Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene34318.t1 | XP_013454491.1 | 99.3 | 151 | 1 | 0 | 1 | 151 | 83 | 233 | 1.20E-83 | 318.9 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene34318.t1 | A0A072UFR3 | 99.3 | 151 | 1 | 0 | 1 | 151 | 83 | 233 | 8.9e-84 | 318.9 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049137 | MS.gene34318 | 0.834368 | 3.12E-56 | -1.69E-46 |
| MS.gene049427 | MS.gene34318 | 0.818888 | 1.55E-52 | -1.69E-46 |
| MS.gene049805 | MS.gene34318 | 0.801601 | 8.34E-49 | -1.69E-46 |
| MS.gene050432 | MS.gene34318 | 0.804175 | 2.45E-49 | -1.69E-46 |
| MS.gene050670 | MS.gene34318 | 0.824898 | 6.29E-54 | -1.69E-46 |
| MS.gene050747 | MS.gene34318 | 0.805566 | 1.25E-49 | -1.69E-46 |
| MS.gene050812 | MS.gene34318 | 0.807903 | 4.03E-50 | -1.69E-46 |
| MS.gene05081 | MS.gene34318 | 0.805928 | 1.05E-49 | -1.69E-46 |
| MS.gene05105 | MS.gene34318 | 0.820067 | 8.34E-53 | -1.69E-46 |
| MS.gene051582 | MS.gene34318 | 0.828553 | 8.43E-55 | -1.69E-46 |
| MS.gene051666 | MS.gene34318 | 0.836505 | 9.00E-57 | -1.69E-46 |
| MS.gene051838 | MS.gene34318 | 0.829 | 6.58E-55 | -1.69E-46 |
| MS.gene052134 | MS.gene34318 | 0.807826 | 4.18E-50 | -1.69E-46 |
| MS.gene052135 | MS.gene34318 | 0.806693 | 7.27E-50 | -1.69E-46 |
| MS.gene052414 | MS.gene34318 | 0.801086 | 1.06E-48 | -1.69E-46 |
| MS.gene052481 | MS.gene34318 | 0.845518 | 3.86E-59 | -1.69E-46 |
| MS.gene052484 | MS.gene34318 | 0.823279 | 1.51E-53 | -1.69E-46 |
| MS.gene052501 | MS.gene34318 | 0.834518 | 2.86E-56 | -1.69E-46 |
| MS.gene052505 | MS.gene34318 | 0.834419 | 3.03E-56 | -1.69E-46 |
| MS.gene052982 | MS.gene34318 | 0.82356 | 1.30E-53 | -1.69E-46 |
| MS.gene053103 | MS.gene34318 | 0.82665 | 2.42E-54 | -1.69E-46 |
| MS.gene053104 | MS.gene34318 | 0.804343 | 2.26E-49 | -1.69E-46 |
| MS.gene053207 | MS.gene34318 | 0.816555 | 5.21E-52 | -1.69E-46 |
| MS.gene053209 | MS.gene34318 | 0.808663 | 2.77E-50 | -1.69E-46 |
| MS.gene053632 | MS.gene34318 | 0.809414 | 1.91E-50 | -1.69E-46 |
| MS.gene055116 | MS.gene34318 | 0.808616 | 2.84E-50 | -1.69E-46 |
| MS.gene055206 | MS.gene34318 | 0.812317 | 4.51E-51 | -1.69E-46 |
| MS.gene055289 | MS.gene34318 | 0.817002 | 4.13E-52 | -1.69E-46 |
| MS.gene055370 | MS.gene34318 | 0.813438 | 2.56E-51 | -1.69E-46 |
| MS.gene055539 | MS.gene34318 | 0.812283 | 4.58E-51 | -1.69E-46 |
| MS.gene05553 | MS.gene34318 | 0.802205 | 6.27E-49 | -1.69E-46 |
| MS.gene057124 | MS.gene34318 | 0.844938 | 5.54E-59 | -1.69E-46 |
| MS.gene057196 | MS.gene34318 | 0.816429 | 5.56E-52 | -1.69E-46 |
