AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene066193

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene066193.t1 MTR_2g064310 97.765 358 8 0 1 358 1 358 0.0 712
MS.gene066193.t1 MTR_3g081640 61.732 358 121 6 11 358 10 361 2.71e-151 431
MS.gene066193.t1 MTR_3g081580 60.845 355 126 5 11 358 10 358 9.28e-146 416
MS.gene066193.t1 MTR_3g082050 59.538 346 131 4 15 358 11 349 5.27e-143 409
MS.gene066193.t1 MTR_3g082050 59.195 348 133 4 13 358 37 377 8.86e-141 404
MS.gene066193.t1 MTR_3g082050 62.813 320 113 3 41 358 57 372 2.99e-140 403
MS.gene066193.t1 MTR_3g081690 57.798 327 129 4 15 339 11 330 1.08e-127 370
MS.gene066193.t1 MTR_3g081690 61.130 301 111 3 41 339 57 353 5.17e-125 364
MS.gene066193.t1 MTR_4g083570 48.696 345 170 3 15 358 12 350 4.60e-116 340
MS.gene066193.t1 MTR_8g105030 48.169 355 155 7 11 352 9 347 1.44e-108 323
MS.gene066193.t1 MTR_6g007687 48.138 349 172 3 7 355 5 344 1.94e-106 316
MS.gene066193.t1 MTR_3g104400 44.904 363 164 4 30 358 5 365 5.28e-101 303
MS.gene066193.t1 MTR_1g016120 44.353 363 167 7 30 358 13 374 8.60e-95 287
MS.gene066193.t1 MTR_5g071990 39.583 336 177 6 28 358 4 318 2.26e-77 241
MS.gene066193.t1 MTR_3g081690 56.087 230 92 4 115 339 14 239 1.36e-76 236
MS.gene066193.t1 MTR_3g081690 66.460 161 47 1 196 356 52 205 4.57e-69 216
MS.gene066193.t1 MTR_3g081690 70.833 144 42 0 196 339 74 217 2.64e-68 214
MS.gene066193.t1 MTR_3g081630 56.164 73 30 2 84 155 23 94 9.35e-18 79.3
MS.gene066193.t1 MTR_4g006710 49.351 77 38 1 52 128 38 113 2.97e-16 74.3
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene066193.t1 AT3G12750 58.953 363 126 7 4 358 8 355 2.97e-146 417
MS.gene066193.t1 AT1G05300 54.971 342 142 4 24 358 24 360 8.37e-127 368
MS.gene066193.t1 AT2G32270 53.963 328 127 3 31 358 36 339 4.67e-119 348
MS.gene066193.t1 AT5G62160 49.861 361 171 5 2 358 1 355 1.83e-110 327
MS.gene066193.t1 AT1G31260 47.522 343 175 2 20 358 23 364 2.60e-110 327
MS.gene066193.t1 AT4G19690 44.915 354 181 2 5 358 8 347 3.25e-100 300
MS.gene066193.t1 AT2G04032 42.897 359 197 3 5 358 10 365 1.15e-97 294
MS.gene066193.t1 AT4G19680 46.341 328 170 4 31 358 29 350 1.36e-97 293
MS.gene066193.t1 AT1G10970 43.288 365 170 3 31 358 10 374 4.65e-95 288
MS.gene066193.t1 AT1G10970 43.288 365 170 3 31 358 10 374 4.65e-95 288
MS.gene066193.t1 AT1G60960 41.579 380 170 4 31 358 36 415 1.44e-90 278
MS.gene066193.t1 AT1G60960 41.579 380 170 4 31 358 46 425 1.77e-90 278
MS.gene066193.t1 AT4G33020 38.734 395 193 9 9 358 1 391 6.72e-85 262
MS.gene066193.t1 AT4G33020 41.379 348 158 8 53 358 1 344 1.71e-82 254
MS.gene066193.t1 AT1G05300 50.207 241 108 4 24 257 24 259 3.38e-74 231
MS.gene066193.t1 AT2G30080 39.710 345 188 8 28 358 3 341 1.15e-72 229
MS.gene066193.t1 AT4G19680 41.245 257 142 5 3 259 4 251 2.60e-59 192
MS.gene066193.t1 AT4G19690 40.541 148 88 0 5 152 8 155 1.07e-31 119
MS.gene066193.t1 AT1G60960 39.344 122 74 0 31 152 46 167 5.27e-26 106

Find 69 sgRNAs with CRISPR-Local

Find 176 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GTGAAATATTTGCTCCTATT+TGG 0.164008 2.2:-25396162 None:intergenic
CTTCGCGGCCGGTGTAATTT+TGG 0.178168 2.2:+25393631 MS.gene066193:CDS
