Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene066718.t1 | XP_003608251.2 | 98.1 | 154 | 3 | 0 | 1 | 154 | 20 | 173 | 1.80E-82 | 315.1 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene066718.t1 | Q570C0 | 61.5 | 148 | 57 | 0 | 7 | 154 | 9 | 156 | 3.2e-51 | 202.6 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene066718.t1 | G7JRV7 | 98.1 | 154 | 3 | 0 | 1 | 154 | 20 | 173 | 1.3e-82 | 315.1 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049142 | MS.gene066718 | 0.814883 | 1.23E-51 | -1.69E-46 |
| MS.gene049283 | MS.gene066718 | 0.811524 | 6.71E-51 | -1.69E-46 |
| MS.gene049983 | MS.gene066718 | 0.806354 | 8.57E-50 | -1.69E-46 |
| MS.gene050084 | MS.gene066718 | 0.804258 | 2.36E-49 | -1.69E-46 |
| MS.gene050085 | MS.gene066718 | 0.814938 | 1.19E-51 | -1.69E-46 |
| MS.gene050359 | MS.gene066718 | 0.802208 | 6.26E-49 | -1.69E-46 |
| MS.gene050361 | MS.gene066718 | 0.807615 | 4.64E-50 | -1.69E-46 |
| MS.gene050384 | MS.gene066718 | 0.808131 | 3.60E-50 | -1.69E-46 |
| MS.gene050386 | MS.gene066718 | 0.805459 | 1.32E-49 | -1.69E-46 |
| MS.gene050414 | MS.gene066718 | 0.814234 | 1.71E-51 | -1.69E-46 |
| MS.gene050646 | MS.gene066718 | 0.823147 | 1.62E-53 | -1.69E-46 |
| MS.gene050654 | MS.gene066718 | 0.812978 | 3.23E-51 | -1.69E-46 |
| MS.gene050689 | MS.gene066718 | 0.80755 | 4.79E-50 | -1.69E-46 |
| MS.gene050807 | MS.gene066718 | 0.835045 | 2.11E-56 | -1.69E-46 |
| MS.gene051102 | MS.gene066718 | 0.801575 | 8.44E-49 | -1.69E-46 |
| MS.gene051198 | MS.gene066718 | 0.813773 | 2.16E-51 | -1.69E-46 |
| MS.gene051233 | MS.gene066718 | 0.800995 | 1.11E-48 | -1.69E-46 |
| MS.gene051343 | MS.gene066718 | 0.83453 | 2.84E-56 | -1.69E-46 |
| MS.gene051856 | MS.gene066718 | 0.810645 | 1.04E-50 | -1.69E-46 |
| MS.gene051902 | MS.gene066718 | 0.809839 | 1.55E-50 | -1.69E-46 |
| MS.gene052239 | MS.gene066718 | -0.810943 | 8.96E-51 | -1.69E-46 |
| MS.gene052242 | MS.gene066718 | -0.830408 | 2.99E-55 | -1.69E-46 |
| MS.gene052307 | MS.gene066718 | 0.828189 | 1.03E-54 | -1.69E-46 |
| MS.gene052609 | MS.gene066718 | 0.836497 | 9.04E-57 | -1.69E-46 |
| MS.gene052776 | MS.gene066718 | 0.805455 | 1.32E-49 | -1.69E-46 |
| MS.gene052994 | MS.gene066718 | 0.800402 | 1.47E-48 | -1.69E-46 |
| MS.gene053079 | MS.gene066718 | 0.827679 | 1.37E-54 | -1.69E-46 |
| MS.gene053143 | MS.gene066718 | 0.801679 | 8.04E-49 | -1.69E-46 |
| MS.gene053189 | MS.gene066718 | 0.804548 | 2.05E-49 | -1.69E-46 |
| MS.gene053563 | MS.gene066718 | 0.805667 | 1.20E-49 | -1.69E-46 |
| MS.gene053590 | MS.gene066718 | 0.807527 | 4.84E-50 | -1.69E-46 |
| MS.gene05364 | MS.gene066718 | 0.818949 | 1.50E-52 | -1.69E-46 |
| MS.gene053931 | MS.gene066718 | 0.803603 | 3.22E-49 | -1.69E-46 |
| MS.gene055311 | MS.gene066718 | 0.815697 | 8.10E-52 | -1.69E-46 |
| MS.gene055313 | MS.gene066718 | 0.801991 | 6.93E-49 | -1.69E-46 |
