AlfalfaGEDB | MS.gene058176

M. sativa cultivar XinJiangDaYe / MS.gene058176

KEGG: -
GO: GO:0003674 GO:0004857 GO:0004864 GO:0005488 GO:0006950 GO:0006952 GO:0007165 GO:0008150 GO:0008289 GO:0009738 GO:0009755 GO:0009987 GO:0010427 GO:0019208 GO:0019212 GO:0019840 GO:0019888 GO:0030234 GO:0031406 GO:0033293 GO:0036094 GO:0038023 GO:0042562 GO:0043167 GO:0043168 GO:0043177 GO:0043178 GO:0050789 GO:0050794 GO:0050896 GO:0060089 GO:0065007 GO:0098772
PFAM: PF00407.22

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene058176.t1	SNQ45214.1	98.1	156	3	0	2	157	3	158	6.60E-80	306.6

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene058176.t1	P14710	87.8	156	19	0	2	157	3	158	2.1e-74	279.6

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene058176.t1	A0A238U3J0	98.1	156	3	0	2	157	3	158	4.8e-80	306.6

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene050380	MS.gene058176	0.814591	1.43E-51	-1.69E-46
MS.gene055124	MS.gene058176	0.811005	8.69E-51	-1.69E-46
MS.gene056313	MS.gene058176	0.832199	1.08E-55	-1.69E-46
MS.gene058093	MS.gene058176	0.832891	7.30E-56	-1.69E-46
MS.gene058108	MS.gene058176	0.812386	4.35E-51	-1.69E-46
MS.gene058171	MS.gene058176	0.80832	3.28E-50	-1.69E-46
MS.gene058173	MS.gene058176	0.813378	2.64E-51	-1.69E-46
MS.gene058175	MS.gene058176	0.809689	1.67E-50	-1.69E-46
MS.gene058176	MS.gene058179	0.863203	2.94E-64	-1.69E-46
MS.gene058176	MS.gene058180	0.869087	4.03E-66	-1.69E-46
MS.gene058176	MS.gene059037	0.844858	5.83E-59	-1.69E-46
MS.gene058176	MS.gene059038	0.819687	1.02E-52	-1.69E-46
MS.gene058176	MS.gene059044	0.944093	3.67E-103	-1.69E-46
MS.gene058176	MS.gene059045	0.802235	6.18E-49	-1.69E-46
MS.gene058176	MS.gene068296	0.851445	8.84E-61	-1.69E-46
MS.gene058176	MS.gene068297	0.897212	1.75E-76	-1.69E-46
MS.gene058176	MS.gene071838	0.847621	1.03E-59	-1.69E-46
MS.gene058176	MS.gene071842	0.888453	5.82E-73	-1.69E-46
MS.gene058176	MS.gene071843	0.80938	1.95E-50	-1.69E-46
MS.gene058176	MS.gene072806	0.805716	1.17E-49	-1.69E-46
MS.gene058176	MS.gene21757	0.809822	1.56E-50	-1.69E-46
MS.gene058176	MS.gene21758	0.810696	1.01E-50	-1.69E-46
MS.gene058176	MS.gene21772	0.812413	4.29E-51	-1.69E-46
MS.gene058176	MS.gene21867	0.804499	2.10E-49	-1.69E-46
MS.gene058176	MS.gene24974	0.899927	1.22E-77	-1.69E-46
MS.gene058176	MS.gene26403	0.81161	6.42E-51	-1.69E-46
MS.gene058176	MS.gene26860	0.810225	1.28E-50	-1.69E-46
MS.gene058176	MS.gene27550	0.801428	9.05E-49	-1.69E-46
MS.gene058176	MS.gene27779	0.816626	5.02E-52	-1.69E-46
MS.gene058176	MS.gene33429	0.807044	6.12E-50	-1.69E-46
MS.gene058176	MS.gene33683	0.812262	4.63E-51	-1.69E-46
MS.gene058176	MS.gene38173	0.81573	7.96E-52	-1.69E-46
MS.gene058176	MS.gene39533	0.830832	2.35E-55	-1.69E-46
MS.gene058176	MS.gene44720	0.806009	1.01E-49	-1.69E-46
MS.gene058176	MS.gene46024	0.84169	4.08E-58	-1.69E-46
MS.gene058176	MS.gene46025	0.859928	2.95E-63	-1.69E-46
MS.gene058176	MS.gene48183	0.811198	7.89E-51	-1.69E-46
MS.gene058176	MS.gene48921	0.805069	1.59E-49	-1.69E-46
MS.gene058176	MS.gene59006	0.823259	1.52E-53	-1.69E-46
MS.gene058176	MS.gene59394	0.862391	5.24E-64	-1.69E-46
MS.gene058176	MS.gene59395	0.867779	1.06E-65	-1.69E-46
MS.gene058176	MS.gene59396	0.847811	9.13E-60	-1.69E-46
MS.gene058176	MS.gene62114	0.806781	6.96E-50	-1.69E-46
MS.gene058176	MS.gene62120	0.804156	2.47E-49	-1.69E-46
MS.gene058176	MS.gene64686	0.83127	1.84E-55	-1.69E-46
MS.gene058176	MS.gene64687	0.821198	4.59E-53	-1.69E-46
MS.gene058176	MS.gene70420	0.844207	8.72E-59	-1.69E-46
MS.gene058176	MS.gene71525	0.835029	2.13E-56	-1.69E-46
MS.gene058176	MS.gene75392	0.813807	2.12E-51	-1.69E-46
MS.gene058176	MS.gene75396	0.832283	1.03E-55	-1.69E-46
MS.gene058176	MS.gene77471	0.829483	5.02E-55	-1.69E-46
MS.gene058176	MS.gene84568	0.855636	5.53E-62	-1.69E-46
MS.gene058176	MS.gene91977	0.818037	2.42E-52	-1.69E-46
MS.gene058176	MS.gene94790	0.84223	2.94E-58	-1.69E-46
MS.gene058176	MS.gene96173	0.806865	6.68E-50	-1.69E-46
MS.gene058176	MS.gene97500	0.83887	2.22E-57	-1.69E-46

