AlfalfaGEDB | MS.gene071560

M. sativa cultivar XinJiangDaYe / MS.gene071560

KEGG: -
GO: GO:0003674 GO:0005488 GO:0005515
PFAM: PF12796.10 PF13962.9

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene071560.t1	PNY06919.1	94.6	425	21	1	1	425	1	423	5.70E-126	461.1

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene071560.t1	Q6AWW5	56.5	255	94	3	174	425	284	524	4.1e-64	246.9

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene071560.t1	A0A2K3NV51	94.6	425	21	1	1	425	1	423	4.1e-126	461.1

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR

PPI:

Gene1	Gene2	Type
MS.gene05753	MS.gene071560	PPI
MS.gene38422	MS.gene071560	PPI
MS.gene071560	MS.gene41481	PPI
MS.gene071560	MS.gene068045	PPI
MS.gene071560	MS.gene90448	PPI
MS.gene071560	MS.gene049551	PPI
MS.gene071560	MS.gene023218	PPI
MS.gene071560	MS.gene48445	PPI
MS.gene071560	MS.gene008655	PPI
MS.gene41487	MS.gene071560	PPI
MS.gene071560	MS.gene27240	PPI
MS.gene071560	MS.gene051024	PPI
MS.gene071560	MS.gene23793	PPI
MS.gene071560	MS.gene69986	PPI
MS.gene015265	MS.gene071560	PPI
MS.gene071560	MS.gene92274	PPI
MS.gene071560	MS.gene008653	PPI
MS.gene071560	MS.gene02178	PPI
MS.gene89606	MS.gene071560	PPI
MS.gene071560	MS.gene41484	PPI
MS.gene071560	MS.gene032323	PPI
MS.gene071560	MS.gene44512	PPI
MS.gene071560	MS.gene073427	PPI
MS.gene38426	MS.gene071560	PPI
MS.gene071560	MS.gene03927	PPI
MS.gene071560	MS.gene01192	PPI
MS.gene071560	MS.gene053559	PPI
MS.gene071560	MS.gene066483	PPI
MS.gene071560	MS.gene008451	PPI
MS.gene071560	MS.gene008654	PPI
MS.gene071560	MS.gene35210	PPI
MS.gene071560	MS.gene03929	PPI
MS.gene071560	MS.gene08407	PPI
MS.gene071560	MS.gene99572	PPI
MS.gene049562	MS.gene071560	PPI
MS.gene071560	MS.gene049576	PPI
MS.gene071560	MS.gene017540	PPI
MS.gene049552	MS.gene071560	PPI
MS.gene071560	MS.gene90199	PPI
MS.gene071560	MS.gene35208	PPI
MS.gene071560	MS.gene023219	PPI
MS.gene38420	MS.gene071560	PPI
MS.gene071560	MS.gene028458	PPI
MS.gene071560	MS.gene41488	PPI
MS.gene071560	MS.gene013038	PPI
MS.gene071560	MS.gene008465	PPI
MS.gene071560	MS.gene41475	PPI
MS.gene071560	MS.gene048532	PPI
MS.gene071560	MS.gene007855	PPI
MS.gene071560	MS.gene008474	PPI
MS.gene071560	MS.gene41476	PPI
MS.gene071560	MS.gene27241	PPI
MS.gene071560	MS.gene20251	PPI
MS.gene071560	MS.gene20305	PPI
MS.gene23794	MS.gene071560	PPI
MS.gene09550	MS.gene071560	PPI
MS.gene071560	MS.gene38996	PPI
MS.gene071560	MS.gene25041	PPI
MS.gene071560	MS.gene38433	PPI
MS.gene071560	MS.gene38428	PPI
MS.gene071560	MS.gene41482	PPI
MS.gene071560	MS.gene05756	PPI
MS.gene071560	MS.gene028243	PPI
MS.gene071560	MS.gene43534	PPI
MS.gene071560	MS.gene09607	PPI
MS.gene071560	MS.gene008470	PPI
MS.gene071560	MS.gene049560	PPI
MS.gene071560	MS.gene017541	PPI
MS.gene071560	MS.gene020402	PPI
MS.gene071560	MS.gene76441	PPI
MS.gene071560	MS.gene03932	PPI
MS.gene071560	MS.gene38427	PPI
MS.gene071560	MS.gene028244	PPI
MS.gene071560	MS.gene32240	PPI
MS.gene071560	MS.gene23830	PPI
MS.gene071560	MS.gene015211	PPI
MS.gene071560	MS.gene05139	PPI
MS.gene049563	MS.gene071560	PPI
MS.gene071560	MS.gene048534	PPI
MS.gene071560	MS.gene78702	PPI
MS.gene071560	MS.gene05752	PPI
MS.gene071898	MS.gene071560	PPI
MS.gene071560	MS.gene81040	PPI
MS.gene071560	MS.gene041920	PPI
MS.gene071560	MS.gene05138	PPI
MS.gene011756	MS.gene071560	PPI
MS.gene071560	MS.gene049555	PPI
MS.gene071560	MS.gene08405	PPI
MS.gene008464	MS.gene071560	PPI
MS.gene071560	MS.gene008452	PPI
MS.gene071560	MS.gene38425	PPI

