AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene38828


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene38828.t1 MTR_7g089080 97.170 106 2 1 1 106 1 105 1.97e-66 216
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 32 sgRNAs with CRISPR-Local

Find 57 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GGAAATGTTGTCACACTAAA+AGG 0.238632 7.1:+17388011 MS.gene38828:CDS
CGTAGACCACTTCTTGATAT+TGG 0.257214 7.1:+17387990 MS.gene38828:CDS
CTCCTTGTGACAGGGCGATT+TGG 0.266023 7.1:-17388047 None:intergenic
CTCTGGGTTGTTGTTGTTGT+TGG 0.304884 7.1:-17387593 None:intergenic
CAGAAGAAGAACGGTGCAGC+AGG 0.408866 7.1:+17387954 MS.gene38828:CDS
GCTCCTCCTGTTGTTGCTGA+TGG 0.409185 7.1:+17388274 MS.gene38828:CDS
AGCAGTTGCTGAAAACAATA+AGG 0.423523 7.1:+17388106 MS.gene38828:CDS
AACTGCTGCTGCTTGTGCAT+TGG 0.426689 7.1:-17388090 None:intergenic
TGTTATAGGTGATGCTGCCT+TGG 0.451586 7.1:+17387917 MS.gene38828:intron
CTGTTGTTTCCCTCCTCCCA+AGG 0.460174 7.1:-17387934 None:intergenic
TGTTGTTGTTGGTGAACAAT+GGG 0.462538 7.1:-17387582 None:intergenic
TTGTTGTTGTTGGTGAACAA+TGG 0.480167 7.1:-17387583 None:intergenic
GGTGATGCTGCCTTGGGAGG+AGG 0.482861 7.1:+17387924 MS.gene38828:intron
GAAGAAGAACGGTGCAGCAG+GGG 0.508581 7.1:+17387956 MS.gene38828:CDS
GCAACTCCATCAGCAACAAC+AGG 0.508824 7.1:-17388280 None:intergenic
ACAACAGGAGGAGCAGGTCC+AGG 0.526077 7.1:-17388265 None:intergenic
GTGATGCTGCCTTGGGAGGA+GGG 0.530719 7.1:+17387925 MS.gene38828:CDS
AAGAACGGTGCAGCAGGGGA+AGG 0.535783 7.1:+17387960 MS.gene38828:CDS
GTTATAGGTGATGCTGCCTT+GGG 0.538221 7.1:+17387918 MS.gene38828:intron
CAATATCAAGAAGTGGTCTA+CGG 0.545242 7.1:-17387989 None:intergenic
TCAGCAACAACAGGAGGAGC+AGG 0.545915 7.1:-17388271 None:intergenic
ACGGTGCAGCAGGGGAAGGA+AGG 0.557061 7.1:+17387964 MS.gene38828:CDS
GCTTGTGTCAATGTGGCACC+TGG 0.568366 7.1:+17388247 MS.gene38828:CDS
AGCCAAATCGCCCTGTCACA+AGG 0.571983 7.1:+17388045 MS.gene38828:CDS
ATAGGTGATGCTGCCTTGGG+AGG 0.594991 7.1:+17387921 MS.gene38828:intron
AGAAGAAGAACGGTGCAGCA+GGG 0.627002 7.1:+17387955 MS.gene38828:CDS
ACATTTCCAATATCAAGAAG+TGG 0.631314 7.1:-17387996 None:intergenic
GGGAAACAACAGAAGAAGAA+CGG 0.643525 7.1:+17387945 MS.gene38828:CDS
ACTCCATCAGCAACAACAGG+AGG 0.651848 7.1:-17388277 None:intergenic
GAAACAAGCTTGTGTCAATG+TGG 0.703443 7.1:+17388240 MS.gene38828:intron
GCAGCAGTGCTAGAAGAGTG+TGG 0.732690 7.1:+17388301 MS.gene38828:CDS
GCAGTGCTAGAAGAGTGTGG+TGG 0.771527 7.1:+17388304 MS.gene38828:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TAAAAAGTTTCGATTTTTTA+GGG + chr7.1:17387802-17387821 MS.gene38828:intron 15.0%
!!! TTAAAAAGTTTCGATTTTTT+AGG + chr7.1:17387801-17387820 MS.gene38828:intron 15.0%
!!! ACTTTTTAAGATTTATCATC+TGG - chr7.1:17387790-17387809 None:intergenic 20.0%
!!! CTTTTTAAGATTTATCATCT+GGG - chr7.1:17387789-17387808 None:intergenic 20.0%
! ACTTTGAGTAAAATAGTTTC+TGG + chr7.1:17387695-17387714 MS.gene38828:intron 25.0%
! CTTTATGGTTTATTTATCCT+GGG + chr7.1:17387646-17387665 MS.gene38828:intron 25.0%
! CTTTGAGTAAAATAGTTTCT+GGG + chr7.1:17387696-17387715 MS.gene38828:intron 25.0%
! TCTTTATGGTTTATTTATCC+TGG + chr7.1:17387645-17387664 MS.gene38828:intron 25.0%
! TTTGCATCAAATCTAGTAAA+GGG + chr7.1:17387826-17387845 MS.gene38828:intron 25.0%
!! TTCTTTTTGGATTTTGTGTT+TGG + chr7.1:17387854-17387873 MS.gene38828:intron 25.0%
!! TTTTGCATCAAATCTAGTAA+AGG + chr7.1:17387825-17387844 MS.gene38828:intron 25.0%
!!! CTTCAATCATTGATTCTTTA+TGG + chr7.1:17387631-17387650 MS.gene38828:intron 25.0%