PPI:

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene058176.t1	MTR_2g035150	96.795	156	5	0	2	157	3	158	4.74e-106	300
MS.gene058176.t1	MTR_2g035130	89.032	155	17	0	2	156	3	157	1.18e-97	278
MS.gene058176.t1	MTR_2g035120	87.179	156	20	0	2	157	3	158	8.89e-96	273
MS.gene058176.t1	MTR_2g035100	85.161	155	23	0	2	156	3	157	1.22e-92	266
MS.gene058176.t1	MTR_2g035105	83.871	155	25	0	2	156	3	157	2.52e-91	262
MS.gene058176.t1	MTR_6g033450	74.359	156	40	0	2	157	3	158	5.38e-81	236
MS.gene058176.t1	MTR_4g120970	74.359	156	40	0	2	157	3	158	5.38e-81	236
MS.gene058176.t1	MTR_2g035320	65.806	155	53	0	2	156	3	157	6.54e-68	203
MS.gene058176.t1	MTR_2g035190	63.871	155	56	0	2	156	3	157	2.82e-65	196
MS.gene058176.t1	MTR_2g035210	62.581	155	58	0	2	156	3	157	7.04e-64	193
MS.gene058176.t1	MTR_2g035220	62.987	154	57	0	3	156	4	157	2.29e-62	189
MS.gene058176.t1	MTR_2g035170	58.940	151	62	0	6	156	7	157	3.18e-60	184
MS.gene058176.t1	MTR_2g035320	60.606	99	39	0	58	156	24	122	6.02e-37	123
MS.gene058176.t1	MTR_2g435310	38.710	155	94	1	1	154	2	156	2.30e-36	123
MS.gene058176.t1	MTR_4g120950	44.375	160	82	5	2	157	3	159	3.67e-34	117
MS.gene058176.t1	MTR_4g120760	38.961	154	93	1	2	154	3	156	6.83e-34	117
MS.gene058176.t1	MTR_4g120760	38.961	154	93	1	2	154	3	156	7.87e-34	117
MS.gene058176.t1	MTR_4g120940	64.045	89	29	1	69	157	35	120	2.71e-32	111

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Find 39 sgRNAs with CRISPR-Local