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene071560.t1	MTR_2g096190	97.412	425	10	1	1	425	121	544	0.0	840
MS.gene071560.t1	MTR_2g096190	31.788	151	102	1	17	166	68	218	1.23e-15	79.3
MS.gene071560.t1	MTR_4g062100	78.638	426	86	3	1	425	125	546	0.0	647
MS.gene071560.t1	MTR_4g062100	32.143	168	100	6	15	172	31	194	9.64e-11	63.9
MS.gene071560.t1	MTR_7g100390	65.566	424	138	4	2	425	105	520	0.0	528
MS.gene071560.t1	MTR_7g117620	59.479	422	155	4	5	425	140	546	3.42e-169	486
MS.gene071560.t1	MTR_7g117620	33.540	161	95	7	19	170	47	204	2.75e-11	65.9
MS.gene071560.t1	MTR_1g115575	48.499	433	190	5	1	425	186	593	1.16e-128	384
MS.gene071560.t1	MTR_1g032100	46.729	428	210	7	5	425	115	531	6.47e-125	372
MS.gene071560.t1	MTR_8g105260	45.455	429	214	8	5	425	122	538	2.89e-121	363
MS.gene071560.t1	MTR_7g100430	46.387	429	202	6	1	425	178	582	1.32e-106	327
MS.gene071560.t1	MTR_2g101310	25.358	489	276	10	5	423	162	631	1.43e-35	139
MS.gene071560.t1	MTR_2g101310	27.485	171	124	0	1	171	90	260	8.04e-12	67.4
MS.gene071560.t1	MTR_1g069110	27.072	362	225	10	9	347	44	389	3.69e-27	113
MS.gene071560.t1	MTR_2g101320	24.408	422	236	10	5	355	162	571	1.61e-26	112
MS.gene071560.t1	MTR_2g101320	25.581	172	128	0	1	172	90	261	7.77e-12	67.8
MS.gene071560.t1	MTR_2g101310	25.538	325	168	7	5	261	162	480	2.16e-18	87.4
MS.gene071560.t1	MTR_2g101310	27.485	171	124	0	1	171	90	260	4.19e-11	65.1
MS.gene071560.t1	MTR_5g085830	25.199	377	226	11	12	357	306	657	4.97e-18	87.0
MS.gene071560.t1	MTR_5g085810	24.877	406	215	12	1	357	321	685	2.14e-17	85.1
MS.gene071560.t1	MTR_5g085480	25.916	382	234	10	12	357	113	481	1.94e-16	81.6
MS.gene071560.t1	MTR_5g085480	25.916	382	234	10	12	357	273	641	2.85e-16	81.6
MS.gene071560.t1	MTR_8g066670	22.558	430	239	12	1	390	1127	1502	5.41e-16	80.9
MS.gene071560.t1	MTR_5g043640	31.694	183	118	5	12	192	51	228	6.11e-16	77.4
MS.gene071560.t1	MTR_5g085500	32.530	166	105	3	11	169	1	166	1.24e-15	79.0
MS.gene071560.t1	MTR_8g080080	36.709	158	83	5	18	158	188	345	1.03e-14	76.6
MS.gene071560.t1	MTR_8g080080	26.667	165	105	3	12	160	218	382	2.63e-11	65.9
MS.gene071560.t1	MTR_3g105650	22.744	277	175	5	1	261	57	310	3.14e-14	74.3
MS.gene071560.t1	MTR_7g099570	31.548	168	105	4	13	171	39	205	1.75e-13	72.8
MS.gene071560.t1	MTR_7g099570	31.333	150	93	4	1	141	61	209	3.05e-12	68.9
MS.gene071560.t1	MTR_8g073260	24.542	273	169	6	1	261	63	310	2.27e-13	72.0
MS.gene071560.t1	MTR_2g033050	31.288	163	105	3	1	156	218	380	3.24e-13	71.2
MS.gene071560.t1	MTR_3g085830	41.121	107	62	1	18	124	185	290	3.66e-13	71.6
MS.gene071560.t1	MTR_3g085830	28.750	160	98	4	12	156	213	371	7.42e-12	67.8
MS.gene071560.t1	MTR_8g467780	23.103	290	166	6	1	263	63	322	1.03e-12	70.1
MS.gene071560.t1	MTR_8g467780	28.378	148	105	1	17	163	45	192	1.67e-11	66.2
MS.gene071560.t1	MTR_5g026550	37.903	124	68	4	8	124	22	143	1.31e-12	66.2
MS.gene071560.t1	MTR_4g113530	28.866	194	128	5	12	200	548	736	2.70e-12	69.3
MS.gene071560.t1	MTR_8g040930	23.214	336	205	10	1	309	11	320	5.72e-12	67.4
MS.gene071560.t1	MTR_8g041240	23.214	336	205	10	1	309	11	320	5.72e-12	67.4
MS.gene071560.t1	MTR_1g112830	27.684	177	119	3	2	171	115	289	1.71e-11	66.6
MS.gene071560.t1	MTR_6g022830	31.461	178	102	5	12	172	221	395	3.22e-11	65.9
MS.gene071560.t1	MTR_6g023070	31.461	178	102	5	12	172	158	332	3.50e-11	65.5
MS.gene071560.t1	MTR_8g062410	24.870	193	130	2	1	181	62	251	8.26e-11	63.9

