Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene44631.t1 | RHN44405.1 | 86.7 | 98 | 13 | 0 | 1 | 98 | 336 | 433 | 5.50E-40 | 173.3 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene44631.t1 | Q9LRQ8 | 55.9 | 68 | 30 | 0 | 29 | 96 | 383 | 450 | 8.9e-15 | 80.9 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene44631.t1 | B4Y0U1 | 86.7 | 98 | 13 | 0 | 1 | 98 | 386 | 483 | 3.9e-40 | 173.3 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049887 | MS.gene44631 | 0.84491 | 5.64E-59 | -1.69E-46 |
| MS.gene049983 | MS.gene44631 | 0.806298 | 8.80E-50 | -1.69E-46 |
| MS.gene050386 | MS.gene44631 | 0.800861 | 1.18E-48 | -1.69E-46 |
| MS.gene050654 | MS.gene44631 | 0.81085 | 9.39E-51 | -1.69E-46 |
| MS.gene050838 | MS.gene44631 | 0.858577 | 7.49E-63 | -1.69E-46 |
| MS.gene050941 | MS.gene44631 | 0.807288 | 5.44E-50 | -1.69E-46 |
| MS.gene051003 | MS.gene44631 | 0.826339 | 2.86E-54 | -1.69E-46 |
| MS.gene051234 | MS.gene44631 | 0.817878 | 2.62E-52 | -1.69E-46 |
| MS.gene051328 | MS.gene44631 | 0.815564 | 8.67E-52 | -1.69E-46 |
| MS.gene051577 | MS.gene44631 | 0.809776 | 1.60E-50 | -1.69E-46 |
| MS.gene051852 | MS.gene44631 | 0.827892 | 1.22E-54 | -1.69E-46 |
| MS.gene052133 | MS.gene44631 | 0.806955 | 6.40E-50 | -1.69E-46 |
| MS.gene052335 | MS.gene44631 | 0.813443 | 2.55E-51 | -1.69E-46 |
| MS.gene052342 | MS.gene44631 | 0.835584 | 1.54E-56 | -1.69E-46 |
| MS.gene052397 | MS.gene44631 | 0.802826 | 4.67E-49 | -1.69E-46 |
| MS.gene052459 | MS.gene44631 | 0.824433 | 8.09E-54 | -1.69E-46 |
| MS.gene052463 | MS.gene44631 | 0.814044 | 1.88E-51 | -1.69E-46 |
| MS.gene052516 | MS.gene44631 | 0.832065 | 1.17E-55 | -1.69E-46 |
| MS.gene053058 | MS.gene44631 | 0.801003 | 1.11E-48 | -1.69E-46 |
| MS.gene053581 | MS.gene44631 | 0.820769 | 5.76E-53 | -1.69E-46 |
| MS.gene053786 | MS.gene44631 | 0.813525 | 2.45E-51 | -1.69E-46 |
| MS.gene055465 | MS.gene44631 | 0.830315 | 3.15E-55 | -1.69E-46 |
| MS.gene055466 | MS.gene44631 | 0.82115 | 4.70E-53 | -1.69E-46 |
| MS.gene055468 | MS.gene44631 | 0.847395 | 1.19E-59 | -1.69E-46 |
| MS.gene055469 | MS.gene44631 | 0.826716 | 2.33E-54 | -1.69E-46 |
| MS.gene055513 | MS.gene44631 | 0.800092 | 1.70E-48 | -1.69E-46 |
| MS.gene055580 | MS.gene44631 | -0.801619 | 8.27E-49 | -1.69E-46 |
| MS.gene055979 | MS.gene44631 | 0.825634 | 4.21E-54 | -1.69E-46 |
| MS.gene056043 | MS.gene44631 | -0.818237 | 2.18E-52 | -1.69E-46 |
| MS.gene056181 | MS.gene44631 | 0.810113 | 1.35E-50 | -1.69E-46 |
| MS.gene056390 | MS.gene44631 | 0.808204 | 3.47E-50 | -1.69E-46 |
| MS.gene056801 | MS.gene44631 | 0.803404 | 3.54E-49 | -1.69E-46 |
| MS.gene057287 | MS.gene44631 | 0.802448 | 5.58E-49 | -1.69E-46 |
