AlfalfaGEDB | MS.gene34340

M. sativa cultivar XinJiangDaYe / MS.gene34340

KEGG: -
GO: GO:0006810 GO:0006869 GO:0008150 GO:0051179 GO:0051234 GO:0071702
PFAM: PF14368.9

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene34340.t1	XP_013447476.1	94.9	98	5	0	1	98	1	98	2.10E-47	198

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene34340.t1	P82353	67.6	68	22	0	31	98	1	68	4.7e-24	111.7

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene34340.t1	A0A072TVT5	94.9	98	5	0	1	98	1	98	1.5e-47	198.0

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene049652	MS.gene34340	0.832808	7.65E-56	-1.69E-46
MS.gene050780	MS.gene34340	0.807529	4.83E-50	-1.69E-46
MS.gene051490	MS.gene34340	0.801104	1.05E-48	-1.69E-46
MS.gene051492	MS.gene34340	0.823011	1.74E-53	-1.69E-46
MS.gene051744	MS.gene34340	0.819905	9.09E-53	-1.69E-46
MS.gene051831	MS.gene34340	0.800665	1.30E-48	-1.69E-46
MS.gene051832	MS.gene34340	0.808171	3.53E-50	-1.69E-46
MS.gene051975	MS.gene34340	0.806521	7.90E-50	-1.69E-46
MS.gene052889	MS.gene34340	0.806949	6.41E-50	-1.69E-46
MS.gene053094	MS.gene34340	0.951508	1.76E-109	-1.69E-46
MS.gene053340	MS.gene34340	0.822174	2.73E-53	-1.69E-46
MS.gene053963	MS.gene34340	0.828727	7.66E-55	-1.69E-46
MS.gene05410	MS.gene34340	0.817844	2.67E-52	-1.69E-46
MS.gene054576	MS.gene34340	0.822317	2.53E-53	-1.69E-46
MS.gene05469	MS.gene34340	0.818239	2.17E-52	-1.69E-46
MS.gene054892	MS.gene34340	0.822818	1.93E-53	-1.69E-46
MS.gene054893	MS.gene34340	0.820406	6.98E-53	-1.69E-46
MS.gene055147	MS.gene34340	0.84848	5.97E-60	-1.69E-46
MS.gene055148	MS.gene34340	0.83277	7.82E-56	-1.69E-46
MS.gene055149	MS.gene34340	0.802999	4.30E-49	-1.69E-46
MS.gene055226	MS.gene34340	0.810721	1.00E-50	-1.69E-46
MS.gene056699	MS.gene34340	0.814281	1.67E-51	-1.69E-46
MS.gene056986	MS.gene34340	0.814025	1.90E-51	-1.69E-46
MS.gene058592	MS.gene34340	0.80306	4.17E-49	-1.69E-46
MS.gene059774	MS.gene34340	0.842433	2.59E-58	-1.69E-46
MS.gene05985	MS.gene34340	-0.820202	7.77E-53	-1.69E-46
MS.gene060412	MS.gene34340	0.832127	1.13E-55	-1.69E-46
MS.gene060697	MS.gene34340	0.839419	1.60E-57	-1.69E-46
MS.gene060840	MS.gene34340	0.804869	1.76E-49	-1.69E-46
MS.gene061047	MS.gene34340	0.812523	4.06E-51	-1.69E-46

PPI:

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene34340.t1	MTR_7g007440	94.898	98	5	1	98	1	98	3.54e-56	169
MS.gene34340.t1	MTR_7g082640	53.933	89	41	10	98	6	94	2.99e-35	115
MS.gene34340.t1	MTR_6g082480	49.383	81	41	18	98	12	92	4.97e-29	100
MS.gene34340.t1	MTR_1g109650	42.222	90	52	9	98	3	92	1.54e-26	94.0
MS.gene34340.t1	MTR_7g082590	42.045	88	51	10	97	6	93	1.81e-22	83.6

