AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene24735


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene24735.t1 MTR_7g104720 96.109 257 4 2 1 251 1 257 1.45e-175 489
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene24735.t1 AT5G03870 38.168 262 122 13 1 251 1 233 3.01e-27 108

Find 65 sgRNAs with CRISPR-Local

Find 77 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CTGTTCCTTCTTGCAGAAAA+TGG 0.194971 7.1:+6862843 None:intergenic
TTGAAGTTTCTTAGTCAATA+TGG 0.198310 7.1:-6863020 MS.gene24735:CDS
TTGATGTTGAAGACAGTTTC+TGG 0.216462 7.1:+6863161 None:intergenic
GGTGGTGATGGGTTTGATTT+GGG 0.220205 7.1:+6863194 None:intergenic
GGAGACCCTTTCAAATTCTT+AGG 0.290595 7.1:+6862864 None:intergenic
GGGTGGTGATGGGTTTGATT+TGG 0.301104 7.1:+6863193 None:intergenic
GGGTCTTTGTGGGGTGGTGA+TGG 0.322134 7.1:+6863182 None:intergenic
GGCTTCTTGAGTGTAGATTT+TGG 0.327883 7.1:+6862993 None:intergenic
TAAGGTTCAAGTTACTAATA+TGG 0.330515 7.1:-6862955 MS.gene24735:CDS
GCCATTGGGGAAAGAGAATA+AGG 0.342459 7.1:-6862973 MS.gene24735:CDS
GAAGAAAAGGTGCGGACTTT+GGG 0.352950 7.1:+6863075 None:intergenic
TCTGCAAGAAGGAACAGTTT+TGG 0.354149 7.1:-6862837 MS.gene24735:CDS
TTTCTTTGGAAAATTTGAAA+TGG 0.363338 7.1:+6863100 None:intergenic
CGAAGAAAAGGTGCGGACTT+TGG 0.372618 7.1:+6863074 None:intergenic
AAGAGGTGGTGTTAGACGTT+TGG 0.372997 7.1:-6862931 MS.gene24735:CDS
GGTCTTTGTGGGGTGGTGAT+GGG 0.382298 7.1:+6863183 None:intergenic
TGAAGTTTCTTAGTCAATAT+GGG 0.397473 7.1:-6863019 MS.gene24735:CDS
TTCTTTGGAAAATTTGAAAT+GGG 0.406127 7.1:+6863101 None:intergenic
GACAGTTTCTGGGTCTTTGT+GGG 0.416119 7.1:+6863172 None:intergenic
TCATAGGATGCAATAATCTC+TGG 0.418703 7.1:+6862762 None:intergenic
GACTCTGTTTCTGAAACAAT+GGG 0.424980 7.1:+6863227 None:intergenic
TCTACACTCAAGAAGCCATT+GGG 0.429305 7.1:-6862987 MS.gene24735:CDS
TGATGTTGAAGACAGTTTCT+GGG 0.432089 7.1:+6863162 None:intergenic
TGTTCTCAAACAAGTTCAAA+AGG 0.437472 7.1:+6862646 None:intergenic
TGACTCTGTTTCTGAAACAA+TGG 0.442742 7.1:+6863226 None:intergenic
GTAGATTTCAAAATTCCCTT+AGG 0.443534 7.1:+6862900 None:intergenic
TCTCTCACTTCCTCAACCTA+TGG 0.444832 7.1:-6863290 MS.gene24735:CDS
CTCATGGAGGGTCTTGAAGA+AGG 0.446915 7.1:-6863128 MS.gene24735:CDS
TGTTTCTGAAACAATGGGAT+TGG 0.446998 7.1:+6863232 None:intergenic
AGACAGTTTCTGGGTCTTTG+TGG 0.450080 7.1:+6863171 None:intergenic
GCCATAAACCCACGAAGAAA+AGG 0.461759 7.1:+6863062 None:intergenic
ACGTTTGGATTATAGTCCTA+AGG 0.462177 7.1:-6862916 MS.gene24735:CDS
CATCAATGCTTGGGAACTCA+TGG 0.467655 7.1:-6863144 MS.gene24735:CDS
CTGTCTTCAACATCAATGCT+TGG 0.469093 7.1:-6863154 MS.gene24735:CDS
