AlfalfaGEDB | MS.gene30927

M. sativa cultivar XinJiangDaYe / MS.gene30927

KEGG: -
GO: GO:0003674 GO:0005488 GO:0005515
PFAM: PF12796.10 PF13857.9 PF13962.9

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene30927.t1	XP_003597310.1	97.6	545	12	1	1	545	1	544	1.30E-183	652.9

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene30927.t1	Q6AWW5	67.6	534	156	3	15	545	5	524	3.7e-86	320.5

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene30927.t1	G7IUA5	97.6	545	12	1	1	545	1	544	9.4e-184	652.9

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR

PPI:

Gene1	Gene2	Type
MS.gene30927	MS.gene03927	PPI
MS.gene30927	MS.gene20251	PPI
MS.gene30927	MS.gene38996	PPI
MS.gene30927	MS.gene008451	PPI
MS.gene30927	MS.gene008654	PPI
MS.gene30927	MS.gene25041	PPI
MS.gene30927	MS.gene38433	PPI
MS.gene30927	MS.gene38428	PPI
MS.gene30927	MS.gene41482	PPI
MS.gene30927	MS.gene028458	PPI
MS.gene30927	MS.gene05756	PPI
MS.gene30927	MS.gene41488	PPI
MS.gene30927	MS.gene028243	PPI
MS.gene049563	MS.gene30927	PPI
MS.gene071898	MS.gene30927	PPI
MS.gene30927	MS.gene43534	PPI
MS.gene30927	MS.gene09607	PPI
MS.gene30927	MS.gene008470	PPI
MS.gene30927	MS.gene049560	PPI
MS.gene30927	MS.gene017541	PPI
MS.gene30927	MS.gene020402	PPI
MS.gene30927	MS.gene76441	PPI
MS.gene30927	MS.gene03932	PPI
MS.gene30927	MS.gene38427	PPI
MS.gene011756	MS.gene30927	PPI
MS.gene30927	MS.gene20305	PPI
MS.gene30927	MS.gene028244	PPI
MS.gene30927	MS.gene32240	PPI
MS.gene008464	MS.gene30927	PPI
MS.gene30927	MS.gene05139	PPI
MS.gene05753	MS.gene30927	PPI
MS.gene38422	MS.gene30927	PPI
MS.gene30927	MS.gene048534	PPI
MS.gene30927	MS.gene81040	PPI
MS.gene30927	MS.gene041920	PPI
MS.gene41487	MS.gene30927	PPI
MS.gene30927	MS.gene05138	PPI
MS.gene30927	MS.gene08405	PPI
MS.gene015265	MS.gene30927	PPI
MS.gene30927	MS.gene008452	PPI
MS.gene30927	MS.gene38425	PPI
MS.gene89606	MS.gene30927	PPI
MS.gene30927	MS.gene41481	PPI
MS.gene30927	MS.gene068045	PPI
MS.gene30927	MS.gene90448	PPI
MS.gene30927	MS.gene049551	PPI
MS.gene30927	MS.gene05752	PPI
MS.gene38426	MS.gene30927	PPI
MS.gene30927	MS.gene48445	PPI
MS.gene30927	MS.gene008655	PPI
MS.gene30927	MS.gene23830	PPI
MS.gene30927	MS.gene015211	PPI
MS.gene30927	MS.gene27240	PPI
MS.gene30927	MS.gene051024	PPI
MS.gene30927	MS.gene78702	PPI
MS.gene30927	MS.gene92274	PPI
MS.gene30927	MS.gene008653	PPI
MS.gene30927	MS.gene02178	PPI
MS.gene049552	MS.gene30927	PPI
MS.gene30927	MS.gene41484	PPI
MS.gene30927	MS.gene032323	PPI
MS.gene30927	MS.gene049555	PPI
MS.gene30927	MS.gene44512	PPI
MS.gene30927	MS.gene073427	PPI
MS.gene38420	MS.gene30927	PPI
MS.gene30927	MS.gene01192	PPI
MS.gene30927	MS.gene053559	PPI
MS.gene30927	MS.gene066483	PPI
MS.gene30927	MS.gene023218	PPI
MS.gene30927	MS.gene35210	PPI
MS.gene30927	MS.gene03929	PPI
MS.gene30927	MS.gene08407	PPI
MS.gene30927	MS.gene99572	PPI
MS.gene30927	MS.gene049576	PPI
MS.gene30927	MS.gene017540	PPI
MS.gene23794	MS.gene30927	PPI
MS.gene30927	MS.gene23793	PPI
MS.gene09550	MS.gene30927	PPI
MS.gene30927	MS.gene69986	PPI
MS.gene30927	MS.gene90199	PPI
MS.gene30927	MS.gene35208	PPI
MS.gene30927	MS.gene023219	PPI
MS.gene30927	MS.gene013038	PPI
MS.gene30927	MS.gene008465	PPI
MS.gene30927	MS.gene41475	PPI
MS.gene049562	MS.gene30927	PPI
MS.gene30927	MS.gene048532	PPI
MS.gene30927	MS.gene007855	PPI
MS.gene30927	MS.gene008474	PPI
MS.gene30927	MS.gene41476	PPI
MS.gene30927	MS.gene27241	PPI