| MS.gene058261 | MS.gene44631 | 0.816244 | 6.11E-52 | -1.69E-46 |
| MS.gene058720 | MS.gene44631 | 0.802325 | 5.92E-49 | -1.69E-46 |
| MS.gene059170 | MS.gene44631 | 0.840673 | 7.55E-58 | -1.69E-46 |
| MS.gene060563 | MS.gene44631 | 0.802524 | 5.38E-49 | -1.69E-46 |
| MS.gene060932 | MS.gene44631 | 0.821733 | 3.45E-53 | -1.69E-46 |
| MS.gene061006 | MS.gene44631 | 0.822974 | 1.78E-53 | -1.69E-46 |
| MS.gene061100 | MS.gene44631 | 0.836762 | 7.74E-57 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene44631.t1 | MTR_7g013850 | 86.735 | 98 | 13 | 0 | 1 | 98 | 386 | 483 | 2.20e-56 | 181 |
| MS.gene44631.t1 | MTR_7g014670 | 62.887 | 97 | 36 | 0 | 2 | 98 | 166 | 262 | 6.36e-35 | 120 |
| MS.gene44631.t1 | MTR_7g014160 | 60.638 | 94 | 37 | 0 | 2 | 95 | 380 | 473 | 8.70e-35 | 124 |
| MS.gene44631.t1 | MTR_7g014200 | 61.053 | 95 | 36 | 1 | 2 | 95 | 374 | 468 | 2.08e-33 | 120 |
| MS.gene44631.t1 | MTR_7g014610 | 62.105 | 95 | 35 | 1 | 2 | 95 | 381 | 475 | 7.59e-32 | 115 |
| MS.gene44631.t1 | MTR_7g014620 | 56.842 | 95 | 40 | 1 | 2 | 95 | 338 | 432 | 5.64e-23 | 91.3 |
| MS.gene44631.t1 | MTR_6g015315 | 41.053 | 95 | 56 | 0 | 1 | 95 | 358 | 452 | 1.95e-20 | 84.3 |
| MS.gene44631.t1 | MTR_6g015850 | 41.667 | 96 | 50 | 2 | 2 | 95 | 366 | 457 | 3.02e-19 | 81.3 |
| MS.gene44631.t1 | MTR_6g015830 | 43.750 | 96 | 48 | 2 | 2 | 95 | 369 | 460 | 1.69e-18 | 79.0 |
| MS.gene44631.t1 | MTR_6g015815 | 47.761 | 67 | 35 | 0 | 29 | 95 | 394 | 460 | 1.28e-16 | 73.6 |
| MS.gene44631.t1 | MTR_6g015320 | 36.735 | 98 | 59 | 1 | 1 | 95 | 357 | 454 | 2.77e-16 | 72.8 |
| MS.gene44631.t1 | MTR_2g089765 | 43.333 | 90 | 51 | 0 | 6 | 95 | 393 | 482 | 5.86e-16 | 71.6 |
| MS.gene44631.t1 | MTR_3g028210 | 44.928 | 69 | 38 | 0 | 27 | 95 | 396 | 464 | 3.14e-15 | 69.7 |
| MS.gene44631.t1 | MTR_7g014230 | 40.000 | 95 | 55 | 2 | 4 | 97 | 367 | 460 | 8.71e-15 | 68.6 |
| MS.gene44631.t1 | MTR_7g014330 | 41.489 | 94 | 51 | 3 | 4 | 95 | 365 | 456 | 6.49e-14 | 65.9 |
| MS.gene44631.t1 | MTR_7g014360 | 40.206 | 97 | 54 | 3 | 1 | 95 | 292 | 386 | 4.49e-13 | 63.5 |
| MS.gene44631.t1 | MTR_7g014250 | 37.500 | 96 | 58 | 2 | 1 | 95 | 362 | 456 | 5.54e-13 | 63.2 |
| MS.gene44631.t1 | MTR_3g087640 | 42.308 | 78 | 42 | 2 | 18 | 95 | 398 | 472 | 7.53e-13 | 62.8 |
| MS.gene44631.t1 | MTR_2g089670 | 42.222 | 90 | 52 | 0 | 6 | 95 | 388 | 477 | 8.56e-13 | 62.8 |
| MS.gene44631.t1 | MTR_3g435290 | 41.791 | 67 | 37 | 1 | 29 | 95 | 53 | 117 | 3.53e-12 | 58.2 |
| MS.gene44631.t1 | MTR_6g015310 | 39.726 | 73 | 44 | 0 | 1 | 73 | 261 | 333 | 5.07e-12 | 60.5 |
| MS.gene44631.t1 | MTR_2g089745 | 41.791 | 67 | 39 | 0 | 29 | 95 | 11 | 77 | 2.34e-11 | 55.1 |
| MS.gene44631.t1 | MTR_2g089650 | 44.776 | 67 | 35 | 1 | 29 | 95 | 399 | 463 | 2.66e-11 | 58.5 |
