AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene56053


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene56053.t1 MTR_1g008500 82.000 300 20 3 1 266 86 385 3.78e-174 485
MS.gene56053.t1 MTR_1g008590 76.245 261 52 3 6 266 75 325 6.72e-144 406
MS.gene56053.t1 MTR_0312s0030 75.188 266 56 3 1 266 70 325 9.53e-144 406
MS.gene56053.t1 MTR_1g007520 75.188 266 56 3 1 266 70 325 9.53e-144 406
MS.gene56053.t1 MTR_1g008510 79.832 238 43 2 31 266 92 326 2.60e-139 395
MS.gene56053.t1 MTR_1g008560 72.761 268 66 3 3 266 66 330 7.41e-137 389
MS.gene56053.t1 MTR_1g008530 78.539 219 46 1 49 266 6 224 4.14e-129 365
MS.gene56053.t1 MTR_1g008470 74.468 235 57 3 32 266 69 300 1.66e-127 364
MS.gene56053.t1 MTR_1g008450 73.504 234 59 3 33 266 83 313 1.10e-121 350
MS.gene56053.t1 MTR_1g008460 64.639 263 84 4 4 266 44 297 2.49e-117 338
MS.gene56053.t1 MTR_3g109490 57.407 270 102 5 2 264 57 320 1.44e-108 317
MS.gene56053.t1 MTR_1g008580 82.456 171 30 0 95 265 1 171 8.13e-106 304
MS.gene56053.t1 MTR_4g069680 41.518 224 126 4 47 266 65 287 1.54e-60 193
MS.gene56053.t1 MTR_3g107830 42.411 224 124 3 47 266 34 256 4.99e-60 191
MS.gene56053.t1 MTR_3g116410 41.964 224 125 4 47 266 54 276 1.12e-58 188
MS.gene56053.t1 MTR_3g116380 43.049 223 119 5 47 264 82 301 3.91e-58 187
MS.gene56053.t1 MTR_3g116320 40.826 218 119 5 52 264 474 686 1.80e-47 167
MS.gene56053.t1 MTR_3g116270 38.532 218 124 5 52 264 292 504 7.56e-47 163
MS.gene56053.t1 MTR_4g069570 37.900 219 126 6 52 264 120 334 6.26e-46 157
MS.gene56053.t1 MTR_4g069040 36.709 237 139 7 34 264 277 508 6.66e-46 161
MS.gene56053.t1 MTR_4g069550 37.900 219 126 6 52 264 247 461 8.69e-43 152
MS.gene56053.t1 MTR_4g069520 37.443 219 127 6 52 264 507 721 2.18e-41 151
MS.gene56053.t1 MTR_4g069520 37.443 219 127 6 52 264 493 707 2.61e-41 150
MS.gene56053.t1 MTR_8g064500 36.530 219 129 5 52 265 159 372 4.83e-41 145
MS.gene56053.t1 MTR_8g064560 35.616 219 131 5 52 265 412 625 8.75e-38 140
MS.gene56053.t1 MTR_8g064530 35.160 219 132 6 52 265 429 642 5.47e-36 135
MS.gene56053.t1 MTR_5g034320 33.641 217 136 5 52 264 415 627 8.50e-36 135
MS.gene56053.t1 MTR_3g078090 37.696 191 112 4 79 265 439 626 1.22e-35 134
MS.gene56053.t1 MTR_8g445170 42.000 150 80 3 115 264 240 382 8.62e-35 129
MS.gene56053.t1 MTR_6g086530 33.846 195 121 6 79 266 13 206 4.26e-34 122
MS.gene56053.t1 MTR_8g046000 35.909 220 107 8 51 264 30 221 5.23e-34 123