| MS.gene057658 | MS.gene34318 | 0.827532 | 1.49E-54 | -1.69E-46 |
| MS.gene058233 | MS.gene34318 | 0.828263 | 9.91E-55 | -1.69E-46 |
| MS.gene058365 | MS.gene34318 | 0.806779 | 6.97E-50 | -1.69E-46 |
| MS.gene058746 | MS.gene34318 | 0.809603 | 1.74E-50 | -1.69E-46 |
| MS.gene058838 | MS.gene34318 | 0.804353 | 2.25E-49 | -1.69E-46 |
| MS.gene05914 | MS.gene34318 | 0.805494 | 1.30E-49 | -1.69E-46 |
| MS.gene05934 | MS.gene34318 | 0.801919 | 7.17E-49 | -1.69E-46 |
| MS.gene059688 | MS.gene34318 | 0.823476 | 1.36E-53 | -1.69E-46 |
| MS.gene059692 | MS.gene34318 | 0.826701 | 2.35E-54 | -1.69E-46 |
| MS.gene059695 | MS.gene34318 | 0.828508 | 8.65E-55 | -1.69E-46 |
| MS.gene060403 | MS.gene34318 | 0.832393 | 9.70E-56 | -1.69E-46 |
| MS.gene060407 | MS.gene34318 | 0.816324 | 5.86E-52 | -1.69E-46 |
| MS.gene06092 | MS.gene34318 | 0.844372 | 7.88E-59 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene34318.t1 | MTR_4g005360 | 99.338 | 151 | 1 | 0 | 1 | 151 | 83 | 233 | 7.44e-111 | 314 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene34318.t1 | AT3G20270 | 57.241 | 145 | 60 | 2 | 1 | 145 | 90 | 232 | 8.53e-54 | 180 |
| MS.gene34318.t1 | AT3G20270 | 57.241 | 145 | 60 | 2 | 1 | 145 | 90 | 232 | 8.53e-54 | 180 |
Find 35 sgRNAs with CRISPR-Local
Find 59 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GCTTGCTGTTCTTGACAATT+TGG | 0.246299 | 4.3:+89114226 | MS.gene34318:CDS |
| ATCTCATCCACTTGTATATC+AGG | 0.333453 | 4.3:-89113761 | None:intergenic |
| ACTGATCTAGTGTAAGGATA+TGG | 0.366614 | 4.3:-89114510 | None:intergenic |
| ATGAACTTGTATCTCGAATC+TGG | 0.411354 | 4.3:+89114204 | MS.gene34318:CDS |
| CCAATACCATCTGCTTGGTT+AGG | 0.422828 | 4.3:+89114639 | MS.gene34318:CDS |
| TATTTGTTGACTTATATGTA+AGG | 0.446098 | 4.3:-89113726 | None:intergenic |
| CCTAACCAAGCAGATGGTAT+TGG | 0.447991 | 4.3:-89114639 | None:intergenic |
| ATACTTCACGGAACCATGAT+TGG | 0.450822 | 4.3:+89113851 | MS.gene34318:CDS |
| TACAAGTGGATGAGATTGTT+GGG | 0.457723 | 4.3:+89113768 | MS.gene34318:CDS |
| TGTTGGCATGTGCTAAAAGT+AGG | 0.469168 | 4.3:+89114610 | MS.gene34318:CDS |
| ACTTTGACTGATCTAGTGTA+AGG | 0.474599 | 4.3:-89114516 | None:intergenic |
| TCCACCATGAGGAGGCATAA+TGG | 0.492329 | 4.3:-89113828 | None:intergenic |
| TTCAGCCAATACCATCTGCT+TGG | 0.495555 | 4.3:+89114634 | MS.gene34318:CDS |
| GCAGATCAGTTTATTGCCAA+TGG | 0.496622 | 4.3:+89114161 | MS.gene34318:intron |
| AGATTCGAGATACAAGTTCA+TGG | 0.497424 | 4.3:-89114202 | None:intergenic |
| CTAGATCAGTCAAAGTTCAA+TGG | 0.503366 | 4.3:+89114523 | MS.gene34318:CDS |
