AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene35518

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene35518.t1 MTR_2g087660 99.520 417 2 0 1 417 1 417 0.0 858
MS.gene35518.t1 MTR_4g050420 91.169 419 32 3 1 417 1 416 0.0 686
MS.gene35518.t1 MTR_2g087660 98.726 314 4 0 1 314 1 314 0.0 639
MS.gene35518.t1 MTR_8g006430 70.991 424 115 5 1 417 1 423 0.0 582
MS.gene35518.t1 MTR_7g109340 37.322 351 180 9 14 358 27 343 2.80e-58 194
MS.gene35518.t1 MTR_4g078850 33.333 360 204 10 14 358 5 343 9.01e-51 174
MS.gene35518.t1 MTR_2g104030 32.143 364 221 11 11 357 68 422 3.68e-49 174
MS.gene35518.t1 MTR_4g023470 34.463 354 186 11 14 347 87 414 1.60e-48 171
MS.gene35518.t1 MTR_3g087030 33.144 353 217 9 14 357 83 425 3.15e-48 170
MS.gene35518.t1 MTR_2g090705 32.234 394 154 9 14 358 5 334 3.02e-47 165
MS.gene35518.t1 MTR_2g014470 33.333 360 199 11 14 358 5 338 4.81e-47 164
MS.gene35518.t1 MTR_3g108020 32.213 357 194 7 14 358 5 325 5.63e-46 161
MS.gene35518.t1 MTR_2g014480 34.018 341 199 8 14 343 5 330 2.07e-45 160
MS.gene35518.t1 MTR_4g015840 30.151 398 161 9 14 358 5 338 1.01e-39 145
MS.gene35518.t1 MTR_3g027580 30.645 372 212 15 1 355 80 422 5.67e-39 145
MS.gene35518.t1 MTR_6g471050 30.792 341 210 11 41 365 9 339 1.10e-37 142
MS.gene35518.t1 MTR_5g065160 27.660 376 225 11 1 355 70 419 6.33e-32 126
MS.gene35518.t1 MTR_5g065160 27.957 372 221 11 5 355 75 420 2.28e-31 124
MS.gene35518.t1 MTR_5g065160 27.957 372 221 11 5 355 68 413 3.72e-31 124
MS.gene35518.t1 MTR_4g023490 31.781 365 209 14 14 358 17 361 5.04e-31 122
MS.gene35518.t1 MTR_5g065160 27.957 372 221 11 5 355 75 420 5.36e-31 124
MS.gene35518.t1 MTR_5g037880 36.552 145 90 1 214 358 132 274 7.79e-28 111
MS.gene35518.t1 MTR_5g037880 32.215 149 63 5 11 146 3 126 7.92e-11 62.8
MS.gene35518.t1 MTR_4g015840 36.232 138 86 1 221 358 126 261 1.08e-24 102
MS.gene35518.t1 MTR_3g437840 34.752 141 90 1 218 358 23 161 4.21e-23 95.5
MS.gene35518.t1 MTR_3g088140 34.483 145 92 2 215 358 211 353 8.31e-19 87.4
MS.gene35518.t1 MTR_2g006890 32.639 144 96 1 215 358 233 375 8.77e-18 84.7
MS.gene35518.t1 MTR_3g437850 30.723 166 78 5 14 147 3 163 2.60e-16 76.6
MS.gene35518.t1 MTR_8g037810 46.392 97 43 3 12 104 5 96 3.18e-15 76.6
MS.gene35518.t1 MTR_1g029460 43.878 98 44 4 12 104 5 96 3.39e-14 74.3
MS.gene35518.t1 MTR_5g012530 43.750 80 38 1 10 82 9 88 4.81e-14 74.3
MS.gene35518.t1 MTR_7g101070 52.564 78 31 2 4 76 349 425 5.06e-14 73.9
MS.gene35518.t1 MTR_1g110310 52.941 68 28 1 15 78 18 85 1.07e-13 72.8
MS.gene35518.t1 MTR_1g029470 43.878 98 44 4 12 104 5 96 2.80e-13 71.2
MS.gene35518.t1 MTR_5g004570 40.206 97 49 3 12 104 5 96 1.26e-12 67.8
MS.gene35518.t1 MTR_3g437360 30.534 131 88 3 210 339 206 334 2.89e-12 67.8
MS.gene35518.t1 MTR_5g035770 37.255 102 50 3 15 105 27 125 1.49e-11 66.2
MS.gene35518.t1 MTR_7g112360 40.000 75 37 1 14 80 17 91 4.74e-11 61.6
MS.gene35518.t1 MTR_7g112360 40.000 75 37 1 14 80 17 91 6.38e-11 59.7
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene35518.t1 AT5G22060 83.055 419 69 2 1 417 1 419 0.0 684
MS.gene35518.t1 AT3G44110 85.036 421 58 3 1 417 1 420 0.0 655
MS.gene35518.t1 AT3G44110 87.651 332 39 2 1 331 1 331 0.0 530
MS.gene35518.t1 AT3G62600 35.775 355 182 11 14 358 27 345 7.55e-56 188
MS.gene35518.t1 AT1G80030 32.964 361 216 10 14 357 76 427 5.33e-53 184
MS.gene35518.t1 AT1G80030 32.964 361 216 10 14 357 76 427 5.33e-53 184
MS.gene35518.t1 AT1G80030 32.964 361 216 10 14 357 76 427 5.33e-53 184
MS.gene35518.t1 AT1G80030 32.964 361 216 10 14 357 76 427 6.73e-53 184
MS.gene35518.t1 AT2G20560 33.711 353 205 8 14 358 5 336 8.01e-50 172
MS.gene35518.t1 AT5G25530 32.597 362 198 9 14 358 5 337 9.08e-48 166
MS.gene35518.t1 AT5G48030 34.167 360 201 13 14 355 95 436 1.02e-47 169
MS.gene35518.t1 AT2G22360 32.075 371 229 10 14 378 87 440 5.65e-47 167
MS.gene35518.t1 AT4G39960 31.198 359 228 9 11 357 83 434 5.94e-45 162
MS.gene35518.t1 AT4G39960 31.198 359 228 9 11 357 83 434 5.94e-45 162
MS.gene35518.t1 AT5G25530 31.892 370 198 10 14 358 5 345 6.69e-45 159
MS.gene35518.t1 AT4G28480 31.198 359 217 8 14 358 5 347 1.25e-44 158
MS.gene35518.t1 AT3G08910 30.789 380 164 9 14 358 5 320 9.36e-40 145
MS.gene35518.t1 AT3G17830 30.303 363 225 10 12 355 62 415 5.31e-38 144
MS.gene35518.t1 AT5G01390 29.517 393 165 11 14 358 5 333 9.13e-38 140
