AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene071152


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene071152.t1 MTR_7g056337 100.000 153 0 0 1 153 63 215 2.29e-112 317
MS.gene071152.t1 MTR_8g087430 71.242 153 44 0 1 153 63 215 6.33e-79 233
MS.gene071152.t1 MTR_4g075550 34.839 155 86 5 12 153 117 269 2.14e-22 90.1
MS.gene071152.t1 MTR_3g113920 34.783 161 90 5 6 153 111 269 9.68e-22 88.6
MS.gene071152.t1 MTR_8g012615 33.129 163 94 3 1 150 40 200 6.22e-19 79.7
MS.gene071152.t1 MTR_8g012595 31.579 152 88 3 12 149 118 267 3.51e-16 73.6
MS.gene071152.t1 MTR_8g012605 36.036 111 64 3 1 107 107 214 2.91e-14 68.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene071152.t1 AT3G25040 85.621 153 22 0 1 153 63 215 9.09e-98 281
MS.gene071152.t1 AT1G29330 70.588 153 45 0 1 153 1 153 1.15e-79 233
MS.gene071152.t1 AT1G29330 70.588 153 45 0 1 153 63 215 1.34e-79 234
MS.gene071152.t1 AT1G75760 34.783 161 90 5 6 153 111 269 2.04e-22 90.1
MS.gene071152.t1 AT1G19970 35.484 155 85 5 12 153 117 269 5.52e-22 89.0
MS.gene071152.t1 AT4G38790 33.735 166 95 5 1 153 107 270 2.28e-21 87.4
MS.gene071152.t1 AT1G75760 33.784 148 84 4 6 141 111 256 1.07e-20 85.5
MS.gene071152.t1 AT3G25160 33.333 156 87 5 6 147 113 265 9.16e-20 83.2
MS.gene071152.t1 AT2G21190 31.928 166 98 5 1 153 103 266 1.45e-19 82.8
MS.gene071152.t1 AT1G75760 35.238 105 63 2 6 107 111 213 7.16e-17 74.7
MS.gene071152.t1 AT1G19970 36.364 99 58 2 12 107 117 213 4.28e-16 72.8

