AlfalfaGEDB | MS.gene43183

M. sativa cultivar XinJiangDaYe / MS.gene43183

KEGG: K03025
GO: GO:0000428 GO:0005575 GO:0005666 GO:0006139 GO:0006351 GO:0006383 GO:0006725 GO:0006807 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009987 GO:0016070 GO:0018130 GO:0019438 GO:0030880 GO:0032774 GO:0032991 GO:0034641 GO:0034645 GO:0034654 GO:0043170 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044271 GO:0046483 GO:0055029 GO:0061695 GO:0071704 GO:0090304 GO:0097659 GO:1901360 GO:1901362 GO:1901576 GO:1902494 GO:1990234
PFAM: PF05158.15

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene43183.t1	XP_024626527.1	97	231	7	0	1	231	1	231	2.50E-123	451.4

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene43183.t1	Q921X6	25.5	243	152	5	12	231	80	316	1.4e-18	94.7

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene43183.t1	A0A396GUA7	97.0	231	7	0	1	231	1	231	1.8e-123	451.4

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene049142	MS.gene43183	0.802684	4.99E-49	-1.69E-46
MS.gene049159	MS.gene43183	0.820813	5.63E-53	-1.69E-46
MS.gene049368	MS.gene43183	0.801106	1.05E-48	-1.69E-46
MS.gene049404	MS.gene43183	0.815481	9.04E-52	-1.69E-46
MS.gene050237	MS.gene43183	0.805462	1.32E-49	-1.69E-46
MS.gene051247	MS.gene43183	0.816666	4.92E-52	-1.69E-46
MS.gene051719	MS.gene43183	0.801331	9.47E-49	-1.69E-46
MS.gene052516	MS.gene43183	0.817698	2.88E-52	-1.69E-46
MS.gene052519	MS.gene43183	0.81513	1.08E-51	-1.69E-46
MS.gene052773	MS.gene43183	0.809502	1.83E-50	-1.69E-46
MS.gene053006	MS.gene43183	0.811099	8.29E-51	-1.69E-46
MS.gene053008	MS.gene43183	0.839882	1.21E-57	-1.69E-46
MS.gene053216	MS.gene43183	0.804335	2.27E-49	-1.69E-46
MS.gene053226	MS.gene43183	0.818896	1.54E-52	-1.69E-46
MS.gene054651	MS.gene43183	0.806899	6.58E-50	-1.69E-46
MS.gene055818	MS.gene43183	0.814938	1.19E-51	-1.69E-46
MS.gene055841	MS.gene43183	0.80098	1.12E-48	-1.69E-46
MS.gene055926	MS.gene43183	0.806143	9.49E-50	-1.69E-46
MS.gene056173	MS.gene43183	0.81111	8.25E-51	-1.69E-46
MS.gene056174	MS.gene43183	0.809719	1.65E-50	-1.69E-46
MS.gene056366	MS.gene43183	0.816475	5.42E-52	-1.69E-46
MS.gene05690	MS.gene43183	0.816005	6.91E-52	-1.69E-46
MS.gene057208	MS.gene43183	0.818535	1.86E-52	-1.69E-46
MS.gene057287	MS.gene43183	0.808839	2.54E-50	-1.69E-46
MS.gene057598	MS.gene43183	0.80904	2.30E-50	-1.69E-46
MS.gene057693	MS.gene43183	0.822107	2.83E-53	-1.69E-46
MS.gene057855	MS.gene43183	0.804648	1.95E-49	-1.69E-46
MS.gene05795	MS.gene43183	0.827807	1.28E-54	-1.69E-46
MS.gene058008	MS.gene43183	0.804303	2.30E-49	-1.69E-46
MS.gene058009	MS.gene43183	0.835699	1.44E-56	-1.69E-46
MS.gene058595	MS.gene43183	0.802835	4.65E-49	-1.69E-46
MS.gene058625	MS.gene43183	0.807799	4.24E-50	-1.69E-46
MS.gene058783	MS.gene43183	0.800352	1.50E-48	-1.69E-46
MS.gene058979	MS.gene43183	0.828966	6.70E-55	-1.69E-46
MS.gene05954	MS.gene43183	0.801833	7.47E-49	-1.69E-46
MS.gene059842	MS.gene43183	0.812226	4.72E-51	-1.69E-46
MS.gene060322	MS.gene43183	0.829653	4.57E-55	-1.69E-46
MS.gene060907	MS.gene43183	0.809284	2.04E-50	-1.69E-46