MS.gene35518.t1 AT1G28210 27.895 380 229 11 7 368 42 394 1.06e-33 130
MS.gene35518.t1 AT1G28210 27.895 380 229 11 7 368 42 394 1.48e-33 130
MS.gene35518.t1 AT1G28210 27.895 380 229 11 7 368 42 394 1.81e-33 130
MS.gene35518.t1 AT2G20550 37.241 145 89 1 214 358 141 283 1.05e-26 108
MS.gene35518.t1 AT2G20550 37.241 145 89 1 214 358 141 283 1.05e-26 108
MS.gene35518.t1 AT3G47940 36.552 145 90 1 214 358 204 346 1.79e-26 109
MS.gene35518.t1 AT3G47940 34.021 194 67 4 14 146 5 198 1.11e-20 93.2
MS.gene35518.t1 AT5G01390 35.507 138 87 1 221 358 140 275 1.09e-23 100
MS.gene35518.t1 AT5G01390 40.741 108 43 3 14 100 5 112 1.77e-16 79.7
MS.gene35518.t1 AT4G28480 34.694 147 92 2 212 358 147 289 1.66e-23 100
MS.gene35518.t1 AT4G28480 43.750 112 50 2 14 120 5 108 5.99e-18 84.3
MS.gene35518.t1 AT1G59725 36.957 138 85 1 221 358 193 328 7.36e-23 99.0
MS.gene35518.t1 AT1G59725 32.955 176 75 4 14 146 5 180 4.28e-19 88.2
MS.gene35518.t1 AT1G10350 36.552 145 87 3 214 358 207 346 7.85e-23 99.4
MS.gene35518.t1 AT1G10350 32.308 195 70 6 14 146 5 199 3.66e-19 88.6
MS.gene35518.t1 AT5G01390 35.870 184 70 6 14 149 5 188 7.41e-22 94.0
MS.gene35518.t1 AT1G11040 37.500 136 83 1 215 350 291 424 3.99e-20 92.4
MS.gene35518.t1 AT1G44160 32.593 135 89 1 224 358 221 353 1.69e-17 84.0
MS.gene35518.t1 AT5G01390 40.741 108 43 3 14 100 5 112 9.65e-17 79.7
MS.gene35518.t1 AT2G21510 44.330 97 45 3 12 104 5 96 1.78e-15 77.4
MS.gene35518.t1 AT2G21510 44.330 97 45 3 12 104 5 96 2.48e-15 77.4
MS.gene35518.t1 AT2G21510 44.330 97 45 3 12 104 5 96 2.48e-15 77.4
MS.gene35518.t1 AT2G21510 44.330 97 45 3 12 104 5 96 2.55e-15 77.4
MS.gene35518.t1 AT2G21510 44.330 97 45 3 12 104 5 96 2.55e-15 77.4
MS.gene35518.t1 AT5G05750 41.748 103 46 3 14 102 115 217 4.24e-14 72.8
MS.gene35518.t1 AT4G39150 42.268 97 47 3 12 104 5 96 9.40e-14 72.4
MS.gene35518.t1 AT4G39150 42.268 97 47 3 12 104 5 96 9.40e-14 72.4
MS.gene35518.t1 AT4G39150 42.268 97 47 3 12 104 5 96 9.40e-14 72.4
MS.gene35518.t1 AT1G59980 47.059 68 32 1 15 78 25 92 4.21e-13 70.5
MS.gene35518.t1 AT1G59980 47.059 68 32 1 15 78 25 92 6.33e-13 70.5
MS.gene35518.t1 AT5G03160 50.000 94 40 4 4 92 361 452 1.17e-12 69.7
MS.gene35518.t1 AT1G68370 51.471 68 29 1 15 78 19 86 1.24e-12 69.7
MS.gene35518.t1 AT3G57340 42.424 99 42 3 14 97 114 212 1.44e-12 68.9
MS.gene35518.t1 AT3G57340 42.424 99 42 3 14 97 114 212 1.44e-12 68.9
MS.gene35518.t1 AT3G08970 47.297 74 36 1 15 85 29 102 1.72e-12 69.7
MS.gene35518.t1 AT3G08970 47.297 74 36 1 15 85 29 102 1.72e-12 69.7
MS.gene35518.t1 AT1G24120 37.255 102 50 4 15 105 22 120 3.92e-12 68.2
MS.gene35518.t1 AT3G12170 46.479 71 34 1 8 74 42 112 1.25e-11 65.5
MS.gene35518.t1 AT3G12170 31.707 164 84 6 8 166 6 146 1.25e-11 65.1
MS.gene35518.t1 AT1G21080 39.175 97 49 3 12 104 5 95 2.06e-11 65.9
MS.gene35518.t1 AT1G21080 39.175 97 49 3 12 104 5 95 2.18e-11 65.5
MS.gene35518.t1 AT2G35720 38.824 85 45 1 5 82 7 91 2.74e-11 65.9
MS.gene35518.t1 AT1G74250 44.928 69 29 2 14 73 12 80 3.77e-11 65.5
MS.gene35518.t1 AT5G49060 46.269 67 33 1 11 74 97 163 3.98e-11 64.7
MS.gene35518.t1 AT5G49060 46.269 67 33 1 11 74 97 163 3.98e-11 64.7
MS.gene35518.t1 AT1G76700 39.394 99 51 3 12 106 5 98 6.16e-11 64.3
MS.gene35518.t1 AT4G10130 43.939 66 30 1 14 72 12 77 6.19e-11 61.6
MS.gene35518.t1 AT4G10130 43.939 66 30 1 14 72 12 77 6.19e-11 61.6
MS.gene35518.t1 AT4G10130 43.939 66 30 1 14 72 12 77 6.19e-11 61.6
MS.gene35518.t1 AT4G10130 43.939 66 30 1 14 72 12 77 6.19e-11 61.6
MS.gene35518.t1 AT4G10130 43.939 66 30 1 14 72 12 77 6.19e-11 61.6
MS.gene35518.t1 AT1G56300 41.176 68 32 1 12 71 12 79 7.82e-11 60.8
MS.gene35518.t1 AT5G06910 45.714 70 34 1 9 74 25 94 9.17e-11 62.8

Find 119 sgRNAs with CRISPR-Local

Find 190 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTCGGTGGAGGTGGATTTCC+TGG 0.267655 2.4:+13254385 MS.gene35518:CDS
TCACCCCTTGAAGGTCTCTT+TGG 0.287702 2.4:+13254557 MS.gene35518:CDS
CCAATTTGCGCTGACTCATT+TGG 0.293928 2.4:+13255226 MS.gene35518:CDS
GGAATGGGTGGTGGCGGTGG+TGG 0.294243 2.4:+13254331 MS.gene35518:CDS
TCAGGATCACCACCCTTATC+AGG 0.295880 2.4:-13253584 None:intergenic
CCAAATGAGTCAGCGCAAAT+TGG 0.320936 2.4:-13255226 None:intergenic
TTCACCCTTTCGTTTGAACT+TGG 0.337178 2.4:-13255153 None:intergenic
GAAGATATCTATGAGGCATT+TGG 0.338997 2.4:+13254795 MS.gene35518:CDS
GAAGGAATGGGTGGTGGCGG+TGG 0.340853 2.4:+13254328 MS.gene35518:CDS