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene34340.t1	AT3G18280	49.438	89	42	1	10	98	35	120	2.35e-30	104
MS.gene34340.t1	AT3G18280	49.438	89	42	1	10	98	11	96	4.03e-30	103
MS.gene34340.t1	AT1G48750	55.556	72	32	0	27	98	23	94	1.41e-28	99.4
MS.gene34340.t1	AT1G73780	34.694	98	64	0	1	98	1	98	2.67e-22	83.6
MS.gene34340.t1	AT1G66850	50.725	69	34	0	30	98	34	102	1.95e-21	81.3
MS.gene34340.t1	AT1G43666	46.154	91	43	3	8	98	11	95	3.68e-20	77.8
MS.gene34340.t1	AT5G38170	45.588	68	37	0	31	98	36	103	4.46e-19	75.5
MS.gene34340.t1	AT5G38195	47.761	67	35	0	32	98	29	95	8.84e-19	74.3
MS.gene34340.t1	AT1G43667	35.000	100	61	2	1	98	1	98	1.80e-18	73.6
MS.gene34340.t1	AT5G38160	40.260	77	42	1	22	98	31	103	9.40e-18	72.0
MS.gene34340.t1	AT5G38180	36.000	100	57	2	1	98	1	95	1.05e-17	71.6
MS.gene34340.t1	AT2G14846	45.161	62	34	0	37	98	38	99	1.26e-14	63.9
MS.gene34340.t1	AT1G43665	36.250	80	45	2	17	96	22	95	2.21e-14	63.9
MS.gene34340.t1	AT1G07747	40.909	66	37	1	33	98	29	92	2.22e-13	60.8
MS.gene34340.t1	AT5G38197	34.694	98	62	2	1	98	1	96	4.46e-13	60.1
MS.gene34340.t1	AT3G57310	36.364	66	42	0	33	98	38	103	1.02e-12	59.3
MS.gene34340.t1	AT4G12825	34.066	91	50	2	16	98	7	95	1.62e-12	58.5

Find 28 sgRNAs with CRISPR-Local

Find 30 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
GTAGAGTTCAGTCCTTGTTT+AGG	0.334416	6.3:-343527	MS.gene34340:CDS
GTCTCTGCTCTCTTAACTTC+TGG	0.337118	6.3:+343465	None:intergenic
TTTACTCTCCTGGTGCTAGA+AGG	0.339135	6.3:-343397	MS.gene34340:CDS
GAGCAGAGACCATGCCTTTG+TGG	0.411218	6.3:-343452	MS.gene34340:CDS
GAAGGGTTACTAGCTCTTGT+GGG	0.413579	6.3:-343379	MS.gene34340:CDS
TTCTGGCAACAAGCACTTGT+TGG	0.433827	6.3:+343482	None:intergenic
ATGATTGATGGCTAACAAGT+TGG	0.442427	6.3:+343347	None:intergenic
TAAATATACTGCTTGAGGCT+TGG	0.456606	6.3:+343416	None:intergenic
AAACAAGGACTGAACTCTAC+AGG	0.466330	6.3:+343530	None:intergenic
TGGCAACAAGCACTTGTTGG+TGG	0.497936	6.3:+343485	None:intergenic
AGAAGGGTTACTAGCTCTTG+TGG	0.498825	6.3:-343380	MS.gene34340:CDS
TGATTGATGGCTAACAAGTT+GGG	0.507677	6.3:+343348	None:intergenic
TGTTTATGCAGTAGTGGTTG+TGG	0.508459	6.3:-343606	MS.gene34340:CDS
AAGCAGTATATTTACTCTCC+TGG	0.517554	6.3:-343407	MS.gene34340:CDS
TGGTATTGTTTATGCAGTAG+TGG	0.524181	6.3:-343612	MS.gene34340:CDS
CTAGTAACCCTTCTAGCACC+AGG	0.531212	6.3:+343389	None:intergenic
TTACTCTCCTGGTGCTAGAA+GGG	0.532631	6.3:-343396	MS.gene34340:CDS
GCTTGAGGCTTGGATTCTTG+AGG	0.548134	6.3:+343426	None:intergenic
ATGAAGAAACACTCTAGCTA+TGG	0.561126	6.3:-343632	MS.gene34340:CDS
TGATGGCTAACAAGTTGGGA+AGG	0.576643	6.3:+343352	None:intergenic
GAGAGTAAATATACTGCTTG+AGG	0.582416	6.3:+343411	None:intergenic
AAGGGTTACTAGCTCTTGTG+GGG	0.597115	6.3:-343378	MS.gene34340:CDS
GGAGGAGACTTACATGGCAA+AGG	0.624840	6.3:-343567	MS.gene34340:CDS
AACAAGGACTGAACTCTACA+GGG	0.629235	6.3:+343531	None:intergenic
GATGGCTAACAAGTTGGGAA+GGG	0.649759	6.3:+343353	None:intergenic
TTGAGGTAACCACAAAGGCA+TGG	0.650224	6.3:+343443	None:intergenic
GATTCTTGAGGTAACCACAA+AGG	0.669776	6.3:+343438	None:intergenic
GTTGTGATTGATCCTAAACA+AGG	0.684294	6.3:+343515	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
	AAGCAGTATATTTACTCTCC+TGG	-	chr6.3:343583-343602	MS.gene34340:CDS	35.0%
	ATGAAGAAACACTCTAGCTA+TGG	-	chr6.3:343358-343377	MS.gene34340:CDS	35.0%
	GAGAGTAAATATACTGCTTG+AGG	+	chr6.3:343582-343601	None:intergenic	35.0%
	GTTGTGATTGATCCTAAACA+AGG	+	chr6.3:343478-343497	None:intergenic	35.0%
	TAAATATACTGCTTGAGGCT+TGG	+	chr6.3:343577-343596	None:intergenic	35.0%
	TGGTATTGTTTATGCAGTAG+TGG	-	chr6.3:343378-343397	MS.gene34340:CDS	35.0%
!	GTTGTGGCAATAGTGTTTTT+GGG	-	chr6.3:343400-343419	MS.gene34340:CDS	35.0%
!!	TTGTGGCAATAGTGTTTTTG+GGG	-	chr6.3:343401-343420	MS.gene34340:CDS	35.0%
	AAACAAGGACTGAACTCTAC+AGG	+	chr6.3:343463-343482	None:intergenic	40.0%
	AACAAGGACTGAACTCTACA+GGG	+	chr6.3:343462-343481	None:intergenic	40.0%
	GATTCTTGAGGTAACCACAA+AGG	+	chr6.3:343555-343574	None:intergenic	40.0%
	TGTTTATGCAGTAGTGGTTG+TGG	-	chr6.3:343384-343403	MS.gene34340:CDS	40.0%
!	GGTTGTGGCAATAGTGTTTT+TGG	-	chr6.3:343399-343418	MS.gene34340:CDS	40.0%
!	GTAGAGTTCAGTCCTTGTTT+AGG	-	chr6.3:343463-343482	MS.gene34340:CDS	40.0%
!!!	TGTGGCAATAGTGTTTTTGG+GGG	-	chr6.3:343402-343421	MS.gene34340:CDS	40.0%
	GTCTCTGCTCTCTTAACTTC+TGG	+	chr6.3:343528-343547	None:intergenic	45.0%
	TTCTGGCAACAAGCACTTGT+TGG	+	chr6.3:343511-343530	None:intergenic	45.0%
	TTGAGGTAACCACAAAGGCA+TGG	+	chr6.3:343550-343569	None:intergenic	45.0%
!	AAGGGTTACTAGCTCTTGTG+GGG	-	chr6.3:343612-343631	MS.gene34340:CDS	45.0%
!	AGAAGGGTTACTAGCTCTTG+TGG	-	chr6.3:343610-343629	MS.gene34340:CDS	45.0%
!	GAAGGGTTACTAGCTCTTGT+GGG	-	chr6.3:343611-343630	MS.gene34340:CDS	45.0%
!	TTACTCTCCTGGTGCTAGAA+GGG	-	chr6.3:343594-343613	MS.gene34340:CDS	45.0%
!	TTTACTCTCCTGGTGCTAGA+AGG	-	chr6.3:343593-343612	MS.gene34340:CDS	45.0%
	CTAGTAACCCTTCTAGCACC+AGG	+	chr6.3:343604-343623	None:intergenic	50.0%
	GCTTGAGGCTTGGATTCTTG+AGG	+	chr6.3:343567-343586	None:intergenic	50.0%
	GGAGGAGACTTACATGGCAA+AGG	-	chr6.3:343423-343442	MS.gene34340:CDS	50.0%
	TTTGGGGGAGGAGACTTACA+TGG	-	chr6.3:343417-343436	MS.gene34340:CDS	50.0%
!	TGGCAACAAGCACTTGTTGG+TGG	+	chr6.3:343508-343527	None:intergenic	50.0%
!!!	GGCAATAGTGTTTTTGGGGG+AGG	-	chr6.3:343405-343424	MS.gene34340:CDS	50.0%
	GAGCAGAGACCATGCCTTTG+TGG	-	chr6.3:343538-343557	MS.gene34340:CDS	55.0%

