AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene25178

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene25178.t1 MTR_1g032450 99.639 277 1 0 1 277 1 277 0.0 558
MS.gene25178.t1 MTR_8g105340 94.662 281 9 3 1 277 1 279 0.0 524
MS.gene25178.t1 MTR_8g105340 98.729 236 3 0 42 277 35 270 5.24e-173 478
MS.gene25178.t1 MTR_4g088615 32.727 165 94 4 89 244 147 303 2.36e-15 75.1
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene25178.t1 AT3G57490 92.308 221 17 0 43 263 41 261 2.31e-152 426
MS.gene25178.t1 AT2G41840 90.308 227 19 1 42 268 49 272 4.66e-151 424
MS.gene25178.t1 AT1G58684 87.712 236 26 1 43 275 49 284 2.68e-150 422
MS.gene25178.t1 AT1G58983 87.712 236 26 1 43 275 49 284 2.68e-150 422
MS.gene25178.t1 AT1G59359 87.712 236 26 1 43 275 49 284 2.68e-150 422
MS.gene25178.t1 AT1G58380 87.342 237 27 1 42 275 48 284 3.38e-150 421
MS.gene25178.t1 AT2G33800 29.530 149 97 2 89 237 147 287 2.33e-14 72.0

Find 92 sgRNAs with CRISPR-Local

Find 165 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GTTTCTTGACACCTGAATTC+TGG 0.269557 1.3:+17388696 MS.gene25178:CDS
TCCTGTGAGAAGAGGGTATT+GGG 0.277304 1.3:+17386983 MS.gene25178:CDS
GCAAACTGCAAAACCTTCTT+AGG 0.278482 1.3:-17387122 None:intergenic
AACGAAATTGCCAAGGGTTT+TGG 0.290170 1.3:-17387183 None:intergenic
GATGATTTGGTGTTCTTTGA+TGG 0.310931 1.3:-17386754 None:intergenic
GACTGTCTGATGAAGACTTA+TGG 0.329475 1.3:+17388674 MS.gene25178:CDS
GTTCCTGCTCCTCGTGGTTC+TGG 0.333939 1.3:+17387080 MS.gene25178:CDS
CGAGTCTCCTTCCAGAATTC+AGG 0.345000 1.3:-17388707 None:intergenic
GCCGGATTCGGACGTGGTTT+CGG 0.349482 1.3:+17386564 MS.gene25178:CDS
CGACTAAGGTGTCGATGATT+TGG 0.372123 1.3:-17386767 None:intergenic
CCAACATATCAGTGTACTCT+TGG 0.379970 1.3:-17388748 None:intergenic
CACAACAAAGGCCTTGATCT+TGG 0.385331 1.3:+17388778 MS.gene25178:CDS
CCGGGTTCGTTGACCAGCTC+GGG 0.393708 1.3:-17386841 None:intergenic
CAACTACAAAAGCTTTGAAC+CGG 0.394296 1.3:-17386860 None:intergenic
ACTGTGCCGTGTAAGGTTAC+TGG 0.397057 1.3:+17387029 MS.gene25178:CDS
AAGATCAAGGCCTTTGTTGT+GGG 0.399949 1.3:-17388776 None:intergenic
AACCCTTGGCAATTTCGTTA+AGG 0.410713 1.3:+17387187 MS.gene25178:CDS
TTCTGGTATTGTTGCTGCTA+GGG 0.416284 1.3:+17387097 MS.gene25178:CDS
CAAGATCAAGGCCTTTGTTG+TGG 0.418524 1.3:-17388777 None:intergenic
TGCTGCTAGGGTTCCTAAGA+AGG 0.419749 1.3:+17387109 MS.gene25178:CDS
TCCGAAACCACGTCCGAATC+CGG 0.425394 1.3:-17386565 None:intergenic
GCTCGGGTTTGTTTCTGAAC+CGG 0.426277 1.3:-17386825 None:intergenic
GTTCTTTGATGGGGAGAGAG+TGG 0.429180 1.3:-17386743 None:intergenic
GGAGGTCGCCGCAGAGGCGC+TGG 0.430160 1.3:+17386627 MS.gene25178:CDS
GTTAGGTGTGAAGTGTAGTA+AGG 0.432604 1.3:+17386908 MS.gene25178:CDS
CTCTGTCTCCGGTAGCTCCT+CGG 0.448842 1.3:-17386542 None:intergenic
TCCGAATCCGGCTCTGTCTC+CGG 0.455885 1.3:-17386553 None:intergenic
ACCGGAGACAGAGCCGGATT+CGG 0.457200 1.3:+17386552 MS.gene25178:CDS