ACTAAATACTATGAGATTCT+TGG 0.343941 2.4:+13253494 MS.gene35518:CDS
AACTGAGATACAGGCCTTGC+TGG 0.344090 2.4:-13255546 None:intergenic
CCCCTTGAAGGTCTCTTTGG+AGG 0.348117 2.4:+13254560 MS.gene35518:CDS
TTGCAGCCGGACACAGTTAC+TGG 0.350028 2.4:+13255095 MS.gene35518:intron
CACCACCCATTCCTTCCTTA+AGG 0.353366 2.4:-13254321 None:intergenic
TTAGTGTTATCACTCTTCTT+AGG 0.362823 2.4:-13253476 None:intergenic
TGTGCTGGTTGTCAAGGTTC+TGG 0.368082 2.4:+13254769 MS.gene35518:CDS
TCTTGATCAGGTCAAGGCTT+TGG 0.379949 2.4:+13255512 MS.gene35518:CDS
TTGCATTTGCTGAATCATAT+TGG 0.385235 2.4:-13254821 None:intergenic
ACCACCCATTCCTTCCTTAA+GGG 0.391516 2.4:-13254320 None:intergenic
TACGCTTTCTTCAAATCATC+GGG 0.394989 2.4:-13253536 None:intergenic
CACCCTTTCGTTTGAACTTG+GGG 0.398325 2.4:-13255151 None:intergenic
TCACCCTTTCGTTTGAACTT+GGG 0.404206 2.4:-13255152 None:intergenic
AAGATATCTATGAGGCATTT+GGG 0.411623 2.4:+13254796 MS.gene35518:CDS
ATGAAAGGCCTCTGGTACAT+TGG 0.411721 2.4:-13255438 None:intergenic
TTGTGAAATTTGAAGATGTT+TGG 0.412460 2.4:+13253446 None:intergenic
TGAGTCTCTGTCTCTTGATC+AGG 0.415051 2.4:+13255500 MS.gene35518:CDS
ACATCCTTGCAATGAATGTA+AGG 0.418761 2.4:+13254843 MS.gene35518:CDS
CTTTGGAGGATCTTTACCTT+GGG 0.422824 2.4:+13254574 MS.gene35518:CDS
AGAGCTTGCACAAGCTTATG+AGG 0.426543 2.4:+13254246 MS.gene35518:CDS
ATGTCTGTCAACTGAGATAC+AGG 0.432545 2.4:-13255555 None:intergenic
AAAGATGAAAAGATGTCAAA+TGG 0.433471 2.4:-13254361 None:intergenic
CCTTCTCTCCCTTGCACTGT+GGG 0.438316 2.4:-13254900 None:intergenic
TCATATACCTCACGCTTCTC+AGG 0.445588 2.4:-13254280 None:intergenic
TCCCTCACTGAGGCTTTATG+TGG 0.451630 2.4:+13255200 MS.gene35518:CDS
TGCAATGAATGTAAGGGTAC+TGG 0.456888 2.4:+13254850 MS.gene35518:CDS
TCATCTTTCTTCGGTGGAGG+TGG 0.461355 2.4:+13254376 MS.gene35518:CDS
GTATGGTGAAGATGCCCTTA+AGG 0.462316 2.4:+13254306 MS.gene35518:CDS
GGTTTGATTTAATCAATAGT+TGG 0.465413 2.4:-13255256 None:intergenic
ATACGCTTTCTTCAAATCAT+CGG 0.468985 2.4:-13253537 None:intergenic
CGTGAGGTATATGATCAGTA+TGG 0.471347 2.4:+13254289 MS.gene35518:CDS
TCTTTGGAGGATCTTTACCT+TGG 0.472410 2.4:+13254573 MS.gene35518:CDS
GATTAAGAATCATCCTGATA+AGG 0.476346 2.4:+13253571 MS.gene35518:CDS
GGATGTCGTTCACCCCTTGA+AGG 0.478745 2.4:+13254548 MS.gene35518:CDS
AGATCGATGCCCACAGTGCA+AGG 0.481792 2.4:+13254891 MS.gene35518:CDS
ATTAAGAATCATCCTGATAA+GGG 0.485570 2.4:+13253572 MS.gene35518:CDS
TACAGGAAAGGATCAAAGTC+TGG 0.495336 2.4:+13254733 MS.gene35518:intron
TGCCCTTCATGAAAGGCCTC+TGG 0.498481 2.4:-13255446 None:intergenic
CCAGGCTTGACAACTTCTCC+AGG 0.499453 2.4:-13255278 None:intergenic
ATATCTCCAGTAACTGTGTC+CGG 0.501009 2.4:-13255101 None:intergenic
GGTGCTTCGATGACCTGTGC+TGG 0.505989 2.4:+13254754 MS.gene35518:CDS
CTTGAAGTTCATGTTGAGAA+GGG 0.510818 2.4:+13254943 MS.gene35518:CDS
GCATCGATCTTTGTCACTGA+TGG 0.515086 2.4:-13254878 None:intergenic
ATCCTCCAAAGAGACCTTCA+AGG 0.518323 2.4:-13254562 None:intergenic
AAGGAAGGAATGGGTGGTGG+CGG 0.520461 2.4:+13254325 MS.gene35518:CDS
TACAAGGCAATCAATGATGA+AGG 0.521364 2.4:+13255411 MS.gene35518:CDS
CACCCCAAGTTCAAACGAAA+GGG 0.528668 2.4:+13255149 MS.gene35518:CDS
AGATGCCCTTAAGGAAGGAA+TGG 0.532671 2.4:+13254315 MS.gene35518:CDS
TGATCAAGAGACAGAGACTC+AGG 0.533617 2.4:-13255498 None:intergenic
CCTGGAGAAGTTGTCAAGCC+TGG 0.534032 2.4:+13255278 MS.gene35518:CDS
GGTCAGAAGATTACCTTCCC+TGG 0.534089 2.4:+13254976 MS.gene35518:CDS
TTCATCAGCTTCACCAGGGA+AGG 0.534704 2.4:-13254989 None:intergenic
TAACACATACCCCTTGCACT+TGG 0.540192 2.4:-13254637 None:intergenic
ACTTGAAGTTCATGTTGAGA+AGG 0.543623 2.4:+13254942 MS.gene35518:CDS
CTTAAGGAAGGAATGGGTGG+TGG 0.547958 2.4:+13254322 MS.gene35518:CDS
TGTCTTGTGCTCCAAGTGCA+AGG 0.549478 2.4:+13254626 MS.gene35518:CDS
TACCAGAGGCCTTTCATGAA+GGG 0.550239 2.4:+13255444 MS.gene35518:CDS
TCTGTCTCTTGATCAGGTCA+AGG 0.553837 2.4:+13255506 MS.gene35518:CDS
GATGCCCTTAAGGAAGGAAT+GGG 0.556456 2.4:+13254316 MS.gene35518:CDS
CCCACAGTGCAAGGGAGAGA+AGG 0.557002 2.4:+13254900 MS.gene35518:CDS
CGTCTTTGTACTTCAACAGA+AGG 0.558455 2.4:+13255124 MS.gene35518:CDS
TTGAAGAAGAGACAAGAAGA+AGG 0.558667 2.4:+13255622 MS.gene35518:CDS
TAAAGCTTGCCCTTCATGAA+AGG 0.559348 2.4:-13255453 None:intergenic
GTCTTGTGCTCCAAGTGCAA+GGG 0.560067 2.4:+13254627 MS.gene35518:CDS
ACCGCTTCATCAGCTTCACC+AGG 0.560539 2.4:-13254994 None:intergenic
ATGACCTGTGCTGGTTGTCA+AGG 0.561075 2.4:+13254763 MS.gene35518:CDS
