AlfalfaGEDB | MS.gene066728

M. sativa cultivar XinJiangDaYe / MS.gene066728

KEGG: -
GO: GO:0003674 GO:0003824 GO:0016491
PFAM: PF13561.9

Nr:

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene066728.t1	XP_003626301.1	96	252	10	0	1	252	42	293	4.90E-133	483.8

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Swissprot:

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene066728.t1	Q9FZ42	78.6	252	54	0	1	252	37	288	1.1e-114	414.1

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Trembl:

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene066728.t1	Q2HTL8	96.0	252	10	0	1	252	42	293	3.5e-133	483.8

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TFs/TRs:

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Gene ID	Type	Classification
No data available in table

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Protein Kinases:

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Gene ID	Type	Classification
No data available in table

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Network:

Co-expression Network:

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Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene050862	MS.gene066728	0.966943	1.33E-126	-1.69E-46

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PPI:

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Gene1	Gene2	Type
MS.gene019786	MS.gene066728	PPI
MS.gene021164	MS.gene066728	PPI
MS.gene050431	MS.gene066728	PPI
MS.gene050432	MS.gene066728	PPI
MS.gene066728	MS.gene072664	PPI
MS.gene066728	MS.gene07097	PPI
MS.gene066728	MS.gene31507	PPI
MS.gene066728	MS.gene043387	PPI
MS.gene066728	MS.gene30551	PPI
MS.gene066728	MS.gene03319	PPI

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene066728.t1	MTR_7g113650	98.810	252	3	0	1	252	42	293	0.0	518
MS.gene066728.t1	MTR_1g082760	88.446	251	29	0	1	251	42	292	3.51e-170	471
MS.gene066728.t1	MTR_4g131740	70.751	253	73	1	1	252	42	294	5.17e-131	372
MS.gene066728.t1	MTR_6g023590	33.462	260	146	9	1	247	15	260	4.70e-32	118
MS.gene066728.t1	MTR_6g023950	34.118	255	151	7	1	247	15	260	5.44e-31	115
MS.gene066728.t1	MTR_6g023610	32.950	261	150	8	1	249	15	262	1.96e-30	114
MS.gene066728.t1	MTR_6g023990	33.205	259	148	8	1	247	16	261	9.28e-30	112
MS.gene066728.t1	MTR_6g023600	34.615	260	143	9	1	247	15	260	1.74e-29	112
MS.gene066728.t1	MTR_3g085740	30.651	261	149	7	3	250	79	320	5.41e-29	112
MS.gene066728.t1	MTR_7g063270	33.858	254	152	8	1	247	18	262	2.57e-28	108

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene066728.t1	AT1G54870	80.159	252	50	0	1	252	84	335	1.85e-157	441
MS.gene066728.t1	AT1G54870	80.159	252	50	0	1	252	86	337	2.56e-157	440
MS.gene066728.t1	AT3G05260	72.619	252	68	1	1	252	39	289	5.46e-140	394
MS.gene066728.t1	AT4G05530	30.000	250	161	6	1	247	11	249	9.52e-25	99.4
MS.gene066728.t1	AT5G06060	33.333	255	157	8	1	251	11	256	4.99e-23	94.7
MS.gene066728.t1	AT3G51680	28.889	270	159	9	1	247	34	293	1.21e-21	92.0
MS.gene066728.t1	AT1G24360	27.778	252	168	5	3	250	78	319	1.43e-21	92.0
MS.gene066728.t1	AT2G29350	31.518	257	158	7	1	251	17	261	3.59e-21	90.1
MS.gene066728.t1	AT3G42960	29.389	262	156	5	2	247	11	259	9.02e-21	89.0
MS.gene066728.t1	AT3G04000	28.077	260	174	7	1	250	16	272	1.10e-20	88.6

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Find 62 sgRNAs with CRISPR-Local

Find 82 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAGATAGCAGTGGTGACTGG+AGG	0.668759	7.1:-2838983	MS.gene066728:CDS
AAGGAGGGAGCAACCTCAAC+AGG	0.426792	7.1:+2838254	None:intergenic
AATACCAGCTTCCTTCAATG+AGG	0.587076	7.1:-2838327	MS.gene066728:CDS
AATGGAGTCCAAATAGGTCC+AGG	0.558031	7.1:+2838350	None:intergenic
ACATCAGTGAATGCATACAA+AGG	0.633175	7.1:-2838482	MS.gene066728:CDS
ACTGGAGGTGACTCTGGAAT+AGG	0.468261	7.1:-2838968	MS.gene066728:CDS
AGAGTGAATGGGGTTGCACC+TGG	0.511342	7.1:-2838368	MS.gene066728:CDS
AGCAAAGGTATAAGAGTGAA+TGG	0.447089	7.1:-2838380	MS.gene066728:CDS
AGGTCAGCTGGTATAGCCAT+TGG	0.542312	7.1:+2838812	None:intergenic
ATGGCTATACCAGCTGACCT+TGG	0.565262	7.1:-2838809	MS.gene066728:CDS

