AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene83062

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene83062.t1 MTR_1g032450 99.639 277 1 0 1 277 1 277 0.0 558
MS.gene83062.t1 MTR_8g105340 94.662 281 9 3 1 277 1 279 0.0 524
MS.gene83062.t1 MTR_8g105340 98.729 236 3 0 42 277 35 270 5.24e-173 478
MS.gene83062.t1 MTR_4g088615 32.727 165 94 4 89 244 147 303 2.36e-15 75.1
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene83062.t1 AT3G57490 92.308 221 17 0 43 263 41 261 2.31e-152 426
MS.gene83062.t1 AT2G41840 90.308 227 19 1 42 268 49 272 4.66e-151 424
MS.gene83062.t1 AT1G58684 87.712 236 26 1 43 275 49 284 2.68e-150 422
MS.gene83062.t1 AT1G58983 87.712 236 26 1 43 275 49 284 2.68e-150 422
MS.gene83062.t1 AT1G59359 87.712 236 26 1 43 275 49 284 2.68e-150 422
MS.gene83062.t1 AT1G58380 87.342 237 27 1 42 275 48 284 3.38e-150 421
MS.gene83062.t1 AT2G33800 29.530 149 97 2 89 237 147 287 2.33e-14 72.0

Find 97 sgRNAs with CRISPR-Local

Find 167 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GTTTCTTGACACCTGAATTC+TGG 0.269557 1.2:+16967872 MS.gene83062:CDS
GCAAACTGCAAAACCTTCTT+AGG 0.278482 1.2:-16966349 None:intergenic
AACGAAATTGCCAAGGGTTT+TGG 0.290170 1.2:-16966410 None:intergenic
GCCGGATTCGGAAGAGGTTT+CGG 0.325361 1.2:+16965791 MS.gene83062:CDS
GACTGTCTGATGAAGACTTA+TGG 0.329475 1.2:+16967850 MS.gene83062:CDS
CCGGGTTCGTTGACCGGCTC+TGG 0.332475 1.2:-16966068 None:intergenic
CGAGTCTCCTTCCAGAATTC+AGG 0.345000 1.2:-16967883 None:intergenic
TCCGAAACCTCTTCCGAATC+CGG 0.356237 1.2:-16965792 None:intergenic
GATGATTTGGTGTTCTTTGA+TGG 0.360586 1.2:-16965981 None:intergenic
GTTCCTGCACCGAGAGGTTC+TGG 0.361388 1.2:+16966307 MS.gene83062:CDS
GGAGGCCGCCGCAGAGGCGC+TGG 0.365196 1.2:+16965854 MS.gene83062:CDS
CGCCTCTGCGGCGGCCTCCA+CGG 0.366930 1.2:-16965850 None:intergenic
CTACTAAGGTATCGATGATT+TGG 0.379765 1.2:-16965994 None:intergenic
CCAACATATCAGTGTACTCT+TGG 0.379970 1.2:-16967924 None:intergenic
CACAACAAAGGCCTTGATCT+TGG 0.385331 1.2:+16967954 MS.gene83062:CDS
TTCTGGTATTGTTGCTGCTA+GGG 0.392766 1.2:+16966324 MS.gene83062:CDS
TTCAAAGCCTTTGTCGTTGT+GGG 0.395273 1.2:+16966091 MS.gene83062:CDS
ACTGTGCCGTGTAAGGTTAC+TGG 0.397057 1.2:+16966256 MS.gene83062:CDS
AAGATCAAGGCCTTTGTTGT+GGG 0.399949 1.2:-16967952 None:intergenic
AGACGGCCGAGCTTTGTTAC+TGG 0.409258 1.2:-16965905 None:intergenic
AACCCTTGGCAATTTCGTTA+AGG 0.410713 1.2:+16966414 MS.gene83062:CDS
CAAGATCAAGGCCTTTGTTG+TGG 0.418524 1.2:-16967953 None:intergenic
TGCTGCTAGGGTTCCTAAGA+AGG 0.419749 1.2:+16966336 MS.gene83062:CDS
TTTGAACCGGGTTCGTTGAC+CGG 0.422499 1.2:-16966074 None:intergenic
TTCCTGTGAGAAGAGGTTAC+TGG 0.425027 1.2:+16966209 MS.gene83062:CDS
ATCACTTCGTCTTTCAGAGT+GGG 0.431632 1.2:-16966019 None:intergenic
CAACGACAAAGGCTTTGAAC+CGG 0.439377 1.2:-16966087 None:intergenic
ACCGGAGACAGAGCCGGATT+CGG 0.440482 1.2:+16965779 MS.gene83062:CDS
TCCGAATCCGGCTCTGTCTC+CGG 0.446226 1.2:-16965780 None:intergenic