TTTCAAAGTGGGACCGACTA+AGG 0.461437 1.3:-17386781 None:intergenic
ATGATTTGGTGTTCTTTGAT+GGG 0.466975 1.3:-17386753 None:intergenic
CTTGATCTTGGAGGAGGAAA+GGG 0.473493 1.3:+17388790 MS.gene25178:CDS
ACCGGGTTCGTTGACCAGCT+CGG 0.475496 1.3:-17386842 None:intergenic
CTCGGCCGTCTCGTGAAAGA+AGG 0.476551 1.3:+17386690 MS.gene25178:CDS
TTCCTGTGAGAAGAGGGTAT+TGG 0.480661 1.3:+17386982 MS.gene25178:CDS
GTTCTGGTATTGTTGCTGCT+AGG 0.486256 1.3:+17387096 MS.gene25178:CDS
ACCACATACTGTGCCGTGTA+AGG 0.490382 1.3:+17387022 MS.gene25178:CDS
GGGTATTGGGGAAATAAGAT+TGG 0.503409 1.3:+17386996 MS.gene25178:CDS
CCCCAATACCCTCTTCTCAC+AGG 0.507999 1.3:-17386984 None:intergenic
ACCTTACACGGCACAGTATG+TGG 0.514029 1.3:-17387023 None:intergenic
AACAATGGTCATGTAGGGTT+AGG 0.515658 1.3:+17386891 MS.gene25178:CDS
ATCACTTCGTCTTTCAAAGT+GGG 0.517922 1.3:-17386792 None:intergenic
CCTTTCCTCCTCCAAGATCA+AGG 0.523177 1.3:-17388789 None:intergenic
ATACCAGAACCACGAGGAGC+AGG 0.524892 1.3:-17387083 None:intergenic
AGAGGCGGAGATCGTGGCCG+TGG 0.527803 1.3:+17386606 MS.gene25178:CDS
TGGTTCTGTTACTGTTCGTA+TGG 0.533362 1.3:+17387058 MS.gene25178:CDS
CCAAGAGTACACTGATATGT+TGG 0.536690 1.3:+17388748 MS.gene25178:CDS
CCCGAGCTGGTCAACGAACC+CGG 0.537918 1.3:+17386841 MS.gene25178:CDS
CGTGGTCGAGGTGGCAGAGG+CGG 0.538891 1.3:+17386591 MS.gene25178:CDS
CACTTTCCAGTAACCTTACA+CGG 0.538940 1.3:-17387035 None:intergenic
CATCACTTCGTCTTTCAAAG+TGG 0.540142 1.3:-17386793 None:intergenic
ATGGCAGACCGAGGAGCTAC+CGG 0.543216 1.3:+17386534 MS.gene25178:CDS
AGGCGCTGGCCGTCGTGAAG+AGG 0.545371 1.3:+17386641 MS.gene25178:CDS
GGTATTGGGGAAATAAGATT+GGG 0.546642 1.3:+17386997 MS.gene25178:CDS
CGCCTCTGCGGCGACCTCCA+CGG 0.546686 1.3:-17386623 None:intergenic
GACAGAGCCGGATTCGGACG+TGG 0.547021 1.3:+17386558 MS.gene25178:CDS
CTTGACACCTGAATTCTGGA+AGG 0.547375 1.3:+17388700 MS.gene25178:CDS
CCTTGATCTTGGAGGAGGAA+AGG 0.547470 1.3:+17388789 MS.gene25178:CDS
AACAAAGGCCTTGATCTTGG+AGG 0.548080 1.3:+17388781 MS.gene25178:CDS
CTTTGATGGGGAGAGAGTGG+AGG 0.552588 1.3:-17386740 None:intergenic
GATCGTGGTCGAGGTGGCAG+AGG 0.555874 1.3:+17386588 MS.gene25178:CDS
ATATCAGTGTACTCTTGGAA+TGG 0.556129 1.3:-17388743 None:intergenic
AGGGGATCTACCAAAACCCT+TGG 0.565634 1.3:+17387173 MS.gene25178:CDS
GGTTTCGGAGATCGTGGTCG+AGG 0.566071 1.3:+17386579 MS.gene25178:CDS
GGAGCTACCGGAGACAGAGC+CGG 0.566711 1.3:+17386546 MS.gene25178:CDS
AAAGGCCTTGATCTTGGAGG+AGG 0.570185 1.3:+17388784 MS.gene25178:CDS
GAAGTTGCTACTGCAATTCG+CGG 0.571598 1.3:+17386930 MS.gene25178:CDS
GTTCAGAAACAAACCCGAGC+TGG 0.581420 1.3:+17386828 MS.gene25178:CDS
GGTGGCAGAGGCGGAGATCG+TGG 0.589603 1.3:+17386600 MS.gene25178:CDS
AACTACAAAAGCTTTGAACC+GGG 0.591946 1.3:-17386859 None:intergenic
GGCCGTGGAGGTCGCCGCAG+AGG 0.598782 1.3:+17386621 MS.gene25178:CDS
GGTGACAACAATGGTCATGT+AGG 0.601531 1.3:+17386885 MS.gene25178:CDS
TGATTTGGTGTTCTTTGATG+GGG 0.602663 1.3:-17386752 None:intergenic