MS.gene56053.t1 MTR_8g445140 41.667 144 77 3 121 264 165 301 1.67e-33 124
MS.gene56053.t1 MTR_2g081610 35.514 214 109 7 52 264 73 258 5.22e-33 121
MS.gene56053.t1 MTR_8g045890 44.444 144 73 4 121 264 294 430 6.06e-33 125
MS.gene56053.t1 MTR_8g045890 44.444 144 73 4 121 264 335 471 1.23e-32 124
MS.gene56053.t1 MTR_8g045890 44.444 144 73 4 121 264 372 508 1.57e-32 125
MS.gene56053.t1 MTR_1g008420 74.390 82 19 2 38 118 119 199 1.76e-31 116
MS.gene56053.t1 MTR_8g045880 42.466 146 77 3 119 264 175 313 2.69e-31 118
MS.gene56053.t1 MTR_4g133840 34.043 188 105 4 79 263 15 186 2.86e-31 115
MS.gene56053.t1 MTR_8g045880 42.466 146 77 3 119 264 210 348 4.85e-31 118
MS.gene56053.t1 MTR_8g045880 42.466 146 77 3 119 264 212 350 5.44e-31 118
MS.gene56053.t1 MTR_2g081590 33.645 214 113 7 52 264 73 258 1.57e-30 115
MS.gene56053.t1 MTR_2g081770 31.718 227 105 6 52 264 79 269 3.21e-30 114
MS.gene56053.t1 MTR_5g033340 30.455 220 145 5 50 265 164 379 3.66e-27 108
MS.gene56053.t1 MTR_5g033340 31.544 149 96 3 102 247 8 153 1.05e-15 76.3
MS.gene56053.t1 MTR_1g045860 32.110 218 115 7 52 264 69 258 3.87e-27 106
MS.gene56053.t1 MTR_5g033350 31.250 192 125 4 78 265 12 200 9.55e-26 100
MS.gene56053.t1 MTR_8g044290 44.545 110 55 3 121 230 229 332 8.16e-24 100
MS.gene56053.t1 MTR_5g033330 29.817 218 145 5 50 263 403 616 8.92e-24 100
MS.gene56053.t1 MTR_5g033330 30.198 202 133 5 50 247 195 392 1.25e-20 91.3
MS.gene56053.t1 MTR_5g033330 31.034 174 113 4 78 247 14 184 7.67e-18 83.2
MS.gene56053.t1 MTR_4g068420 35.294 136 68 5 132 264 1 119 6.46e-22 88.6
MS.gene56053.t1 MTR_5g033360 28.311 219 133 6 50 265 25 222 7.56e-21 88.6
MS.gene56053.t1 MTR_8g075930 40.506 79 43 2 179 254 10 87 3.03e-18 77.8
MS.gene56053.t1 MTR_5g033430 33.333 87 56 2 181 265 24 110 2.10e-13 65.9
MS.gene56053.t1 MTR_5g033440 32.184 87 57 2 181 265 24 110 9.00e-13 65.9
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene56053.t1 AT5G25610 58.506 241 96 4 28 265 152 391 1.62e-98 294
MS.gene56053.t1 AT1G49320 37.662 231 134 5 45 266 51 280 1.53e-50 167
MS.gene56053.t1 AT1G60390 35.780 218 128 6 52 263 409 620 6.82e-37 138
MS.gene56053.t1 AT1G70370 35.023 217 133 4 52 264 411 623 5.42e-36 135
MS.gene56053.t1 AT1G70370 35.023 217 133 4 52 264 411 623 5.42e-36 135
MS.gene56053.t1 AT1G23760 34.101 217 135 5 52 264 407 619 8.35e-34 129

