AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene48921

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene48921.t1 MTR_4g025730 99.545 440 2 0 1 440 70 509 0.0 907
MS.gene48921.t1 MTR_4g025690 76.351 444 98 4 2 440 72 513 0.0 700
MS.gene48921.t1 MTR_8g104655 53.333 450 181 8 19 440 93 541 7.07e-166 478
MS.gene48921.t1 MTR_7g108905 51.496 468 195 10 1 436 85 552 7.70e-160 463
MS.gene48921.t1 MTR_4g035980 51.082 462 203 9 1 440 79 539 2.22e-155 451
MS.gene48921.t1 MTR_3g033690 50.877 456 206 9 1 440 73 526 3.78e-153 445
MS.gene48921.t1 MTR_3g008640 52.304 434 189 9 23 440 96 527 2.20e-152 443
MS.gene48921.t1 MTR_3g008720 52.074 434 190 9 23 440 96 527 1.63e-151 441
MS.gene48921.t1 MTR_7g053360 52.074 434 190 9 23 440 96 527 1.98e-151 440
MS.gene48921.t1 MTR_3g008700 52.074 434 190 9 23 440 96 527 1.48e-150 438
MS.gene48921.t1 MTR_8g104645 60.882 340 123 5 111 440 33 372 1.07e-148 427
MS.gene48921.t1 MTR_7g050870 50.109 459 202 10 5 440 100 554 7.08e-146 427
MS.gene48921.t1 MTR_4g025670 51.740 431 186 9 26 440 65 489 1.45e-144 421
MS.gene48921.t1 MTR_7g112580 49.443 449 189 10 22 440 102 542 5.80e-143 419
MS.gene48921.t1 MTR_8g023310 49.786 468 192 11 14 440 124 589 8.72e-143 421
MS.gene48921.t1 MTR_7g050980 50.334 449 192 11 17 440 113 555 2.07e-142 418
MS.gene48921.t1 MTR_4g087980 51.025 439 183 9 26 440 86 516 5.14e-142 416
MS.gene48921.t1 MTR_2g044880 50.228 438 191 8 26 440 92 525 3.64e-140 412
MS.gene48921.t1 MTR_8g104580 47.661 449 175 9 19 440 85 500 4.31e-140 410
MS.gene48921.t1 MTR_1g083520 50.794 441 183 10 29 440 93 528 1.89e-138 407
MS.gene48921.t1 MTR_1g088020 48.760 484 192 14 10 440 75 555 8.15e-137 404
MS.gene48921.t1 MTR_7g050950 47.939 461 208 12 5 440 100 553 8.83e-137 404
MS.gene48921.t1 MTR_3g091380 46.537 462 204 9 20 440 138 597 1.29e-136 405
MS.gene48921.t1 MTR_1g054880 49.677 465 182 14 26 440 96 558 1.32e-135 401
MS.gene48921.t1 MTR_1g008140 46.039 467 204 11 21 440 142 607 9.15e-135 400
MS.gene48921.t1 MTR_7g108900 48.077 468 188 13 26 440 95 560 3.85e-133 395
MS.gene48921.t1 MTR_1g088225 48.347 484 192 15 10 440 75 553 1.09e-132 393
MS.gene48921.t1 MTR_7g108875 48.180 467 187 13 26 440 101 564 8.38e-130 386
MS.gene48921.t1 MTR_7g108890 49.417 429 175 9 53 440 135 562 2.02e-129 385
MS.gene48921.t1 MTR_3g010780 47.072 461 205 11 10 440 123 574 2.43e-129 385
MS.gene48921.t1 MTR_8g042910 47.158 475 192 12 23 440 140 612 7.30e-128 383
MS.gene48921.t1 MTR_8g042900 48.980 441 197 13 24 440 145 581 4.62e-127 380
MS.gene48921.t1 MTR_4g087970 45.475 442 207 8 26 440 83 517 4.37e-126 375
MS.gene48921.t1 MTR_4g124520 48.515 404 187 10 53 440 123 521 3.84e-122 365
MS.gene48921.t1 MTR_0458s0030 64.639 263 89 3 181 440 53 314 2.00e-121 356
MS.gene48921.t1 MTR_1g088040 64.639 263 89 3 181 440 53 314 2.00e-121 356
MS.gene48921.t1 MTR_2g044810 44.766 449 221 11 12 440 93 534 3.47e-120 361
MS.gene48921.t1 MTR_4g124550 43.478 460 221 10 17 440 137 593 2.82e-119 360
MS.gene48921.t1 MTR_3g010770 55.589 331 135 6 121 440 246 575 3.59e-118 357
MS.gene48921.t1 MTR_1g086210 40.812 468 228 10 21 440 103 569 1.75e-113 345
MS.gene48921.t1 MTR_0618s0020 66.520 227 73 2 217 440 2 228 3.36e-111 327
MS.gene48921.t1 MTR_1g083510 39.959 483 239 13 7 439 101 582 7.70e-104 320
MS.gene48921.t1 MTR_8g023150 44.581 406 204 8 55 440 178 582 8.07e-103 318
MS.gene48921.t1 MTR_1g086190 40.132 456 232 12 24 440 110 563 3.64e-102 315
MS.gene48921.t1 MTR_7g110490 39.700 466 225 10 24 435 106 569 2.02e-100 311
MS.gene48921.t1 MTR_7g110510 39.700 466 225 10 24 435 106 569 2.02e-100 311
MS.gene48921.t1 MTR_7g110560 40.308 454 226 13 24 440 104 549 2.96e-100 310
MS.gene48921.t1 MTR_3g095920 41.333 450 218 14 30 439 120 563 4.32e-99 307
MS.gene48921.t1 MTR_2g044660 47.605 334 166 7 111 440 304 632 7.65e-99 309
MS.gene48921.t1 MTR_1g083470 38.147 464 227 13 19 436 117 566 3.10e-98 306
MS.gene48921.t1 MTR_1g083500 38.147 464 227 13 19 436 117 566 3.10e-98 306
MS.gene48921.t1 MTR_7g110440 37.792 471 237 10 24 440 106 574 8.11e-98 305
MS.gene48921.t1 MTR_8g093460 40.826 436 216 12 30 429 111 540 3.05e-97 303
MS.gene48921.t1 MTR_7g112590 37.988 487 252 14 1 439 98 582 1.78e-96 301
MS.gene48921.t1 MTR_4g005170 38.106 454 241 13 19 436 122 571 1.38e-95 300
MS.gene48921.t1 MTR_2g044810 55.378 251 108 3 193 440 216 465 1.20e-94 293
MS.gene48921.t1 MTR_1g083490 37.528 453 245 12 19 436 122 571 1.71e-94 296
MS.gene48921.t1 MTR_4g005180 36.638 464 234 13 19 436 104 553 1.33e-91 288
MS.gene48921.t1 MTR_4g120290 37.418 457 241 13 24 439 119 571 1.27e-89 283
MS.gene48921.t1 MTR_4g120280 36.980 457 243 12 24 439 119 571 2.73e-88 280
MS.gene48921.t1 MTR_2g436050 38.000 450 243 14 24 439 116 563 3.76e-87 276
MS.gene48921.t1 MTR_2g436040 35.374 441 256 10 24 437 105 543 3.96e-87 278
MS.gene48921.t1 MTR_8g088610 43.917 337 169 10 113 437 45 373 1.06e-84 264
MS.gene48921.t1 MTR_7g110420 34.979 486 242 12 24 440 106 586 9.96e-82 263
