AlfalfaGEDB | MS.gene23378

M. sativa cultivar XinJiangDaYe / MS.gene23378

KEGG: -
GO: -
PFAM: -

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene23378.t1	XP_003622774.1	100	45	0	0	8	52	1	45	4.70E-14	86.3

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene23378.t1	G7KY15	100.0	45	0	0	8	52	1	45	3.4e-14	86.3

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene049758	MS.gene23378	0.879207	1.50E-69	-1.69E-46
MS.gene049915	MS.gene23378	0.829574	4.77E-55	-1.69E-46
MS.gene050099	MS.gene23378	0.833765	4.42E-56	-1.69E-46
MS.gene050301	MS.gene23378	0.831064	2.06E-55	-1.69E-46
MS.gene051471	MS.gene23378	0.828948	6.77E-55	-1.69E-46
MS.gene051662	MS.gene23378	0.851737	7.30E-61	-1.69E-46
MS.gene051663	MS.gene23378	0.857152	1.98E-62	-1.69E-46
MS.gene052078	MS.gene23378	0.813586	2.38E-51	-1.69E-46
MS.gene052207	MS.gene23378	0.802559	5.30E-49	-1.69E-46
MS.gene053836	MS.gene23378	0.868803	4.98E-66	-1.69E-46
MS.gene054184	MS.gene23378	0.810594	1.07E-50	-1.69E-46
MS.gene054185	MS.gene23378	0.818272	2.14E-52	-1.69E-46
MS.gene054473	MS.gene23378	0.804788	1.83E-49	-1.69E-46
MS.gene054667	MS.gene23378	0.829606	4.69E-55	-1.69E-46
MS.gene055133	MS.gene23378	0.85465	1.07E-61	-1.69E-46
MS.gene055277	MS.gene23378	0.830207	3.34E-55	-1.69E-46
MS.gene055390	MS.gene23378	0.822348	2.48E-53	-1.69E-46
MS.gene055422	MS.gene23378	0.869959	2.09E-66	-1.69E-46
MS.gene055428	MS.gene23378	0.819238	1.29E-52	-1.69E-46
MS.gene055509	MS.gene23378	0.801104	1.05E-48	-1.69E-46
MS.gene055712	MS.gene23378	0.802517	5.40E-49	-1.69E-46
MS.gene056069	MS.gene23378	-0.816394	5.66E-52	-1.69E-46
MS.gene056875	MS.gene23378	0.805834	1.10E-49	-1.69E-46
MS.gene057121	MS.gene23378	0.812287	4.57E-51	-1.69E-46
MS.gene057260	MS.gene23378	0.823778	1.15E-53	-1.69E-46
MS.gene057439	MS.gene23378	0.852687	3.92E-61	-1.69E-46
MS.gene057754	MS.gene23378	0.837795	4.21E-57	-1.69E-46
MS.gene058002	MS.gene23378	0.83872	2.43E-57	-1.69E-46
MS.gene05811	MS.gene23378	0.833212	6.07E-56	-1.69E-46
MS.gene058182	MS.gene23378	0.800325	1.52E-48	-1.69E-46
MS.gene058660	MS.gene23378	0.8032	3.91E-49	-1.69E-46
MS.gene058749	MS.gene23378	0.818306	2.10E-52	-1.69E-46
MS.gene058845	MS.gene23378	0.833345	5.63E-56	-1.69E-46
MS.gene05907	MS.gene23378	0.813846	2.08E-51	-1.69E-46
MS.gene059228	MS.gene23378	0.808507	2.99E-50	-1.69E-46
MS.gene059518	MS.gene23378	0.832842	7.50E-56	-1.69E-46
MS.gene059597	MS.gene23378	0.80104	1.09E-48	-1.69E-46
MS.gene05984	MS.gene23378	0.825465	4.62E-54	-1.69E-46
MS.gene059894	MS.gene23378	0.815329	9.78E-52	-1.69E-46
MS.gene06037	MS.gene23378	0.843718	1.18E-58	-1.69E-46
MS.gene060476	MS.gene23378	0.88777	1.07E-72	-1.69E-46
MS.gene061049	MS.gene23378	0.833211	6.08E-56	-1.69E-46
MS.gene061113	MS.gene23378	0.802706	4.94E-49	-1.69E-46

