AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene38568


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene38568.t1 MTR_3g099773 98.496 133 2 0 1 133 1 133 8.25e-96 271
MS.gene38568.t1 MTR_5g065550 75.758 132 30 2 2 133 3 132 1.11e-70 208
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene38568.t1 AT4G14600 67.188 128 40 2 7 133 11 137 1.24e-61 186
MS.gene38568.t1 AT1G29060 64.662 133 45 2 2 133 3 134 6.78e-54 166
MS.gene38568.t1 AT1G29060 65.556 90 31 0 44 133 4 93 1.22e-36 120

Find 31 sgRNAs with CRISPR-Local

Find 105 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCCTGGAAAGCCTCACTTTC+TGG 0.084284 3.2:+82841241 None:intergenic
GTTTCACTATTGTGTATCTT+TGG 0.334276 3.2:-82841274 MS.gene38568:CDS
AGGGGCAGCATCGCCGTAAA+AGG 0.359512 3.2:+82843230 None:intergenic
GGAGGACGCAGCAGGAGTTC+TGG 0.386145 3.2:+82843100 None:intergenic
TCAATGTCGAATTCGCTTAG+AGG 0.394928 3.2:-82843258 MS.gene38568:CDS
AAAGGACCACGTGAACAATA+TGG 0.426700 3.2:+82841309 None:intergenic
TTTGTTACCTTGATCGGAAA+GGG 0.442979 3.2:+82843211 None:intergenic
CTGCAGGTTGCTGAAGAGAT+AGG 0.445511 3.2:-82842402 MS.gene38568:intron
ACAGTGGCAAAGGCTCAAGC+TGG 0.467813 3.2:-82841386 MS.gene38568:CDS
AGGGTGTTTGTTACCTTGAT+CGG 0.467925 3.2:+82843205 None:intergenic
GATGAAATTACCGGTCTTCG+TGG 0.475096 3.2:-82843035 MS.gene38568:CDS
GATTTCGATGATGAAATTAC+CGG 0.478157 3.2:-82843044 MS.gene38568:CDS
ATAATCTAAGAAGATTGAAC+AGG 0.480667 3.2:-82841355 MS.gene38568:CDS
AACAGGAGTATCGTGCGAAA+TGG 0.487568 3.2:-82841338 MS.gene38568:CDS
TCGAACTTGACCACGAAGAC+CGG 0.491165 3.2:+82843025 None:intergenic
TCATCATCGAAATCACCAAC+AGG 0.498984 3.2:+82843053 None:intergenic
ATGCTGCCCCTTTCCGATCA+AGG 0.499268 3.2:-82843218 MS.gene38568:intron
AATACGCAATTGAATTTCAT+CGG 0.502875 3.2:+82843079 None:intergenic
ATTTCATCGGAGGACGCAGC+AGG 0.517383 3.2:+82843092 None:intergenic
TGAAACATATTAATGCGAAA+AGG 0.525274 3.2:+82841291 None:intergenic
GTTTGTTACCTTGATCGGAA+AGG 0.528652 3.2:+82843210 None:intergenic
CAGTGGCAAAGGCTCAAGCT+GGG 0.533913 3.2:-82841385 MS.gene38568:CDS
GCAGCAAATGACAGTGGCAA+AGG 0.571535 3.2:-82841396 MS.gene38568:intron
ATGTCGAATTCGCTTAGAGG+TGG 0.594115 3.2:-82843255 MS.gene38568:CDS
CAATTGCGTATTGATCCTGT+TGG 0.644327 3.2:-82843068 MS.gene38568:CDS
TCGAAGATGTCCAGAAAGTG+AGG 0.651544 3.2:-82841251 None:intergenic