!!! TGATGATTATTTTCTGAGAT+TGG + chr7.1:17388190-17388209 MS.gene38828:intron 25.0%
ACATTTCCAATATCAAGAAG+TGG - chr7.1:17387999-17388018 None:intergenic 30.0%
ATTGAAAGATCTTGCAAATC+TGG + chr7.1:17387749-17387768 MS.gene38828:intron 30.0%
CACAAACAAACATCAAAAAC+AGG - chr7.1:17387894-17387913 None:intergenic 30.0%
GTTTGTTTGTGCAATGTTAT+AGG + chr7.1:17387903-17387922 MS.gene38828:intron 30.0%
TTGAAAGATCTTGCAAATCT+GGG + chr7.1:17387750-17387769 MS.gene38828:intron 30.0%
AGAAAACACAAACAAACCTC+TGG - chr7.1:17387613-17387632 None:intergenic 35.0%
AGAACAAAAACATTGAACCC+AGG - chr7.1:17387666-17387685 None:intergenic 35.0%
AGCAGTTGCTGAAAACAATA+AGG + chr7.1:17388106-17388125 MS.gene38828:CDS 35.0%
CAATATCAAGAAGTGGTCTA+CGG - chr7.1:17387992-17388011 None:intergenic 35.0%
GAAAACACAAACAAACCTCT+GGG - chr7.1:17387612-17387631 None:intergenic 35.0%
GGAAATGTTGTCACACTAAA+AGG + chr7.1:17388011-17388030 MS.gene38828:CDS 35.0%
! GAGTAAAATAGTTTCTGGGT+TGG + chr7.1:17387700-17387719 MS.gene38828:intron 35.0%
! TGTTGTTGTTGGTGAACAAT+GGG - chr7.1:17387585-17387604 None:intergenic 35.0%
! TTGTTGTTGTTGGTGAACAA+TGG - chr7.1:17387586-17387605 None:intergenic 35.0%
!!! GTAAAGGGTCTTGTTCTTTT+TGG + chr7.1:17387841-17387860 MS.gene38828:intron 35.0%
CACAAAAACTCCTTGTGACA+GGG - chr7.1:17388058-17388077 None:intergenic 40.0%
CGTAGACCACTTCTTGATAT+TGG + chr7.1:17387990-17388009 MS.gene38828:CDS 40.0%
GAAACAAGCTTGTGTCAATG+TGG + chr7.1:17388240-17388259 MS.gene38828:intron 40.0%
GGGAAACAACAGAAGAAGAA+CGG + chr7.1:17387945-17387964 MS.gene38828:CDS 40.0%
CAACAACAACAACAACCCAG+AGG + chr7.1:17387594-17387613 MS.gene38828:CDS 45.0%
GCACAAAAACTCCTTGTGAC+AGG - chr7.1:17388059-17388078 None:intergenic 45.0%
GTTATAGGTGATGCTGCCTT+GGG + chr7.1:17387918-17387937 MS.gene38828:intron 45.0%
TGTTATAGGTGATGCTGCCT+TGG + chr7.1:17387917-17387936 MS.gene38828:intron 45.0%
! CTCTGGGTTGTTGTTGTTGT+TGG - chr7.1:17387596-17387615 None:intergenic 45.0%
ACTCCATCAGCAACAACAGG+AGG - chr7.1:17388280-17388299 None:intergenic 50.0%
AGAAGAAGAACGGTGCAGCA+GGG + chr7.1:17387955-17387974 MS.gene38828:CDS 50.0%
GCAACTCCATCAGCAACAAC+AGG - chr7.1:17388283-17388302 None:intergenic 50.0%
! AACTGCTGCTGCTTGTGCAT+TGG - chr7.1:17388093-17388112 None:intergenic 50.0%
AGCCAAATCGCCCTGTCACA+AGG + chr7.1:17388045-17388064 MS.gene38828:CDS 55.0%
ATAGGTGATGCTGCCTTGGG+AGG + chr7.1:17387921-17387940 MS.gene38828:intron 55.0%
CAGAAGAAGAACGGTGCAGC+AGG + chr7.1:17387954-17387973 MS.gene38828:CDS 55.0%
CTCCTTGTGACAGGGCGATT+TGG - chr7.1:17388050-17388069 None:intergenic 55.0%
GAAGAAGAACGGTGCAGCAG+GGG + chr7.1:17387956-17387975 MS.gene38828:CDS 55.0%
GCAGCAGTGCTAGAAGAGTG+TGG + chr7.1:17388301-17388320 MS.gene38828:CDS 55.0%
GCTCCTCCTGTTGTTGCTGA+TGG + chr7.1:17388274-17388293 MS.gene38828:CDS 55.0%
GCTTGTGTCAATGTGGCACC+TGG + chr7.1:17388247-17388266 MS.gene38828:CDS 55.0%
TCAGCAACAACAGGAGGAGC+AGG - chr7.1:17388274-17388293 None:intergenic 55.0%
! GCAGTGCTAGAAGAGTGTGG+TGG + chr7.1:17388304-17388323 MS.gene38828:CDS 55.0%
!! CTGTTGTTTCCCTCCTCCCA+AGG - chr7.1:17387937-17387956 None:intergenic 55.0%
AAGAACGGTGCAGCAGGGGA+AGG + chr7.1:17387960-17387979 MS.gene38828:CDS 60.0%
ACAACAGGAGGAGCAGGTCC+AGG - chr7.1:17388268-17388287 None:intergenic 60.0%
GTGATGCTGCCTTGGGAGGA+GGG + chr7.1:17387925-17387944 MS.gene38828:CDS 60.0%
ACGGTGCAGCAGGGGAAGGA+AGG + chr7.1:17387964-17387983 MS.gene38828:CDS 65.0%
GGTGATGCTGCCTTGGGAGG+AGG + chr7.1:17387924-17387943 MS.gene38828:intron 65.0%