MS.gene48921.t1 MTR_0458s0010 38.525 488 199 18 10 440 75 518 1.02e-79 256
MS.gene48921.t1 MTR_5g083620 40.762 341 189 8 110 440 19 356 8.56e-79 248
MS.gene48921.t1 MTR_7g110430 33.333 468 228 9 24 440 106 540 8.62e-79 254
MS.gene48921.t1 MTR_7g107180 41.139 316 170 7 131 440 2 307 1.69e-72 231
MS.gene48921.t1 MTR_2g105560 40.705 312 167 7 135 440 40 339 1.87e-71 229
MS.gene48921.t1 MTR_7g111560 31.788 453 271 14 17 440 113 556 8.11e-67 223
MS.gene48921.t1 MTR_2g039530 40.594 303 162 8 110 406 28 318 8.65e-58 193
MS.gene48921.t1 MTR_6g024165 37.072 321 176 10 121 429 28 334 1.34e-52 180
MS.gene48921.t1 MTR_6g088810 39.735 302 152 9 144 429 90 377 9.16e-52 179
MS.gene48921.t1 MTR_6g012800 35.644 303 167 9 136 423 6 295 5.69e-50 172
MS.gene48921.t1 MTR_8g102380 38.065 310 162 10 137 429 81 377 9.33e-50 173
MS.gene48921.t1 MTR_6g042660 34.545 275 157 7 136 402 37 296 1.16e-45 161
MS.gene48921.t1 MTR_7g011530 34.091 308 176 8 138 429 8 304 1.29e-45 160
MS.gene48921.t1 MTR_5g083590 31.529 314 158 7 134 440 5 268 9.21e-45 157
MS.gene48921.t1 MTR_1g086540 32.919 322 189 8 121 429 37 344 1.76e-44 159
MS.gene48921.t1 MTR_7g092780 31.548 336 198 8 108 429 70 387 5.54e-44 158
MS.gene48921.t1 MTR_2g033970 31.746 315 192 8 125 429 45 346 9.30e-41 149
MS.gene48921.t1 MTR_8g102380 38.976 254 128 8 137 373 81 324 1.89e-40 147
MS.gene48921.t1 MTR_6g042690 32.979 282 161 7 134 402 37 303 1.97e-39 145
MS.gene48921.t1 MTR_1g033050 30.892 314 186 8 131 433 13 306 2.47e-39 144
MS.gene48921.t1 MTR_5g095490 33.225 307 170 8 142 429 67 357 4.80e-38 142
MS.gene48921.t1 MTR_5g093970 32.787 305 182 9 136 432 43 332 2.70e-37 139
MS.gene48921.t1 MTR_4g124740 31.366 322 184 10 124 429 39 339 5.54e-36 135
MS.gene48921.t1 MTR_5g088300 30.537 298 189 6 136 431 37 318 7.47e-35 132
MS.gene48921.t1 MTR_2g436600 31.803 305 174 11 136 429 55 336 2.30e-34 131
MS.gene48921.t1 MTR_7g102600 31.169 308 185 7 138 431 101 395 3.13e-34 132
MS.gene48921.t1 MTR_5g012600 29.808 312 192 8 136 432 63 362 2.06e-33 129
MS.gene48921.t1 MTR_5g088280 29.195 298 193 6 136 431 39 320 1.61e-31 123
MS.gene48921.t1 MTR_4g074290 29.063 320 193 11 125 429 61 361 1.92e-31 124
MS.gene48921.t1 MTR_8g036225 27.541 305 195 6 136 429 33 322 3.32e-31 122
MS.gene48921.t1 MTR_5g088290 29.605 304 185 9 136 431 48 330 4.67e-31 122
MS.gene48921.t1 MTR_2g073390 26.975 367 230 13 81 429 15 361 7.78e-31 122
MS.gene48921.t1 MTR_4g051810 30.233 301 187 8 136 431 37 319 1.39e-30 120
MS.gene48921.t1 MTR_0009s0350 28.981 314 201 9 125 429 61 361 1.48e-29 119
MS.gene48921.t1 MTR_0009s0340 28.981 314 201 9 125 429 61 361 1.48e-29 119
MS.gene48921.t1 MTR_6g025590 36.158 177 100 5 121 286 36 210 2.39e-22 95.9
MS.gene48921.t1 MTR_0408s0030 37.895 95 52 1 308 402 36 123 1.47e-16 77.0
MS.gene48921.t1 MTR_2g436070 30.000 230 132 9 2 204 94 321 2.40e-15 77.8
MS.gene48921.t1 MTR_6g024200 34.591 159 83 7 273 429 137 276 3.03e-14 73.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene48921.t1 AT2G45220 70.562 445 122 6 1 440 71 511 0.0 642
MS.gene48921.t1 AT5G51490 53.219 466 188 10 1 440 75 536 5.03e-165 475
MS.gene48921.t1 AT5G51500 52.371 464 195 9 1 440 79 540 6.03e-157 455
MS.gene48921.t1 AT4G00190 51.339 448 195 7 1 440 42 474 8.84e-154 444
MS.gene48921.t1 AT3G47400 50.858 466 203 9 1 440 129 594 2.63e-153 447
MS.gene48921.t1 AT1G23200 50.000 460 179 8 25 440 102 554 6.87e-150 437
MS.gene48921.t1 AT3G59010 51.389 432 183 5 29 440 104 528 5.58e-142 416
MS.gene48921.t1 AT1G11580 51.136 440 194 9 22 440 118 557 9.75e-142 417
MS.gene48921.t1 AT1G11580 51.136 440 194 9 22 440 139 578 2.28e-141 416
MS.gene48921.t1 AT5G53370 48.337 451 202 7 21 440 135 585 1.78e-140 414
MS.gene48921.t1 AT3G49220 48.889 450 199 9 21 440 148 596 4.77e-140 414
MS.gene48921.t1 AT5G53370 48.337 451 202 7 21 440 171 621 5.54e-140 414
MS.gene48921.t1 AT3G49220 48.889 450 199 9 21 440 193 641 1.77e-139 414
MS.gene48921.t1 AT3G14310 48.421 475 189 11 20 440 120 592 2.19e-138 409
MS.gene48921.t1 AT4G02320 47.661 449 206 10 20 440 71 518 1.17e-136 402
MS.gene48921.t1 AT3G43270 48.107 449 196 9 26 440 80 525 1.66e-136 402
MS.gene48921.t1 AT1G53830 48.718 468 190 11 20 440 123 587 2.44e-136 404
MS.gene48921.t1 AT3G05620 47.034 472 207 11 2 440 80 541 7.95e-136 401
MS.gene48921.t1 AT3G60730 45.833 456 206 8 24 440 66 519 1.82e-133 394
MS.gene48921.t1 AT2G43050 49.296 426 193 9 29 440 100 516 5.12e-131 388
MS.gene48921.t1 AT4G33220 45.887 462 197 9 26 440 68 523 7.91e-131 388
MS.gene48921.t1 AT4G02300 45.598 443 216 8 23 440 90 532 1.82e-128 382
MS.gene48921.t1 AT2G47550 49.784 462 183 13 26 440 99 558 4.24e-128 382
MS.gene48921.t1 AT4G02330 47.964 442 172 12 53 440 134 571 8.95e-128 382
MS.gene48921.t1 AT1G02810 48.552 449 162 13 55 440 135 577 2.33e-125 375
MS.gene48921.t1 AT4G33220 54.491 334 144 5 113 440 71 402 2.49e-124 367
MS.gene48921.t1 AT2G26450 44.592 453 222 13 14 440 162 611 2.41e-119 361
MS.gene48921.t1 AT5G20860 44.444 432 214 9 30 440 112 538 2.12e-118 356