| MS.gene44631.t1 | MTR_2g089735 | 39.474 | 76 | 44 | 1 | 20 | 95 | 388 | 461 | 5.79e-11 | 57.8 |
| MS.gene44631.t1 | MTR_2g089755 | 41.791 | 67 | 37 | 1 | 29 | 95 | 411 | 475 | 5.98e-11 | 57.4 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene44631.t1 | AT5G39050 | 49.367 | 79 | 40 | 0 | 17 | 95 | 389 | 467 | 6.60e-20 | 83.2 |
| MS.gene44631.t1 | AT3G29670 | 55.882 | 68 | 30 | 0 | 29 | 96 | 383 | 450 | 1.13e-19 | 82.4 |
| MS.gene44631.t1 | AT3G29590 | 52.055 | 73 | 35 | 0 | 23 | 95 | 376 | 448 | 4.13e-18 | 77.8 |
| MS.gene44631.t1 | AT1G03940 | 46.269 | 67 | 36 | 0 | 29 | 95 | 398 | 464 | 1.33e-17 | 76.6 |
| MS.gene44631.t1 | AT3G29635 | 40.860 | 93 | 52 | 1 | 6 | 95 | 362 | 454 | 3.11e-16 | 72.8 |
| MS.gene44631.t1 | AT5G39090 | 45.070 | 71 | 39 | 0 | 25 | 95 | 377 | 447 | 5.40e-15 | 68.9 |
| MS.gene44631.t1 | AT5G39080 | 54.098 | 61 | 28 | 0 | 25 | 85 | 391 | 451 | 7.97e-15 | 68.6 |
| MS.gene44631.t1 | AT5G61160 | 42.029 | 69 | 40 | 0 | 23 | 91 | 378 | 446 | 1.07e-11 | 59.7 |
Find 20 sgRNAs with CRISPR-Local
Find 25 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TTGAAAGGTGGTGTTGAATT+TGG | 0.210135 | 7.3:-83656328 | MS.gene44631:CDS |
| GGAGTATATGAAATTGATTT+TGG | 0.266147 | 7.3:-83656427 | MS.gene44631:CDS |
| TCTAAATATTTGACTATGAT+TGG | 0.335587 | 7.3:-83656493 | MS.gene44631:CDS |
| ATTGATAGAGGATTAACTAT+TGG | 0.368461 | 7.3:-83656370 | MS.gene44631:CDS |
| TATTGGTTTAACAGAAAGTA+AGG | 0.387767 | 7.3:-83656353 | MS.gene44631:CDS |
| ATGGATAAGGGTGTTCTTGA+TGG | 0.402404 | 7.3:-83656532 | MS.gene44631:CDS |
| ATTCAAGGAATAGGAGTTGC+AGG | 0.408252 | 7.3:-83656463 | MS.gene44631:CDS |
| GGTGAAGGAATTCAAGGAAT+AGG | 0.425888 | 7.3:-83656472 | MS.gene44631:CDS |
| GAGATTACTTCAATTGATAG+AGG | 0.456941 | 7.3:-83656382 | MS.gene44631:CDS |
| ATGATTGGTGAAGGAATTCA+AGG | 0.487904 | 7.3:-83656478 | MS.gene44631:CDS |
| AGTTGCAGGGTCAACAAGGT+TGG | 0.506239 | 7.3:-83656449 | MS.gene44631:CDS |
| TTTCGGAAGGCCTTCAAAAG+TGG | 0.513160 | 7.3:-83656404 | MS.gene44631:CDS |
| ACAGAAAGTAAGGATTTGAA+AGG | 0.528561 | 7.3:-83656343 | MS.gene44631:CDS |
| GTTGCAGGGTCAACAAGGTT+GGG | 0.534201 | 7.3:-83656448 | MS.gene44631:CDS |
| TGGATAAGGGTGTTCTTGAT+GGG | 0.534245 | 7.3:-83656531 | MS.gene44631:CDS |
| TGTTCTTGAGAAGCAAGTGA+TGG | 0.541641 | 7.3:-83656302 | MS.gene44631:CDS |
| GAAAGTAAGGATTTGAAAGG+TGG | 0.551682 | 7.3:-83656340 | MS.gene44631:CDS |
| TATTTGACTATGATTGGTGA+AGG | 0.564905 | 7.3:-83656487 | MS.gene44631:CDS |
| TAGGAGTTGCAGGGTCAACA+AGG | 0.623066 | 7.3:-83656453 | MS.gene44631:CDS |
| TTCAAGGAATAGGAGTTGCA+GGG | 0.658447 | 7.3:-83656462 | MS.gene44631:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | TCTAAATATTTGACTATGAT+TGG | - | chr7.3:83656297-83656316 | MS.gene44631:CDS | 20.0% |