Find 46 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AACAATTTCAACACTCTTGA+TGG	0.245294	2.1:+57689661	None:intergenic
TGATGGCATCAATAACCTTT+GGG	0.301288	2.1:+57689678	None:intergenic
TTGATGGCATCAATAACCTT+TGG	0.345179	2.1:+57689677	None:intergenic
GGCTATAGAGGGTTACTGTT+TGG	0.394448	2.1:-57689176	MS.gene058176:CDS
CTTAGTAGATGATGCTAACT+TGG	0.397103	2.1:-57689395	MS.gene058176:CDS
GCTTACAACTACAGCATTGT+TGG	0.405129	2.1:-57689373	MS.gene058176:CDS
TTTGCAATGGATCCTCCATT+TGG	0.420662	2.1:+57689289	None:intergenic
TATATGCTTTAGTTGTAGTC+AGG	0.421813	2.1:+57689148	None:intergenic
TACAGCATTGTTGGAGGTGT+TGG	0.427537	2.1:-57689364	MS.gene058176:CDS
GAGATCTTCTCTATTGTGTC+TGG	0.431898	2.1:+57689337	None:intergenic
CATCAATAACCTTTGGGGTA+AGG	0.433870	2.1:+57689684	None:intergenic
AAGAGTGTTGAAATTGTTGA+AGG	0.434561	2.1:-57689656	MS.gene058176:CDS
CACTTTGTGCAAGACATGCT+TGG	0.461268	2.1:+57689420	None:intergenic
AGAGCTTTGTAAAGTGTAGC+AGG	0.462210	2.1:+57689725	None:intergenic
AAATTGTCTGCAGGTCCAAA+TGG	0.485800	2.1:-57689304	MS.gene058176:CDS
ACTTTACAAAGCTCTAGTTA+AGG	0.493500	2.1:-57689717	MS.gene058176:CDS
AATAGAGAAGATCTCATTTG+AGG	0.494265	2.1:-57689329	MS.gene058176:CDS
AGCAGGGGCTACAGTGGAGG+TGG	0.494653	2.1:+57689742	None:intergenic
CTCTTGAGTTCCTCTTCACT+AGG	0.501934	2.1:+57689229	None:intergenic
AATGTGAAATACTTCACAAA+AGG	0.503374	2.1:-57689262	MS.gene058176:CDS
TTTCACATTAAGCTTTGCAA+TGG	0.512266	2.1:+57689276	None:intergenic
GTTGAAGGAAACGGTGGTGC+CGG	0.528042	2.1:-57689641	MS.gene058176:CDS
ACTCAAGAGTGGCAAAGCTA+AGG	0.529920	2.1:-57689215	MS.gene058176:CDS
CTCAAGAGTGGCAAAGCTAA+GGG	0.532020	2.1:-57689214	MS.gene058176:CDS
TTTGAGGCTAAATTGTCTGC+AGG	0.535432	2.1:-57689313	MS.gene058176:CDS
GTTGAAATTGTTGAAGGAAA+CGG	0.548798	2.1:-57689650	MS.gene058176:CDS
TACAACTACAGCATTGTTGG+AGG	0.566488	2.1:-57689370	MS.gene058176:CDS
GATGGCATCAATAACCTTTG+GGG	0.578696	2.1:+57689679	None:intergenic
CAAGCATGTCTTGCACAAAG+TGG	0.581918	2.1:-57689419	MS.gene058176:CDS
AAGTGTAGCAGGGGCTACAG+TGG	0.591266	2.1:+57689736	None:intergenic
AGTGGCAAAGCTAAGGGTGA+TGG	0.593000	2.1:-57689208	MS.gene058176:CDS
TTCTGACAACCTTACCCCAA+AGG	0.596498	2.1:-57689693	MS.gene058176:CDS
GAGCTTTGTAAAGTGTAGCA+GGG	0.601987	2.1:+57689726	None:intergenic
AGATGTTACTCCTAGTGAAG+AGG	0.628737	2.1:-57689239	MS.gene058176:CDS
TGTAGCAGGGGCTACAGTGG+AGG	0.638855	2.1:+57689739	None:intergenic
TTGTCTGCAGGTCCAAATGG+AGG	0.646240	2.1:-57689301	MS.gene058176:CDS
AGCTTTGTAAAGTGTAGCAG+GGG	0.650529	2.1:+57689727	None:intergenic
AGTGAAGAGGAACTCAAGAG+TGG	0.684104	2.1:-57689226	MS.gene058176:CDS
GAAATTGTTGAAGGAAACGG+TGG	0.749751	2.1:-57689647	MS.gene058176:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!	AATGTGAAATACTTCACAAA+AGG	-	chr2.1:57689661-57689680	MS.gene058176:CDS	25.0%
	AAAAAACTCACATTCGTTGA+AGG	-	chr2.1:57689312-57689331	MS.gene058176:CDS	30.0%
	AACAATTTCAACACTCTTGA+TGG	+	chr2.1:57689265-57689284	None:intergenic	30.0%
	AATAGAGAAGATCTCATTTG+AGG	-	chr2.1:57689594-57689613	MS.gene058176:intron	30.0%
	GTTGAAATTGTTGAAGGAAA+CGG	-	chr2.1:57689273-57689292	MS.gene058176:CDS	30.0%
	TGATGTTTGTGATTGTGAAA+CGG	-	chr2.1:57689459-57689478	MS.gene058176:intron	30.0%