AGTGGCAAAGGCTCAAGCTG+GGG 0.653066 3.2:-82841384 MS.gene38568:CDS
TTCAAACCATATTGTTCACG+TGG 0.657520 3.2:-82841315 MS.gene38568:CDS
TTGTTACCTTGATCGGAAAG+GGG 0.660352 3.2:+82843212 None:intergenic
ACGCAATTGAATTTCATCGG+AGG 0.692132 3.2:+82843082 None:intergenic
TTTAATGCAGCAAATGACAG+TGG 0.697287 3.2:-82841402 MS.gene38568:intron

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AATAAAACTTGAAATTATAA+TGG - chr3.2:82842424-82842443 MS.gene38568:intron 10.0%
!! ATAAAACTTGAAATTATAAT+GGG - chr3.2:82842425-82842444 MS.gene38568:intron 10.0%
!!! AAAATATTTCTTTTGTAAAA+TGG + chr3.2:82842515-82842534 None:intergenic 10.0%
!!! TTTAAATTATCATGATTTTT+AGG - chr3.2:82841571-82841590 MS.gene38568:intron 10.0%
!! TAGAGAAAAAAAAAAAAAGA+GGG - chr3.2:82841638-82841657 MS.gene38568:intron 15.0%
!!! AACAAACATTTTTTTAATAC+TGG + chr3.2:82841825-82841844 None:intergenic 15.0%
!!! TTTTTTCTTTTATAAGTCAA+AGG + chr3.2:82842486-82842505 None:intergenic 15.0%
!! AAAAATCTTTGAACCTAATA+TGG + chr3.2:82842031-82842050 None:intergenic 20.0%
!! CTAGAGAAAAAAAAAAAAAG+AGG - chr3.2:82841637-82841656 MS.gene38568:intron 20.0%
!! TGGATTATAACATTAATCTA+TGG - chr3.2:82842859-82842878 MS.gene38568:intron 20.0%
!! TTATCCAATAAATGATGTAT+CGG + chr3.2:82842197-82842216 None:intergenic 20.0%
!!! AAAACAAAGTATTAAGAAGA+CGG + chr3.2:82843079-82843098 None:intergenic 20.0%
!!! ACTTTTTTCATTAGTTTGAT+TGG - chr3.2:82841726-82841745 MS.gene38568:intron 20.0%
!!! GATTTTCTGTTATTTAACTT+GGG + chr3.2:82841803-82841822 None:intergenic 20.0%
!!! TTACTTTAGGATTATTATCT+TGG - chr3.2:82842839-82842858 MS.gene38568:intron 20.0%
! AATACGCAATTGAATTTCAT+CGG + chr3.2:82841432-82841451 None:intergenic 25.0%
! ATAATAATCCTAAAGTAACG+AGG + chr3.2:82842837-82842856 None:intergenic 25.0%
! ATAATCTAAGAAGATTGAAC+AGG - chr3.2:82843153-82843172 MS.gene38568:intron 25.0%
! ATTCATAAACAAGCAAATAC+AGG + chr3.2:82841695-82841714 None:intergenic 25.0%
! CTCAATCAGTAACTTAATTA+GGG - chr3.2:82842377-82842396 MS.gene38568:CDS 25.0%
! GAAGTGAAGTAATTTATGTT+TGG + chr3.2:82842718-82842737 None:intergenic 25.0%
! TAATAGTTTATGATCTCTAG+TGG - chr3.2:82842547-82842566 MS.gene38568:intron 25.0%
! TAGTTTGATGAAACCATATT+AGG - chr3.2:82842015-82842034 MS.gene38568:intron 25.0%
! TCTCAATCAGTAACTTAATT+AGG - chr3.2:82842376-82842395 MS.gene38568:CDS 25.0%
! TGAAACATATTAATGCGAAA+AGG + chr3.2:82843220-82843239 None:intergenic 25.0%