| CCTCATGGTGGAATACTTCA+CGG | 0.510920 | 4.3:+89113839 | MS.gene34318:CDS |
| TACGTCTCTTGACTTGCCAT+TGG | 0.513346 | 4.3:-89114177 | None:intergenic |
| TGTAGATTATTATGATATGT+TGG | 0.518385 | 4.3:+89114593 | MS.gene34318:CDS |
| CTTAAACGCCTTGCACATGA+AGG | 0.518985 | 4.3:+89114275 | MS.gene34318:CDS |
| AAGGCGTTTAAGTAAGCAAA+AGG | 0.541095 | 4.3:-89114264 | None:intergenic |
| GGGGCATGCCATACCAATCA+TGG | 0.551161 | 4.3:-89113864 | None:intergenic |
| CTTTCCATTATGCCTCCTCA+TGG | 0.553623 | 4.3:+89113824 | MS.gene34318:CDS |
| ATACAAGTGGATGAGATTGT+TGG | 0.562800 | 4.3:+89113767 | MS.gene34318:CDS |
| CTACATGGTTGAAGCAATCA+CGG | 0.564717 | 4.3:-89114575 | None:intergenic |
| GATTCGAGATACAAGTTCAT+GGG | 0.566517 | 4.3:-89114201 | None:intergenic |
| GATCAGTCAAAGTTCAATGG+AGG | 0.590714 | 4.3:+89114526 | MS.gene34318:CDS |
| ACATATCATAATAATCTACA+TGG | 0.601342 | 4.3:-89114590 | None:intergenic |
| ATCAGTCAAAGTTCAATGGA+GGG | 0.611627 | 4.3:+89114527 | MS.gene34318:CDS |
| TAATAACCTAACCAAGCAGA+TGG | 0.613735 | 4.3:-89114645 | None:intergenic |
| TTACATCTCCTTCATGTGCA+AGG | 0.614326 | 4.3:-89114283 | None:intergenic |
| TCCATTATGCCTCCTCATGG+TGG | 0.618370 | 4.3:+89113827 | MS.gene34318:CDS |
| TTCTGATCCTGATATACAAG+TGG | 0.625145 | 4.3:+89113754 | MS.gene34318:CDS |
| TGAAGTATTCCACCATGAGG+AGG | 0.648479 | 4.3:-89113836 | None:intergenic |
| CCGTGAAGTATTCCACCATG+AGG | 0.714100 | 4.3:-89113839 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | ACATATCATAATAATCTACA+TGG | - | chr4.3:89114593-89114612 | None:intergenic | 20.0% |
| !! | TGTAGATTATTATGATATGT+TGG | + | chr4.3:89114593-89114612 | MS.gene34318:CDS | 20.0% |
| !! | TTCATTTCAATTAGAATCAT+AGG | - | chr4.3:89114034-89114053 | None:intergenic | 20.0% |
| !!! | TATTTGTTGACTTATATGTA+AGG | - | chr4.3:89113729-89113748 | None:intergenic | 20.0% |
| ! | ATTTCTTAGATTTGTGCAAA+AGG | + | chr4.3:89114378-89114397 | MS.gene34318:intron | 25.0% |
| ! | TGTAACAACAAAAGTAAAGA+AGG | - | chr4.3:89113891-89113910 | None:intergenic | 25.0% |
| !! | AATGAATTAGTTCTCTAAGT+TGG | + | chr4.3:89114048-89114067 | MS.gene34318:intron | 25.0% |
| !! | CTTATATCTTTATGTCCTTT+TGG | + | chr4.3:89113911-89113930 | MS.gene34318:intron | 25.0% |
| AAAGAGACAAAATGTTGATG+AGG | - | chr4.3:89114446-89114465 | None:intergenic | 30.0% | |
| AACAACAAAAGTAAAGAAGG+TGG | - | chr4.3:89113888-89113907 | None:intergenic | 30.0% | |
| ACAACAAAAGTAAAGAAGGT+GGG | - | chr4.3:89113887-89113906 | None:intergenic | 30.0% | |
| AGGATCAATTCAGTTTGATA+TGG | - | chr4.3:89114014-89114033 | None:intergenic | 30.0% | |