PPI:

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene43183.t1	MTR_0003s0690	94.220	173	10	0	59	231	1	173	6.65e-122	343
MS.gene43183.t1	MTR_7g109780	73.057	193	47	2	44	231	1	193	8.46e-102	293
MS.gene43183.t1	MTR_7g109760	42.771	166	38	5	1	164	1	111	8.85e-32	112

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene43183.t1	AT5G23710	37.209	215	128	3	21	231	19	230	2.49e-47	156

Find 37 sgRNAs with CRISPR-Local

Find 56 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
CAGATGACAAGTACTGGATA+TGG	0.326445	7.2:+78473108	MS.gene43183:CDS
ATGAAACGTTTGCAAGAATC+TGG	0.362818	7.2:+78472616	MS.gene43183:CDS
GATGTTTGGAGTGGGTTAAA+AGG	0.362955	7.2:+78473004	MS.gene43183:CDS
GGCACTTATAAACAAGAGTA+TGG	0.381632	7.2:+78472795	MS.gene43183:CDS
GAGCCTTCCAAGGATATAAC+TGG	0.383383	7.2:+78472877	MS.gene43183:CDS
TGAAAATAGACACATGTTGT+TGG	0.391859	7.2:-78473270	None:intergenic
TTCCACATGTGATGGATGTT+TGG	0.402495	7.2:+78472990	MS.gene43183:CDS
GTGCTTGCAATGCATTTCAT+TGG	0.426796	7.2:+78472960	MS.gene43183:CDS
TAGAGTATACGCTCTTCATC+TGG	0.446970	7.2:-78472676	None:intergenic
TGTTTATTAAGCGCAGCAAT+AGG	0.447212	7.2:-78472766	None:intergenic
ATGCCCACCAGTTATATCCT+TGG	0.459091	7.2:-78472884	None:intergenic
TGGAGTGGGTTAAAAGGAGT+GGG	0.480413	7.2:+78473010	MS.gene43183:CDS
AGATGACAAGTACTGGATAT+GGG	0.485810	7.2:+78473109	MS.gene43183:CDS
CAAAGGTATAAAGATCTACA+TGG	0.502338	7.2:+78472843	MS.gene43183:CDS
ATAATGCAGATGACAAGTAC+TGG	0.506988	7.2:+78473102	MS.gene43183:CDS
ATACTTAGCAAGGAAAACTC+AGG	0.536886	7.2:+78472706	MS.gene43183:CDS
TATAAACAAGAGTATGGTAA+AGG	0.546896	7.2:+78472801	MS.gene43183:CDS
TTGGAGTGGGTTAAAAGGAG+TGG	0.562912	7.2:+78473009	MS.gene43183:CDS
AACAGAATTTGAGCCTTCCA+AGG	0.568058	7.2:+78472867	MS.gene43183:CDS
TATTAAGCGCAGCAATAGGA+AGG	0.568531	7.2:-78472762	None:intergenic
GAGAGAGTTTCCACATGTGA+TGG	0.576485	7.2:+78472982	MS.gene43183:CDS
TTGTTACATATGCAAAAGCA+AGG	0.588438	7.2:+78473161	MS.gene43183:CDS
GTCATCACAACTCAAGTCAC+GGG	0.589333	7.2:+78473033	MS.gene43183:CDS
TGCGCTTAATAAACACATGA+AGG	0.598707	7.2:+78472774	MS.gene43183:CDS
CTATGACCTTATACTTAGCA+AGG	0.601401	7.2:+78472696	MS.gene43183:CDS
CCACCAGTTATATCCTTGGA+AGG	0.602371	7.2:-78472880	None:intergenic
GGTCATCACAACTCAAGTCA+CGG	0.608686	7.2:+78473032	MS.gene43183:CDS
CCTTCCAAGGATATAACTGG+TGG	0.618508	7.2:+78472880	MS.gene43183:CDS
CTCCAAACATCCATCACATG+TGG	0.618959	7.2:-78472992	None:intergenic
TACTTAGCAAGGAAAACTCA+GGG	0.624253	7.2:+78472707	MS.gene43183:CDS