Find 34 sgRNAs with CRISPR-Local

Find 91 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CAATGAGAAATTCACATTTA+AGG 0.174751 7.3:-48890375 MS.gene071152:CDS
TGTTATGAAGTTGATATTCT+TGG 0.275463 7.3:-48890525 None:intergenic
GGACAATATGTCTTTCTTCT+TGG 0.280895 7.3:-48890121 MS.gene071152:CDS
AGTATTGCCCTGCTTATTGT+TGG 0.324545 7.3:-48890408 MS.gene071152:CDS
AGAAACATTGACAATTTAAC+AGG 0.337581 7.3:-48890142 MS.gene071152:CDS
GACAATATGTCTTTCTTCTT+GGG 0.345288 7.3:-48890120 MS.gene071152:intron
GAGCACTATACATTCTCAAC+TGG 0.354642 7.3:-48889382 MS.gene071152:CDS
TGAATTTCTCATTGATAAGT+AGG 0.405613 7.3:+48890384 None:intergenic
CAGTACCAGTTGAGGAAGTA+TGG 0.407791 7.3:+48890177 None:intergenic
TGGCAACAGCTTCCAAATAC+AGG 0.408291 7.3:+48890197 None:intergenic
GTTATGAAGTTGATATTCTT+GGG 0.424245 7.3:-48890524 MS.gene071152:CDS
TGTTGCCATACTTCCTCAAC+TGG 0.450840 7.3:-48890182 MS.gene071152:CDS
CCGAGCCCCACTTTGTCCAT+TGG 0.453725 7.3:-48889343 MS.gene071152:CDS
ATGCTGGCAAATGAAGCTTC+TGG 0.490077 7.3:+48889019 None:intergenic
AAGTAGGGCCAACAATAAGC+AGG 0.512666 7.3:+48890400 None:intergenic
TAGATCATATGACAAAGATC+AGG 0.514146 7.3:-48890453 MS.gene071152:CDS
CCAATGGACAAAGTGGGGCT+CGG 0.529481 7.3:+48889343 None:intergenic
TACTTATCCAATGGACAAAG+TGG 0.529817 7.3:+48889336 None:intergenic
TATGGAGAATTACACTATGC+TGG 0.546192 7.3:+48889003 None:intergenic
CTTTGTAGCAGTACCAGTTG+AGG 0.551696 7.3:+48890169 None:intergenic
TTTCTATTGTATGGTACATG+AGG 0.554169 7.3:-48890493 MS.gene071152:CDS
TCTTCTACTACTATTTCCAA+AGG 0.567268 7.3:-48889142 MS.gene071152:intron
TGAGAAATTCACATTTAAGG+AGG 0.576216 7.3:-48890372 MS.gene071152:intron
GAATTTCTCATTGATAAGTA+GGG 0.579233 7.3:+48890385 None:intergenic
ACTTATCCAATGGACAAAGT+GGG 0.585109 7.3:+48889337 None:intergenic
TACTAGAAAATAGTGACGGA+AGG 0.599670 7.3:+48890427 None:intergenic
ACAGAATTATACTTATCCAA+TGG 0.602023 7.3:+48889327 None:intergenic
GGCAACAGCTTCCAAATACA+GGG 0.635959 7.3:+48890198 None:intergenic
CTTATCCAATGGACAAAGTG+GGG 0.636328 7.3:+48889338 None:intergenic
TATACATGGAATAGGTGATG+TGG 0.652075 7.3:-48890228 MS.gene071152:intron
AGCACTATACATTCTCAACT+GGG 0.652774 7.3:-48889381 MS.gene071152:CDS
GCAATACTAGAAAATAGTGA+CGG 0.652982 7.3:+48890423 None:intergenic
ATGATCTACGAACAATCTTG+TGG 0.654874 7.3:+48890468 None:intergenic
AGTAGGGCCAACAATAAGCA+GGG 0.670399 7.3:+48890401 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATGCAAATATATAATGTTAA+AGG + chr7.3:48889455-48889474 None:intergenic 15.0%
!! CTATAATAAAAAATAAGCAA+TGG - chr7.3:48890022-48890041 MS.gene071152:intron 15.0%
!! GGATTATTAACATAAATAAA+TGG + chr7.3:48890444-48890463 None:intergenic 15.0%
!! CTAGATAAATTATAGCTAAT+GGG - chr7.3:48889775-48889794 MS.gene071152:intron 20.0%
!! TAAAAACATTCAAACATGTA+TGG + chr7.3:48889237-48889256 None:intergenic 20.0%
!! TAGATAAATTATAGCTAATG+GGG - chr7.3:48889776-48889795 MS.gene071152:intron 20.0%
!!! AGTTGAAAGTTTTTATTTGT+TGG - chr7.3:48889909-48889928 MS.gene071152:intron 20.0%
!!! TCTTGTTTAATTGTTATCAT+TGG - chr7.3:48890235-48890254 MS.gene071152:intron 20.0%
! AAAGCTAAAACAAGTTCAAA+TGG + chr7.3:48890070-48890089 None:intergenic 25.0%
! AAGCTAAAACAAGTTCAAAT+GGG + chr7.3:48890069-48890088 None:intergenic 25.0%
! AATTAGCAGCTATTATATTC+TGG - chr7.3:48889507-48889526 MS.gene071152:intron 25.0%
! ACAGAATTATACTTATCCAA+TGG + chr7.3:48890214-48890233 None:intergenic 25.0%
! ACTGTAATGTTTAACAATGA+TGG + chr7.3:48890289-48890308 None:intergenic 25.0%
! AGAAACATTGACAATTTAAC+AGG - chr7.3:48889396-48889415 MS.gene071152:intron 25.0%
! ATATATTTGCATGATTTCTG+TGG - chr7.3:48889463-48889482 MS.gene071152:intron 25.0%
! ATCATTGTTAAACATTACAG+TGG - chr7.3:48890288-48890307 MS.gene071152:intron 25.0%
! CAATGAGAAATTCACATTTA+AGG - chr7.3:48889163-48889182 MS.gene071152:CDS 25.0%
! CCATTAGCTATAATTTATCT+AGG + chr7.3:48889777-48889796 None:intergenic 25.0%
! CCTAGATAAATTATAGCTAA+TGG - chr7.3:48889774-48889793 MS.gene071152:intron 25.0%
! GAATTTCTCATTGATAAGTA+GGG + chr7.3:48889156-48889175 None:intergenic 25.0%
! TACTATTTCCAAAGGTAAAT+TGG - chr7.3:48890404-48890423 MS.gene071152:CDS 25.0%
! TATAATTTATCTAGGTGAGT+TGG + chr7.3:48889769-48889788 None:intergenic 25.0%
! TGAATTTCTCATTGATAAGT+AGG + chr7.3:48889157-48889176 None:intergenic 25.0%
! TTTCATGTAACATGTTGTAT+AGG - chr7.3:48889727-48889746 MS.gene071152:intron 25.0%
!!! TTGAACTTGTTTTAGCTTTT+AGG - chr7.3:48890071-48890090 MS.gene071152:intron 25.0%
AAACTGCTTATACATGGAAT+AGG - chr7.3:48889302-48889321 MS.gene071152:intron 30.0%
AACCTTAATAAGCAGAACTT+TGG + chr7.3:48889967-48889986 None:intergenic 30.0%
ATGGTAACTTTGTATATCAG+AGG - chr7.3:48890041-48890060 MS.gene071152:intron 30.0%
CAACTCAAGATCATAATAGA+AGG + chr7.3:48889894-48889913 None:intergenic 30.0%
GACAATATGTCTTTCTTCTT+GGG - chr7.3:48889418-48889437 MS.gene071152:intron 30.0%