CTCTGTCTCCGGTAGCTCCT+CGG 0.448842 1.2:-16965769 None:intergenic
GCTCTGGTTTGTTTCTGAAC+CGG 0.455324 1.2:-16966052 None:intergenic
GACGGCCGAGCTTTGTTACT+GGG 0.470512 1.2:-16965904 None:intergenic
GTTCTGGTATTGTTGCTGCT+AGG 0.471297 1.2:+16966323 MS.gene83062:CDS
CTTGATCTTGGAGGAGGAAA+GGG 0.473493 1.2:+16967966 MS.gene83062:CDS
TCCTGTGAGAAGAGGTTACT+GGG 0.474658 1.2:+16966210 MS.gene83062:CDS
GTTCAAAGCCTTTGTCGTTG+TGG 0.481091 1.2:+16966090 MS.gene83062:CDS
GCCACATACTGTGCCGTGTA+AGG 0.481615 1.2:+16966249 MS.gene83062:CDS
TTTCAGAGTGGGGCCTACTA+AGG 0.486712 1.2:-16966008 None:intergenic
GGCCGTGGAGGCCGCCGCAG+AGG 0.493445 1.2:+16965848 MS.gene83062:CDS
CAAATCATCGATACCTTAGT+AGG 0.501293 1.2:+16965995 MS.gene83062:CDS
GACAGAGCCGGATTCGGAAG+AGG 0.501973 1.2:+16965785 MS.gene83062:CDS
CTCGGCCGTCTCGTCAAAGA+AGG 0.518865 1.2:+16965917 MS.gene83062:CDS
CCTTTCCTCCTCCAAGATCA+AGG 0.523177 1.2:-16967965 None:intergenic
AGAGGCGGAGATCGTGGCCG+TGG 0.527803 1.2:+16965833 MS.gene83062:CDS
ACCTTACACGGCACAGTATG+TGG 0.528460 1.2:-16966250 None:intergenic
TGGTTCTGTTACTGTTCGTA+TGG 0.533362 1.2:+16966285 MS.gene83062:CDS
CCAAGAGTACACTGATATGT+TGG 0.536690 1.2:+16967924 MS.gene83062:CDS
CGTGGTCGAGGTGGCAGAGG+CGG 0.538891 1.2:+16965818 MS.gene83062:CDS
CACTTTCCAGTAACCTTACA+CGG 0.538940 1.2:-16966262 None:intergenic
ATACCAGAACCTCTCGGTGC+AGG 0.541543 1.2:-16966310 None:intergenic
AACGACAAAGGCTTTGAACC+GGG 0.543012 1.2:-16966086 None:intergenic
ATGGCAGACCGAGGAGCTAC+CGG 0.543216 1.2:+16965761 MS.gene83062:CDS
CATCACTTCGTCTTTCAGAG+TGG 0.543843 1.2:-16966020 None:intergenic
AGGCGCTGGCCGTCGTGAAG+AGG 0.545371 1.2:+16965868 MS.gene83062:CDS
GGTTTCGGAGATCGTGGTCG+AGG 0.546990 1.2:+16965806 MS.gene83062:CDS
CTTGACACCTGAATTCTGGA+AGG 0.547375 1.2:+16967876 MS.gene83062:CDS
CCTTGATCTTGGAGGAGGAA+AGG 0.547470 1.2:+16967965 MS.gene83062:CDS
AACAAAGGCCTTGATCTTGG+AGG 0.548080 1.2:+16967957 MS.gene83062:CDS
GATCGTGGTCGAGGTGGCAG+AGG 0.555874 1.2:+16965815 MS.gene83062:CDS
ATATCAGTGTACTCTTGGAA+TGG 0.556129 1.2:-16967919 None:intergenic
CCAGAGCCGGTCAACGAACC+CGG 0.560802 1.2:+16966068 MS.gene83062:CDS
ATGATTTGGTGTTCTTTGAT+GGG 0.563159 1.2:-16965980 None:intergenic
AGGGGATCTACCAAAACCCT+TGG 0.565634 1.2:+16966400 MS.gene83062:CDS
GGAGCTACCGGAGACAGAGC+CGG 0.566711 1.2:+16965773 MS.gene83062:CDS
AAAGGCCTTGATCTTGGAGG+AGG 0.570185 1.2:+16967960 MS.gene83062:CDS
TCACTTCGTCTTTCAGAGTG+GGG 0.570297 1.2:-16966018 None:intergenic
CCCCAGTAACCTCTTCTCAC+AGG 0.570541 1.2:-16966211 None:intergenic
GAAGTTGCTACTGCAATTCG+CGG 0.571598 1.2:+16966157 MS.gene83062:CDS
TGGGTCCCAGTAACAAAGCT+CGG 0.574303 1.2:+16965899 MS.gene83062:CDS
GGTGACAACAATGGTCATGT+TGG 0.579247 1.2:+16966112 MS.gene83062:CDS
GCTAGGTGTGAAGTGCAGTA+AGG 0.579415 1.2:+16966135 MS.gene83062:CDS
GTGACAACAATGGTCATGTT+GGG 0.579633 1.2:+16966113 MS.gene83062:CDS
GGTGGCAGAGGCGGAGATCG+TGG 0.589603 1.2:+16965827 MS.gene83062:CDS
GTTGTCACCCACAACGACAA+AGG 0.596166 1.2:-16966098 None:intergenic