TATTGACTAAGAAACTTCAA+TGG 0.475559 7.1:+6863023 None:intergenic
TCTTCTCCTAAGAATTTGAA+AGG 0.476501 7.1:-6862870 MS.gene24735:CDS
ACCTTATTCTCTTTCCCCAA+TGG 0.478528 7.1:+6862972 None:intergenic
GAAACAACATGGTTAATGTA+AGG 0.487432 7.1:+6863311 None:intergenic
TGTCTTCAACATCAATGCTT+GGG 0.495814 7.1:-6863153 MS.gene24735:CDS
GTTTAACTTGAGTGCTCCAT+AGG 0.496842 7.1:+6863274 None:intergenic
CTTGAGTGCTCCATAGGTTG+AGG 0.498615 7.1:+6863280 None:intergenic
GATTATTGCATCCTATGAAA+AGG 0.512501 7.1:-6862757 MS.gene24735:CDS
ATTCTTAGGAGAAGAATTTG+TGG 0.515792 7.1:+6862878 None:intergenic
CAAGTTACTAATATGGTAAG+AGG 0.516161 7.1:-6862948 MS.gene24735:CDS
GTTTCTGGGTCTTTGTGGGG+TGG 0.516445 7.1:+6863176 None:intergenic
CTTCTCCTAAGAATTTGAAA+GGG 0.521946 7.1:-6862869 MS.gene24735:CDS
CGTTTGGATTATAGTCCTAA+GGG 0.522025 7.1:-6862915 MS.gene24735:CDS
GTTCTCAAACAAGTTCAAAA+GGG 0.522310 7.1:+6862647 None:intergenic
ATCTACACTCAAGAAGCCAT+TGG 0.526227 7.1:-6862988 MS.gene24735:CDS
TCATGGAGGGTCTTGAAGAA+GGG 0.530353 7.1:-6863127 MS.gene24735:CDS
GTTTGAGAACAAGTGTCCTC+CGG 0.539932 7.1:-6862634 MS.gene24735:CDS
AAAGGACTAGGAACAGAACT+AGG 0.541269 7.1:+6862792 None:intergenic
ACAGTTTCTGGGTCTTTGTG+GGG 0.544916 7.1:+6863173 None:intergenic
TGAAAAGGAACTTTCTGAAG+AGG 0.568581 7.1:-6862742 MS.gene24735:CDS
CAATGCTTGGGAACTCATGG+AGG 0.592089 7.1:-6863141 MS.gene24735:CDS
CATGGAGGGTCTTGAAGAAG+GGG 0.593805 7.1:-6863126 MS.gene24735:CDS
TTTAGAGGAAACGCAACCCA+TGG 0.597592 7.1:+6863367 None:intergenic
TCTGGATCAAACAAAGGACT+AGG 0.611554 7.1:+6862780 None:intergenic
ATAATCTCTGGATCAAACAA+AGG 0.612151 7.1:+6862774 None:intergenic
TGAGAACAAGTGTCCTCCGG+AGG 0.642958 7.1:-6862631 MS.gene24735:CDS
GTTACTAATATGGTAAGAGG+TGG 0.643768 7.1:-6862945 MS.gene24735:CDS
AATGCTTGGGAACTCATGGA+GGG 0.651425 7.1:-6863140 MS.gene24735:CDS
CTACACTCAAGAAGCCATTG+GGG 0.669155 7.1:-6862986 MS.gene24735:CDS
AGGAAGTGAGAGAAACAACA+TGG 0.694964 7.1:+6863300 None:intergenic
AAACCCACGAAGAAAAGGTG+CGG 0.704843 7.1:+6863067 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TAATGTAAGGATTTTTTTCT+TGG + chr7.1:6862658-6862677 None:intergenic 20.0%
!!! TTCTTTGGAAAATTTGAAAT+GGG + chr7.1:6862881-6862900 None:intergenic 20.0%
!!! TTTCTTTGGAAAATTTGAAA+TGG + chr7.1:6862882-6862901 None:intergenic 20.0%
! TGAAGTTTCTTAGTCAATAT+GGG - chr7.1:6862960-6862979 MS.gene24735:CDS 25.0%
! TTGAAGTTTCTTAGTCAATA+TGG - chr7.1:6862959-6862978 MS.gene24735:CDS 25.0%
!! TAAGGTTCAAGTTACTAATA+TGG - chr7.1:6863024-6863043 MS.gene24735:CDS 25.0%
!! TATTGACTAAGAAACTTCAA+TGG + chr7.1:6862959-6862978 None:intergenic 25.0%