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene071560.t1	AT1G07710	76.415	424	98	1	2	425	122	543	0.0	617
MS.gene071560.t1	AT1G07710	76.415	424	98	1	2	425	122	543	0.0	617
MS.gene071560.t1	AT5G60070	68.981	432	116	6	1	425	128	548	0.0	558
MS.gene071560.t1	AT5G02620	62.413	431	139	4	1	425	111	524	3.96e-176	503
MS.gene071560.t1	AT5G02620	62.413	431	139	4	1	425	111	524	3.96e-176	503
MS.gene071560.t1	AT5G02620	62.413	431	139	4	1	425	111	524	3.96e-176	503
MS.gene071560.t1	AT5G02620	59.251	454	139	5	1	425	111	547	7.42e-171	490
MS.gene071560.t1	AT5G02620	34.524	168	109	1	6	172	47	214	5.51e-17	83.6
MS.gene071560.t1	AT5G02620	59.251	454	139	5	1	425	111	547	7.42e-171	490
MS.gene071560.t1	AT5G02620	34.524	168	109	1	6	172	47	214	5.51e-17	83.6
MS.gene071560.t1	AT2G31820	59.953	427	161	5	1	425	244	662	8.73e-171	494
MS.gene071560.t1	AT1G05640	60.280	428	159	6	1	425	208	627	2.44e-167	484
MS.gene071560.t1	AT2G01680	46.500	400	198	4	1	394	114	503	2.89e-119	358
MS.gene071560.t1	AT3G12360	48.301	412	192	5	7	414	178	572	6.26e-119	358
MS.gene071560.t1	AT3G12360	48.301	412	192	5	7	414	190	584	9.14e-119	358
MS.gene071560.t1	AT3G09550	46.620	429	206	5	1	425	198	607	9.71e-110	336
MS.gene071560.t1	AT3G09550	46.620	429	206	5	1	425	198	607	9.71e-110	336
MS.gene071560.t1	AT3G09550	46.620	429	206	5	1	425	205	614	1.41e-109	335
MS.gene071560.t1	AT5G54710	29.078	423	275	12	1	411	228	637	2.44e-35	138
MS.gene071560.t1	AT5G54710	29.078	423	275	12	1	411	183	592	2.52e-35	138
MS.gene071560.t1	AT1G34050	26.209	393	259	8	1	385	170	539	1.78e-31	126
MS.gene071560.t1	AT1G34050	37.634	93	58	0	80	172	51	143	9.07e-11	64.3
MS.gene071560.t1	AT2G24600	25.485	412	262	15	1	381	152	549	1.28e-30	124
MS.gene071560.t1	AT2G24600	25.485	412	262	15	1	381	152	549	1.28e-30	124
MS.gene071560.t1	AT1G10340	25.238	420	283	12	1	396	148	560	5.07e-27	114
MS.gene071560.t1	AT1G10340	25.238	420	283	12	1	396	152	564	5.77e-27	113
MS.gene071560.t1	AT4G03460	27.027	296	172	8	17	306	267	524	7.81e-25	107
MS.gene071560.t1	AT4G03460	32.867	143	84	3	1	132	67	208	2.47e-11	66.2
MS.gene071560.t1	AT1G03670	25.000	364	207	8	17	349	251	579	8.97e-25	107
MS.gene071560.t1	AT4G03460	25.581	301	170	7	17	306	235	492	9.33e-25	107
MS.gene071560.t1	AT5G50140	23.246	499	267	11	1	403	47	525	1.53e-23	103
MS.gene071560.t1	AT4G03470	26.400	375	223	11	4	369	293	623	2.89e-20	94.0
MS.gene071560.t1	AT5G54700	24.925	333	192	8	41	368	23	302	3.07e-20	91.7
MS.gene071560.t1	AT4G03490	25.065	387	243	11	1	377	227	576	1.66e-19	91.3
MS.gene071560.t1	AT4G03490	25.065	387	243	11	1	377	217	566	1.78e-19	91.3
MS.gene071560.t1	AT4G03490	25.065	387	243	11	1	377	227	576	2.66e-19	90.9
MS.gene071560.t1	AT5G51160	23.925	372	222	9	11	338	43	397	7.86e-19	88.6
MS.gene071560.t1	AT4G14390	26.197	355	238	9	20	363	295	636	1.59e-18	88.6
MS.gene071560.t1	AT4G14390	26.197	355	238	9	20	363	276	617	2.17e-18	88.2
MS.gene071560.t1	AT4G14390	26.211	351	235	9	24	363	300	637	9.47e-18	86.3
MS.gene071560.t1	AT4G03480	26.433	314	182	6	5	306	377	653	1.03e-17	85.9
MS.gene071560.t1	AT4G03480	26.433	314	182	6	5	306	380	656	1.09e-17	85.9
MS.gene071560.t1	AT4G03500	23.398	359	250	8	19	364	251	597	1.59e-17	85.5
MS.gene071560.t1	AT4G03500	23.398	359	250	8	19	364	228	574	2.08e-17	85.1
MS.gene071560.t1	AT4G03500	23.398	359	250	8	19	364	228	574	2.08e-17	85.1
MS.gene071560.t1	AT4G10720	24.922	321	196	11	1	304	57	349	2.45e-17	84.0
MS.gene071560.t1	AT2G24600	25.862	290	183	10	1	266	152	433	7.51e-17	83.2
MS.gene071560.t1	AT2G24600	25.862	290	183	10	1	266	152	433	7.51e-17	83.2
MS.gene071560.t1	AT4G10720	26.715	277	166	9	1	261	57	312	1.70e-16	81.6
MS.gene071560.t1	AT4G05040	26.766	269	150	4	4	263	296	526	2.59e-16	81.6
MS.gene071560.t1	AT4G05040	26.766	269	150	4	4	263	296	526	2.59e-16	81.6
MS.gene071560.t1	AT4G05040	26.766	269	150	4	4	263	296	526	2.59e-16	81.6
MS.gene071560.t1	AT4G05040	26.766	269	150	4	4	263	296	526	2.59e-16	81.6
MS.gene071560.t1	AT4G05040	26.766	269	150	4	4	263	296	526	2.59e-16	81.6
MS.gene071560.t1	AT4G05040	26.766	269	150	4	4	263	296	526	2.59e-16	81.6
MS.gene071560.t1	AT4G05040	26.766	269	150	4	4	263	296	526	2.59e-16	81.6
MS.gene071560.t1	AT5G54700	23.919	347	199	9	46	368	34	339	8.44e-16	79.7
MS.gene071560.t1	AT3G04140	29.609	179	107	3	12	171	222	400	3.23e-15	78.2
MS.gene071560.t1	AT3G04140	38.318	107	64	1	18	122	192	298	3.64e-13	72.0
MS.gene071560.t1	AT3G04140	37.273	110	67	1	50	157	190	299	2.65e-11	65.9
MS.gene071560.t1	AT4G03490	21.760	409	234	11	11	377	154	518	1.05e-13	73.6
MS.gene071560.t1	AT4G03490	21.760	409	234	11	11	377	164	528	1.21e-13	73.6
MS.gene071560.t1	AT4G14390	26.984	252	167	6	20	260	276	521	4.26e-13	71.2
MS.gene071560.t1	AT5G15500	23.457	324	208	8	1	306	58	359	7.28e-13	70.5
MS.gene071560.t1	AT2G03430	30.682	176	115	5	19	192	51	221	3.06e-12	66.6
MS.gene071560.t1	AT4G14400	24.324	370	229	12	20	363	201	545	3.83e-12	68.6
MS.gene071560.t1	AT4G14400	24.324	370	229	12	20	363	201	545	3.83e-12	68.6
MS.gene071560.t1	AT4G14400	24.324	370	229	12	20	363	267	611	5.72e-12	68.2
MS.gene071560.t1	AT3G01750	27.950	161	100	3	12	156	222	382	2.20e-11	66.2
MS.gene071560.t1	AT3G01750	36.538	104	64	1	52	153	192	295	3.44e-11	65.9
MS.gene071560.t1	AT3G01750	27.950	161	100	3	12	156	222	382	2.20e-11	66.2
MS.gene071560.t1	AT3G01750	36.538	104	64	1	52	153	192	295	3.44e-11	65.9
MS.gene071560.t1	AT5G07840	40.196	102	57	2	6	103	22	123	2.61e-11	62.4
MS.gene071560.t1	AT1G14500	30.709	127	86	2	13	138	35	160	3.35e-11	65.1
MS.gene071560.t1	AT5G54610	26.316	190	123	4	5	177	25	214	4.90e-11	64.7