GCAATACTAGAAAATAGTGA+CGG + chr7.3:48889118-48889137 None:intergenic 30.0%
TAGATCATATGACAAAGATC+AGG - chr7.3:48889085-48889104 MS.gene071152:intron 30.0%
TCTTCTACTACTATTTCCAA+AGG - chr7.3:48890396-48890415 MS.gene071152:CDS 30.0%
TGAAAATATTGCCTTGTAGT+TGG - chr7.3:48890488-48890507 MS.gene071152:CDS 30.0%
TGAGAAATTCACATTTAAGG+AGG - chr7.3:48889166-48889185 MS.gene071152:CDS 30.0%
TTTCTATTGTATGGTACATG+AGG - chr7.3:48889045-48889064 MS.gene071152:intron 30.0%
! ATTCCATGTATAAGCAGTTT+TGG + chr7.3:48889302-48889321 None:intergenic 30.0%
! GAAGCTCATTTTCTATTGTA+TGG - chr7.3:48889036-48889055 MS.gene071152:intron 30.0%
! TTCCATGTATAAGCAGTTTT+GGG + chr7.3:48889301-48889320 None:intergenic 30.0%
! TTTATTTGTTGGAACGAAAC+TGG - chr7.3:48889920-48889939 MS.gene071152:intron 30.0%
AATAAATGGACCTGACAAGT+AGG + chr7.3:48890430-48890449 None:intergenic 35.0%
ACTTATCCAATGGACAAAGT+GGG + chr7.3:48890204-48890223 None:intergenic 35.0%
AGCACTATACATTCTCAACT+GGG - chr7.3:48890157-48890176 MS.gene071152:CDS 35.0%
AGCCAAAGTTCTGCTTATTA+AGG - chr7.3:48889962-48889981 MS.gene071152:intron 35.0%
ATGATCTACGAACAATCTTG+TGG + chr7.3:48889073-48889092 None:intergenic 35.0%
GGACAATATGTCTTTCTTCT+TGG - chr7.3:48889417-48889436 MS.gene071152:intron 35.0%
GGCTTCAAGATCATAATAGA+AGG + chr7.3:48889946-48889965 None:intergenic 35.0%
TACTTATCCAATGGACAAAG+TGG + chr7.3:48890205-48890224 None:intergenic 35.0%
TATACATGGAATAGGTGATG+TGG - chr7.3:48889310-48889329 MS.gene071152:intron 35.0%
TTGAAACTGATCAATGAGAC+TGG - chr7.3:48889868-48889887 MS.gene071152:intron 35.0%
TTGGTTTATGCTCTTTAGAC+TGG - chr7.3:48890254-48890273 MS.gene071152:intron 35.0%
TTTCAATGAAGCCAACAAAC+AGG + chr7.3:48889854-48889873 None:intergenic 35.0%
! CATTACTTCTTTTGCAGCAT+GGG - chr7.3:48890337-48890356 MS.gene071152:intron 35.0%
! TACTAGAAAATAGTGACGGA+AGG + chr7.3:48889114-48889133 None:intergenic 35.0%
AATGGAAGGAAGTCAGAACA+AGG + chr7.3:48890321-48890340 None:intergenic 40.0%
AGACCAACCTTCCAGTTATT+TGG + chr7.3:48889558-48889577 None:intergenic 40.0%
AGTATTGCCCTGCTTATTGT+TGG - chr7.3:48889130-48889149 MS.gene071152:intron 40.0%
CAAGTAGGCCAATTTACCTT+TGG + chr7.3:48890415-48890434 None:intergenic 40.0%
CCATGCTGCAAAAGAAGTAA+TGG + chr7.3:48890339-48890358 None:intergenic 40.0%
CTGATCAACAGCCAAATAAC+TGG - chr7.3:48889544-48889563 MS.gene071152:intron 40.0%
CTTATCCAATGGACAAAGTG+GGG + chr7.3:48890203-48890222 None:intergenic 40.0%
CTTTCTTCTTGGGTAAGCTA+TGG - chr7.3:48889428-48889447 MS.gene071152:intron 40.0%
CTTTGTCTGTGAAATGCAGT+GGG - chr7.3:48890129-48890148 MS.gene071152:CDS 40.0%
GAGCACTATACATTCTCAAC+TGG - chr7.3:48890156-48890175 MS.gene071152:CDS 40.0%
GCTCAAAACTAAGACGAACA+AGG + chr7.3:48890465-48890484 None:intergenic 40.0%
GTCCCAAAACTGCTTATACA+TGG - chr7.3:48889296-48889315 MS.gene071152:intron 40.0%
TAGCTAATGGGGACAACAAA+AGG - chr7.3:48889787-48889806 MS.gene071152:intron 40.0%
TCAACAGCCAAATAACTGGA+AGG - chr7.3:48889548-48889567 MS.gene071152:intron 40.0%
TGGTTGTTCTTCCAACTACA+AGG + chr7.3:48890502-48890521 None:intergenic 40.0%
! CCATTACTTCTTTTGCAGCA+TGG - chr7.3:48890336-48890355 MS.gene071152:intron 40.0%
! GCTGCAAAAGAAGTAATGGA+AGG + chr7.3:48890335-48890354 None:intergenic 40.0%
! GTGAGTTGGATTGACAACTT+TGG + chr7.3:48889755-48889774 None:intergenic 40.0%
! GTGGACATTTTCCCTGTATT+TGG - chr7.3:48889329-48889348 MS.gene071152:intron 40.0%
AAGTAGGGCCAACAATAAGC+AGG + chr7.3:48889141-48889160 None:intergenic 45.0%
AGTAGGGCCAACAATAAGCA+GGG + chr7.3:48889140-48889159 None:intergenic 45.0%
ATGCTGGCAAATGAAGCTTC+TGG + chr7.3:48890522-48890541 None:intergenic 45.0%
CAGCCAAATAACTGGAAGGT+TGG - chr7.3:48889552-48889571 MS.gene071152:intron 45.0%
CAGTACCAGTTGAGGAAGTA+TGG + chr7.3:48889364-48889383 None:intergenic 45.0%
CTTTGTAGCAGTACCAGTTG+AGG + chr7.3:48889372-48889391 None:intergenic 45.0%
GCATAGAACGAGCTTAGTAG+TGG - chr7.3:48889596-48889615 MS.gene071152:intron 45.0%
GCTTTGTCTGTGAAATGCAG+TGG - chr7.3:48890128-48890147 MS.gene071152:CDS 45.0%
GGCAACAGCTTCCAAATACA+GGG + chr7.3:48889343-48889362 None:intergenic 45.0%
TGGCAACAGCTTCCAAATAC+AGG + chr7.3:48889344-48889363 None:intergenic 45.0%
TGTTGCCATACTTCCTCAAC+TGG - chr7.3:48889356-48889375 MS.gene071152:CDS 45.0%
! CTTTTGCAGCATGGGTTTCT+GGG - chr7.3:48890345-48890364 MS.gene071152:intron 45.0%
! GGTAAATTGGCCTACTTGTC+AGG - chr7.3:48890417-48890436 MS.gene071152:CDS 45.0%
! TCTTTTGCAGCATGGGTTTC+TGG - chr7.3:48890344-48890363 MS.gene071152:intron 45.0%
!! TTAGTTTGCAGTAGTGCTCG+TGG - chr7.3:48889823-48889842 MS.gene071152:intron 45.0%
TCGTGGACAAGCCTGTTTGT+TGG - chr7.3:48889840-48889859 MS.gene071152:intron 50.0%
CCAATGGACAAAGTGGGGCT+CGG + chr7.3:48890198-48890217 None:intergenic 55.0%
CCGAGCCCCACTTTGTCCAT+TGG - chr7.3:48890195-48890214 MS.gene071152:CDS 60.0%