Find 38 sgRNAs with CRISPR-Local

Find 47 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTCTTCTGTTCCTTCCTTGA+TGG 0.248730 1.3:+11384736 None:intergenic
GGCAAAAGGTGTGAAGAAAT+TGG 0.273962 1.3:-11384531 MS.gene56053:CDS
TGTACTTTGCTTGAAGAAAA+AGG 0.274463 1.3:+11384779 None:intergenic
TTCCACAGGATCATGTTGTT+TGG 0.285326 1.3:-11384277 MS.gene56053:CDS
ACTTGAAAAGCCAAATGTTT+AGG 0.303666 1.3:+11384341 None:intergenic
CGTGACATCATTGGAATCTA+TGG 0.304272 1.3:-11384645 MS.gene56053:CDS
TCCACAGGATCATGTTGTTT+GGG 0.305699 1.3:-11384276 MS.gene56053:CDS
ATTGAATTAGAAACTTCTTT+AGG 0.310804 1.3:+11384803 None:intergenic
ATAAGTGAGTGTGAAGAAAA+TGG 0.348496 1.3:-11384692 MS.gene56053:CDS
GTGCCTTTGGAAGGTGTTGA+TGG 0.355685 1.3:-11384416 MS.gene56053:CDS
AAGAAGAAAAGTGCTACATT+TGG 0.370145 1.3:+11384905 None:intergenic
ACAATGGAATCCTAAACATT+TGG 0.370419 1.3:-11384351 MS.gene56053:CDS
TAAAGTTTACTCTGTGCCTT+TGG 0.379443 1.3:-11384429 MS.gene56053:CDS
TGACAAACTGGAACAGTTCC+AGG 0.395778 1.3:+11384305 None:intergenic
AAAGCTTTGCGTGACATCAT+TGG 0.419403 1.3:-11384654 MS.gene56053:CDS
GCAAAAGGTGTGAAGAAATT+GGG 0.438714 1.3:-11384530 MS.gene56053:CDS
CAAGAATATGTAATGGCAAA+AGG 0.445313 1.3:-11384545 MS.gene56053:CDS
CCTTGATGGAGAACATGTTA+AGG 0.448883 1.3:+11384750 None:intergenic
TGTGGAAGGAAGTGACAAAC+TGG 0.450964 1.3:+11384293 None:intergenic
GATTTCACTACTTCGAAACT+TGG 0.458312 1.3:-11384620 MS.gene56053:CDS
TTAGAAAAGGATTTACATCA+AGG 0.470233 1.3:-11384884 MS.gene56053:CDS
CAAGTGCTCAAAGTTCAACC+TGG 0.487263 1.3:-11384323 MS.gene56053:CDS
AACTATATGAATGTTGATGC+AGG 0.499341 1.3:-11385049 None:intergenic
AGTTTGTCACTTCCTTCCAC+AGG 0.501409 1.3:-11384291 MS.gene56053:CDS
ATTAAAGGACAACCAGGAAA+AGG 0.504978 1.3:-11384983 MS.gene56053:CDS
GTTTACTCTGTGCCTTTGGA+AGG 0.519063 1.3:-11384425 MS.gene56053:CDS
TGTGAAGAAAATGGCATCAA+AGG 0.529052 1.3:-11384683 MS.gene56053:CDS
CCTTAACATGTTCTCCATCA+AGG 0.536982 1.3:-11384750 MS.gene56053:CDS
TGACTTGCAAGAATATGTAA+TGG 0.566257 1.3:-11384552 MS.gene56053:CDS
GCAGCATAATTGTAAAACAT+AGG 0.586082 1.3:+11384953 None:intergenic
AAAGGACAACCAGGAAAAGG+TGG 0.597740 1.3:-11384980 MS.gene56053:CDS
AACATGTTCTCCATCAAGGA+AGG 0.601008 1.3:-11384746 MS.gene56053:CDS
AAACAACATGATCCTGTGGA+AGG 0.641220 1.3:+11384279 None:intergenic
AGAAAATGGCATCAAAGGAG+AGG 0.642414 1.3:-11384678 MS.gene56053:CDS
AAAGATATTAAAGGACAACC+AGG 0.645079 1.3:-11384989 MS.gene56053:CDS
ACCCAAACAACATGATCCTG+TGG 0.647573 1.3:+11384275 None:intergenic
CTTCCATCAACACCTTCCAA+AGG 0.662188 1.3:+11384413 None:intergenic
GTCACACTGATACTTCACAA+TGG 0.703629 1.3:-11384367 MS.gene56053:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AACATAGAAAAAGATATTAA+AGG - chr1.3:11384310-11384329 MS.gene56053:CDS 15.0%
!! ATTGAATTAGAAACTTCTTT+AGG + chr1.3:11384508-11384527 None:intergenic 20.0%
! GACAGTAAAAAATTGCATAA+GGG + chr1.3:11384843-11384862 None:intergenic 25.0%
! TGACAGTAAAAAATTGCATA+AGG + chr1.3:11384844-11384863 None:intergenic 25.0%
! TTAGAAAAGGATTTACATCA+AGG - chr1.3:11384424-11384443 MS.gene56053:CDS 25.0%
!!! TTTTTTCACTTCTGTTGAAA+CGG + chr1.3:11384728-11384747 None:intergenic 25.0%