CCTAATTAATTCAGGTGATA+TGG 0.183100 2.2:-25393948 None:intergenic
GGCCACTAGGAGAGGCTTTA+TGG 0.212975 2.2:-25394316 None:intergenic
ATAAGAAAATAAAAGCTTTA+AGG 0.213779 2.2:-25393390 None:intergenic
CCATATCACCTGAATTAATT+AGG 0.223925 2.2:+25393948 MS.gene066193:CDS
GTGATTATTGGAATATCTTT+AGG 0.251826 2.2:+25394273 MS.gene066193:CDS
GCATGTCCTTGTTGGCTAAA+TGG 0.255419 2.2:+25396203 MS.gene066193:CDS
ATCCTTTGCCTAATTAATTC+AGG 0.256019 2.2:-25393956 None:intergenic
GTCTAGGTCCACAGCAATTA+TGG 0.281046 2.2:+25395937 MS.gene066193:CDS
CTATCCCAATTGGTGTTGTT+AGG 0.295394 2.2:-25395973 None:intergenic
TCTGCTGGAACTTGTTTAGA+AGG 0.303586 2.2:-25393830 None:intergenic
TGTGGACCTAGACTCAAATT+TGG 0.329474 2.2:-25395927 None:intergenic
TTCTCCCTAACAACACCAAT+TGG 0.338474 2.2:+25395968 MS.gene066193:CDS
CATGTCCTTGTTGGCTAAAT+GGG 0.342112 2.2:+25396204 MS.gene066193:CDS
TTTGAATTCACCTTGTCTTA+AGG 0.344478 2.2:+25393694 MS.gene066193:CDS
AACAATTTCAAGATCCAAAT+AGG 0.351306 2.2:+25396148 MS.gene066193:CDS
GTCCTTGTTTGTGGTGCTCT+TGG 0.359872 2.2:+25393533 MS.gene066193:CDS
ATTGCTTCTGTCCTTGTTTG+TGG 0.360374 2.2:+25393524 MS.gene066193:CDS
ACACCAATTGGGATAGCAAT+TGG 0.365479 2.2:+25395980 MS.gene066193:CDS
GTTCCAGCAGATGAAGAAAT+AGG 0.370231 2.2:+25393842 MS.gene066193:CDS
GTCTTCTATAGCAACTTTGA+TGG 0.381420 2.2:+25393763 MS.gene066193:CDS
TTCAGGTTGCATGTCCTTGT+TGG 0.384055 2.2:+25396195 MS.gene066193:CDS
GTGGTCCATTCAGTGATTAT+TGG 0.385147 2.2:+25394261 MS.gene066193:CDS
TTTGAGGGAATGGGGCTTGG+TGG 0.391061 2.2:+25394357 MS.gene066193:CDS
TTGAACCAAGTTTGTAATGA+AGG 0.424018 2.2:-25393502 None:intergenic
CGGAAGTATGTGAATAAAAC+CGG 0.425664 2.2:-25393657 None:intergenic
AGCTGGAATCTTGATTTACA+TGG 0.436582 2.2:+25396078 MS.gene066193:CDS
CCAACATCTCTAATTGTTGA+AGG 0.439198 2.2:+25396037 MS.gene066193:CDS
GCTTGGTGGCTGCATATCTC+AGG 0.439946 2.2:+25394371 MS.gene066193:CDS
GAAGCCCTTCATTACAAACT+TGG 0.440871 2.2:+25393497 MS.gene066193:CDS
TCACCTATTTCTTCATCTGC+TGG 0.441100 2.2:-25393845 None:intergenic
TATCCCAATTGGTGTTGTTA+GGG 0.445987 2.2:-25395972 None:intergenic
ATCCCAATTGCTATCCCAAT+TGG 0.451924 2.2:-25395983 None:intergenic
CACCAATTGGGATAGCAATT+GGG 0.462103 2.2:+25395981 MS.gene066193:CDS
CTTCAACAATTAGAGATGTT+GGG 0.470274 2.2:-25396036 None:intergenic
TTCAAACTCTCAAATGCATC+CGG 0.473075 2.2:-25393677 None:intergenic
CCTTCAACAATTAGAGATGT+TGG 0.477232 2.2:-25396037 None:intergenic
GTGTATCAGGTATTGGAGCT+AGG 0.482364 2.2:+25394234 MS.gene066193:intron
ATGATTAAAGCCTTCGCGGC+CGG 0.485121 2.2:+25393620 MS.gene066193:CDS
TGAACCAAGTTTGTAATGAA+GGG 0.486936 2.2:-25393501 None:intergenic
TCTCCCTAACAACACCAATT+GGG 0.487085 2.2:+25395969 MS.gene066193:CDS
CAAAATTACACCGGCCGCGA+AGG 0.490438 2.2:-25393630 None:intergenic
GAATCGCATGAAACAAGAGT+TGG 0.514536 2.2:-25393428 None:intergenic
GCCACACATGGTCATGCTCA+TGG 0.524602 2.2:+25393902 MS.gene066193:CDS
GGTATTGGAGCTAGGAATAG+TGG 0.526265 2.2:+25394242 MS.gene066193:intron
TTTCATGATTAAAGCCTTCG+CGG 0.533059 2.2:+25393616 MS.gene066193:CDS
AATCGCATGAAACAAGAGTT+GGG 0.534049 2.2:-25393427 None:intergenic
ATATTCCAATAATCACTGAA+TGG 0.557063 2.2:-25394266 None:intergenic
TGCAGGCCAAATTTGAGTCT+AGG 0.558044 2.2:+25395921 MS.gene066193:intron