Find 81 sgRNAs with CRISPR-Local

Find 100 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
CTTTGGGAACTATGTGTTAT+TGG	0.157747	2.3:-25512012	MS.gene071560:CDS
TGCAGCAAGGACTGATAAAA+AGG	0.264101	2.3:-25513154	MS.gene071560:CDS
AACTATAATGGCTACAACTT+TGG	0.277317	2.3:-25512029	MS.gene071560:CDS
AAAATTATAAATGGTGCTTC+TGG	0.278164	2.3:+25512277	None:intergenic
TACCCGAGTTCTGGCTTTCC+TGG	0.287238	2.3:+25513005	None:intergenic
GTAGACACTGCTGAGAAAAT+AGG	0.288492	2.3:-25512661	MS.gene071560:CDS
CAGATTGTAAATATGCTTCT+AGG	0.300619	2.3:-25512733	MS.gene071560:CDS
ATCCCTTCTGGAATATTGTT+TGG	0.316664	2.3:+25512325	None:intergenic
TATTGCACTCTTCATTTCTC+TGG	0.325254	2.3:-25512239	MS.gene071560:CDS
AATACAAAGAAAATTATAAA+TGG	0.331303	2.3:+25512268	None:intergenic
TATGTAATTTACCCGAGTTC+TGG	0.335480	2.3:+25512996	None:intergenic
TGCAAAGCTGTTGTGTTTGA+TGG	0.339635	2.3:+25513342	None:intergenic
TTTGGGAACTATGTGTTATT+GGG	0.342224	2.3:-25512011	MS.gene071560:CDS
TGAGATAGTGAAATTCCTAT+TGG	0.358809	2.3:-25513295	MS.gene071560:CDS
GCATTGTCATTTGTTGTTGT+TGG	0.360348	2.3:-25512097	MS.gene071560:CDS
CTTCATTCTGCTGCAAGAAA+TGG	0.361031	2.3:-25513222	MS.gene071560:CDS
TCCACTGTCATTGACAATTC+AGG	0.372401	2.3:+25513366	None:intergenic
CATCAAACTTGAGGAACATT+AGG	0.373191	2.3:-25511967	MS.gene071560:CDS
ACTATAATGGCTACAACTTT+GGG	0.376333	2.3:-25512028	MS.gene071560:CDS
TCAACCATGTTTATTGTTGA+AGG	0.380744	2.3:+25513060	None:intergenic
TCACCAAGTATCATCCCTTC+TGG	0.381269	2.3:+25512313	None:intergenic
TCAGTCCTTGCTGCAACTCC+TGG	0.385882	2.3:+25513162	None:intergenic
GAACACCATGCTCTAATAGA+AGG	0.390495	2.3:+25512620	None:intergenic
CATTATAGTTGCTCCTATAA+TGG	0.392151	2.3:+25512042	None:intergenic
TGCTGCAAGAAATGGACATT+TGG	0.400973	2.3:-25513214	MS.gene071560:CDS
TCTGGCGTGTGTGTTCTAAT+TGG	0.405927	2.3:+25512503	None:intergenic
CATTATAGGAGCAACTATAA+TGG	0.420585	2.3:-25512041	MS.gene071560:CDS
ATTGGAAGCAGGAAGTAGTT+TGG	0.421802	2.3:-25513277	MS.gene071560:CDS
CTTCATTTCTCTGGCTGTTG+TGG	0.427415	2.3:-25512230	MS.gene071560:CDS
TAGACACTGCTGAGAAAATA+GGG	0.434367	2.3:-25512660	MS.gene071560:CDS
CTGCCATGTGAAGTGCTGTC+TGG	0.436714	2.3:+25513130	None:intergenic
GCACTTCTTGAGAAAGAGCC+AGG	0.460376	2.3:-25513180	MS.gene071560:CDS
GTAAGTGCTGTTAACAGATC+AGG	0.461810	2.3:-25512694	MS.gene071560:CDS
TGCTAAAAGTAACGGGAAAA+CGG	0.468457	2.3:-25513247	MS.gene071560:CDS
GAACATTAGGAAATCTTCAA+TGG	0.470623	2.3:-25511954	MS.gene071560:CDS
AAATATGCTTCTAGGACAAA+AGG	0.471296	2.3:-25512725	MS.gene071560:CDS
AGCACTTCACATGGCAGTAA+AGG	0.471764	2.3:-25513124	MS.gene071560:CDS
CAGACACGAAAACGTGTTCA+AGG	0.474794	2.3:-25512484	MS.gene071560:CDS
GGGACAAAATCTTGAGGTAG+TGG	0.477594	2.3:-25513103	MS.gene071560:CDS
CAACCATTGCTAAAAGTAAC+GGG	0.485668	2.3:-25513254	MS.gene071560:CDS
GCACTTCACATGGCAGTAAA+GGG	0.488917	2.3:-25513123	MS.gene071560:CDS
AACTACTTCCTGCTTCCAAT+AGG	0.491463	2.3:+25513280	None:intergenic
AATTAATAAGCTAATGTGGT+TGG	0.495846	2.3:-25512149	MS.gene071560:CDS
GGCTTGTGTGCTTATTTCTG+TGG	0.502072	2.3:-25512128	MS.gene071560:CDS
GTGAAATTCCTATTGGAAGC+AGG	0.510342	2.3:-25513288	MS.gene071560:CDS
ATTCCAGAAGGGATGATACT+TGG	0.510792	2.3:-25512316	MS.gene071560:CDS
CAACCAGGAAAGCCAGAACT+CGG	0.516470	2.3:-25513008	MS.gene071560:CDS
ATAGAAGGGCTTTAACATCC+TGG	0.524388	2.3:+25512635	None:intergenic
GCAACCATTGCTAAAAGTAA+CGG	0.526222	2.3:-25513255	MS.gene071560:CDS