Chromosome Type Strat End Strand Name
chr7.3 gene 48889017 48890543 48889017 ID=MS.gene071152
chr7.3 mRNA 48889017 48890543 48889017 ID=MS.gene071152.t1;Parent=MS.gene071152
chr7.3 exon 48890373 48890543 48890373 ID=MS.gene071152.t1.exon1;Parent=MS.gene071152.t1
chr7.3 CDS 48890373 48890543 48890373 ID=cds.MS.gene071152.t1;Parent=MS.gene071152.t1
chr7.3 exon 48890121 48890236 48890121 ID=MS.gene071152.t1.exon2;Parent=MS.gene071152.t1
chr7.3 CDS 48890121 48890236 48890121 ID=cds.MS.gene071152.t1;Parent=MS.gene071152.t1
chr7.3 exon 48889339 48889412 48889339 ID=MS.gene071152.t1.exon3;Parent=MS.gene071152.t1
chr7.3 CDS 48889339 48889412 48889339 ID=cds.MS.gene071152.t1;Parent=MS.gene071152.t1
chr7.3 exon 48889143 48889206 48889143 ID=MS.gene071152.t1.exon4;Parent=MS.gene071152.t1
chr7.3 CDS 48889143 48889206 48889143 ID=cds.MS.gene071152.t1;Parent=MS.gene071152.t1
chr7.3 exon 48889017 48889053 48889017 ID=MS.gene071152.t1.exon5;Parent=MS.gene071152.t1
chr7.3 CDS 48889017 48889053 48889017 ID=cds.MS.gene071152.t1;Parent=MS.gene071152.t1
Gene Sequence

