Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene26518.t1 | XP_024637292.1 | 92.6 | 148 | 11 | 0 | 1 | 148 | 1 | 148 | 1.40E-68 | 268.9 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene26518.t1 | A0A396I9D0 | 92.6 | 148 | 11 | 0 | 1 | 148 | 1 | 148 | 1.0e-68 | 268.9 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049222 | MS.gene26518 | -0.816981 | 4.18E-52 | -1.69E-46 |
| MS.gene049320 | MS.gene26518 | -0.807268 | 5.49E-50 | -1.69E-46 |
| MS.gene04932 | MS.gene26518 | 0.815903 | 7.28E-52 | -1.69E-46 |
| MS.gene049549 | MS.gene26518 | 0.803229 | 3.85E-49 | -1.69E-46 |
| MS.gene049633 | MS.gene26518 | -0.802349 | 5.85E-49 | -1.69E-46 |
| MS.gene050170 | MS.gene26518 | -0.825529 | 4.46E-54 | -1.69E-46 |
| MS.gene050504 | MS.gene26518 | -0.806061 | 9.88E-50 | -1.69E-46 |
| MS.gene050577 | MS.gene26518 | -0.810199 | 1.30E-50 | -1.69E-46 |
| MS.gene050612 | MS.gene26518 | -0.80754 | 4.81E-50 | -1.69E-46 |
| MS.gene050675 | MS.gene26518 | -0.804522 | 2.07E-49 | -1.69E-46 |
| MS.gene050699 | MS.gene26518 | -0.803039 | 4.22E-49 | -1.69E-46 |
| MS.gene050868 | MS.gene26518 | -0.821519 | 3.87E-53 | -1.69E-46 |
| MS.gene050871 | MS.gene26518 | -0.81853 | 1.87E-52 | -1.69E-46 |
| MS.gene050904 | MS.gene26518 | -0.822774 | 1.98E-53 | -1.69E-46 |
| MS.gene051138 | MS.gene26518 | 0.801967 | 7.01E-49 | -1.69E-46 |
| MS.gene051858 | MS.gene26518 | -0.807647 | 4.56E-50 | -1.69E-46 |
| MS.gene052211 | MS.gene26518 | 0.813892 | 2.03E-51 | -1.69E-46 |
| MS.gene05244 | MS.gene26518 | 0.802867 | 4.58E-49 | -1.69E-46 |
| MS.gene052606 | MS.gene26518 | 0.81428 | 1.67E-51 | -1.69E-46 |
| MS.gene053420 | MS.gene26518 | 0.811305 | 7.48E-51 | -1.69E-46 |
| MS.gene053479 | MS.gene26518 | -0.82639 | 2.79E-54 | -1.69E-46 |
| MS.gene053480 | MS.gene26518 | -0.813387 | 2.63E-51 | -1.69E-46 |
| MS.gene053824 | MS.gene26518 | -0.812023 | 5.22E-51 | -1.69E-46 |
| MS.gene054344 | MS.gene26518 | -0.802179 | 6.34E-49 | -1.69E-46 |
| MS.gene05470 | MS.gene26518 | -0.824812 | 6.59E-54 | -1.69E-46 |
| MS.gene055362 | MS.gene26518 | -0.807687 | 4.48E-50 | -1.69E-46 |
| MS.gene055502 | MS.gene26518 | -0.81175 | 5.99E-51 | -1.69E-46 |
| MS.gene055577 | MS.gene26518 | -0.802732 | 4.88E-49 | -1.69E-46 |
| MS.gene05589 | MS.gene26518 | -0.821312 | 4.32E-53 | -1.69E-46 |
| MS.gene056387 | MS.gene26518 | -0.822228 | 2.65E-53 | -1.69E-46 |
| MS.gene056504 | MS.gene26518 | -0.822488 | 2.31E-53 | -1.69E-46 |
| MS.gene056590 | MS.gene26518 | 0.824045 | 9.99E-54 | -1.69E-46 |
| MS.gene056591 | MS.gene26518 | 0.824955 | 6.10E-54 | -1.69E-46 |
| MS.gene056688 | MS.gene26518 | 0.801513 | 8.69E-49 | -1.69E-46 |
| MS.gene057831 | MS.gene26518 | -0.800168 | 1.64E-48 | -1.69E-46 |
| MS.gene058334 | MS.gene26518 | 0.814559 | 1.45E-51 | -1.69E-46 |