!!! TTCTTTTTTGATGTGTTTAG+AGG + chr7.1:6862630-6862649 None:intergenic 25.0%
ATAATCTCTGGATCAAACAA+AGG + chr7.1:6863208-6863227 None:intergenic 30.0%
ATTCTTAGGAGAAGAATTTG+TGG + chr7.1:6863104-6863123 None:intergenic 30.0%
CAAGTTACTAATATGGTAAG+AGG - chr7.1:6863031-6863050 MS.gene24735:CDS 30.0%
CTTCTCCTAAGAATTTGAAA+GGG - chr7.1:6863110-6863129 MS.gene24735:CDS 30.0%
GAAACAACATGGTTAATGTA+AGG + chr7.1:6862671-6862690 None:intergenic 30.0%
GATTATTGCATCCTATGAAA+AGG - chr7.1:6863222-6863241 MS.gene24735:CDS 30.0%
GTAGATTTCAAAATTCCCTT+AGG + chr7.1:6863082-6863101 None:intergenic 30.0%
GTTCTCAAACAAGTTCAAAA+GGG + chr7.1:6863335-6863354 None:intergenic 30.0%
TCTTCTCCTAAGAATTTGAA+AGG - chr7.1:6863109-6863128 MS.gene24735:CDS 30.0%
TGTTCTCAAACAAGTTCAAA+AGG + chr7.1:6863336-6863355 None:intergenic 30.0%
! TTCAGAAAGTTCCTTTTCAT+AGG + chr7.1:6863236-6863255 None:intergenic 30.0%
ACGTTTGGATTATAGTCCTA+AGG - chr7.1:6863063-6863082 MS.gene24735:CDS 35.0%
CGTTTGGATTATAGTCCTAA+GGG - chr7.1:6863064-6863083 MS.gene24735:CDS 35.0%
GTTACTAATATGGTAAGAGG+TGG - chr7.1:6863034-6863053 MS.gene24735:CDS 35.0%
TCATAGGATGCAATAATCTC+TGG + chr7.1:6863220-6863239 None:intergenic 35.0%
TGATGTTGAAGACAGTTTCT+GGG + chr7.1:6862820-6862839 None:intergenic 35.0%
TGTCTTCAACATCAATGCTT+GGG - chr7.1:6862826-6862845 MS.gene24735:CDS 35.0%
TTGATGTTGAAGACAGTTTC+TGG + chr7.1:6862821-6862840 None:intergenic 35.0%
! TACAACAGAATTTTCTCCTC+CGG + chr7.1:6863367-6863386 None:intergenic 35.0%
! TGAAAAGGAACTTTCTGAAG+AGG - chr7.1:6863237-6863256 MS.gene24735:CDS 35.0%
!! GACTCTGTTTCTGAAACAAT+GGG + chr7.1:6862755-6862774 None:intergenic 35.0%
!! TGACTCTGTTTCTGAAACAA+TGG + chr7.1:6862756-6862775 None:intergenic 35.0%
!! TGTTTCTGAAACAATGGGAT+TGG + chr7.1:6862750-6862769 None:intergenic 35.0%
AAAGGACTAGGAACAGAACT+AGG + chr7.1:6863190-6863209 None:intergenic 40.0%
ACCTTATTCTCTTTCCCCAA+TGG + chr7.1:6863010-6863029 None:intergenic 40.0%
AGGAAGTGAGAGAAACAACA+TGG + chr7.1:6862682-6862701 None:intergenic 40.0%
ATCTACACTCAAGAAGCCAT+TGG - chr7.1:6862991-6863010 MS.gene24735:CDS 40.0%
CTGTCTTCAACATCAATGCT+TGG - chr7.1:6862825-6862844 MS.gene24735:CDS 40.0%
CTGTTCCTTCTTGCAGAAAA+TGG + chr7.1:6863139-6863158 None:intergenic 40.0%
GGAGACCCTTTCAAATTCTT+AGG + chr7.1:6863118-6863137 None:intergenic 40.0%
TCTACACTCAAGAAGCCATT+GGG - chr7.1:6862992-6863011 MS.gene24735:CDS 40.0%
TCTGGATCAAACAAAGGACT+AGG + chr7.1:6863202-6863221 None:intergenic 40.0%
! ACCTTTTCTTCGTGGGTTTA+TGG - chr7.1:6862916-6862935 MS.gene24735:CDS 40.0%
! CTGCAAGAAGGAACAGTTTT+GGG - chr7.1:6863143-6863162 MS.gene24735:CDS 40.0%