ATAAACATGGTTGATACTAA+AGG	0.532093	2.3:-25513051	MS.gene071560:CDS
GGTCATTAAGCATCGAATTG+AGG	0.539272	2.3:-25511990	MS.gene071560:CDS
TGCAAGAAATGGACATTTGG+AGG	0.543958	2.3:-25513211	MS.gene071560:CDS
GTCATTTGTTGTTGTTGGAA+AGG	0.554079	2.3:-25512092	MS.gene071560:CDS
GTGCTTGTTGATCCTTGTGG+TGG	0.555571	2.3:+25512577	None:intergenic
AAGCAAAGCGAAGAAACAAA+TGG	0.557199	2.3:-25512179	MS.gene071560:CDS
TGTTGTGGTTGTTCAAACTT+CGG	0.558193	2.3:-25512215	MS.gene071560:CDS
GATCCAAACAATATTCCAGA+AGG	0.558398	2.3:-25512328	MS.gene071560:CDS
TGCTGAGAAAATAGGGAACC+AGG	0.569656	2.3:-25512653	MS.gene071560:CDS
GGATCATCAACAAATTGACC+AGG	0.588310	2.3:+25512346	None:intergenic
AACACCATGCTCTAATAGAA+GGG	0.595108	2.3:+25512621	None:intergenic
TGCTATTAGTACTGCCACAA+CGG	0.600090	2.3:+25512399	None:intergenic
AACCAGGAAAGCCAGAACTC+GGG	0.603570	2.3:-25513007	MS.gene071560:intron
CTATAATTAATAAGCTAATG+TGG	0.603869	2.3:-25512153	MS.gene071560:CDS
AAAGCCCTTCTATTAGAGCA+TGG	0.609187	2.3:-25512625	MS.gene071560:CDS
TCCTGAATTGTCAATGACAG+TGG	0.613702	2.3:-25513367	MS.gene071560:CDS
ATCAACAAGATGAATACAGA+AGG	0.614257	2.3:-25512448	MS.gene071560:CDS
GGGCCAGACAGCACTTCACA+TGG	0.614969	2.3:-25513133	MS.gene071560:CDS
AAGAGCCAGGAGTTGCAGCA+AGG	0.615235	2.3:-25513167	MS.gene071560:CDS
CAACATTGCATATAGCAACC+AGG	0.627104	2.3:-25513023	MS.gene071560:CDS
TTGCATACAGCTGCAACACA+AGG	0.627719	2.3:-25513324	MS.gene071560:CDS
GAATTGAGGCATCAAACTTG+AGG	0.637621	2.3:-25511976	MS.gene071560:CDS
ATCCAAACAATATTCCAGAA+GGG	0.645443	2.3:-25512327	MS.gene071560:CDS
AGATCCTTCAACAATAAACA+TGG	0.658313	2.3:-25513064	MS.gene071560:CDS
TGTGGCAGTACTAATAGCAA+CGG	0.666243	2.3:-25512395	MS.gene071560:CDS
AATAAACTCGACAACCGTTG+TGG	0.669177	2.3:-25512413	MS.gene071560:CDS
AGTAAAGGGACAAAATCTTG+AGG	0.673310	2.3:-25513109	MS.gene071560:CDS
GCTGTGCTTGTTGATCCTTG+TGG	0.678811	2.3:+25512574	None:intergenic
GATCATCAACAAATTGACCA+GGG	0.681393	2.3:+25512347	None:intergenic
CAAAGCGAAGAAACAAATGG+CGG	0.712434	2.3:-25512176	MS.gene071560:CDS
AAGTCTATAAAACCACCACA+AGG	0.715719	2.3:-25512589	MS.gene071560:CDS
TGTAAGCGACATAAAGCACG+AGG	0.760936	2.3:-25512533	MS.gene071560:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	AATACAAAGAAAATTATAAA+TGG	+	chr2.3:25512981-25513000	None:intergenic	10.0%
!!!	AATTATTTGAATTTGTTTTC+TGG	-	chr2.3:25512327-25512346	MS.gene071560:CDS	15.0%
!!	CTATAATTAATAAGCTAATG+TGG	-	chr2.3:25513093-25513112	MS.gene071560:CDS	20.0%
!	AAAATTATAAATGGTGCTTC+TGG	+	chr2.3:25512972-25512991	None:intergenic	25.0%
!	AAATTCCATAGACATATACT+AGG	-	chr2.3:25512283-25512302	MS.gene071560:CDS	25.0%
!	AATTAATAAGCTAATGTGGT+TGG	-	chr2.3:25513097-25513116	MS.gene071560:CDS	25.0%
!!	ATAAACATGGTTGATACTAA+AGG	-	chr2.3:25512195-25512214	MS.gene071560:CDS	25.0%
!!	GCAATTTTTGTTACCATTAT+AGG	-	chr2.3:25513191-25513210	MS.gene071560:CDS	25.0%
	AACTATAATGGCTACAACTT+TGG	-	chr2.3:25513217-25513236	MS.gene071560:CDS	30.0%
	ACTATAATGGCTACAACTTT+GGG	-	chr2.3:25513218-25513237	MS.gene071560:CDS	30.0%
	AGATCCTTCAACAATAAACA+TGG	-	chr2.3:25512182-25512201	MS.gene071560:CDS	30.0%
	ATAGACATATACTAGGTACT+AGG	-	chr2.3:25512290-25512309	MS.gene071560:CDS	30.0%
	ATCAACAAGATGAATACAGA+AGG	-	chr2.3:25512798-25512817	MS.gene071560:intron	30.0%
	ATCCAAACAATATTCCAGAA+GGG	-	chr2.3:25512919-25512938	MS.gene071560:intron	30.0%
	CAGATTGTAAATATGCTTCT+AGG	-	chr2.3:25512513-25512532	MS.gene071560:CDS	30.0%