| MS.gene05837 | MS.gene26518 | -0.806407 | 8.35E-50 | -1.69E-46 |
| MS.gene058419 | MS.gene26518 | -0.816446 | 5.51E-52 | -1.69E-46 |
| MS.gene058452 | MS.gene26518 | 0.811455 | 6.94E-51 | -1.69E-46 |
| MS.gene058603 | MS.gene26518 | 0.807575 | 4.73E-50 | -1.69E-46 |
| MS.gene058838 | MS.gene26518 | -0.802415 | 5.67E-49 | -1.69E-46 |
| MS.gene059677 | MS.gene26518 | -0.812852 | 3.44E-51 | -1.69E-46 |
| MS.gene059865 | MS.gene26518 | -0.809765 | 1.61E-50 | -1.69E-46 |
| MS.gene059955 | MS.gene26518 | 0.804579 | 2.02E-49 | -1.69E-46 |
| MS.gene06000 | MS.gene26518 | -0.807816 | 4.20E-50 | -1.69E-46 |
| MS.gene060593 | MS.gene26518 | 0.801052 | 1.08E-48 | -1.69E-46 |
| MS.gene060762 | MS.gene26518 | -0.827579 | 1.45E-54 | -1.69E-46 |
| MS.gene060778 | MS.gene26518 | -0.803242 | 3.83E-49 | -1.69E-46 |
| MS.gene06077 | MS.gene26518 | -0.817173 | 3.78E-52 | -1.69E-46 |
| MS.gene060982 | MS.gene26518 | -0.800948 | 1.13E-48 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene26518.t1 | MTR_4g086340 | 92.537 | 134 | 10 | 0 | 1 | 134 | 1 | 134 | 4.52e-90 | 258 |
| MS.gene26518.t1 | MTR_5g017580 | 43.871 | 155 | 64 | 6 | 1 | 144 | 22 | 164 | 3.22e-23 | 89.7 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene26518.t1 | AT4G34419 | 50.000 | 114 | 40 | 8 | 2 | 105 | 17 | 123 | 6.98e-21 | 82.8 |
| MS.gene26518.t1 | AT3G19200 | 45.714 | 105 | 46 | 4 | 1 | 96 | 15 | 117 | 8.77e-16 | 69.7 |
Find 27 sgRNAs with CRISPR-Local
Find 44 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CTTAAACTCTTTGGCCATTT+TGG | 0.123678 | 4.1:+23456656 | None:intergenic |
| ATCTCTTATAGTCACCTTCT+TGG | 0.264844 | 4.1:+23456703 | None:intergenic |
| TTCCACCTGCTTAAACTCTT+TGG | 0.291424 | 4.1:+23456647 | None:intergenic |
| TAGATGAACCTGATCTCTTT+AGG | 0.311116 | 4.1:+23456612 | None:intergenic |
| TAACAACATGCCCTTGAAAA+AGG | 0.323318 | 4.1:+23456745 | None:intergenic |
| ATGATGATGAATCACCAAAA+TGG | 0.355007 | 4.1:-23456670 | MS.gene26518:CDS |
| TTGGCTAACAACAAAATCAT+TGG | 0.372042 | 4.1:+23456865 | None:intergenic |
| AAATGTTAACAAAGACAATA+TGG | 0.372168 | 4.1:-23456513 | MS.gene26518:intron |
| TTTCATGTTTGATTGCTTGT+TGG | 0.384433 | 4.1:+23456846 | None:intergenic |
| CAGAGAAGCTCCTGTCTCTT+CGG | 0.436882 | 4.1:-23456816 | MS.gene26518:CDS |
| TCATTGGAGAAGGAAATTCT+TGG | 0.437332 | 4.1:+23456881 | None:intergenic |
| GTTTCTCCTTGTCCAAAACT+AGG | 0.461812 | 4.1:+23456536 | None:intergenic |
| TCACAGGAGTTGTTCTATGA+AGG | 0.474590 | 4.1:-23456122 | MS.gene26518:CDS |
| AGATTAGGCCTAAAGAGATC+AGG | 0.493470 | 4.1:-23456620 | MS.gene26518:CDS |
| AATTCGAAATCCGAAGAGAC+AGG | 0.510616 | 4.1:+23456806 | None:intergenic |
| GAAGGAAATTCTTGGATCCA+TGG | 0.520048 | 4.1:+23456889 | None:intergenic |