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene30927.t1	MTR_2g096190	97.798	545	11	1	1	545	1	544	0.0	1091
MS.gene30927.t1	MTR_4g062100	77.963	540	113	4	7	545	12	546	0.0	827
MS.gene30927.t1	MTR_7g100390	63.636	528	184	4	18	545	1	520	0.0	654
MS.gene30927.t1	MTR_7g117620	57.303	534	205	6	16	545	32	546	0.0	584
MS.gene30927.t1	MTR_1g115575	46.390	554	253	8	15	545	61	593	2.99e-156	459
MS.gene30927.t1	MTR_1g032100	43.893	524	269	8	36	545	19	531	7.04e-144	425
MS.gene30927.t1	MTR_8g105260	44.106	492	255	8	62	545	59	538	2.63e-135	404
MS.gene30927.t1	MTR_8g105260	30.583	206	133	4	21	222	92	291	8.45e-14	74.3
MS.gene30927.t1	MTR_7g100430	44.624	558	262	9	10	545	50	582	8.18e-135	404
MS.gene30927.t1	MTR_2g101310	25.801	593	337	14	25	543	68	631	3.97e-44	166
MS.gene30927.t1	MTR_2g101310	27.119	236	168	3	58	291	27	260	8.67e-18	87.4
MS.gene30927.t1	MTR_2g101320	24.334	563	309	14	15	475	24	571	1.49e-36	144
MS.gene30927.t1	MTR_1g069110	27.013	385	240	11	108	467	21	389	1.22e-27	116
MS.gene30927.t1	MTR_2g101310	25.995	427	232	11	25	381	68	480	1.33e-26	113
MS.gene30927.t1	MTR_2g101310	27.119	236	168	3	58	291	27	260	3.14e-17	85.1
MS.gene30927.t1	MTR_5g085480	25.370	473	298	12	41	477	28	481	5.07e-19	90.9
MS.gene30927.t1	MTR_5g085830	24.000	450	283	12	59	477	236	657	5.59e-19	91.3
MS.gene30927.t1	MTR_5g085480	25.651	499	306	13	28	477	159	641	5.94e-19	90.9
MS.gene30927.t1	MTR_8g066670	22.921	493	285	13	59	510	1064	1502	1.06e-18	90.9
MS.gene30927.t1	MTR_5g085810	24.757	412	220	12	115	477	315	685	1.42e-18	89.7
MS.gene30927.t1	MTR_3g105650	23.636	330	204	7	73	381	8	310	9.16e-17	83.2
MS.gene30927.t1	MTR_8g073260	25.387	323	168	9	107	381	13	310	4.39e-16	81.3
MS.gene30927.t1	MTR_5g043640	32.955	176	111	5	139	312	58	228	6.50e-16	78.2
MS.gene30927.t1	MTR_5g043640	30.769	182	119	6	62	241	50	226	6.28e-12	66.2
MS.gene30927.t1	MTR_7g451420	27.273	231	152	4	70	286	9	237	1.56e-15	78.2
MS.gene30927.t1	MTR_7g451420	25.287	174	128	1	101	272	5	178	3.35e-11	65.1
MS.gene30927.t1	MTR_8g080080	30.328	244	139	7	66	278	102	345	3.70e-15	79.0
MS.gene30927.t1	MTR_8g080080	23.311	296	156	6	31	260	106	396	5.70e-13	72.0
MS.gene30927.t1	MTR_5g085500	32.530	166	105	3	131	289	1	166	4.83e-15	78.2
MS.gene30927.t1	MTR_2g096760	29.339	242	138	7	58	294	5	218	5.00e-15	77.8
MS.gene30927.t1	MTR_3g085830	25.614	285	151	9	31	276	109	371	1.00e-14	77.8
MS.gene30927.t1	MTR_7g099570	30.392	204	126	6	99	291	7	205	1.08e-14	77.4
MS.gene30927.t1	MTR_6g023070	26.174	298	165	9	31	292	54	332	1.51e-14	77.0
MS.gene30927.t1	MTR_6g023070	29.688	192	125	4	96	278	45	235	9.16e-11	65.1
MS.gene30927.t1	MTR_8g072510	23.333	330	214	7	65	381	6	309	3.52e-14	75.1
MS.gene30927.t1	MTR_2g033050	26.023	342	163	12	24	276	40	380	5.99e-14	74.3
MS.gene30927.t1	MTR_8g467780	27.473	182	129	2	105	283	11	192	7.78e-14	74.3
MS.gene30927.t1	MTR_6g022830	26.846	298	174	9	31	292	106	395	8.87e-14	74.7
MS.gene30927.t1	MTR_6g022830	26.071	280	175	8	29	278	21	298	1.40e-12	70.9
MS.gene30927.t1	MTR_6g022830	27.928	222	139	6	31	237	193	408	4.10e-12	69.3
MS.gene30927.t1	MTR_1g059930	28.049	164	116	1	102	263	9	172	1.35e-13	73.2
MS.gene30927.t1	MTR_3g016210	22.785	316	210	6	100	381	5	320	2.99e-13	72.4
MS.gene30927.t1	MTR_8g062410	25.306	245	167	3	70	301	10	251	5.06e-13	71.6
MS.gene30927.t1	MTR_4g063830	27.053	207	132	5	67	257	13	216	2.01e-12	69.7
MS.gene30927.t1	MTR_6g027840	31.351	185	104	6	25	209	308	469	2.79e-12	69.7
MS.gene30927.t1	MTR_8g040930	22.781	338	206	10	120	429	10	320	7.00e-12	67.8
MS.gene30927.t1	MTR_8g041240	22.781	338	206	10	120	429	10	320	7.00e-12	67.8
MS.gene30927.t1	MTR_7g099580	26.263	198	134	4	65	251	7	203	7.03e-12	67.4
MS.gene30927.t1	MTR_1g089180	24.648	284	176	10	34	294	161	429	7.28e-12	68.2
MS.gene30927.t1	MTR_5g026550	38.983	118	63	4	132	242	26	141	7.80e-12	64.3
MS.gene30927.t1	MTR_5g026800	34.228	149	94	4	97	245	8	152	8.10e-12	66.2
MS.gene30927.t1	MTR_4g113530	28.866	194	128	5	132	320	548	736	8.86e-12	68.6
MS.gene30927.t1	MTR_8g068990	23.179	302	177	8	106	390	49	312	1.46e-11	67.0
MS.gene30927.t1	MTR_8g069880	21.552	348	225	8	105	429	43	365	2.93e-11	66.2