| TTGGTTTATCTTGTTCTTGT+TGG | + | chr4.3:89114067-89114086 | MS.gene34318:intron | 30.0% | |
| ! | TTTTTGCAATAACTTTCAGC+AGG | + | chr4.3:89114479-89114498 | MS.gene34318:intron | 30.0% |
| ACTCGACATAAGAGTTGTTA+CGG | - | chr4.3:89113943-89113962 | None:intergenic | 35.0% | |
| ACTGATCTAGTGTAAGGATA+TGG | - | chr4.3:89114513-89114532 | None:intergenic | 35.0% | |
| ACTTTGACTGATCTAGTGTA+AGG | - | chr4.3:89114519-89114538 | None:intergenic | 35.0% | |
| AGATTCGAGATACAAGTTCA+TGG | - | chr4.3:89114205-89114224 | None:intergenic | 35.0% | |
| ATACAAGTGGATGAGATTGT+TGG | + | chr4.3:89113767-89113786 | MS.gene34318:CDS | 35.0% | |
| ATCAGTCAAAGTTCAATGGA+GGG | + | chr4.3:89114527-89114546 | MS.gene34318:CDS | 35.0% | |
| ATCTCATCCACTTGTATATC+AGG | - | chr4.3:89113764-89113783 | None:intergenic | 35.0% | |
| ATGAACTTGTATCTCGAATC+TGG | + | chr4.3:89114204-89114223 | MS.gene34318:CDS | 35.0% | |
| CAACAAAAGTAAAGAAGGTG+GGG | - | chr4.3:89113886-89113905 | None:intergenic | 35.0% | |
| CTAGATCAGTCAAAGTTCAA+TGG | + | chr4.3:89114523-89114542 | MS.gene34318:CDS | 35.0% | |
| GATTCGAGATACAAGTTCAT+GGG | - | chr4.3:89114204-89114223 | None:intergenic | 35.0% | |
| TAATAACCTAACCAAGCAGA+TGG | - | chr4.3:89114648-89114667 | None:intergenic | 35.0% | |
| TACAAGTGGATGAGATTGTT+GGG | + | chr4.3:89113768-89113787 | MS.gene34318:CDS | 35.0% | |
| TTCTGATCCTGATATACAAG+TGG | + | chr4.3:89113754-89113773 | MS.gene34318:CDS | 35.0% | |
| TTGTAGGAGAGAGGTTAATA+TGG | + | chr4.3:89113964-89113983 | MS.gene34318:intron | 35.0% | |
| TTGTTACGGGATAAACCAAA+AGG | - | chr4.3:89113929-89113948 | None:intergenic | 35.0% | |
| ! | AAGGCGTTTAAGTAAGCAAA+AGG | - | chr4.3:89114267-89114286 | None:intergenic | 35.0% |
| !! | ACTCTTATGTCGAGTTTTGT+AGG | + | chr4.3:89113948-89113967 | MS.gene34318:intron | 35.0% |
| ATACTTCACGGAACCATGAT+TGG | + | chr4.3:89113851-89113870 | MS.gene34318:CDS | 40.0% | |
| CTACATGGTTGAAGCAATCA+CGG | - | chr4.3:89114578-89114597 | None:intergenic | 40.0% | |
| CTCGACATAAGAGTTGTTAC+GGG | - | chr4.3:89113942-89113961 | None:intergenic | 40.0% | |
| GATCAGTCAAAGTTCAATGG+AGG | + | chr4.3:89114526-89114545 | MS.gene34318:CDS | 40.0% | |
| GCAGATCAGTTTATTGCCAA+TGG | + | chr4.3:89114161-89114180 | MS.gene34318:intron | 40.0% | |
| TGTTGGCATGTGCTAAAAGT+AGG | + | chr4.3:89114610-89114629 | MS.gene34318:CDS | 40.0% | |
| TTACATCTCCTTCATGTGCA+AGG | - | chr4.3:89114286-89114305 | None:intergenic | 40.0% | |
| !! | GCTTGCTGTTCTTGACAATT+TGG | + | chr4.3:89114226-89114245 | MS.gene34318:CDS | 40.0% |