MS.gene48921.t1 AT5G04960 44.037 436 219 9 25 438 130 562 3.60e-118 357
MS.gene48921.t1 AT3G10710 45.977 435 202 10 30 440 136 561 4.78e-116 351
MS.gene48921.t1 AT5G49180 46.247 413 186 11 55 436 159 566 2.42e-114 347
MS.gene48921.t1 AT3G05610 41.060 453 234 12 19 440 115 565 3.81e-114 349
MS.gene48921.t1 AT3G10720 43.750 480 212 13 16 440 139 615 5.81e-114 347
MS.gene48921.t1 AT2G26440 42.889 450 222 10 23 440 101 547 1.95e-113 344
MS.gene48921.t1 AT4G33230 43.103 464 226 12 12 440 146 606 6.84e-112 342
MS.gene48921.t1 AT5G04970 42.827 481 215 13 16 440 144 620 1.56e-110 339
MS.gene48921.t1 AT5G27870 39.468 451 242 11 19 440 114 562 3.80e-110 341
MS.gene48921.t1 AT1G11590 43.373 415 224 7 37 440 110 524 2.88e-107 327
MS.gene48921.t1 AT3G06830 39.741 463 230 13 19 438 110 566 5.11e-107 328
MS.gene48921.t1 AT4G03930 43.133 415 225 7 37 440 110 524 6.98e-107 326
MS.gene48921.t1 AT3G27980 43.284 402 202 6 55 440 106 497 2.28e-106 324
MS.gene48921.t1 AT3G10720 57.692 260 106 3 184 440 1 259 2.95e-104 310
MS.gene48921.t1 AT1G53840 40.948 464 223 13 14 434 126 581 6.75e-103 318
MS.gene48921.t1 AT4G15980 47.278 349 174 7 99 438 353 700 6.20e-102 319
MS.gene48921.t1 AT4G15980 47.278 349 174 7 99 438 363 710 1.46e-101 318
MS.gene48921.t1 AT3G14300 41.441 444 214 11 24 429 523 958 2.63e-99 318
MS.gene48921.t1 AT5G64640 39.865 444 222 13 30 433 164 602 3.34e-95 298
MS.gene48921.t1 AT5G09760 40.367 436 218 13 30 428 116 546 8.33e-93 291
MS.gene48921.t1 AT5G09760 40.367 436 218 13 30 428 142 572 1.70e-92 291
MS.gene48921.t1 AT5G20860 40.955 398 182 8 30 406 112 477 2.18e-92 288
MS.gene48921.t1 AT2G47030 43.678 348 175 8 112 440 243 588 7.11e-83 266
MS.gene48921.t1 AT2G47040 43.768 345 176 8 112 440 253 595 1.74e-80 260
MS.gene48921.t1 AT3G62170 41.292 356 180 8 112 440 235 588 8.73e-79 255
MS.gene48921.t1 AT1G11370 49.187 246 119 4 133 372 43 288 1.17e-74 235
MS.gene48921.t1 AT5G19730 37.134 307 167 8 137 429 87 381 2.07e-48 170
MS.gene48921.t1 AT3G29090 35.789 285 162 5 131 402 2 278 1.41e-47 166
MS.gene48921.t1 AT1G05310 33.038 339 195 9 105 429 68 388 4.45e-44 159
MS.gene48921.t1 AT3G17060 31.861 317 189 8 125 429 35 336 5.21e-44 157
MS.gene48921.t1 AT2G36700 32.667 300 184 5 138 429 44 333 1.33e-43 156
MS.gene48921.t1 AT2G36710 33.019 318 184 11 133 434 86 390 1.59e-40 149
MS.gene48921.t1 AT1G44980 43.617 188 101 3 114 296 54 241 1.63e-40 145
MS.gene48921.t1 AT2G21610 30.100 299 187 7 136 429 51 332 2.28e-38 142
MS.gene48921.t1 AT2G21610 30.100 299 187 7 136 429 56 337 2.45e-38 142
MS.gene48921.t1 AT5G18990 34.035 285 166 6 131 402 24 299 3.60e-38 141
MS.gene48921.t1 AT5G55590 33.333 309 179 9 136 429 82 378 2.55e-37 140
MS.gene48921.t1 AT2G19150 31.579 323 191 10 128 436 33 339 1.41e-36 137
MS.gene48921.t1 AT3G24130 32.155 283 170 6 133 402 31 304 7.39e-36 135
MS.gene48921.t1 AT5G47500 28.267 329 198 8 116 429 52 357 1.40e-35 135
MS.gene48921.t1 AT5G07430 29.067 375 229 14 71 429 2 355 2.38e-35 134
MS.gene48921.t1 AT5G26810 30.793 328 197 10 122 431 20 335 5.92e-35 133
MS.gene48921.t1 AT5G55590 35.573 253 139 8 136 373 82 325 1.65e-34 131
MS.gene48921.t1 AT3G49220 35.616 219 113 7 21 212 193 410 5.78e-31 124
MS.gene48921.t1 AT2G47280 30.421 309 184 8 136 429 35 327 9.16e-31 121
MS.gene48921.t1 AT2G19150 32.171 258 152 8 128 373 33 279 1.54e-29 117
MS.gene48921.t1 AT5G07420 27.859 341 214 10 107 429 29 355 6.55e-29 117
MS.gene48921.t1 AT5G61680 27.885 312 200 9 129 429 61 358 2.56e-26 109
MS.gene48921.t1 AT5G07410 26.163 344 227 10 99 429 26 355 2.86e-26 109
MS.gene48921.t1 AT1G69940 25.946 370 244 12 75 429 1 355 1.74e-25 107

Find 99 sgRNAs with CRISPR-Local

Find 201 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GGGACAGTTGATTTCATCTT+TGG 0.221019 4.3:-72838578 MS.gene48921:CDS
GAATAATAATTCATAATTCT+AGG 0.226049 4.3:-72838448 MS.gene48921:CDS
TACAGAGAATGTAACATCTA+TGG 0.256911 4.3:-72838599 MS.gene48921:CDS
GAATTATTATTCCAGTGTTT+TGG 0.305948 4.3:+72838459 None:intergenic
CAGGTTCATCCACTTCAAAT+AGG 0.323866 4.3:-72838235 MS.gene48921:CDS
TTCATGAAAACTGTTATTGA+TGG 0.343065 4.3:-72838344 MS.gene48921:CDS
CAAGCTGTTGCATTTCGTTC+CGG 0.356258 4.3:-72838701 MS.gene48921:CDS
ACCGTTGGGAATTTCATTGC+CGG 0.366873 4.3:-72838158 MS.gene48921:CDS
GAATATGCTAATACTGGTTC+AGG 0.372283 4.3:-72838254 MS.gene48921:CDS
ATTTCATTGCCGGTAATTCA+TGG 0.379520 4.3:-72838148 MS.gene48921:CDS
CATCTTTGTAACTTGGTTGT+TGG 0.388751 4.3:+72840757 None:intergenic
GTTTCCTATCCATGAATTAC+CGG 0.395370 4.3:+72838139 None:intergenic
GAGCTGTACTAAGCCATGTT+TGG 0.396609 4.3:+72840907 None:intergenic
TATGCTGAATATGCTAATAC+TGG 0.408188 4.3:-72838260 MS.gene48921:CDS
AGCTGTACTAAGCCATGTTT+GGG 0.411544 4.3:+72840908 None:intergenic
CGTCGTTGCTAAGGATGGTT+CGG 0.414368 4.3:-72840661 MS.gene48921:CDS
TTGTAACTTGGTTGTTGGTA+AGG 0.414505 4.3:+72840762 None:intergenic
TTCTAGGGTTTCCGCTCAAT+CGG 0.419321 4.3:-72838432 MS.gene48921:CDS
AGGTTCATCCACTTCAAATA+GGG 0.421888 4.3:-72838234 MS.gene48921:CDS