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene30927.t1	AT1G07710	74.812	532	132	1	14	545	14	543	0.0	769
MS.gene30927.t1	AT1G07710	74.812	532	132	1	14	545	14	543	0.0	769
MS.gene30927.t1	AT5G60070	66.667	555	168	8	1	545	1	548	0.0	694
MS.gene30927.t1	AT5G02620	61.453	537	184	4	15	545	5	524	0.0	630
MS.gene30927.t1	AT5G02620	61.453	537	184	4	15	545	5	524	0.0	630
MS.gene30927.t1	AT5G02620	61.453	537	184	4	15	545	5	524	0.0	630
MS.gene30927.t1	AT5G02620	58.929	560	184	5	15	545	5	547	0.0	619
MS.gene30927.t1	AT5G02620	58.929	560	184	5	15	545	5	547	0.0	619
MS.gene30927.t1	AT2G31820	59.160	524	203	6	24	545	148	662	0.0	598
MS.gene30927.t1	AT1G05640	58.879	535	207	8	15	545	102	627	0.0	582
MS.gene30927.t1	AT3G12360	45.138	545	262	7	10	534	45	572	1.77e-145	431
MS.gene30927.t1	AT3G12360	45.138	545	262	7	10	534	57	584	3.92e-145	431
MS.gene30927.t1	AT2G01680	43.600	500	256	5	31	514	14	503	5.56e-139	413
MS.gene30927.t1	AT3G09550	44.665	553	267	7	13	545	74	607	5.91e-137	410
MS.gene30927.t1	AT3G09550	44.665	553	267	7	13	545	74	607	5.91e-137	410
MS.gene30927.t1	AT3G09550	44.665	553	267	7	13	545	81	614	6.01e-137	410
MS.gene30927.t1	AT5G54710	28.081	495	319	15	28	510	103	572	4.72e-40	154
MS.gene30927.t1	AT5G54710	28.081	495	319	15	28	510	148	617	6.91e-40	154
MS.gene30927.t1	AT5G54710	24.528	265	180	5	28	292	19	263	9.80e-13	71.2
MS.gene30927.t1	AT1G34050	26.547	501	320	12	17	505	75	539	1.15e-38	149
MS.gene30927.t1	AT2G24600	25.347	505	319	17	28	501	72	549	1.24e-38	150
MS.gene30927.t1	AT2G24600	25.347	505	319	17	28	501	72	549	1.24e-38	150
MS.gene30927.t1	AT1G10340	24.951	513	341	14	28	516	72	564	4.18e-33	133
MS.gene30927.t1	AT1G03670	24.790	476	287	9	25	469	144	579	1.05e-32	132
MS.gene30927.t1	AT1G03670	28.571	189	128	3	101	282	72	260	1.09e-12	71.2
MS.gene30927.t1	AT1G10340	24.756	513	338	15	28	516	72	560	1.63e-30	125
MS.gene30927.t1	AT1G10340	24.180	244	156	3	105	319	41	284	8.72e-11	65.1
MS.gene30927.t1	AT4G03460	25.971	412	251	12	25	426	125	492	1.06e-27	117
MS.gene30927.t1	AT4G03460	25.971	412	251	12	25	426	157	524	1.26e-27	117
MS.gene30927.t1	AT4G03460	34.228	149	85	4	108	244	53	200	8.21e-12	68.6
MS.gene30927.t1	AT4G03470	24.808	520	293	15	23	489	149	623	5.90e-27	115
MS.gene30927.t1	AT4G03500	23.462	520	322	13	22	484	74	574	3.06e-26	113
MS.gene30927.t1	AT4G03500	23.462	520	322	13	22	484	74	574	3.06e-26	113
MS.gene30927.t1	AT4G03500	23.462	520	322	13	22	484	97	597	3.25e-26	113
MS.gene30927.t1	AT5G50140	24.953	533	326	15	29	523	29	525	4.19e-26	112
MS.gene30927.t1	AT4G14390	24.213	508	340	13	10	489	152	642	9.75e-26	112
MS.gene30927.t1	AT4G14390	24.213	508	340	13	10	489	133	623	9.87e-26	112
MS.gene30927.t1	AT4G03490	25.000	480	302	14	28	497	135	566	1.09e-25	111
MS.gene30927.t1	AT4G03490	25.000	480	302	14	28	497	145	576	1.17e-25	111
MS.gene30927.t1	AT4G03490	24.583	480	304	13	28	497	145	576	1.55e-25	111
MS.gene30927.t1	AT4G03480	26.494	385	222	8	63	426	309	653	3.24e-25	110
MS.gene30927.t1	AT4G03480	26.494	385	222	8	63	426	312	656	3.51e-25	110
MS.gene30927.t1	AT4G14390	24.165	509	340	14	10	489	152	643	4.83e-25	110
MS.gene30927.t1	AT4G05040	25.661	378	222	7	22	383	192	526	2.76e-23	104
MS.gene30927.t1	AT4G05040	25.661	378	222	7	22	383	192	526	2.76e-23	104
MS.gene30927.t1	AT4G05040	25.661	378	222	7	22	383	192	526	2.76e-23	104
MS.gene30927.t1	AT4G05040	25.661	378	222	7	22	383	192	526	2.76e-23	104
MS.gene30927.t1	AT4G05040	25.661	378	222	7	22	383	192	526	2.76e-23	104
MS.gene30927.t1	AT4G05040	25.661	378	222	7	22	383	192	526	2.76e-23	104
MS.gene30927.t1	AT4G05040	25.661	378	222	7	22	383	192	526	2.76e-23	104
MS.gene30927.t1	AT4G14390	24.561	399	263	10	10	380	133	521	1.86e-20	95.1
MS.gene30927.t1	AT5G54700	24.925	333	192	8	161	488	23	302	2.10e-20	93.2
MS.gene30927.t1	AT4G10720	25.223	337	207	11	105	424	41	349	7.89e-20	92.4
MS.gene30927.t1	AT4G14400	24.151	530	272	15	29	483	71	545	1.39e-19	92.8
MS.gene30927.t1	AT4G14400	24.151	530	272	15	29	483	71	545	1.39e-19	92.8
MS.gene30927.t1	AT4G14400	24.151	530	272	15	29	483	137	611	1.91e-19	92.4
MS.gene30927.t1	AT4G03450	26.186	527	260	20	23	476	101	571	2.33e-19	92.0
MS.gene30927.t1	AT4G03450	24.014	279	189	6	31	292	40	312	4.48e-11	65.9
MS.gene30927.t1	AT4G03440	25.964	389	222	11	63	426	262	609	3.25e-19	92.0
MS.gene30927.t1	AT4G03440	25.964	389	222	11	63	426	262	609	3.25e-19	92.0
MS.gene30927.t1	AT4G03440	25.964	389	222	11	63	426	269	616	3.39e-19	92.0
MS.gene30927.t1	AT4G03450	26.046	526	262	20	23	476	101	571	3.45e-19	91.7
MS.gene30927.t1	AT4G03450	24.164	269	181	6	31	282	40	302	4.79e-11	65.9
MS.gene30927.t1	AT3G04140	28.000	300	172	9	31	291	106	400	6.30e-19	90.9
MS.gene30927.t1	AT3G04140	30.049	203	121	4	96	277	97	299	3.06e-14	76.3
MS.gene30927.t1	AT4G10720	26.962	293	177	9	105	381	41	312	7.50e-19	89.7
MS.gene30927.t1	AT5G51160	23.925	372	222	9	131	458	43	397	1.60e-18	88.6
MS.gene30927.t1	AT4G03490	22.628	548	300	16	28	497	17	518	5.03e-18	88.2
MS.gene30927.t1	AT4G03490	22.628	548	300	16	28	497	27	528	5.19e-18	88.2
MS.gene30927.t1	AT5G54700	23.678	397	208	10	97	488	33	339	3.02e-17	85.1
MS.gene30927.t1	AT5G15500	23.529	340	220	8	105	426	42	359	4.82e-15	78.2
MS.gene30927.t1	AT3G01750	27.046	281	163	9	31	276	109	382	2.07e-14	76.6
MS.gene30927.t1	AT3G01750	27.046	281	163	9	31	276	109	382	2.07e-14	76.6
MS.gene30927.t1	AT2G28840	32.544	169	95	6	105	257	50	215	3.82e-13	72.0
MS.gene30927.t1	AT2G28840	28.788	198	125	4	112	294	22	218	6.29e-11	65.1
MS.gene30927.t1	AT1G14500	25.106	235	156	5	106	323	42	273	3.85e-13	72.0
MS.gene30927.t1	AT1G14500	29.870	154	104	3	108	258	8	160	1.00e-12	70.9
MS.gene30927.t1	AT2G03430	33.514	185	114	6	60	241	8	186	7.10e-13	68.9
MS.gene30927.t1	AT2G03430	29.524	210	136	7	108	312	19	221	1.34e-12	68.2
MS.gene30927.t1	AT5G54610	25.962	208	137	4	107	297	7	214	1.09e-12	70.5
MS.gene30927.t1	AT5G54610	23.469	294	187	9	105	385	41	309	6.57e-12	68.2
MS.gene30927.t1	AT4G19150	35.000	120	76	2	105	224	20	137	1.37e-12	68.2
MS.gene30927.t1	AT3G18670	21.722	511	313	20	28	483	85	563	1.89e-12	70.5
MS.gene30927.t1	AT2G24600	22.500	400	231	12	28	386	72	433	3.17e-12	69.7
MS.gene30927.t1	AT2G24600	22.500	400	231	12	28	386	72	433	3.17e-12	69.7
MS.gene30927.t1	AT5G54620	23.599	339	217	9	105	431	41	349	3.44e-12	68.9
MS.gene30927.t1	AT5G54620	26.667	195	129	3	112	292	12	206	1.64e-11	67.0
MS.gene30927.t1	AT2G26650	27.935	247	128	8	100	337	550	755	8.02e-11	65.5
MS.gene30927.t1	AT3G04710	30.435	230	141	8	31	257	283	496	8.36e-11	65.1
MS.gene30927.t1	AT5G07840	38.235	102	59	2	126	223	22	123	8.70e-11	61.6