! TTTATAGTGTGTGCATAAAT+GGG + chr3.2:82841538-82841557 None:intergenic 25.0%
!! AAACAAAGTATTAAGAAGAC+GGG + chr3.2:82843078-82843097 None:intergenic 25.0%
!! TTTTATAGTGTGTGCATAAA+TGG + chr3.2:82841539-82841558 None:intergenic 25.0%
!!! GGATTTTCTGTTATTTAACT+TGG + chr3.2:82841804-82841823 None:intergenic 25.0%
!!! TTTTTCATTAGTTTGATTGG+TGG - chr3.2:82841729-82841748 MS.gene38568:intron 25.0%
AAATTCATTCAAATGCAGTG+AGG - chr3.2:82841376-82841395 MS.gene38568:CDS 30.0%
AATTCATTCAAATGCAGTGA+GGG - chr3.2:82841377-82841396 MS.gene38568:CDS 30.0%
AGAGAATCAAAAACGAAAAC+GGG + chr3.2:82842063-82842082 None:intergenic 30.0%
CAGAGAATCAAAAACGAAAA+CGG + chr3.2:82842064-82842083 None:intergenic 30.0%
CTGTATTCTACTCATACATT+GGG - chr3.2:82842218-82842237 MS.gene38568:intron 30.0%
GATTTCGATGATGAAATTAC+CGG - chr3.2:82841464-82841483 MS.gene38568:intron 30.0%
GCTTTATGATATCAGGAAAA+TGG - chr3.2:82842280-82842299 MS.gene38568:intron 30.0%
GTTACATGCTTTATGATATC+AGG - chr3.2:82842273-82842292 MS.gene38568:intron 30.0%
GTTTCACTATTGTGTATCTT+TGG - chr3.2:82843234-82843253 MS.gene38568:CDS 30.0%
TACTAAGACTCTATTACACT+CGG + chr3.2:82841943-82841962 None:intergenic 30.0%
TTATAGTGTGTGCATAAATG+GGG + chr3.2:82841537-82841556 None:intergenic 30.0%
! AATACAGAGGAAACATGTTT+TGG - chr3.2:82842969-82842988 MS.gene38568:intron 30.0%
! ATTGGTTCTTCCTTAACTTT+AGG - chr3.2:82842614-82842633 MS.gene38568:intron 30.0%
! CAAGACTCAAAAAGATTTTC+TGG - chr3.2:82842138-82842157 MS.gene38568:intron 30.0%
! GAAAAGAGTACATGCATATA+TGG - chr3.2:82842251-82842270 MS.gene38568:intron 30.0%
! GTCACTTTCTTATGATGTTT+AGG - chr3.2:82842399-82842418 MS.gene38568:CDS 30.0%
!! TGGATTCAAGTTTTACATAG+AGG + chr3.2:82842574-82842593 None:intergenic 30.0%
AAGAAATTGAGAGAGAGGAA+GGG + chr3.2:82841325-82841344 None:intergenic 35.0%
AATCAAAAACGAAAACGGGA+AGG + chr3.2:82842059-82842078 None:intergenic 35.0%
AATCGAAGAAATTGAGAGAG+AGG + chr3.2:82841330-82841349 None:intergenic 35.0%
ACCTAACCATCCTAAAGTTA+AGG + chr3.2:82842627-82842646 None:intergenic 35.0%
CCAATGTATGAGTAGAATAC+AGG + chr3.2:82842220-82842239 None:intergenic 35.0%
CCTGTATTCTACTCATACAT+TGG - chr3.2:82842217-82842236 MS.gene38568:intron 35.0%
GCTTCAGAAAATGAATACAG+AGG - chr3.2:82842956-82842975 MS.gene38568:intron 35.0%
GTTAAGGAAGAACCAATAGA+TGG + chr3.2:82842611-82842630 None:intergenic 35.0%
