Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene27254.t1 | XP_013466138.1 | 95 | 378 | 19 | 0 | 1 | 378 | 1 | 378 | 1.30E-206 | 728.8 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene27254.t1 | Q9AR07 | 52.8 | 390 | 167 | 5 | 1 | 376 | 1 | 387 | 1.4e-103 | 377.9 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene27254.t1 | A0A072VED2 | 95.0 | 378 | 19 | 0 | 1 | 378 | 1 | 378 | 9.4e-207 | 728.8 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049356 | MS.gene27254 | 0.811751 | 5.98E-51 | -1.69E-46 |
| MS.gene049369 | MS.gene27254 | 0.810145 | 1.33E-50 | -1.69E-46 |
| MS.gene049396 | MS.gene27254 | 0.869796 | 2.37E-66 | -1.69E-46 |
| MS.gene049552 | MS.gene27254 | 0.806602 | 7.60E-50 | -1.69E-46 |
| MS.gene049656 | MS.gene27254 | 0.815268 | 1.01E-51 | -1.69E-46 |
| MS.gene050170 | MS.gene27254 | 0.805446 | 1.33E-49 | -1.69E-46 |
| MS.gene050499 | MS.gene27254 | 0.816006 | 6.91E-52 | -1.69E-46 |
| MS.gene050502 | MS.gene27254 | 0.871945 | 4.64E-67 | -1.69E-46 |
| MS.gene050747 | MS.gene27254 | 0.805246 | 1.46E-49 | -1.69E-46 |
| MS.gene050772 | MS.gene27254 | 0.806334 | 8.65E-50 | -1.69E-46 |
| MS.gene050950 | MS.gene27254 | 0.808214 | 3.46E-50 | -1.69E-46 |
| MS.gene051509 | MS.gene27254 | 0.808615 | 2.84E-50 | -1.69E-46 |
| MS.gene051941 | MS.gene27254 | 0.812705 | 3.71E-51 | -1.69E-46 |
| MS.gene052346 | MS.gene27254 | 0.872627 | 2.75E-67 | -1.69E-46 |
| MS.gene052414 | MS.gene27254 | 0.804746 | 1.86E-49 | -1.69E-46 |
| MS.gene053318 | MS.gene27254 | 0.823361 | 1.44E-53 | -1.69E-46 |
| MS.gene053436 | MS.gene27254 | 0.807421 | 5.10E-50 | -1.69E-46 |
| MS.gene053449 | MS.gene27254 | 0.800443 | 1.44E-48 | -1.69E-46 |
| MS.gene053505 | MS.gene27254 | 0.838329 | 3.07E-57 | -1.69E-46 |
| MS.gene053574 | MS.gene27254 | 0.843191 | 1.63E-58 | -1.69E-46 |
| MS.gene053626 | MS.gene27254 | 0.842554 | 2.41E-58 | -1.69E-46 |
| MS.gene053824 | MS.gene27254 | 0.803642 | 3.16E-49 | -1.69E-46 |
| MS.gene053912 | MS.gene27254 | 0.808553 | 2.93E-50 | -1.69E-46 |
| MS.gene05494 | MS.gene27254 | 0.832577 | 8.73E-56 | -1.69E-46 |
| MS.gene05505 | MS.gene27254 | 0.806494 | 8.01E-50 | -1.69E-46 |
| MS.gene055722 | MS.gene27254 | 0.81168 | 6.20E-51 | -1.69E-46 |
| MS.gene055741 | MS.gene27254 | 0.832254 | 1.05E-55 | -1.69E-46 |
| MS.gene055744 | MS.gene27254 | 0.828139 | 1.06E-54 | -1.69E-46 |
| MS.gene055909 | MS.gene27254 | 0.871541 | 6.32E-67 | -1.69E-46 |
| MS.gene055911 | MS.gene27254 | 0.86378 | 1.95E-64 | -1.69E-46 |
| MS.gene05601 | MS.gene27254 | 0.80204 | 6.77E-49 | -1.69E-46 |
