Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene99735.t1 | XP_003622266.1 | 91.2 | 194 | 10 | 2 | 44 | 231 | 156 | 348 | 2.50E-91 | 345.1 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene99735.t1 | Q9LU68 | 42.1 | 121 | 35 | 3 | 111 | 231 | 337 | 422 | 2.0e-12 | 74.3 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene99735.t1 | G7L5C3 | 91.2 | 194 | 10 | 2 | 44 | 231 | 156 | 348 | 1.8e-91 | 345.1 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|---|---|
| MS.gene99735.t1 | TR | Others |
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049150 | MS.gene99735 | 0.838942 | 2.13E-57 | -1.69E-46 |
| MS.gene049151 | MS.gene99735 | 0.816903 | 4.35E-52 | -1.69E-46 |
| MS.gene049153 | MS.gene99735 | 0.822967 | 1.78E-53 | -1.69E-46 |
| MS.gene049233 | MS.gene99735 | 0.816547 | 5.23E-52 | -1.69E-46 |
| MS.gene049260 | MS.gene99735 | 0.879277 | 1.42E-69 | -1.69E-46 |
| MS.gene049346 | MS.gene99735 | 0.834428 | 3.01E-56 | -1.69E-46 |
| MS.gene049599 | MS.gene99735 | -0.800029 | 1.75E-48 | -1.69E-46 |
| MS.gene049749 | MS.gene99735 | 0.80245 | 5.58E-49 | -1.69E-46 |
| MS.gene049758 | MS.gene99735 | 0.863987 | 1.68E-64 | -1.69E-46 |
| MS.gene049910 | MS.gene99735 | 0.80907 | 2.27E-50 | -1.69E-46 |
| MS.gene050020 | MS.gene99735 | 0.814463 | 1.52E-51 | -1.69E-46 |
| MS.gene050128 | MS.gene99735 | 0.858023 | 1.09E-62 | -1.69E-46 |
| MS.gene050301 | MS.gene99735 | 0.848681 | 5.25E-60 | -1.69E-46 |
| MS.gene050330 | MS.gene99735 | -0.810544 | 1.09E-50 | -1.69E-46 |
| MS.gene050438 | MS.gene99735 | 0.817521 | 3.16E-52 | -1.69E-46 |
| MS.gene050558 | MS.gene99735 | 0.839486 | 1.54E-57 | -1.69E-46 |
| MS.gene050586 | MS.gene99735 | 0.812258 | 4.64E-51 | -1.69E-46 |
| MS.gene050762 | MS.gene99735 | 0.806559 | 7.76E-50 | -1.69E-46 |
| MS.gene050763 | MS.gene99735 | 0.80586 | 1.09E-49 | -1.69E-46 |
| MS.gene050787 | MS.gene99735 | 0.811016 | 8.64E-51 | -1.69E-46 |
| MS.gene050807 | MS.gene99735 | 0.810494 | 1.12E-50 | -1.69E-46 |
| MS.gene05101 | MS.gene99735 | 0.813183 | 2.91E-51 | -1.69E-46 |
| MS.gene05119 | MS.gene99735 | -0.816566 | 5.18E-52 | -1.69E-46 |
| MS.gene051626 | MS.gene99735 | 0.821051 | 4.96E-53 | -1.69E-46 |
| MS.gene051663 | MS.gene99735 | 0.827872 | 1.23E-54 | -1.69E-46 |
| MS.gene051789 | MS.gene99735 | 0.850019 | 2.23E-60 | -1.69E-46 |
| MS.gene051860 | MS.gene99735 | 0.864336 | 1.31E-64 | -1.69E-46 |
| MS.gene052178 | MS.gene99735 | 0.868541 | 6.05E-66 | -1.69E-46 |
| MS.gene052207 | MS.gene99735 | 0.844366 | 7.91E-59 | -1.69E-46 |
| MS.gene052287 | MS.gene99735 | 0.81901 | 1.45E-52 | -1.69E-46 |
| MS.gene052340 | MS.gene99735 | 0.804889 | 1.74E-49 | -1.69E-46 |
| MS.gene052917 | MS.gene99735 | -0.82964 | 4.60E-55 | -1.69E-46 |
| MS.gene053074 | MS.gene99735 | 0.856109 | 4.02E-62 | -1.69E-46 |
| MS.gene053184 | MS.gene99735 | 0.815865 | 7.43E-52 | -1.69E-46 |
| MS.gene053204 | MS.gene99735 | 0.83188 | 1.30E-55 | -1.69E-46 |
| MS.gene053555 | MS.gene99735 | 0.831121 | 2.00E-55 | -1.69E-46 |
| MS.gene053557 | MS.gene99735 | 0.809926 | 1.49E-50 | -1.69E-46 |
| MS.gene053630 | MS.gene99735 | 0.818029 | 2.43E-52 | -1.69E-46 |
| MS.gene053836 | MS.gene99735 | 0.88313 | 5.82E-71 | -1.69E-46 |
| MS.gene054010 | MS.gene99735 | 0.840608 | 7.85E-58 | -1.69E-46 |