GTTGAGAAGGGGATGCAGAA+TGG 0.563375 2.4:+13254955 MS.gene35518:CDS
TGGTTACGCAGATTCATACA+AGG 0.564992 2.4:+13255395 MS.gene35518:intron
GTACCAGAGGCCTTTCATGA+AGG 0.566111 2.4:+13255443 MS.gene35518:CDS
ACTGAGATACAGGCCTTGCT+GGG 0.567469 2.4:-13255545 None:intergenic
GGTGAAGATGCCCTTAAGGA+AGG 0.569050 2.4:+13254310 MS.gene35518:CDS
TCAGTTGACAGACATGGAGC+TGG 0.570239 2.4:+13255563 MS.gene35518:CDS
GTGGTAGTAGCAGAGGAAGA+AGG 0.572018 2.4:+13254511 MS.gene35518:CDS
ACAGGTGGTGGTAGTAGCAG+AGG 0.575959 2.4:+13254504 MS.gene35518:intron
CCCTGGTGAAGCTGATGAAG+CGG 0.582550 2.4:+13254993 MS.gene35518:CDS
AGAACCTTGACAACCAGCAC+AGG 0.585983 2.4:-13254767 None:intergenic
TGGAGACAATTCTCCCAGCA+AGG 0.587524 2.4:+13255532 MS.gene35518:CDS
ACACCCCAAGTTCAAACGAA+AGG 0.592574 2.4:+13255148 MS.gene35518:CDS
TGTACTCTTTGAGCACCACC+AGG 0.594168 2.4:-13255687 None:intergenic
TCCTCCAAAGAGACCTTCAA+GGG 0.596017 2.4:-13254561 None:intergenic
TTGAAGTTCATGTTGAGAAG+GGG 0.603095 2.4:+13254944 MS.gene35518:CDS
GTACCCTTACATTCATTGCA+AGG 0.605875 2.4:-13254847 None:intergenic
CAGGCTTGACAACTTCTCCA+GGG 0.612975 2.4:-13255277 None:intergenic
TAAGGGTGGTGATCCTGAAA+AGG 0.613088 2.4:+13253589 MS.gene35518:CDS
TGTATCTCAGTTGACAGACA+TGG 0.616220 2.4:+13255557 MS.gene35518:CDS
GCCCTTAAGGAAGGAATGGG+TGG 0.620209 2.4:+13254319 MS.gene35518:CDS
GATGAGGATGATGAAATGCC+TGG 0.624859 2.4:+13255669 MS.gene35518:CDS
AAGGCAGCAAGCTCAGCAAG+AGG 0.624868 2.4:+13255641 MS.gene35518:CDS
ACCTTCTCTCCCTTGCACTG+TGG 0.630501 2.4:-13254901 None:intergenic
GATCGATGCCCACAGTGCAA+GGG 0.633588 2.4:+13254892 MS.gene35518:CDS
AAAGCTTCTTAGATGTCCCA+AGG 0.634241 2.4:-13254590 None:intergenic
TCTTGTGCTCCAAGTGCAAG+GGG 0.635966 2.4:+13254628 MS.gene35518:CDS
CTATTGATTAAATCAAACCC+TGG 0.637960 2.4:+13255260 MS.gene35518:CDS
AGAGGAAGAAGGCAGAGACG+TGG 0.638556 2.4:+13254522 MS.gene35518:CDS
CATCCTTGCAATGAATGTAA+GGG 0.643345 2.4:+13254844 MS.gene35518:CDS
AAGAAGGCAGAGACGTGGTG+AGG 0.643979 2.4:+13254527 MS.gene35518:CDS
TCAGCAAGAGGCATATGATG+AGG 0.654664 2.4:+13255653 MS.gene35518:CDS
ACCACATAAAGCCTCAGTGA+GGG 0.659830 2.4:-13255201 None:intergenic
CATGGAGCTGGATGAGTGTG+AGG 0.667513 2.4:+13255575 MS.gene35518:CDS
GATCAAGAGACAGAGACTCA+GGG 0.667543 2.4:-13255497 None:intergenic
AAGGAATGCCAATGTACCAG+AGG 0.667557 2.4:+13255430 MS.gene35518:CDS
CTGGTATGAAGATATCTATG+AGG 0.673684 2.4:+13254788 MS.gene35518:CDS
GAGTGATCCTGAGAAGCGTG+AGG 0.676105 2.4:+13254273 MS.gene35518:CDS
AAGAATCATCCTGATAAGGG+TGG 0.677133 2.4:+13253575 MS.gene35518:CDS
CCGCTTCATCAGCTTCACCA+GGG 0.690995 2.4:-13254993 None:intergenic
GCTTTACATTCACTTCACAG+TGG 0.719754 2.4:+13255470 MS.gene35518:CDS
CCTCCAAAGAGACCTTCAAG+GGG 0.727234 2.4:-13254560 None:intergenic
AACCACATAAAGCCTCAGTG+AGG 0.740190 2.4:-13255202 None:intergenic
GCACACATTGTCCCTCACTG+AGG 0.763892 2.4:+13255190 MS.gene35518:CDS
GAGGATGATGAAATGCCTGG+TGG 0.804018 2.4:+13255672 MS.gene35518:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AATTTGAGATTTTTAAATTA+AGG + chr2.4:13253811-13253830 MS.gene35518:intron 10.0%
!!! ATTTGAGATTTTTAAATTAA+GGG + chr2.4:13253812-13253831 MS.gene35518:intron 10.0%
!! ATTAGTTAATTGATGAAATT+AGG + chr2.4:13254186-13254205 MS.gene35518:intron 15.0%
!! ATTGAATTATATAATTGTTG+TGG + chr2.4:13254013-13254032 MS.gene35518:intron 15.0%
!! TTAGTTAATTGATGAAATTA+GGG + chr2.4:13254187-13254206 MS.gene35518:intron 15.0%
!! TTATCTTTGAATTTGATTAT+TGG + chr2.4:13253622-13253641 MS.gene35518:intron 15.0%
!!! AAAAGTGATTTTTTTACTAT+AGG + chr2.4:13253853-13253872 MS.gene35518:intron 15.0%
!!! CTTGTTTGTTATTTTAATTT+AGG + chr2.4:13254416-13254435 MS.gene35518:intron 15.0%
!!! TATTTTAATTTAGGAGATTA+GGG + chr2.4:13254425-13254444 MS.gene35518:intron 15.0%
!!! TTATTTTAATTTAGGAGATT+AGG + chr2.4:13254424-13254443 MS.gene35518:intron 15.0%
!!! TTTGAGATTTTTAAATTAAG+GGG + chr2.4:13253813-13253832 MS.gene35518:intron 15.0%
!!! AATAGTGATTAGTTGAATTT+AGG + chr2.4:13253713-13253732 MS.gene35518:intron 20.0%
!!! AATTGTGATTAGTTAGTTTT+AGG + chr2.4:13254106-13254125 MS.gene35518:intron 20.0%
!!! ATAGTGATTAGTTGAATTTA+GGG + chr2.4:13253714-13253733 MS.gene35518:intron 20.0%
!!! ATTGAATTTGATAATTGTTG+TGG + chr2.4:13253686-13253705 MS.gene35518:intron 20.0%
!!! ATTGTGATTAGTTAGTTTTA+GGG + chr2.4:13254107-13254126 MS.gene35518:intron 20.0%
!!! ATTGTTAGTTGTATTGATAT+TGG + chr2.4:13254061-13254080 MS.gene35518:intron 20.0%