| MS.gene056166 | MS.gene27254 | 0.815844 | 7.51E-52 | -1.69E-46 |
| MS.gene056346 | MS.gene27254 | 0.827884 | 1.22E-54 | -1.69E-46 |
| MS.gene056422 | MS.gene27254 | 0.838256 | 3.20E-57 | -1.69E-46 |
| MS.gene056448 | MS.gene27254 | 0.827713 | 1.34E-54 | -1.69E-46 |
| MS.gene056511 | MS.gene27254 | 0.827613 | 1.42E-54 | -1.69E-46 |
| MS.gene05666 | MS.gene27254 | 0.818722 | 1.69E-52 | -1.69E-46 |
| MS.gene056878 | MS.gene27254 | 0.802222 | 6.22E-49 | -1.69E-46 |
| MS.gene057019 | MS.gene27254 | 0.816996 | 4.15E-52 | -1.69E-46 |
| MS.gene057076 | MS.gene27254 | 0.806387 | 8.43E-50 | -1.69E-46 |
| MS.gene057734 | MS.gene27254 | 0.836517 | 8.93E-57 | -1.69E-46 |
| MS.gene05787 | MS.gene27254 | 0.80494 | 1.70E-49 | -1.69E-46 |
| MS.gene058075 | MS.gene27254 | 0.80674 | 7.10E-50 | -1.69E-46 |
| MS.gene058120 | MS.gene27254 | 0.820371 | 7.11E-53 | -1.69E-46 |
| MS.gene058948 | MS.gene27254 | 0.811269 | 7.62E-51 | -1.69E-46 |
| MS.gene059367 | MS.gene27254 | 0.810857 | 9.36E-51 | -1.69E-46 |
| MS.gene059368 | MS.gene27254 | 0.831242 | 1.87E-55 | -1.69E-46 |
| MS.gene059517 | MS.gene27254 | 0.836211 | 1.07E-56 | -1.69E-46 |
| MS.gene059667 | MS.gene27254 | 0.809119 | 2.22E-50 | -1.69E-46 |
| MS.gene059674 | MS.gene27254 | 0.823603 | 1.27E-53 | -1.69E-46 |
| MS.gene059680 | MS.gene27254 | 0.811666 | 6.25E-51 | -1.69E-46 |
| MS.gene059711 | MS.gene27254 | 0.803205 | 3.90E-49 | -1.69E-46 |
| MS.gene059807 | MS.gene27254 | 0.804531 | 2.07E-49 | -1.69E-46 |
| MS.gene060372 | MS.gene27254 | 0.837929 | 3.89E-57 | -1.69E-46 |
| MS.gene060482 | MS.gene27254 | 0.82318 | 1.59E-53 | -1.69E-46 |
| MS.gene060778 | MS.gene27254 | 0.81572 | 8.00E-52 | -1.69E-46 |
| MS.gene060837 | MS.gene27254 | 0.838427 | 2.90E-57 | -1.69E-46 |
| MS.gene06097 | MS.gene27254 | 0.854373 | 1.29E-61 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene27254.t1 | MTR_1g022465 | 94.974 | 378 | 19 | 0 | 1 | 378 | 1 | 378 | 0.0 | 736 |
| MS.gene27254.t1 | MTR_1g022455 | 89.710 | 379 | 38 | 1 | 1 | 378 | 1 | 379 | 0.0 | 681 |
| MS.gene27254.t1 | MTR_8g076900 | 46.684 | 377 | 185 | 4 | 1 | 377 | 1 | 361 | 3.77e-114 | 337 |
| MS.gene27254.t1 | MTR_5g020940 | 45.455 | 385 | 187 | 8 | 1 | 377 | 11 | 380 | 2.18e-112 | 333 |
| MS.gene27254.t1 | MTR_8g077040 | 42.969 | 384 | 195 | 7 | 1 | 376 | 1 | 368 | 2.77e-108 | 322 |
| MS.gene27254.t1 | MTR_6g056070 | 43.386 | 378 | 191 | 7 | 1 | 377 | 1 | 356 | 3.61e-105 | 314 |
| MS.gene27254.t1 | MTR_3g467270 | 42.408 | 382 | 196 | 8 | 4 | 377 | 3 | 368 | 4.93e-104 | 311 |