| MS.gene054011 | MS.gene99735 | 0.855339 | 6.74E-62 | -1.69E-46 |
| MS.gene054012 | MS.gene99735 | 0.841651 | 4.18E-58 | -1.69E-46 |
| MS.gene054013 | MS.gene99735 | 0.838914 | 2.17E-57 | -1.69E-46 |
| MS.gene054184 | MS.gene99735 | 0.836047 | 1.18E-56 | -1.69E-46 |
| MS.gene054185 | MS.gene99735 | 0.841866 | 3.66E-58 | -1.69E-46 |
| MS.gene054214 | MS.gene99735 | 0.847871 | 8.79E-60 | -1.69E-46 |
| MS.gene054379 | MS.gene99735 | 0.812503 | 4.10E-51 | -1.69E-46 |
| MS.gene054433 | MS.gene99735 | 0.803443 | 3.48E-49 | -1.69E-46 |
| MS.gene054473 | MS.gene99735 | 0.852999 | 3.19E-61 | -1.69E-46 |
| MS.gene054542 | MS.gene99735 | 0.839089 | 1.95E-57 | -1.69E-46 |
| MS.gene05456 | MS.gene99735 | 0.803513 | 3.36E-49 | -1.69E-46 |
| MS.gene054596 | MS.gene99735 | 0.830536 | 2.78E-55 | -1.69E-46 |
| MS.gene054618 | MS.gene99735 | 0.825598 | 4.30E-54 | -1.69E-46 |
| MS.gene054942 | MS.gene99735 | 0.804042 | 2.61E-49 | -1.69E-46 |
| MS.gene055207 | MS.gene99735 | 0.813151 | 2.96E-51 | -1.69E-46 |
| MS.gene055277 | MS.gene99735 | 0.844214 | 8.68E-59 | -1.69E-46 |
| MS.gene055303 | MS.gene99735 | 0.846345 | 2.30E-59 | -1.69E-46 |
| MS.gene055356 | MS.gene99735 | 0.814987 | 1.16E-51 | -1.69E-46 |
| MS.gene055358 | MS.gene99735 | 0.843636 | 1.24E-58 | -1.69E-46 |
| MS.gene055390 | MS.gene99735 | 0.826155 | 3.17E-54 | -1.69E-46 |
| MS.gene055422 | MS.gene99735 | 0.84444 | 7.55E-59 | -1.69E-46 |
| MS.gene055428 | MS.gene99735 | 0.843288 | 1.54E-58 | -1.69E-46 |
| MS.gene055604 | MS.gene99735 | 0.86605 | 3.78E-65 | -1.69E-46 |
| MS.gene055712 | MS.gene99735 | 0.82196 | 3.06E-53 | -1.69E-46 |
| MS.gene056069 | MS.gene99735 | -0.840624 | 7.77E-58 | -1.69E-46 |
| MS.gene056191 | MS.gene99735 | 0.80798 | 3.88E-50 | -1.69E-46 |
| MS.gene056612 | MS.gene99735 | 0.819656 | 1.04E-52 | -1.69E-46 |
| MS.gene056629 | MS.gene99735 | 0.811189 | 7.93E-51 | -1.69E-46 |
| MS.gene056772 | MS.gene99735 | 0.831562 | 1.56E-55 | -1.69E-46 |
| MS.gene056875 | MS.gene99735 | 0.808537 | 2.95E-50 | -1.69E-46 |
| MS.gene056902 | MS.gene99735 | 0.839918 | 1.19E-57 | -1.69E-46 |
| MS.gene056931 | MS.gene99735 | 0.804509 | 2.09E-49 | -1.69E-46 |
| MS.gene056933 | MS.gene99735 | 0.8033 | 3.72E-49 | -1.69E-46 |
| MS.gene05702 | MS.gene99735 | 0.809539 | 1.80E-50 | -1.69E-46 |
| MS.gene057121 | MS.gene99735 | 0.828029 | 1.13E-54 | -1.69E-46 |
| MS.gene057245 | MS.gene99735 | 0.805705 | 1.17E-49 | -1.69E-46 |
| MS.gene057260 | MS.gene99735 | 0.81714 | 3.85E-52 | -1.69E-46 |
| MS.gene057395 | MS.gene99735 | 0.815773 | 7.78E-52 | -1.69E-46 |
| MS.gene057439 | MS.gene99735 | 0.822968 | 1.78E-53 | -1.69E-46 |
| MS.gene057448 | MS.gene99735 | 0.803796 | 2.94E-49 | -1.69E-46 |
| MS.gene057450 | MS.gene99735 | 0.807251 | 5.54E-50 | -1.69E-46 |
| MS.gene057469 | MS.gene99735 | 0.844095 | 9.35E-59 | -1.69E-46 |
| MS.gene057752 | MS.gene99735 | 0.819402 | 1.18E-52 | -1.69E-46 |
| MS.gene057754 | MS.gene99735 | 0.801237 | 9.90E-49 | -1.69E-46 |
| MS.gene058002 | MS.gene99735 | 0.841315 | 5.12E-58 | -1.69E-46 |
| MS.gene05811 | MS.gene99735 | 0.853684 | 2.03E-61 | -1.69E-46 |
| MS.gene058142 | MS.gene99735 | 0.814904 | 1.21E-51 | -1.69E-46 |
| MS.gene058168 | MS.gene99735 | 0.817561 | 3.09E-52 | -1.69E-46 |
| MS.gene058236 | MS.gene99735 | 0.821467 | 3.98E-53 | -1.69E-46 |