| !!! | TATGAAATTGATTTTGGTTT+CGG | - | chr7.3:83656369-83656388 | MS.gene44631:CDS | 20.0% |
| ! | ATTGATAGAGGATTAACTAT+TGG | - | chr7.3:83656420-83656439 | MS.gene44631:CDS | 25.0% |
| !!! | GGAGTATATGAAATTGATTT+TGG | - | chr7.3:83656363-83656382 | MS.gene44631:CDS | 25.0% |
| !!! | TATTGGTTTAACAGAAAGTA+AGG | - | chr7.3:83656437-83656456 | MS.gene44631:CDS | 25.0% |
| ACAGAAAGTAAGGATTTGAA+AGG | - | chr7.3:83656447-83656466 | MS.gene44631:CDS | 30.0% | |
| GAGATTACTTCAATTGATAG+AGG | - | chr7.3:83656408-83656427 | MS.gene44631:CDS | 30.0% | |
| TATTTGACTATGATTGGTGA+AGG | - | chr7.3:83656303-83656322 | MS.gene44631:CDS | 30.0% | |
| ! | TTCAAGCAATTTTTCGTGAA+GGG | - | chr7.3:83656517-83656536 | MS.gene44631:CDS | 30.0% |
| ! | TTTCAAGCAATTTTTCGTGA+AGG | - | chr7.3:83656516-83656535 | MS.gene44631:CDS | 30.0% |
| !!! | AAATTGATTTTGGTTTCGGA+AGG | - | chr7.3:83656373-83656392 | MS.gene44631:CDS | 30.0% |
| ATGATTGGTGAAGGAATTCA+AGG | - | chr7.3:83656312-83656331 | MS.gene44631:CDS | 35.0% | |
| GAAAGTAAGGATTTGAAAGG+TGG | - | chr7.3:83656450-83656469 | MS.gene44631:CDS | 35.0% | |
| ! | TTGAAAGGTGGTGTTGAATT+TGG | - | chr7.3:83656462-83656481 | MS.gene44631:CDS | 35.0% |
| !! | GAAGTAATCTCCACTTTTGA+AGG | + | chr7.3:83656399-83656418 | None:intergenic | 35.0% |
| ATTCAAGGAATAGGAGTTGC+AGG | - | chr7.3:83656327-83656346 | MS.gene44631:CDS | 40.0% | |
| GGTGAAGGAATTCAAGGAAT+AGG | - | chr7.3:83656318-83656337 | MS.gene44631:CDS | 40.0% | |
| TGTTCTTGAGAAGCAAGTGA+TGG | - | chr7.3:83656488-83656507 | MS.gene44631:CDS | 40.0% | |
| TTCAAGGAATAGGAGTTGCA+GGG | - | chr7.3:83656328-83656347 | MS.gene44631:CDS | 40.0% | |
| !! | ATGGATAAGGGTGTTCTTGA+TGG | - | chr7.3:83656258-83656277 | MS.gene44631:CDS | 40.0% |
| !! | TGGATAAGGGTGTTCTTGAT+GGG | - | chr7.3:83656259-83656278 | MS.gene44631:CDS | 40.0% |
| TTTCGGAAGGCCTTCAAAAG+TGG | - | chr7.3:83656386-83656405 | MS.gene44631:CDS | 45.0% | |
| AGTTGCAGGGTCAACAAGGT+TGG | - | chr7.3:83656341-83656360 | MS.gene44631:CDS | 50.0% | |
| GTTGCAGGGTCAACAAGGTT+GGG | - | chr7.3:83656342-83656361 | MS.gene44631:CDS | 50.0% | |
| TAGGAGTTGCAGGGTCAACA+AGG | - | chr7.3:83656337-83656356 | MS.gene44631:CDS | 50.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr7.3 | gene | 83656258 | 83656554 | 83656258 | ID=MS.gene44631 |
| chr7.3 | mRNA | 83656258 | 83656554 | 83656258 | ID=MS.gene44631.t1;Parent=MS.gene44631 |
| chr7.3 | exon | 83656258 | 83656554 | 83656258 | ID=MS.gene44631.t1.exon1;Parent=MS.gene44631.t1 |
| chr7.3 | CDS | 83656258 | 83656554 | 83656258 | ID=cds.MS.gene44631.t1;Parent=MS.gene44631.t1 |
| Gene Sequence |
| Protein sequence |