AGACCTTAACGAAATTGCCA+AGG 0.604222 1.3:-17387190 None:intergenic
CGAAGTGATGAAAATCATGC+CGG 0.605193 1.3:+17386806 MS.gene25178:CDS
GGACGTGGTTTCGGAGATCG+TGG 0.605544 1.3:+17386573 MS.gene25178:CDS
GTTGGCAAAGCCCACAACAA+AGG 0.608366 1.3:+17388766 MS.gene25178:CDS
GTGACAACAATGGTCATGTA+GGG 0.608986 1.3:+17386886 MS.gene25178:CDS
GACCTTAACGAAATTGCCAA+GGG 0.610712 1.3:-17387189 None:intergenic
CACGACGGCCAGCGCCTCTG+CGG 0.615990 1.3:-17386635 None:intergenic
CGTATGGTTCCTGCTCCTCG+TGG 0.616774 1.3:+17387074 MS.gene25178:CDS
TGGGTCCCAGTAACAAAACT+CGG 0.617058 1.3:+17386672 MS.gene25178:CDS
TTCGGAGATCGTGGTCGAGG+TGG 0.624389 1.3:+17386582 MS.gene25178:CDS
TGTAAGGTTACTGGAAAGTG+TGG 0.643088 1.3:+17387038 MS.gene25178:CDS
CAAATCATCGACACCTTAGT+CGG 0.654265 1.3:+17386768 MS.gene25178:CDS
GTGTGATTCCTGTGAGAAGA+GGG 0.654445 1.3:+17386976 MS.gene25178:CDS
GGCGGAGATCGTGGCCGTGG+AGG 0.667002 1.3:+17386609 MS.gene25178:CDS
GATCTTGGAGGAGGAAAGGG+CGG 0.671501 1.3:+17388793 MS.gene25178:CDS
GTAGTTGTGGGTGACAACAA+TGG 0.680113 1.3:+17386876 MS.gene25178:CDS
CAACCAACAATGGCAGACCG+AGG 0.687866 1.3:+17386525 None:intergenic
AGTGTGATTCCTGTGAGAAG+AGG 0.697737 1.3:+17386975 MS.gene25178:CDS
CCTGTGAGAAGAGGGTATTG+GGG 0.718027 1.3:+17386984 MS.gene25178:CDS
GCAACAATACCAGAACCACG+AGG 0.777326 1.3:-17387089 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AATAAATGAATATATAAAAC+AGG - chr1.3:17387402-17387421 None:intergenic 10.0%
!! TTAATTGTATATTATAAACA+GGG + chr1.3:17388353-17388372 MS.gene25178:intron 10.0%
!! TTTAATTGTATATTATAAAC+AGG + chr1.3:17388352-17388371 MS.gene25178:intron 10.0%
!!! CTTTATTTCAATATTTTTTT+TGG + chr1.3:17388077-17388096 MS.gene25178:intron 10.0%
!!! ATTGATTTGAAAATAAATGA+TGG + chr1.3:17388054-17388073 MS.gene25178:intron 15.0%
!!! TATTTCAATATTTTTTTTGG+AGG + chr1.3:17388080-17388099 MS.gene25178:intron 15.0%
!!! TGTTTATGATTTTATGTTTA+CGG + chr1.3:17387260-17387279 MS.gene25178:intron 15.0%
!! AATTAATCATTTGCAAAACT+TGG + chr1.3:17387693-17387712 MS.gene25178:intron 20.0%
!! AATTTCAAAATATCATTCCA+AGG - chr1.3:17388380-17388399 None:intergenic 20.0%
!! ATTTCAAAATATCATTCCAA+GGG - chr1.3:17388379-17388398 None:intergenic 20.0%
!! CAACTAATAAAGTTTATATG+CGG - chr1.3:17388474-17388493 None:intergenic 20.0%
!! CATTTGTTTGTATAATCAAA+TGG + chr1.3:17388431-17388450 MS.gene25178:intron 20.0%
!! TCTTATTGTGAGTAAAATTT+AGG + chr1.3:17388009-17388028 MS.gene25178:intron 20.0%
!!! AATCCATTTAAAATGTAAAG+TGG - chr1.3:17388190-17388209 None:intergenic 20.0%
!!! ATCCATTTAAAATGTAAAGT+GGG - chr1.3:17388189-17388208 None:intergenic 20.0%
!!! ATCTTTAATTTGTTCATCAA+TGG + chr1.3:17388155-17388174 MS.gene25178:intron 20.0%
!!! TCATAGAATTTAACTTGAAA+TGG + chr1.3:17388232-17388251 MS.gene25178:intron 20.0%
!!! TTAATGCTATTTATTTTTGC+AGG + chr1.3:17388643-17388662 MS.gene25178:intron 20.0%
! AATAGATAGATAGAAACAAG+TGG - chr1.3:17387865-17387884 None:intergenic 25.0%