| MS.gene058484 | MS.gene99735 | 0.807887 | 4.06E-50 | -1.69E-46 |
| MS.gene058749 | MS.gene99735 | 0.814469 | 1.52E-51 | -1.69E-46 |
| MS.gene059006 | MS.gene99735 | 0.835519 | 1.60E-56 | -1.69E-46 |
| MS.gene05907 | MS.gene99735 | 0.823167 | 1.60E-53 | -1.69E-46 |
| MS.gene059124 | MS.gene99735 | 0.810378 | 1.19E-50 | -1.69E-46 |
| MS.gene059128 | MS.gene99735 | 0.809085 | 2.25E-50 | -1.69E-46 |
| MS.gene059228 | MS.gene99735 | 0.819242 | 1.29E-52 | -1.69E-46 |
| MS.gene059294 | MS.gene99735 | 0.813174 | 2.92E-51 | -1.69E-46 |
| MS.gene059518 | MS.gene99735 | 0.835636 | 1.50E-56 | -1.69E-46 |
| MS.gene05984 | MS.gene99735 | 0.836382 | 9.67E-57 | -1.69E-46 |
| MS.gene059894 | MS.gene99735 | 0.818275 | 2.13E-52 | -1.69E-46 |
| MS.gene059974 | MS.gene99735 | 0.806097 | 9.70E-50 | -1.69E-46 |
| MS.gene060005 | MS.gene99735 | 0.808374 | 3.20E-50 | -1.69E-46 |
| MS.gene060314 | MS.gene99735 | 0.80011 | 1.68E-48 | -1.69E-46 |
| MS.gene06037 | MS.gene99735 | 0.861959 | 7.11E-64 | -1.69E-46 |
| MS.gene060476 | MS.gene99735 | 0.841059 | 5.98E-58 | -1.69E-46 |
| MS.gene060495 | MS.gene99735 | 0.806444 | 8.20E-50 | -1.69E-46 |
| MS.gene060559 | MS.gene99735 | 0.816868 | 4.43E-52 | -1.69E-46 |
| MS.gene061049 | MS.gene99735 | 0.859492 | 3.98E-63 | -1.69E-46 |
| MS.gene061112 | MS.gene99735 | 0.821788 | 3.35E-53 | -1.69E-46 |
| MS.gene061113 | MS.gene99735 | 0.808016 | 3.81E-50 | -1.69E-46 |
| MS.gene061265 | MS.gene99735 | 0.820313 | 7.33E-53 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene99735.t1 | MTR_7g032240 | 89.451 | 237 | 14 | 3 | 1 | 231 | 117 | 348 | 1.58e-141 | 400 |
| MS.gene99735.t1 | MTR_5g072780 | 38.710 | 124 | 43 | 3 | 108 | 231 | 123 | 213 | 3.55e-17 | 77.8 |
| MS.gene99735.t1 | MTR_4g127420 | 38.793 | 116 | 42 | 3 | 114 | 229 | 293 | 379 | 6.58e-16 | 76.3 |
| MS.gene99735.t1 | MTR_1g110870 | 38.889 | 126 | 54 | 4 | 109 | 231 | 316 | 421 | 3.71e-15 | 74.3 |
| MS.gene99735.t1 | MTR_8g104190 | 35.366 | 164 | 56 | 7 | 76 | 231 | 276 | 397 | 4.20e-15 | 73.9 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene99735.t1 | AT5G14370 | 51.934 | 181 | 58 | 10 | 56 | 231 | 177 | 333 | 5.04e-38 | 135 |
| MS.gene99735.t1 | AT5G57180 | 42.857 | 119 | 33 | 3 | 111 | 229 | 337 | 420 | 1.84e-19 | 86.7 |
| MS.gene99735.t1 | AT5G57180 | 42.149 | 121 | 35 | 3 | 111 | 231 | 337 | 422 | 5.45e-19 | 85.5 |
| MS.gene99735.t1 | AT4G25990 | 41.176 | 119 | 37 | 2 | 111 | 229 | 293 | 378 | 7.93e-18 | 81.6 |
| MS.gene99735.t1 | AT1G73870 | 41.270 | 126 | 55 | 5 | 108 | 231 | 276 | 384 | 1.07e-17 | 81.3 |
| MS.gene99735.t1 | AT4G25990 | 41.176 | 119 | 37 | 2 | 111 | 229 | 293 | 378 | 1.58e-17 | 80.9 |
| MS.gene99735.t1 | AT4G25990 | 40.496 | 121 | 39 | 2 | 111 | 231 | 293 | 380 | 2.82e-17 | 80.1 |
| MS.gene99735.t1 | AT1G25440 | 38.710 | 124 | 59 | 4 | 109 | 231 | 293 | 400 | 1.56e-15 | 75.5 |
| MS.gene99735.t1 | AT1G07050 | 82.051 | 39 | 7 | 0 | 191 | 229 | 150 | 188 | 5.88e-15 | 71.2 |
| MS.gene99735.t1 | AT1G07050 | 78.049 | 41 | 9 | 0 | 191 | 231 | 150 | 190 | 2.02e-14 | 69.7 |
| MS.gene99735.t1 | AT1G68520 | 35.484 | 124 | 65 | 4 | 108 | 231 | 288 | 396 | 3.11e-13 | 68.6 |
Find 61 sgRNAs with CRISPR-Local
Find 121 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TGGTATTTGTACAGGTATTA+TGG | 0.141690 | 7.4:+69817923 | MS.gene99735:intron |
