AlfalfaGEDB | MS.gene09052

M. sativa cultivar XinJiangDaYe / MS.gene09052

KEGG: K00454
GO: -
PFAM: -

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene09052.t1	XP_013446534.1	85.9	135	15	1	1	131	1	135	1.30E-47	199.1

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene09052.t1	Q9CAG3	36.8	117	66	2	10	124	16	126	4.8e-06	52.4

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene09052.t1	A0A072TTP2	85.9	135	15	1	1	131	1	135	9.4e-48	199.1

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene049356	MS.gene09052	0.829047	6.40E-55	-1.69E-46
MS.gene049369	MS.gene09052	0.804862	1.76E-49	-1.69E-46
MS.gene049907	MS.gene09052	0.820494	6.66E-53	-1.69E-46
MS.gene050122	MS.gene09052	0.973222	4.60E-136	-1.69E-46
MS.gene050520	MS.gene09052	0.81588	7.37E-52	-1.69E-46
MS.gene050670	MS.gene09052	0.805352	1.39E-49	-1.69E-46
MS.gene05105	MS.gene09052	0.812499	4.11E-51	-1.69E-46
MS.gene051255	MS.gene09052	0.819504	1.12E-52	-1.69E-46
MS.gene051360	MS.gene09052	0.823433	1.39E-53	-1.69E-46
MS.gene052346	MS.gene09052	0.816838	4.50E-52	-1.69E-46
MS.gene052414	MS.gene09052	0.835311	1.81E-56	-1.69E-46
MS.gene052485	MS.gene09052	0.818056	2.39E-52	-1.69E-46
MS.gene052711	MS.gene09052	0.81353	2.44E-51	-1.69E-46
MS.gene052982	MS.gene09052	0.800625	1.32E-48	-1.69E-46
MS.gene052999	MS.gene09052	0.815192	1.05E-51	-1.69E-46
MS.gene053169	MS.gene09052	0.825606	4.28E-54	-1.69E-46
MS.gene053318	MS.gene09052	0.80595	1.04E-49	-1.69E-46
MS.gene053813	MS.gene09052	0.81863	1.77E-52	-1.69E-46
MS.gene054010	MS.gene09052	0.812649	3.81E-51	-1.69E-46
MS.gene054183	MS.gene09052	0.806278	8.89E-50	-1.69E-46
MS.gene054556	MS.gene09052	0.814076	1.85E-51	-1.69E-46
MS.gene055116	MS.gene09052	0.801454	8.93E-49	-1.69E-46
MS.gene055369	MS.gene09052	0.819838	9.41E-53	-1.69E-46
MS.gene055741	MS.gene09052	0.825406	4.77E-54	-1.69E-46
MS.gene055744	MS.gene09052	0.825654	4.17E-54	-1.69E-46
MS.gene055909	MS.gene09052	0.849948	2.33E-60	-1.69E-46
MS.gene055911	MS.gene09052	0.854825	9.51E-62	-1.69E-46
MS.gene05601	MS.gene09052	0.825012	5.91E-54	-1.69E-46
MS.gene05602	MS.gene09052	0.815036	1.14E-51	-1.69E-46
MS.gene056346	MS.gene09052	0.819052	1.42E-52	-1.69E-46
MS.gene056514	MS.gene09052	0.804633	1.97E-49	-1.69E-46
MS.gene05666	MS.gene09052	0.821824	3.29E-53	-1.69E-46
MS.gene056902	MS.gene09052	0.819946	8.89E-53	-1.69E-46
MS.gene057019	MS.gene09052	0.80539	1.37E-49	-1.69E-46
MS.gene057289	MS.gene09052	0.809486	1.85E-50	-1.69E-46
MS.gene057734	MS.gene09052	0.805877	1.08E-49	-1.69E-46
MS.gene057749	MS.gene09052	0.832182	1.09E-55	-1.69E-46
MS.gene057823	MS.gene09052	0.836196	1.08E-56	-1.69E-46
MS.gene057826	MS.gene09052	0.809978	1.45E-50	-1.69E-46
MS.gene058120	MS.gene09052	0.822153	2.76E-53	-1.69E-46
MS.gene058948	MS.gene09052	0.862414	5.16E-64	-1.69E-46
MS.gene058949	MS.gene09052	0.829897	3.98E-55	-1.69E-46
MS.gene058995	MS.gene09052	0.800139	1.66E-48	-1.69E-46
MS.gene059367	MS.gene09052	0.808431	3.11E-50	-1.69E-46
MS.gene059368	MS.gene09052	0.84264	2.29E-58	-1.69E-46
MS.gene059420	MS.gene09052	0.84164	4.20E-58	-1.69E-46
MS.gene059517	MS.gene09052	0.849761	2.63E-60	-1.69E-46
MS.gene059674	MS.gene09052	0.811804	5.83E-51	-1.69E-46
MS.gene059680	MS.gene09052	0.830364	3.06E-55	-1.69E-46
MS.gene060372	MS.gene09052	0.841291	5.20E-58	-1.69E-46
MS.gene060835	MS.gene09052	0.801692	7.99E-49	-1.69E-46
MS.gene060837	MS.gene09052	0.846247	2.45E-59	-1.69E-46