| MS.gene27254.t1 | MTR_4g018820 | 42.706 | 377 | 197 | 6 | 1 | 377 | 1 | 358 | 7.16e-103 | 308 |
| MS.gene27254.t1 | MTR_8g020690 | 43.008 | 379 | 194 | 8 | 1 | 377 | 1 | 359 | 1.07e-98 | 297 |
| MS.gene27254.t1 | MTR_3g117060 | 41.885 | 382 | 203 | 6 | 1 | 377 | 1 | 368 | 5.96e-97 | 293 |
| MS.gene27254.t1 | MTR_3g467560 | 46.419 | 377 | 184 | 8 | 3 | 376 | 26 | 387 | 2.08e-96 | 293 |
| MS.gene27254.t1 | MTR_7g084620 | 43.194 | 382 | 192 | 7 | 1 | 376 | 1 | 363 | 3.43e-94 | 286 |
| MS.gene27254.t1 | MTR_8g020700 | 40.789 | 380 | 198 | 7 | 1 | 377 | 1 | 356 | 8.61e-94 | 285 |
| MS.gene27254.t1 | MTR_5g046110 | 41.379 | 377 | 203 | 5 | 1 | 376 | 61 | 420 | 3.51e-93 | 285 |
| MS.gene27254.t1 | MTR_7g084350 | 40.533 | 375 | 204 | 7 | 6 | 376 | 4 | 363 | 3.78e-85 | 263 |
| MS.gene27254.t1 | MTR_8g076900 | 49.807 | 259 | 114 | 4 | 1 | 259 | 1 | 243 | 8.19e-79 | 243 |
| MS.gene27254.t1 | MTR_7g084330 | 40.616 | 357 | 174 | 8 | 25 | 376 | 8 | 331 | 1.15e-71 | 227 |
| MS.gene27254.t1 | MTR_8g020700 | 45.000 | 260 | 126 | 5 | 1 | 259 | 1 | 244 | 2.92e-68 | 216 |
| MS.gene27254.t1 | MTR_3g054330 | 35.264 | 397 | 211 | 13 | 1 | 375 | 10 | 382 | 2.65e-57 | 191 |
| MS.gene27254.t1 | MTR_5g093900 | 31.726 | 394 | 231 | 11 | 1 | 374 | 13 | 388 | 7.09e-55 | 186 |
| MS.gene27254.t1 | MTR_3g052700 | 33.060 | 366 | 213 | 10 | 1 | 351 | 13 | 361 | 2.92e-53 | 181 |
| MS.gene27254.t1 | MTR_1g108660 | 33.232 | 328 | 188 | 6 | 54 | 378 | 39 | 338 | 1.20e-52 | 178 |
| MS.gene27254.t1 | MTR_8g077030 | 40.851 | 235 | 101 | 5 | 1 | 235 | 1 | 197 | 1.68e-46 | 166 |
| MS.gene27254.t1 | MTR_7g084340 | 41.176 | 119 | 66 | 1 | 262 | 376 | 11 | 129 | 1.17e-20 | 87.4 |
| MS.gene27254.t1 | MTR_6g015620 | 40.833 | 120 | 66 | 1 | 263 | 377 | 18 | 137 | 3.16e-19 | 83.6 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene27254.t1 | AT1G19640 | 52.821 | 390 | 167 | 5 | 1 | 376 | 1 | 387 | 3.83e-126 | 369 |
| MS.gene27254.t1 | AT4G36470 | 40.751 | 373 | 207 | 5 | 7 | 377 | 10 | 370 | 6.75e-94 | 286 |
| MS.gene27254.t1 | AT5G66430 | 42.819 | 376 | 186 | 7 | 9 | 377 | 1 | 354 | 4.52e-92 | 280 |
| MS.gene27254.t1 | AT5G04380 | 43.386 | 378 | 189 | 8 | 4 | 377 | 33 | 389 | 6.46e-90 | 276 |
| MS.gene27254.t1 | AT3G11480 | 41.867 | 375 | 196 | 6 | 4 | 377 | 26 | 379 | 3.20e-89 | 274 |
| MS.gene27254.t1 | AT5G04380 | 42.593 | 378 | 185 | 9 | 4 | 377 | 29 | 378 | 1.62e-85 | 264 |
| MS.gene27254.t1 | AT5G04380 | 45.260 | 327 | 156 | 7 | 55 | 377 | 11 | 318 | 1.67e-83 | 257 |
| MS.gene27254.t1 | AT5G04370 | 40.576 | 382 | 194 | 7 | 4 | 378 | 36 | 391 | 3.02e-83 | 259 |