| GGTTATGGGTGTGATGTTAA+TGG | 0.287554 | 7.4:+69817949 | MS.gene99735:CDS |
| GCAACTTGCAACATTAGTTT+TGG | 0.300735 | 7.4:-69818176 | None:intergenic |
| TTGCTAGCTCCAACACCTTC+AGG | 0.321383 | 7.4:-69817988 | None:intergenic |
| GGTATTTGTACAGGTATTAT+GGG | 0.322694 | 7.4:+69817924 | MS.gene99735:intron |
| TTGAATGCTTGGTCTGATAA+AGG | 0.338505 | 7.4:+69816680 | MS.gene99735:CDS |
| CAGGTATTATGGGATGGTTA+TGG | 0.341791 | 7.4:+69817934 | MS.gene99735:intron |
| AGGTATTATGGGATGGTTAT+GGG | 0.355741 | 7.4:+69817935 | MS.gene99735:intron |
| TGAAGAGAGTGAAGAGAGAT+GGG | 0.373318 | 7.4:+69816529 | MS.gene99735:CDS |
| AAAGGTTCTCTTTATGTTGA+TGG | 0.373469 | 7.4:+69816698 | MS.gene99735:CDS |
| CAGACTGTTCCTGATTTGTT+CGG | 0.385801 | 7.4:+69816737 | MS.gene99735:CDS |
| TGTGAAGGTGAAGGAAGAAA+TGG | 0.408295 | 7.4:+69818017 | MS.gene99735:CDS |
| ATGAAGAGAGTGAAGAGAGA+TGG | 0.416748 | 7.4:+69816528 | MS.gene99735:CDS |
| GAAGAAATGGGTTGGAAGCT+TGG | 0.419659 | 7.4:+69818030 | MS.gene99735:CDS |
| AAGAAATGGGTTGGAAGCTT+GGG | 0.434227 | 7.4:+69818031 | MS.gene99735:CDS |
| TGGAATAGTACCTTCATCCT+AGG | 0.443663 | 7.4:-69818156 | None:intergenic |
| ATTGAACCACCGAACAAATC+AGG | 0.449972 | 7.4:-69816746 | None:intergenic |
| GGAAAGCAATGAGGAGGAAG+AGG | 0.454954 | 7.4:+69816498 | MS.gene99735:CDS |
| GTTATGGGTGTGATGTTAAT+GGG | 0.457104 | 7.4:+69817950 | MS.gene99735:CDS |
| GACGGTGGAGCAACGCTAAA+CGG | 0.458187 | 7.4:+69816365 | MS.gene99735:CDS |
| GAAGTTAATAGTACTTGTGT+TGG | 0.464035 | 7.4:+69816599 | MS.gene99735:CDS |
| AGGTGTCCGGTGATGGATCA+AGG | 0.465230 | 7.4:+69816467 | MS.gene99735:CDS |
| AGAGACGAAGAAGGAAATGA+TGG | 0.504022 | 7.4:+69816574 | MS.gene99735:CDS |
| ATTAGTAAAATTACATTGGG+AGG | 0.505856 | 7.4:-69816770 | None:intergenic |
| AGTGATGAGGTGTCCGGTGA+TGG | 0.506901 | 7.4:+69816460 | MS.gene99735:CDS |
| AGTTGTTGAAGAGACGAAGA+AGG | 0.513266 | 7.4:+69816565 | MS.gene99735:CDS |
| TTAGTAAAATTACATTGGGA+GGG | 0.521925 | 7.4:-69816769 | None:intergenic |
| GACGACGACGGAGGAAGAAG+AGG | 0.527666 | 7.4:+69816427 | MS.gene99735:CDS |
| AAAGCAGACTCTTTGCTAAG+AGG | 0.532652 | 7.4:+69818094 | MS.gene99735:CDS |
| ATGATGGGATATTGAATGCT+TGG | 0.532795 | 7.4:+69816669 | MS.gene99735:CDS |
| TGTTGAGATACAAAGAGAAG+AGG | 0.545019 | 7.4:+69818070 | MS.gene99735:CDS |
| TTTGTACAGGTATTATGGGA+TGG | 0.546223 | 7.4:+69817928 | MS.gene99735:intron |
| TTATGTTGATGGTTGTGATG+AGG | 0.548680 | 7.4:+69816709 | MS.gene99735:CDS |
| TCCGGTGATGGATCAAGGGT+TGG | 0.551320 | 7.4:+69816472 | MS.gene99735:CDS |
| ACTGTTCCTGATTTGTTCGG+TGG | 0.551576 | 7.4:+69816740 | MS.gene99735:CDS |
| TGGAAAGTTCCTGAAGGTGT+TGG | 0.558300 | 7.4:+69817979 | MS.gene99735:CDS |
| GTTTGAAGCTTGATCATGAT+GGG | 0.563359 | 7.4:+69816654 | MS.gene99735:CDS |
| AGTTTGAAGCTTGATCATGA+TGG | 0.572212 | 7.4:+69816653 | MS.gene99735:CDS |
| TCAACTTGCGAACTTCATAA+CGG | 0.572673 | 7.4:-69818119 | None:intergenic |
| GACGAAGAAGGAAATGATGG+AGG | 0.575278 | 7.4:+69816577 | MS.gene99735:CDS |
| GTGAAGGTGAAGGAAGAAAT+GGG | 0.575413 | 7.4:+69818018 | MS.gene99735:CDS |
| ACCAACCCTTGATCCATCAC+CGG | 0.584061 | 7.4:-69816473 | None:intergenic |
| CAATAACAATGTGAAGGTGA+AGG | 0.584908 | 7.4:+69818008 | MS.gene99735:CDS |
| TGGTGAGGAAAGCAATGAGG+AGG | 0.586113 | 7.4:+69816492 | MS.gene99735:CDS |