!!! TAAAAAAAAACAACCTTTTC+AGG - chr2.4:13253605-13253624 None:intergenic 20.0%
!!! TTGTTAGTTGTATTGATATT+GGG + chr2.4:13254062-13254081 MS.gene35518:intron 20.0%
! AAAGATGAAAAGATGTCAAA+TGG - chr2.4:13254364-13254383 None:intergenic 25.0%
! ACTAAATACTATGAGATTCT+TGG + chr2.4:13253494-13253513 MS.gene35518:CDS 25.0%
! ATCATATTGTATTTGTGAAC+AGG + chr2.4:13254486-13254505 MS.gene35518:intron 25.0%
! ATTAAGAATCATCCTGATAA+GGG + chr2.4:13253572-13253591 MS.gene35518:CDS 25.0%
! ATTGATTATGCAATTAGGAT+TGG + chr2.4:13253940-13253959 MS.gene35518:intron 25.0%
! ATTGGGTTATATAATTGTTG+TGG + chr2.4:13254079-13254098 MS.gene35518:intron 25.0%
! GATTATTGGATTATCTGATT+TGG + chr2.4:13253636-13253655 MS.gene35518:intron 25.0%
! TTGGTAGAATGATATTTGTT+AGG + chr2.4:13253767-13253786 MS.gene35518:intron 25.0%
!! GGTTTGATTTAATCAATAGT+TGG - chr2.4:13255259-13255278 None:intergenic 25.0%
!! TGCTGAAATTTTCAATCTTT+TGG + chr2.4:13255375-13255394 MS.gene35518:intron 25.0%
!! TGGTAGAATGATATTTGTTA+GGG + chr2.4:13253768-13253787 MS.gene35518:intron 25.0%
!!! GTTTGTTGGTATTTTTCTAA+TGG + chr2.4:13254678-13254697 MS.gene35518:intron 25.0%
!!! TTGGTATTGATTATGCAATT+AGG + chr2.4:13253935-13253954 MS.gene35518:intron 25.0%
AAAATCAAACTACCATAACC+CGG - chr2.4:13254153-13254172 None:intergenic 30.0%
AATATCAATCCCATTAGCTA+CGG - chr2.4:13253883-13253902 None:intergenic 30.0%
ACACCTAACAACAATCAATT+GGG - chr2.4:13253914-13253933 None:intergenic 30.0%
ATACGCTTTCTTCAAATCAT+CGG - chr2.4:13253540-13253559 None:intergenic 30.0%
ATATTGTATTTGTGAACAGG+TGG + chr2.4:13254489-13254508 MS.gene35518:intron 30.0%
ATGTGAATAATCTACATACC+AGG - chr2.4:13255299-13255318 None:intergenic 30.0%
GATTAAGAATCATCCTGATA+AGG + chr2.4:13253571-13253590 MS.gene35518:CDS 30.0%
GTATGTAGATTATTCACATC+TGG + chr2.4:13255300-13255319 MS.gene35518:intron 30.0%
TACACCTAACAACAATCAAT+TGG - chr2.4:13253915-13253934 None:intergenic 30.0%
TTAGTGTTATCACTCTTCTT+AGG - chr2.4:13253479-13253498 None:intergenic 30.0%
TTGTTAGGGTTTATTTGTCA+TGG + chr2.4:13253782-13253801 MS.gene35518:intron 30.0%
TTTGATTGAATTAGGGTTGT+GGG + chr2.4:13253741-13253760 MS.gene35518:intron 30.0%
! AAGATATCTATGAGGCATTT+GGG + chr2.4:13254796-13254815 MS.gene35518:CDS 30.0%
! CTATTGATTAAATCAAACCC+TGG + chr2.4:13255260-13255279 MS.gene35518:CDS 30.0%
! GACATCTTTTCATCTTTCTT+CGG + chr2.4:13254367-13254386 MS.gene35518:CDS 30.0%
! TTACCCAATTGATTGTTGTT+AGG + chr2.4:13253908-13253927 MS.gene35518:intron 30.0%
! TTGATTGTTGTTAGGTGTAT+TGG + chr2.4:13253916-13253935 MS.gene35518:intron 30.0%
! TTGCATTTGCTGAATCATAT+TGG - chr2.4:13254824-13254843 None:intergenic 30.0%
! TTGGTATTTTTCTAATGGCT+CGG + chr2.4:13254683-13254702 MS.gene35518:intron 30.0%
!! AGGGTTAGTTTGATTGAATT+AGG + chr2.4:13253733-13253752 MS.gene35518:intron 30.0%
!! GGGTTAGTTTGATTGAATTA+GGG + chr2.4:13253734-13253753 MS.gene35518:intron 30.0%
!! TTGTTGATTGGTTTGATTAC+AGG + chr2.4:13254716-13254735 MS.gene35518:intron 30.0%
!!! GACTGAATTTTTTTCGTCTT+TGG + chr2.4:13255352-13255371 MS.gene35518:intron 30.0%
AAATCAAACTACCATAACCC+GGG - chr2.4:13254152-13254171 None:intergenic 35.0%
AACAAACAAGAAACCATACC+AGG - chr2.4:13254406-13254425 None:intergenic 35.0%
ACATCCTTGCAATGAATGTA+AGG + chr2.4:13254843-13254862 MS.gene35518:CDS 35.0%
ACTTGAAGTTCATGTTGAGA+AGG + chr2.4:13254942-13254961 MS.gene35518:CDS 35.0%
AGGGTAGTGTGATTGAATTA+AGG + chr2.4:13254126-13254145 MS.gene35518:intron 35.0%
ATATCAATCCCATTAGCTAC+GGG - chr2.4:13253882-13253901 None:intergenic 35.0%
CATCCTTGCAATGAATGTAA+GGG + chr2.4:13254844-13254863 MS.gene35518:CDS 35.0%
CTGGTATGAAGATATCTATG+AGG + chr2.4:13254788-13254807 MS.gene35518:CDS 35.0%
CTTGAAGTTCATGTTGAGAA+GGG + chr2.4:13254943-13254962 MS.gene35518:CDS 35.0%
GGAGTTCTTACATTGTTGAT+TGG + chr2.4:13254704-13254723 MS.gene35518:intron 35.0%
GTTTGATTGAATTAGGGTTG+TGG + chr2.4:13253740-13253759 MS.gene35518:intron 35.0%
TACAAGGCAATCAATGATGA+AGG + chr2.4:13255411-13255430 MS.gene35518:CDS 35.0%
TACGCTTTCTTCAAATCATC+GGG - chr2.4:13253539-13253558 None:intergenic 35.0%
TTGAAGAAGAGACAAGAAGA+AGG + chr2.4:13255622-13255641 MS.gene35518:CDS 35.0%
TTGAAGTTCATGTTGAGAAG+GGG + chr2.4:13254944-13254963 MS.gene35518:CDS 35.0%
! ATCTTTTCATCTTTCTTCGG+TGG + chr2.4:13254370-13254389 MS.gene35518:CDS 35.0%
! GAAGATATCTATGAGGCATT+TGG + chr2.4:13254795-13254814 MS.gene35518:CDS 35.0%