Find 112 sgRNAs with CRISPR-Local

Find 157 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
CTTTGGGAACTATGTGTTAT+TGG	0.157747	2.2:+5774954	MS.gene30927:CDS
GGATGAATTGCTTGAATTAT+TGG	0.200470	2.2:+5772733	MS.gene30927:CDS
CGAAACAATTGACAGGAAAA+AGG	0.253323	2.2:+5772633	MS.gene30927:CDS
TGCAACAAGGACTGATAAAA+AGG	0.268824	2.2:+5773812	MS.gene30927:CDS
AACTATAATGGCTACAACTT+TGG	0.277317	2.2:+5774937	MS.gene30927:CDS
AAAATTATAAATGGTGCTTC+TGG	0.278164	2.2:-5774689	None:intergenic
TACCCGAGTTCTGGCTTTCC+TGG	0.287238	2.2:-5773961	None:intergenic
GTAGACACTGCTGAGAAAAT+AGG	0.288492	2.2:+5774305	MS.gene30927:CDS
CAGATTGTAAATATGCTTCT+AGG	0.300619	2.2:+5774233	MS.gene30927:intron
ATCCCTTCTGGAATATTGTT+TGG	0.316664	2.2:-5774641	None:intergenic
CTTTATGTTGCTGCTGAGTA+TGG	0.316873	2.2:+5772785	MS.gene30927:CDS
TATTGCACTCTTCATTTCTC+TGG	0.325254	2.2:+5774727	MS.gene30927:CDS
AATACAAAGAAAATTATAAA+TGG	0.331303	2.2:-5774698	None:intergenic
TATGTAATTTACCCGAGTTC+TGG	0.335480	2.2:-5773970	None:intergenic
TGCAAAGCTGTTGTGTTTGA+TGG	0.339635	2.2:-5773624	None:intergenic
TTTGGGAACTATGTGTTATT+GGG	0.342224	2.2:+5774955	MS.gene30927:CDS
TGAGATAGTGAAATTCCTAT+TGG	0.358809	2.2:+5773671	MS.gene30927:CDS
GCATTGTCATTTGTTGTTGT+TGG	0.360348	2.2:+5774869	MS.gene30927:CDS
CTTCATTCTGCTGCAAGAAA+TGG	0.361031	2.2:+5773744	MS.gene30927:CDS
TCAGTCCTTGTTGCAACTCC+TGG	0.362368	2.2:-5773804	None:intergenic
GAACACCATGCTCTAATAGA+AGG	0.370163	2.2:-5774346	None:intergenic
TCCACTGTCATTGACAATTC+AGG	0.372401	2.2:-5773600	None:intergenic
CATCAAACTTGAGGAACATT+AGG	0.373191	2.2:+5774999	MS.gene30927:CDS
AAAGAGCTGTTTCTCCATCT+TGG	0.374656	2.2:-5772766	None:intergenic
ACTATAATGGCTACAACTTT+GGG	0.376333	2.2:+5774938	MS.gene30927:CDS
TCAACCATGTTTATTGTTGA+AGG	0.380744	2.2:-5773906	None:intergenic
TCACCAAGTATCATCCCTTC+TGG	0.381269	2.2:-5774653	None:intergenic
CATTATAGTTGCTCCTATAA+TGG	0.392151	2.2:-5774924	None:intergenic
TGCTGCAAGAAATGGACATT+TGG	0.400973	2.2:+5773752	MS.gene30927:CDS
TCTGGCGTGTGTGTTCTAAT+TGG	0.405927	2.2:-5774463	None:intergenic
ATGGTTATATTGATGTCGTT+AGG	0.409480	2.2:+5772804	MS.gene30927:CDS
AGACATATTGAAGATCCTTA+TGG	0.411353	2.2:+5773569	MS.gene30927:intron
TTCCTAGGTGTGTGCACCAT+TGG	0.416376	2.2:-5772602	None:intergenic
CATTATAGGAGCAACTATAA+TGG	0.420585	2.2:+5774925	MS.gene30927:CDS
TATTATGATCTTGCTGGTGC+TGG	0.421698	2.2:+5772839	MS.gene30927:CDS
ATTGGAAGCAGGAAGTAGTT+TGG	0.421802	2.2:+5773689	MS.gene30927:CDS
ATTCAATATTATGATCTTGC+TGG	0.424869	2.2:+5772833	MS.gene30927:CDS
CTTCATTTCTCTGGCTGTTG+TGG	0.427415	2.2:+5774736	MS.gene30927:CDS
TAGACACTGCTGAGAAAATA+GGG	0.434367	2.2:+5774306	MS.gene30927:CDS
CTGCCATGTGAAGTGCTGTC+TGG	0.436714	2.2:-5773836	None:intergenic
GCTGCTAAACAAGGGGATAT+AGG	0.436722	2.2:+5772899	MS.gene30927:CDS
CACCATTGGTGTAGCCATGC+TGG	0.437327	2.2:-5772588	None:intergenic
TTGCATTCTGCAGCAAGAGC+AGG	0.447548	2.2:+5772668	MS.gene30927:CDS
TGGTTATATTGATGTCGTTA+GGG	0.447549	2.2:+5772805	MS.gene30927:CDS
GCACTTCTTGAGAAAGAGCC+AGG	0.460376	2.2:+5773786	MS.gene30927:CDS
GTAAGTGCTGTTAACAGATC+AGG	0.461810	2.2:+5774272	MS.gene30927:CDS
TGCTAAAAGTAACGGGAAAA+CGG	0.468457	2.2:+5773719	MS.gene30927:CDS
GAACATTAGGAAATCTTCAA+TGG	0.470623	2.2:+5775012	MS.gene30927:CDS
AAATATGCTTCTAGGACAAA+AGG	0.471296	2.2:+5774241	MS.gene30927:CDS
AGCACTTCACATGGCAGTAA+AGG	0.471764	2.2:+5773842	MS.gene30927:CDS
CAGACACGAAAACGTGTTCA+AGG	0.474794	2.2:+5774482	MS.gene30927:CDS
GGGACAAAATCTTGAGGTAG+TGG	0.477594	2.2:+5773863	MS.gene30927:CDS
CAACCATTGCTAAAAGTAAC+GGG	0.485668	2.2:+5773712	MS.gene30927:CDS
GCACTTCACATGGCAGTAAA+GGG	0.488917	2.2:+5773843	MS.gene30927:CDS
AACTACTTCCTGCTTCCAAT+AGG	0.491463	2.2:-5773686	None:intergenic
AATTAATAAGCTAATGTGGT+TGG	0.495846	2.2:+5774817	MS.gene30927:CDS
GGCTTGTGTGCTTATTTCTG+TGG	0.502072	2.2:+5774838	MS.gene30927:CDS
GTGAAATTCCTATTGGAAGC+AGG	0.510342	2.2:+5773678	MS.gene30927:CDS
ATTCCAGAAGGGATGATACT+TGG	0.510792	2.2:+5774650	MS.gene30927:CDS
CAACCAGGAAAGCCAGAACT+CGG	0.516470	2.2:+5773958	MS.gene30927:CDS
ATAGAAGGGCTTTAACATCC+TGG	0.524388	2.2:-5774331	None:intergenic
TTTCATATTGCTGCTAAACA+AGG	0.525091	2.2:+5772890	MS.gene30927:CDS
GCAACCATTGCTAAAAGTAA+CGG	0.526222	2.2:+5773711	MS.gene30927:CDS
ATAAACATGGTTGATACTAA+AGG	0.532093	2.2:+5773915	MS.gene30927:CDS
AAAATGACGAAACAATTGAC+AGG	0.537514	2.2:+5772626	MS.gene30927:CDS
ATTCAGGATGAACCTCCATA+AGG	0.538961	2.2:-5773584	None:intergenic
GGTCATTAAGCATCGAATTG+AGG	0.539272	2.2:+5774976	MS.gene30927:CDS
TGCAAGAAATGGACATTTGG+AGG	0.543958	2.2:+5773755	MS.gene30927:CDS
TGCATTCTGCAGCAAGAGCA+GGG	0.546267	2.2:+5772669	MS.gene30927:CDS
GCTGGAATTAAAGCAAGAAA+CGG	0.546789	2.2:+5772857	MS.gene30927:CDS
CAGGGAATTTAGATGTTGTG+AGG	0.549163	2.2:+5772687	MS.gene30927:CDS
GTCATTTGTTGTTGTTGGAA+AGG	0.554079	2.2:+5774874	MS.gene30927:CDS
GTGCTTGTTGATCCTTGTGG+TGG	0.555571	2.2:-5774389	None:intergenic