PPI:

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene09052.t1	MTR_8g080230	89.600	125	13	0	1	125	1	125	2.02e-47	163

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene09052.t1	AT1G67560	39.516	124	53	4	10	124	16	126	1.43e-13	67.0

Find 37 sgRNAs with CRISPR-Local

Find 37 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
TAGTGTGGTTGAGGAGTTTG+AGG	0.343973	4.4:+16216603	MS.gene09052:CDS
TCTCCTCCGGTGCCTCTTAT+CGG	0.348322	4.4:-16216380	None:intergenic
ACGGCGGAACGTTCAAGTTC+AGG	0.407328	4.4:+16216423	MS.gene09052:CDS
TGAGGAGTTTGAGGATCAGT+TGG	0.424110	4.4:+16216612	MS.gene09052:CDS
TTCATCAATGGTGTTGGTCA+AGG	0.463683	4.4:+16216640	MS.gene09052:CDS
AATGAAAGTTAGTGTGGTTG+AGG	0.473822	4.4:+16216594	MS.gene09052:CDS
TTGCTCACTTTGCTGTCCAC+CGG	0.486780	4.4:-16216487	None:intergenic
CAGTTGGAGTATTTCATCAA+TGG	0.487178	4.4:+16216628	MS.gene09052:CDS
CGTCGGTATTCTCCGATAAG+AGG	0.494868	4.4:+16216368	MS.gene09052:CDS
CAAGACTACAACGTCGTCTC+CGG	0.510717	4.4:+16216468	MS.gene09052:CDS
GAGTATTTCATCAATGGTGT+TGG	0.511662	4.4:+16216634	MS.gene09052:CDS
TCATCAATGGTGTTGGTCAA+GGG	0.527017	4.4:+16216641	MS.gene09052:CDS
CGGAGAATACCGACGGCGAT+CGG	0.549502	4.4:-16216360	None:intergenic
AAGCAACGGTGAAATGATAG+AGG	0.555163	4.4:+16216543	MS.gene09052:CDS
ACCGTGAAATGATTCTCCTC+CGG	0.555957	4.4:-16216393	None:intergenic
AAAGAAAATGAAAGTTAGTG+TGG	0.562354	4.4:+16216588	MS.gene09052:CDS
ACCGGAGGAGAATCATTTCA+CGG	0.579496	4.4:+16216392	MS.gene09052:CDS
TCATTTCACGGTCATCTGAA+CGG	0.584499	4.4:+16216404	MS.gene09052:CDS
GACTACAACGTCGTCTCCGG+TGG	0.584840	4.4:+16216471	MS.gene09052:CDS
GTTAGTGAACTCATAGACCC+TGG	0.596456	4.4:+16216679	MS.gene09052:CDS
TACAAAGTAGCTCGAGTGGT+GGG	0.598092	4.4:+16216518	MS.gene09052:CDS
GACGGCGATCGGAGGAGTAA+CGG	0.598155	4.4:-16216349	None:intergenic
TCGAGTGGTGGGAGAAGCAA+CGG	0.607804	4.4:+16216529	MS.gene09052:CDS
TTACAAAGTAGCTCGAGTGG+TGG	0.614453	4.4:+16216517	MS.gene09052:CDS
TATTCTCCGATAAGAGGCAC+CGG	0.616121	4.4:+16216374	MS.gene09052:CDS
TATGAGTTCACTAACAAACC+AGG	0.617835	4.4:-16216697	None:intergenic
GTGGACAGCAAAGTGAGCAA+TGG	0.630531	4.4:+16216490	MS.gene09052:CDS
GTTACTCCTCCGATCGCCGT+CGG	0.630592	4.4:+16216351	MS.gene09052:CDS
AGAATACCGACGGCGATCGG+AGG	0.636190	4.4:-16216357	None:intergenic
TCGTTACAAAGTAGCTCGAG+TGG	0.638320	4.4:+16216514	MS.gene09052:CDS
CTCTTATCGGAGAATACCGA+CGG	0.638821	4.4:-16216367	None:intergenic
ATGAGTTCACTAACAAACCA+GGG	0.645427	4.4:-16216696	None:intergenic
CAAGTTCAGGCAGTGATCAG+CGG	0.651845	4.4:+16216436	MS.gene09052:CDS
GTTCAGGCAGTGATCAGCGG+CGG	0.669499	4.4:+16216439	MS.gene09052:CDS
TCTCCGATAAGAGGCACCGG+AGG	0.671014	4.4:+16216377	MS.gene09052:CDS
AATGATAGAGGTGAAAGCAG+TGG	0.733351	4.4:+16216555	MS.gene09052:CDS
TTTCACGGTCATCTGAACGG+CGG	0.762381	4.4:+16216407	MS.gene09052:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!	AAAGAAAATGAAAGTTAGTG+TGG	+	chr4.4:16216588-16216607	MS.gene09052:CDS	25.0%
