Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene071670.t1 | GAU29362.1 | 98.9 | 92 | 1 | 0 | 1 | 92 | 23 | 114 | 2.20E-38 | 167.9 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene071670.t1 | A8IAP8 | 45.6 | 90 | 48 | 1 | 1 | 90 | 31 | 119 | 1.8e-09 | 63.2 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene071670.t1 | A0A2Z6MWZ4 | 98.9 | 92 | 1 | 0 | 1 | 92 | 23 | 114 | 1.6e-38 | 167.9 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049283 | MS.gene071670 | -0.805421 | 1.35E-49 | -1.69E-46 |
| MS.gene049419 | MS.gene071670 | 0.858909 | 5.96E-63 | -1.69E-46 |
| MS.gene049801 | MS.gene071670 | -0.80318 | 3.94E-49 | -1.69E-46 |
| MS.gene049983 | MS.gene071670 | -0.806688 | 7.29E-50 | -1.69E-46 |
| MS.gene050105 | MS.gene071670 | -0.849989 | 2.27E-60 | -1.69E-46 |
| MS.gene050361 | MS.gene071670 | -0.801323 | 9.50E-49 | -1.69E-46 |
| MS.gene050654 | MS.gene071670 | -0.82354 | 1.31E-53 | -1.69E-46 |
| MS.gene050826 | MS.gene071670 | -0.803385 | 3.57E-49 | -1.69E-46 |
| MS.gene051122 | MS.gene071670 | -0.855004 | 8.44E-62 | -1.69E-46 |
| MS.gene051275 | MS.gene071670 | -0.808397 | 3.16E-50 | -1.69E-46 |
| MS.gene051368 | MS.gene071670 | -0.807975 | 3.89E-50 | -1.69E-46 |
| MS.gene051793 | MS.gene071670 | 0.836965 | 6.87E-57 | -1.69E-46 |
| MS.gene051828 | MS.gene071670 | 0.828796 | 7.37E-55 | -1.69E-46 |
| MS.gene051924 | MS.gene071670 | 0.821706 | 3.50E-53 | -1.69E-46 |
| MS.gene051926 | MS.gene071670 | 0.829573 | 4.77E-55 | -1.69E-46 |
| MS.gene05216 | MS.gene071670 | -0.867495 | 1.31E-65 | -1.69E-46 |
| MS.gene052674 | MS.gene071670 | -0.895717 | 7.35E-76 | -1.69E-46 |
| MS.gene052675 | MS.gene071670 | -0.899179 | 2.56E-77 | -1.69E-46 |
| MS.gene052677 | MS.gene071670 | -0.901784 | 1.89E-78 | -1.69E-46 |
| MS.gene053726 | MS.gene071670 | -0.811744 | 6.01E-51 | -1.69E-46 |
| MS.gene054479 | MS.gene071670 | -0.852351 | 4.89E-61 | -1.69E-46 |
| MS.gene054950 | MS.gene071670 | 0.805309 | 1.42E-49 | -1.69E-46 |
| MS.gene055063 | MS.gene071670 | 0.801986 | 6.95E-49 | -1.69E-46 |
| MS.gene055701 | MS.gene071670 | -0.843199 | 1.62E-58 | -1.69E-46 |
| MS.gene055926 | MS.gene071670 | -0.800987 | 1.11E-48 | -1.69E-46 |
| MS.gene056045 | MS.gene071670 | -0.809455 | 1.88E-50 | -1.69E-46 |
| MS.gene056064 | MS.gene071670 | -0.833722 | 4.53E-56 | -1.69E-46 |
| MS.gene056480 | MS.gene071670 | -0.85128 | 9.84E-61 | -1.69E-46 |
| MS.gene056779 | MS.gene071670 | -0.824946 | 6.13E-54 | -1.69E-46 |
| MS.gene056873 | MS.gene071670 | -0.801073 | 1.07E-48 | -1.69E-46 |
| MS.gene057155 | MS.gene071670 | -0.810303 | 1.23E-50 | -1.69E-46 |
| MS.gene057156 | MS.gene071670 | 0.866146 | 3.53E-65 | -1.69E-46 |
| MS.gene058081 | MS.gene071670 | -0.849479 | 3.15E-60 | -1.69E-46 |
| MS.gene058223 | MS.gene071670 | 0.893423 | 6.37E-75 | -1.69E-46 |