| MS.gene056045 | MS.gene066718 | 0.851841 | 6.82E-61 | -1.69E-46 |
| MS.gene056181 | MS.gene066718 | 0.819522 | 1.11E-52 | -1.69E-46 |
| MS.gene056308 | MS.gene066718 | 0.828355 | 9.42E-55 | -1.69E-46 |
| MS.gene056507 | MS.gene066718 | 0.80697 | 6.35E-50 | -1.69E-46 |
| MS.gene056578 | MS.gene066718 | 0.821209 | 4.56E-53 | -1.69E-46 |
| MS.gene057919 | MS.gene066718 | 0.805628 | 1.22E-49 | -1.69E-46 |
| MS.gene058010 | MS.gene066718 | 0.801452 | 8.94E-49 | -1.69E-46 |
| MS.gene058054 | MS.gene066718 | 0.811414 | 7.09E-51 | -1.69E-46 |
| MS.gene058292 | MS.gene066718 | 0.83015 | 3.45E-55 | -1.69E-46 |
| MS.gene058330 | MS.gene066718 | 0.811972 | 5.36E-51 | -1.69E-46 |
| MS.gene058638 | MS.gene066718 | 0.844287 | 8.30E-59 | -1.69E-46 |
| MS.gene058639 | MS.gene066718 | 0.846169 | 2.57E-59 | -1.69E-46 |
| MS.gene058852 | MS.gene066718 | 0.814297 | 1.66E-51 | -1.69E-46 |
| MS.gene059007 | MS.gene066718 | 0.808414 | 3.13E-50 | -1.69E-46 |
| MS.gene059171 | MS.gene066718 | 0.811214 | 7.83E-51 | -1.69E-46 |
| MS.gene059439 | MS.gene066718 | 0.809655 | 1.70E-50 | -1.69E-46 |
| MS.gene05978 | MS.gene066718 | 0.814361 | 1.60E-51 | -1.69E-46 |
| MS.gene060933 | MS.gene066718 | 0.831317 | 1.79E-55 | -1.69E-46 |
| MS.gene060935 | MS.gene066718 | 0.820167 | 7.91E-53 | -1.69E-46 |
| MS.gene060937 | MS.gene066718 | 0.82416 | 9.38E-54 | -1.69E-46 |
| MS.gene061006 | MS.gene066718 | 0.8068 | 6.90E-50 | -1.69E-46 |
| MS.gene061100 | MS.gene066718 | 0.826057 | 3.34E-54 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene066718.t1 | MTR_4g091290 | 98.052 | 154 | 3 | 0 | 1 | 154 | 20 | 173 | 2.13e-106 | 315 |
| MS.gene066718.t1 | MTR_8g098695 | 84.416 | 154 | 24 | 0 | 1 | 154 | 1 | 154 | 1.03e-90 | 275 |
| MS.gene066718.t1 | MTR_1g088950 | 56.954 | 151 | 65 | 0 | 4 | 154 | 11 | 161 | 3.60e-62 | 201 |
| MS.gene066718.t1 | MTR_7g083610 | 61.486 | 148 | 57 | 0 | 7 | 154 | 4 | 151 | 1.15e-60 | 196 |
| MS.gene066718.t1 | MTR_3g100860 | 54.194 | 155 | 67 | 1 | 4 | 154 | 14 | 168 | 6.11e-52 | 174 |
| MS.gene066718.t1 | MTR_5g065490 | 52.866 | 157 | 70 | 1 | 2 | 154 | 42 | 198 | 5.51e-50 | 169 |
| MS.gene066718.t1 | MTR_5g081870 | 40.816 | 147 | 86 | 1 | 9 | 154 | 13 | 159 | 1.81e-33 | 124 |
| MS.gene066718.t1 | MTR_3g070140 | 38.776 | 147 | 89 | 1 | 9 | 154 | 17 | 163 | 4.92e-31 | 117 |
| MS.gene066718.t1 | MTR_5g086490 | 46.032 | 126 | 53 | 1 | 7 | 132 | 4 | 114 | 6.26e-31 | 108 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene066718.t1 | AT1G12820 | 61.486 | 148 | 57 | 0 | 7 | 154 | 4 | 151 | 2.62e-62 | 201 |
| MS.gene066718.t1 | AT3G62980 | 61.486 | 148 | 57 | 0 | 7 | 154 | 9 | 156 | 5.24e-62 | 200 |
| MS.gene066718.t1 | AT3G26810 | 61.486 | 148 | 57 | 0 | 7 | 154 | 4 | 151 | 3.12e-61 | 198 |
| MS.gene066718.t1 | AT4G03190 | 57.432 | 148 | 63 | 0 | 7 | 154 | 5 | 152 | 4.67e-56 | 184 |