GTTCTTTGATGGGAAGAGAG+TGG 0.601081 1.2:-16965970 None:intergenic
AGACCTTAACGAAATTGCCA+AGG 0.604222 1.2:-16966417 None:intergenic
GGTAGATTTGTTCTAAGCTG+CGG 0.604842 1.2:-16965946 None:intergenic
CTTTGATGGGAAGAGAGTGG+AGG 0.605151 1.2:-16965967 None:intergenic
CGAAGTGATGAAAATCATGC+CGG 0.605193 1.2:+16966033 MS.gene83062:CDS
GTTCAGAAACAAACCAGAGC+CGG 0.607188 1.2:+16966055 MS.gene83062:CDS
GTTGGCAAAGCCCACAACAA+AGG 0.608366 1.2:+16967942 MS.gene83062:CDS
GACCTTAACGAAATTGCCAA+GGG 0.610712 1.2:-16966416 None:intergenic
CACGACGGCCAGCGCCTCTG+CGG 0.615990 1.2:-16965862 None:intergenic
GGAAGAGGTTTCGGAGATCG+TGG 0.617372 1.2:+16965800 MS.gene83062:CDS
AACAATGGTCATGTTGGGCT+AGG 0.617527 1.2:+16966118 MS.gene83062:CDS
GACGGCCAGCGCCTCTGCGG+CGG 0.619513 1.2:-16965859 None:intergenic
TTCGGAGATCGTGGTCGAGG+TGG 0.626828 1.2:+16965809 MS.gene83062:CDS
GTCGTTGTGGGTGACAACAA+TGG 0.631670 1.2:+16966103 MS.gene83062:CDS
AGTGTGATTCCTGTGAGAAG+AGG 0.634613 1.2:+16966202 MS.gene83062:CDS
TGTAAGGTTACTGGAAAGTG+TGG 0.643088 1.2:+16966265 MS.gene83062:CDS
GCAACAATACCAGAACCTCT+CGG 0.646729 1.2:-16966316 None:intergenic
GGCGGAGATCGTGGCCGTGG+AGG 0.662594 1.2:+16965836 MS.gene83062:CDS
CGTATGGTTCCTGCACCGAG+AGG 0.664454 1.2:+16966301 MS.gene83062:CDS
GATCTTGGAGGAGGAAAGGG+CGG 0.671501 1.2:+16967969 MS.gene83062:CDS
CTGTGAGAAGAGGTTACTGG+GGG 0.685323 1.2:+16966212 MS.gene83062:CDS
CAACCAACAATGGCAGACCG+AGG 0.687866 1.2:+16965752 None:intergenic
CCTGTGAGAAGAGGTTACTG+GGG 0.696718 1.2:+16966211 MS.gene83062:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAAAACAAATTAAAATATGA+AGG - chr1.2:16966453-16966472 None:intergenic 10.0%
!! AATAAATGAATATATAAAAC+AGG - chr1.2:16966583-16966602 None:intergenic 10.0%
!! AATTAATCATTTGCAAAATT+TGG + chr1.2:16966879-16966898 MS.gene83062:intron 15.0%
!! TCAATTGTATATTATAAACA+GGG + chr1.2:16967528-16967547 MS.gene83062:intron 15.0%
!! TTCAATTGTATATTATAAAC+AGG + chr1.2:16967527-16967546 MS.gene83062:intron 15.0%
!!! AATTTTAAAATATCATTCCA+AGG - chr1.2:16967556-16967575 None:intergenic 15.0%
!!! ATTTTAAAATATCATTCCAA+GGG - chr1.2:16967555-16967574 None:intergenic 15.0%
!!! CTTTATTTCAATATTTTTGT+TGG + chr1.2:16967263-16967282 MS.gene83062:intron 15.0%
!! CAACTAATAAAGTTTATATG+CGG - chr1.2:16967650-16967669 None:intergenic 20.0%
!! CAATTGTATATTATAAACAG+GGG + chr1.2:16967529-16967548 MS.gene83062:intron 20.0%
!! CATTTGTTTGTATAATCAAA+TGG + chr1.2:16967607-16967626 MS.gene83062:intron 20.0%
!! TCTTATTGTGAGTAAAATTT+AGG + chr1.2:16967195-16967214 MS.gene83062:intron 20.0%
!!! AATCCATTTAAAATGTAAAG+TGG - chr1.2:16967376-16967395 None:intergenic 20.0%
!!! ATCCATTTAAAATGTAAAGT+GGG - chr1.2:16967375-16967394 None:intergenic 20.0%
!!! ATCTTTAATTTGTTCATCAA+TGG + chr1.2:16967341-16967360 MS.gene83062:intron 20.0%
!!! ATTGATTTGAAAATAAACGA+TGG + chr1.2:16967240-16967259 MS.gene83062:intron 20.0%
!!! TATTTCAATATTTTTGTTGG+AGG + chr1.2:16967266-16967285 MS.gene83062:intron 20.0%