| AGCAGGTGGAAAGAGAGATT+AGG | 0.538474 | 4.1:-23456635 | MS.gene26518:CDS |
| CATTTCTTATCAGTTATCAC+AGG | 0.544334 | 4.1:-23456138 | MS.gene26518:intron |
| AACAACAAAATCATTGGAGA+AGG | 0.557389 | 4.1:+23456871 | None:intergenic |
| TTGTTACCTCTCAGTAGTGA+AGG | 0.558838 | 4.1:-23456728 | MS.gene26518:CDS |
| GGCCAAAGAGTTTAAGCAGG+TGG | 0.585556 | 4.1:-23456649 | MS.gene26518:CDS |
| AGGTTCATCTAAGAAAGATA+AGG | 0.599802 | 4.1:-23456600 | MS.gene26518:CDS |
| GAAGGAGGTGTAAGTTGCAG+AGG | 0.611394 | 4.1:-23456104 | MS.gene26518:CDS |
| AATGGCCAAAGAGTTTAAGC+AGG | 0.611976 | 4.1:-23456652 | MS.gene26518:CDS |
| CAGGAGTTGTTCTATGAAGG+AGG | 0.621435 | 4.1:-23456119 | MS.gene26518:CDS |
| CAGTAGTGAAGGTTCCAAGA+AGG | 0.627710 | 4.1:-23456717 | MS.gene26518:CDS |
| TTGGAACCTTCACTACTGAG+AGG | 0.685927 | 4.1:+23456722 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AAATGTTAACAAAGACAATA+TGG | - | chr4.1:23456471-23456490 | MS.gene26518:intron | 20.0% |
| !! | GTTTATCATATAAATGCTTA+TGG | - | chr4.1:23456601-23456620 | MS.gene26518:CDS | 20.0% |
| !! | TATAAATGCTTATGGAAATA+AGG | - | chr4.1:23456609-23456628 | MS.gene26518:CDS | 20.0% |
| !! | TCAAATAATTCAATCTAAAC+AGG | - | chr4.1:23456768-23456787 | MS.gene26518:CDS | 20.0% |
| ! | CTTGTTCATATAAACTATCT+TGG | - | chr4.1:23456659-23456678 | MS.gene26518:CDS | 25.0% |
| ! | TGAAAAAGAATTAGATGAGA+TGG | + | chr4.1:23456513-23456532 | None:intergenic | 25.0% |
| !! | GGTGAAACTATTTTCATATA+AGG | - | chr4.1:23456630-23456649 | MS.gene26518:CDS | 25.0% |
| !!! | AAATGCTACTTTAAGTACTA+AGG | + | chr4.1:23456795-23456814 | None:intergenic | 25.0% |
| AACAACAAAATCATTGGAGA+AGG | + | chr4.1:23456116-23456135 | None:intergenic | 30.0% | |
| AGGTTCATCTAAGAAAGATA+AGG | - | chr4.1:23456384-23456403 | MS.gene26518:intron | 30.0% | |
| CATTTCTTATCAGTTATCAC+AGG | - | chr4.1:23456846-23456865 | MS.gene26518:CDS | 30.0% | |
| CTGTGATAACTGATAAGAAA+TGG | + | chr4.1:23456848-23456867 | None:intergenic | 30.0% | |
| TTGGCTAACAACAAAATCAT+TGG | + | chr4.1:23456122-23456141 | None:intergenic | 30.0% | |
| TTTCATGTTTGATTGCTTGT+TGG | + | chr4.1:23456141-23456160 | None:intergenic | 30.0% | |
| ! | ATGATGATGAATCACCAAAA+TGG | - | chr4.1:23456314-23456333 | MS.gene26518:intron | 30.0% |
| AAACTATCTTGGAGAGCTTA+TGG | - | chr4.1:23456670-23456689 | MS.gene26518:CDS | 35.0% | |
| ACGTTTGTGATATACACACT+TGG | + | chr4.1:23456583-23456602 | None:intergenic | 35.0% | |
| ATCTCTTATAGTCACCTTCT+TGG | + | chr4.1:23456284-23456303 | None:intergenic | 35.0% | |
| TAACAACATGCCCTTGAAAA+AGG | + | chr4.1:23456242-23456261 | None:intergenic | 35.0% | |
| TAGATGAACCTGATCTCTTT+AGG | + | chr4.1:23456375-23456394 | None:intergenic | 35.0% | |
| TCATTGGAGAAGGAAATTCT+TGG | + | chr4.1:23456106-23456125 | None:intergenic | 35.0% | |