| CCAATACCATCTGCTTGGTT+AGG | + | chr4.3:89114639-89114658 | MS.gene34318:CDS | 45.0% | |
| CCTAACCAAGCAGATGGTAT+TGG | - | chr4.3:89114642-89114661 | None:intergenic | 45.0% | |
| CTTAAACGCCTTGCACATGA+AGG | + | chr4.3:89114275-89114294 | MS.gene34318:CDS | 45.0% | |
| CTTTCCATTATGCCTCCTCA+TGG | + | chr4.3:89113824-89113843 | MS.gene34318:CDS | 45.0% | |
| TCACGGAACCATGATTGGTA+TGG | + | chr4.3:89113856-89113875 | MS.gene34318:intron | 45.0% | |
| TTCAGCCAATACCATCTGCT+TGG | + | chr4.3:89114634-89114653 | MS.gene34318:CDS | 45.0% | |
| ! | CCTCATGGTGGAATACTTCA+CGG | + | chr4.3:89113839-89113858 | MS.gene34318:CDS | 45.0% |
| ! | TGAAGTATTCCACCATGAGG+AGG | - | chr4.3:89113839-89113858 | None:intergenic | 45.0% |
| !! | TACGTCTCTTGACTTGCCAT+TGG | - | chr4.3:89114180-89114199 | None:intergenic | 45.0% |
| !! | TGTCGAGTTTTGTAGGAGAG+AGG | + | chr4.3:89113955-89113974 | MS.gene34318:intron | 45.0% |
| !! | AAATAATGATAAAAATATAT+AGG | - | chr4.3:89114362-89114381 | None:intergenic | 5.0% |
| CGCCCATAATCTAGCAAAGG+CGG | + | chr4.3:89113988-89114007 | MS.gene34318:intron | 50.0% | |
| GGCCGCCTTTGCTAGATTAT+GGG | - | chr4.3:89113993-89114012 | None:intergenic | 50.0% | |
| GGTCGCCCATAATCTAGCAA+AGG | + | chr4.3:89113985-89114004 | MS.gene34318:intron | 50.0% | |
| TCCATTATGCCTCCTCATGG+TGG | + | chr4.3:89113827-89113846 | MS.gene34318:CDS | 50.0% | |
| TGGCCGCCTTTGCTAGATTA+TGG | - | chr4.3:89113994-89114013 | None:intergenic | 50.0% | |
| ! | CCGTGAAGTATTCCACCATG+AGG | - | chr4.3:89113842-89113861 | None:intergenic | 50.0% |
| ! | TCCACCATGAGGAGGCATAA+TGG | - | chr4.3:89113831-89113850 | None:intergenic | 50.0% |
| GGGGCATGCCATACCAATCA+TGG | - | chr4.3:89113867-89113886 | None:intergenic | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr4.3 | gene | 89113722 | 89114668 | 89113722 | ID=MS.gene34318 |
| chr4.3 | mRNA | 89113722 | 89114668 | 89113722 | ID=MS.gene34318.t1;Parent=MS.gene34318 |
| chr4.3 | exon | 89113722 | 89113872 | 89113722 | ID=MS.gene34318.t1.exon1;Parent=MS.gene34318.t1 |
| chr4.3 | CDS | 89113722 | 89113872 | 89113722 | ID=cds.MS.gene34318.t1;Parent=MS.gene34318.t1 |
| chr4.3 | exon | 89114165 | 89114301 | 89114165 | ID=MS.gene34318.t1.exon2;Parent=MS.gene34318.t1 |
| chr4.3 | CDS | 89114165 | 89114301 | 89114165 | ID=cds.MS.gene34318.t1;Parent=MS.gene34318.t1 |
| chr4.3 | exon | 89114501 | 89114668 | 89114501 | ID=MS.gene34318.t1.exon3;Parent=MS.gene34318.t1 |
| chr4.3 | CDS | 89114501 | 89114668 | 89114501 | ID=cds.MS.gene34318.t1;Parent=MS.gene34318.t1 |
| Gene Sequence |
| Protein sequence |