!!! TCATAGAATTTAACTTGAAA+TGG + chr1.2:16967418-16967437 MS.gene83062:intron 20.0%
!!! TTAATGCTATTTATTTTTGC+AGG + chr1.2:16967819-16967838 MS.gene83062:intron 20.0%
!!! TTTATTTGAGTTGTATAATG+TGG + chr1.2:16966597-16966616 MS.gene83062:intron 20.0%
! AATAGATAGATAGAAACAAG+TGG - chr1.2:16967051-16967070 None:intergenic 25.0%
! GTAAATAGAAGCTAGATAAA+TGG + chr1.2:16967086-16967105 MS.gene83062:intron 25.0%
! TTTAATGTTCTTAACCACAA+GGG - chr1.2:16967583-16967602 None:intergenic 25.0%
!! TACCCACTTTACATTTTAAA+TGG + chr1.2:16967370-16967389 MS.gene83062:intron 25.0%
!! TTTTAATGTTCTTAACCACA+AGG - chr1.2:16967584-16967603 None:intergenic 25.0%
!!! ATGATGTTTTTACTTCTTCA+AGG + chr1.2:16966380-16966399 MS.gene83062:CDS 25.0%
!!! TGATGTTTTTACTTCTTCAA+GGG + chr1.2:16966381-16966400 MS.gene83062:CDS 25.0%
AAAATAGCGAAGTCACTAAT+GGG + chr1.2:16967013-16967032 MS.gene83062:intron 30.0%
AAGCTATATTGGAGAACTTA+TGG + chr1.2:16966700-16966719 MS.gene83062:intron 30.0%
ATCTATCTATTTCCCTTGAA+TGG + chr1.2:16967060-16967079 MS.gene83062:intron 30.0%
CTATCAATTTACGAGATAGA+TGG + chr1.2:16966961-16966980 MS.gene83062:intron 30.0%
GATAAGTTGTTTATCCAAAC+AGG - chr1.2:16966624-16966643 None:intergenic 30.0%
GCTGGATATTTCAATATGAT+TGG + chr1.2:16967291-16967310 MS.gene83062:intron 30.0%
TAAAATAGCGAAGTCACTAA+TGG + chr1.2:16967012-16967031 MS.gene83062:intron 30.0%
TCTATTTACTGTCCATTCAA+GGG - chr1.2:16967075-16967094 None:intergenic 30.0%
TTCTATTTACTGTCCATTCA+AGG - chr1.2:16967076-16967095 None:intergenic 30.0%
!! ATGATTTGGTGTTCTTTGAT+GGG - chr1.2:16965983-16966002 None:intergenic 30.0%
!! GATGTTTTTACTTCTTCAAG+GGG + chr1.2:16966382-16966401 MS.gene83062:CDS 30.0%
!! GCTGTTTTCATAAGCTATAT+TGG + chr1.2:16966689-16966708 MS.gene83062:intron 30.0%
!! GTTTCATTGGTTTATAGTTC+TGG + chr1.2:16967713-16967732 MS.gene83062:intron 30.0%
!! TAGTGACTTCGCTATTTTAA+AGG - chr1.2:16967011-16967030 None:intergenic 30.0%
!! TTCAATATTTTTGTTGGAGG+AGG + chr1.2:16967269-16967288 MS.gene83062:intron 30.0%
!! TTTGTGTTGGTTTGTTTCAT+TGG + chr1.2:16967700-16967719 MS.gene83062:intron 30.0%
!!! TTTTAAAATTGACTCCCTTG+TGG + chr1.2:16967566-16967585 MS.gene83062:intron 30.0%
AGAAACAAGTGGAAGATTAG+AGG - chr1.2:16967040-16967059 None:intergenic 35.0%
ATATCAGTGTACTCTTGGAA+TGG - chr1.2:16967922-16967941 None:intergenic 35.0%
ATTACAGGGTCTATTGTGAT+TGG - chr1.2:16966828-16966847 None:intergenic 35.0%
CAAACACGAGTAACAAATTC+TGG - chr1.2:16967771-16967790 None:intergenic 35.0%
CAAATCATCGATACCTTAGT+AGG + chr1.2:16965995-16966014 MS.gene83062:CDS 35.0%
CATGCTTTCAAATGAAGTGT+TGG + chr1.2:16967131-16967150 MS.gene83062:intron 35.0%
GACATAAGTGATAGGTTAGT+GGG + chr1.2:16966983-16967002 MS.gene83062:intron 35.0%
GATAGATGGACATAAGTGAT+AGG + chr1.2:16966975-16966994 MS.gene83062:intron 35.0%
TATGTGTGTACAAACTTGAG+TGG + chr1.2:16967168-16967187 MS.gene83062:intron 35.0%
TCGTGAAGAGGAAGAAAAAT+GGG + chr1.2:16965880-16965899 MS.gene83062:CDS 35.0%