| MS.gene27254.t1 | AT5G04370 | 40.992 | 383 | 191 | 8 | 4 | 378 | 55 | 410 | 6.34e-83 | 259 |
| MS.gene27254.t1 | AT5G04370 | 40.576 | 382 | 194 | 7 | 4 | 378 | 41 | 396 | 7.10e-83 | 258 |
| MS.gene27254.t1 | AT5G38020 | 39.535 | 387 | 198 | 9 | 6 | 377 | 3 | 368 | 6.14e-82 | 255 |
| MS.gene27254.t1 | AT5G38020 | 40.828 | 338 | 166 | 8 | 55 | 377 | 11 | 329 | 2.65e-76 | 239 |
| MS.gene27254.t1 | AT3G21950 | 37.662 | 385 | 208 | 8 | 6 | 377 | 3 | 368 | 3.75e-73 | 232 |
| MS.gene27254.t1 | AT2G14060 | 37.500 | 384 | 210 | 8 | 4 | 377 | 9 | 372 | 2.19e-68 | 220 |
| MS.gene27254.t1 | AT3G21950 | 34.772 | 417 | 208 | 9 | 6 | 377 | 3 | 400 | 2.12e-66 | 216 |
| MS.gene27254.t1 | AT1G68040 | 34.085 | 355 | 206 | 8 | 6 | 356 | 10 | 340 | 2.49e-54 | 183 |
| MS.gene27254.t1 | AT5G38100 | 32.773 | 357 | 207 | 7 | 9 | 359 | 10 | 339 | 7.54e-53 | 179 |
| MS.gene27254.t1 | AT1G15125 | 33.811 | 349 | 199 | 7 | 9 | 353 | 1 | 321 | 2.02e-52 | 178 |
| MS.gene27254.t1 | AT5G55250 | 31.472 | 394 | 227 | 13 | 1 | 374 | 13 | 383 | 2.02e-52 | 179 |
| MS.gene27254.t1 | AT5G37970 | 31.266 | 387 | 222 | 10 | 5 | 377 | 6 | 362 | 1.62e-51 | 176 |
| MS.gene27254.t1 | AT5G38100 | 32.456 | 342 | 198 | 7 | 24 | 359 | 2 | 316 | 4.35e-50 | 171 |
| MS.gene27254.t1 | AT5G55250 | 32.530 | 332 | 190 | 10 | 59 | 374 | 32 | 345 | 1.20e-49 | 171 |
| MS.gene27254.t1 | AT5G37990 | 30.570 | 386 | 226 | 9 | 5 | 377 | 6 | 362 | 1.56e-49 | 171 |
| MS.gene27254.t1 | AT2G14060 | 40.244 | 246 | 127 | 5 | 4 | 248 | 9 | 235 | 7.67e-49 | 166 |
| MS.gene27254.t1 | AT5G38780 | 29.670 | 364 | 211 | 8 | 9 | 359 | 10 | 341 | 1.64e-44 | 157 |
| MS.gene27254.t1 | AT4G26420 | 30.579 | 363 | 216 | 9 | 6 | 353 | 10 | 351 | 9.69e-43 | 153 |
| MS.gene27254.t1 | AT5G38780 | 30.476 | 315 | 175 | 7 | 57 | 359 | 10 | 292 | 4.45e-42 | 150 |
| MS.gene27254.t1 | AT5G56300 | 27.793 | 367 | 236 | 8 | 3 | 356 | 14 | 364 | 5.98e-37 | 138 |
| MS.gene27254.t1 | AT3G44840 | 28.329 | 353 | 202 | 8 | 9 | 350 | 7 | 319 | 3.41e-35 | 132 |
| MS.gene27254.t1 | AT1G15125 | 34.118 | 255 | 143 | 6 | 9 | 259 | 1 | 234 | 1.20e-33 | 125 |
| MS.gene27254.t1 | AT4G26420 | 31.224 | 237 | 137 | 5 | 6 | 236 | 10 | 226 | 6.40e-31 | 119 |
| MS.gene27254.t1 | AT5G38100 | 32.589 | 224 | 131 | 4 | 9 | 229 | 10 | 216 | 7.75e-31 | 118 |
| MS.gene27254.t1 | AT1G66700 | 26.685 | 371 | 228 | 10 | 1 | 362 | 1 | 336 | 9.47e-31 | 120 |
| MS.gene27254.t1 | AT5G38100 | 32.589 | 224 | 131 | 4 | 9 | 229 | 10 | 216 | 1.01e-30 | 118 |
| MS.gene27254.t1 | AT3G44870 | 29.870 | 308 | 166 | 9 | 6 | 300 | 4 | 274 | 2.34e-30 | 120 |