! ATTATAATCAACAGACTTGT+TGG + chr1.3:17388289-17388308 MS.gene25178:intron 25.0%
! GTAAATAGAAGCTAGATAAA+TGG + chr1.3:17387900-17387919 MS.gene25178:intron 25.0%
! TTTAATGTTCTTAACCACAA+GGG - chr1.3:17388407-17388426 None:intergenic 25.0%
!! TACCCACTTTACATTTTAAA+TGG + chr1.3:17388184-17388203 MS.gene25178:intron 25.0%
!! TTATAATCAACAGACTTGTT+GGG + chr1.3:17388290-17388309 MS.gene25178:intron 25.0%
!! TTTTAATGTTCTTAACCACA+AGG - chr1.3:17388408-17388427 None:intergenic 25.0%
!!! ATGATGTTTTTACTTCTTCA+AGG + chr1.3:17387153-17387172 MS.gene25178:CDS 25.0%
!!! TGATGTTTTTACTTCTTCAA+GGG + chr1.3:17387154-17387173 MS.gene25178:CDS 25.0%
!!! TTCAATATTTTTTTTGGAGG+AGG + chr1.3:17388083-17388102 MS.gene25178:intron 25.0%
AAAATAGCGAAGTCACTAAT+GGG + chr1.3:17387827-17387846 MS.gene25178:intron 30.0%
ATCTATCTATTTCCCTTGAA+TGG + chr1.3:17387874-17387893 MS.gene25178:intron 30.0%
CTATCAATTTACGAGATAGA+TGG + chr1.3:17387775-17387794 MS.gene25178:intron 30.0%
GATAAGTTGTTTATCCAAAC+AGG - chr1.3:17387443-17387462 None:intergenic 30.0%
GCTGGATATTTCAATATGAT+TGG + chr1.3:17388105-17388124 MS.gene25178:intron 30.0%
TAAAATAGCGAAGTCACTAA+TGG + chr1.3:17387826-17387845 MS.gene25178:intron 30.0%
TATGTGTATACAAACTTGAG+TGG + chr1.3:17387982-17388001 MS.gene25178:intron 30.0%
TCTATTTACTGTCCATTCAA+GGG - chr1.3:17387889-17387908 None:intergenic 30.0%
TTCTATTTACTGTCCATTCA+AGG - chr1.3:17387890-17387909 None:intergenic 30.0%
TTGTATAATGTGAACCTGTT+TGG + chr1.3:17387426-17387445 MS.gene25178:intron 30.0%
TTTCCATAAGTTCTCCAATA+TGG - chr1.3:17387521-17387540 None:intergenic 30.0%
! TCTGTTTTCATAAGCCATAT+TGG + chr1.3:17387504-17387523 MS.gene25178:intron 30.0%
! TTCAAAGCTTTTGTAGTTGT+GGG + chr1.3:17386864-17386883 MS.gene25178:CDS 30.0%
!! ATGATTTGGTGTTCTTTGAT+GGG - chr1.3:17386756-17386775 None:intergenic 30.0%
!! GATGTTTTTACTTCTTCAAG+GGG + chr1.3:17387155-17387174 MS.gene25178:CDS 30.0%
!! GTTTCATTGGTTTATAGTTC+TGG + chr1.3:17388537-17388556 MS.gene25178:intron 30.0%
!! TAGTGACTTCGCTATTTTAA+AGG - chr1.3:17387825-17387844 None:intergenic 30.0%
!! TTTGTGTTGGTTTGTTTCAT+TGG + chr1.3:17388524-17388543 MS.gene25178:intron 30.0%
AAGCCATATTGGAGAACTTA+TGG + chr1.3:17387515-17387534 MS.gene25178:intron 35.0%
AGAAACAAGTGGAAGATTAG+AGG - chr1.3:17387854-17387873 None:intergenic 35.0%
ATATCAGTGTACTCTTGGAA+TGG - chr1.3:17388746-17388765 None:intergenic 35.0%
ATCACTTCGTCTTTCAAAGT+GGG - chr1.3:17386795-17386814 None:intergenic 35.0%
CAAACACGAGTAACAAATTC+TGG - chr1.3:17388595-17388614 None:intergenic 35.0%
CATGCTTTCAAATGAAGTGT+TGG + chr1.3:17387945-17387964 MS.gene25178:intron 35.0%
GACATAAGTGATAGGTTAGT+GGG + chr1.3:17387797-17387816 MS.gene25178:intron 35.0%
GATAGATGGACATAAGTGAT+AGG + chr1.3:17387789-17387808 MS.gene25178:intron 35.0%
TATATTATAAACAGGGCCCT+TGG + chr1.3:17388360-17388379 MS.gene25178:intron 35.0%
TCGTGAAGAGGAAGAAAAAT+GGG + chr1.3:17386653-17386672 MS.gene25178:CDS 35.0%