TCATAAAATCAGCTGCAAGT+AGG 0.577409 2.2:-25396111 None:intergenic
CACCTGAATTAATTAGGCAA+AGG 0.578421 2.2:+25393954 MS.gene066193:CDS
GATGGAAAGACAAGGCCACT+AGG 0.600701 2.2:-25394329 None:intergenic
AATTGGGATAGCAATTGGGA+TGG 0.606135 2.2:+25395985 MS.gene066193:CDS
AAAGACAAGGCCACTAGGAG+AGG 0.616440 2.2:-25394324 None:intergenic
CACCAAGAGCACCACAAACA+AGG 0.617962 2.2:-25393535 None:intergenic
ATAGCAACAAGTCCAGCAAG+TGG 0.627097 2.2:-25393737 None:intergenic
ATTGGGATAGCAATTGGGAT+GGG 0.627940 2.2:+25395986 MS.gene066193:CDS
GTTCATACACATGCCACACA+TGG 0.634479 2.2:+25393890 MS.gene066193:CDS
TGATATGGAATCTTGAGAAG+AGG 0.635195 2.2:-25393933 None:intergenic
AAATGTTGCCATAATTGCTG+TGG 0.645802 2.2:-25395945 None:intergenic
ACCGGTTGCCAAAATTACAC+CGG 0.652557 2.2:-25393639 None:intergenic
GAAATAGGTGATGATCATGT+TGG 0.666063 2.2:+25393857 MS.gene066193:CDS
TACCATAAAGCCTCTCCTAG+TGG 0.667478 2.2:+25394314 MS.gene066193:CDS
TGTGAAACTGAACAAACCAA+AGG 0.668514 2.2:+25393458 MS.gene066193:CDS
GCAAGTGGAAAATCACCCCA+AGG 0.669491 2.2:-25393722 None:intergenic
TTATGCCCATTTAGCCAACA+AGG 0.682180 2.2:-25396209 None:intergenic
TCCATGAGCATGACCATGTG+TGG 0.697142 2.2:-25393903 None:intergenic
CGCATGAAACAAGAGTTGGG+AGG 0.742798 2.2:-25393424 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AATATTTGGTAAAATAAAAT+TGG - chr2.2:25394543-25394562 None:intergenic 10.0%
!! GTTAAAAATATTATTACATT+TGG - chr2.2:25395442-25395461 None:intergenic 10.0%
!! AAAAAACCAATATTATATGT+CGG - chr2.2:25395523-25395542 None:intergenic 15.0%
!! AAAAATTTAAACACTCAAAT+AGG + chr2.2:25395192-25395211 MS.gene066193:intron 15.0%
!! ATAAGAAAATAAAAGCTTTA+AGG - chr2.2:25393393-25393412 None:intergenic 15.0%
!! TGAAAGTAAAAGAAATAAAT+TGG - chr2.2:25394667-25394686 None:intergenic 15.0%
!! TTGCAAAATTAAATCATATA+AGG + chr2.2:25394710-25394729 MS.gene066193:intron 15.0%
!!! AATCTAAATTCTTTTTTTAG+TGG + chr2.2:25394606-25394625 MS.gene066193:intron 15.0%
!!! ATGAAAAATATGTCATTTTT+AGG - chr2.2:25393602-25393621 None:intergenic 15.0%
!!! GTTTTTTGTAAAAAAAAATG+TGG + chr2.2:25395039-25395058 MS.gene066193:intron 15.0%
!!! TGAAAAATATGTCATTTTTA+GGG - chr2.2:25393601-25393620 None:intergenic 15.0%
!! AACCAAATTGAATATTAATC+GGG - chr2.2:25394207-25394226 None:intergenic 20.0%
!! AATAACGATTACGTAATTAT+TGG - chr2.2:25394115-25394134 None:intergenic 20.0%
!! AATTATAACATATTATGTGC+AGG + chr2.2:25395904-25395923 MS.gene066193:intron 20.0%
!! ACAATTTGGACTATAATATT+TGG - chr2.2:25394557-25394576 None:intergenic 20.0%
!! CTCAAGTAATTAATAAGTTT+CGG - chr2.2:25394144-25394163 None:intergenic 20.0%
!! GAACCAAATTGAATATTAAT+CGG - chr2.2:25394208-25394227 None:intergenic 20.0%
!! GACAATTTAAATTCAGATTT+AGG + chr2.2:25394460-25394479 MS.gene066193:intron 20.0%
!! TAATCATTTAAGTAAAGTCT+CGG - chr2.2:25395792-25395811 None:intergenic 20.0%
!! TATAGTTAAACTTCCTAAAA+TGG + chr2.2:25394758-25394777 MS.gene066193:intron 20.0%
!! TTAGATAAAAAACACTAACT+CGG + chr2.2:25395478-25395497 MS.gene066193:intron 20.0%
!!! ATATCTAACTTTAGAACTAT+TGG - chr2.2:25394981-25395000 None:intergenic 20.0%
!!! ATTTTTATGGGACTAAATTA+GGG - chr2.2:25395066-25395085 None:intergenic 20.0%
!!! ATTTTTTTCACTATTTCCTT+TGG - chr2.2:25393477-25393496 None:intergenic 20.0%