TGGTGTGATTATATGTAGCT+AGG + chr1.2:16966899-16966918 MS.gene83062:intron 35.0%
TTGTATAATGTGGACCTGTT+TGG + chr1.2:16966607-16966626 MS.gene83062:intron 35.0%
! ATTTTTCTTCCTCTTCACGA+CGG - chr1.2:16965880-16965899 None:intergenic 35.0%
! CTTCATTTGAAAGCATGTCT+AGG - chr1.2:16967128-16967147 None:intergenic 35.0%
!! CTACTAAGGTATCGATGATT+TGG - chr1.2:16965997-16966016 None:intergenic 35.0%
!! GATGATTTGGTGTTCTTTGA+TGG - chr1.2:16965984-16966003 None:intergenic 35.0%
AACCCTTGGCAATTTCGTTA+AGG + chr1.2:16966414-16966433 MS.gene83062:CDS 40.0%
AGACCTTAACGAAATTGCCA+AGG - chr1.2:16966420-16966439 None:intergenic 40.0%
ATATTATAAACAGGGGCCCT+TGG + chr1.2:16967536-16967555 MS.gene83062:intron 40.0%
ATCACTTCGTCTTTCAGAGT+GGG - chr1.2:16966022-16966041 None:intergenic 40.0%
CACTTTCCAGTAACCTTACA+CGG - chr1.2:16966265-16966284 None:intergenic 40.0%
CCAACATATCAGTGTACTCT+TGG - chr1.2:16967927-16967946 None:intergenic 40.0%
CGAAGTGATGAAAATCATGC+CGG + chr1.2:16966033-16966052 MS.gene83062:CDS 40.0%
GACCTTAACGAAATTGCCAA+GGG - chr1.2:16966419-16966438 None:intergenic 40.0%
GACTGTCTGATGAAGACTTA+TGG + chr1.2:16967850-16967869 MS.gene83062:CDS 40.0%
GCAAACTGCAAAACCTTCTT+AGG - chr1.2:16966352-16966371 None:intergenic 40.0%
GCATACATAGATTCGATTGC+AGG - chr1.2:16967749-16967768 None:intergenic 40.0%
GGACATAAGTGATAGGTTAG+TGG + chr1.2:16966982-16967001 MS.gene83062:intron 40.0%
GGTTAGTGACAGTGTTAGTT+TGG + chr1.2:16966920-16966939 MS.gene83062:intron 40.0%
GTCATATATTGAGACAGTGC+TGG + chr1.2:16967669-16967688 MS.gene83062:intron 40.0%
GTCGTGAAGAGGAAGAAAAA+TGG + chr1.2:16965879-16965898 MS.gene83062:CDS 40.0%
GTGACAACAATGGTCATGTT+GGG + chr1.2:16966113-16966132 MS.gene83062:CDS 40.0%
GTTACTGGGGGAATAAGATT+GGG + chr1.2:16966224-16966243 MS.gene83062:CDS 40.0%
GTTTCTTGACACCTGAATTC+TGG + chr1.2:16967872-16967891 MS.gene83062:CDS 40.0%
TGTAAGGTTACTGGAAAGTG+TGG + chr1.2:16966265-16966284 MS.gene83062:CDS 40.0%
TTCAAAGCCTTTGTCGTTGT+GGG + chr1.2:16966091-16966110 MS.gene83062:CDS 40.0%
TTCTGGTATTGTTGCTGCTA+GGG + chr1.2:16966324-16966343 MS.gene83062:CDS 40.0%
! AACGAAATTGCCAAGGGTTT+TGG - chr1.2:16966413-16966432 None:intergenic 40.0%
! AAGAAGGTTTTGCAGTTTGC+TGG + chr1.2:16966352-16966371 MS.gene83062:CDS 40.0%
! AAGATCAAGGCCTTTGTTGT+GGG - chr1.2:16967955-16967974 None:intergenic 40.0%
! CCAAGAGTACACTGATATGT+TGG + chr1.2:16967924-16967943 MS.gene83062:CDS 40.0%
! GGTAGATTTGTTCTAAGCTG+CGG - chr1.2:16965949-16965968 None:intergenic 40.0%
! TGGTTCTGTTACTGTTCGTA+TGG + chr1.2:16966285-16966304 MS.gene83062:CDS 40.0%
! TTTTGCCTTCTTTGACGAGA+CGG - chr1.2:16965925-16965944 None:intergenic 40.0%
!! ATATTTTTGTTGGAGGAGGC+TGG + chr1.2:16967273-16967292 MS.gene83062:intron 40.0%
!! ATGAAGTGTTGGCGCTAAAT+AGG + chr1.2:16967142-16967161 MS.gene83062:intron 40.0%
AACAAAGGCCTTGATCTTGG+AGG + chr1.2:16967957-16967976 MS.gene83062:CDS 45.0%
AACAATGGTCATGTTGGGCT+AGG + chr1.2:16966118-16966137 MS.gene83062:CDS 45.0%