| TTGGAGAGCTTATGGAAATA+AGG | - | chr4.1:23456678-23456697 | MS.gene26518:CDS | 35.0% | |
| ! | CTTAAACTCTTTGGCCATTT+TGG | + | chr4.1:23456331-23456350 | None:intergenic | 35.0% |
| !!! | AACCAAAAAGTGCCTAGTTT+TGG | - | chr4.1:23456436-23456455 | MS.gene26518:intron | 35.0% |
| AATGGCCAAAGAGTTTAAGC+AGG | - | chr4.1:23456332-23456351 | MS.gene26518:intron | 40.0% | |
| AATTCGAAATCCGAAGAGAC+AGG | + | chr4.1:23456181-23456200 | None:intergenic | 40.0% | |
| AGATTAGGCCTAAAGAGATC+AGG | - | chr4.1:23456364-23456383 | MS.gene26518:intron | 40.0% | |
| GTTTCTCCTTGTCCAAAACT+AGG | + | chr4.1:23456451-23456470 | None:intergenic | 40.0% | |
| TCACAGGAGTTGTTCTATGA+AGG | - | chr4.1:23456862-23456881 | MS.gene26518:CDS | 40.0% | |
| TTCCACCTGCTTAAACTCTT+TGG | + | chr4.1:23456340-23456359 | None:intergenic | 40.0% | |
| TTGTTACCTCTCAGTAGTGA+AGG | - | chr4.1:23456256-23456275 | MS.gene26518:intron | 40.0% | |
| ! | GTCCAAAACTAGGCACTTTT+TGG | + | chr4.1:23456441-23456460 | None:intergenic | 40.0% |
| !! | AGCAGATCAAGCCTTTTTCA+AGG | - | chr4.1:23456228-23456247 | MS.gene26518:intron | 40.0% |
| !!! | AAAGTGCCTAGTTTTGGACA+AGG | - | chr4.1:23456442-23456461 | MS.gene26518:intron | 40.0% |
| !!! | GCAGATCAAGCCTTTTTCAA+GGG | - | chr4.1:23456229-23456248 | MS.gene26518:intron | 40.0% |
| AGCAGGTGGAAAGAGAGATT+AGG | - | chr4.1:23456349-23456368 | MS.gene26518:intron | 45.0% | |
| CAGGAGTTGTTCTATGAAGG+AGG | - | chr4.1:23456865-23456884 | MS.gene26518:CDS | 45.0% | |
| CAGTAGTGAAGGTTCCAAGA+AGG | - | chr4.1:23456267-23456286 | MS.gene26518:intron | 45.0% | |
| TTGGAACCTTCACTACTGAG+AGG | + | chr4.1:23456265-23456284 | None:intergenic | 45.0% | |
| CAGAGAAGCTCCTGTCTCTT+CGG | - | chr4.1:23456168-23456187 | MS.gene26518:intron | 50.0% | |
| GGCCAAAGAGTTTAAGCAGG+TGG | - | chr4.1:23456335-23456354 | MS.gene26518:intron | 50.0% | |
| ! | GAAGGAGGTGTAAGTTGCAG+AGG | - | chr4.1:23456880-23456899 | MS.gene26518:CDS | 50.0% |
| CGCTCTCAGACAGTCTCTCA+GGG | - | chr4.1:23456726-23456745 | MS.gene26518:CDS | 55.0% | |
| GCGCTCTCAGACAGTCTCTC+AGG | - | chr4.1:23456725-23456744 | MS.gene26518:CDS | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr4.1 | gene | 23456097 | 23456909 | 23456097 | ID=MS.gene26518 |
| chr4.1 | mRNA | 23456097 | 23456909 | 23456097 | ID=MS.gene26518.t1;Parent=MS.gene26518 |
| chr4.1 | exon | 23456514 | 23456909 | 23456514 | ID=MS.gene26518.t1.exon1;Parent=MS.gene26518.t1 |
| chr4.1 | CDS | 23456514 | 23456909 | 23456514 | ID=cds.MS.gene26518.t1;Parent=MS.gene26518.t1 |
| chr4.1 | exon | 23456097 | 23456147 | 23456097 | ID=MS.gene26518.t1.exon2;Parent=MS.gene26518.t1 |
| chr4.1 | CDS | 23456097 | 23456147 | 23456097 | ID=cds.MS.gene26518.t1;Parent=MS.gene26518.t1 |
| Gene Sequence |
| Protein sequence |