Chromosome	Type	Strat	End	Strand	Name
chr6.3	gene	343358	343654	343358	ID=MS.gene34340
chr6.3	mRNA	343358	343654	343358	ID=MS.gene34340.t1;Parent=MS.gene34340
chr6.3	exon	343358	343654	343358	ID=MS.gene34340.t1.exon1;Parent=MS.gene34340.t1
chr6.3	CDS	343358	343654	343358	ID=cds.MS.gene34340.t1;Parent=MS.gene34340.t1

Gene Sequence	>MS.gene34340 ATGAAGAAACACTCTAGCTATGGTATTGTTTATGCAGTAGTGGTTGTGGCAATAGTGTTTTTGGGGGAGGAGACTTACATGGCAAAGGCATTGACTTGTAGCCCTGTAGAGTTCAGTCCTTGTTTAGGATCAATCACAACTTCTTCTCCACCAACAAGTGCTTGTTGCCAGAAGTTAAGAGAGCAGAGACCATGCCTTTGTGGTTACCTCAAGAATCCAAGCCTCAAGCAGTATATTTACTCTCCTGGTGCTAGAAGGGTTACTAGCTCTTGTGGGGTTCCCTTCCCAACTTGTTAG
Protein sequence	>MS.gene34340.t1 MKKHSSYGIVYAVVVVAIVFLGEETYMAKALTCSPVEFSPCLGSITTSSPPTSACCQKLREQRPCLCGYLKNPSLKQYIYSPGARRVTSSCGVPFPTC