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
ATTAAGAGTGGTTTATTACC+TGG	+	chr7.1:2838455-2838474	None:intergenic	30.0%
ACATCAGTGAATGCATACAA+AGG	-	chr7.1:2838583-2838602	MS.gene066728:intron	35.0%
ATTGACTACACGTCTACTAA+GGG	-	chr7.1:2838619-2838638	MS.gene066728:intron	35.0%
CACATATGTGAAAATCACAG+TGG	+	chr7.1:2838158-2838177	None:intergenic	35.0%
CATCAGTGAATGCATACAAA+GGG	-	chr7.1:2838584-2838603	MS.gene066728:intron	35.0%
GATGAGATCATCAATGCTTA+TGG	-	chr7.1:2838307-2838326	MS.gene066728:CDS	35.0%
GGTATTAATGGAGTCCAAAT+AGG	+	chr7.1:2838724-2838743	None:intergenic	35.0%
TGATGAGAATTGCAAAAGAG+TGG	-	chr7.1:2838282-2838301	MS.gene066728:CDS	35.0%
TGCAAATGCTAAAGATCCAA+TGG	-	chr7.1:2838237-2838256	MS.gene066728:CDS	35.0%
TGCAATTCTCATCAAAACCA+AGG	+	chr7.1:2838276-2838295	None:intergenic	35.0%

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Chromosome	Type	Strat	End	Strand	Name
chr7.1	gene	2838079	2839008	2838079	ID=MS.gene066728
chr7.1	mRNA	2838079	2839008	2838079	ID=MS.gene066728.t1;Parent=MS.gene066728
chr7.1	exon	2838632	2839008	2838632	ID=MS.gene066728.t1.exon1;Parent=MS.gene066728.t1
chr7.1	CDS	2838632	2839008	2838632	ID=cds.MS.gene066728.t1;Parent=MS.gene066728.t1
chr7.1	exon	2838192	2838550	2838192	ID=MS.gene066728.t1.exon2;Parent=MS.gene066728.t1
chr7.1	CDS	2838192	2838550	2838192	ID=cds.MS.gene066728.t1;Parent=MS.gene066728.t1
chr7.1	exon	2838079	2838101	2838079	ID=MS.gene066728.t1.exon3;Parent=MS.gene066728.t1
chr7.1	CDS	2838079	2838101	2838079	ID=cds.MS.gene066728.t1;Parent=MS.gene066728.t1

Gene Sequence

>MS.gene066728

GGAAAGATAGCAGTGGTGACTGGAGGTGACTCTGGAATAGGACGTGCAGTATGCAACTTGTTTGCATTAGAAGGTGCCACTGTGATTTTCACATATGTGAAGGGTCATGAAGACAAGGACGCAAGGGACACACTTGATATGTTAAAAATGGCTAAGGCTGCAAATGCTAAAGATCCAATGGCTATACCAGCTGACCTTGGTTTTGATGAGAATTGCAAAAGAGTGGTTGATGAGATCATCAATGCTTATGGCAGAATTGATATTCTTGTCAACAATGCAGCTGAGCAGTATGAGTGTGGTTCAGTTGAGGAGATTGATGAACCAAGACTCGAGAGGGTGTTTAGAACTAACATCTTCTCCTATTTCTTCATGACCAGGTAATAAACCACTCTTAATTAAATTAGATAAATCGGATGTTATTTCATGAATTTGGGGTTGACTAACTATTTGTGTAATAGGCATGCCTTGAAGCATATGAAGGAAGGAAGCAGCATTATTAACACAACATCAGTGAATGCATACAAAGGGAACTCGACATTGATTGACTACACGTCTACTAAGGGTGCCATTGTGGCTTTTACAAGGGCACTTTCACTTCAGCTTGTGAGCAAAGGTATAAGAGTGAATGGGGTTGCACCTGGACCTATTTGGACTCCATTAATACCAGCTTCCTTCAATGAGGAAAAGACTGCTCAGTTTGGTAGTGATGTGCCAATGAAGAGGGCTGGACAGCCTGTTGAGGTTGCTCCCTCCTTTGTGTTTCTTGCTTCCAACCAATGCTCCTCTTACATAACTGGCCAAGTCCTCCACCCTAACGGTGATATACTTATAAATGCTTCTATGTTATATATTTGTTCACACATTCTATGGTCTTTCTAACACATTCTTTTCATTTTTTATTTTTCAGGAGGAACCGTTGTGAATGCATAG

Protein sequence

>MS.gene066728.t1

GKIAVVTGGDSGIGRAVCNLFALEGATVIFTYVKGHEDKDARDTLDMLKMAKAANAKDPMAIPADLGFDENCKRVVDEIINAYGRIDILVNNAAEQYECGSVEEIDEPRLERVFRTNIFSYFFMTRHALKHMKEGSSIINTTSVNAYKGNSTLIDYTSTKGAIVAFTRALSLQLVSKGIRVNGVAPGPIWTPLIPASFNEEKTAQFGSDVPMKRAGQPVEVAPSFVFLASNQCSSYITGQVLHPNGGTVVNA