AGTGTGATTCCTGTGAGAAG+AGG + chr1.2:16966202-16966221 MS.gene83062:CDS 45.0%
CAAGATCAAGGCCTTTGTTG+TGG - chr1.2:16967956-16967975 None:intergenic 45.0%
CACAACAAAGGCCTTGATCT+TGG + chr1.2:16967954-16967973 MS.gene83062:CDS 45.0%
CATCACTTCGTCTTTCAGAG+TGG - chr1.2:16966023-16966042 None:intergenic 45.0%
CTTGACACCTGAATTCTGGA+AGG + chr1.2:16967876-16967895 MS.gene83062:CDS 45.0%
CTTGATCTTGGAGGAGGAAA+GGG + chr1.2:16967966-16967985 MS.gene83062:CDS 45.0%
GAAGTTGCTACTGCAATTCG+CGG + chr1.2:16966157-16966176 MS.gene83062:CDS 45.0%
GCAACAATACCAGAACCTCT+CGG - chr1.2:16966319-16966338 None:intergenic 45.0%
GGTGACAACAATGGTCATGT+TGG + chr1.2:16966112-16966131 MS.gene83062:CDS 45.0%
GGTTACTGGGGGAATAAGAT+TGG + chr1.2:16966223-16966242 MS.gene83062:CDS 45.0%
GTTCAAAGCCTTTGTCGTTG+TGG + chr1.2:16966090-16966109 MS.gene83062:CDS 45.0%
GTTCAGAAACAAACCAGAGC+CGG + chr1.2:16966055-16966074 MS.gene83062:CDS 45.0%
GTTCTGGTATTGTTGCTGCT+AGG + chr1.2:16966323-16966342 MS.gene83062:CDS 45.0%
GTTCTTTGATGGGAAGAGAG+TGG - chr1.2:16965973-16965992 None:intergenic 45.0%
TCACTTCGTCTTTCAGAGTG+GGG - chr1.2:16966021-16966040 None:intergenic 45.0%
TCCTGTGAGAAGAGGTTACT+GGG + chr1.2:16966210-16966229 MS.gene83062:CDS 45.0%
TCGCGCTTCTTAGCATTACA+GGG - chr1.2:16966842-16966861 None:intergenic 45.0%
TTCCTGTGAGAAGAGGTTAC+TGG + chr1.2:16966209-16966228 MS.gene83062:CDS 45.0%
TTCGCGCTTCTTAGCATTAC+AGG - chr1.2:16966843-16966862 None:intergenic 45.0%
! GCTGGATGAGTAGTTTGTGT+TGG + chr1.2:16967687-16967706 MS.gene83062:intron 45.0%
!! AACGACAAAGGCTTTGAACC+GGG - chr1.2:16966089-16966108 None:intergenic 45.0%
!! CAACGACAAAGGCTTTGAAC+CGG - chr1.2:16966090-16966109 None:intergenic 45.0%
!! GCTCTGGTTTGTTTCTGAAC+CGG - chr1.2:16966055-16966074 None:intergenic 45.0%
AAAGGCCTTGATCTTGGAGG+AGG + chr1.2:16967960-16967979 MS.gene83062:CDS 50.0%
ACCTTACACGGCACAGTATG+TGG - chr1.2:16966253-16966272 None:intergenic 50.0%
ACTGTGCCGTGTAAGGTTAC+TGG + chr1.2:16966256-16966275 MS.gene83062:CDS 50.0%
AGGGGATCTACCAAAACCCT+TGG + chr1.2:16966400-16966419 MS.gene83062:CDS 50.0%
CCTGTGAGAAGAGGTTACTG+GGG + chr1.2:16966211-16966230 MS.gene83062:CDS 50.0%
CCTTGATCTTGGAGGAGGAA+AGG + chr1.2:16967965-16967984 MS.gene83062:CDS 50.0%
CCTTTCCTCCTCCAAGATCA+AGG - chr1.2:16967968-16967987 None:intergenic 50.0%
CGAGTCTCCTTCCAGAATTC+AGG - chr1.2:16967886-16967905 None:intergenic 50.0%
CTGTGAGAAGAGGTTACTGG+GGG + chr1.2:16966212-16966231 MS.gene83062:CDS 50.0%
CTTTGATGGGAAGAGAGTGG+AGG - chr1.2:16965970-16965989 None:intergenic 50.0%
GCTAGGTGTGAAGTGCAGTA+AGG + chr1.2:16966135-16966154 MS.gene83062:CDS 50.0%
GTCGTTGTGGGTGACAACAA+TGG + chr1.2:16966103-16966122 MS.gene83062:CDS 50.0%
GTTGGCAAAGCCCACAACAA+AGG + chr1.2:16967942-16967961 MS.gene83062:CDS 50.0%
GTTGTCACCCACAACGACAA+AGG - chr1.2:16966101-16966120 None:intergenic 50.0%
TCCGAAACCTCTTCCGAATC+CGG - chr1.2:16965795-16965814 None:intergenic 50.0%
TGCTGCTAGGGTTCCTAAGA+AGG + chr1.2:16966336-16966355 MS.gene83062:CDS 50.0%