GATGACAAGTACTGGATATG+GGG	0.631855	7.2:+78473110	MS.gene43183:CDS
GGAGTGGGTTAAAAGGAGTG+GGG	0.639610	7.2:+78473011	MS.gene43183:CDS
CATGTGATGGATGTTTGGAG+TGG	0.639686	7.2:+78472995	MS.gene43183:CDS
ATGTGATGGATGTTTGGAGT+GGG	0.644389	7.2:+78472996	MS.gene43183:CDS
TGTTACATATGCAAAAGCAA+GGG	0.651007	7.2:+78473162	MS.gene43183:CDS
AAAGGTATAAAGATCTACAT+GGG	0.652631	7.2:+78472844	MS.gene43183:CDS
CTTCCAAGGATATAACTGGT+GGG	0.673279	7.2:+78472881	MS.gene43183:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!!	CTTTATACCTTTGTTTTTAA+TGG	-	chr7.2:78472836-78472855	None:intergenic	20.0%
!!	AAAGGTATAAAGATCTACAT+GGG	+	chr7.2:78472844-78472863	MS.gene43183:CDS	25.0%
!!	TATAAACAAGAGTATGGTAA+AGG	+	chr7.2:78472801-78472820	MS.gene43183:CDS	25.0%
!!!	ATACCTTTGTTTTTAATGGA+AGG	-	chr7.2:78472832-78472851	None:intergenic	25.0%
	CTTCCTTCCATTAAAAACAA+AGG	+	chr7.2:78472826-78472845	MS.gene43183:CDS	30.0%
	TGAAAATAGACACATGTTGT+TGG	-	chr7.2:78473273-78473292	None:intergenic	30.0%
	TGACAAGAAAAACATGGAAA+GGG	-	chr7.2:78473213-78473232	None:intergenic	30.0%
	TGTTACATATGCAAAAGCAA+GGG	+	chr7.2:78473162-78473181	MS.gene43183:CDS	30.0%
	TTGACAAGAAAAACATGGAA+AGG	-	chr7.2:78473214-78473233	None:intergenic	30.0%
	TTGTTACATATGCAAAAGCA+AGG	+	chr7.2:78473161-78473180	MS.gene43183:CDS	30.0%
!	AGAAGAGATTTTGCAAACTT+TGG	+	chr7.2:78473065-78473084	MS.gene43183:CDS	30.0%
!	AGTTTTCCTTGCTAAGTATA+AGG	-	chr7.2:78472705-78472724	None:intergenic	30.0%
!	CAAAGGTATAAAGATCTACA+TGG	+	chr7.2:78472843-78472862	MS.gene43183:CDS	30.0%
!	CATGTGTCTATTTTCAGAAA+TGG	+	chr7.2:78473277-78473296	MS.gene43183:CDS	30.0%
!	TGTCTATTTTCAGAAATGGT+TGG	+	chr7.2:78473281-78473300	MS.gene43183:CDS	30.0%
!	TTCCATGTTTTTCTTGTCAA+CGG	+	chr7.2:78473214-78473233	MS.gene43183:CDS	30.0%
	ACAGAAACGACAAAAACTGT+CGG	+	chr7.2:78472648-78472667	MS.gene43183:CDS	35.0%
	ATAATGCAGATGACAAGTAC+TGG	+	chr7.2:78473102-78473121	MS.gene43183:CDS	35.0%
	ATACTTAGCAAGGAAAACTC+AGG	+	chr7.2:78472706-78472725	MS.gene43183:CDS	35.0%
	ATCCGTTGACAAGAAAAACA+TGG	-	chr7.2:78473219-78473238	None:intergenic	35.0%
	ATGAAACGTTTGCAAGAATC+TGG	+	chr7.2:78472616-78472635	MS.gene43183:CDS	35.0%
	CTATGACCTTATACTTAGCA+AGG	+	chr7.2:78472696-78472715	MS.gene43183:CDS	35.0%
	GGCACTTATAAACAAGAGTA+TGG	+	chr7.2:78472795-78472814	MS.gene43183:CDS	35.0%
	TACTTAGCAAGGAAAACTCA+GGG	+	chr7.2:78472707-78472726	MS.gene43183:CDS	35.0%
	TGCGCTTAATAAACACATGA+AGG	+	chr7.2:78472774-78472793	MS.gene43183:CDS	35.0%
