AlfalfaGEDB | MS.gene068637

M. sativa cultivar XinJiangDaYe / MS.gene068637

KEGG: K05752
GO: -
PFAM: -

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene068637.t1	XP_003517200.1	100	85	0	0	1	85	1	85	2.20E-37	164.5

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene068637.t1	Q94JY4	90.6	85	8	0	1	85	1	85	1.6e-36	152.9

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene068637.t1	A0A0B2PBB9	100.0	85	0	0	1	85	1	85	1.6e-37	164.5

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene049255	MS.gene068637	0.813193	2.90E-51	-1.69E-46
MS.gene049341	MS.gene068637	0.813696	2.25E-51	-1.69E-46
MS.gene049424	MS.gene068637	0.810979	8.80E-51	-1.69E-46
MS.gene049756	MS.gene068637	0.802424	5.65E-49	-1.69E-46
MS.gene049821	MS.gene068637	0.809626	1.72E-50	-1.69E-46
MS.gene050020	MS.gene068637	0.80317	3.96E-49	-1.69E-46
MS.gene050277	MS.gene068637	0.802918	4.47E-49	-1.69E-46
MS.gene050303	MS.gene068637	0.816	6.93E-52	-1.69E-46
MS.gene050750	MS.gene068637	0.804754	1.86E-49	-1.69E-46
MS.gene050807	MS.gene068637	0.832021	1.20E-55	-1.69E-46
MS.gene050815	MS.gene068637	0.817481	3.22E-52	-1.69E-46
MS.gene050953	MS.gene068637	0.807709	4.43E-50	-1.69E-46
MS.gene051241	MS.gene068637	0.804724	1.88E-49	-1.69E-46
MS.gene051243	MS.gene068637	0.830032	3.69E-55	-1.69E-46
MS.gene051328	MS.gene068637	0.827609	1.42E-54	-1.69E-46
MS.gene05134	MS.gene068637	0.807761	4.32E-50	-1.69E-46
MS.gene052133	MS.gene068637	0.801821	7.51E-49	-1.69E-46
MS.gene052176	MS.gene068637	0.807453	5.02E-50	-1.69E-46
MS.gene052286	MS.gene068637	0.803776	2.97E-49	-1.69E-46
MS.gene052302	MS.gene068637	0.825687	4.09E-54	-1.69E-46
MS.gene052376	MS.gene068637	0.826435	2.72E-54	-1.69E-46
MS.gene052519	MS.gene068637	0.810999	8.72E-51	-1.69E-46
MS.gene054657	MS.gene068637	0.804622	1.98E-49	-1.69E-46
MS.gene05510	MS.gene068637	0.801742	7.80E-49	-1.69E-46
MS.gene055778	MS.gene068637	0.80181	7.55E-49	-1.69E-46
MS.gene055808	MS.gene068637	0.801089	1.06E-48	-1.69E-46
MS.gene055818	MS.gene068637	0.811634	6.35E-51	-1.69E-46
MS.gene056498	MS.gene068637	0.805023	1.63E-49	-1.69E-46
MS.gene056631	MS.gene068637	0.812874	3.40E-51	-1.69E-46
MS.gene057060	MS.gene068637	0.845684	3.48E-59	-1.69E-46
MS.gene057079	MS.gene068637	0.812354	4.42E-51	-1.69E-46
MS.gene057314	MS.gene068637	0.811679	6.21E-51	-1.69E-46
MS.gene057693	MS.gene068637	0.82404	1.00E-53	-1.69E-46
MS.gene057705	MS.gene068637	0.834328	3.19E-56	-1.69E-46
MS.gene058084	MS.gene068637	0.806351	8.58E-50	-1.69E-46
MS.gene058906	MS.gene068637	0.808822	2.56E-50	-1.69E-46
MS.gene059317	MS.gene068637	0.819298	1.25E-52	-1.69E-46
MS.gene05954	MS.gene068637	0.844222	8.64E-59	-1.69E-46
MS.gene059569	MS.gene068637	0.804544	2.05E-49	-1.69E-46
MS.gene059842	MS.gene068637	0.802444	5.59E-49	-1.69E-46
MS.gene060322	MS.gene068637	0.821389	4.14E-53	-1.69E-46
MS.gene060429	MS.gene068637	0.832487	9.19E-56	-1.69E-46
MS.gene060634	MS.gene068637	-0.813681	2.26E-51	-1.69E-46
MS.gene060803	MS.gene068637	0.808509	2.99E-50	-1.69E-46
MS.gene060917	MS.gene068637	0.804118	2.52E-49	-1.69E-46
MS.gene061040	MS.gene068637	-0.807148	5.82E-50	-1.69E-46

PPI:

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene068637.t1	MTR_5g030935	100.000	85	0	0	1	85	1	85	2.67e-57	171

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene068637.t1	AT2G22640	90.588	85	8	0	1	85	1	85	6.83e-53	159