>MS.gene071152

ATGAAGTTGATATTCTTGGGAAGCTCATTTTCTATTGTATGGTACATGAGGTACCACAAGATTGTTCGTAGATCATATGACAAAGATCAGGATACCTTCCGTCACTATTTTCTAGTATTGCCCTGCTTATTGTTGGCCCTACTTATCAATGAGAAATTCACATTTAAGGAGGTTAGAATGCATTCACTGTTGTCTATCTTCTGTAACTGTTGTGCTGCCATACATGTTTGAATGTTTTTAAGAGATGATGACTTTGCATTAATTTAGCTTTCGTTTGTTGTCCCAAAACTGCTTATACATGGAATAGGTGATGTGGACATTTTCCCTGTATTTGGAAGCTGTTGCCATACTTCCTCAACTGGTACTGCTACAAAGAACTAGAAACATTGACAATTTAACAGGACAATATGTCTTTCTTCTTGGGTAAGCTATGGTCCTTTAACATTATATATTTGCATGATTTCTGTGGCTGTTATTGCATTAGTATTGAAATTAGCAGCTATTATATTCTGGAAGTATTTCAGTTACTGATCAACAGCCAAATAACTGGAAGGTTGGTCTATGTTGATATCTATTTCTGCATAGAACGAGCTTAGTAGTGGTTTCATGCATGATAATGATGATGTAAATCGTAGTTGCAATTTGATACAGTTCTGAGTCATTGAACTAATGCTCTTTAAATAGTGATTCACATGAAGAAAATCATAGCATTTCATGTAACATGTTGTATAGGTTCCAAAGTTGTCAATCCAACTCACCTAGATAAATTATAGCTAATGGGGACAACAAAAGGAATAGAAAAATTATTAGTTTGCAGTAGTGCTCGTGGACAAGCCTGTTTGTTGGCTTCATTGAAACTGATCAATGAGACTGGCCTTCTATTATGATCTTGAGTTGAAAGTTTTTATTTGTTGGAACGAAACTGGCCTTCTATTATGATCTTGAAGCCAAAGTTCTGCTTATTAAGGTTTTCAAGTTTATGCAACTAGCTCTACATATAGTGTGCTATAATAAAAAATAAGCAATGGTAACTTTGTATATCAGAGGAACCCATTTGAACTTGTTTTAGCTTTTAGGCTTTAAGATCTTAGAATTCTGATTCAATAATATTGCTTTGTCTGTGAAATGCAGTGGGTACAGAGCACTATACATTCTCAACTGGGTCTATCGCTACTTTACCGAGCCCCACTTTGTCCATTGGATAAGTATAATTCTGTCTCTTGTTTAATTGTTATCATTGGTTTATGCTCTTTAGACTGGAAAACTTTTCCATCATTGTTAAACATTACAGTGGCTATAAACCTTGTTCTGACTTCCTTCCATTACTTCTTTTGCAGCATGGGTTTCTGGGCTTGTTCAAACACTGCTATATGCTGACTTCTTCTACTACTATTTCCAAAGGTAAATTGGCCTACTTGTCAGGTCCATTTATTTATGTTAATAATCCTTGTTCGTCTTAGTTTTGAGCATTTGAAAATATTGCCTTGTAGTTGGAAGAACAACCAGAAGCTTCATTTGCCAGCATAG

Protein sequence

>MS.gene071152.t1

MKLIFLGSSFSIVWYMRYHKIVRRSYDKDQDTFRHYFLVLPCLLLALLINEKFTFKEVMWTFSLYLEAVAILPQLVLLQRTRNIDNLTGQYVFLLGGYRALYILNWVYRYFTEPHFVHWITWVSGLVQTLLYADFFYYYFQSWKNNQKLHLPA