! GGCTTCTTGAGTGTAGATTT+TGG + chr7.1:6862989-6863008 None:intergenic 40.0%
! TCTGCAAGAAGGAACAGTTT+TGG - chr7.1:6863142-6863161 MS.gene24735:CDS 40.0%
! TTTCTCGCTCTTCGTGTTTT+GGG + chr7.1:6863295-6863314 None:intergenic 40.0%
! TTTTCTCGCTCTTCGTGTTT+TGG + chr7.1:6863296-6863315 None:intergenic 40.0%
!! GTTTAACTTGAGTGCTCCAT+AGG + chr7.1:6862708-6862727 None:intergenic 40.0%
AAACCCACGAAGAAAAGGTG+CGG + chr7.1:6862915-6862934 None:intergenic 45.0%
AATGCTTGGGAACTCATGGA+GGG - chr7.1:6862839-6862858 MS.gene24735:CDS 45.0%
CATCAATGCTTGGGAACTCA+TGG - chr7.1:6862835-6862854 MS.gene24735:CDS 45.0%
CTACACTCAAGAAGCCATTG+GGG - chr7.1:6862993-6863012 MS.gene24735:CDS 45.0%
GCCATAAACCCACGAAGAAA+AGG + chr7.1:6862920-6862939 None:intergenic 45.0%
GCCATTGGGGAAAGAGAATA+AGG - chr7.1:6863006-6863025 MS.gene24735:CDS 45.0%
GTTTGAGAACAAGTGTCCTC+CGG - chr7.1:6863345-6863364 MS.gene24735:CDS 45.0%
TCATGGAGGGTCTTGAAGAA+GGG - chr7.1:6862852-6862871 MS.gene24735:CDS 45.0%
TCTCTCACTTCCTCAACCTA+TGG - chr7.1:6862689-6862708 MS.gene24735:CDS 45.0%
! AACAGAATTTTCTCCTCCGG+AGG + chr7.1:6863364-6863383 None:intergenic 45.0%
! AAGAGGTGGTGTTAGACGTT+TGG - chr7.1:6863048-6863067 MS.gene24735:CDS 45.0%
! ACAGTTTCTGGGTCTTTGTG+GGG + chr7.1:6862809-6862828 None:intergenic 45.0%
! AGACAGTTTCTGGGTCTTTG+TGG + chr7.1:6862811-6862830 None:intergenic 45.0%
! GAAGAAAAGGTGCGGACTTT+GGG + chr7.1:6862907-6862926 None:intergenic 45.0%
! GACAGTTTCTGGGTCTTTGT+GGG + chr7.1:6862810-6862829 None:intergenic 45.0%
! GGTCTCCATTTTCTGCAAGA+AGG - chr7.1:6863131-6863150 MS.gene24735:CDS 45.0%
!! GGTGGTGATGGGTTTGATTT+GGG + chr7.1:6862788-6862807 None:intergenic 45.0%
CAATGCTTGGGAACTCATGG+AGG - chr7.1:6862838-6862857 MS.gene24735:CDS 50.0%
CATGGAGGGTCTTGAAGAAG+GGG - chr7.1:6862853-6862872 MS.gene24735:CDS 50.0%
CTCATGGAGGGTCTTGAAGA+AGG - chr7.1:6862851-6862870 MS.gene24735:CDS 50.0%
CTTGAGTGCTCCATAGGTTG+AGG + chr7.1:6862702-6862721 None:intergenic 50.0%
! AAGTCCGCACCTTTTCTTCG+TGG - chr7.1:6862908-6862927 MS.gene24735:CDS 50.0%
! AGTCCGCACCTTTTCTTCGT+GGG - chr7.1:6862909-6862928 MS.gene24735:CDS 50.0%
! CGAAGAAAAGGTGCGGACTT+TGG + chr7.1:6862908-6862927 None:intergenic 50.0%
! GCGGACTTTGGGCTTTTCTT+TGG + chr7.1:6862896-6862915 None:intergenic 50.0%
!! GGGTGGTGATGGGTTTGATT+TGG + chr7.1:6862789-6862808 None:intergenic 50.0%
GGTCTTTGTGGGGTGGTGAT+GGG + chr7.1:6862799-6862818 None:intergenic 55.0%
TGAGAACAAGTGTCCTCCGG+AGG - chr7.1:6863348-6863367 MS.gene24735:CDS 55.0%
! GTTTCTGGGTCTTTGTGGGG+TGG + chr7.1:6862806-6862825 None:intergenic 55.0%
GGGTCTTTGTGGGGTGGTGA+TGG + chr7.1:6862800-6862819 None:intergenic 60.0%