| MS.gene066718.t1 | AT4G24390 | 54.110 | 146 | 67 | 0 | 9 | 154 | 56 | 201 | 1.14e-50 | 171 |
| MS.gene066718.t1 | AT4G24390 | 54.110 | 146 | 67 | 0 | 9 | 154 | 56 | 201 | 1.14e-50 | 171 |
| MS.gene066718.t1 | AT5G49980 | 52.532 | 158 | 71 | 1 | 1 | 154 | 44 | 201 | 2.51e-50 | 170 |
| MS.gene066718.t1 | AT2G39940 | 40.816 | 147 | 86 | 1 | 9 | 154 | 18 | 164 | 2.19e-36 | 132 |
Find 25 sgRNAs with CRISPR-Local
Find 32 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TCAACTTGGTTCCTGCAAAT+TGG | 0.172792 | 4.2:-20966253 | MS.gene066718:CDS |
| AGAGAAAGAGCTTTGAAATT+AGG | 0.185626 | 4.2:+20966110 | None:intergenic |
| CAACTTGGTTCCTGCAAATT+GGG | 0.244113 | 4.2:-20966252 | MS.gene066718:CDS |
| AGTGTAACACTTCTTATGTT+TGG | 0.320668 | 4.2:+20966302 | None:intergenic |
| GGCTGTTAGTGATGAAAGTT+TGG | 0.351341 | 4.2:-20966153 | MS.gene066718:CDS |
| AGACAGAAGCTCAGTTTCAT+TGG | 0.392214 | 4.2:-20966423 | MS.gene066718:CDS |
| ACACGTTCCAACACTTCATC+TGG | 0.420875 | 4.2:+20966470 | None:intergenic |
| ATATCAGCACCCCAATTTGC+AGG | 0.422242 | 4.2:+20966242 | None:intergenic |
| GAAGTGTTGGAACGTGTTCT+TGG | 0.425811 | 4.2:-20966464 | MS.gene066718:CDS |
| AGCCTTAACTCTTCAAGAAA+AGG | 0.433803 | 4.2:+20966185 | None:intergenic |
| TGGATTTAGCACTGATGGCT+TGG | 0.443514 | 4.2:-20966075 | MS.gene066718:CDS |
| AGAGTTAAGGCTTAAGAGAA+TGG | 0.457818 | 4.2:-20966174 | MS.gene066718:CDS |
| TTTCATTGGTATGCAAAGAA+TGG | 0.457884 | 4.2:-20966409 | MS.gene066718:CDS |
| GGGGTGCTGATATTCATTCA+TGG | 0.461105 | 4.2:-20966232 | MS.gene066718:CDS |
| TGCTGATATTCATTCATGGT+TGG | 0.476550 | 4.2:-20966228 | MS.gene066718:CDS |
| TTTGAAATTAGGAAAAGAAA+AGG | 0.478989 | 4.2:+20966121 | None:intergenic |
| AAGTGTTGGAACGTGTTCTT+GGG | 0.483900 | 4.2:-20966463 | MS.gene066718:CDS |
| AATCTACGTGTCAAGATCTC+AGG | 0.511329 | 4.2:+20966326 | None:intergenic |
| AACTTGGTTCCTGCAAATTG+GGG | 0.521996 | 4.2:-20966251 | MS.gene066718:CDS |
| TGTGATGGATTTAGCACTGA+TGG | 0.530648 | 4.2:-20966080 | MS.gene066718:CDS |
| ATAAGAAGTGTTACACTGAA+AGG | 0.544660 | 4.2:-20966296 | MS.gene066718:CDS |
| AATGGTACAACGCTGAAAGA+TGG | 0.557820 | 4.2:-20966391 | MS.gene066718:CDS |
| GTTGGAACGTGTTCTTGGGA+TGG | 0.625125 | 4.2:-20966459 | MS.gene066718:CDS |
| ACAACGCTGAAAGATGGTCA+AGG | 0.670965 | 4.2:-20966385 | MS.gene066718:CDS |
| ACGCTGAAAGATGGTCAAGG+AGG | 0.726925 | 4.2:-20966382 | MS.gene066718:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | TTTGAAATTAGGAAAAGAAA+AGG | + | chr4.2:20966422-20966441 | None:intergenic | 20.0% |
| AAGTTGAAATCAGAAAAACG+AGG | + | chr4.2:20966274-20966293 | None:intergenic | 30.0% | |
| AGTGTAACACTTCTTATGTT+TGG | + | chr4.2:20966241-20966260 | None:intergenic | 30.0% | |
| TTTCATTGGTATGCAAAGAA+TGG | - | chr4.2:20966131-20966150 | MS.gene066718:CDS | 30.0% | |
| ! | ATCCTTTTCTTGAAGAGTTA+AGG | - | chr4.2:20966353-20966372 | MS.gene066718:CDS | 30.0% |
| !! | AGAGAAAGAGCTTTGAAATT+AGG | + | chr4.2:20966433-20966452 | None:intergenic | 30.0% |