	CATTATAGGAGCAACTATAA+TGG	-	chr2.3:25513205-25513224	MS.gene071560:CDS	30.0%
	CATTATAGTTGCTCCTATAA+TGG	+	chr2.3:25513207-25513226	None:intergenic	30.0%
	GAACATTAGGAAATCTTCAA+TGG	-	chr2.3:25513292-25513311	MS.gene071560:CDS	30.0%
	TAGACATATACTAGGTACTA+GGG	-	chr2.3:25512291-25512310	MS.gene071560:CDS	30.0%
	TAGTACCTAGTATATGTCTA+TGG	+	chr2.3:25512291-25512310	None:intergenic	30.0%
	TCAACCATGTTTATTGTTGA+AGG	+	chr2.3:25512189-25512208	None:intergenic	30.0%
	TGAGATAGTGAAATTCCTAT+TGG	-	chr2.3:25511951-25511970	MS.gene071560:CDS	30.0%
	TTGTTGGAAAGGAAGAAAAA+TGG	-	chr2.3:25513165-25513184	MS.gene071560:CDS	30.0%
!	TTTGGGAACTATGTGTTATT+GGG	-	chr2.3:25513235-25513254	MS.gene071560:CDS	30.0%
!!	AAATATGCTTCTAGGACAAA+AGG	-	chr2.3:25512521-25512540	MS.gene071560:CDS	30.0%
	AACACCATGCTCTAATAGAA+GGG	+	chr2.3:25512628-25512647	None:intergenic	35.0%
	AAGCAAAGCGAAGAAACAAA+TGG	-	chr2.3:25513067-25513086	MS.gene071560:CDS	35.0%
	AAGTCTATAAAACCACCACA+AGG	-	chr2.3:25512657-25512676	MS.gene071560:CDS	35.0%
	AAGTTGCAGAAAGTAGCTTA+TGG	+	chr2.3:25512408-25512427	None:intergenic	35.0%
	AGTAAAGGGACAAAATCTTG+AGG	-	chr2.3:25512137-25512156	MS.gene071560:CDS	35.0%
	CAACCATTGCTAAAAGTAAC+GGG	-	chr2.3:25511992-25512011	MS.gene071560:CDS	35.0%
	CATCAAACTTGAGGAACATT+AGG	-	chr2.3:25513279-25513298	MS.gene071560:CDS	35.0%
	GATCATCAACAAATTGACCA+GGG	+	chr2.3:25512902-25512921	None:intergenic	35.0%
	GATCCAAACAATATTCCAGA+AGG	-	chr2.3:25512918-25512937	MS.gene071560:intron	35.0%
	GCAACCATTGCTAAAAGTAA+CGG	-	chr2.3:25511991-25512010	MS.gene071560:CDS	35.0%
	TAGACACTGCTGAGAAAATA+GGG	-	chr2.3:25512586-25512605	MS.gene071560:CDS	35.0%
	TATGTAATTTACCCGAGTTC+TGG	+	chr2.3:25512253-25512272	None:intergenic	35.0%
	TATTGCACTCTTCATTTCTC+TGG	-	chr2.3:25513007-25513026	MS.gene071560:intron	35.0%
	TGGAAAGGAAGAAAAATGGT+TGG	-	chr2.3:25513169-25513188	MS.gene071560:CDS	35.0%
	TGTTGTGGTTGTTCAAACTT+CGG	-	chr2.3:25513031-25513050	MS.gene071560:CDS	35.0%
	TTGAAATCCGTCATTGACAA+TGG	-	chr2.3:25512432-25512451	MS.gene071560:CDS	35.0%
!	CTTTGGGAACTATGTGTTAT+TGG	-	chr2.3:25513234-25513253	MS.gene071560:CDS	35.0%
!	TGCTAAAAGTAACGGGAAAA+CGG	-	chr2.3:25511999-25512018	MS.gene071560:CDS	35.0%
!!	ATCCCTTCTGGAATATTGTT+TGG	+	chr2.3:25512924-25512943	None:intergenic	35.0%
!!	GCATTGTCATTTGTTGTTGT+TGG	-	chr2.3:25513149-25513168	MS.gene071560:CDS	35.0%
!!	GTCATTTGTTGTTGTTGGAA+AGG	-	chr2.3:25513154-25513173	MS.gene071560:CDS	35.0%
!!	TAAGCACTTCTGAAACTGTT+TGG	-	chr2.3:25512352-25512371	MS.gene071560:CDS	35.0%
!!	TATTGACATCTGTTTCTGAG+AGG	-	chr2.3:25512466-25512485	MS.gene071560:CDS	35.0%
!!!	TTTCCCGTTACTTTTAGCAA+TGG	+	chr2.3:25511998-25512017	None:intergenic	35.0%
	AAAGCCCTTCTATTAGAGCA+TGG	-	chr2.3:25512621-25512640	MS.gene071560:CDS	40.0%
	AACTACTTCCTGCTTCCAAT+AGG	+	chr2.3:25511969-25511988	None:intergenic	40.0%
	AATAAACTCGACAACCGTTG+TGG	-	chr2.3:25512833-25512852	MS.gene071560:intron	40.0%
	ATCCGTCATTGACAATGGAA+AGG	-	chr2.3:25512437-25512456	MS.gene071560:CDS	40.0%
	ATTCCAGAAGGGATGATACT+TGG	-	chr2.3:25512930-25512949	MS.gene071560:intron	40.0%
	ATTGGAAGCAGGAAGTAGTT+TGG	-	chr2.3:25511969-25511988	MS.gene071560:CDS	40.0%
	CAAAGCGAAGAAACAAATGG+CGG	-	chr2.3:25513070-25513089	MS.gene071560:CDS	40.0%
	CAACATTGCATATAGCAACC+AGG	-	chr2.3:25512223-25512242	MS.gene071560:CDS	40.0%