Find 12 sgRNAs with CRISPR-Local

Find 16 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
TGATGTATTGGAACGTCGTT+TGG	0.348132	5.1:-21217104	MS.gene068637:CDS
GAAGAAATTCAGTACCGAAT+TGG	0.368507	5.1:+21217260	None:intergenic
GAATCGCTGTTCAAGCTGAT+TGG	0.386107	5.1:-21217345	MS.gene068637:CDS
TTCGATTTCCTTGTCCAATT+CGG	0.396293	5.1:-21217274	MS.gene068637:intron
GAATGAGAAGCTTGATGTAT+TGG	0.424872	5.1:-21217116	MS.gene068637:CDS
AATCGCTGTTCAAGCTGATT+GGG	0.456401	5.1:-21217344	MS.gene068637:CDS
GAATTACTTGAGGTTCAAGT+TGG	0.513164	5.1:-21217082	MS.gene068637:CDS
ATTCCATCAATGGCACGTGC+AGG	0.538482	5.1:-21217394	None:intergenic
ACGTCGTTTGGAATTACTTG+AGG	0.559248	5.1:-21217092	MS.gene068637:CDS
CCATCAATGGCACGTGCAGG+AGG	0.597647	5.1:-21217391	None:intergenic
ATTCAGTACCGAATTGGACA+AGG	0.614288	5.1:+21217266	None:intergenic
ATCACAAACGCAGTTAACGT+TGG	0.624596	5.1:-21217367	MS.gene068637:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!!	AAATCTTATATCTTTTTTGA+TGG	-	chr5.1:21217235-21217254	MS.gene068637:intron	15.0%
!!!	AATCTTATATCTTTTTTGAT+GGG	-	chr5.1:21217236-21217255	MS.gene068637:intron	15.0%
!!	ATATAAGATTTCAACTAACA+AGG	+	chr5.1:21217226-21217245	None:intergenic	20.0%
!!!	TGTTATTTTTGTTATTTCAG+AGG	-	chr5.1:21217263-21217282	MS.gene068637:intron	20.0%
	ATTTCAACTAACAAGGCAAA+AGG	+	chr5.1:21217219-21217238	None:intergenic	30.0%
	TTTCAACTAACAAGGCAAAA+GGG	+	chr5.1:21217218-21217237	None:intergenic	30.0%
	GAATGAGAAGCTTGATGTAT+TGG	-	chr5.1:21217314-21217333	MS.gene068637:CDS	35.0%
	GAATTACTTGAGGTTCAAGT+TGG	-	chr5.1:21217348-21217367	MS.gene068637:CDS	35.0%
	TTCGATTTCCTTGTCCAATT+CGG	-	chr5.1:21217156-21217175	MS.gene068637:intron	35.0%
!	GAAGAAATTCAGTACCGAAT+TGG	+	chr5.1:21217173-21217192	None:intergenic	35.0%
	AATCGCTGTTCAAGCTGATT+GGG	-	chr5.1:21217086-21217105	MS.gene068637:CDS	40.0%
	ATCACAAACGCAGTTAACGT+TGG	-	chr5.1:21217063-21217082	MS.gene068637:CDS	40.0%
	TGATGTATTGGAACGTCGTT+TGG	-	chr5.1:21217326-21217345	MS.gene068637:CDS	40.0%
!	ACGTCGTTTGGAATTACTTG+AGG	-	chr5.1:21217338-21217357	MS.gene068637:CDS	40.0%
!	ATTCAGTACCGAATTGGACA+AGG	+	chr5.1:21217167-21217186	None:intergenic	40.0%
	GAATCGCTGTTCAAGCTGAT+TGG	-	chr5.1:21217085-21217104	MS.gene068637:CDS	45.0%

Chromosome	Type	Strat	End	Strand	Name
chr5.1	gene	21217045	21217407	21217045	ID=MS.gene068637
chr5.1	mRNA	21217045	21217407	21217045	ID=MS.gene068637.t1;Parent=MS.gene068637
chr5.1	exon	21217275	21217407	21217275	ID=MS.gene068637.t1.exon1;Parent=MS.gene068637.t1
chr5.1	CDS	21217275	21217407	21217275	ID=cds.MS.gene068637.t1;Parent=MS.gene068637.t1
chr5.1	exon	21217045	21217169	21217045	ID=MS.gene068637.t1.exon2;Parent=MS.gene068637.t1
chr5.1	CDS	21217045	21217169	21217045	ID=cds.MS.gene068637.t1;Parent=MS.gene068637.t1

Gene Sequence	>MS.gene068637 ATGGCACGTGCAGGAGGAATCACAAACGCAGTTAACGTTGGAATCGCTGTTCAAGCTGATTGGGAAAATCGTGAATTCATCTCTCACATTTCTCTCAATGTTCGTCGTCTCTTCGATTTCCTTGTCCAATTCGGTACTGAATTTCTTCTCAATTTTCTGTTTTTCTTTGACCCTTTTGCCTTGTTAGTTGAAATCTTATATCTTTTTTGATGGGTTGTTGTTATTTTTGTTATTTCAGAGGCTACAACAAAGAGTAAATTAGCTTCATTGAATGAGAAGCTTGATGTATTGGAACGTCGTTTGGAATTACTTGAGGTTCAAGTTGGCAACGCTTCTGCAAATCCTTCTCTTTTTGCAACTTGA
Protein sequence	>MS.gene068637.t1 MARAGGITNAVNVGIAVQADWENREFISHISLNVRRLFDFLVQFEATTKSKLASLNEKLDVLERRLELLEVQVGNASANPSLFAT