| AGGTGAAGGAAGAAATGGGT+TGG | 0.586587 | 7.4:+69818022 | MS.gene99735:CDS |
| GTTTAGCGTTGCTCCACCGT+CGG | 0.602611 | 7.4:-69816363 | None:intergenic |
| ATTGTCAGTGATGAGGTGTC+CGG | 0.606087 | 7.4:+69816454 | MS.gene99735:CDS |
| TGATGGATCAAGGGTTGGTG+AGG | 0.611319 | 7.4:+69816477 | MS.gene99735:CDS |
| TTGGAAGCTTGGGCAGAGAG+AGG | 0.619401 | 7.4:+69818041 | MS.gene99735:CDS |
| AACAAATCAGGAACAGTCTG+AGG | 0.628449 | 7.4:-69816734 | None:intergenic |
| AACGGTGATGACGACGACGG+AGG | 0.631073 | 7.4:+69816418 | MS.gene99735:CDS |
| AGCTAGCAATAACAATGTGA+AGG | 0.640407 | 7.4:+69818002 | MS.gene99735:CDS |
| AGGAGTCATTGTCAGTGATG+AGG | 0.647629 | 7.4:+69816447 | MS.gene99735:CDS |
| AACACATGGAAAGTTCCTGA+AGG | 0.666366 | 7.4:+69817973 | MS.gene99735:CDS |
| GTGATGTTAATGGGAACACA+TGG | 0.674682 | 7.4:+69817959 | MS.gene99735:CDS |
| GGTTGGTGAGGAAAGCAATG+AGG | 0.677201 | 7.4:+69816489 | MS.gene99735:CDS |
| GCGTTACTCTTCACATCCGA+CGG | 0.677661 | 7.4:+69816347 | MS.gene99735:CDS |
| GGTGTCCGGTGATGGATCAA+GGG | 0.677873 | 7.4:+69816468 | MS.gene99735:CDS |
| TTACTCTTCACATCCGACGG+TGG | 0.716926 | 7.4:+69816350 | MS.gene99735:CDS |
| TGAATCGACTACGTTAACAA+CGG | 0.720555 | 7.4:+69816400 | MS.gene99735:CDS |
| AACAACGGTGATGACGACGA+CGG | 0.737423 | 7.4:+69816415 | MS.gene99735:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AAAAATTAGTAAAATTACAT+TGG | - | chr7.4:69816777-69816796 | None:intergenic | 10.0% |
| !! | AAAATTAGTAAAATTACATT+GGG | - | chr7.4:69816776-69816795 | None:intergenic | 10.0% |
| !! | ATCAATTTAGAAATATATTA+TGG | + | chr7.4:69817857-69817876 | MS.gene99735:intron | 10.0% |
| !! | AAAATCAATTTAAACCATTA+TGG | - | chr7.4:69817706-69817725 | None:intergenic | 15.0% |
| !!! | ATGGTTTAAATTGATTTTAT+AGG | + | chr7.4:69817708-69817727 | MS.gene99735:intron | 15.0% |
| !!! | TTTTATTATTGGTTACTTTT+TGG | + | chr7.4:69817431-69817450 | MS.gene99735:intron | 15.0% |
| !! | AACCAATAATAAAAAGTTGT+CGG | - | chr7.4:69817425-69817444 | None:intergenic | 20.0% |
| !! | ACTTGATGCAAATTATAATT+TGG | - | chr7.4:69817114-69817133 | None:intergenic | 20.0% |
| !! | TATTATATATCACAACCAAA+CGG | - | chr7.4:69817549-69817568 | None:intergenic | 20.0% |
| !!! | ATTTTTTCCAATTAATTGAG+TGG | + | chr7.4:69816987-69817006 | MS.gene99735:intron | 20.0% |
| !!! | TCAATGATTTGTTTTTAAAC+TGG | - | chr7.4:69817223-69817242 | None:intergenic | 20.0% |
| ! | ATAATAATTGTGACTCTTGT+TGG | + | chr7.4:69817563-69817582 | MS.gene99735:intron | 25.0% |
| ! | ATGTGAAGAAATAGGAAATT+TGG | + | chr7.4:69817024-69817043 | MS.gene99735:intron | 25.0% |
| ! | ATTAGTAAAATTACATTGGG+AGG | - | chr7.4:69816773-69816792 | None:intergenic | 25.0% |
| ! | ATTATATATCACAACCAAAC+GGG | - | chr7.4:69817548-69817567 | None:intergenic | 25.0% |
| ! | CACTTTAAATGTGAAGAAAT+AGG | + | chr7.4:69817016-69817035 | MS.gene99735:intron | 25.0% |
| ! | TTAGTAAAATTACATTGGGA+GGG | - | chr7.4:69816772-69816791 | None:intergenic | 25.0% |
| ! | TTCAATTTATTGCATCAACT+TGG | - | chr7.4:69817799-69817818 | None:intergenic | 25.0% |
| !! | ATCTTACATTTAAAACACGA+TGG | + | chr7.4:69817475-69817494 | MS.gene99735:intron | 25.0% |
| !! | ATTTCTTTTGTGCATGTTTT+TGG | + | chr7.4:69817369-69817388 | MS.gene99735:intron | 25.0% |
| !! | GAATAGTGATGATTTCATTT+TGG | + | chr7.4:69817397-69817416 | MS.gene99735:intron | 25.0% |