TTTGCAGCTGCTACTGGTGA+TGG 0.425142 4.3:-72838776 MS.gene48921:intron
GTCGTTGCTAAGGATGGTTC+GGG 0.434190 4.3:-72840660 MS.gene48921:CDS
CATGGGTTAAACCTGGTGAT+AGG 0.443867 4.3:-72840725 MS.gene48921:CDS
AACTTCTTCCGCGGCTTCGA+AGG 0.447444 4.3:-72840691 MS.gene48921:CDS
AATAATAATTCATAATTCTA+GGG 0.452751 4.3:-72838447 MS.gene48921:CDS
TCGTTCCGGCTCCGATCTAT+CGG 0.457003 4.3:-72838687 MS.gene48921:CDS
GGCTAATGTCGTCGTTGCTA+AGG 0.459522 4.3:-72840670 MS.gene48921:CDS
CCACTTCAAATAGGGTTACA+TGG 0.460645 4.3:-72838226 MS.gene48921:CDS
CAAGCTTCACCTTTCACCGT+TGG 0.471593 4.3:-72838173 MS.gene48921:CDS
AAGCTTCACCTTTCACCGTT+GGG 0.472202 4.3:-72838172 MS.gene48921:CDS
ATGAAATTCCCAACGGTGAA+AGG 0.479388 4.3:+72838164 None:intergenic
CAACAACCAAGTTACAAAGA+TGG 0.480845 4.3:-72840756 MS.gene48921:CDS
CCCTGCTGGTTGGTTACCAT+GGG 0.482029 4.3:-72838312 MS.gene48921:CDS
TCCTTGCGCGGTAACTGTGA+TGG 0.482943 4.3:+72838496 None:intergenic
ACAGGCGTGCCATTCACCTC+TGG 0.485673 4.3:-72838116 MS.gene48921:CDS
CTGTTAAATCGTACCTTGGT+AGG 0.486089 4.3:-72838394 MS.gene48921:CDS
CCATGTAACCCTATTTGAAG+TGG 0.490411 4.3:+72838226 None:intergenic
TCTTGTGGGCTACGGCACTT+TGG 0.492867 4.3:+72841023 None:intergenic
GGAAATCCATCTTTGTAACT+TGG 0.499245 4.3:+72840750 None:intergenic
ATTGCCGGTAATTCATGGAT+AGG 0.500552 4.3:-72838143 MS.gene48921:CDS
TCCCATGGTAACCAACCAGC+AGG 0.502238 4.3:+72838311 None:intergenic
ACAGAGAATGTAACATCTAT+GGG 0.505050 4.3:-72838598 MS.gene48921:CDS
ACCCTGCTGGTTGGTTACCA+TGG 0.509749 4.3:-72838313 MS.gene48921:CDS
GGTACGATTTAACAGAGCTA+GGG 0.515662 4.3:+72838402 None:intergenic
GATGCTGCACCAAGTGGTAG+TGG 0.515780 4.3:-72840609 MS.gene48921:CDS
ATGTTGGTGGGTGATGGTAT+TGG 0.525394 4.3:-72840519 MS.gene48921:CDS
TCAAATAGGGTTACATGGAA+AGG 0.533882 4.3:-72838221 MS.gene48921:CDS
AGGTACGATTTAACAGAGCT+AGG 0.534156 4.3:+72838401 None:intergenic
ATTGGTAAAACCATAATCAC+TGG 0.538242 4.3:-72840501 MS.gene48921:CDS
TCACCAGGTTTAACCCATGT+TGG 0.540325 4.3:+72840729 None:intergenic
TGAAGAAGTTTCCTATCACC+AGG 0.540939 4.3:+72840714 None:intergenic
ATCACTGGTAGCAAAAGTGT+TGG 0.542034 4.3:-72840486 MS.gene48921:CDS
ACCATCACAGTTACCGCGCA+AGG 0.543990 4.3:-72838497 MS.gene48921:CDS
ACAAAGATGGATTTCCAACA+TGG 0.557740 4.3:-72840743 MS.gene48921:CDS
AATGTCGTCGTTGCTAAGGA+TGG 0.559942 4.3:-72840666 MS.gene48921:CDS
ATCACATTCAGAAACACAGC+TGG 0.561597 4.3:-72838737 MS.gene48921:CDS
AATGTCATGTTGGTGGGTGA+TGG 0.561648 4.3:-72840525 MS.gene48921:CDS
ACTGGTAGCAAAAGTGTTGG+AGG 0.563435 4.3:-72840483 MS.gene48921:CDS
TAAAGCAAAGAATGTCATGT+TGG 0.567010 4.3:-72840535 MS.gene48921:CDS
TTTGTAAAAGACCGATAGAT+CGG 0.567687 4.3:+72838676 None:intergenic
AATCGTACCTTGGTAGGCCG+TGG 0.576253 4.3:-72838388 MS.gene48921:CDS
AAAGACCGATAGATCGGAGC+CGG 0.577312 4.3:+72838682 None:intergenic
CACAATATGAATCCCAAACA+TGG 0.578929 4.3:-72840920 MS.gene48921:CDS
GCTTAAAGTCCAGAGGTGAA+TGG 0.586097 4.3:+72838107 None:intergenic
ACTGATCCAAACCAAAACAC+TGG 0.586539 4.3:-72838470 MS.gene48921:CDS
TTTCCAACATGGGTTAAACC+TGG 0.589586 4.3:-72840732 MS.gene48921:CDS
GTTACATTATTAGACAACAA+AGG 0.594044 4.3:+72840822 None:intergenic
ACTTTCCGTTCAGCAACCGT+TGG 0.594941 4.3:-72840450 MS.gene48921:intron
AGCAAAGAATGTCATGTTGG+TGG 0.597389 4.3:-72840532 MS.gene48921:CDS
TATGTGATATATGTGAAGGC+TGG 0.599695 4.3:-72840582 MS.gene48921:CDS
GGGCAAGTACACGACGGTGA+AGG 0.602626 4.3:-72840640 MS.gene48921:CDS
GCTGGTTGGTTACCATGGGA+TGG 0.604546 4.3:-72838308 MS.gene48921:CDS
CTGCACCAAGTGGTAGTGGA+AGG 0.608150 4.3:-72840605 MS.gene48921:CDS
TGGATCAGTTCTTCCTTGCG+CGG 0.608915 4.3:+72838484 None:intergenic
AAAGCAAAATTACCATCCCA+TGG 0.612268 4.3:+72838296 None:intergenic
CACGACGGTGAAGGCCGCGA+CGG 0.612289 4.3:-72840631 MS.gene48921:CDS
GACATTAGCCTTCGAAGCCG+CGG 0.622925 4.3:+72840683 None:intergenic
TAACACCTTGTCACTCAACA+AGG 0.624473 4.3:-72840787 MS.gene48921:CDS
AGCTCTGTTAAATCGTACCT+TGG 0.625217 4.3:-72838398 MS.gene48921:CDS
ACTTACCAACGGTTGCTGAA+CGG 0.626940 4.3:+72840445 None:intergenic
CAAAGATGGATTTCCAACAT+GGG 0.627787 4.3:-72840742 MS.gene48921:CDS
GAGGAAAATACACATGCACT+TGG 0.629768 4.3:-72841047 MS.gene48921:CDS
GCAAAGAATGTCATGTTGGT+GGG 0.630792 4.3:-72840531 MS.gene48921:CDS
ACTTCTTCAAACTTCTTCCG+CGG 0.630906 4.3:-72840700 MS.gene48921:CDS
AGGGTTTAAATCCGATTGAG+CGG 0.635907 4.3:+72838421 None:intergenic
ACATACCTTCCACTACCACT+TGG 0.638708 4.3:+72840600 None:intergenic
GGTAGCAAAAGTGTTGGAGG+TGG 0.640198 4.3:-72840480 MS.gene48921:CDS
GTGAAGCACTAGTAAGAACA+TGG 0.641780 4.3:+72838195 None:intergenic
AAGGTATGTGATATATGTGA+AGG 0.653633 4.3:-72840586 MS.gene48921:CDS
AGGAACCTTGTTGAGTGACA+AGG 0.656613 4.3:+72840782 None:intergenic
GCGACGGATGCTGCACCAAG+TGG 0.664715 4.3:-72840615 MS.gene48921:CDS
GGTTGCTGAACGGAAAGTAG+TGG 0.670011 4.3:+72840455 None:intergenic
CCCATGGTAACCAACCAGCA+GGG 0.670836 4.3:+72838312 None:intergenic
ACCGGCAATGAAATTCCCAA+CGG 0.671958 4.3:+72838157 None:intergenic
AATTCATGGATAGGAAACAC+AGG 0.687418 4.3:-72838134 MS.gene48921:CDS
AAGGAACGCTTAAAGTCCAG+AGG 0.689657 4.3:+72838100 None:intergenic