	TTTCACATTAAGCTTTGCAA+TGG	+	chr2.1:57689650-57689669	None:intergenic	30.0%
!	ACTTTACAAAGCTCTAGTTA+AGG	-	chr2.1:57689206-57689225	MS.gene058176:CDS	30.0%
!!	AAGAGTGTTGAAATTGTTGA+AGG	-	chr2.1:57689267-57689286	MS.gene058176:CDS	30.0%
!!!	TGTGAGTTTTTTGATAGTTC+CGG	+	chr2.1:57689304-57689323	None:intergenic	30.0%
	CTTAGTAGATGATGCTAACT+TGG	-	chr2.1:57689528-57689547	MS.gene058176:intron	35.0%
	TGATGGCATCAATAACCTTT+GGG	+	chr2.1:57689248-57689267	None:intergenic	35.0%
!	TTGATGGCATCAATAACCTT+TGG	+	chr2.1:57689249-57689268	None:intergenic	35.0%
	AAATTGTCTGCAGGTCCAAA+TGG	-	chr2.1:57689619-57689638	MS.gene058176:CDS	40.0%
	AACTCACATTCGTTGAAGGT+CGG	-	chr2.1:57689316-57689335	MS.gene058176:CDS	40.0%
	AGATGTTACTCCTAGTGAAG+AGG	-	chr2.1:57689684-57689703	MS.gene058176:CDS	40.0%
	GAAATTGTTGAAGGAAACGG+TGG	-	chr2.1:57689276-57689295	MS.gene058176:CDS	40.0%
	GAGATCTTCTCTATTGTGTC+TGG	+	chr2.1:57689589-57689608	None:intergenic	40.0%
	GATGGCATCAATAACCTTTG+GGG	+	chr2.1:57689247-57689266	None:intergenic	40.0%
	GCTTACAACTACAGCATTGT+TGG	-	chr2.1:57689550-57689569	MS.gene058176:intron	40.0%
	TACAACTACAGCATTGTTGG+AGG	-	chr2.1:57689553-57689572	MS.gene058176:intron	40.0%
	TTTGCAATGGATCCTCCATT+TGG	+	chr2.1:57689637-57689656	None:intergenic	40.0%
!	AGAGCTTTGTAAAGTGTAGC+AGG	+	chr2.1:57689201-57689220	None:intergenic	40.0%
!	AGCTTTGTAAAGTGTAGCAG+GGG	+	chr2.1:57689199-57689218	None:intergenic	40.0%
!	GAGCTTTGTAAAGTGTAGCA+GGG	+	chr2.1:57689200-57689219	None:intergenic	40.0%
!	TTTGAGGCTAAATTGTCTGC+AGG	-	chr2.1:57689610-57689629	MS.gene058176:intron	40.0%
!!	CATCAATAACCTTTGGGGTA+AGG	+	chr2.1:57689242-57689261	None:intergenic	40.0%
!!	GGTCTTTTCAAGGCTATAGA+GGG	-	chr2.1:57689736-57689755	MS.gene058176:CDS	40.0%
!!	TAAGGGTGATGGTCTTTTCA+AGG	-	chr2.1:57689726-57689745	MS.gene058176:CDS	40.0%
!!	TGGTCTTTTCAAGGCTATAG+AGG	-	chr2.1:57689735-57689754	MS.gene058176:CDS	40.0%
	ACTCAAGAGTGGCAAAGCTA+AGG	-	chr2.1:57689708-57689727	MS.gene058176:CDS	45.0%
	AGTGAAGAGGAACTCAAGAG+TGG	-	chr2.1:57689697-57689716	MS.gene058176:CDS	45.0%
	CAAGCATGTCTTGCACAAAG+TGG	-	chr2.1:57689504-57689523	MS.gene058176:intron	45.0%
	CACTTTGTGCAAGACATGCT+TGG	+	chr2.1:57689506-57689525	None:intergenic	45.0%
	CTCAAGAGTGGCAAAGCTAA+GGG	-	chr2.1:57689709-57689728	MS.gene058176:CDS	45.0%
	CTCTTGAGTTCCTCTTCACT+AGG	+	chr2.1:57689697-57689716	None:intergenic	45.0%
	GAAACGGATCAATGTGCAGA+TGG	-	chr2.1:57689475-57689494	MS.gene058176:intron	45.0%
	GGCTATAGAGGGTTACTGTT+TGG	-	chr2.1:57689747-57689766	MS.gene058176:CDS	45.0%
	TTCTGACAACCTTACCCCAA+AGG	-	chr2.1:57689230-57689249	MS.gene058176:CDS	45.0%
!	TACAGCATTGTTGGAGGTGT+TGG	-	chr2.1:57689559-57689578	MS.gene058176:intron	45.0%
	AGTGGCAAAGCTAAGGGTGA+TGG	-	chr2.1:57689715-57689734	MS.gene058176:CDS	50.0%
	TTGTCTGCAGGTCCAAATGG+AGG	-	chr2.1:57689622-57689641	MS.gene058176:CDS	50.0%
	AAGTGTAGCAGGGGCTACAG+TGG	+	chr2.1:57689190-57689209	None:intergenic	55.0%
	GTTGAAGGAAACGGTGGTGC+CGG	-	chr2.1:57689282-57689301	MS.gene058176:CDS	55.0%
	TGTAGCAGGGGCTACAGTGG+AGG	+	chr2.1:57689187-57689206	None:intergenic	60.0%
	AGCAGGGGCTACAGTGGAGG+TGG	+	chr2.1:57689184-57689203	None:intergenic	65.0%