AAGCAAAGCGAAGAAACAAA+TGG	0.557199	2.2:+5774787	MS.gene30927:CDS
TGTTGTGGTTGTTCAAACTT+CGG	0.558193	2.2:+5774751	MS.gene30927:CDS
GATCCAAACAATATTCCAGA+AGG	0.558398	2.2:+5774638	MS.gene30927:CDS
AACACCATGCTCTAATAGAA+GGG	0.558802	2.2:-5774345	None:intergenic
AGGGAATTTAGATGTTGTGA+GGG	0.562368	2.2:+5772688	MS.gene30927:CDS
TGCTGAGAAAATAGGGAACC+AGG	0.569656	2.2:+5774313	MS.gene30927:CDS
TTCATATTGCTGCTAAACAA+GGG	0.571386	2.2:+5772891	MS.gene30927:CDS
TTGGCTAGGCAGAACCAAGA+TGG	0.572068	2.2:+5772752	MS.gene30927:CDS
GGATCATCAACAAATTGACC+AGG	0.588310	2.2:-5774620	None:intergenic
TATTCTTAATGATGCTCAAG+AGG	0.592437	2.2:+5772712	MS.gene30927:CDS
AATTGCTTGAATTATTGGCT+AGG	0.595361	2.2:+5772738	MS.gene30927:CDS
TGCTATTAGTACTGCCACAA+CGG	0.600090	2.2:-5774567	None:intergenic
AACCAGGAAAGCCAGAACTC+GGG	0.603570	2.2:+5773959	MS.gene30927:CDS
CTATAATTAATAAGCTAATG+TGG	0.603869	2.2:+5774813	MS.gene30927:CDS
AAGAGCCAGGAGTTGCAACA+AGG	0.608094	2.2:+5773799	MS.gene30927:CDS
ATCCAGCATGGCTACACCAA+TGG	0.608416	2.2:+5772586	MS.gene30927:CDS
AAAGCCCTTCTATTAGAGCA+TGG	0.609187	2.2:+5774341	MS.gene30927:CDS
TCATATTGCTGCTAAACAAG+GGG	0.609590	2.2:+5772892	MS.gene30927:CDS
TCCTGAATTGTCAATGACAG+TGG	0.613702	2.2:+5773599	MS.gene30927:CDS
CATATTGAAGATCCTTATGG+AGG	0.614144	2.2:+5773572	MS.gene30927:CDS
ATCAACAAGATGAATACAGA+AGG	0.614257	2.2:+5774518	MS.gene30927:CDS
GGGCCAGACAGCACTTCACA+TGG	0.614969	2.2:+5773833	MS.gene30927:CDS
GGTTATATTGATGTCGTTAG+GGG	0.617435	2.2:+5772806	MS.gene30927:CDS
CAACATTGCATATAGCAACC+AGG	0.627104	2.2:+5773943	MS.gene30927:CDS
CACCAATGGTGCACACACCT+AGG	0.627667	2.2:+5772600	MS.gene30927:CDS
TTGCATACAGCTGCAACACA+AGG	0.627719	2.2:+5773642	MS.gene30927:CDS
GCTCTTGCTGCAGAATGCAA+AGG	0.627727	2.2:-5772665	None:intergenic
GAATTGAGGCATCAAACTTG+AGG	0.637621	2.2:+5774990	MS.gene30927:CDS
CAAGATGGGTGAATCCAGCA+TGG	0.639004	2.2:+5772574	None:intergenic
ATCCAAACAATATTCCAGAA+GGG	0.645443	2.2:+5774639	MS.gene30927:CDS
AGATCCTTCAACAATAAACA+TGG	0.658313	2.2:+5773902	MS.gene30927:CDS
TGTGGCAGTACTAATAGCAA+CGG	0.666243	2.2:+5774571	MS.gene30927:CDS
AATAAACTCGACAACCGTTG+TGG	0.669177	2.2:+5774553	MS.gene30927:CDS
AGTAAAGGGACAAAATCTTG+AGG	0.673310	2.2:+5773857	MS.gene30927:CDS
GCTGTGCTTGTTGATCCTTG+TGG	0.678811	2.2:-5774392	None:intergenic
GATCATCAACAAATTGACCA+GGG	0.681393	2.2:-5774619	None:intergenic
CAAAGCGAAGAAACAAATGG+CGG	0.712434	2.2:+5774790	MS.gene30927:CDS
AAGTCTATAAAACCACCACA+AGG	0.715719	2.2:+5774377	MS.gene30927:CDS
TGTAAGCGACATAAAGCACG+AGG	0.760936	2.2:+5774433	MS.gene30927:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	AATACAAAGAAAATTATAAA+TGG	-	chr2.2:5774701-5774720	None:intergenic	10.0%
!!!	AATTATTTGAATTTGTTTTC+TGG	+	chr2.2:5774047-5774066	MS.gene30927:intron	15.0%
!!!	ATTGATATTCTTTAAGTTAA+GGG	+	chr2.2:5773421-5773440	MS.gene30927:intron	15.0%
!!!	TAAACAAGTATTTTGATAAA+TGG	+	chr2.2:5773389-5773408	MS.gene30927:intron	15.0%
!!	CTATAATTAATAAGCTAATG+TGG	+	chr2.2:5774813-5774832	MS.gene30927:CDS	20.0%
!!!	ATTGCTATTTAGTAAATTTG+AGG	+	chr2.2:5773448-5773467	MS.gene30927:intron	20.0%
!!!	ATTTAACACCTTCATTTTAA+AGG	+	chr2.2:5773101-5773120	MS.gene30927:intron	20.0%
!!!	GATTGATATTCTTTAAGTTA+AGG	+	chr2.2:5773420-5773439	MS.gene30927:intron	20.0%
!!!	TTGCTATTTAGTAAATTTGA+GGG	+	chr2.2:5773449-5773468	MS.gene30927:intron	20.0%
!	AAAATTATAAATGGTGCTTC+TGG	-	chr2.2:5774692-5774711	None:intergenic	25.0%
!	AAATTCCATAGACATATACT+AGG	+	chr2.2:5774003-5774022	MS.gene30927:intron	25.0%
!	AATTAATAAGCTAATGTGGT+TGG	+	chr2.2:5774817-5774836	MS.gene30927:CDS	25.0%
!	ATTCAATATTATGATCTTGC+TGG	+	chr2.2:5772833-5772852	MS.gene30927:CDS	25.0%
!!	ATAAACATGGTTGATACTAA+AGG	+	chr2.2:5773915-5773934	MS.gene30927:CDS	25.0%
!!	CCCTTTAAAAATGTGTAAAT+CGG	-	chr2.2:5773515-5773534	None:intergenic	25.0%
!!	CCGATTTACACATTTTTAAA+GGG	+	chr2.2:5773512-5773531	MS.gene30927:intron	25.0%
!!	GCAATTTTTGTTACCATTAT+AGG	+	chr2.2:5774911-5774930	MS.gene30927:CDS	25.0%
!!	TTTTAGTCCTTGAAATTATG+CGG	+	chr2.2:5772989-5773008	MS.gene30927:intron	25.0%
!!!	AATGAAGGTGTTAAATTTGA+AGG	-	chr2.2:5773097-5773116	None:intergenic	25.0%
!!!	GCATATAACCTTTAAAATGA+AGG	-	chr2.2:5773112-5773131	None:intergenic	25.0%
	AAAATGACGAAACAATTGAC+AGG	+	chr2.2:5772626-5772645	MS.gene30927:CDS	30.0%
	AACTATAATGGCTACAACTT+TGG	+	chr2.2:5774937-5774956	MS.gene30927:CDS	30.0%
	ACTATAATGGCTACAACTTT+GGG	+	chr2.2:5774938-5774957	MS.gene30927:CDS	30.0%
	AGACATATTGAAGATCCTTA+TGG	+	chr2.2:5773569-5773588	MS.gene30927:intron	30.0%
	AGACGAATGTTATCAAATAG+CGG	-	chr2.2:5773305-5773324	None:intergenic	30.0%
	AGATCCTTCAACAATAAACA+TGG	+	chr2.2:5773902-5773921	MS.gene30927:CDS	30.0%
	ATAGACATATACTAGGTACT+AGG	+	chr2.2:5774010-5774029	MS.gene30927:intron	30.0%
	ATCAACAAGATGAATACAGA+AGG	+	chr2.2:5774518-5774537	MS.gene30927:CDS	30.0%
	ATCCAAACAATATTCCAGAA+GGG	+	chr2.2:5774639-5774658	MS.gene30927:CDS	30.0%