PPI:

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Find 9 sgRNAs with CRISPR-Local

Find 33 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAGAGTAGCATGAGTACTTT+TGG	0.271602	7.3:+52236518	None:intergenic
AACTTTAGCTGCAAGAATTA+AGG	0.311122	7.3:+52236493	None:intergenic
TTCAAACTTAGTTCTCTTTG+AGG	0.370756	7.3:+52235861	None:intergenic
TTTGCAGGAATTGAAACAAA+AGG	0.418042	7.3:-52235906	MS.gene23378:intron
TCAAAGAGAACTAAGTTTGA+AGG	0.442646	7.3:-52235859	None:intergenic
CTTGCAGCTAAAGTTATACA+AGG	0.476292	7.3:-52236485	MS.gene23378:intron
TTGCAGGAATTGAAACAAAA+GGG	0.500483	7.3:-52235905	MS.gene23378:intron
TAGTTCTCTTTGAGGTTGTG+AGG	0.552834	7.3:+52235869	None:intergenic
CAGGAATTGAAACAAAAGGG+TGG	0.635486	7.3:-52235902	MS.gene23378:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	GATTCTTCTTATTATTATAT+TGG	-	chr7.3:52236075-52236094	MS.gene23378:intron	15.0%
!!!	ATGTAATTTTGTAAAACTTA+TGG	+	chr7.3:52236273-52236292	None:intergenic	15.0%
!!!	TAAGTTTTACAAAATTACAT+TGG	-	chr7.3:52236273-52236292	MS.gene23378:intron	15.0%
!!	AAAAATACACCAATAAAGTA+AGG	+	chr7.3:52236199-52236218	None:intergenic	20.0%
!!	CATATTATACCTTACTTTAT+TGG	-	chr7.3:52236187-52236206	MS.gene23378:intron	20.0%
!!	TCTTTCAATTGTATATAGTT+TGG	-	chr7.3:52236103-52236122	MS.gene23378:intron	20.0%
!	AAATTAGTGCATGCTTAAAT+TGG	+	chr7.3:52236372-52236391	None:intergenic	25.0%
!	AAATTTGTAACATTCTTTGC+AGG	-	chr7.3:52236497-52236516	MS.gene23378:CDS	25.0%
!	AACAAAAAAAAAGAGGAGAA+TGG	-	chr7.3:52235863-52235882	MS.gene23378:CDS	25.0%
!	ACAAAAAAAAAGAGGAGAAT+GGG	-	chr7.3:52235864-52235883	MS.gene23378:CDS	25.0%
!	AGAATTCATTGGAACTTTAT+AGG	+	chr7.3:52236008-52236027	None:intergenic	25.0%
!	GAATCATAGTATCATTAAGT+AGG	+	chr7.3:52235978-52235997	None:intergenic	25.0%
!	GCAAAGAATGTTACAAATTT+AGG	+	chr7.3:52236497-52236516	None:intergenic	25.0%
!	TTAAAACTTCAACAAAATCG+TGG	+	chr7.3:52236301-52236320	None:intergenic	25.0%
!	TTGTAAAACTTATGGCAATT+TGG	+	chr7.3:52236265-52236284	None:intergenic	25.0%
!!	TGAAAAAAAGAAAGAGTGTA+AGG	+	chr7.3:52236046-52236065	None:intergenic	25.0%
!!!	TTTCTTTTTTTCACTTCTCT+TGG	-	chr7.3:52236053-52236072	MS.gene23378:intron	25.0%
	AACTTTAGCTGCAAGAATTA+AGG	+	chr7.3:52235928-52235947	None:intergenic	30.0%
	AGCACTTCTCTAAATTATCA+AGG	-	chr7.3:52236399-52236418	MS.gene23378:intron	30.0%
	TTGCAGGAATTGAAACAAAA+GGG	-	chr7.3:52236513-52236532	MS.gene23378:CDS	30.0%
	TTTGCAGGAATTGAAACAAA+AGG	-	chr7.3:52236512-52236531	MS.gene23378:CDS	30.0%
!	TTCAAACTTAGTTCTCTTTG+AGG	+	chr7.3:52236560-52236579	None:intergenic	30.0%
	AGAAGAAGGTGAGAATTCAT+TGG	+	chr7.3:52236019-52236038	None:intergenic	35.0%
	CTTGCAGCTAAAGTTATACA+AGG	-	chr7.3:52235933-52235952	MS.gene23378:intron	35.0%
	TTAGTGCATGCTTAAATTGG+TGG	+	chr7.3:52236369-52236388	None:intergenic	35.0%
!	AAGAGTAGCATGAGTACTTT+TGG	+	chr7.3:52235903-52235922	None:intergenic	35.0%
!	AGAGTAGCATGAGTACTTTT+GGG	+	chr7.3:52235902-52235921	None:intergenic	35.0%
	CAGGAATTGAAACAAAAGGG+TGG	-	chr7.3:52236516-52236535	MS.gene23378:CDS	40.0%
	GAGTGTAAGGACTAAGAAGA+AGG	+	chr7.3:52236033-52236052	None:intergenic	40.0%
!	TGCATGCTTAAATTGGTGGT+GGG	+	chr7.3:52236365-52236384	None:intergenic	40.0%
!!	CATGAGTACTTTTGGGAGTT+TGG	+	chr7.3:52235895-52235914	None:intergenic	40.0%
!!	TAGTTCTCTTTGAGGTTGTG+AGG	+	chr7.3:52236552-52236571	None:intergenic	40.0%
	GTGCATGCTTAAATTGGTGG+TGG	+	chr7.3:52236366-52236385	None:intergenic	45.0%