!! GATTGGTTTGATTACAGGAA+AGG + chr2.4:13254721-13254740 MS.gene35518:intron 35.0%
!! GGGTTATGGTAGTTTGATTT+TGG + chr2.4:13254152-13254171 MS.gene35518:intron 35.0%
AAAGCTTCTTAGATGTCCCA+AGG - chr2.4:13254593-13254612 None:intergenic 40.0%
AAGAATCATCCTGATAAGGG+TGG + chr2.4:13253575-13253594 MS.gene35518:CDS 40.0%
AGGTCTATCTGCATGTTTGT+TGG + chr2.4:13254664-13254683 MS.gene35518:intron 40.0%
AGTGTGATTGAATTAAGGCC+CGG + chr2.4:13254131-13254150 MS.gene35518:intron 40.0%
ATATCTCCAGTAACTGTGTC+CGG - chr2.4:13255104-13255123 None:intergenic 40.0%
ATGTCTGTCAACTGAGATAC+AGG - chr2.4:13255558-13255577 None:intergenic 40.0%
CAACAATCCAGCTCAAACTA+AGG - chr2.4:13255040-13255059 None:intergenic 40.0%
CGTCTTTGTACTTCAACAGA+AGG + chr2.4:13255124-13255143 MS.gene35518:CDS 40.0%
CGTGAGGTATATGATCAGTA+TGG + chr2.4:13254289-13254308 MS.gene35518:CDS 40.0%
CTTATTGTTTGTCTTGCAGC+CGG + chr2.4:13255082-13255101 MS.gene35518:intron 40.0%
GCTTTACATTCACTTCACAG+TGG + chr2.4:13255470-13255489 MS.gene35518:CDS 40.0%
GTACCCTTACATTCATTGCA+AGG - chr2.4:13254850-13254869 None:intergenic 40.0%
TAAAGCTTGCCCTTCATGAA+AGG - chr2.4:13255456-13255475 None:intergenic 40.0%
TACAGGAAAGGATCAAAGTC+TGG + chr2.4:13254733-13254752 MS.gene35518:intron 40.0%
TATCAATCCCATTAGCTACG+GGG - chr2.4:13253881-13253900 None:intergenic 40.0%
TCACCCTTTCGTTTGAACTT+GGG - chr2.4:13255155-13255174 None:intergenic 40.0%
TGGTTACGCAGATTCATACA+AGG + chr2.4:13255395-13255414 MS.gene35518:intron 40.0%
TGTATCTCAGTTGACAGACA+TGG + chr2.4:13255557-13255576 MS.gene35518:CDS 40.0%
TTCACCCTTTCGTTTGAACT+TGG - chr2.4:13255156-13255175 None:intergenic 40.0%
TTGTATTTGTGAACAGGTGG+TGG + chr2.4:13254492-13254511 MS.gene35518:intron 40.0%
! CTTTGGAGGATCTTTACCTT+GGG + chr2.4:13254574-13254593 MS.gene35518:CDS 40.0%
! TACAGTTCCTTAGTTTGAGC+TGG + chr2.4:13255030-13255049 MS.gene35518:intron 40.0%
! TCTTTGGAGGATCTTTACCT+TGG + chr2.4:13254573-13254592 MS.gene35518:CDS 40.0%
! TGCAATGAATGTAAGGGTAC+TGG + chr2.4:13254850-13254869 MS.gene35518:CDS 40.0%
! TTTTCATCTTTCTTCGGTGG+AGG + chr2.4:13254373-13254392 MS.gene35518:CDS 40.0%
AACCACATAAAGCCTCAGTG+AGG - chr2.4:13255205-13255224 None:intergenic 45.0%
AAGGAATGCCAATGTACCAG+AGG + chr2.4:13255430-13255449 MS.gene35518:CDS 45.0%
ACACCCCAAGTTCAAACGAA+AGG + chr2.4:13255148-13255167 MS.gene35518:CDS 45.0%
ACCACATAAAGCCTCAGTGA+GGG - chr2.4:13255204-13255223 None:intergenic 45.0%
ACCACCCATTCCTTCCTTAA+GGG - chr2.4:13254323-13254342 None:intergenic 45.0%
AGAGCTTGCACAAGCTTATG+AGG + chr2.4:13254246-13254265 MS.gene35518:CDS 45.0%
AGATGCCCTTAAGGAAGGAA+TGG + chr2.4:13254315-13254334 MS.gene35518:CDS 45.0%
ATAGGATCCCCGTAGCTAAT+GGG + chr2.4:13253871-13253890 MS.gene35518:intron 45.0%
ATCCTCCAAAGAGACCTTCA+AGG - chr2.4:13254565-13254584 None:intergenic 45.0%
ATGAAAGGCCTCTGGTACAT+TGG - chr2.4:13255441-13255460 None:intergenic 45.0%
CACCCCAAGTTCAAACGAAA+GGG + chr2.4:13255149-13255168 MS.gene35518:CDS 45.0%
CACCCTTTCGTTTGAACTTG+GGG - chr2.4:13255154-13255173 None:intergenic 45.0%
CCAAATGAGTCAGCGCAAAT+TGG - chr2.4:13255229-13255248 None:intergenic 45.0%
GATCAAGAGACAGAGACTCA+GGG - chr2.4:13255500-13255519 None:intergenic 45.0%
GATGAGGATGATGAAATGCC+TGG + chr2.4:13255669-13255688 MS.gene35518:CDS 45.0%
GATGCCCTTAAGGAAGGAAT+GGG + chr2.4:13254316-13254335 MS.gene35518:CDS 45.0%
GTATGGTGAAGATGCCCTTA+AGG + chr2.4:13254306-13254325 MS.gene35518:CDS 45.0%
GTGTGATTGAATTAAGGCCC+GGG + chr2.4:13254132-13254151 MS.gene35518:intron 45.0%
TAACACATACCCCTTGCACT+TGG - chr2.4:13254640-13254659 None:intergenic 45.0%
TAAGGGTGGTGATCCTGAAA+AGG + chr2.4:13253589-13253608 MS.gene35518:CDS 45.0%
TACCAGAGGCCTTTCATGAA+GGG + chr2.4:13255444-13255463 MS.gene35518:CDS 45.0%
TATAGGATCCCCGTAGCTAA+TGG + chr2.4:13253870-13253889 MS.gene35518:intron 45.0%
TCATATACCTCACGCTTCTC+AGG - chr2.4:13254283-13254302 None:intergenic 45.0%
TCCTCCAAAGAGACCTTCAA+GGG - chr2.4:13254564-13254583 None:intergenic 45.0%
TCTGTCTCTTGATCAGGTCA+AGG + chr2.4:13255506-13255525 MS.gene35518:CDS 45.0%
TCTTGATCAGGTCAAGGCTT+TGG + chr2.4:13255512-13255531 MS.gene35518:CDS 45.0%
TGAGTCTCTGTCTCTTGATC+AGG + chr2.4:13255500-13255519 MS.gene35518:CDS 45.0%
TGATCAAGAGACAGAGACTC+AGG - chr2.4:13255501-13255520 None:intergenic 45.0%
TTGAATTAAGGCCCGGGTTA+TGG + chr2.4:13254138-13254157 MS.gene35518:intron 45.0%
! AGTGCAAGGGGTATGTGTTA+TGG + chr2.4:13254640-13254659 MS.gene35518:intron 45.0%