	ATGGTTATATTGATGTCGTT+AGG	+	chr2.2:5772804-5772823	MS.gene30927:CDS	30.0%
	CAGATTGTAAATATGCTTCT+AGG	+	chr2.2:5774233-5774252	MS.gene30927:intron	30.0%
	CATTATAGGAGCAACTATAA+TGG	+	chr2.2:5774925-5774944	MS.gene30927:CDS	30.0%
	CATTATAGTTGCTCCTATAA+TGG	-	chr2.2:5774927-5774946	None:intergenic	30.0%
	CTATCCAAACCAATGAAATA+CGG	-	chr2.2:5773361-5773380	None:intergenic	30.0%
	GAAACAATTGACAGGAAAAA+GGG	+	chr2.2:5772634-5772653	MS.gene30927:CDS	30.0%
	GAACATTAGGAAATCTTCAA+TGG	+	chr2.2:5775012-5775031	MS.gene30927:CDS	30.0%
	GGATGAATTGCTTGAATTAT+TGG	+	chr2.2:5772733-5772752	MS.gene30927:CDS	30.0%
	GTGTACTAATTAGTGTATCA+TGG	+	chr2.2:5773219-5773238	MS.gene30927:intron	30.0%
	TACACATTGTTGTCAAATAG+TGG	-	chr2.2:5773204-5773223	None:intergenic	30.0%
	TAGACATATACTAGGTACTA+GGG	+	chr2.2:5774011-5774030	MS.gene30927:intron	30.0%
	TAGTACCTAGTATATGTCTA+TGG	-	chr2.2:5774011-5774030	None:intergenic	30.0%
	TATTCTTAATGATGCTCAAG+AGG	+	chr2.2:5772712-5772731	MS.gene30927:CDS	30.0%
	TCAACCATGTTTATTGTTGA+AGG	-	chr2.2:5773909-5773928	None:intergenic	30.0%
	TCAATACTTCCGTATTTCAT+TGG	+	chr2.2:5773349-5773368	MS.gene30927:intron	30.0%
	TCGCATTAATTGAACAATGT+CGG	+	chr2.2:5773053-5773072	MS.gene30927:intron	30.0%
	TGAGATAGTGAAATTCCTAT+TGG	+	chr2.2:5773671-5773690	MS.gene30927:CDS	30.0%
	TGGTTATATTGATGTCGTTA+GGG	+	chr2.2:5772805-5772824	MS.gene30927:CDS	30.0%
	TGTGATTTCAAAGAGTAAGT+AGG	+	chr2.2:5772964-5772983	MS.gene30927:intron	30.0%
	TTCATATTGCTGCTAAACAA+GGG	+	chr2.2:5772891-5772910	MS.gene30927:CDS	30.0%
	TTGTTGGAAAGGAAGAAAAA+TGG	+	chr2.2:5774885-5774904	MS.gene30927:CDS	30.0%
	TTTCATATTGCTGCTAAACA+AGG	+	chr2.2:5772890-5772909	MS.gene30927:CDS	30.0%
!	AATTGCTTGAATTATTGGCT+AGG	+	chr2.2:5772738-5772757	MS.gene30927:CDS	30.0%
!	TTTAGTGTTGCTAATTGCAA+TGG	+	chr2.2:5773279-5773298	MS.gene30927:intron	30.0%
!	TTTGGGAACTATGTGTTATT+GGG	+	chr2.2:5774955-5774974	MS.gene30927:CDS	30.0%
!!	AAATATGCTTCTAGGACAAA+AGG	+	chr2.2:5774241-5774260	MS.gene30927:CDS	30.0%
!!	ACTTACTCTTTGAAATCACA+TGG	-	chr2.2:5772964-5772983	None:intergenic	30.0%
!!	GCCGATTTACACATTTTTAA+AGG	+	chr2.2:5773511-5773530	MS.gene30927:intron	30.0%
!!	GCTGCTATTTCATAAATTTG+AGG	-	chr2.2:5773029-5773048	None:intergenic	30.0%
!!!	TGTTTCGTCATTTTTTTCCT+AGG	-	chr2.2:5772620-5772639	None:intergenic	30.0%
	AACACCATGCTCTAATAGAA+GGG	-	chr2.2:5774348-5774367	None:intergenic	35.0%
	AAGCAAAGCGAAGAAACAAA+TGG	+	chr2.2:5774787-5774806	MS.gene30927:CDS	35.0%
	AAGTCTATAAAACCACCACA+AGG	+	chr2.2:5774377-5774396	MS.gene30927:CDS	35.0%
	AAGTTGCAGAAAGTAGCTTA+TGG	-	chr2.2:5774128-5774147	None:intergenic	35.0%
	AGAACACAAAAACTAGCAGT+TGG	+	chr2.2:5773158-5773177	MS.gene30927:intron	35.0%
	AGGGAATTTAGATGTTGTGA+GGG	+	chr2.2:5772688-5772707	MS.gene30927:CDS	35.0%
	AGTAAAGGGACAAAATCTTG+AGG	+	chr2.2:5773857-5773876	MS.gene30927:CDS	35.0%
	CAACCATTGCTAAAAGTAAC+GGG	+	chr2.2:5773712-5773731	MS.gene30927:CDS	35.0%
	CATATTGAAGATCCTTATGG+AGG	+	chr2.2:5773572-5773591	MS.gene30927:CDS	35.0%
	CATCAAACTTGAGGAACATT+AGG	+	chr2.2:5774999-5775018	MS.gene30927:CDS	35.0%
	CGAAACAATTGACAGGAAAA+AGG	+	chr2.2:5772633-5772652	MS.gene30927:CDS	35.0%
	GACTAATCCGCATAATTTCA+AGG	-	chr2.2:5772999-5773018	None:intergenic	35.0%
	GATCATCAACAAATTGACCA+GGG	-	chr2.2:5774622-5774641	None:intergenic	35.0%
	GATCCAAACAATATTCCAGA+AGG	+	chr2.2:5774638-5774657	MS.gene30927:CDS	35.0%
	GCAACAAGGACTGATAAAAA+GGG	+	chr2.2:5773813-5773832	MS.gene30927:CDS	35.0%
	GCAACCATTGCTAAAAGTAA+CGG	+	chr2.2:5773711-5773730	MS.gene30927:CDS	35.0%
	GCTGGAATTAAAGCAAGAAA+CGG	+	chr2.2:5772857-5772876	MS.gene30927:CDS	35.0%
	GGTTATATTGATGTCGTTAG+GGG	+	chr2.2:5772806-5772825	MS.gene30927:CDS	35.0%
	TAGACACTGCTGAGAAAATA+GGG	+	chr2.2:5774306-5774325	MS.gene30927:CDS	35.0%
	TATGTAATTTACCCGAGTTC+TGG	-	chr2.2:5773973-5773992	None:intergenic	35.0%
	TATTGCACTCTTCATTTCTC+TGG	+	chr2.2:5774727-5774746	MS.gene30927:CDS	35.0%
	TCATATTGCTGCTAAACAAG+GGG	+	chr2.2:5772892-5772911	MS.gene30927:CDS	35.0%
	TCTTCAATATGTCTGCAACA+AGG	-	chr2.2:5773563-5773582	None:intergenic	35.0%
	TGCAACAAGGACTGATAAAA+AGG	+	chr2.2:5773812-5773831	MS.gene30927:CDS	35.0%
	TGGAAAGGAAGAAAAATGGT+TGG	+	chr2.2:5774889-5774908	MS.gene30927:CDS	35.0%
	TGTTGTGGTTGTTCAAACTT+CGG	+	chr2.2:5774751-5774770	MS.gene30927:CDS	35.0%
	TTGAAATCCGTCATTGACAA+TGG	+	chr2.2:5774152-5774171	MS.gene30927:intron	35.0%
!	ACTTCCGTATTTCATTGGTT+TGG	+	chr2.2:5773354-5773373	MS.gene30927:intron	35.0%
!	CTTTGGGAACTATGTGTTAT+TGG	+	chr2.2:5774954-5774973	MS.gene30927:CDS	35.0%
!	TCCTTTTCCATTGTCAATGA+CGG	-	chr2.2:5774162-5774181	None:intergenic	35.0%
!	TGCTAAAAGTAACGGGAAAA+CGG	+	chr2.2:5773719-5773738	MS.gene30927:CDS	35.0%
!!	ATCCCTTCTGGAATATTGTT+TGG	-	chr2.2:5774644-5774663	None:intergenic	35.0%
!!	GCATTGTCATTTGTTGTTGT+TGG	+	chr2.2:5774869-5774888	MS.gene30927:CDS	35.0%
!!	GTCATTTGTTGTTGTTGGAA+AGG	+	chr2.2:5774874-5774893	MS.gene30927:CDS	35.0%