	TGTTTATTAAGCGCAGCAAT+AGG	-	chr7.2:78472769-78472788	None:intergenic	35.0%
!	ATGTAACAAGTTTTGCCAAC+AGG	-	chr7.2:78473150-78473169	None:intergenic	35.0%
!	GATTTTGCATCTATTCCTGT+TGG	+	chr7.2:78473132-78473151	MS.gene43183:CDS	35.0%
!!	AGATGACAAGTACTGGATAT+GGG	+	chr7.2:78473109-78473128	MS.gene43183:CDS	35.0%
!!	TTTTTGTCGTTTCTGTTGAG+AGG	-	chr7.2:78472644-78472663	None:intergenic	35.0%
	AACAGAATTTGAGCCTTCCA+AGG	+	chr7.2:78472867-78472886	MS.gene43183:CDS	40.0%
	CTTCCAAGGATATAACTGGT+GGG	+	chr7.2:78472881-78472900	MS.gene43183:CDS	40.0%
	GTGCTTGCAATGCATTTCAT+TGG	+	chr7.2:78472960-78472979	MS.gene43183:CDS	40.0%
	TAGAGTATACGCTCTTCATC+TGG	-	chr7.2:78472679-78472698	None:intergenic	40.0%
	TATTAAGCGCAGCAATAGGA+AGG	-	chr7.2:78472765-78472784	None:intergenic	40.0%
!	ACGGATGAGCTTTTGTTCAA+CGG	+	chr7.2:78473233-78473252	MS.gene43183:CDS	40.0%
!	ATGAGCTTTTGTTCAACGGA+TGG	+	chr7.2:78473237-78473256	MS.gene43183:CDS	40.0%
!!	ATGTGATGGATGTTTGGAGT+GGG	+	chr7.2:78472996-78473015	MS.gene43183:CDS	40.0%
!!	CAGATGACAAGTACTGGATA+TGG	+	chr7.2:78473108-78473127	MS.gene43183:CDS	40.0%
!!	GATGACAAGTACTGGATATG+GGG	+	chr7.2:78473110-78473129	MS.gene43183:CDS	40.0%
!!	GATGTTTGGAGTGGGTTAAA+AGG	+	chr7.2:78473004-78473023	MS.gene43183:CDS	40.0%
!!	TTCCACATGTGATGGATGTT+TGG	+	chr7.2:78472990-78473009	MS.gene43183:CDS	40.0%
	ATGCCCACCAGTTATATCCT+TGG	-	chr7.2:78472887-78472906	None:intergenic	45.0%
	CCACCAGTTATATCCTTGGA+AGG	-	chr7.2:78472883-78472902	None:intergenic	45.0%
	CCTTCCAAGGATATAACTGG+TGG	+	chr7.2:78472880-78472899	MS.gene43183:CDS	45.0%
	CTCCAAACATCCATCACATG+TGG	-	chr7.2:78472995-78473014	None:intergenic	45.0%
	GAGAGAGTTTCCACATGTGA+TGG	+	chr7.2:78472982-78473001	MS.gene43183:CDS	45.0%
	GAGCCTTCCAAGGATATAAC+TGG	+	chr7.2:78472877-78472896	MS.gene43183:CDS	45.0%
	GGTCATCACAACTCAAGTCA+CGG	+	chr7.2:78473032-78473051	MS.gene43183:CDS	45.0%
	GTCATCACAACTCAAGTCAC+GGG	+	chr7.2:78473033-78473052	MS.gene43183:CDS	45.0%
!	GTGGGCATTTTTACAGCGAA+GGG	+	chr7.2:78472899-78472918	MS.gene43183:CDS	45.0%
!!	CATGTGATGGATGTTTGGAG+TGG	+	chr7.2:78472995-78473014	MS.gene43183:CDS	45.0%
!!	TGGAGTGGGTTAAAAGGAGT+GGG	+	chr7.2:78473010-78473029	MS.gene43183:CDS	45.0%
!!	TTGGAGTGGGTTAAAAGGAG+TGG	+	chr7.2:78473009-78473028	MS.gene43183:CDS	45.0%
!	GGTGGGCATTTTTACAGCGA+AGG	+	chr7.2:78472898-78472917	MS.gene43183:CDS	50.0%
!!	GGAGTGGGTTAAAAGGAGTG+GGG	+	chr7.2:78473011-78473030	MS.gene43183:CDS	50.0%