	CTTCATTCTGCTGCAAGAAA+TGG	-	chr2.3:25512024-25512043	MS.gene071560:CDS	40.0%
	GAACACCATGCTCTAATAGA+AGG	+	chr2.3:25512629-25512648	None:intergenic	40.0%
	GCAGCAAGGACTGATAAAAA+GGG	-	chr2.3:25512093-25512112	MS.gene071560:CDS	40.0%
	GGATCATCAACAAATTGACC+AGG	+	chr2.3:25512903-25512922	None:intergenic	40.0%
	GTAGACACTGCTGAGAAAAT+AGG	-	chr2.3:25512585-25512604	MS.gene071560:CDS	40.0%
	GTGAAATTCCTATTGGAAGC+AGG	-	chr2.3:25511958-25511977	MS.gene071560:CDS	40.0%
	TCCACTGTCATTGACAATTC+AGG	+	chr2.3:25511883-25511902	None:intergenic	40.0%
	TCCCTTTCCATTGTCAATGA+CGG	+	chr2.3:25512442-25512461	None:intergenic	40.0%
	TCCGTCATTGACAATGGAAA+GGG	-	chr2.3:25512438-25512457	MS.gene071560:CDS	40.0%
	TCCTGAATTGTCAATGACAG+TGG	-	chr2.3:25511879-25511898	MS.gene071560:CDS	40.0%
	TGCAAAGCTGTTGTGTTTGA+TGG	+	chr2.3:25511907-25511926	None:intergenic	40.0%
	TGCAAGAAATGGACATTTGG+AGG	-	chr2.3:25512035-25512054	MS.gene071560:CDS	40.0%
	TGCAGCAAGGACTGATAAAA+AGG	-	chr2.3:25512092-25512111	MS.gene071560:CDS	40.0%
	TGCTATTAGTACTGCCACAA+CGG	+	chr2.3:25512850-25512869	None:intergenic	40.0%
	TGCTGCAAGAAATGGACATT+TGG	-	chr2.3:25512032-25512051	MS.gene071560:CDS	40.0%
	TGTGGCAGTACTAATAGCAA+CGG	-	chr2.3:25512851-25512870	MS.gene071560:intron	40.0%
!	ATAGAAGGGCTTTAACATCC+TGG	+	chr2.3:25512614-25512633	None:intergenic	40.0%
!	GAATTGAGGCATCAAACTTG+AGG	-	chr2.3:25513270-25513289	MS.gene071560:CDS	40.0%
!	GTAAGTGCTGTTAACAGATC+AGG	-	chr2.3:25512552-25512571	MS.gene071560:CDS	40.0%
!!	GGTCATTAAGCATCGAATTG+AGG	-	chr2.3:25513256-25513275	MS.gene071560:CDS	40.0%
	AGCACTTCACATGGCAGTAA+AGG	-	chr2.3:25512122-25512141	MS.gene071560:CDS	45.0%
	CAGACACGAAAACGTGTTCA+AGG	-	chr2.3:25512762-25512781	MS.gene071560:intron	45.0%
	CTTCATTTCTCTGGCTGTTG+TGG	-	chr2.3:25513016-25513035	MS.gene071560:CDS	45.0%
	GCACTTCACATGGCAGTAAA+GGG	-	chr2.3:25512123-25512142	MS.gene071560:CDS	45.0%
	GGGACAAAATCTTGAGGTAG+TGG	-	chr2.3:25512143-25512162	MS.gene071560:CDS	45.0%
	TCACCAAGTATCATCCCTTC+TGG	+	chr2.3:25512936-25512955	None:intergenic	45.0%
	TCTGGCGTGTGTGTTCTAAT+TGG	+	chr2.3:25512746-25512765	None:intergenic	45.0%
	TGCTGAGAAAATAGGGAACC+AGG	-	chr2.3:25512593-25512612	MS.gene071560:CDS	45.0%
	TGTAAGCGACATAAAGCACG+AGG	-	chr2.3:25512713-25512732	MS.gene071560:CDS	45.0%
	TTGCATACAGCTGCAACACA+AGG	-	chr2.3:25511922-25511941	MS.gene071560:CDS	45.0%
!	CTTGAACACGTTTTCGTGTC+TGG	+	chr2.3:25512764-25512783	None:intergenic	45.0%
!!	GGCTTGTGTGCTTATTTCTG+TGG	-	chr2.3:25513118-25513137	MS.gene071560:CDS	45.0%
	AACCAGGAAAGCCAGAACTC+GGG	-	chr2.3:25512239-25512258	MS.gene071560:CDS	50.0%
	CAACCAGGAAAGCCAGAACT+CGG	-	chr2.3:25512238-25512257	MS.gene071560:CDS	50.0%
	GCACTTCTTGAGAAAGAGCC+AGG	-	chr2.3:25512066-25512085	MS.gene071560:CDS	50.0%
!	GCTGTGCTTGTTGATCCTTG+TGG	+	chr2.3:25512675-25512694	None:intergenic	50.0%
!	GTGCTTGTTGATCCTTGTGG+TGG	+	chr2.3:25512672-25512691	None:intergenic	50.0%
	AAGAGCCAGGAGTTGCAGCA+AGG	-	chr2.3:25512079-25512098	MS.gene071560:CDS	55.0%
	TCAGTCCTTGCTGCAACTCC+TGG	+	chr2.3:25512087-25512106	None:intergenic	55.0%
!	GCTGCGATTTTCACTGTCCC+TGG	-	chr2.3:25512882-25512901	MS.gene071560:intron	55.0%
!!	CTGCCATGTGAAGTGCTGTC+TGG	+	chr2.3:25512119-25512138	None:intergenic	55.0%
!!	TACCCGAGTTCTGGCTTTCC+TGG	+	chr2.3:25512244-25512263	None:intergenic	55.0%
	GGGCCAGACAGCACTTCACA+TGG	-	chr2.3:25512113-25512132	MS.gene071560:CDS	60.0%