!!	TAAGCACTTCTGAAACTGTT+TGG	+	chr2.2:5774072-5774091	MS.gene30927:intron	35.0%
!!	TATTGACATCTGTTTCTGAG+AGG	+	chr2.2:5774186-5774205	MS.gene30927:intron	35.0%
!!!	TTTCCCGTTACTTTTAGCAA+TGG	-	chr2.2:5773718-5773737	None:intergenic	35.0%
	AAAGCCCTTCTATTAGAGCA+TGG	+	chr2.2:5774341-5774360	MS.gene30927:CDS	40.0%
	AACTACTTCCTGCTTCCAAT+AGG	-	chr2.2:5773689-5773708	None:intergenic	40.0%
	AATAAACTCGACAACCGTTG+TGG	+	chr2.2:5774553-5774572	MS.gene30927:CDS	40.0%
	ATTCAGGATGAACCTCCATA+AGG	-	chr2.2:5773587-5773606	None:intergenic	40.0%
	ATTCCAGAAGGGATGATACT+TGG	+	chr2.2:5774650-5774669	MS.gene30927:CDS	40.0%
	ATTGGAAGCAGGAAGTAGTT+TGG	+	chr2.2:5773689-5773708	MS.gene30927:CDS	40.0%
	CAAAGCGAAGAAACAAATGG+CGG	+	chr2.2:5774790-5774809	MS.gene30927:CDS	40.0%
	CAACATTGCATATAGCAACC+AGG	+	chr2.2:5773943-5773962	MS.gene30927:CDS	40.0%
	CAGGGAATTTAGATGTTGTG+AGG	+	chr2.2:5772687-5772706	MS.gene30927:CDS	40.0%
	CTTCATTCTGCTGCAAGAAA+TGG	+	chr2.2:5773744-5773763	MS.gene30927:CDS	40.0%
	CTTTATGTTGCTGCTGAGTA+TGG	+	chr2.2:5772785-5772804	MS.gene30927:CDS	40.0%
	GAACACCATGCTCTAATAGA+AGG	-	chr2.2:5774349-5774368	None:intergenic	40.0%
	GGATCATCAACAAATTGACC+AGG	-	chr2.2:5774623-5774642	None:intergenic	40.0%
	GTAGACACTGCTGAGAAAAT+AGG	+	chr2.2:5774305-5774324	MS.gene30927:CDS	40.0%
	GTGAAATTCCTATTGGAAGC+AGG	+	chr2.2:5773678-5773697	MS.gene30927:CDS	40.0%
	TATTATGATCTTGCTGGTGC+TGG	+	chr2.2:5772839-5772858	MS.gene30927:CDS	40.0%
	TCCACTGTCATTGACAATTC+AGG	-	chr2.2:5773603-5773622	None:intergenic	40.0%
	TCCGTCATTGACAATGGAAA+AGG	+	chr2.2:5774158-5774177	MS.gene30927:intron	40.0%
	TCCTGAATTGTCAATGACAG+TGG	+	chr2.2:5773599-5773618	MS.gene30927:CDS	40.0%
	TGCAAAGCTGTTGTGTTTGA+TGG	-	chr2.2:5773627-5773646	None:intergenic	40.0%
	TGCAAGAAATGGACATTTGG+AGG	+	chr2.2:5773755-5773774	MS.gene30927:CDS	40.0%
	TGCTATTAGTACTGCCACAA+CGG	-	chr2.2:5774570-5774589	None:intergenic	40.0%
	TGCTGCAAGAAATGGACATT+TGG	+	chr2.2:5773752-5773771	MS.gene30927:CDS	40.0%
	TGTGGCAGTACTAATAGCAA+CGG	+	chr2.2:5774571-5774590	MS.gene30927:CDS	40.0%
!	ATAGAAGGGCTTTAACATCC+TGG	-	chr2.2:5774334-5774353	None:intergenic	40.0%
!	GAATTGAGGCATCAAACTTG+AGG	+	chr2.2:5774990-5775009	MS.gene30927:CDS	40.0%
!	GTAAGTGCTGTTAACAGATC+AGG	+	chr2.2:5774272-5774291	MS.gene30927:CDS	40.0%
!!	AAAGAGCTGTTTCTCCATCT+TGG	-	chr2.2:5772769-5772788	None:intergenic	40.0%
!!	GGTCATTAAGCATCGAATTG+AGG	+	chr2.2:5774976-5774995	MS.gene30927:CDS	40.0%
	AGCACTTCACATGGCAGTAA+AGG	+	chr2.2:5773842-5773861	MS.gene30927:CDS	45.0%
	CAGACACGAAAACGTGTTCA+AGG	+	chr2.2:5774482-5774501	MS.gene30927:CDS	45.0%
	CTTCATTTCTCTGGCTGTTG+TGG	+	chr2.2:5774736-5774755	MS.gene30927:CDS	45.0%
	GCACTTCACATGGCAGTAAA+GGG	+	chr2.2:5773843-5773862	MS.gene30927:CDS	45.0%
	GCTGCTAAACAAGGGGATAT+AGG	+	chr2.2:5772899-5772918	MS.gene30927:CDS	45.0%
	GGGACAAAATCTTGAGGTAG+TGG	+	chr2.2:5773863-5773882	MS.gene30927:CDS	45.0%
	TCACCAAGTATCATCCCTTC+TGG	-	chr2.2:5774656-5774675	None:intergenic	45.0%
	TCTGGCGTGTGTGTTCTAAT+TGG	-	chr2.2:5774466-5774485	None:intergenic	45.0%
	TGCTGAGAAAATAGGGAACC+AGG	+	chr2.2:5774313-5774332	MS.gene30927:CDS	45.0%
	TGTAAGCGACATAAAGCACG+AGG	+	chr2.2:5774433-5774452	MS.gene30927:CDS	45.0%
	TTGCATACAGCTGCAACACA+AGG	+	chr2.2:5773642-5773661	MS.gene30927:CDS	45.0%
!	CTTGAACACGTTTTCGTGTC+TGG	-	chr2.2:5774484-5774503	None:intergenic	45.0%
!!	GGCTTGTGTGCTTATTTCTG+TGG	+	chr2.2:5774838-5774857	MS.gene30927:CDS	45.0%
	AACCAGGAAAGCCAGAACTC+GGG	+	chr2.2:5773959-5773978	MS.gene30927:CDS	50.0%
	AAGAGCCAGGAGTTGCAACA+AGG	+	chr2.2:5773799-5773818	MS.gene30927:CDS	50.0%
	ATCCAGCATGGCTACACCAA+TGG	+	chr2.2:5772586-5772605	MS.gene30927:CDS	50.0%
	CAACCAGGAAAGCCAGAACT+CGG	+	chr2.2:5773958-5773977	MS.gene30927:CDS	50.0%
	GCACTTCTTGAGAAAGAGCC+AGG	+	chr2.2:5773786-5773805	MS.gene30927:CDS	50.0%
	GCTCTTGCTGCAGAATGCAA+AGG	-	chr2.2:5772668-5772687	None:intergenic	50.0%
	TCAGTCCTTGTTGCAACTCC+TGG	-	chr2.2:5773807-5773826	None:intergenic	50.0%
	TGCATTCTGCAGCAAGAGCA+GGG	+	chr2.2:5772669-5772688	MS.gene30927:CDS	50.0%
	TTCCTAGGTGTGTGCACCAT+TGG	-	chr2.2:5772605-5772624	None:intergenic	50.0%
	TTGCATTCTGCAGCAAGAGC+AGG	+	chr2.2:5772668-5772687	MS.gene30927:CDS	50.0%
	TTGGCTAGGCAGAACCAAGA+TGG	+	chr2.2:5772752-5772771	MS.gene30927:CDS	50.0%
!	GCTGTGCTTGTTGATCCTTG+TGG	-	chr2.2:5774395-5774414	None:intergenic	50.0%
!	GTGCTTGTTGATCCTTGTGG+TGG	-	chr2.2:5774392-5774411	None:intergenic	50.0%
	CACCAATGGTGCACACACCT+AGG	+	chr2.2:5772600-5772619	MS.gene30927:CDS	55.0%
	CACCATTGGTGTAGCCATGC+TGG	-	chr2.2:5772591-5772610	None:intergenic	55.0%
!	GCTGCGATTTTCACTGTCCC+TGG	+	chr2.2:5774602-5774621	MS.gene30927:CDS	55.0%
!!	CTGCCATGTGAAGTGCTGTC+TGG	-	chr2.2:5773839-5773858	None:intergenic	55.0%
!!	TACCCGAGTTCTGGCTTTCC+TGG	-	chr2.2:5773964-5773983	None:intergenic	55.0%
	GGGCCAGACAGCACTTCACA+TGG	+	chr2.2:5773833-5773852	MS.gene30927:CDS	60.0%