TGGGTCCCAGTAACAAAGCT+CGG + chr1.2:16965899-16965918 MS.gene83062:CDS 50.0%
TTTCAGAGTGGGGCCTACTA+AGG - chr1.2:16966011-16966030 None:intergenic 50.0%
TTTGAACCGGGTTCGTTGAC+CGG - chr1.2:16966077-16966096 None:intergenic 50.0%
! ATGCTAAGAAGCGCGAACAC+AGG + chr1.2:16966846-16966865 MS.gene83062:intron 50.0%
CCCCAGTAACCTCTTCTCAC+AGG - chr1.2:16966214-16966233 None:intergenic 55.0%
GATCTTGGAGGAGGAAAGGG+CGG + chr1.2:16967969-16967988 MS.gene83062:CDS 55.0%
GCCACATACTGTGCCGTGTA+AGG + chr1.2:16966249-16966268 MS.gene83062:CDS 55.0%
GCCGGATTCGGAAGAGGTTT+CGG + chr1.2:16965791-16965810 MS.gene83062:CDS 55.0%
GGAAGAGGTTTCGGAGATCG+TGG + chr1.2:16965800-16965819 MS.gene83062:CDS 55.0%
! ATACCAGAACCTCTCGGTGC+AGG - chr1.2:16966313-16966332 None:intergenic 55.0%
!! AGACGGCCGAGCTTTGTTAC+TGG - chr1.2:16965908-16965927 None:intergenic 55.0%
!! GACGGCCGAGCTTTGTTACT+GGG - chr1.2:16965907-16965926 None:intergenic 55.0%
ACCGGAGACAGAGCCGGATT+CGG + chr1.2:16965779-16965798 MS.gene83062:CDS 60.0%
ATGGCAGACCGAGGAGCTAC+CGG + chr1.2:16965761-16965780 MS.gene83062:CDS 60.0%
CGTATGGTTCCTGCACCGAG+AGG + chr1.2:16966301-16966320 MS.gene83062:CDS 60.0%
CTCGGCCGTCTCGTCAAAGA+AGG + chr1.2:16965917-16965936 MS.gene83062:CDS 60.0%
CTCTGTCTCCGGTAGCTCCT+CGG - chr1.2:16965772-16965791 None:intergenic 60.0%
GACAGAGCCGGATTCGGAAG+AGG + chr1.2:16965785-16965804 MS.gene83062:CDS 60.0%
GGTTTCGGAGATCGTGGTCG+AGG + chr1.2:16965806-16965825 MS.gene83062:CDS 60.0%
GTTCCTGCACCGAGAGGTTC+TGG + chr1.2:16966307-16966326 MS.gene83062:CDS 60.0%
TCCGAATCCGGCTCTGTCTC+CGG - chr1.2:16965783-16965802 None:intergenic 60.0%
TTCGGAGATCGTGGTCGAGG+TGG + chr1.2:16965809-16965828 MS.gene83062:CDS 60.0%
CCAGAGCCGGTCAACGAACC+CGG + chr1.2:16966068-16966087 MS.gene83062:CDS 65.0%
GATCGTGGTCGAGGTGGCAG+AGG + chr1.2:16965815-16965834 MS.gene83062:CDS 65.0%
GGAGCTACCGGAGACAGAGC+CGG + chr1.2:16965773-16965792 MS.gene83062:CDS 65.0%
AGAGGCGGAGATCGTGGCCG+TGG + chr1.2:16965833-16965852 MS.gene83062:CDS 70.0%
CGTGGTCGAGGTGGCAGAGG+CGG + chr1.2:16965818-16965837 MS.gene83062:CDS 70.0%
GGTGGCAGAGGCGGAGATCG+TGG + chr1.2:16965827-16965846 MS.gene83062:CDS 70.0%
! CCGGGTTCGTTGACCGGCTC+TGG - chr1.2:16966071-16966090 None:intergenic 70.0%
!! AGGCGCTGGCCGTCGTGAAG+AGG + chr1.2:16965868-16965887 MS.gene83062:CDS 70.0%
CACGACGGCCAGCGCCTCTG+CGG - chr1.2:16965865-16965884 None:intergenic 75.0%
GGCGGAGATCGTGGCCGTGG+AGG + chr1.2:16965836-16965855 MS.gene83062:CDS 75.0%
! CGCCTCTGCGGCGGCCTCCA+CGG - chr1.2:16965853-16965872 None:intergenic 80.0%
! GACGGCCAGCGCCTCTGCGG+CGG - chr1.2:16965862-16965881 None:intergenic 80.0%
! GGCCGTGGAGGCCGCCGCAG+AGG + chr1.2:16965848-16965867 MS.gene83062:CDS 85.0%
!! GGAGGCCGCCGCAGAGGCGC+TGG + chr1.2:16965854-16965873 MS.gene83062:CDS 85.0%


Chromosome Type Strat End Strand Name
chr1.2 gene 16965761 16967999 16965761 ID=MS.gene83062
chr1.2 mRNA 16965761 16967999 16965761 ID=MS.gene83062.t1;Parent=MS.gene83062