!!! CAATTTACAGACATTTTTAT+GGG - chr2.2:25395078-25395097 None:intergenic 20.0%
!!! GAGTGTTTAAATTTTTGTTA+AGG - chr2.2:25395188-25395207 None:intergenic 20.0%
!!! TAAATAATGAACAAGTCTTT+TGG - chr2.2:25394167-25394186 None:intergenic 20.0%
!!! TCAATTTACAGACATTTTTA+TGG - chr2.2:25395079-25395098 None:intergenic 20.0%
!!! TTTACAAGGTTTTAATACTT+CGG + chr2.2:25395290-25395309 MS.gene066193:intron 20.0%
! AAAAAATTGATGGAAAGACA+AGG - chr2.2:25394340-25394359 None:intergenic 25.0%
! AACAATTTCAAGATCCAAAT+AGG + chr2.2:25396148-25396167 MS.gene066193:CDS 25.0%
! AATCATTTAAGTAAAGTCTC+GGG - chr2.2:25395791-25395810 None:intergenic 25.0%
! ACTTTGCTGATAATTAATCT+CGG + chr2.2:25395685-25395704 MS.gene066193:intron 25.0%
! ATATTCCAATAATCACTGAA+TGG - chr2.2:25394269-25394288 None:intergenic 25.0%
! ATTAATTATCAGCAAAGTAG+AGG - chr2.2:25395683-25395702 None:intergenic 25.0%
! GTGATTATTGGAATATCTTT+AGG + chr2.2:25394273-25394292 MS.gene066193:CDS 25.0%
! TACCCGATTAATATTCAATT+TGG + chr2.2:25394202-25394221 MS.gene066193:intron 25.0%
! TGATTCTACATTTATTAGAG+AGG + chr2.2:25395621-25395640 MS.gene066193:intron 25.0%
! TTAATTATCAGCAAAGTAGA+GGG - chr2.2:25395682-25395701 None:intergenic 25.0%
!! ACTTGAAACTTTTCCATTTT+AGG - chr2.2:25394774-25394793 None:intergenic 25.0%
!! CATTTTTATGGGACTAAATT+AGG - chr2.2:25395067-25395086 None:intergenic 25.0%
!! GCAAATATTTCACTTCTTTT+AGG + chr2.2:25396172-25396191 MS.gene066193:CDS 25.0%
!! TTTTTATGGGACTAAATTAG+GGG - chr2.2:25395065-25395084 None:intergenic 25.0%
!!! TCTTTCCATCAATTTTTTGA+GGG + chr2.2:25394342-25394361 MS.gene066193:CDS 25.0%
!!! TGAGATATTGTCTTTTTTCT+TGG - chr2.2:25395220-25395239 None:intergenic 25.0%
AAAAAAATTAGCCATGCGAT+TGG + chr2.2:25395647-25395666 MS.gene066193:intron 30.0%
AAATTCAATACCAACCCATA+AGG - chr2.2:25394643-25394662 None:intergenic 30.0%
AATTCAATACCAACCCATAA+GGG - chr2.2:25394642-25394661 None:intergenic 30.0%
ATCCTTTGCCTAATTAATTC+AGG - chr2.2:25393959-25393978 None:intergenic 30.0%
CAATAAGGCAATTGACAATT+TGG - chr2.2:25394571-25394590 None:intergenic 30.0%
CCATATCACCTGAATTAATT+AGG + chr2.2:25393948-25393967 MS.gene066193:CDS 30.0%
CCTAATTAATTCAGGTGATA+TGG - chr2.2:25393951-25393970 None:intergenic 30.0%
CTTCAACAATTAGAGATGTT+GGG - chr2.2:25396039-25396058 None:intergenic 30.0%
GTATTGTAGATGAAAAAGTG+TGG - chr2.2:25395763-25395782 None:intergenic 30.0%
GTGAAATATTTGCTCCTATT+TGG - chr2.2:25396165-25396184 None:intergenic 30.0%
TAGAACTATTGGACTCATAT+AGG - chr2.2:25394970-25394989 None:intergenic 30.0%
TGAACCAAGTTTGTAATGAA+GGG - chr2.2:25393504-25393523 None:intergenic 30.0%
TTGAACCAAGTTTGTAATGA+AGG - chr2.2:25393505-25393524 None:intergenic 30.0%
TTGTCTTAAGGAAAAACCTT+GGG + chr2.2:25393706-25393725 MS.gene066193:CDS 30.0%
TTTGAATTCACCTTGTCTTA+AGG + chr2.2:25393694-25393713 MS.gene066193:CDS 30.0%
! CTATTTTGTGTATCAGGTAT+TGG + chr2.2:25394227-25394246 MS.gene066193:intron 30.0%
! GGAATTCTTTTGCTCAATAA+TGG - chr2.2:25393569-25393588 None:intergenic 30.0%
! GTTTCTAAGAGTCAATTTAC+AGG - chr2.2:25395144-25395163 None:intergenic 30.0%
! TCAATTCACTAGTGACTTTT+AGG - chr2.2:25394934-25394953 None:intergenic 30.0%
!! GATTTAATTTTGCAACAACG+AGG - chr2.2:25394705-25394724 None:intergenic 30.0%