AAAGATATTAAAGGACAACC+AGG - chr1.3:11384319-11384338 MS.gene56053:CDS 30.0%
ACAATGGAATCCTAAACATT+TGG - chr1.3:11384957-11384976 MS.gene56053:CDS 30.0%
ACTTGAAAAGCCAAATGTTT+AGG + chr1.3:11384970-11384989 None:intergenic 30.0%
ATAAGTGAGTGTGAAGAAAA+TGG - chr1.3:11384616-11384635 MS.gene56053:CDS 30.0%
CAAGAATATGTAATGGCAAA+AGG - chr1.3:11384763-11384782 MS.gene56053:CDS 30.0%
GCAGCATAATTGTAAAACAT+AGG + chr1.3:11384358-11384377 None:intergenic 30.0%
TGTACTTTGCTTGAAGAAAA+AGG + chr1.3:11384532-11384551 None:intergenic 30.0%
! AGCACTTTTCTTCTTAGAAA+AGG - chr1.3:11384411-11384430 MS.gene56053:CDS 30.0%
! TGACTTGCAAGAATATGTAA+TGG - chr1.3:11384756-11384775 MS.gene56053:CDS 30.0%
! TTTTGCATCATCATTTGAGT+TGG + chr1.3:11384479-11384498 None:intergenic 30.0%
!! AAGAAGAAAAGTGCTACATT+TGG + chr1.3:11384406-11384425 None:intergenic 30.0%
ATGCAGGAAAAACAAAGTCA+AGG - chr1.3:11384275-11384294 MS.gene56053:CDS 35.0%
ATTAAAGGACAACCAGGAAA+AGG - chr1.3:11384325-11384344 MS.gene56053:CDS 35.0%
GATTTCACTACTTCGAAACT+TGG - chr1.3:11384688-11384707 MS.gene56053:CDS 35.0%
TAAAGTTTACTCTGTGCCTT+TGG - chr1.3:11384879-11384898 MS.gene56053:CDS 35.0%
TGCAGGAAAAACAAAGTCAA+GGG - chr1.3:11384276-11384295 MS.gene56053:CDS 35.0%
TGTGAAGAAAATGGCATCAA+AGG - chr1.3:11384625-11384644 MS.gene56053:CDS 35.0%
! GCAAAAGGTGTGAAGAAATT+GGG - chr1.3:11384778-11384797 MS.gene56053:CDS 35.0%
AAACAACATGATCCTGTGGA+AGG + chr1.3:11385032-11385051 None:intergenic 40.0%
AAAGCTTTGCGTGACATCAT+TGG - chr1.3:11384654-11384673 MS.gene56053:CDS 40.0%
AACATGTTCTCCATCAAGGA+AGG - chr1.3:11384562-11384581 MS.gene56053:CDS 40.0%
AGAAAATGGCATCAAAGGAG+AGG - chr1.3:11384630-11384649 MS.gene56053:CDS 40.0%
CCTTAACATGTTCTCCATCA+AGG - chr1.3:11384558-11384577 MS.gene56053:CDS 40.0%
CCTTGATGGAGAACATGTTA+AGG + chr1.3:11384561-11384580 None:intergenic 40.0%
CGTGACATCATTGGAATCTA+TGG - chr1.3:11384663-11384682 MS.gene56053:CDS 40.0%
GCAGGAAAAACAAAGTCAAG+GGG - chr1.3:11384277-11384296 MS.gene56053:CDS 40.0%
GTCACACTGATACTTCACAA+TGG - chr1.3:11384941-11384960 MS.gene56053:CDS 40.0%
TTCCACAGGATCATGTTGTT+TGG - chr1.3:11385031-11385050 MS.gene56053:CDS 40.0%
TTCTTCTGTTCCTTCCTTGA+TGG + chr1.3:11384575-11384594 None:intergenic 40.0%
! ATAGGAAAACCACCTTTTCC+TGG + chr1.3:11384340-11384359 None:intergenic 40.0%
! GGCAAAAGGTGTGAAGAAAT+TGG - chr1.3:11384777-11384796 MS.gene56053:CDS 40.0%
! TCCACAGGATCATGTTGTTT+GGG - chr1.3:11385032-11385051 MS.gene56053:CDS 40.0%
AAAGGACAACCAGGAAAAGG+TGG - chr1.3:11384328-11384347 MS.gene56053:CDS 45.0%
ACCCAAACAACATGATCCTG+TGG + chr1.3:11385036-11385055 None:intergenic 45.0%
CAAGTGCTCAAAGTTCAACC+TGG - chr1.3:11384985-11385004 MS.gene56053:CDS 45.0%
CTTCCATCAACACCTTCCAA+AGG + chr1.3:11384898-11384917 None:intergenic 45.0%
GTTTACTCTGTGCCTTTGGA+AGG - chr1.3:11384883-11384902 MS.gene56053:CDS 45.0%
TGACAAACTGGAACAGTTCC+AGG + chr1.3:11385006-11385025 None:intergenic 45.0%
TGTGGAAGGAAGTGACAAAC+TGG + chr1.3:11385018-11385037 None:intergenic 45.0%
! AGTTTGTCACTTCCTTCCAC+AGG - chr1.3:11385017-11385036 MS.gene56053:CDS 45.0%
!! GTGCCTTTGGAAGGTGTTGA+TGG - chr1.3:11384892-11384911 MS.gene56053:CDS 50.0%


