAACATAGAAAAAGATATTAAAGGACAACCAGGAAAAGGTGGTTTTCCTATGTTTTACAATTATGCTGCAAGTGAGACACAATTACATGATAAACCAAATGTAGCACTTTTCTTCTTAGAAAAGGATTTACATCAAGGAACAAAGTTGAACTTGCAATTCACAAAAACCAACTCAAATGATGATGCAAAATTCTTACCTAAAGAAGTTTCTAATTCAATACCTTTTTCTTCAAGCAAAGTACAAAACATCCTTAACATGTTCTCCATCAAGGAAGGAACAGAAGAATCTGAAATTGTGAAGAACACAATAAGTGAGTGTGAAGAAAATGGCATCAAAGGAGAGGAAAAGCTTTGCGTGACATCATTGGAATCTATGGTTGATTTCACTACTTCGAAACTTGGAAACAATGTTGAAGCCGTTTCAACAGAAGTGAAAAAAGAAAGTAGTGACTTGCAAGAATATGTAATGGCAAAAGGTGTGAAGAAATTGGGAGAGAAGAATAAAGCTGTAGTGTGTCACAAAGAGAATTACCCTTATGCAATTTTTTACTGTCATAAAACAGATACAACTAAAGTTTACTCTGTGCCTTTGGAAGGTGTTGATGGAAGTAGAGTTAAAGCTGTTGCTGTGTGTCACACTGATACTTCACAATGGAATCCTAAACATTTGGCTTTTCAAGTGCTCAAAGTTCAACCTGGAACTGTTCCAGTTTGTCACTTCCTTCCACAGGATCATGTTGTTTGGGTTTCCAAGTAA

Protein sequence

>MS.gene56053.t1

MNVDAGKTKSRGTSVNIEKDIKGQPGKGGFPMFYNYAASETQLHDKPNVALFFLEKDLHQGTKLNLQFTKTNSNDDAKFLPKEVSNSIPFSSSKVQNILNMFSIKEGTEESEIVKNTISECEENGIKGEEKLCVTSLESMVDFTTSKLGNNVEAVSTEVKKESSDLQEYVMAKGVKKLGEKNKAVVCHKENYPYAIFYCHKTDTTKVYSVPLEGVDGSRVKAVAVCHTDTSQWNPKHLAFQVLKVQPGTVPVCHFLPQDHVVWVSK