Chromosome Type Strat End Strand Name
chr7.1 gene 17387570 17388333 17387570 ID=MS.gene38828
chr7.1 mRNA 17387570 17388333 17387570 ID=MS.gene38828.t1;Parent=MS.gene38828
chr7.1 exon 17387570 17387615 17387570 ID=MS.gene38828.t1.exon1;Parent=MS.gene38828.t1
chr7.1 CDS 17387570 17387615 17387570 ID=cds.MS.gene38828.t1;Parent=MS.gene38828.t1
chr7.1 exon 17387925 17388127 17387925 ID=MS.gene38828.t1.exon2;Parent=MS.gene38828.t1
chr7.1 CDS 17387925 17388127 17387925 ID=cds.MS.gene38828.t1;Parent=MS.gene38828.t1
chr7.1 exon 17388241 17388333 17388241 ID=MS.gene38828.t1.exon3;Parent=MS.gene38828.t1
chr7.1 CDS 17388241 17388333 17388241 ID=cds.MS.gene38828.t1;Parent=MS.gene38828.t1
Gene Sequence

>MS.gene38828

ATGGCTTCAAGACCCATTGTTCACCAACAACAACAACAACCCAGAGGTTTGTTTGTGTTTTCTTCAATCATTGATTCTTTATGGTTTATTTATCCTGGGTTCAATGTTTTTGTTCTAAGATTGAAACTTTGAGTAAAATAGTTTCTGGGTTGGTTTCTTTTTCATCTATCTCTTCACTGATTGAAAGATCTTGCAAATCTGGGTTTTTGAAAGAAGCCCAGATGATAAATCTTAAAAAGTTTCGATTTTTTAGGGTTTTGCATCAAATCTAGTAAAGGGTCTTGTTCTTTTTGGATTTTGTGTTTGGATCGAAATCTAGAACCTGTTTTTGATGTTTGTTTGTGCAATGTTATAGGTGATGCTGCCTTGGGAGGAGGGAAACAACAGAAGAAGAACGGTGCAGCAGGGGAAGGAAGGAACCGTAGACCACTTCTTGATATTGGAAATGTTGTCACACTAAAAGGTGTCGAAGTTAAGCCAAATCGCCCTGTCACAAGGAGTTTTTGTGCACAATTACTTGCCAATGCACAAGCAGCAGCAGTTGCTGAAAACAATAAGGTTAGTGATGATTATTTGATTAATCTTTCTTTTCTTTCATTATTTAATTTAATGTTTCATTGTGATGATTATTTTCTGAGATTGGTTTTTTTGTGTTTGATTTGAAATTGAAGAAACAAGCTTGTGTCAATGTGGCACCTGGACCTGCTCCTCCTGTTGTTGCTGATGGAGTTGCAGCAGTGCTAGAAGAGTGTGGTGGCCCCTAA

Protein sequence

>MS.gene38828.t1

MASRPIVHQQQQQPRGDAALGGGKQQKKNGAAGEGRNRRPLLDIGNVVTLKGVEVKPNRPVTRSFCAQLLANAQAAAVAENNKKQACVNVAPGPAPPVVADGVAAVLEECGGP