!! GTTAATTTGTACTACTGAGT+AGG - chr2.2:25394881-25394900 None:intergenic 30.0%
!! TTCTTTTGCTCAATAATGGA+AGG - chr2.2:25393565-25393584 None:intergenic 30.0%
!! TTGAAATTGTTTTGCATCCT+TGG - chr2.2:25396139-25396158 None:intergenic 30.0%
!!! ATCAATTTTTTGAGGGAATG+GGG + chr2.2:25394349-25394368 MS.gene066193:CDS 30.0%
!!! ATTTCACTTCTTTTAGGTTC+AGG + chr2.2:25396178-25396197 MS.gene066193:CDS 30.0%
!!! CATCAATTTTTTGAGGGAAT+GGG + chr2.2:25394348-25394367 MS.gene066193:CDS 30.0%
!!! GTCTTTCCATCAATTTTTTG+AGG + chr2.2:25394341-25394360 MS.gene066193:CDS 30.0%
!!! TCATTTTTAGGGCTTAGAAT+TGG - chr2.2:25393590-25393609 None:intergenic 30.0%
!!! TTACGGAGTCTTTATTTTCA+TGG - chr2.2:25394058-25394077 None:intergenic 30.0%
!!! TTGGTTCTATTTTGTGTATC+AGG + chr2.2:25394221-25394240 MS.gene066193:intron 30.0%
AAATGTTGCCATAATTGCTG+TGG - chr2.2:25395948-25395967 None:intergenic 35.0%
AACTCGGAGTGTATTGTATA+TGG + chr2.2:25395494-25395513 MS.gene066193:intron 35.0%
AATCGCATGAAACAAGAGTT+GGG - chr2.2:25393430-25393449 None:intergenic 35.0%
AATTTGTACTACTGAGTAGG+AGG - chr2.2:25394878-25394897 None:intergenic 35.0%
ACTATTGCAGTTTGGAAAGT+AGG + chr2.2:25394823-25394842 MS.gene066193:intron 35.0%
AGCTGGAATCTTGATTTACA+TGG + chr2.2:25396078-25396097 MS.gene066193:CDS 35.0%
CAATATTACATTAGTCCGAG+AGG - chr2.2:25395825-25395844 None:intergenic 35.0%
CACCTGAATTAATTAGGCAA+AGG + chr2.2:25393954-25393973 MS.gene066193:CDS 35.0%
CCAACATCTCTAATTGTTGA+AGG + chr2.2:25396037-25396056 MS.gene066193:CDS 35.0%
CCATTCCCTCAAAAAATTGA+TGG - chr2.2:25394350-25394369 None:intergenic 35.0%
CCTTCAACAATTAGAGATGT+TGG - chr2.2:25396040-25396059 None:intergenic 35.0%
CTATGCTTTCTCACACAATA+AGG - chr2.2:25394586-25394605 None:intergenic 35.0%
CTCTCGGACTAATGTAATAT+TGG + chr2.2:25395823-25395842 MS.gene066193:intron 35.0%
CTTAAATTTCAACCGAGCTT+TGG - chr2.2:25394082-25394101 None:intergenic 35.0%
CTTGTCTTAAGGAAAAACCT+TGG + chr2.2:25393705-25393724 MS.gene066193:CDS 35.0%
GAAATAGGTGATGATCATGT+TGG + chr2.2:25393857-25393876 MS.gene066193:CDS 35.0%
GTCTTCTATAGCAACTTTGA+TGG + chr2.2:25393763-25393782 MS.gene066193:CDS 35.0%
TAAAACCTTGTAAACCGTGT+CGG - chr2.2:25395284-25395303 None:intergenic 35.0%
TCATAAAATCAGCTGCAAGT+AGG - chr2.2:25396114-25396133 None:intergenic 35.0%
TGATATGGAATCTTGAGAAG+AGG - chr2.2:25393936-25393955 None:intergenic 35.0%
TGGATGTTACTATTGCAGTT+TGG + chr2.2:25394815-25394834 MS.gene066193:intron 35.0%
TGGTGTCCGACATATAATAT+TGG + chr2.2:25395514-25395533 MS.gene066193:intron 35.0%
TGTCTTAAGGAAAAACCTTG+GGG + chr2.2:25393707-25393726 MS.gene066193:CDS 35.0%
TGTGAAACTGAACAAACCAA+AGG + chr2.2:25393458-25393477 MS.gene066193:CDS 35.0%
TTAATCTCGGTAAGAGATCT+TGG + chr2.2:25395698-25395717 MS.gene066193:intron 35.0%
TTCAAACTCTCAAATGCATC+CGG - chr2.2:25393680-25393699 None:intergenic 35.0%
TTTAGGTCTTTCTCTCTTCA+TGG - chr2.2:25394917-25394936 None:intergenic 35.0%
TTTCATGATTAAAGCCTTCG+CGG + chr2.2:25393616-25393635 MS.gene066193:CDS 35.0%
! CAAGGTTTTTCCTTAAGACA+AGG - chr2.2:25393707-25393726 None:intergenic 35.0%
! CGGAAGTATGTGAATAAAAC+CGG - chr2.2:25393660-25393679 None:intergenic 35.0%
! GATTAGAGTTTAGTTCCTCT+CGG + chr2.2:25395807-25395826 MS.gene066193:intron 35.0%