Chromosome	Type	Strat	End	Strand	Name
chr7.3	gene	52235860	52236580	52235860	ID=MS.gene23378
chr7.3	mRNA	52235860	52236580	52235860	ID=MS.gene23378.t1;Parent=MS.gene23378
chr7.3	exon	52236486	52236580	52236486	ID=MS.gene23378.t1.exon1;Parent=MS.gene23378.t1
chr7.3	CDS	52236486	52236580	52236486	ID=cds.MS.gene23378.t1;Parent=MS.gene23378.t1
chr7.3	exon	52235860	52235921	52235860	ID=MS.gene23378.t1.exon2;Parent=MS.gene23378.t1
chr7.3	CDS	52235860	52235921	52235860	ID=cds.MS.gene23378.t1;Parent=MS.gene23378.t1

Gene Sequence

>MS.gene23378

CATAACAAAAAAAAAGAGGAGAATGGGTAACACCAAACTCCCAAAAGTACTCATGCTACTCTTGACCTTAATTCTTGCAGCTAAAGTTATACAAGGTCTACTCATGTCATATTGTCCTACTTAATGATACTATGATTCTTCTTTTCCTATAAAGTTCCAATGAATTCTCACCTTCTTCTTAGTCCTTACACTCTTTCTTTTTTTCACTTCTCTTGGATTCTTCTTATTATTATATTGGATGCTTCTTTCAATTGTATATAGTTTGGAGCATATATTAACTGTATTCTTAACATAAACATATATAATACTCATAATATTAGTGTTATTCATATTATACCTTACTTTATTGGTGTATTTTTCTTTATGTTATATATGATTTGCTTTAGACATTAAGTGCATTTTCCAAATTGCCATAAGTTTTACAAAATTACATTGGTGCCACGATTTTGTTGAAGTTTTAAAATGTAATATTTGCAAAAAAAAAAAATGTAATTATTCTAAACCCACCACCAATTTAAGCATGCACTAATTTCAACAAAAGCACTTCTCTAAATTATCAAGGTTGTCAAAAAACACTTTGCAGAGAAAAATAATTTAGTCTCAATTTCTTGATGCATATGCATTTTCAATGTATCCTAAATTTGTAACATTCTTTGCAGGAATTGAAACAAAAGGGTGGATATCACAATCCTCACAACCTCAAAGAGAACTAAGTTTGAAG

Protein sequence

>MS.gene23378.t1

HNKKKEENGUHQTPKSTHATLDLNSCSUSYTRNUNKRVDITILTTSKRTKFE