chr1.2 exon 16965761 16966435 16965761 ID=MS.gene83062.t1.exon1;Parent=MS.gene83062.t1
chr1.2 CDS 16965761 16966435 16965761 ID=cds.MS.gene83062.t1;Parent=MS.gene83062.t1
chr1.2 exon 16967841 16967999 16967841 ID=MS.gene83062.t1.exon2;Parent=MS.gene83062.t1
chr1.2 CDS 16967841 16967999 16967841 ID=cds.MS.gene83062.t1;Parent=MS.gene83062.t1
Gene Sequence

>MS.gene83062

ATGGCAGACCGAGGAGCTACCGGAGACAGAGCCGGATTCGGAAGAGGTTTCGGAGATCGTGGTCGAGGTGGCAGAGGCGGAGATCGTGGCCGTGGAGGCCGCCGCAGAGGCGCTGGCCGTCGTGAAGAGGAAGAAAAATGGGTCCCAGTAACAAAGCTCGGCCGTCTCGTCAAAGAAGGCAAAATCCGCAGCTTAGAACAAATCTACCTCCACTCTCTTCCCATCAAAGAACACCAAATCATCGATACCTTAGTAGGCCCCACTCTGAAAGACGAAGTGATGAAAATCATGCCGGTTCAGAAACAAACCAGAGCCGGTCAACGAACCCGGTTCAAAGCCTTTGTCGTTGTGGGTGACAACAATGGTCATGTTGGGCTAGGTGTGAAGTGCAGTAAGGAAGTTGCTACTGCAATTCGCGGTGCGATTATTCTTGCGAAATTGAGTGTGATTCCTGTGAGAAGAGGTTACTGGGGGAATAAGATTGGGAAGCCACATACTGTGCCGTGTAAGGTTACTGGAAAGTGTGGTTCTGTTACTGTTCGTATGGTTCCTGCACCGAGAGGTTCTGGTATTGTTGCTGCTAGGGTTCCTAAGAAGGTTTTGCAGTTTGCTGGAATTGATGATGTTTTTACTTCTTCAAGGGGATCTACCAAAACCCTTGGCAATTTCGTTAAGGTCTGTTTTTTTTTCCTTCATATTTTAATTTGTTTTTCTGTCTTTTATTTACTGTTTATGATTTTATATATGAATTGTGATAATGATTTTTGATTTATATTACATATTTGATTTTTAACTCATATATATTCATATGTAAAATGTCCTGTTTTATATATTCATTTATTTGAGTTGTATAATGTGGACCTGTTTGGATAAACAACTTATCTGCAGCTTATAGCACAACATTAAATTGTTTTGTAAATGCTTATAAGCTGTTTTCATAAGCTATATTGGAGAACTTATGGAAATTAAATTGAAAAAATCTTATGAAAACTGTCTTAAGCTGATTTCATAAGTTCTCTCGAACAGTCTTACAAAATTTATGCTAGTAGATAAACTCGAATAAACCAATCACAATAGACCCTGTAATGCTAAGAAGCGCGAACACAGGCACTAGATGCAATTAATCATTTGCAAAATTTGGTGTGATTATATGTAGCTAGGTTAGTGACAGTGTTAGTTTGGATAGATGCAATTTATCTACTATCAATTTACGAGATAGATGGACATAAGTGATAGGTTAGTGGGATCCTTTAAAATAGCGAAGTCACTAATGGGTCCTCTAATCTTCCACTTGTTTCTATCTATCTATTTCCCTTGAATGGACAGTAAATAGAAGCTAGATAAATGGAGCAAATCTTAGTAGACCTAGACATGCTTTCAAATGAAGTGTTGGCGCTAAATAGGTTTTATGTGTGTACAAACTTGAGTGGTCAATCTTATTGTGAGTAAAATTTAGGATGTGACGTGTGAGTATAGTAAATTGATTTGAAAATAAACGATGGCTTTATTTCAATATTTTTGTTGGAGGAGGCTGGATATTTCAATATGATTGGTTATGTATGTATATTTGTATAATATAAATCTTTAATTTGTTCATCAATGGTTATATTACCCACTTTACATTTTAAATGGATTTGCTTACTTAGTAGAAGTCAGATCATAGAATTTAACTTGAAATGGAAATGTGTTGTTTATTTTTCAATGAAAATGTTCAATTATAATCAACAGTTTCAAAATCTAATAATTTTATGTGTGTTTTATAAGATTTCAATTGTATATTATAAACAGGGGCCCTTGGAATGATATTTTAAAATTGACTCCCTTGTGGTTAAGAACATTAAAATCTCATTTGTTTGTATAATCAAATGGTATTCTGTTTGTATTTTCCGCATATAAACTTTATTAGTTGTCATATATTGAGACAGTGCTGGATGAGTAGTTTGTGTTGGTTTGTTTCATTGGTTTATAGTTCTGGTCTTTTTTTTCCTGCAATCGAATCTATGTATGCCAGAATTTGTTACTCGTGTTTGTTCATTACTTTTGCTATAAACAGATTTATTAATGCTATTTATTTTTGCAGGCTACATTTGACTGTCTGATGAAGACTTATGGTTTCTTGACACCTGAATTCTGGAAGGAGACTCGCTTCAGTAAATCTCCATTCCAAGAGTACACTGATATGTTGGCAAAGCCCACAACAAAGGCCTTGATCTTGGAGGAGGAAAGGGCGGAAGCTTAA
Protein sequence

>MS.gene83062.t1

MADRGATGDRAGFGRGFGDRGRGGRGGDRGRGGRRRGAGRREEEEKWVPVTKLGRLVKEGKIRSLEQIYLHSLPIKEHQIIDTLVGPTLKDEVMKIMPVQKQTRAGQRTRFKAFVVVGDNNGHVGLGVKCSKEVATAIRGAIILAKLSVIPVRRGYWGNKIGKPHTVPCKVTGKCGSVTVRMVPAPRGSGIVAARVPKKVLQFAGIDDVFTSSRGSTKTLGNFVKATFDCLMKTYGFLTPEFWKETRFSKSPFQEYTDMLAKPTTKALILEEERAEA