ACTTGGTGCAGCATCCGTCG+CGG 0.690402 4.3:+72840617 None:intergenic
AGACATAGTTTGTGACACCA+AGG 0.696122 4.3:+72840847 None:intergenic
TGGTTCGGGCAAGTACACGA+CGG 0.758627 4.3:-72840646 MS.gene48921:CDS
TTTCATCTTTGGAAACGCCG+CGG 0.774990 4.3:-72838567 MS.gene48921:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TAAATAAATATTTAAATTAA+TGG + chr4.3:72839059-72839078 None:intergenic 0.0%
!! AAATAAATAATTATATCAGT+AGG - chr4.3:72839327-72839346 MS.gene48921:intron 10.0%
!! AAATATAACAAATAACTTAA+AGG + chr4.3:72839209-72839228 None:intergenic 10.0%
!! AATAAATAATTATATCAGTA+GGG - chr4.3:72839328-72839347 MS.gene48921:intron 10.0%
!! AATAATAATTCATAATTCTA+GGG - chr4.3:72840730-72840749 MS.gene48921:CDS 10.0%
!! ATTTCTTAATCATTAAATTA+AGG + chr4.3:72839674-72839693 None:intergenic 10.0%
!! TTTCTTAATCATTAAATTAA+GGG + chr4.3:72839673-72839692 None:intergenic 10.0%
!! ACTTTAACTAATTTCTTAAT+TGG - chr4.3:72840047-72840066 MS.gene48921:intron 15.0%
!! ATAAATAATTATATCAGTAG+GGG - chr4.3:72839329-72839348 MS.gene48921:intron 15.0%
!! GAATAATAATTCATAATTCT+AGG - chr4.3:72840729-72840748 MS.gene48921:CDS 15.0%
!! GAATAATTATTTATATCCTT+TGG + chr4.3:72839539-72839558 None:intergenic 15.0%
!! TTAAGATAATATATCATAGA+AGG + chr4.3:72839610-72839629 None:intergenic 15.0%
!! TTAAGGAAAAATATTAATCT+AGG + chr4.3:72839993-72840012 None:intergenic 15.0%
!! TTCAATTAAATCAATTATTG+TGG + chr4.3:72839705-72839724 None:intergenic 15.0%
!! TTTAATTAAAAGAAGAATGT+GGG - chr4.3:72840146-72840165 MS.gene48921:intron 15.0%
!!! ATATTTACAAAAAAAAGTGT+CGG - chr4.3:72839167-72839186 MS.gene48921:intron 15.0%
!!! ATTACTTTAAAATCTCTTTA+CGG - chr4.3:72839583-72839602 MS.gene48921:intron 15.0%
!!! ATTCAATTCATTATGTTTTT+GGG + chr4.3:72839741-72839760 None:intergenic 15.0%
!!! TATTCAATTCATTATGTTTT+TGG + chr4.3:72839742-72839761 None:intergenic 15.0%
!! AAAATTAATTTAACCCTAGA+TGG + chr4.3:72839145-72839164 None:intergenic 20.0%
!! CTAGTTTATGATTTAGTTAT+TGG + chr4.3:72838906-72838925 None:intergenic 20.0%
!! CTTTAATTAAAAGAAGAATG+TGG - chr4.3:72840145-72840164 MS.gene48921:intron 20.0%
!! GAAAATTACTTATGAATGAT+AGG - chr4.3:72839094-72839113 MS.gene48921:intron 20.0%
!! GAATAAGACTATAATAAAGT+TGG + chr4.3:72840114-72840133 None:intergenic 20.0%
!! TATGATTTGAATCATTTACA+TGG - chr4.3:72839800-72839819 MS.gene48921:intron 20.0%
!! TTAATTAAAAGAAGAATGTG+GGG - chr4.3:72840147-72840166 MS.gene48921:intron 20.0%
!! TTATTAAGTAATGGTATACA+TGG + chr4.3:72840181-72840200 None:intergenic 20.0%
!! TTCAACTGATTATTAAGTAA+TGG + chr4.3:72840190-72840209 None:intergenic 20.0%
!!! AATGCAACTGTTTTTTTATT+AGG - chr4.3:72840317-72840336 MS.gene48921:intron 20.0%
!!! ACTTATTTTATTTCCATCTA+GGG - chr4.3:72839129-72839148 MS.gene48921:intron 20.0%
!!! AGATTTTAAAGTAATCACAA+TGG + chr4.3:72839578-72839597 None:intergenic 20.0%
!!! ATCATAGTCTAAAACTTTAT+TGG + chr4.3:72839786-72839805 None:intergenic 20.0%
!!! ATTTGTGAACTTTTCTTTAA+GGG + chr4.3:72838799-72838818 None:intergenic 20.0%
!!! CACATTCTTCTTTTAATTAA+AGG + chr4.3:72840147-72840166 None:intergenic 20.0%
!!! TACTTATTTTATTTCCATCT+AGG - chr4.3:72839128-72839147 MS.gene48921:intron 20.0%
!!! TATTTGTGAACTTTTCTTTA+AGG + chr4.3:72838800-72838819 None:intergenic 20.0%
!!! TGCATAAGATTTTTCAATAA+AGG + chr4.3:72838936-72838955 None:intergenic 20.0%
!!! TTTATAGCTTTGAATTAAAC+CGG + chr4.3:72838985-72839004 None:intergenic 20.0%
! AATCTTGAAACATGCAAAAA+TGG - chr4.3:72838298-72838317 MS.gene48921:CDS 25.0%
! ACATAATGAATTGAATATCG+TGG - chr4.3:72839745-72839764 MS.gene48921:intron 25.0%
! ACTTATGAATGATAGGTAAA+CGG - chr4.3:72839101-72839120 MS.gene48921:intron 25.0%
! ATTATCTACTTTCTTACTCA+AGG + chr4.3:72839513-72839532 None:intergenic 25.0%
! CATATAGGAAACTATATATG+CGG + chr4.3:72840020-72840039 None:intergenic 25.0%
! CATATATAGTTTCCTATATG+TGG - chr4.3:72840020-72840039 MS.gene48921:intron 25.0%
! GTTACATTATTAGACAACAA+AGG + chr4.3:72838358-72838377 None:intergenic 25.0%
! TATGTAGCTTTCAAAAATGA+AGG - chr4.3:72839839-72839858 MS.gene48921:intron 25.0%
! TTCATGAAAACTGTTATTGA+TGG - chr4.3:72840833-72840852 MS.gene48921:CDS 25.0%
! TTTAGTAGTCAAATCTTTAG+AGG - chr4.3:72839912-72839931 MS.gene48921:intron 25.0%
!! CAGGTAAGAACTTTTTAATA+AGG + chr4.3:72838845-72838864 None:intergenic 25.0%
!! GAATTATTATTCCAGTGTTT+TGG + chr4.3:72840721-72840740 None:intergenic 25.0%
!! TGAATGAACTTTTCTTTTCA+TGG + chr4.3:72838874-72838893 None:intergenic 25.0%
!!! AAAAATGGATTTTATGACCT+TGG - chr4.3:72838313-72838332 MS.gene48921:CDS 25.0%
!!! TTTTACAAATGTAGCTTTGA+AGG - chr4.3:72840515-72840534 MS.gene48921:CDS 25.0%
AAGGGATAAAAAAAGCTGTA+CGG + chr4.3:72838781-72838800 None:intergenic 30.0%
AAGGTATGTGATATATGTGA+AGG - chr4.3:72838591-72838610 MS.gene48921:CDS 30.0%
AAGTATTAAAAAACCTAGCG+TGG + chr4.3:72840373-72840392 None:intergenic 30.0%
ACAGAGAATGTAACATCTAT+GGG - chr4.3:72840579-72840598 MS.gene48921:CDS 30.0%
ACTATATATGCGGTTCATTA+AGG + chr4.3:72840010-72840029 None:intergenic 30.0%
ATTGGTAAAACCATAATCAC+TGG - chr4.3:72838676-72838695 MS.gene48921:CDS 30.0%