!! TAACCATTGACCACTTTTAG+TGG + chr2.2:25395722-25395741 MS.gene066193:intron 35.0%
!! TATCCCAATTGGTGTTGTTA+GGG - chr2.2:25395975-25395994 None:intergenic 35.0%
!!! CCATCAATTTTTTGAGGGAA+TGG + chr2.2:25394347-25394366 MS.gene066193:CDS 35.0%
!!! CGGTTTTATTCACATACTTC+CGG + chr2.2:25393658-25393677 MS.gene066193:CDS 35.0%
AAGACAGACTCCACTAAAAG+TGG - chr2.2:25395735-25395754 None:intergenic 40.0%
AATTGGGATAGCAATTGGGA+TGG + chr2.2:25395985-25396004 MS.gene066193:CDS 40.0%
ACACCAATTGGGATAGCAAT+TGG + chr2.2:25395980-25395999 MS.gene066193:CDS 40.0%
ACTCCACTAAAAGTGGTCAA+TGG - chr2.2:25395728-25395747 None:intergenic 40.0%
ATCCCAATTGCTATCCCAAT+TGG - chr2.2:25395986-25396005 None:intergenic 40.0%
ATTGGGATAGCAATTGGGAT+GGG + chr2.2:25395986-25396005 MS.gene066193:CDS 40.0%
CAAGAAAAGTGAGTTGCATG+TGG + chr2.2:25394795-25394814 MS.gene066193:intron 40.0%
CACCAATTGGGATAGCAATT+GGG + chr2.2:25395981-25396000 MS.gene066193:CDS 40.0%
CATAGTCACACAATTTGAGC+AGG + chr2.2:25394424-25394443 MS.gene066193:intron 40.0%
GAAGCCCTTCATTACAAACT+TGG + chr2.2:25393497-25393516 MS.gene066193:CDS 40.0%
GAATCGCATGAAACAAGAGT+TGG - chr2.2:25393431-25393450 None:intergenic 40.0%
GTGGTCCATTCAGTGATTAT+TGG + chr2.2:25394261-25394280 MS.gene066193:CDS 40.0%
GTTCCAGCAGATGAAGAAAT+AGG + chr2.2:25393842-25393861 MS.gene066193:CDS 40.0%
TCTCCCTAACAACACCAATT+GGG + chr2.2:25395969-25395988 MS.gene066193:CDS 40.0%
TGGATATGCAAGACCCTTAT+GGG + chr2.2:25394626-25394645 MS.gene066193:intron 40.0%
TGTGGACCTAGACTCAAATT+TGG - chr2.2:25395930-25395949 None:intergenic 40.0%
TTATGCCCATTTAGCCAACA+AGG - chr2.2:25396212-25396231 None:intergenic 40.0%
TTCTCCCTAACAACACCAAT+TGG + chr2.2:25395968-25395987 MS.gene066193:CDS 40.0%
! ATTGCTTCTGTCCTTGTTTG+TGG + chr2.2:25393524-25393543 MS.gene066193:CDS 40.0%
! CATGTCCTTGTTGGCTAAAT+GGG + chr2.2:25396204-25396223 MS.gene066193:CDS 40.0%
! GTTTTCAATTCTGCTTCAGC+TGG + chr2.2:25396061-25396080 MS.gene066193:CDS 40.0%
! TCACCTATTTCTTCATCTGC+TGG - chr2.2:25393848-25393867 None:intergenic 40.0%
! TCTGCTGGAACTTGTTTAGA+AGG - chr2.2:25393833-25393852 None:intergenic 40.0%
!! CAGCTGATTTTATGAGTCCA+AGG + chr2.2:25396119-25396138 MS.gene066193:CDS 40.0%
!! CTATCCCAATTGGTGTTGTT+AGG - chr2.2:25395976-25395995 None:intergenic 40.0%
!! TTCAACCGAGCTTTGGATTA+CGG - chr2.2:25394075-25394094 None:intergenic 40.0%
ACCGGTTGCCAAAATTACAC+CGG - chr2.2:25393642-25393661 None:intergenic 45.0%
AGACTCCGTAATCCAAAGCT+CGG + chr2.2:25394067-25394086 MS.gene066193:intron 45.0%
ATAGCAACAAGTCCAGCAAG+TGG - chr2.2:25393740-25393759 None:intergenic 45.0%
GTAGGAGGATGTCGTCATAA+TGG - chr2.2:25394863-25394882 None:intergenic 45.0%
GTCTAGGTCCACAGCAATTA+TGG + chr2.2:25395937-25395956 MS.gene066193:CDS 45.0%
GTGGATATGCAAGACCCTTA+TGG + chr2.2:25394625-25394644 MS.gene066193:intron 45.0%
GTTCATACACATGCCACACA+TGG + chr2.2:25393890-25393909 MS.gene066193:CDS 45.0%
TACCAACCGAACTTAGCTCA+CGG + chr2.2:25395116-25395135 MS.gene066193:intron 45.0%
TACCATAAAGCCTCTCCTAG+TGG + chr2.2:25394314-25394333 MS.gene066193:CDS 45.0%
TATGCAAGACCCTTATGGGT+TGG + chr2.2:25394630-25394649 MS.gene066193:intron 45.0%