Chromosome	Type	Strat	End	Strand	Name
chr2.2	gene	5772578	5775120	5772578	ID=MS.gene30927
chr2.2	mRNA	5772578	5775120	5772578	ID=MS.gene30927.t1;Parent=MS.gene30927
chr2.2	exon	5772578	5772920	5772578	ID=MS.gene30927.t1.exon1;Parent=MS.gene30927.t1
chr2.2	CDS	5772578	5772920	5772578	ID=cds.MS.gene30927.t1;Parent=MS.gene30927.t1
chr2.2	exon	5773571	5773980	5773571	ID=MS.gene30927.t1.exon2;Parent=MS.gene30927.t1
chr2.2	CDS	5773571	5773980	5773571	ID=cds.MS.gene30927.t1;Parent=MS.gene30927.t1
chr2.2	exon	5774236	5775120	5774236	ID=MS.gene30927.t1.exon3;Parent=MS.gene30927.t1
chr2.2	CDS	5774236	5775120	5774236	ID=cds.MS.gene30927.t1;Parent=MS.gene30927.t1

Gene Sequence

>MS.gene30927

ATGGGTGAATCCAGCATGGCTACACCAATGGTGCACACACCTAGGAAAAAAATGACGAAACAATTGACAGGAAAAAGGGATGATACACCTTTGCATTCTGCAGCAAGAGCAGGGAATTTAGATGTTGTGAGGGATATTCTTAATGATGCTCAAGAGGATGAATTGCTTGAATTATTGGCTAGGCAGAACCAAGATGGAGAAACAGCTCTTTATGTTGCTGCTGAGTATGGTTATATTGATGTCGTTAGGGGAATGATTCAATATTATGATCTTGCTGGTGCTGGAATTAAAGCAAGAAACGGTTTTGATGCATTTCATATTGCTGCTAAACAAGGGGATATAGGTATTTTCATCATCTTTGTTGTTTATTTGTTTGTTTTGTTCCATGTGATTTCAAAGAGTAAGTAGGCATTTTAGTCCTTGAAATTATGCGGATTAGTCAATTTAGCCTCAAATTTATGAAATAGCAGCTTAGTCGCATTAATTGAACAATGTCGGTCAATTTAATCTTTTTTTCCTTCAAATTTAACACCTTCATTTTAAAGGTTATATGCTTGTCTTGAGCAGTGTTGTCGATCACAGAACACAAAAACTAGCAGTTGGTTCAATTTTTGCTACGCTAGCCACTATTTGACAACAATGTGTACTAATTAGTGTATCATGGAACAATAATATGTTCAAATACTGCAACGCTATAGTGTTTTAGTGTTGCTAATTGCAATGGCCGCTATTTGATAACATTCGTCTTCATGTTGCAAGAGACTACTCAAGTCAATACTTCCGTATTTCATTGGTTTGGATAGATGTTTATTAAACAAGTATTTTGATAAATGGACACAATTGATTGATATTCTTTAAGTTAAGGGTTTAATTGCTATTTAGTAAATTTGAGGGACTAAATTGAACAGCTATTACAATTTCAATGACTAAAATGCCGATTTACACATTTTTAAAGGGAATCTCATTATCTGAAATATTTTTGCCTTGTTGCAGACATATTGAAGATCCTTATGGAGGTTCATCCTGAATTGTCAATGACAGTGGATCCATCAAACACAACAGCTTTGCATACAGCTGCAACACAAGGACATATTGAGATAGTGAAATTCCTATTGGAAGCAGGAAGTAGTTTGGCAACCATTGCTAAAAGTAACGGGAAAACGGCTCTTCATTCTGCTGCAAGAAATGGACATTTGGAGGTTGTGAAAGCACTTCTTGAGAAAGAGCCAGGAGTTGCAACAAGGACTGATAAAAAGGGCCAGACAGCACTTCACATGGCAGTAAAGGGACAAAATCTTGAGGTAGTGGAAGAGTTGATAAAAGCAGATCCTTCAACAATAAACATGGTTGATACTAAAGGAAACACAACATTGCATATAGCAACCAGGAAAGCCAGAACTCGGGTAAATTACATAATGCTTGTCAAAATTCCATAGACATATACTAGGTACTAGGGTCTGTTTGTATTGAATTATTTGAATTTGTTTTCTGGCATAAGCACTTCTGAAACTGTTTGGAAGTGCTTGTGAAAACAGCTTATGACACATCCATAAGCTACTTTCTGCAACTTAATTTTGAAATCCGTCATTGACAATGGAAAAGGAAAATTATTGACATCTGTTTCTGAGAGGCTAAAATATGTAAATTTGTTTGTGCAGATTGTAAATATGCTTCTAGGACAAAAGGAAACAAATGTAAGTGCTGTTAACAGATCAGGTGAAACAGCAGTAGACACTGCTGAGAAAATAGGGAACCAGGATGTTAAAGCCCTTCTATTAGAGCATGGTGTTCAAAGTGCAAAGTCTATAAAACCACCACAAGGATCAACAAGCACAGCTCGCGAGTTAAAGCAAACTGTAAGCGACATAAAGCACGAGGTACATTACCAATTAGAACACACACGCCAGACACGAAAACGTGTTCAAGGAATCGCAAAACGTATCAACAAGATGAATACAGAAGGACTCAACAATGCAATAAACTCGACAACCGTTGTGGCAGTACTAATAGCAACGGTTGCATTTGCTGCGATTTTCACTGTCCCTGGTCAATTTGTTGATGATCCAAACAATATTCCAGAAGGGATGATACTTGGTGAAGCAAATATATCTCCAGAAGCACCATTTATAATTTTCTTTGTATTTGATTCTATTGCACTCTTCATTTCTCTGGCTGTTGTGGTTGTTCAAACTTCGGTTGTCGTTATAGAAAGCAAAGCGAAGAAACAAATGGCGGCTATAATTAATAAGCTAATGTGGTTGGCTTGTGTGCTTATTTCTGTGGCATTTTTAGCATTGTCATTTGTTGTTGTTGGAAAGGAAGAAAAATGGTTGGCAATTTTTGTTACCATTATAGGAGCAACTATAATGGCTACAACTTTGGGAACTATGTGTTATTGGGTCATTAAGCATCGAATTGAGGCATCAAACTTGAGGAACATTAGGAAATCTTCAATGGAAAGCAAATCAAAGTCTTTTTCAGTGACAGCTTTGTCAGATTCTGAGCTAATGAACAATGAATTTAAGAAAATGTATGCTATTTAG

Protein sequence

>MS.gene30927.t1

MGESSMATPMVHTPRKKMTKQLTGKRDDTPLHSAARAGNLDVVRDILNDAQEDELLELLARQNQDGETALYVAAEYGYIDVVRGMIQYYDLAGAGIKARNGFDAFHIAAKQGDIDILKILMEVHPELSMTVDPSNTTALHTAATQGHIEIVKFLLEAGSSLATIAKSNGKTALHSAARNGHLEVVKALLEKEPGVATRTDKKGQTALHMAVKGQNLEVVEELIKADPSTINMVDTKGNTTLHIATRKARTRIVNMLLGQKETNVSAVNRSGETAVDTAEKIGNQDVKALLLEHGVQSAKSIKPPQGSTSTARELKQTVSDIKHEVHYQLEHTRQTRKRVQGIAKRINKMNTEGLNNAINSTTVVAVLIATVAFAAIFTVPGQFVDDPNNIPEGMILGEANISPEAPFIIFFVFDSIALFISLAVVVVQTSVVVIESKAKKQMAAIINKLMWLACVLISVAFLALSFVVVGKEEKWLAIFVTIIGATIMATTLGTMCYWVIKHRIEASNLRNIRKSSMESKSKSFSVTALSDSELMNNEFKKMYAI