| !! | GATACAAGAGAAAATCTTTT+AGG | + | chr7.4:69817825-69817844 | MS.gene99735:intron | 25.0% |
| !! | GATTTTATAGGATTGAGTTA+TGG | + | chr7.4:69817720-69817739 | MS.gene99735:intron | 25.0% |
| !! | TTCATTTGATAGTAACAAGT+GGG | - | chr7.4:69817884-69817903 | None:intergenic | 25.0% |
| !! | TTTTTAATCAGTTATGAGTG+TGG | + | chr7.4:69817272-69817291 | MS.gene99735:intron | 25.0% |
| AAAGGTTCTCTTTATGTTGA+TGG | + | chr7.4:69816698-69816717 | MS.gene99735:CDS | 30.0% | |
| AAGCTATTTGGTATTTGTAC+AGG | + | chr7.4:69817915-69817934 | MS.gene99735:intron | 30.0% | |
| GAAGTTAATAGTACTTGTGT+TGG | + | chr7.4:69816599-69816618 | MS.gene99735:CDS | 30.0% | |
| GGCTTAAATCATGAACTAAA+TGG | - | chr7.4:69817093-69817112 | None:intergenic | 30.0% | |
| GGTATTTGTACAGGTATTAT+GGG | + | chr7.4:69817924-69817943 | MS.gene99735:intron | 30.0% | |
| TGGTATTTGTACAGGTATTA+TGG | + | chr7.4:69817923-69817942 | MS.gene99735:intron | 30.0% | |
| TTATATATCACAACCAAACG+GGG | - | chr7.4:69817547-69817566 | None:intergenic | 30.0% | |
| TTTATGTGTGACATCCATAA+TGG | + | chr7.4:69817689-69817708 | MS.gene99735:intron | 30.0% | |
| ! | ACAAGAGAAAATCTTTTAGG+TGG | + | chr7.4:69817828-69817847 | MS.gene99735:intron | 30.0% |
| ! | CACCGACAACTTTTTATTAT+TGG | + | chr7.4:69817420-69817439 | MS.gene99735:intron | 30.0% |
| ! | CTTCATTTGATAGTAACAAG+TGG | - | chr7.4:69817885-69817904 | None:intergenic | 30.0% |
| ! | TTCTTTTACCCGTATTTCAA+TGG | + | chr7.4:69817521-69817540 | MS.gene99735:intron | 30.0% |
| !!! | AGTGTTTTGATGAAGCTATT+TGG | + | chr7.4:69817903-69817922 | MS.gene99735:intron | 30.0% |
| !!! | ATCTCTCAATTATTTTGACG+AGG | + | chr7.4:69817768-69817787 | MS.gene99735:intron | 30.0% |
| !!! | TATTGGTTACTTTTTGGCAA+GGG | + | chr7.4:69817437-69817456 | MS.gene99735:intron | 30.0% |
| !!! | TTATTGGTTACTTTTTGGCA+AGG | + | chr7.4:69817436-69817455 | MS.gene99735:intron | 30.0% |
| AGCTAGCAATAACAATGTGA+AGG | + | chr7.4:69818002-69818021 | MS.gene99735:CDS | 35.0% | |
| AGGTATTATGGGATGGTTAT+GGG | + | chr7.4:69817935-69817954 | MS.gene99735:intron | 35.0% | |
| ATAATTGTGACTCTTGTTGG+AGG | + | chr7.4:69817566-69817585 | MS.gene99735:intron | 35.0% | |
| ATGTAAGATCCTAATCAAGC+AGG | - | chr7.4:69817464-69817483 | None:intergenic | 35.0% | |
| CAATAACAATGTGAAGGTGA+AGG | + | chr7.4:69818008-69818027 | MS.gene99735:CDS | 35.0% | |
| GAACTAAATGGTAAGCTACA+AGG | - | chr7.4:69817081-69817100 | None:intergenic | 35.0% | |
| GCTTGAAGTTGGCAAAATTA+TGG | - | chr7.4:69816951-69816970 | None:intergenic | 35.0% | |
| GTGTAAGCCACTCAATTAAT+TGG | - | chr7.4:69816997-69817016 | None:intergenic | 35.0% | |
| GTTATGGGTGTGATGTTAAT+GGG | + | chr7.4:69817950-69817969 | MS.gene99735:CDS | 35.0% | |
| TCAACTTGCGAACTTCATAA+CGG | - | chr7.4:69818122-69818141 | None:intergenic | 35.0% | |
| TGAATCGACTACGTTAACAA+CGG | + | chr7.4:69816400-69816419 | MS.gene99735:CDS | 35.0% | |
| TGTTGAGATACAAAGAGAAG+AGG | + | chr7.4:69818070-69818089 | MS.gene99735:CDS | 35.0% | |
| TTGAATGCTTGGTCTGATAA+AGG | + | chr7.4:69816680-69816699 | MS.gene99735:CDS | 35.0% | |
| TTTGTACAGGTATTATGGGA+TGG | + | chr7.4:69817928-69817947 | MS.gene99735:intron | 35.0% | |
| ! | AGTGATTTTGCCAACTTCAA+AGG | - | chr7.4:69816922-69816941 | None:intergenic | 35.0% |