TGCAGGCCAAATTTGAGTCT+AGG + chr2.2:25395921-25395940 MS.gene066193:intron 45.0%
! GCATGTCCTTGTTGGCTAAA+TGG + chr2.2:25396203-25396222 MS.gene066193:CDS 45.0%
! GGTATTGGAGCTAGGAATAG+TGG + chr2.2:25394242-25394261 MS.gene066193:intron 45.0%
! TGAGCTAAGTTCGGTTGGTA+AGG - chr2.2:25395116-25395135 None:intergenic 45.0%
!! GTGTATCAGGTATTGGAGCT+AGG + chr2.2:25394234-25394253 MS.gene066193:intron 45.0%
!! TTCAGGTTGCATGTCCTTGT+TGG + chr2.2:25396195-25396214 MS.gene066193:CDS 45.0%
!!! TTTTTTGAGGGAATGGGGCT+TGG + chr2.2:25394354-25394373 MS.gene066193:CDS 45.0%
AAACATCCTGACAGCCGACA+CGG + chr2.2:25395267-25395286 MS.gene066193:intron 50.0%
AAAGACAAGGCCACTAGGAG+AGG - chr2.2:25394327-25394346 None:intergenic 50.0%
ACCAACCGAACTTAGCTCAC+GGG + chr2.2:25395117-25395136 MS.gene066193:intron 50.0%
ATGATTAAAGCCTTCGCGGC+CGG + chr2.2:25393620-25393639 MS.gene066193:CDS 50.0%
CGCATGAAACAAGAGTTGGG+AGG - chr2.2:25393427-25393446 None:intergenic 50.0%
GATGGAAAGACAAGGCCACT+AGG - chr2.2:25394332-25394351 None:intergenic 50.0%
GCAAGTGGAAAATCACCCCA+AGG - chr2.2:25393725-25393744 None:intergenic 50.0%
GGTACTTCACACCAATCGCA+TGG - chr2.2:25395661-25395680 None:intergenic 50.0%
TCCATGAGCATGACCATGTG+TGG - chr2.2:25393906-25393925 None:intergenic 50.0%
! GTCCTTGTTTGTGGTGCTCT+TGG + chr2.2:25393533-25393552 MS.gene066193:CDS 50.0%
! TGGGGTGATTTTCCACTTGC+TGG + chr2.2:25393725-25393744 MS.gene066193:CDS 50.0%
!! CACCAAGAGCACCACAAACA+AGG - chr2.2:25393538-25393557 None:intergenic 50.0%
!! GCCGGTGTAATTTTGGCAAC+CGG + chr2.2:25393638-25393657 MS.gene066193:CDS 50.0%
CAAAATTACACCGGCCGCGA+AGG - chr2.2:25393633-25393652 None:intergenic 55.0%
CAGGTCCCGTGAGCTAAGTT+CGG - chr2.2:25395125-25395144 None:intergenic 55.0%
GACAGCCGACACGGTTTACA+AGG + chr2.2:25395276-25395295 MS.gene066193:intron 55.0%
GCCACACATGGTCATGCTCA+TGG + chr2.2:25393902-25393921 MS.gene066193:CDS 55.0%
GGCCACTAGGAGAGGCTTTA+TGG - chr2.2:25394319-25394338 None:intergenic 55.0%
TCCCGTGAGCTAAGTTCGGT+TGG - chr2.2:25395121-25395140 None:intergenic 55.0%
TTTGAGGGAATGGGGCTTGG+TGG + chr2.2:25394357-25394376 MS.gene066193:CDS 55.0%
! CTTCGCGGCCGGTGTAATTT+TGG + chr2.2:25393631-25393650 MS.gene066193:CDS 55.0%
! GCTTGGTGGCTGCATATCTC+AGG + chr2.2:25394371-25394390 MS.gene066193:CDS 55.0%
!! TGTAAACCGTGTCGGCTGTC+AGG - chr2.2:25395276-25395295 None:intergenic 55.0%


Chromosome Type Strat End Strand Name
chr2.2 gene 25393368 25396231 25393368 ID=MS.gene066193
chr2.2 mRNA 25393368 25396231 25393368 ID=MS.gene066193.t1;Parent=MS.gene066193
chr2.2 exon 25393368 25393988 25393368 ID=MS.gene066193.t1.exon1;Parent=MS.gene066193.t1
chr2.2 CDS 25393368 25393988 25393368 ID=cds.MS.gene066193.t1;Parent=MS.gene066193.t1
chr2.2 exon 25394243 25394392 25394243 ID=MS.gene066193.t1.exon2;Parent=MS.gene066193.t1
chr2.2 CDS 25394243 25394392 25394243 ID=cds.MS.gene066193.t1;Parent=MS.gene066193.t1
chr2.2 exon 25395926 25396231 25395926 ID=MS.gene066193.t1.exon3;Parent=MS.gene066193.t1
chr2.2 CDS 25395926 25396231 25395926 ID=cds.MS.gene066193.t1;Parent=MS.gene066193.t1
Gene Sequence

>MS.gene066193