| MS.gene058429 | MS.gene071670 | -0.827696 | 1.36E-54 | -1.69E-46 |
| MS.gene059438 | MS.gene071670 | -0.818231 | 2.18E-52 | -1.69E-46 |
| MS.gene059439 | MS.gene071670 | -0.802621 | 5.14E-49 | -1.69E-46 |
| MS.gene059440 | MS.gene071670 | -0.827525 | 1.49E-54 | -1.69E-46 |
| MS.gene059952 | MS.gene071670 | -0.835646 | 1.49E-56 | -1.69E-46 |
| MS.gene06052 | MS.gene071670 | -0.826414 | 2.75E-54 | -1.69E-46 |
| MS.gene060850 | MS.gene071670 | -0.876022 | 1.94E-68 | -1.69E-46 |
| MS.gene060851 | MS.gene071670 | -0.891872 | 2.67E-74 | -1.69E-46 |
| MS.gene060852 | MS.gene071670 | -0.887924 | 9.30E-73 | -1.69E-46 |
| MS.gene060932 | MS.gene071670 | -0.837279 | 5.71E-57 | -1.69E-46 |
| MS.gene06121 | MS.gene071670 | -0.832398 | 9.67E-56 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene071670.t1 | MTR_1g114200 | 98.913 | 92 | 1 | 0 | 1 | 92 | 23 | 114 | 3.35e-61 | 182 |
| MS.gene071670.t1 | MTR_4g032660 | 33.708 | 89 | 59 | 0 | 1 | 89 | 108 | 196 | 1.27e-12 | 60.8 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene071670.t1 | AT5G27820 | 78.261 | 92 | 20 | 0 | 1 | 92 | 23 | 114 | 2.13e-50 | 154 |
| MS.gene071670.t1 | AT3G20230 | 35.955 | 89 | 57 | 0 | 1 | 89 | 95 | 183 | 1.50e-14 | 65.5 |
Find 23 sgRNAs with CRISPR-Local
Find 23 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CAACATCCCGAGTAGTTTCC+AGG | 0.336475 | 1.3:+77288396 | None:intergenic |
| AAGCGGTTATTGATTCTCTC+AGG | 0.400454 | 1.3:-77288265 | MS.gene071670:CDS |
| GAAAGCCTTGAGATCAAGCC+TGG | 0.444737 | 1.3:-77288414 | MS.gene071670:CDS |
| CTCCTGTGAGCTTGCAGAAG+AGG | 0.447057 | 1.3:+77288436 | None:intergenic |
| ATTGATTCTCTCAGGGAAGC+TGG | 0.452667 | 1.3:-77288257 | MS.gene071670:CDS |
| AAGAGAGAACAGAGGTATCA+TGG | 0.461247 | 1.3:-77288296 | MS.gene071670:CDS |
| GTTGCTGCAGCTGCAAAGAT+TGG | 0.469361 | 1.3:-77288377 | MS.gene071670:CDS |
| AGTTTCCAGGCTTGATCTCA+AGG | 0.483054 | 1.3:+77288409 | None:intergenic |
| TGCCTCTTCTGCAAGCTCAC+AGG | 0.512391 | 1.3:-77288438 | MS.gene071670:CDS |
| GAAGAGGCAACGGTAGCAGT+TGG | 0.531232 | 1.3:+77288452 | None:intergenic |
| GATCAAGCCTGGAAACTACT+CGG | 0.540330 | 1.3:-77288403 | MS.gene071670:CDS |
| AGCGGAACGCCTTTCGCTCA+AGG | 0.547714 | 1.3:-77288345 | MS.gene071670:CDS |
| CAGGAATGTCCTTGAGCGAA+AGG | 0.550217 | 1.3:+77288336 | None:intergenic |
| TTGCTGCAGCTGCAAAGATT+GGG | 0.550997 | 1.3:-77288376 | MS.gene071670:CDS |
| AGCGGTTATTGATTCTCTCA+GGG | 0.559363 | 1.3:-77288264 | MS.gene071670:CDS |
| TTCAAGTGAACAGAAACAGC+AGG | 0.564698 | 1.3:+77288317 | None:intergenic |
| AGAACAGAGGTATCATGGAA+AGG | 0.588862 | 1.3:-77288291 | MS.gene071670:CDS |
| ATCAAGCCTGGAAACTACTC+GGG | 0.593151 | 1.3:-77288402 | MS.gene071670:CDS |
| CAACGGTAGCAGTTGGAGTG+TGG | 0.604975 | 1.3:+77288459 | None:intergenic |
| TGAGCTTGCAGAAGAGGCAA+CGG | 0.638179 | 1.3:+77288442 | None:intergenic |