Chromosome Type Strat End Strand Name
chr7.1 gene 6862614 6863387 6862614 ID=MS.gene24735
chr7.1 mRNA 6862614 6863387 6862614 ID=MS.gene24735.t1;Parent=MS.gene24735
chr7.1 exon 6862614 6863387 6862614 ID=MS.gene24735.t1.exon1;Parent=MS.gene24735.t1
chr7.1 CDS 6862614 6863387 6862614 ID=cds.MS.gene24735.t1;Parent=MS.gene24735.t1
Gene Sequence

>MS.gene24735

ATGGGTTGCGTTTCCTCTAAACACATCAAAAAAGAAATCAACCAAGAAAAAAATCCTTACATTAACCATGTTGTTTCTCTCACTTCCTCAACCTATGGAGCACTCAAGTTAAACAACAACAACAACGAATCATCCAATCCCATTGTTTCAGAAACAGAGTCAAAAACAGAACCCAAATCAAACCCATCACCACCCCACAAAGACCCAGAAACTGTCTTCAACATCAATGCTTGGGAACTCATGGAGGGTCTTGAAGAAGGGGTGCCCATTTCAAATTTTCCAAAGAAAAGCCCAAAGTCCGCACCTTTTCTTCGTGGGTTTATGGCTTCTGACACAAGAAGTCCATTGAAGTTTCTTAGTCAATATGGGTCACCAAAATCTACACTCAAGAAGCCATTGGGGAAAGAGAATAAGGTTCAAGTTACTAATATGGTAAGAGGTGGTGTTAGACGTTTGGATTATAGTCCTAAGGGAATTTTGAAATCTACCACAAATTCTTCTCCTAAGAATTTGAAAGGGTCTCCATTTTCTGCAAGAAGGAACAGTTTTGGGAATGAGAGTGTGAGAAAAAGTCCTAGTTCTGTTCCTAGTCCTTTGTTTGATCCAGAGATTATTGCATCCTATGAAAAGGAACTTTCTGAAGAGGAAGAACAAATCAAGAGAATTGTTTTTGCAACACCCAAAACACGAAGAGCGAGAAAATCTCTTGATTCAATTGCCCTTTTGAACTTGTTTGAGAACAAGTGTCCTCCGGAGGAGAAAATTCTGTTGTAA

Protein sequence

>MS.gene24735.t1

MGCVSSKHIKKEINQEKNPYINHVVSLTSSTYGALKLNNNNNESSNPIVSETESKTEPKSNPSPPHKDPETVFNINAWELMEGLEEGVPISNFPKKSPKSAPFLRGFMASDTRSPLKFLSQYGSPKSTLKKPLGKENKVQVTNMVRGGVRRLDYSPKGILKSTTNSSPKNLKGSPFSARRNSFGNESVRKSPSSVPSPLFDPEIIASYEKELSEEEEQIKRIVFATPKTRRARKSLDSIALLNLFENKCPPEEKILL