ATGATGAGTTCTCTTACTACTACCTTAAAGCTTTTATTTTCTTATGTGATTTTTGTCCTCCCAACTCTTGTTTCATGCGATTCATGCACATGTGAAACTGAACAAACCAAAGGAAATAGTGAAAAAAATGAAGCCCTTCATTACAAACTTGGTTCAATTGCTTCTGTCCTTGTTTGTGGTGCTCTTGGTGTAAGCCTTCCATTATTGAGCAAAAGAATTCCAATTCTAAGCCCTAAAAATGACATATTTTTCATGATTAAAGCCTTCGCGGCCGGTGTAATTTTGGCAACCGGTTTTATTCACATACTTCCGGATGCATTTGAGAGTTTGAATTCACCTTGTCTTAAGGAAAAACCTTGGGGTGATTTTCCACTTGCTGGACTTGTTGCTATGTTGTCTTCTATAGCAACTTTGATGGTTGATTCATTTGCATCTAGCTATTATCAAAAGAGACATTTTAATCCTTCTAAACAAGTTCCAGCAGATGAAGAAATAGGTGATGATCATGTTGGTCACATACATGTTCATACACATGCCACACATGGTCATGCTCATGGATCAGCTACCTCTTCTCAAGATTCCATATCACCTGAATTAATTAGGCAAAGGATCATATCACAAGTAAGCTCACTGAATTGTTTCTATGTGCAAAAATAGATACATGCATTTTTTTTTTTGTTTAACTCTCCATGAAAATAAAGACTCCGTAATCCAAAGCTCGGTTGAAATTTAAGTAAAGTTTAACCAATAATTACGTAATCGTTATTAATTAACCGAAACTTATTAATTACTTGAGCCAAAAGACTTGTTCATTATTTAATTTTTTTATTTGTGTACCCGATTAATATTCAATTTGGTTCTATTTTGTGTATCAGGTATTGGAGCTAGGAATAGTGGTCCATTCAGTGATTATTGGAATATCTTTAGGAACTGCACAAAGCATTGATACCATAAAGCCTCTCCTAGTGGCCTTGTCTTTCCATCAATTTTTTGAGGGAATGGGGCTTGGTGGCTGCATATCTCAGGTATTTTAAGAGTTTCTTTTTATACATTTAACATAGTCACACAATTTGAGCAGGTAGATCAAGATCAGACAATTTAAATTCAGATTTAGGTGAATTAGATTTAAAATACAAAGTTAGAAAATGAGTTGTTTTGAAATTAATTATAGTCCAATTTTATTTTACCAAATATTATAGTCCAAATTGTCAATTGCCTTATTGTGTGAGAAAGCATAGAATCTAAATTCTTTTTTTAGTGGATATGCAAGACCCTTATGGGTTGGTATTGAATTTTCCAATTTATTTCTTTTACTTTCAACGTTTGCAATTCTTCCTCGTTGTTGCAAAATTAAATCATATAAGGTATTAAGAAAGTGAAGAAATGAAGCTATAGTTAAACTTCCTAAAATGGAAAAGTTTCAAGTTCAAGAAAAGTGAGTTGCATGTGGATGTTACTATTGCAGTTTGGAAAGTAGGTTTGCAAAATGTTGCCATTATGACGACATCCTCCTACTCAGTAGTACAAATTAACAAAACTTCTTACTCCATGAAGAGAGAAAGACCTAAAAGTCACTAGTGAATTGAGCTGTTTGTTCATCCTATATGAGTCCAATAGTTCTAAAGTTAGATATAAGAATAAAATAAAAATCAAGACTAAAGACTCATTTTAGTTTTTTGTAAAAAAAAATGTGGCCCCTAATTTAGTCCCATAAAAATGTCTGTAAATTGACTCTTATGCATTGTCCTTACCAACCGAACTTAGCTCACGGGACCTGTAAATTGACTCTTAGAAACAATCTATCATGACATACTAGTCCTTAACAAAAATTTAAACACTCAAATAGGTACCAAGAAAAAAGACAATATCTCATGATTTTATAATTGTAAATATTTTTTTAAACATCCTGACAGCCGACACGGTTTACAAGGTTTTAATACTTCGGACTTATTTACAAGTGAAAGTTATCTTTTTAGTTTCATTCATAAATGATGTATCTAGTCTACGTGAAGATTTAGATACATCATTTATTAAATAAATCTAAAAAGTAAACTTTCTCTTATAAATGAGACCAAATGTAATAATATTTTTAACAATATTTCTTTTCTTCTTAGATAAAAAACACTAACTCGGAGTGTATTGTATATGGTGTCCGACATATAATATTGGTTTTTTGTGTCAAAATAACGATTCTCATAATTAATTTTGCTAGCTTAGCTTATTTGTAATTTTCTCTACATGCTCAATTATATTTGATTCTACATTTATTAGAGAGGTTAAAAAAAATTAGCCATGCGATTGGTGTGAAGTACCCTCTACTTTGCTGATAATTAATCTCGGTAAGAGATCTTGGCTAACCATTGACCACTTTTAGTGGAGTCTGTCTTGTCAACCACACTTTTTCATCTACAATACTTGAACCCGAGACTTTACTTAAATGATTAGAGTTTAGTTCCTCTCGGACTAATGTAATATTGGTGATTAAAATCAGTATTTACTAAAAGCTAACAATAATTTGTGTATGTGTTTAGTTACTAATTATAACATATTATGTGCAGGCCAAATTTGAGTCTAGGTCCACAGCAATTATGGCAACATTTTTCTCCCTAACAACACCAATTGGGATAGCAATTGGGATGGGAGTATCAAGTGTGTACAAAGACAATAGCCCAACATCTCTAATTGTTGAAGGTGTTTTCAATTCTGCTTCAGCTGGAATCTTGATTTACATGGCACTAGTTGACCTACTTGCAGCTGATTTTATGAGTCCAAGGATGCAAAACAATTTCAAGATCCAAATAGGAGCAAATATTTCACTTCTTTTAGGTTCAGGTTGCATGTCCTTGTTGGCTAAATGGGCATAA
Protein sequence

>MS.gene066193.t1

MMSSLTTTLKLLFSYVIFVLPTLVSCDSCTCETEQTKGNSEKNEALHYKLGSIASVLVCGALGVSLPLLSKRIPILSPKNDIFFMIKAFAAGVILATGFIHILPDAFESLNSPCLKEKPWGDFPLAGLVAMLSSIATLMVDSFASSYYQKRHFNPSKQVPADEEIGDDHVGHIHVHTHATHGHAHGSATSSQDSISPELIRQRIISQVLELGIVVHSVIIGISLGTAQSIDTIKPLLVALSFHQFFEGMGLGGCISQAKFESRSTAIMATFFSLTTPIGIAIGMGVSSVYKDNSPTSLIVEGVFNSASAGILIYMALVDLLAADFMSPRMQNNFKIQIGANISLLLGSGCMSLLAKWA