| MS.gene27254.t1 | AT4G26420 | 31.197 | 234 | 135 | 5 | 9 | 236 | 1 | 214 | 3.55e-30 | 117 |
| MS.gene27254.t1 | AT1G66720 | 26.000 | 350 | 216 | 9 | 9 | 350 | 9 | 323 | 5.76e-30 | 118 |
| MS.gene27254.t1 | AT3G44860 | 30.820 | 305 | 170 | 8 | 6 | 300 | 4 | 277 | 1.21e-29 | 117 |
| MS.gene27254.t1 | AT1G66690 | 25.263 | 380 | 222 | 11 | 1 | 362 | 1 | 336 | 5.10e-26 | 107 |
| MS.gene27254.t1 | AT1G66700 | 28.936 | 235 | 136 | 6 | 1 | 229 | 1 | 210 | 2.33e-21 | 92.8 |
Find 82 sgRNAs with CRISPR-Local
Find 0 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CATGCTTGAGGTTGCATTTA+TGG | 0.055981 | 1.1:-1691517 | None:intergenic |
| GCAATTCCCATCTTCATTAT+TGG | 0.151956 | 1.1:-1691435 | None:intergenic |
| AATGACCAAATTGATGTATT+TGG | 0.221869 | 1.1:-1700477 | None:intergenic |
| CCTTATGACCATGGTTTCTA+TGG | 0.261315 | 1.1:+1699564 | MS.gene27254:CDS |
| AAGATGGGAATTGCTGATTT+AGG | 0.303966 | 1.1:+1691444 | MS.gene27254:CDS |
| CTATGCTAGAATATCATTTC+GGG | 0.312146 | 1.1:+1700384 | MS.gene27254:CDS |
| GAAATTACCCTTAGGGCATT+AGG | 0.321258 | 1.1:-1691480 | None:intergenic |
| ATATATAACATTAGTTCCTT+TGG | 0.330466 | 1.1:-1691555 | None:intergenic |
| TCTATGCTAGAATATCATTT+CGG | 0.341040 | 1.1:+1700383 | MS.gene27254:CDS |
| AGATTCAACCACAGCCCTTA+TGG | 0.342594 | 1.1:-1700363 | None:intergenic |
| TATGCAGGCCCCTGGTGGTT+TGG | 0.343156 | 1.1:+1699306 | MS.gene27254:intron |
| CAAATGATCCTCCACAAGTT+TGG | 0.347302 | 1.1:-1700441 | None:intergenic |
| ATGAAGCTTATGAAATTGAT+TGG | 0.352222 | 1.1:+1700279 | MS.gene27254:CDS |
| AATCAATTTCATAAGCTTCA+TGG | 0.357614 | 1.1:-1700277 | None:intergenic |
| TCATTTCGGGAGTCATATAA+TGG | 0.374376 | 1.1:+1700397 | MS.gene27254:CDS |
| CACCCAACTGTTGCTATCAA+TGG | 0.383419 | 1.1:+1699524 | MS.gene27254:CDS |
| TAGCCTCCATTGGCTTTCTC+AGG | 0.386982 | 1.1:+1691773 | MS.gene27254:CDS |
| CTATTTCTGAAATTACCCTT+AGG | 0.395955 | 1.1:-1691488 | None:intergenic |
| TAGGACCAAATACATCAATT+TGG | 0.396975 | 1.1:+1700472 | MS.gene27254:CDS |
| GAAACACTTCACATGAATAA+AGG | 0.402226 | 1.1:+1690790 | MS.gene27254:CDS |
| GCTGATTTAGGTTGTTCATC+AGG | 0.406185 | 1.1:+1691456 | MS.gene27254:CDS |
| CCATAGAAACCATGGTCATA+AGG | 0.419755 | 1.1:-1699564 | None:intergenic |
| TGAGTCACGTTCTCAAGAAA+TGG | 0.425270 | 1.1:+1699444 | MS.gene27254:CDS |
| AACATTAGTTCCTTTGGTGC+TGG | 0.433345 | 1.1:-1691549 | None:intergenic |
| TATGAAATTGATTGGGATGA+TGG | 0.438195 | 1.1:+1700287 | MS.gene27254:CDS |
| AACGAGTTGCAAAGACCATA+AGG | 0.441481 | 1.1:+1700348 | MS.gene27254:CDS |
| TACCTGGACAAAAGAGTTCA+TGG | 0.442526 | 1.1:-1690833 | None:intergenic |