TGGTGTGATTATATGTAGCT+AGG + chr1.3:17387713-17387732 MS.gene25178:intron 35.0%
! ATTTTTCTTCCTCTTCACGA+CGG - chr1.3:17386653-17386672 None:intergenic 35.0%
! CTTCATTTGAAAGCATGTCT+AGG - chr1.3:17387942-17387961 None:intergenic 35.0%
! GGTATTGGGGAAATAAGATT+GGG + chr1.3:17386997-17387016 MS.gene25178:CDS 35.0%
! GTTCAAAGCTTTTGTAGTTG+TGG + chr1.3:17386863-17386882 MS.gene25178:CDS 35.0%
!! AACTACAAAAGCTTTGAACC+GGG - chr1.3:17386862-17386881 None:intergenic 35.0%
!! ATGAAGTGTTGGTGCTAAAT+AGG + chr1.3:17387956-17387975 MS.gene25178:intron 35.0%
!! CAACTACAAAAGCTTTGAAC+CGG - chr1.3:17386863-17386882 None:intergenic 35.0%
!! GATGATTTGGTGTTCTTTGA+TGG - chr1.3:17386757-17386776 None:intergenic 35.0%
!! TGATTTGGTGTTCTTTGATG+GGG - chr1.3:17386755-17386774 None:intergenic 35.0%
!!! ATATTTTTTTTGGAGGAGGC+TGG + chr1.3:17388087-17388106 MS.gene25178:intron 35.0%
!!! TTTTGAAATTGACTCCCTTG+TGG + chr1.3:17388390-17388409 MS.gene25178:intron 35.0%
AACAATGGTCATGTAGGGTT+AGG + chr1.3:17386891-17386910 MS.gene25178:CDS 40.0%
AACCCTTGGCAATTTCGTTA+AGG + chr1.3:17387187-17387206 MS.gene25178:CDS 40.0%
AGACCTTAACGAAATTGCCA+AGG - chr1.3:17387193-17387212 None:intergenic 40.0%
CAAATCATCGACACCTTAGT+CGG + chr1.3:17386768-17386787 MS.gene25178:CDS 40.0%
CACTTTCCAGTAACCTTACA+CGG - chr1.3:17387038-17387057 None:intergenic 40.0%
CATACAGGGTCTATTGTGAT+TGG - chr1.3:17387643-17387662 None:intergenic 40.0%
CATCACTTCGTCTTTCAAAG+TGG - chr1.3:17386796-17386815 None:intergenic 40.0%
CCAACATATCAGTGTACTCT+TGG - chr1.3:17388751-17388770 None:intergenic 40.0%
CGAAGTGATGAAAATCATGC+CGG + chr1.3:17386806-17386825 MS.gene25178:CDS 40.0%
GACCTTAACGAAATTGCCAA+GGG - chr1.3:17387192-17387211 None:intergenic 40.0%
GACTGTCTGATGAAGACTTA+TGG + chr1.3:17388674-17388693 MS.gene25178:CDS 40.0%
GCAAACTGCAAAACCTTCTT+AGG - chr1.3:17387125-17387144 None:intergenic 40.0%
GCATACATAGATTCGATTGC+AGG - chr1.3:17388573-17388592 None:intergenic 40.0%
GGACATAAGTGATAGGTTAG+TGG + chr1.3:17387796-17387815 MS.gene25178:intron 40.0%
GGTTAGTGACAGTGTTAGTT+TGG + chr1.3:17387734-17387753 MS.gene25178:intron 40.0%
GTCATATATTGAGACAGTGC+TGG + chr1.3:17388493-17388512 MS.gene25178:intron 40.0%
GTCGTGAAGAGGAAGAAAAA+TGG + chr1.3:17386652-17386671 MS.gene25178:CDS 40.0%
GTGACAACAATGGTCATGTA+GGG + chr1.3:17386886-17386905 MS.gene25178:CDS 40.0%
GTTAGGTGTGAAGTGTAGTA+AGG + chr1.3:17386908-17386927 MS.gene25178:CDS 40.0%
GTTTCTTGACACCTGAATTC+TGG + chr1.3:17388696-17388715 MS.gene25178:CDS 40.0%
TGTAAGGTTACTGGAAAGTG+TGG + chr1.3:17387038-17387057 MS.gene25178:CDS 40.0%
TTCTGGTATTGTTGCTGCTA+GGG + chr1.3:17387097-17387116 MS.gene25178:CDS 40.0%
! AACGAAATTGCCAAGGGTTT+TGG - chr1.3:17387186-17387205 None:intergenic 40.0%
! AAGAAGGTTTTGCAGTTTGC+TGG + chr1.3:17387125-17387144 MS.gene25178:CDS 40.0%
! AAGATCAAGGCCTTTGTTGT+GGG - chr1.3:17388779-17388798 None:intergenic 40.0%