Chromosome	Type	Strat	End	Strand	Name
chr7.2	gene	78472616	78473311	78472616	ID=MS.gene43183
chr7.2	mRNA	78472616	78473311	78472616	ID=MS.gene43183.t1;Parent=MS.gene43183
chr7.2	exon	78472616	78473311	78472616	ID=MS.gene43183.t1.exon1;Parent=MS.gene43183.t1
chr7.2	CDS	78472616	78473311	78472616	ID=cds.MS.gene43183.t1;Parent=MS.gene43183.t1

Gene Sequence

>MS.gene43183

ATGAAACGTTTGCAAGAATCTGGTTCCTCTCAACAGAAACGACAAAAACTGTCGGCGCTGCCAGATGAAGAGCGTATACTCTATGACCTTATACTTAGCAAGGAAAACTCAGGGATCTCAAAAACAGACATGAGAAGAGAAACAAACCTTCCTATTGCTGCGCTTAATAAACACATGAAGGCACTTATAAACAAGAGTATGGTAAAGGAACTTCCTTCCATTAAAAACAAAGGTATAAAGATCTACATGGGAACAGAATTTGAGCCTTCCAAGGATATAACTGGTGGGCATTTTTACAGCGAAGGGAGTCTTGATACGCTTTTTATAGACGCTCTAAAGACAGTGTGCTTGCAATGCATTTCATTGGAGAGAGTTTCCACATGTGATGGATGTTTGGAGTGGGTTAAAAGGAGTGGGGTCATCACAACTCAAGTCACGGGAGAACAAATAGAAGAGATTTTGCAAACTTTGGTTTTAGATGATGAGATAATGCAGATGACAAGTACTGGATATGGGGATTTTGCATCTATTCCTGTTGGCAAAACTTGTTACATATGCAAAAGCAAGGGTGAAAAGAAATCTGCTGACTCGACTCCCTTTCCATGTTTTTCTTGTCAACGGATGAGCTTTTGTTCAACGGATGGCGTTGTCTCTCCAACAACATGTGTCTATTTTCAGAAATGGTTGGATTTCTGA

Protein sequence

>MS.gene43183.t1

MKRLQESGSSQQKRQKLSALPDEERILYDLILSKENSGISKTDMRRETNLPIAALNKHMKALINKSMVKELPSIKNKGIKIYMGTEFEPSKDITGGHFYSEGSLDTLFIDALKTVCLQCISLERVSTCDGCLEWVKRSGVITTQVTGEQIEEILQTLVLDDEIMQMTSTGYGDFASIPVGKTCYICKSKGEKKSADSTPFPCFSCQRMSFCSTDGVVSPTTCVYFQKWLDF