| ! | AGTTTGAAGCTTGATCATGA+TGG | + | chr7.4:69816653-69816672 | MS.gene99735:CDS | 35.0% |
| ! | ATGATGGGATATTGAATGCT+TGG | + | chr7.4:69816669-69816688 | MS.gene99735:CDS | 35.0% |
| ! | GTTTGAAGCTTGATCATGAT+GGG | + | chr7.4:69816654-69816673 | MS.gene99735:CDS | 35.0% |
| !! | TTATGTTGATGGTTGTGATG+AGG | + | chr7.4:69816709-69816728 | MS.gene99735:CDS | 35.0% |
| !! | TTGTGCATGTGACTGATTTT+TGG | + | chr7.4:69817334-69817353 | MS.gene99735:intron | 35.0% |
| !!! | ATTGGTTACTTTTTGGCAAG+GGG | + | chr7.4:69817438-69817457 | MS.gene99735:intron | 35.0% |
| !!! | CTTTTGTGCATGTTTTTGGA+TGG | + | chr7.4:69817373-69817392 | MS.gene99735:intron | 35.0% |
| !!! | TTGAAACGACGTCGTTTTTT+GGG | + | chr7.4:69816624-69816643 | MS.gene99735:CDS | 35.0% |
| !!! | TTTGAAACGACGTCGTTTTT+TGG | + | chr7.4:69816623-69816642 | MS.gene99735:CDS | 35.0% |
| AAAGCAGACTCTTTGCTAAG+AGG | + | chr7.4:69818094-69818113 | MS.gene99735:CDS | 40.0% | |
| AACAAATCAGGAACAGTCTG+AGG | - | chr7.4:69816737-69816756 | None:intergenic | 40.0% | |
| AACACATGGAAAGTTCCTGA+AGG | + | chr7.4:69817973-69817992 | MS.gene99735:CDS | 40.0% | |
| AGAGACGAAGAAGGAAATGA+TGG | + | chr7.4:69816574-69816593 | MS.gene99735:CDS | 40.0% | |
| AGTTGTTGAAGAGACGAAGA+AGG | + | chr7.4:69816565-69816584 | MS.gene99735:CDS | 40.0% | |
| ATGAAGAGAGTGAAGAGAGA+TGG | + | chr7.4:69816528-69816547 | MS.gene99735:CDS | 40.0% | |
| ATTGAACCACCGAACAAATC+AGG | - | chr7.4:69816749-69816768 | None:intergenic | 40.0% | |
| CAGGTATTATGGGATGGTTA+TGG | + | chr7.4:69817934-69817953 | MS.gene99735:intron | 40.0% | |
| GATTGAGTTATGGTGTGACA+AGG | + | chr7.4:69817730-69817749 | MS.gene99735:intron | 40.0% | |
| GGTTATGGGTGTGATGTTAA+TGG | + | chr7.4:69817949-69817968 | MS.gene99735:CDS | 40.0% | |
| GTGAAGGTGAAGGAAGAAAT+GGG | + | chr7.4:69818018-69818037 | MS.gene99735:CDS | 40.0% | |
| GTGATGTTAATGGGAACACA+TGG | + | chr7.4:69817959-69817978 | MS.gene99735:CDS | 40.0% | |
| TGAAGAGAGTGAAGAGAGAT+GGG | + | chr7.4:69816529-69816548 | MS.gene99735:CDS | 40.0% | |
| TGGAATAGTACCTTCATCCT+AGG | - | chr7.4:69818159-69818178 | None:intergenic | 40.0% | |
| TGTGAAGGTGAAGGAAGAAA+TGG | + | chr7.4:69818017-69818036 | MS.gene99735:CDS | 40.0% | |
| ! | ATTTTGCCAACTTCAAGCTG+AGG | + | chr7.4:69816953-69816972 | MS.gene99735:intron | 40.0% |
| ! | GAAATTTGGAGCTCGAATCT+CGG | + | chr7.4:69817038-69817057 | MS.gene99735:intron | 40.0% |
| ! | GCAAGTTGAACGCTGAAAAA+CGG | + | chr7.4:69818133-69818152 | MS.gene99735:CDS | 40.0% |
| !! | AAGAAATGGGTTGGAAGCTT+GGG | + | chr7.4:69818031-69818050 | MS.gene99735:CDS | 40.0% |
| !! | CAGACTGTTCCTGATTTGTT+CGG | + | chr7.4:69816737-69816756 | MS.gene99735:CDS | 40.0% |
| AAACGGGGCCATTGAAATAC+GGG | - | chr7.4:69817532-69817551 | None:intergenic | 45.0% | |
| AGGAGTCATTGTCAGTGATG+AGG | + | chr7.4:69816447-69816466 | MS.gene99735:CDS | 45.0% | |
| AGGTGAAGGAAGAAATGGGT+TGG | + | chr7.4:69818022-69818041 | MS.gene99735:CDS | 45.0% | |
| CAAACGGGGCCATTGAAATA+CGG | - | chr7.4:69817533-69817552 | None:intergenic | 45.0% | |
| GACGAAGAAGGAAATGATGG+AGG | + | chr7.4:69816577-69816596 | MS.gene99735:CDS | 45.0% | |
| TCATCTCCTCAGCTTGAAGT+TGG | - | chr7.4:69816962-69816981 | None:intergenic | 45.0% | |
| TGAAAAACGGCCTAGGATGA+AGG | + | chr7.4:69818146-69818165 | MS.gene99735:CDS | 45.0% | |