! CCAAGAGTACACTGATATGT+TGG + chr1.3:17388748-17388767 MS.gene25178:CDS 40.0%
! TGGTTCTGTTACTGTTCGTA+TGG + chr1.3:17387058-17387077 MS.gene25178:CDS 40.0%
! TTTTGCCTTCTTTCACGAGA+CGG - chr1.3:17386698-17386717 None:intergenic 40.0%
!! GGGTATTGGGGAAATAAGAT+TGG + chr1.3:17386996-17387015 MS.gene25178:CDS 40.0%
AACAAAGGCCTTGATCTTGG+AGG + chr1.3:17388781-17388800 MS.gene25178:CDS 45.0%
AGTGTGATTCCTGTGAGAAG+AGG + chr1.3:17386975-17386994 MS.gene25178:CDS 45.0%
CAAGATCAAGGCCTTTGTTG+TGG - chr1.3:17388780-17388799 None:intergenic 45.0%
CACAACAAAGGCCTTGATCT+TGG + chr1.3:17388778-17388797 MS.gene25178:CDS 45.0%
CTTGACACCTGAATTCTGGA+AGG + chr1.3:17388700-17388719 MS.gene25178:CDS 45.0%
CTTGATCTTGGAGGAGGAAA+GGG + chr1.3:17388790-17388809 MS.gene25178:CDS 45.0%
GAAGTTGCTACTGCAATTCG+CGG + chr1.3:17386930-17386949 MS.gene25178:CDS 45.0%
GGTGACAACAATGGTCATGT+AGG + chr1.3:17386885-17386904 MS.gene25178:CDS 45.0%
GTAGTTGTGGGTGACAACAA+TGG + chr1.3:17386876-17386895 MS.gene25178:CDS 45.0%
GTGTGATTCCTGTGAGAAGA+GGG + chr1.3:17386976-17386995 MS.gene25178:CDS 45.0%
GTTCTGGTATTGTTGCTGCT+AGG + chr1.3:17387096-17387115 MS.gene25178:CDS 45.0%
TGGGTCCCAGTAACAAAACT+CGG + chr1.3:17386672-17386691 MS.gene25178:CDS 45.0%
TTCGCGCTTCTTAGCATACA+GGG - chr1.3:17387657-17387676 None:intergenic 45.0%
TTTCAAAGTGGGACCGACTA+AGG - chr1.3:17386784-17386803 None:intergenic 45.0%
! GCTGGATGAGTAGTTTGTGT+TGG + chr1.3:17388511-17388530 MS.gene25178:intron 45.0%
! TTCCTGTGAGAAGAGGGTAT+TGG + chr1.3:17386982-17387001 MS.gene25178:CDS 45.0%
!! CGACTAAGGTGTCGATGATT+TGG - chr1.3:17386770-17386789 None:intergenic 45.0%
!! TCCTGTGAGAAGAGGGTATT+GGG + chr1.3:17386983-17387002 MS.gene25178:CDS 45.0%
AAAGGCCTTGATCTTGGAGG+AGG + chr1.3:17388784-17388803 MS.gene25178:CDS 50.0%
ACCACATACTGTGCCGTGTA+AGG + chr1.3:17387022-17387041 MS.gene25178:CDS 50.0%
ACCTTACACGGCACAGTATG+TGG - chr1.3:17387026-17387045 None:intergenic 50.0%
ACTGTGCCGTGTAAGGTTAC+TGG + chr1.3:17387029-17387048 MS.gene25178:CDS 50.0%
AGGGGATCTACCAAAACCCT+TGG + chr1.3:17387173-17387192 MS.gene25178:CDS 50.0%
CCTTGATCTTGGAGGAGGAA+AGG + chr1.3:17388789-17388808 MS.gene25178:CDS 50.0%
CCTTTCCTCCTCCAAGATCA+AGG - chr1.3:17388792-17388811 None:intergenic 50.0%
CGAGTCTCCTTCCAGAATTC+AGG - chr1.3:17388710-17388729 None:intergenic 50.0%
GCAACAATACCAGAACCACG+AGG - chr1.3:17387092-17387111 None:intergenic 50.0%
GTTCAGAAACAAACCCGAGC+TGG + chr1.3:17386828-17386847 MS.gene25178:CDS 50.0%
GTTCGCGCTTCTTAGCATAC+AGG - chr1.3:17387658-17387677 None:intergenic 50.0%
GTTCTTTGATGGGGAGAGAG+TGG - chr1.3:17386746-17386765 None:intergenic 50.0%
GTTGGCAAAGCCCACAACAA+AGG + chr1.3:17388766-17388785 MS.gene25178:CDS 50.0%
TGCTGCTAGGGTTCCTAAGA+AGG + chr1.3:17387109-17387128 MS.gene25178:CDS 50.0%
! ATGCTAAGAAGCGCGAACAC+AGG + chr1.3:17387660-17387679 MS.gene25178:intron 50.0%
!! CCTGTGAGAAGAGGGTATTG+GGG + chr1.3:17386984-17387003 MS.gene25178:CDS 50.0%