TCAGCCGATACATCATTTAT+TGG - chr3.2:82842190-82842209 MS.gene38568:intron 35.0%
TGTTGTCATTGATCTGTCAT+TGG - chr3.2:82843015-82843034 MS.gene38568:CDS 35.0%
TTCAAACCATATTGTTCACG+TGG - chr3.2:82843193-82843212 MS.gene38568:intron 35.0%
TTTAATGCAGCAAATGACAG+TGG - chr3.2:82843106-82843125 MS.gene38568:CDS 35.0%
TTTGTTACCTTGATCGGAAA+GGG + chr3.2:82841300-82841319 None:intergenic 35.0%
! GTTCTTCCTTAACTTTAGGA+TGG - chr3.2:82842618-82842637 MS.gene38568:intron 35.0%
! TAGAAGGCCAAAACTTCTTT+TGG + chr3.2:82842805-82842824 None:intergenic 35.0%
! TATGCATGTACTCTTTTCTC+AGG + chr3.2:82842249-82842268 None:intergenic 35.0%
!! TCCTTAACTTTAGGATGGTT+AGG - chr3.2:82842623-82842642 MS.gene38568:intron 35.0%
!! TGAAGTTCTGAACCATCTAT+TGG - chr3.2:82842596-82842615 MS.gene38568:intron 35.0%
!! TTGATTGGTGGATCGTTTTT+AGG - chr3.2:82841741-82841760 MS.gene38568:intron 35.0%
!!! AAAAGATTTTCTGGAACAGC+TGG - chr3.2:82842147-82842166 MS.gene38568:intron 35.0%
AAAGGACCACGTGAACAATA+TGG + chr3.2:82843202-82843221 None:intergenic 40.0%
AAGACTCTATTACACTCGGT+TGG + chr3.2:82841939-82841958 None:intergenic 40.0%
ACGCAATTGAATTTCATCGG+AGG + chr3.2:82841429-82841448 None:intergenic 40.0%
AGATGGTTCAGAACTTCATC+TGG + chr3.2:82842594-82842613 None:intergenic 40.0%
GAAGAAATTGAGAGAGAGGA+AGG + chr3.2:82841326-82841345 None:intergenic 40.0%
TCATCATCGAAATCACCAAC+AGG + chr3.2:82841458-82841477 None:intergenic 40.0%
TGAGATATGTGTGTTGCAGA+AGG - chr3.2:82841595-82841614 MS.gene38568:intron 40.0%
TTGAGAAACATATCCTGCCA+TGG + chr3.2:82842362-82842381 None:intergenic 40.0%
TTGTTACCTTGATCGGAAAG+GGG + chr3.2:82841299-82841318 None:intergenic 40.0%
TTTCATAACTGATGCTGCCA+CGG - chr3.2:82841904-82841923 MS.gene38568:intron 40.0%
! AGGGTGTTTGTTACCTTGAT+CGG + chr3.2:82841306-82841325 None:intergenic 40.0%
! CAATTGCGTATTGATCCTGT+TGG - chr3.2:82841440-82841459 MS.gene38568:intron 40.0%
! CACTCGTCCAAAAGAAGTTT+TGG - chr3.2:82842795-82842814 MS.gene38568:intron 40.0%
! CTTAGAGGTGGATCCTTTTA+CGG - chr3.2:82841265-82841284 MS.gene38568:CDS 40.0%
! GTTTGTTACCTTGATCGGAA+AGG + chr3.2:82841301-82841320 None:intergenic 40.0%
!! GAACTCTGAAACACTAACCA+TGG - chr3.2:82842342-82842361 MS.gene38568:intron 40.0%
ACTTTGTGCATATCTGCTGC+AGG - chr3.2:82842090-82842109 MS.gene38568:intron 45.0%
ATGTCGAATTCGCTTAGAGG+TGG - chr3.2:82841253-82841272 MS.gene38568:CDS 45.0%