Chromosome	Type	Strat	End	Strand	Name
chr2.3	gene	25511868	25513400	25511868	ID=MS.gene071560
chr2.3	mRNA	25511868	25513400	25511868	ID=MS.gene071560.t1;Parent=MS.gene071560
chr2.3	exon	25513008	25513400	25513008	ID=MS.gene071560.t1.exon1;Parent=MS.gene071560.t1
chr2.3	CDS	25513008	25513400	25513008	ID=cds.MS.gene071560.t1;Parent=MS.gene071560.t1
chr2.3	exon	25511868	25512752	25511868	ID=MS.gene071560.t1.exon2;Parent=MS.gene071560.t1
chr2.3	CDS	25511868	25512752	25511868	ID=cds.MS.gene071560.t1;Parent=MS.gene071560.t1

Gene Sequence

>MS.gene071560

ATGGAGGTTCATCCTGAATTGTCAATGACAGTGGATCCATCAAACACAACAGCTTTGCATACAGCTGCAACACAAGGACATATTGAGATAGTGAAATTCCTATTGGAAGCAGGAAGTAGTTTGGCAACCATTGCTAAAAGTAACGGGAAAACGGCTCTTCATTCTGCTGCAAGAAATGGACATTTGGAGGTTGTGAAAGCACTTCTTGAGAAAGAGCCAGGAGTTGCAGCAAGGACTGATAAAAAGGGCCAGACAGCACTTCACATGGCAGTAAAGGGACAAAATCTTGAGGTAGTGGAAGAGTTGATAAAAGCAGATCCTTCAACAATAAACATGGTTGATACTAAAGGAAACACAACATTGCATATAGCAACCAGGAAAGCCAGAACTCGGGTAAATTACATAATGCTTGTCAAAATTCCATAGACATATACTAGGTACTAGGGTCTGTTTGTATTGAATTATTTGAATTTGTTTTCTGGCATAAGCACTTCTGAAACTGTTTGGAAGTGCTTGTGAAAACAGCTTATGACACATCCATAAGCTACTTTCTGCAACTTAATTTTGAAATCCGTCATTGACAATGGAAAGGGAAAATTATTGACATCTGTTTCTGAGAGGCTAAAATATGTAAATTTGTTTGTGCAGATTGTAAATATGCTTCTAGGACAAAAGGAAACAAATGTAAGTGCTGTTAACAGATCAGGTGAAACAGCAGTAGACACTGCTGAGAAAATAGGGAACCAGGATGTTAAAGCCCTTCTATTAGAGCATGGTGTTCAGAGTGCAAAGTCTATAAAACCACCACAAGGATCAACAAGCACAGCTCGCGAGTTAAAGCAAACTGTAAGCGACATAAAGCACGAGGTACATTACCAATTAGAACACACACGCCAGACACGAAAACGTGTTCAAGGAATCGCAAAACGTATCAACAAGATGAATACAGAAGGACTCAACAATGCAATAAACTCGACAACCGTTGTGGCAGTACTAATAGCAACGGTTGCATTTGCTGCGATTTTCACTGTCCCTGGTCAATTTGTTGATGATCCAAACAATATTCCAGAAGGGATGATACTTGGTGAAGCAAATATATCTCCAGAAGCACCATTTATAATTTTCTTTGTATTTGATTCTATTGCACTCTTCATTTCTCTGGCTGTTGTGGTTGTTCAAACTTCGGTTGTCGTTATAGAAAGCAAAGCGAAGAAACAAATGGCGGCTATAATTAATAAGCTAATGTGGTTGGCTTGTGTGCTTATTTCTGTGGCATTTTTAGCATTGTCATTTGTTGTTGTTGGAAAGGAAGAAAAATGGTTGGCAATTTTTGTTACCATTATAGGAGCAACTATAATGGCTACAACTTTGGGAACTATGTGTTATTGGGTCATTAAGCATCGAATTGAGGCATCAAACTTGAGGAACATTAGGAAATCTTCAATGGAAAGCAAATCAAAGTCTTTTTCAGTGACAGCTTTGTCAGATTCTGAGCTAATGAACAATGAATTTAAGAAAATGTATGCTATTTAG

Protein sequence

>MS.gene071560.t1

MEVHPELSMTVDPSNTTALHTAATQGHIEIVKFLLEAGSSLATIAKSNGKTALHSAARNGHLEVVKALLEKEPGVAARTDKKGQTALHMAVKGQNLEVVEELIKADPSTINMVDTKGNTTLHIATRKARTRIVNMLLGQKETNVSAVNRSGETAVDTAEKIGNQDVKALLLEHGVQSAKSIKPPQGSTSTARELKQTVSDIKHEVHYQLEHTRQTRKRVQGIAKRINKMNTEGLNNAINSTTVVAVLIATVAFAAIFTVPGQFVDDPNNIPEGMILGEANISPEAPFIIFFVFDSIALFISLAVVVVQTSVVVIESKAKKQMAAIINKLMWLACVLISVAFLALSFVVVGKEEKWLAIFVTIIGATIMATTLGTMCYWVIKHRIEASNLRNIRKSSMESKSKSFSVTALSDSELMNNEFKKMYAI