CTTATGAATGATAGGTAAAC+GGG - chr4.3:72839102-72839121 MS.gene48921:intron 30.0%
CTTTAGATTTGTGTGGTATA+TGG - chr4.3:72839456-72839475 MS.gene48921:intron 30.0%
GAAAGTAGATAATAGACCAA+AGG - chr4.3:72839520-72839539 MS.gene48921:intron 30.0%
GGCTTTCTATATGTTATAGT+CGG + chr4.3:72839946-72839965 None:intergenic 30.0%
TAAAGCAAAGAATGTCATGT+TGG - chr4.3:72838642-72838661 MS.gene48921:CDS 30.0%
TACAGAGAATGTAACATCTA+TGG - chr4.3:72840578-72840597 MS.gene48921:CDS 30.0%
TATGCTGAATATGCTAATAC+TGG - chr4.3:72840917-72840936 MS.gene48921:CDS 30.0%
TGAGAAGCTATGTATAACAA+CGG - chr4.3:72839373-72839392 MS.gene48921:intron 30.0%
TGTTAAGGTTGCATATCTTA+TGG - chr4.3:72839410-72839429 MS.gene48921:intron 30.0%
TTATGAATGATAGGTAAACG+GGG - chr4.3:72839103-72839122 MS.gene48921:intron 30.0%
TTTGTAAAAGACCGATAGAT+CGG + chr4.3:72840504-72840523 None:intergenic 30.0%
! AATCTAGGATTGATTCTAGA+GGG + chr4.3:72839978-72839997 None:intergenic 30.0%
! GTTAAAGTGTTACCACATAT+AGG + chr4.3:72840035-72840054 None:intergenic 30.0%
! TAATAAAGTTGGTCCTACAT+TGG + chr4.3:72840103-72840122 None:intergenic 30.0%
! TAATCTAGGATTGATTCTAG+AGG + chr4.3:72839979-72839998 None:intergenic 30.0%
! TGCACTTTGTGTATTTTGTT+TGG + chr4.3:72838237-72838256 None:intergenic 30.0%
!! ATTTTGTTTGGCTAAGCTTT+TGG + chr4.3:72838225-72838244 None:intergenic 30.0%
!! GTATTGACTTTAGATTTGTG+TGG - chr4.3:72839449-72839468 MS.gene48921:intron 30.0%
!! GTTCTTGAATATTTTTGCCA+CGG + chr4.3:72840809-72840828 None:intergenic 30.0%
!! TTACTAGAAGTACTTACCAA+CGG + chr4.3:72838746-72838765 None:intergenic 30.0%
!!! ATTATTCCAGTGTTTTGGTT+TGG + chr4.3:72840716-72840735 None:intergenic 30.0%
AAAGCAAAATTACCATCCCA+TGG + chr4.3:72840884-72840903 None:intergenic 35.0%
AAGATATGCAACCTTAACAC+CGG + chr4.3:72839409-72839428 None:intergenic 35.0%
AATTCATGGATAGGAAACAC+AGG - chr4.3:72841043-72841062 MS.gene48921:CDS 35.0%
AGGTTCATCCACTTCAAATA+GGG - chr4.3:72840943-72840962 MS.gene48921:CDS 35.0%
ATTTCATTGCCGGTAATTCA+TGG - chr4.3:72841029-72841048 MS.gene48921:CDS 35.0%
CAACAACCAAGTTACAAAGA+TGG - chr4.3:72838421-72838440 MS.gene48921:CDS 35.0%
CACAATATGAATCCCAAACA+TGG - chr4.3:72838257-72838276 MS.gene48921:CDS 35.0%
CACATGTATTGTCTTACGTT+TGG - chr4.3:72839864-72839883 MS.gene48921:intron 35.0%
CATAATTCCACATCCAATGT+AGG - chr4.3:72840087-72840106 MS.gene48921:intron 35.0%
CATCTTTGTAACTTGGTTGT+TGG + chr4.3:72838423-72838442 None:intergenic 35.0%
GGAAATCCATCTTTGTAACT+TGG + chr4.3:72838430-72838449 None:intergenic 35.0%
GTTTCCTATCCATGAATTAC+CGG + chr4.3:72841041-72841060 None:intergenic 35.0%
TACATAGCTTCTCATTCTAG+AGG + chr4.3:72839367-72839386 None:intergenic 35.0%
TATGTGATATATGTGAAGGC+TGG - chr4.3:72838595-72838614 MS.gene48921:CDS 35.0%
TCAAATAGGGTTACATGGAA+AGG - chr4.3:72840956-72840975 MS.gene48921:CDS 35.0%
TGCTTAGAAAGTTAACTAGC+CGG - chr4.3:72838963-72838982 MS.gene48921:intron 35.0%
TTGTAACTTGGTTGTTGGTA+AGG + chr4.3:72838418-72838437 None:intergenic 35.0%
! ACAAAGATGGATTTCCAACA+TGG - chr4.3:72838434-72838453 MS.gene48921:CDS 35.0%
! ACAATTTTGCAAAACTACCG+CGG + chr4.3:72840630-72840649 None:intergenic 35.0%
! ACTTTTGCTACCAGTGATTA+TGG + chr4.3:72838689-72838708 None:intergenic 35.0%
! ATCTAGGATTGATTCTAGAG+GGG + chr4.3:72839977-72839996 None:intergenic 35.0%
! ATGGGATGGTAATTTTGCTT+TGG - chr4.3:72840883-72840902 MS.gene48921:CDS 35.0%
! CAAAGATGGATTTCCAACAT+GGG - chr4.3:72838435-72838454 MS.gene48921:CDS 35.0%
! TTTCTTTTCATGGTCAAGAC+AGG + chr4.3:72838864-72838883 None:intergenic 35.0%
! TTTGGATTGGAATCTGCAAT+TGG - chr4.3:72840245-72840264 MS.gene48921:intron 35.0%
!! GAATATGCTAATACTGGTTC+AGG - chr4.3:72840923-72840942 MS.gene48921:CDS 35.0%
!!! TGTTTTTGGGCTCAATTATG+TGG + chr4.3:72839728-72839747 None:intergenic 35.0%
!!! TTTTTTTTTGCAGCTGCTAC+TGG - chr4.3:72840395-72840414 MS.gene48921:intron 35.0%
ACTGATCCAAACCAAAACAC+TGG - chr4.3:72840707-72840726 MS.gene48921:CDS 40.0%
ACTTCTTCAAACTTCTTCCG+CGG - chr4.3:72838477-72838496 MS.gene48921:CDS 40.0%
AGCAAAGAATGTCATGTTGG+TGG - chr4.3:72838645-72838664 MS.gene48921:CDS 40.0%
AGCTCTGTTAAATCGTACCT+TGG - chr4.3:72840779-72840798 MS.gene48921:CDS 40.0%
AGCTGTACTAAGCCATGTTT+GGG + chr4.3:72838272-72838291 None:intergenic 40.0%
AGGGTTTAAATCCGATTGAG+CGG + chr4.3:72840759-72840778 None:intergenic 40.0%
AGGTACGATTTAACAGAGCT+AGG + chr4.3:72840779-72840798 None:intergenic 40.0%
ATCACATTCAGAAACACAGC+TGG - chr4.3:72840440-72840459 MS.gene48921:intron 40.0%
ATGAAATTCCCAACGGTGAA+AGG + chr4.3:72841016-72841035 None:intergenic 40.0%
ATTGCCGGTAATTCATGGAT+AGG - chr4.3:72841034-72841053 MS.gene48921:CDS 40.0%
CAGGTTCATCCACTTCAAAT+AGG - chr4.3:72840942-72840961 MS.gene48921:CDS 40.0%
CCACTTCAAATAGGGTTACA+TGG - chr4.3:72840951-72840970 MS.gene48921:CDS 40.0%
CCATGTAACCCTATTTGAAG+TGG + chr4.3:72840954-72840973 None:intergenic 40.0%
CTGTTAAATCGTACCTTGGT+AGG - chr4.3:72840783-72840802 MS.gene48921:CDS 40.0%
GAGGAAAATACACATGCACT+TGG - chr4.3:72838130-72838149 MS.gene48921:CDS 40.0%
GCAAAGAATGTCATGTTGGT+GGG - chr4.3:72838646-72838665 MS.gene48921:CDS 40.0%