! CAAGGGGTATGTGTTATGGA+AGG + chr2.4:13254644-13254663 MS.gene35518:intron 45.0%
! GAATTAGGGTTGTGGGTTGT+TGG + chr2.4:13253748-13253767 MS.gene35518:intron 45.0%
! GCATCGATCTTTGTCACTGA+TGG - chr2.4:13254881-13254900 None:intergenic 45.0%
! TCAGCAAGAGGCATATGATG+AGG + chr2.4:13255653-13255672 MS.gene35518:CDS 45.0%
!! CCAATTTGCGCTGACTCATT+TGG + chr2.4:13255226-13255245 MS.gene35518:CDS 45.0%
AACTGAGATACAGGCCTTGC+TGG - chr2.4:13255549-13255568 None:intergenic 50.0%
ACTGAGATACAGGCCTTGCT+GGG - chr2.4:13255548-13255567 None:intergenic 50.0%
AGAACCTTGACAACCAGCAC+AGG - chr2.4:13254770-13254789 None:intergenic 50.0%
CACCACCCATTCCTTCCTTA+AGG - chr2.4:13254324-13254343 None:intergenic 50.0%
CAGGCTTGACAACTTCTCCA+GGG - chr2.4:13255280-13255299 None:intergenic 50.0%
CCTCCAAAGAGACCTTCAAG+GGG - chr2.4:13254563-13254582 None:intergenic 50.0%
CTTAAGGAAGGAATGGGTGG+TGG + chr2.4:13254322-13254341 MS.gene35518:CDS 50.0%
GAGGATGATGAAATGCCTGG+TGG + chr2.4:13255672-13255691 MS.gene35518:CDS 50.0%
GGTCAGAAGATTACCTTCCC+TGG + chr2.4:13254976-13254995 MS.gene35518:CDS 50.0%
GGTGAAGATGCCCTTAAGGA+AGG + chr2.4:13254310-13254329 MS.gene35518:CDS 50.0%
GTACCAGAGGCCTTTCATGA+AGG + chr2.4:13255443-13255462 MS.gene35518:CDS 50.0%
GTCTTGTGCTCCAAGTGCAA+GGG + chr2.4:13254627-13254646 MS.gene35518:CDS 50.0%
GTGGTAGTAGCAGAGGAAGA+AGG + chr2.4:13254511-13254530 MS.gene35518:CDS 50.0%
GTTGAGAAGGGGATGCAGAA+TGG + chr2.4:13254955-13254974 MS.gene35518:CDS 50.0%
TCACCCCTTGAAGGTCTCTT+TGG + chr2.4:13254557-13254576 MS.gene35518:CDS 50.0%
TCAGGATCACCACCCTTATC+AGG - chr2.4:13253587-13253606 None:intergenic 50.0%
TCAGTTGACAGACATGGAGC+TGG + chr2.4:13255563-13255582 MS.gene35518:CDS 50.0%
TCATCTTTCTTCGGTGGAGG+TGG + chr2.4:13254376-13254395 MS.gene35518:CDS 50.0%
TCTTGTGCTCCAAGTGCAAG+GGG + chr2.4:13254628-13254647 MS.gene35518:CDS 50.0%
TGGAGACAATTCTCCCAGCA+AGG + chr2.4:13255532-13255551 MS.gene35518:CDS 50.0%
TGTACTCTTTGAGCACCACC+AGG - chr2.4:13255690-13255709 None:intergenic 50.0%
TGTCTTGTGCTCCAAGTGCA+AGG + chr2.4:13254626-13254645 MS.gene35518:CDS 50.0%
TTCATCAGCTTCACCAGGGA+AGG - chr2.4:13254992-13255011 None:intergenic 50.0%
! TCCCTCACTGAGGCTTTATG+TGG + chr2.4:13255200-13255219 MS.gene35518:CDS 50.0%
! TGGAGGTGGATTTCCTGGTA+TGG + chr2.4:13254390-13254409 MS.gene35518:intron 50.0%
!! ATGACCTGTGCTGGTTGTCA+AGG + chr2.4:13254763-13254782 MS.gene35518:CDS 50.0%
!! TGTGCTGGTTGTCAAGGTTC+TGG + chr2.4:13254769-13254788 MS.gene35518:CDS 50.0%
!!! GCTGATGAAGCGGTTGGTTT+TGG + chr2.4:13255003-13255022 MS.gene35518:intron 50.0%
AAGAAGGCAGAGACGTGGTG+AGG + chr2.4:13254527-13254546 MS.gene35518:CDS 55.0%
AAGGCAGCAAGCTCAGCAAG+AGG + chr2.4:13255641-13255660 MS.gene35518:CDS 55.0%
ACCGCTTCATCAGCTTCACC+AGG - chr2.4:13254997-13255016 None:intergenic 55.0%
ACCTTCTCTCCCTTGCACTG+TGG - chr2.4:13254904-13254923 None:intergenic 55.0%
AGAGGAAGAAGGCAGAGACG+TGG + chr2.4:13254522-13254541 MS.gene35518:CDS 55.0%
AGATCGATGCCCACAGTGCA+AGG + chr2.4:13254891-13254910 MS.gene35518:CDS 55.0%
CATGGAGCTGGATGAGTGTG+AGG + chr2.4:13255575-13255594 MS.gene35518:CDS 55.0%
CCAGGCTTGACAACTTCTCC+AGG - chr2.4:13255281-13255300 None:intergenic 55.0%
CCCCTTGAAGGTCTCTTTGG+AGG + chr2.4:13254560-13254579 MS.gene35518:CDS 55.0%
CCCTGGTGAAGCTGATGAAG+CGG + chr2.4:13254993-13255012 MS.gene35518:CDS 55.0%
CCGCTTCATCAGCTTCACCA+GGG - chr2.4:13254996-13255015 None:intergenic 55.0%
CCTGGAGAAGTTGTCAAGCC+TGG + chr2.4:13255278-13255297 MS.gene35518:CDS 55.0%
CCTTCTCTCCCTTGCACTGT+GGG - chr2.4:13254903-13254922 None:intergenic 55.0%
GATCGATGCCCACAGTGCAA+GGG + chr2.4:13254892-13254911 MS.gene35518:CDS 55.0%
GCACACATTGTCCCTCACTG+AGG + chr2.4:13255190-13255209 MS.gene35518:CDS 55.0%
GCCCTTAAGGAAGGAATGGG+TGG + chr2.4:13254319-13254338 MS.gene35518:CDS 55.0%
GGATGTCGTTCACCCCTTGA+AGG + chr2.4:13254548-13254567 MS.gene35518:CDS 55.0%
GGTGAAGCTGATGAAGCGGT+TGG + chr2.4:13254997-13255016 MS.gene35518:intron 55.0%
TGCCCTTCATGAAAGGCCTC+TGG - chr2.4:13255449-13255468 None:intergenic 55.0%
TTGCAGCCGGACACAGTTAC+TGG + chr2.4:13255095-13255114 MS.gene35518:intron 55.0%
! AAGGAAGGAATGGGTGGTGG+CGG + chr2.4:13254325-13254344 MS.gene35518:CDS 55.0%
! ACAGGTGGTGGTAGTAGCAG+AGG + chr2.4:13254504-13254523 MS.gene35518:intron 55.0%
! GAGTGATCCTGAGAAGCGTG+AGG + chr2.4:13254273-13254292 MS.gene35518:CDS 55.0%
! TTCGGTGGAGGTGGATTTCC+TGG + chr2.4:13254385-13254404 MS.gene35518:CDS 55.0%