!! GCTCGGGTTTGTTTCTGAAC+CGG - chr1.3:17386828-17386847 None:intergenic 50.0%
!!! AGACGGCCGAGTTTTGTTAC+TGG - chr1.3:17386681-17386700 None:intergenic 50.0%
!!! GACGGCCGAGTTTTGTTACT+GGG - chr1.3:17386680-17386699 None:intergenic 50.0%
ATACCAGAACCACGAGGAGC+AGG - chr1.3:17387086-17387105 None:intergenic 55.0%
CCCCAATACCCTCTTCTCAC+AGG - chr1.3:17386987-17387006 None:intergenic 55.0%
CTTTGATGGGGAGAGAGTGG+AGG - chr1.3:17386743-17386762 None:intergenic 55.0%
GATCTTGGAGGAGGAAAGGG+CGG + chr1.3:17388793-17388812 MS.gene25178:CDS 55.0%
TCCGAAACCACGTCCGAATC+CGG - chr1.3:17386568-17386587 None:intergenic 55.0%
ACCGGAGACAGAGCCGGATT+CGG + chr1.3:17386552-17386571 MS.gene25178:CDS 60.0%
ACCGGGTTCGTTGACCAGCT+CGG - chr1.3:17386845-17386864 None:intergenic 60.0%
ATGGCAGACCGAGGAGCTAC+CGG + chr1.3:17386534-17386553 MS.gene25178:CDS 60.0%
CGTATGGTTCCTGCTCCTCG+TGG + chr1.3:17387074-17387093 MS.gene25178:CDS 60.0%
CTCGGCCGTCTCGTGAAAGA+AGG + chr1.3:17386690-17386709 MS.gene25178:CDS 60.0%
CTCTGTCTCCGGTAGCTCCT+CGG - chr1.3:17386545-17386564 None:intergenic 60.0%
GCCGGATTCGGACGTGGTTT+CGG + chr1.3:17386564-17386583 MS.gene25178:CDS 60.0%
GGACGTGGTTTCGGAGATCG+TGG + chr1.3:17386573-17386592 MS.gene25178:CDS 60.0%
GGTTTCGGAGATCGTGGTCG+AGG + chr1.3:17386579-17386598 MS.gene25178:CDS 60.0%
GTTCCTGCTCCTCGTGGTTC+TGG + chr1.3:17387080-17387099 MS.gene25178:CDS 60.0%
TCCGAATCCGGCTCTGTCTC+CGG - chr1.3:17386556-17386575 None:intergenic 60.0%
TTCGGAGATCGTGGTCGAGG+TGG + chr1.3:17386582-17386601 MS.gene25178:CDS 60.0%
CCCGAGCTGGTCAACGAACC+CGG + chr1.3:17386841-17386860 MS.gene25178:CDS 65.0%
CCGGGTTCGTTGACCAGCTC+GGG - chr1.3:17386844-17386863 None:intergenic 65.0%
GACAGAGCCGGATTCGGACG+TGG + chr1.3:17386558-17386577 MS.gene25178:CDS 65.0%
GATCGTGGTCGAGGTGGCAG+AGG + chr1.3:17386588-17386607 MS.gene25178:CDS 65.0%
GGAGCTACCGGAGACAGAGC+CGG + chr1.3:17386546-17386565 MS.gene25178:CDS 65.0%
AGAGGCGGAGATCGTGGCCG+TGG + chr1.3:17386606-17386625 MS.gene25178:CDS 70.0%
CGTGGTCGAGGTGGCAGAGG+CGG + chr1.3:17386591-17386610 MS.gene25178:CDS 70.0%
GGTGGCAGAGGCGGAGATCG+TGG + chr1.3:17386600-17386619 MS.gene25178:CDS 70.0%
!! AGGCGCTGGCCGTCGTGAAG+AGG + chr1.3:17386641-17386660 MS.gene25178:CDS 70.0%
CACGACGGCCAGCGCCTCTG+CGG - chr1.3:17386638-17386657 None:intergenic 75.0%
CGCCTCTGCGGCGACCTCCA+CGG - chr1.3:17386626-17386645 None:intergenic 75.0%
GGCGGAGATCGTGGCCGTGG+AGG + chr1.3:17386609-17386628 MS.gene25178:CDS 75.0%
! GGCCGTGGAGGTCGCCGCAG+AGG + chr1.3:17386621-17386640 MS.gene25178:CDS 80.0%
!! GGAGGTCGCCGCAGAGGCGC+TGG + chr1.3:17386627-17386646 MS.gene25178:CDS 80.0%


Chromosome Type Strat End Strand Name
chr1.3 gene 17386534 17388823 17386534 ID=MS.gene25178
chr1.3 mRNA 17386534 17388823 17386534 ID=MS.gene25178.t1;Parent=MS.gene25178
chr1.3 exon 17386534 17387208 17386534 ID=MS.gene25178.t1.exon1;Parent=MS.gene25178.t1