GGTACGATTTAACAGAGCTA+GGG + chr4.3:72840778-72840797 None:intergenic 40.0%
GTATTGTCTTACGTTTGGCT+AGG - chr4.3:72839869-72839888 MS.gene48921:intron 40.0%
TAACACCTTGTCACTCAACA+AGG - chr4.3:72838390-72838409 MS.gene48921:CDS 40.0%
TGAAGAAGTTTCCTATCACC+AGG + chr4.3:72838466-72838485 None:intergenic 40.0%
TGTATAACAACGGTCCTTCT+CGG - chr4.3:72839383-72839402 MS.gene48921:intron 40.0%
! ACACGTCATGCATTTTGGAT+TGG - chr4.3:72840232-72840251 MS.gene48921:intron 40.0%
! AGACATAGTTTGTGACACCA+AGG + chr4.3:72838333-72838352 None:intergenic 40.0%
! AGTACACACGTCATGCATTT+TGG - chr4.3:72840227-72840246 MS.gene48921:intron 40.0%
! ATCACTGGTAGCAAAAGTGT+TGG - chr4.3:72838691-72838710 MS.gene48921:CDS 40.0%
! GGGACAGTTGATTTCATCTT+TGG - chr4.3:72840599-72840618 MS.gene48921:CDS 40.0%
! GTAACTGTGATGGTTTTTGC+AGG + chr4.3:72840674-72840693 None:intergenic 40.0%
! GTGAAGCACTAGTAAGAACA+TGG + chr4.3:72840985-72841004 None:intergenic 40.0%
! TTTCCAACATGGGTTAAACC+TGG - chr4.3:72838445-72838464 MS.gene48921:CDS 40.0%
!! GTTTTATTAACCCTGCTGGT+TGG - chr4.3:72840855-72840874 MS.gene48921:CDS 40.0%
!!! GATGGTTTTATTAACCCTGC+TGG - chr4.3:72840851-72840870 MS.gene48921:CDS 40.0%
AAGCTTCACCTTTCACCGTT+GGG - chr4.3:72841005-72841024 MS.gene48921:CDS 45.0%
AATGTCGTCGTTGCTAAGGA+TGG - chr4.3:72838511-72838530 MS.gene48921:CDS 45.0%
AATTACTTGTGCTCCACGCT+AGG - chr4.3:72840357-72840376 MS.gene48921:intron 45.0%
ACATACCTTCCACTACCACT+TGG + chr4.3:72838580-72838599 None:intergenic 45.0%
ACCGGCAATGAAATTCCCAA+CGG + chr4.3:72841023-72841042 None:intergenic 45.0%
ACCGTTGGGAATTTCATTGC+CGG - chr4.3:72841019-72841038 MS.gene48921:CDS 45.0%
ACTTACCAACGGTTGCTGAA+CGG + chr4.3:72838735-72838754 None:intergenic 45.0%
AGGAACCTTGTTGAGTGACA+AGG + chr4.3:72838398-72838417 None:intergenic 45.0%
AGTTGGTCCTACATTGGATG+TGG + chr4.3:72840097-72840116 None:intergenic 45.0%
CAAGCTGTTGCATTTCGTTC+CGG - chr4.3:72840476-72840495 MS.gene48921:CDS 45.0%
CCCACAAGAAAAAGCTGCTT+GGG - chr4.3:72838168-72838187 MS.gene48921:CDS 45.0%
GAGCTGTACTAAGCCATGTT+TGG + chr4.3:72838273-72838292 None:intergenic 45.0%
TCACCAGGTTTAACCCATGT+TGG + chr4.3:72838451-72838470 None:intergenic 45.0%
TCTTACGTTTGGCTAGGACA+AGG - chr4.3:72839875-72839894 MS.gene48921:intron 45.0%
! AATGTCATGTTGGTGGGTGA+TGG - chr4.3:72838652-72838671 MS.gene48921:CDS 45.0%
! ACTGTGATGGTTTTTGCAGG+CGG + chr4.3:72840671-72840690 None:intergenic 45.0%
! ATGTTGGTGGGTGATGGTAT+TGG - chr4.3:72838658-72838677 MS.gene48921:CDS 45.0%
! CATGGGTTAAACCTGGTGAT+AGG - chr4.3:72838452-72838471 MS.gene48921:CDS 45.0%
! GATTCTAGAGGGGAAAGATG+TGG + chr4.3:72839967-72839986 None:intergenic 45.0%
! TTTCATCTTTGGAAACGCCG+CGG - chr4.3:72840610-72840629 MS.gene48921:CDS 45.0%
!! ACTGGTAGCAAAAGTGTTGG+AGG - chr4.3:72838694-72838713 MS.gene48921:CDS 45.0%
!! CAGCTTTTTCTTGTGGGCTA+CGG + chr4.3:72838165-72838184 None:intergenic 45.0%
!! CCCAAGCAGCTTTTTCTTGT+GGG + chr4.3:72838171-72838190 None:intergenic 45.0%
!! TTCTAGGGTTTCCGCTCAAT+CGG - chr4.3:72840745-72840764 MS.gene48921:CDS 45.0%
AAAGACCGATAGATCGGAGC+CGG + chr4.3:72840498-72840517 None:intergenic 50.0%
ACTTTCCGTTCAGCAACCGT+TGG - chr4.3:72838727-72838746 MS.gene48921:CDS 50.0%
CAAGCTTCACCTTTCACCGT+TGG - chr4.3:72841004-72841023 MS.gene48921:CDS 50.0%
CGTCGTTGCTAAGGATGGTT+CGG - chr4.3:72838516-72838535 MS.gene48921:CDS 50.0%
GCCCACAAGAAAAAGCTGCT+TGG - chr4.3:72838167-72838186 MS.gene48921:CDS 50.0%
GGAGAGAGCAAAAAAGGGTG+AGG - chr4.3:72838111-72838130 MS.gene48921:CDS 50.0%
GGCTAATGTCGTCGTTGCTA+AGG - chr4.3:72838507-72838526 MS.gene48921:CDS 50.0%
GGTTGCTGAACGGAAAGTAG+TGG + chr4.3:72838725-72838744 None:intergenic 50.0%
GTCGTTGCTAAGGATGGTTC+GGG - chr4.3:72838517-72838536 MS.gene48921:CDS 50.0%
TGGATCAGTTCTTCCTTGCG+CGG + chr4.3:72840696-72840715 None:intergenic 50.0%
TTTGCAGCTGCTACTGGTGA+TGG - chr4.3:72840401-72840420 MS.gene48921:intron 50.0%
! ATTTTTGCCACGGCCTACCA+AGG + chr4.3:72840799-72840818 None:intergenic 50.0%
! CTGTGATGGTTTTTGCAGGC+GGG + chr4.3:72840670-72840689 None:intergenic 50.0%
!! GCCCAAGCAGCTTTTTCTTG+TGG + chr4.3:72838172-72838191 None:intergenic 50.0%
!! GGTAGCAAAAGTGTTGGAGG+TGG - chr4.3:72838697-72838716 MS.gene48921:CDS 50.0%
AACCTTAACACCGGCCGAGA+AGG + chr4.3:72839400-72839419 None:intergenic 55.0%
AACTTCTTCCGCGGCTTCGA+AGG - chr4.3:72838486-72838505 MS.gene48921:CDS 55.0%
AATCGTACCTTGGTAGGCCG+TGG - chr4.3:72840789-72840808 MS.gene48921:CDS 55.0%
ACCATCACAGTTACCGCGCA+AGG - chr4.3:72840680-72840699 MS.gene48921:CDS 55.0%
CCCATGGTAACCAACCAGCA+GGG + chr4.3:72840868-72840887 None:intergenic 55.0%
GATGCTGCACCAAGTGGTAG+TGG - chr4.3:72838568-72838587 MS.gene48921:CDS 55.0%
TAACAACGGTCCTTCTCGGC+CGG - chr4.3:72839387-72839406 MS.gene48921:intron 55.0%
TCCCATGGTAACCAACCAGC+AGG + chr4.3:72840869-72840888 None:intergenic 55.0%
TCCTTGCGCGGTAACTGTGA+TGG + chr4.3:72840684-72840703 None:intergenic 55.0%
TCGTTCCGGCTCCGATCTAT+CGG - chr4.3:72840490-72840509 MS.gene48921:CDS 55.0%
! ACCCTGCTGGTTGGTTACCA+TGG - chr4.3:72840864-72840883 MS.gene48921:CDS 55.0%