GATGAAATTACCGGTCTTCG+TGG - chr3.2:82841473-82841492 MS.gene38568:intron 45.0%
TAGTGTGTGCATAAATGGGG+AGG + chr3.2:82841534-82841553 None:intergenic 45.0%
! CAGTTGGTCCTCGTTACTTT+AGG - chr3.2:82842826-82842845 MS.gene38568:intron 45.0%
! TCTGAAACACTAACCATGGC+AGG - chr3.2:82842346-82842365 MS.gene38568:intron 45.0%
!! AACAGGAGTATCGTGCGAAA+TGG - chr3.2:82843170-82843189 MS.gene38568:intron 45.0%
!! AGTTTTGGCCTTCTAGCAGT+TGG - chr3.2:82842810-82842829 MS.gene38568:intron 45.0%
!!! CAGAGGAAACATGTTTTGGC+TGG - chr3.2:82842973-82842992 MS.gene38568:intron 45.0%
!! TAATATAAATATATAAACAA+TGG + chr3.2:82842925-82842944 None:intergenic 5.0%
AACGAGGACCAACTGCTAGA+AGG + chr3.2:82842821-82842840 None:intergenic 50.0%
CTGCAGGTTGCTGAAGAGAT+AGG - chr3.2:82842106-82842125 MS.gene38568:intron 50.0%
GCAGCAAATGACAGTGGCAA+AGG - chr3.2:82843112-82843131 MS.gene38568:CDS 50.0%
TCGAACTTGACCACGAAGAC+CGG + chr3.2:82841486-82841505 None:intergenic 50.0%
ACAGTGGCAAAGGCTCAAGC+TGG - chr3.2:82843122-82843141 MS.gene38568:intron 55.0%
AGTGGCAAAGGCTCAAGCTG+GGG - chr3.2:82843124-82843143 MS.gene38568:intron 55.0%
ATGCTGCCCCTTTCCGATCA+AGG - chr3.2:82841290-82841309 MS.gene38568:CDS 55.0%
ATTTCATCGGAGGACGCAGC+AGG + chr3.2:82841419-82841438 None:intergenic 55.0%
CAGTGGCAAAGGCTCAAGCT+GGG - chr3.2:82843123-82843142 MS.gene38568:intron 55.0%
AGGGGCAGCATCGCCGTAAA+AGG + chr3.2:82841281-82841300 None:intergenic 60.0%
TCGGTTGGAAGCAGCATCCG+TGG + chr3.2:82841924-82841943 None:intergenic 60.0%
GGAGGACGCAGCAGGAGTTC+TGG + chr3.2:82841411-82841430 None:intergenic 65.0%


Chromosome Type Strat End Strand Name
chr3.2 gene 82841253 82843277 82841253 ID=MS.gene38568
chr3.2 mRNA 82841253 82843277 82841253 ID=MS.gene38568.t1;Parent=MS.gene38568
chr3.2 exon 82843219 82843277 82843219 ID=MS.gene38568.t1.exon1;Parent=MS.gene38568.t1
chr3.2 CDS 82843219 82843277 82843219 ID=cds.MS.gene38568.t1;Parent=MS.gene38568.t1
chr3.2 exon 82843013 82843136 82843013 ID=MS.gene38568.t1.exon2;Parent=MS.gene38568.t1
chr3.2 CDS 82843013 82843136 82843013 ID=cds.MS.gene38568.t1;Parent=MS.gene38568.t1
chr3.2 exon 82842362 82842418 82842362 ID=MS.gene38568.t1.exon3;Parent=MS.gene38568.t1
chr3.2 CDS 82842362 82842418 82842362 ID=cds.MS.gene38568.t1;Parent=MS.gene38568.t1
chr3.2 exon 82841253 82841414 82841253 ID=MS.gene38568.t1.exon4;Parent=MS.gene38568.t1