| ACTCTTCTTCTAGCCTCCAT+TGG | 0.444348 | 1.1:+1691763 | MS.gene27254:CDS |
| TTGGAGGATGAAAGAGGAAA+AGG | 0.445045 | 1.1:+1699325 | MS.gene27254:CDS |
| TATTTCTGAAATTACCCTTA+GGG | 0.453496 | 1.1:-1691487 | None:intergenic |
| ATCAATTTCATAAGCTTCAT+GGG | 0.453860 | 1.1:-1700276 | None:intergenic |
| CTAAGTGAAGGGACTTAGTT+GGG | 0.454894 | 1.1:-1691737 | None:intergenic |
| TGATTCTATATGCAGGCCCC+TGG | 0.466597 | 1.1:+1699298 | MS.gene27254:intron |
| AGTGATGATAAGATGATAAG+TGG | 0.467732 | 1.1:+1700323 | MS.gene27254:CDS |
| ACCCAACTGTTGCTATCAAT+GGG | 0.467790 | 1.1:+1699525 | MS.gene27254:CDS |
| TCAAAACACGGTGCATAGTA+AGG | 0.468345 | 1.1:-1700206 | None:intergenic |
| ACGATGGCCAATAATGAAGA+TGG | 0.469521 | 1.1:+1691428 | MS.gene27254:CDS |
| AGCAACAGTTGGGTGACGTT+GGG | 0.481995 | 1.1:-1699516 | None:intergenic |
| TGGAGGATGAAAGAGGAAAA+GGG | 0.488214 | 1.1:+1699326 | MS.gene27254:CDS |
| CTTTCATCCTCCAAACCACC+AGG | 0.489278 | 1.1:-1699316 | None:intergenic |
| TGTTGAAGTCATTGGTGAAA+AGG | 0.512921 | 1.1:-1691584 | None:intergenic |
| GCAGGCCCCTGGTGGTTTGG+AGG | 0.513877 | 1.1:+1699309 | MS.gene27254:intron |
| TCCCATTGATAGCAACAGTT+GGG | 0.515861 | 1.1:-1699526 | None:intergenic |
| GGGTCTAGTTGAAGAAGAAA+AGG | 0.515871 | 1.1:+1700166 | MS.gene27254:intron |
| CATAGAAACCATGGTCATAA+GGG | 0.519203 | 1.1:-1699563 | None:intergenic |
| ACTAAGTGAAGGGACTTAGT+TGG | 0.520027 | 1.1:-1691738 | None:intergenic |
| TGAAGCTTATGAAATTGATT+GGG | 0.520122 | 1.1:+1700280 | MS.gene27254:CDS |
| ATGAAATTGATTGGGATGAT+GGG | 0.521002 | 1.1:+1700288 | MS.gene27254:CDS |
| TAATGGATGAGTTGTTTCAA+AGG | 0.525941 | 1.1:+1700414 | MS.gene27254:CDS |
| TTCTATATGCAGGCCCCTGG+TGG | 0.528673 | 1.1:+1699301 | MS.gene27254:intron |
| CATCAGGACCTAATGCCCTA+AGG | 0.529141 | 1.1:+1691472 | MS.gene27254:CDS |
| AGCAACAAAGAAAGCTATTG+TGG | 0.531669 | 1.1:+1691386 | MS.gene27254:CDS |
| TCAAAGGTTTGCCAAACTTG+TGG | 0.533407 | 1.1:+1700430 | MS.gene27254:CDS |
| TTCCCATTGATAGCAACAGT+TGG | 0.543485 | 1.1:-1699527 | None:intergenic |
| AGAAAAGAAAATGGAAACTG+TGG | 0.546339 | 1.1:+1690768 | None:intergenic |
| CGTTCTCAAGAAATGGTGCA+TGG | 0.550195 | 1.1:+1699451 | MS.gene27254:CDS |
| ATTCCCTGCCCATAAAAGAA+AGG | 0.561138 | 1.1:-1699486 | None:intergenic |
| GTTCTCAAGAAATGGTGCAT+GGG | 0.561345 | 1.1:+1699452 | MS.gene27254:CDS |
| CGATGGCCAATAATGAAGAT+GGG | 0.562710 | 1.1:+1691429 | MS.gene27254:CDS |
| AAGAAGAGTGCACTAAGTGA+AGG | 0.563107 | 1.1:-1691749 | None:intergenic |
| AAGGTTTGCCAAACTTGTGG+AGG | 0.571113 | 1.1:+1700433 | MS.gene27254:CDS |