! CCCTGCTGGTTGGTTACCAT+GGG - chr4.3:72840865-72840884 MS.gene48921:CDS 55.0%
! GCTGGTTGGTTACCATGGGA+TGG - chr4.3:72840869-72840888 MS.gene48921:CDS 55.0%
! TCTTGTGGGCTACGGCACTT+TGG + chr4.3:72838157-72838176 None:intergenic 55.0%
! TGGTTCGGGCAAGTACACGA+CGG - chr4.3:72838531-72838550 MS.gene48921:CDS 55.0%
!! CTGCACCAAGTGGTAGTGGA+AGG - chr4.3:72838572-72838591 MS.gene48921:CDS 55.0%
!! GACATTAGCCTTCGAAGCCG+CGG + chr4.3:72838497-72838516 None:intergenic 55.0%
GGGCAAGTACACGACGGTGA+AGG - chr4.3:72838537-72838556 MS.gene48921:CDS 60.0%
! ACAGGCGTGCCATTCACCTC+TGG - chr4.3:72841061-72841080 MS.gene48921:CDS 60.0%
! GTCCTTCTCGGCCGGTGTTA+AGG - chr4.3:72839395-72839414 MS.gene48921:intron 60.0%
!! ACTTGGTGCAGCATCCGTCG+CGG + chr4.3:72838563-72838582 None:intergenic 60.0%
GCGACGGATGCTGCACCAAG+TGG - chr4.3:72838562-72838581 MS.gene48921:CDS 65.0%
CACGACGGTGAAGGCCGCGA+CGG - chr4.3:72838546-72838565 MS.gene48921:CDS 70.0%


Chromosome Type Strat End Strand Name
chr4.3 gene 72838109 72841090 72838109 ID=MS.gene48921
chr4.3 mRNA 72838109 72841090 72838109 ID=MS.gene48921.t1;Parent=MS.gene48921
chr4.3 exon 72840451 72841090 72840451 ID=MS.gene48921.t1.exon1;Parent=MS.gene48921.t1
chr4.3 CDS 72840451 72841090 72840451 ID=cds.MS.gene48921.t1;Parent=MS.gene48921.t1
chr4.3 exon 72838109 72838791 72838109 ID=MS.gene48921.t1.exon2;Parent=MS.gene48921.t1
chr4.3 CDS 72838109 72838791 72838109 ID=cds.MS.gene48921.t1;Parent=MS.gene48921.t1
Gene Sequence

>MS.gene48921

ATGGAGAGAGCAAAAAAGGGTGAGGAAAATACACATGCACTTGGTCCAAAGTGCCGTAGCCCACAAGAAAAAGCTGCTTGGGCAGATTGTCTTGAACTTTATGATTTCACTGTCCAAAAGCTTAGCCAAACAAAATACACAAAGTGCACACAATATGAATCCCAAACATGGCTTAGTACAGCTCTCACAAATCTTGAAACATGCAAAAATGGATTTTATGACCTTGGTGTCACAAACTATGTCTTACCTTTGTTGTCTAATAATGTAACAAAGTTGTTAAGTAACACCTTGTCACTCAACAAGGTTCCTTACCAACAACCAAGTTACAAAGATGGATTTCCAACATGGGTTAAACCTGGTGATAGGAAACTTCTTCAAACTTCTTCCGCGGCTTCGAAGGCTAATGTCGTCGTTGCTAAGGATGGTTCGGGCAAGTACACGACGGTGAAGGCCGCGACGGATGCTGCACCAAGTGGTAGTGGAAGGTATGTGATATATGTGAAGGCTGGTGTGTATAATGAACAAGTTGAGATTAAAGCAAAGAATGTCATGTTGGTGGGTGATGGTATTGGTAAAACCATAATCACTGGTAGCAAAAGTGTTGGAGGTGGCACCACTACTTTCCGTTCAGCAACCGTTGGTAAGTACTTCTAGTAACAATTTATTTTACCGTACAGCTTTTTTTATCCCTTAAAGAAAAGTTCACAAATAAGACTATCATTATGAATTATTACCTTATTAAAAAGTTCTTACCTGTCTTGACCATGAAAAGAAAAGTTCATTCATTTTATCAACCAATAACTAAATCATAAACTAGTCACTTTCCTTTATTGAAAAATCTTATGCAAATTTCTTGCTTAGAAAGTTAACTAGCCGGTTTAATTCAAAGCTATAAAATAATACATATGCTTTTTTCTACTCATGAATAAATATGCGATATGTTTCAACCATTAATTTAAATATTTATTTATTTATTTATATACATGAAAATTACTTATGAATGATAGGTAAACGGGGTATACTTATTTTATTTCCATCTAGGGTTAAATTAATTTTTAATATTTACAAAAAAAAGTGTCGGTGATTATCTGATCTAACCTTTAAGTTATTTGTTATATTTATACTATTATAATTTTATTATAATTTCATAATCAATAAAAATGAATATACATACTTGTTACTATTATAATCTACATATATTTTCGTTTTATGAAAAATAAATAAATAATTATATCAGTAGGGGTATAGAAAATAACCTCTAGAATGAGAAGCTATGTATAACAACGGTCCTTCTCGGCCGGTGTTAAGGTTGCATATCTTATGGAACTTGAATAATATTCGTATTGACTTTAGATTTGTGTGGTATATGGCAAATTATCAATTACTCACAGACAAATGAGTCCTTGAGTAAGAAAGTAGATAATAGACCAAAGGATATAAATAATTATTCAAAATGATACATTATGCCATTGTGATTACTTTAAAATCTCTTTACGGTCCTTCTATGATATATTATCTTAATATTAATTATATAAAAGCAAGCAGCAAAGTTGAATTATGTCCCTTAATTTAATGATTAAGAAATTTATTAGACCACAATAATTGATTTAATTGAACCACATAATTGAGCCCAAAAACATAATGAATTGAATATCGTGGATTGAAATTTACAATCCAATAAAGTTTTAGACTATGATTTGAATCATTTACATGGATGATTGAATTATTATTATGTAGCTTTCAAAAATGAAGGAGCACATGTATTGTCTTACGTTTGGCTAGGACAAGGATCAAAATAAGATTTTTAGTAGTCAAATCTTTAGAGGAAGACGTGCCGACTATAACATATAGAAAGCCACATCTTTCCCCTCTAGAATCAATCCTAGATTAATATTTTTCCTTAATGAACCGCATATATAGTTTCCTATATGTGGTAACACTTTAACTAATTTCTTAATTGGTAGTTCAAACTTGTTTTCATAATTCCACATCCAATGTAGGACCAACTTTATTATAGTCTTATTCAATTGAAGATCCTTTAATTAAAAGAAGAATGTGGGGCTTTAGAACCATGTATACCATTACTTAATAATCAGTTGAACTATATAAATTAATCATAGTACACACGTCATGCATTTTGGATTGGAATCTGCAATTGGTTAGAATGTCACTTTGAGATTTGAAAAATGTATACATAAACTAATAGTCAATGCAACTGTTTTTTTATTAGGTTTTTTTTTTTAAATTCAATTACTTGTGCTCCACGCTAGGTTTTTTAATACTTTTTTTTTTTTTGCAGCTGCTACTGGTGATGGATTTATTGCTCAAGACATCACATTCAGAAACACAGCTGGAGCTGCAAATCATCAAGCTGTTGCATTTCGTTCCGGCTCCGATCTATCGGTCTTTTACAAATGTAGCTTTGAAGGTTATCAAGACACACTTTATGTTCACTCAGAGAGACAATTTTACAGAGAATGTAACATCTATGGGACAGTTGATTTCATCTTTGGAAACGCCGCGGTAGTTTTGCAAAATTGTAACATTTTTGCAAGAAACCCGCCTGCAAAAACCATCACAGTTACCGCGCAAGGAAGAACTGATCCAAACCAAAACACTGGAATAATAATTCATAATTCTAGGGTTTCCGCTCAATCGGATTTAAACCCTAGCTCTGTTAAATCGTACCTTGGTAGGCCGTGGCAAAAATATTCAAGAACAGTTTTCATGAAAACTGTTATTGATGGTTTTATTAACCCTGCTGGTTGGTTACCATGGGATGGTAATTTTGCTTTGGATACTTTATATTATGCTGAATATGCTAATACTGGTTCAGGTTCATCCACTTCAAATAGGGTTACATGGAAAGGTTACCATGTTCTTACTAGTGCTTCACAAGCTTCACCTTTCACCGTTGGGAATTTCATTGCCGGTAATTCATGGATAGGAAACACAGGCGTGCCATTCACCTCTGGACTTTAA
Protein sequence

>MS.gene48921.t1

MERAKKGEENTHALGPKCRSPQEKAAWADCLELYDFTVQKLSQTKYTKCTQYESQTWLSTALTNLETCKNGFYDLGVTNYVLPLLSNNVTKLLSNTLSLNKVPYQQPSYKDGFPTWVKPGDRKLLQTSSAASKANVVVAKDGSGKYTTVKAATDAAPSGSGRYVIYVKAGVYNEQVEIKAKNVMLVGDGIGKTIITGSKSVGGGTTTFRSATVAATGDGFIAQDITFRNTAGAANHQAVAFRSGSDLSVFYKCSFEGYQDTLYVHSERQFYRECNIYGTVDFIFGNAAVVLQNCNIFARNPPAKTITVTAQGRTDPNQNTGIIIHNSRVSAQSDLNPSSVKSYLGRPWQKYSRTVFMKTVIDGFINPAGWLPWDGNFALDTLYYAEYANTGSGSSTSNRVTWKGYHVLTSASQASPFTVGNFIAGNSWIGNTGVPFTSGL