chr3.2 CDS 82841253 82841414 82841253 ID=cds.MS.gene38568.t1;Parent=MS.gene38568.t1
Gene Sequence

>MS.gene38568

ATGTCGAATTCGCTTAGAGGTGGATCCTTTTACGGCGATGCTGCCCCTTTCCGATCAAGGTAACAAACACCCTTCCTCTCTCTCAATTTCTTCGATTTAAATTCAATTGATATTGAAATGTTTAAATTCATTCAAATGCAGTGAGGGACTTAGCACCAGAACTCCTGCTGCGTCCTCCGATGAAATTCAATTGCGTATTGATCCTGTTGGTGATTTCGATGATGAAATTACCGGTCTTCGTGGTCAAGTTCGAAAATTGAGAAATGTAATCTTTCTATCCTCCCCATTTATGCACACACTATAAAATTGTTCTTTTTTTTTAAATTATCATGATTTTTAGGTTGAGATATGTGTGTTGCAGAAGGATTATGTTATGTTTTTTATCTAGAGAAAAAAAAAAAAAGAGGGAAAATTAAGAGAATCATATAGTTAAATTGATCCTGTATTTGCTTGTTTATGAATGATGCTTGTTAACTTTTTTCATTAGTTTGATTGGTGGATCGTTTTTAGGTTCACTATAGATAAATCAATACAAGCGACAGAACTACCCAAGTTAAATAACAGAAAATCCAGTATTAAAAAAATGTTTGTTTCTTTTTAGAAAATTTTGTATAGCTGATACGAATGATATGATATTTCATAACTGATATATTTCATAACTGATGCTGCCACGGATGCTGCTTCCAACCGAGTGTAATAGAGTCTTAGTAGCTTGACTTATTGTTGAATTATGTGTTGCTATGTATTTTCTATTGTTATAGTTAGTTTGATGAAACCATATTAGGTTCAAAGATTTTTCTTTTCCTTCCCGTTTTCGTTTTTGATTCTCTGCATATAACTTTGTGCATATCTGCTGCAGGTTGCTGAAGAGATAGGTACAGAAGTCAAGACTCAAAAAGATTTTCTGGAACAGCTGGTACATAATTTTTTTTTACAATCAGCCGATACATCATTTATTGGATAACCTGTATTCTACTCATACATTGGGATTAGCCTGAGAAAAGAGTACATGCATATATGGTTACATGCTTTATGATATCAGGAAAATGGATTTACTAATTACTATCAAAATTTTAATTCTTAGATGTTGAACTCTGAAACACTAACCATGGCAGGATATGTTTCTCAATCAGTAACTTAATTAGGGTCACTTTCTTATGATGTTTAGGATAATAAAACTTGAAATTATAATGGGAATTCGTGCTGTTAATGCTGCTACTTTATTTTGAACCTTTGACTTATAAAAGAAAAAATAAAATCCATTTTACAAAAGAAATATTTTTGTTGTCTACTTTAATAGTTTATGATCTCTAGTGGACCTCTATGTAAAACTTGAATCCAGATGAAGTTCTGAACCATCTATTGGTTCTTCCTTAACTTTAGGATGGTTAGGTTAGAGTTTCGATTTGCATATGAAGATTAATCAAGTTTTACATCAAACTTGCTTTTAGAATATAAACATCCAAACATAAATTACTTCACTTCAATCTCAATTTTAGTTATATGAATTTACAAAATCAAATTTATGCAAAACATTTCAAACACTCGTCCAAAAGAAGTTTTGGCCTTCTAGCAGTTGGTCCTCGTTACTTTAGGATTATTATCTTGGATTATAACATTAATCTATGGTTTTGCATGTGAAAATGAATTAAATTGATGCAAAGTTTGTCCATTGTTTATATATTTATATTAGACGATTTACAGCTTCAGAAAATGAATACAGAGGAAACATGTTTTGGCTGGCTTTTGTTGAGTATTGATGTGTTGTCATTGATCTGTCATTGGTACATCATTCATTACTTTTTTAGAAAGAATATGCATGCCCGTCTTCTTAATACTTTGTTTTTGTCTGTTTTAATGCAGCAAATGACAGTGGCAAAGGCTCAAGCTGGGGTGAAGAATAATCTAAGAAGATTGAACAGGAGTATCGTGCGAAATGGTTCAAACCATATTGTTCACGTGGTCCTTTTCGCATTAATATGTTTCACTATTGTGTATCTTTGGTCGAAGATGTCCAGAAAGTGA

Protein sequence

>MS.gene38568.t1

MSNSLRGGSFYGDAAPFRSSEGLSTRTPAASSDEIQLRIDPVGDFDDEITGLRGQVRKLRNVAEEIGTEVKTQKDFLEQLQMTVAKAQAGVKNNLRRLNRSIVRNGSNHIVHVVLFALICFTIVYLWSKMSRK