| GTATCATGGAAAGGTAAAAG+CGG | 0.664247 | 1.3:-77288282 | MS.gene071670:CDS |
| AAAGATTGGGAAGCTACTAG+CGG | 0.716694 | 1.3:-77288363 | MS.gene071670:CDS |
| TTCACTTGAAGAGAGAACAG+AGG | 0.737493 | 1.3:-77288304 | MS.gene071670:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| GTATCATGGAAAGGTAAAAG+CGG | - | chr1.3:77288456-77288475 | MS.gene071670:CDS | 35.0% | |
| AAAGATTGGGAAGCTACTAG+CGG | - | chr1.3:77288375-77288394 | MS.gene071670:CDS | 40.0% | |
| TTCAAGTGAACAGAAACAGC+AGG | + | chr1.3:77288424-77288443 | None:intergenic | 40.0% | |
| TTCACTTGAAGAGAGAACAG+AGG | - | chr1.3:77288434-77288453 | MS.gene071670:CDS | 40.0% | |
| !! | AAGAGAGAACAGAGGTATCA+TGG | - | chr1.3:77288442-77288461 | MS.gene071670:CDS | 40.0% |
| !! | AAGCGGTTATTGATTCTCTC+AGG | - | chr1.3:77288473-77288492 | MS.gene071670:CDS | 40.0% |
| !! | AGAACAGAGGTATCATGGAA+AGG | - | chr1.3:77288447-77288466 | MS.gene071670:CDS | 40.0% |
| !! | AGCGGTTATTGATTCTCTCA+GGG | - | chr1.3:77288474-77288493 | MS.gene071670:CDS | 40.0% |
| AGTTTCCAGGCTTGATCTCA+AGG | + | chr1.3:77288332-77288351 | None:intergenic | 45.0% | |
| ATCAAGCCTGGAAACTACTC+GGG | - | chr1.3:77288336-77288355 | MS.gene071670:CDS | 45.0% | |
| GATCAAGCCTGGAAACTACT+CGG | - | chr1.3:77288335-77288354 | MS.gene071670:CDS | 45.0% | |
| TTGCTGCAGCTGCAAAGATT+GGG | - | chr1.3:77288362-77288381 | MS.gene071670:CDS | 45.0% | |
| ! | ATTGATTCTCTCAGGGAAGC+TGG | - | chr1.3:77288481-77288500 | MS.gene071670:CDS | 45.0% |
| CAACATCCCGAGTAGTTTCC+AGG | + | chr1.3:77288345-77288364 | None:intergenic | 50.0% | |
| CAGGAATGTCCTTGAGCGAA+AGG | + | chr1.3:77288405-77288424 | None:intergenic | 50.0% | |
| GAAAGCCTTGAGATCAAGCC+TGG | - | chr1.3:77288324-77288343 | MS.gene071670:CDS | 50.0% | |
| GTTGCTGCAGCTGCAAAGAT+TGG | - | chr1.3:77288361-77288380 | MS.gene071670:CDS | 50.0% | |
| TGAGCTTGCAGAAGAGGCAA+CGG | + | chr1.3:77288299-77288318 | None:intergenic | 50.0% | |
| CAACGGTAGCAGTTGGAGTG+TGG | + | chr1.3:77288282-77288301 | None:intergenic | 55.0% | |
| CTCCTGTGAGCTTGCAGAAG+AGG | + | chr1.3:77288305-77288324 | None:intergenic | 55.0% | |
| GAAGAGGCAACGGTAGCAGT+TGG | + | chr1.3:77288289-77288308 | None:intergenic | 55.0% | |
| TGCCTCTTCTGCAAGCTCAC+AGG | - | chr1.3:77288300-77288319 | MS.gene071670:CDS | 55.0% | |
| AGCGGAACGCCTTTCGCTCA+AGG | - | chr1.3:77288393-77288412 | MS.gene071670:CDS | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr1.3 | gene | 77288241 | 77288519 | 77288241 | ID=MS.gene071670 |
| chr1.3 | mRNA | 77288241 | 77288519 | 77288241 | ID=MS.gene071670.t1;Parent=MS.gene071670 |
| chr1.3 | exon | 77288241 | 77288519 | 77288241 | ID=MS.gene071670.t1.exon1;Parent=MS.gene071670.t1 |
| chr1.3 | CDS | 77288241 | 77288519 | 77288241 | ID=cds.MS.gene071670.t1;Parent=MS.gene071670.t1 |
| Gene Sequence |
| Protein sequence |