Chromosome Type Strat End Strand Name
chr1.3 gene 11384265 11385065 11384265 ID=MS.gene56053
chr1.3 mRNA 11384265 11385065 11384265 ID=MS.gene56053.t1;Parent=MS.gene56053
chr1.3 exon 11384265 11385065 11384265 ID=MS.gene56053.t1.exon1;Parent=MS.gene56053.t1
chr1.3 CDS 11384265 11385065 11384265 ID=cds.MS.gene56053.t1;Parent=MS.gene56053.t1
Gene Sequence

>MS.gene56053

ATGAATGTTGATGCAGGAAAAACAAAGTCAAGGGGAACATCAGTCAACATAGAAAAAGATATTAAAGGACAACCAGGAAAAGGTGGTTTTCCTATGTTTTACAATTATGCTGCAAGTGAGACACAATTACATGATAAACCAAATGTAGCACTTTTCTTCTTAGAAAAGGATTTACATCAAGGAACAAAGTTGAACTTGCAATTCACAAAAACCAACTCAAATGATGATGCAAAATTCTTACCTAAAGAAGTTTCTAATTCAATACCTTTTTCTTCAAGCAAAGTACAAAACATCCTTAACATGTTCTCCATCAAGGAAGGAACAGAAGAATCTGAAATTGTGAAGAACACAATAAGTGAGTGTGAAGAAAATGGCATCAAAGGAGAGGAAAAGCTTTGCGTGACATCATTGGAATCTATGGTTGATTTCACTACTTCGAAACTTGGAAACAATGTTGAAGCCGTTTCAACAGAAGTGAAAAAAGAAAGTAGTGACTTGCAAGAATATGTAATGGCAAAAGGTGTGAAGAAATTGGGAGAGAAGAATAAAGCTGTAGTGTGTCACAAAGAGAATTACCCTTATGCAATTTTTTACTGTCATAAAACAGATACAACTAAAGTTTACTCTGTGCCTTTGGAAGGTGTTGATGGAAGTAGAGTTAAAGCTGTTGCTGTGTGTCACACTGATACTTCACAATGGAATCCTAAACATTTGGCTTTTCAAGTGCTCAAAGTTCAACCTGGAACTGTTCCAGTTTGTCACTTCCTTCCACAGGATCATGTTGTTTGGGTTTCCAAGTAA

Protein sequence

>MS.gene56053.t1

MNVDAGKTKSRGTSVNIEKDIKGQPGKGGFPMFYNYAASETQLHDKPNVALFFLEKDLHQGTKLNLQFTKTNSNDDAKFLPKEVSNSIPFSSSKVQNILNMFSIKEGTEESEIVKNTISECEENGIKGEEKLCVTSLESMVDFTTSKLGNNVEAVSTEVKKESSDLQEYVMAKGVKKLGEKNKAVVCHKENYPYAIFYCHKTDTTKVYSVPLEGVDGSRVKAVAVCHTDTSQWNPKHLAFQVLKVQPGTVPVCHFLPQDHVVWVSK