chr1.3 CDS 17386534 17387208 17386534 ID=cds.MS.gene25178.t1;Parent=MS.gene25178.t1
chr1.3 exon 17388665 17388823 17388665 ID=MS.gene25178.t1.exon2;Parent=MS.gene25178.t1
chr1.3 CDS 17388665 17388823 17388665 ID=cds.MS.gene25178.t1;Parent=MS.gene25178.t1
Gene Sequence

>MS.gene25178

ATGGCAGACCGAGGAGCTACCGGAGACAGAGCCGGATTCGGACGTGGTTTCGGAGATCGTGGTCGAGGTGGCAGAGGCGGAGATCGTGGCCGTGGAGGTCGCCGCAGAGGCGCTGGCCGTCGTGAAGAGGAAGAAAAATGGGTCCCAGTAACAAAACTCGGCCGTCTCGTGAAAGAAGGCAAAATTCGCAGCTTAGAACAAATCTACCTCCACTCTCTCCCCATCAAAGAACACCAAATCATCGACACCTTAGTCGGTCCCACTTTGAAAGACGAAGTGATGAAAATCATGCCGGTTCAGAAACAAACCCGAGCTGGTCAACGAACCCGGTTCAAAGCTTTTGTAGTTGTGGGTGACAACAATGGTCATGTAGGGTTAGGTGTGAAGTGTAGTAAGGAAGTTGCTACTGCAATTCGCGGTGCGATTATTCTTGCGAAATTGAGTGTGATTCCTGTGAGAAGAGGGTATTGGGGAAATAAGATTGGGAAACCACATACTGTGCCGTGTAAGGTTACTGGAAAGTGTGGTTCTGTTACTGTTCGTATGGTTCCTGCTCCTCGTGGTTCTGGTATTGTTGCTGCTAGGGTTCCTAAGAAGGTTTTGCAGTTTGCTGGAATTGATGATGTTTTTACTTCTTCAAGGGGATCTACCAAAACCCTTGGCAATTTCGTTAAGGTCTGTTTTTTTTTTCTTCATATTTTAATTTGTTTTTCGTCTTTTATTTACTGTTTATGATTTTATGTTTACGGTTTATGATTTTATATATGAATTGTGATAATGATTTTTGATTTATATTACTTATATGAAATTATTGTGTCATTCATTTATTTGATTTTTAACTCATATATATTCATATGTAAAATGTCCTGTTTTATATATTCATTTATTTGAGTTGTATAATGTGAACCTGTTTGGATAAACAACTTATCTGCAGCTTATAGCACAACATTAAATTGTTTTGTAAATGCTATCTGTTTTCATAAGCCATATTGGAGAACTTATGGAAATTAAGTTGAAAAAATCTTATGAAAACTGTCTTAAGCTGATTTCATAAGTTCTCTCAAACAGTCTTACAAAATTTATGCTAGTAGATAAACTCGAATAAACCAATCACAATAGACCCTGTATGCTAAGAAGCGCGAACACAGGCACTAGATGCAATTAATCATTTGCAAAACTTGGTGTGATTATATGTAGCTAGGTTAGTGACAGTGTTAGTTTGGATAGATGCAATTTATCTACTATCAATTTACGAGATAGATGGACATAAGTGATAGGTTAGTGGGATCCTTTAAAATAGCGAAGTCACTAATGGGTCCTCTAATCTTCCACTTGTTTCTATCTATCTATTTCCCTTGAATGGACAGTAAATAGAAGCTAGATAAATGGAGCAAATCTTAGTAGACCTAGACATGCTTTCAAATGAAGTGTTGGTGCTAAATAGGTTTTATGTGTATACAAACTTGAGTGGTCAATCTTATTGTGAGTAAAATTTAGGATGTGACGTGTGAGTATAGTAAATTGATTTGAAAATAAATGATGGCTTTATTTCAATATTTTTTTTGGAGGAGGCTGGATATTTCAATATGATTGGTTATGTATGTATATTTGTATAATATAAATCTTTAATTTGTTCATCAATGGTTATATTACCCACTTTACATTTTAAATGGATTTGCTTATTTAGTAGAAGTCAGATCATAGAATTTAACTTGAAATGGAAATGTGTTGTTTATTTTTGAATGAAAATGTTCAATTATAATCAACAGACTTGTTGGGTTTTCAAAATCTAATAATTTTATGCATGTTTTATAAGATTTTAATTGTATATTATAAACAGGGCCCTTGGAATGATATTTTGAAATTGACTCCCTTGTGGTTAAGAACATTAAAATCTCATTTGTTTGTATAATCAAATGGTATTCTGTTTGTATTTTCCGCATATAAACTTTATTAGTTGTCATATATTGAGACAGTGCTGGATGAGTAGTTTGTGTTGGTTTGTTTCATTGGTTTATAGTTCTGGTCTTTTTTTTCCTGCAATCGAATCTATGTATGCCAGAATTTGTTACTCGTGTTTGTTCATTACTTTTGCTATAAACAGATTTATTAATGCTATTTATTTTTGCAGGCTACATTTGACTGTCTGATGAAGACTTATGGTTTCTTGACACCTGAATTCTGGAAGGAGACTCGCTTCAGTAAATCTCCATTCCAAGAGTACACTGATATGTTGGCAAAGCCCACAACAAAGGCCTTGATCTTGGAGGAGGAAAGGGCGGAAGCTTAA
Protein sequence

>MS.gene25178.t1

MADRGATGDRAGFGRGFGDRGRGGRGGDRGRGGRRRGAGRREEEEKWVPVTKLGRLVKEGKIRSLEQIYLHSLPIKEHQIIDTLVGPTLKDEVMKIMPVQKQTRAGQRTRFKAFVVVGDNNGHVGLGVKCSKEVATAIRGAIILAKLSVIPVRRGYWGNKIGKPHTVPCKVTGKCGSVTVRMVPAPRGSGIVAARVPKKVLQFAGIDDVFTSSRGSTKTLGNFVKATFDCLMKTYGFLTPEFWKETRFSKSPFQEYTDMLAKPTTKALILEEERAEA