| TTGCCAACTTCAAAGCCAGT+CGG | - | chr7.4:69816877-69816896 | None:intergenic | 45.0% | |
| ! | ATTGTCAGTGATGAGGTGTC+CGG | + | chr7.4:69816454-69816473 | MS.gene99735:CDS | 45.0% |
| ! | TGGAAAGTTCCTGAAGGTGT+TGG | + | chr7.4:69817979-69817998 | MS.gene99735:CDS | 45.0% |
| !! | ACTGTTCCTGATTTGTTCGG+TGG | + | chr7.4:69816740-69816759 | MS.gene99735:CDS | 45.0% |
| !! | GAAGAAATGGGTTGGAAGCT+TGG | + | chr7.4:69818030-69818049 | MS.gene99735:CDS | 45.0% |
| !! | GCGATGATTGCCTTTGAAGT+TGG | + | chr7.4:69816909-69816928 | MS.gene99735:intron | 45.0% |
| ACCAACCCTTGATCCATCAC+CGG | - | chr7.4:69816476-69816495 | None:intergenic | 50.0% | |
| CAGTGTTATCGTGATCCGAC+TGG | + | chr7.4:69816859-69816878 | MS.gene99735:intron | 50.0% | |
| CGTATTTCAATGGCCCCGTT+TGG | + | chr7.4:69817531-69817550 | MS.gene99735:intron | 50.0% | |
| GCGTTACTCTTCACATCCGA+CGG | + | chr7.4:69816347-69816366 | MS.gene99735:CDS | 50.0% | |
| GGAAAGCAATGAGGAGGAAG+AGG | + | chr7.4:69816498-69816517 | MS.gene99735:CDS | 50.0% | |
| TGAACGCTGAAAAACGGCCT+AGG | + | chr7.4:69818139-69818158 | MS.gene99735:CDS | 50.0% | |
| TGGTGAGGAAAGCAATGAGG+AGG | + | chr7.4:69816492-69816511 | MS.gene99735:CDS | 50.0% | |
| TTACTCTTCACATCCGACGG+TGG | + | chr7.4:69816350-69816369 | MS.gene99735:CDS | 50.0% | |
| TTGCTAGCTCCAACACCTTC+AGG | - | chr7.4:69817991-69818010 | None:intergenic | 50.0% | |
| ! | AACAACGGTGATGACGACGA+CGG | + | chr7.4:69816415-69816434 | MS.gene99735:CDS | 50.0% |
| !! | GATCCGACTGGCTTTGAAGT+TGG | + | chr7.4:69816871-69816890 | MS.gene99735:intron | 50.0% |
| !! | GGTTGGTGAGGAAAGCAATG+AGG | + | chr7.4:69816489-69816508 | MS.gene99735:CDS | 50.0% |
| !! | TGATGGATCAAGGGTTGGTG+AGG | + | chr7.4:69816477-69816496 | MS.gene99735:CDS | 50.0% |
| GACGGTGGAGCAACGCTAAA+CGG | + | chr7.4:69816365-69816384 | MS.gene99735:CDS | 55.0% | |
| GTTTAGCGTTGCTCCACCGT+CGG | - | chr7.4:69816366-69816385 | None:intergenic | 55.0% | |
| TTGGAAGCTTGGGCAGAGAG+AGG | + | chr7.4:69818041-69818060 | MS.gene99735:CDS | 55.0% | |
| ! | AGTGATGAGGTGTCCGGTGA+TGG | + | chr7.4:69816460-69816479 | MS.gene99735:CDS | 55.0% |
| !! | AGGTGTCCGGTGATGGATCA+AGG | + | chr7.4:69816467-69816486 | MS.gene99735:CDS | 55.0% |
| !! | GGTGTCCGGTGATGGATCAA+GGG | + | chr7.4:69816468-69816487 | MS.gene99735:CDS | 55.0% |
| !! | TCCGGTGATGGATCAAGGGT+TGG | + | chr7.4:69816472-69816491 | MS.gene99735:CDS | 55.0% |
| GACGACGACGGAGGAAGAAG+AGG | + | chr7.4:69816427-69816446 | MS.gene99735:CDS | 60.0% | |
| GGCAAGGGGCCTGCTTGATT+AGG | + | chr7.4:69817452-69817471 | MS.gene99735:intron | 60.0% | |
| ! | AACGGTGATGACGACGACGG+AGG | + | chr7.4:69816418-69816437 | MS.gene99735:CDS | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr7.4 | gene | 69816326 | 69818185 | 69816326 | ID=MS.gene99735 |
| chr7.4 | mRNA | 69816326 | 69818185 | 69816326 | ID=MS.gene99735.t1;Parent=MS.gene99735 |
| chr7.4 | exon | 69816326 | 69816778 | 69816326 | ID=MS.gene99735.t1.exon1;Parent=MS.gene99735.t1 |
| chr7.4 | CDS | 69816326 | 69816778 | 69816326 | ID=cds.MS.gene99735.t1;Parent=MS.gene99735.t1 |
| chr7.4 | exon | 69817937 | 69818185 | 69817937 | ID=MS.gene99735.t1.exon2;Parent=MS.gene99735.t1 |
| chr7.4 | CDS | 69817937 | 69818185 | 69817937 | ID=cds.MS.gene99735.t1;Parent=MS.gene99735.t1 |
| Gene Sequence |
| Protein sequence |