	AATGAAAGTTAGTGTGGTTG+AGG	+	chr4.4:16216594-16216613	MS.gene09052:CDS	35.0%
	ATGAGTTCACTAACAAACCA+GGG	-	chr4.4:16216699-16216718	None:intergenic	35.0%
	GAGTATTTCATCAATGGTGT+TGG	+	chr4.4:16216634-16216653	MS.gene09052:CDS	35.0%
	TATGAGTTCACTAACAAACC+AGG	-	chr4.4:16216700-16216719	None:intergenic	35.0%
!	CAGTTGGAGTATTTCATCAA+TGG	+	chr4.4:16216628-16216647	MS.gene09052:CDS	35.0%
	AAGCAACGGTGAAATGATAG+AGG	+	chr4.4:16216543-16216562	MS.gene09052:CDS	40.0%
	AATGATAGAGGTGAAAGCAG+TGG	+	chr4.4:16216555-16216574	MS.gene09052:CDS	40.0%
	TCATTTCACGGTCATCTGAA+CGG	+	chr4.4:16216404-16216423	MS.gene09052:CDS	40.0%
!!	TCATCAATGGTGTTGGTCAA+GGG	+	chr4.4:16216641-16216660	MS.gene09052:CDS	40.0%
!!	TTCATCAATGGTGTTGGTCA+AGG	+	chr4.4:16216640-16216659	MS.gene09052:CDS	40.0%
	ACCGGAGGAGAATCATTTCA+CGG	+	chr4.4:16216392-16216411	MS.gene09052:CDS	45.0%
	ACCGTGAAATGATTCTCCTC+CGG	-	chr4.4:16216396-16216415	None:intergenic	45.0%
	CTCTTATCGGAGAATACCGA+CGG	-	chr4.4:16216370-16216389	None:intergenic	45.0%
	GTTAGTGAACTCATAGACCC+TGG	+	chr4.4:16216679-16216698	MS.gene09052:CDS	45.0%
	TACAAAGTAGCTCGAGTGGT+GGG	+	chr4.4:16216518-16216537	MS.gene09052:CDS	45.0%
	TAGTGTGGTTGAGGAGTTTG+AGG	+	chr4.4:16216603-16216622	MS.gene09052:CDS	45.0%
	TCGTTACAAAGTAGCTCGAG+TGG	+	chr4.4:16216514-16216533	MS.gene09052:CDS	45.0%
	TGAGGAGTTTGAGGATCAGT+TGG	+	chr4.4:16216612-16216631	MS.gene09052:CDS	45.0%
	TTACAAAGTAGCTCGAGTGG+TGG	+	chr4.4:16216517-16216536	MS.gene09052:CDS	45.0%
!	TATTCTCCGATAAGAGGCAC+CGG	+	chr4.4:16216374-16216393	MS.gene09052:CDS	45.0%
	CAAGACTACAACGTCGTCTC+CGG	+	chr4.4:16216468-16216487	MS.gene09052:CDS	50.0%
	CAAGTTCAGGCAGTGATCAG+CGG	+	chr4.4:16216436-16216455	MS.gene09052:CDS	50.0%
	CGTCGGTATTCTCCGATAAG+AGG	+	chr4.4:16216368-16216387	MS.gene09052:CDS	50.0%
	GTGGACAGCAAAGTGAGCAA+TGG	+	chr4.4:16216490-16216509	MS.gene09052:CDS	50.0%
	TTTCACGGTCATCTGAACGG+CGG	+	chr4.4:16216407-16216426	MS.gene09052:CDS	50.0%
!	TTGCTCACTTTGCTGTCCAC+CGG	-	chr4.4:16216490-16216509	None:intergenic	50.0%
	TCTCCTCCGGTGCCTCTTAT+CGG	-	chr4.4:16216383-16216402	None:intergenic	55.0%
!	ACGGCGGAACGTTCAAGTTC+AGG	+	chr4.4:16216423-16216442	MS.gene09052:CDS	55.0%
!	TCGAGTGGTGGGAGAAGCAA+CGG	+	chr4.4:16216529-16216548	MS.gene09052:CDS	55.0%
	AGAATACCGACGGCGATCGG+AGG	-	chr4.4:16216360-16216379	None:intergenic	60.0%
	CGGAGAATACCGACGGCGAT+CGG	-	chr4.4:16216363-16216382	None:intergenic	60.0%
	GACGGCGATCGGAGGAGTAA+CGG	-	chr4.4:16216352-16216371	None:intergenic	60.0%
	GACTACAACGTCGTCTCCGG+TGG	+	chr4.4:16216471-16216490	MS.gene09052:CDS	60.0%
	GTTACTCCTCCGATCGCCGT+CGG	+	chr4.4:16216351-16216370	MS.gene09052:CDS	60.0%
	GTTCAGGCAGTGATCAGCGG+CGG	+	chr4.4:16216439-16216458	MS.gene09052:CDS	60.0%
!!	TCTCCGATAAGAGGCACCGG+AGG	+	chr4.4:16216377-16216396	MS.gene09052:CDS	60.0%