Chromosome	Type	Strat	End	Strand	Name
chr2.1	gene	57689156	57689789	57689156	ID=MS.gene058176
chr2.1	mRNA	57689156	57689789	57689156	ID=MS.gene058176.t1;Parent=MS.gene058176
chr2.1	exon	57689612	57689789	57689612	ID=MS.gene058176.t1.exon1;Parent=MS.gene058176.t1
chr2.1	CDS	57689612	57689789	57689612	ID=cds.MS.gene058176.t1;Parent=MS.gene058176.t1
chr2.1	exon	57689156	57689451	57689156	ID=MS.gene058176.t1.exon2;Parent=MS.gene058176.t1
chr2.1	CDS	57689156	57689451	57689156	ID=cds.MS.gene058176.t1;Parent=MS.gene058176.t1

Gene Sequence

>MS.gene058176

ATGGTGTTCAATTTTGAGGATGAAACCACCTCCACTGTAGCCCCTGCTACACTTTACAAAGCTCTAGTTAAGGATTCTGACAACCTTACCCCAAAGGTTATTGATGCCATCAAGAGTGTTGAAATTGTTGAAGGAAACGGTGGTGCCGGAACTATCAAAAAACTCACATTCGTTGAAGGTCGGTATAAATATATTTATGTTATTTTTATAGTCAAATATTAATAATTAATTGTTACGTTAGTGTTTAAAAAAATTATGTTTTCTACATCGAGTCTAAGTTCATCGTCTTTGAAGTAATGCTGATGATGTTTGTGATTGTGAAACGGATCAATGTGCAGATGGTGAGACCAAGCATGTCTTGCACAAAGTGGACTTAGTAGATGATGCTAACTTGGCTTACAACTACAGCATTGTTGGAGGTGTTGGTCTTCCAGACACAATAGAGAAGATCTCATTTGAGGCTAAATTGTCTGCAGGTCCAAATGGAGGATCCATTGCAAAGCTTAATGTGAAATACTTCACAAAAGGAGATGTTACTCCTAGTGAAGAGGAACTCAAGAGTGGCAAAGCTAAGGGTGATGGTCTTTTCAAGGCTATAGAGGGTTACTGTTTGGCTAATCCTGACTACAACTAA

Protein sequence

>MS.gene058176.t1

MVFNFEDETTSTVAPATLYKALVKDSDNLTPKVIDAIKSVEIVEGNGGAGTIKKLTFVEDGETKHVLHKVDLVDDANLAYNYSIVGGVGLPDTIEKISFEAKLSAGPNGGSIAKLNVKYFTKGDVTPSEEELKSGKAKGDGLFKAIEGYCLANPDYN