CCCACAGTGCAAGGGAGAGA+AGG + chr2.4:13254900-13254919 MS.gene35518:CDS 60.0%
GGTGCTTCGATGACCTGTGC+TGG + chr2.4:13254754-13254773 MS.gene35518:CDS 60.0%
!! GAAGGAATGGGTGGTGGCGG+TGG + chr2.4:13254328-13254347 MS.gene35518:CDS 65.0%
!! GGAATGGGTGGTGGCGGTGG+TGG + chr2.4:13254331-13254350 MS.gene35518:CDS 70.0%


Chromosome Type Strat End Strand Name
chr2.4 gene 13253461 13255725 13253461 ID=MS.gene35518
chr2.4 mRNA 13253461 13255725 13253461 ID=MS.gene35518.t1;Parent=MS.gene35518
chr2.4 exon 13253461 13253610 13253461 ID=MS.gene35518.t1.exon1;Parent=MS.gene35518.t1
chr2.4 CDS 13253461 13253610 13253461 ID=cds.MS.gene35518.t1;Parent=MS.gene35518.t1
chr2.4 exon 13254241 13254406 13254241 ID=MS.gene35518.t1.exon2;Parent=MS.gene35518.t1
chr2.4 CDS 13254241 13254406 13254241 ID=cds.MS.gene35518.t1;Parent=MS.gene35518.t1
chr2.4 exon 13254508 13254649 13254508 ID=MS.gene35518.t1.exon3;Parent=MS.gene35518.t1
chr2.4 CDS 13254508 13254649 13254508 ID=cds.MS.gene35518.t1;Parent=MS.gene35518.t1
chr2.4 exon 13254738 13255014 13254738 ID=MS.gene35518.t1.exon4;Parent=MS.gene35518.t1
chr2.4 CDS 13254738 13255014 13254738 ID=cds.MS.gene35518.t1;Parent=MS.gene35518.t1
chr2.4 exon 13255101 13255299 13255101 ID=MS.gene35518.t1.exon5;Parent=MS.gene35518.t1
chr2.4 CDS 13255101 13255299 13255101 ID=cds.MS.gene35518.t1;Parent=MS.gene35518.t1
chr2.4 exon 13255406 13255725 13255406 ID=MS.gene35518.t1.exon6;Parent=MS.gene35518.t1
chr2.4 CDS 13255406 13255725 13255406 ID=cds.MS.gene35518.t1;Parent=MS.gene35518.t1
Gene Sequence

>MS.gene35518

ATGTTTGGAAGAGCGCCTAAGAAGAGTGATAACACTAAATACTATGAGATTCTTGGTGTTTCGAAGAATGCTTCTCCCGATGATTTGAAGAAAGCGTATAAAAAAGCTGCGATTAAGAATCATCCTGATAAGGGTGGTGATCCTGAAAAGGTTGTTTTTTTTTATCTTTGAATTTGATTATTGGATTATCTGATTTGGTTTTTGATTGATCAATATATGTTGATGATTGAATTTGATAATTGTTGTGGTTTGAATAGTGATTAGTTGAATTTAGGGTTAGTTTGATTGAATTAGGGTTGTGGGTTGTTGGTAGAATGATATTTGTTAGGGTTTATTTGTCATGGATTATAAATTTGAGATTTTTAAATTAAGGGGAAGCTATTTTACTTTAAAAAAGTGATTTTTTTACTATAGGATCCCCGTAGCTAATGGGATTGATATTGATGATTACCCAATTGATTGTTGTTAGGTGTATTGGTATTGATTATGCAATTAGGATTGGCGTTAATGCTAAAGATTGATAATTGATTGTTAGTTGTAATGATATTGATGATTGAATTATATAATTGTTGTGGTTTGAATTGTGATTAGATAGTTTTGATTGTTAGTTGTATTGATATTGGGTTATATAATTGTTGTGGTTTGAATTGTGATTAGTTAGTTTTAGGGTAGTGTGATTGAATTAAGGCCCGGGTTATGGTAGTTTGATTTTGGTTTTTGTTTTGATTAGTTAATTGATGAAATTAGGGATTAATTTGTGTTTGTTTGTTTTTGATGTAGTTCAAAGAGCTTGCACAAGCTTATGAGGTTTTGAGTGATCCTGAGAAGCGTGAGGTATATGATCAGTATGGTGAAGATGCCCTTAAGGAAGGAATGGGTGGTGGCGGTGGTGGTCACGATCCATTTGACATCTTTTCATCTTTCTTCGGTGGAGGTGGATTTCCTGGTATGGTTTCTTGTTTGTTATTTTAATTTAGGAGATTAGGGATTATTCAATCGATAACTGTTTGTATGATTGTGTCTTAATCATATTGTATTTGTGAACAGGTGGTGGTAGTAGCAGAGGAAGAAGGCAGAGACGTGGTGAGGATGTCGTTCACCCCTTGAAGGTCTCTTTGGAGGATCTTTACCTTGGGACATCTAAGAAGCTTTCTCTTTCACGAAATGTCTTGTGCTCCAAGTGCAAGGGGTATGTGTTATGGAAGGTCTATCTGCATGTTTGTTGGTATTTTTCTAATGGCTCGGAGTTCTTACATTGTTGATTGGTTTGATTACAGGAAAGGATCAAAGTCTGGTGCTTCGATGACCTGTGCTGGTTGTCAAGGTTCTGGTATGAAGATATCTATGAGGCATTTGGGTGCCAATATGATTCAGCAAATGCAACATCCTTGCAATGAATGTAAGGGTACTGGAGAGACCATCAGTGACAAAGATCGATGCCCACAGTGCAAGGGAGAGAAGGTTGTTCAACAGAAGAAAGTACTTGAAGTTCATGTTGAGAAGGGGATGCAGAATGGTCAGAAGATTACCTTCCCTGGTGAAGCTGATGAAGCGGTTGGTTTTGGATCATACAGTTCCTTAGTTTGAGCTGGATTGTTGAAATTCTAGATACTGATTTTAACTTATTGTTTGTCTTGCAGCCGGACACAGTTACTGGAGATATCGTCTTTGTACTTCAACAGAAGGAACACCCCAAGTTCAAACGAAAGGGTGAAGATCTTTTTGTAGAGCACACATTGTCCCTCACTGAGGCTTTATGTGGTTTCCAATTTGCGCTGACTCATTTGGATTCTCGCCAACTATTGATTAAATCAAACCCTGGAGAAGTTGTCAAGCCTGGTATGTAGATTATTCACATCTGGTTTACTGCATTGTGAAAACATTGTTGAGTGACTGAATTTTTTTCGTCTTTGGTGCTGAAATTTTCAATCTTTTGGTTACGCAGATTCATACAAGGCAATCAATGATGAAGGAATGCCAATGTACCAGAGGCCTTTCATGAAGGGCAAGCTTTACATTCACTTCACAGTGGAGTTCCCTGAGTCTCTGTCTCTTGATCAGGTCAAGGCTTTGGAGACAATTCTCCCAGCAAGGCCTGTATCTCAGTTGACAGACATGGAGCTGGATGAGTGTGAGGAGACAACACTTCATGATGTCAACATTGAAGAAGAGACAAGAAGAAGGCAGCAAGCTCAGCAAGAGGCATATGATGAGGATGATGAAATGCCTGGTGGTGCTCAAAGAGTACAATGTGCTCAGCAGTAA
Protein sequence

>MS.gene35518.t1

MFGRAPKKSDNTKYYEILGVSKNASPDDLKKAYKKAAIKNHPDKGGDPEKFKELAQAYEVLSDPEKREVYDQYGEDALKEGMGGGGGGHDPFDIFSSFFGGGGFPGGGSSRGRRQRRGEDVVHPLKVSLEDLYLGTSKKLSLSRNVLCSKCKGKGSKSGASMTCAGCQGSGMKISMRHLGANMIQQMQHPCNECKGTGETISDKDRCPQCKGEKVVQQKKVLEVHVEKGMQNGQKITFPGEADEAPDTVTGDIVFVLQQKEHPKFKRKGEDLFVEHTLSLTEALCGFQFALTHLDSRQLLIKSNPGEVVKPDSYKAINDEGMPMYQRPFMKGKLYIHFTVEFPESLSLDQVKALETILPARPVSQLTDMELDECEETTLHDVNIEEETRRRQQAQQEAYDEDDEMPGGAQRVQCAQQ