Chromosome	Type	Strat	End	Strand	Name
chr4.4	gene	16216328	16216744	16216328	ID=MS.gene09052
chr4.4	mRNA	16216328	16216744	16216328	ID=MS.gene09052.t1;Parent=MS.gene09052
chr4.4	exon	16216328	16216744	16216328	ID=MS.gene09052.t1.exon1;Parent=MS.gene09052.t1
chr4.4	CDS	16216328	16216744	16216328	ID=cds.MS.gene09052.t1;Parent=MS.gene09052.t1

Gene Sequence

>MS.gene09052

ATGTTCGCAGTGAAGTCTAATCCGTTACTCCTCCGATCGCCGTCGGTATTCTCCGATAAGAGGCACCGGAGGAGAATCATTTCACGGTCATCTGAACGGCGGAACGTTCAAGTTCAGGCAGTGATCAGCGGCGGAGACAGCAAGACTACAACGTCGTCTCCGGTGGACAGCAAAGTGAGCAATGGCTCGTTACAAAGTAGCTCGAGTGGTGGGAGAAGCAACGGTGAAATGATAGAGGTGAAAGCAGTGGTGATGATAAGAAAGAAAATGAAAGTTAGTGTGGTTGAGGAGTTTGAGGATCAGTTGGAGTATTTCATCAATGGTGTTGGTCAAGGGATACAGATTCAGCTTGTTAGTGAACTCATAGACCCTGGTTTGTTAGTGAACTCATATTATTTTCTCTATTCTTCTATATAA

Protein sequence

>MS.gene09052.t1

MFAVKSNPLLLRSPSVFSDKRHRRRIISRSSERRNVQVQAVISGGDSKTTTSSPVDSKVSNGSLQSSSSGGRSNGEMIEVKAVVMIRKKMKVSVVEEFEDQLEYFINGVGQGIQIQLVSELIDPGLLVNSYYFLYSSI