| TTCTCAAGAAATGGTGCATG+GGG | 0.571897 | 1.1:+1699453 | MS.gene27254:CDS |
| TGCAAAGACCATAAGGGCTG+TGG | 0.574140 | 1.1:+1700355 | MS.gene27254:CDS |
| ATCAGGACCTAATGCCCTAA+GGG | 0.574940 | 1.1:+1691473 | MS.gene27254:CDS |
| ACGAGTTGCAAAGACCATAA+GGG | 0.576120 | 1.1:+1700349 | MS.gene27254:CDS |
| AGAAGAGTGCACTAAGTGAA+GGG | 0.581048 | 1.1:-1691748 | None:intergenic |
| AATTAACCTGAGAAAGCCAA+TGG | 0.584397 | 1.1:-1691779 | None:intergenic |
| AGAGGAAAAGGGTTGAACAA+AGG | 0.586631 | 1.1:+1699337 | MS.gene27254:CDS |
| AGCACAGTCCCTTATGACCA+TGG | 0.588105 | 1.1:+1699555 | MS.gene27254:CDS |
| TTCATCCTCCAAACCACCAG+GGG | 0.588760 | 1.1:-1699314 | None:intergenic |
| TAGCAACAGTTGGGTGACGT+TGG | 0.590620 | 1.1:-1699517 | None:intergenic |
| TGGTCGATTCAACATGCTTG+AGG | 0.595941 | 1.1:-1691529 | None:intergenic |
| GTTGAATCGACCAGCACCAA+AGG | 0.600184 | 1.1:+1691539 | MS.gene27254:CDS |
| GGTGGTTTGGAGGATGAAAG+AGG | 0.602136 | 1.1:+1699319 | MS.gene27254:CDS |
| CTTCACATGAATAAAGGAGC+TGG | 0.607314 | 1.1:+1690796 | MS.gene27254:CDS |
| TTTCATCCTCCAAACCACCA+GGG | 0.628257 | 1.1:-1699315 | None:intergenic |
| GATAATCATCATAATCACAA+TGG | 0.636262 | 1.1:+1691663 | MS.gene27254:CDS |
| TCTAGTTGAAGAAGAAAAGG+TGG | 0.646901 | 1.1:+1700169 | MS.gene27254:CDS |
| TTCTATGCTCAACAAAACGA+TGG | 0.656489 | 1.1:+1691412 | MS.gene27254:CDS |
| TAACCTGAGAAAGCCAATGG+AGG | 0.661820 | 1.1:-1691776 | None:intergenic |
| GTAACGTACCATAGAAACCA+TGG | 0.673939 | 1.1:-1699572 | None:intergenic |
| TCTCAAGAAATGGTGCATGG+GGG | 0.747092 | 1.1:+1699454 | MS.gene27254:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr1.1 | gene | 1690778 | 1700520 | 1690778 | ID=MS.gene27254 |
| chr1.1 | mRNA | 1690778 | 1700520 | 1690778 | ID=MS.gene27254.t1;Parent=MS.gene27254 |
| chr1.1 | exon | 1690778 | 1690852 | 1690778 | ID=MS.gene27254.t1.exon1;Parent=MS.gene27254.t1 |
| chr1.1 | CDS | 1690778 | 1690852 | 1690778 | ID=cds.MS.gene27254.t1;Parent=MS.gene27254.t1 |
| chr1.1 | exon | 1691360 | 1691794 | 1691360 | ID=MS.gene27254.t1.exon2;Parent=MS.gene27254.t1 |
| chr1.1 | CDS | 1691360 | 1691794 | 1691360 | ID=cds.MS.gene27254.t1;Parent=MS.gene27254.t1 |
| chr1.1 | exon | 1699313 | 1699585 | 1699313 | ID=MS.gene27254.t1.exon3;Parent=MS.gene27254.t1 |
| chr1.1 | CDS | 1699313 | 1699585 | 1699313 | ID=cds.MS.gene27254.t1;Parent=MS.gene27254.t1 |
| chr1.1 | exon | 1700167 | 1700520 | 1700167 | ID=MS.gene27254.t1.exon4;Parent=MS.gene27254.t1 |
| chr1.1 | CDS | 1700167 | 1700520 | 1700167 | ID=cds.MS.gene27254.t1;Parent=MS.gene27254.t1 |
| Gene Sequence |
| Protein sequence |