| !! | ATAAGAAGTGTTACACTGAA+AGG | - | chr4.2:20966244-20966263 | MS.gene066718:CDS | 30.0% |
| !! | TCGTTTTTCTGATTTCAACT+TGG | - | chr4.2:20966273-20966292 | MS.gene066718:CDS | 30.0% |
| !! | TGATGAAAGTTTGGAGTTTT+TGG | - | chr4.2:20966396-20966415 | MS.gene066718:CDS | 30.0% |
| AGAGTTAAGGCTTAAGAGAA+TGG | - | chr4.2:20966366-20966385 | MS.gene066718:CDS | 35.0% | |
| AGCCTTAACTCTTCAAGAAA+AGG | + | chr4.2:20966358-20966377 | None:intergenic | 35.0% | |
| TGCTGATATTCATTCATGGT+TGG | - | chr4.2:20966312-20966331 | MS.gene066718:CDS | 35.0% | |
| ! | AGATTTTCCAGATGAAGTGT+TGG | - | chr4.2:20966063-20966082 | MS.gene066718:CDS | 35.0% |
| ! | TCAAGGAGGAATGTTTTCAT+AGG | - | chr4.2:20966172-20966191 | MS.gene066718:CDS | 35.0% |
| !!! | CTTTCTCTTTTGAGTTGTGA+TGG | - | chr4.2:20966445-20966464 | MS.gene066718:CDS | 35.0% |
| AACTTGGTTCCTGCAAATTG+GGG | - | chr4.2:20966289-20966308 | MS.gene066718:CDS | 40.0% | |
| AATCTACGTGTCAAGATCTC+AGG | + | chr4.2:20966217-20966236 | None:intergenic | 40.0% | |
| AATGGTACAACGCTGAAAGA+TGG | - | chr4.2:20966149-20966168 | MS.gene066718:CDS | 40.0% | |
| AGACAGAAGCTCAGTTTCAT+TGG | - | chr4.2:20966117-20966136 | MS.gene066718:CDS | 40.0% | |
| CAACTTGGTTCCTGCAAATT+GGG | - | chr4.2:20966288-20966307 | MS.gene066718:CDS | 40.0% | |
| GGCTGTTAGTGATGAAAGTT+TGG | - | chr4.2:20966387-20966406 | MS.gene066718:CDS | 40.0% | |
| TCAACTTGGTTCCTGCAAAT+TGG | - | chr4.2:20966287-20966306 | MS.gene066718:CDS | 40.0% | |
| !! | AAGTGTTGGAACGTGTTCTT+GGG | - | chr4.2:20966077-20966096 | MS.gene066718:CDS | 40.0% |
| !! | TGTGATGGATTTAGCACTGA+TGG | - | chr4.2:20966460-20966479 | MS.gene066718:CDS | 40.0% |
| ACAACGCTGAAAGATGGTCA+AGG | - | chr4.2:20966155-20966174 | MS.gene066718:CDS | 45.0% | |
| ACACGTTCCAACACTTCATC+TGG | + | chr4.2:20966073-20966092 | None:intergenic | 45.0% | |
| ATATCAGCACCCCAATTTGC+AGG | + | chr4.2:20966301-20966320 | None:intergenic | 45.0% | |
| !! | GAAGTGTTGGAACGTGTTCT+TGG | - | chr4.2:20966076-20966095 | MS.gene066718:CDS | 45.0% |
| !! | GGGGTGCTGATATTCATTCA+TGG | - | chr4.2:20966308-20966327 | MS.gene066718:CDS | 45.0% |
| !! | TGGATTTAGCACTGATGGCT+TGG | - | chr4.2:20966465-20966484 | MS.gene066718:CDS | 45.0% |
| ACGCTGAAAGATGGTCAAGG+AGG | - | chr4.2:20966158-20966177 | MS.gene066718:CDS | 50.0% | |
| GTTGGAACGTGTTCTTGGGA+TGG | - | chr4.2:20966081-20966100 | MS.gene066718:CDS | 50.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr4.2 | gene | 20966049 | 20966513 | 20966049 | ID=MS.gene066718 |
| chr4.2 | mRNA | 20966049 | 20966513 | 20966049 | ID=MS.gene066718.t1;Parent=MS.gene066718 |
| chr4.2 | exon | 20966049 | 20966513 | 20966049 | ID=MS.gene066718.t1.exon1;Parent=MS.gene066718.t1 |
| chr4.2 | CDS | 20966049 | 20966513 | 20966049 | ID=cds.MS.gene066718.t1;Parent=MS.gene066718.t1 |
| Gene Sequence |
| Protein sequence |
