Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene51240.t1 | XP_013452956.1 | 86.4 | 508 | 69 | 0 | 1 | 508 | 1 | 508 | 7.10E-115 | 424.5 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene51240.t1 | Q84VY2 | 49.0 | 51 | 26 | 0 | 456 | 506 | 463 | 513 | 2.1e-06 | 55.5 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene51240.t1 | A0A072UB28 | 86.4 | 508 | 69 | 0 | 1 | 508 | 1 | 508 | 5.1e-115 | 424.5 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049142 | MS.gene51240 | 0.827546 | 1.47E-54 | -1.69E-46 |
| MS.gene049159 | MS.gene51240 | 0.800065 | 1.72E-48 | -1.69E-46 |
| MS.gene049178 | MS.gene51240 | 0.800007 | 1.76E-48 | -1.69E-46 |
| MS.gene049341 | MS.gene51240 | 0.811756 | 5.97E-51 | -1.69E-46 |
| MS.gene049382 | MS.gene51240 | 0.804508 | 2.09E-49 | -1.69E-46 |
| MS.gene049501 | MS.gene51240 | 0.828937 | 6.81E-55 | -1.69E-46 |
| MS.gene049850 | MS.gene51240 | 0.801803 | 7.58E-49 | -1.69E-46 |
| MS.gene049887 | MS.gene51240 | 0.814593 | 1.42E-51 | -1.69E-46 |
| MS.gene049979 | MS.gene51240 | 0.810097 | 1.37E-50 | -1.69E-46 |
| MS.gene050020 | MS.gene51240 | 0.801347 | 9.40E-49 | -1.69E-46 |
| MS.gene050807 | MS.gene51240 | 0.830855 | 2.32E-55 | -1.69E-46 |
| MS.gene050815 | MS.gene51240 | 0.828701 | 7.77E-55 | -1.69E-46 |
| MS.gene050959 | MS.gene51240 | 0.821058 | 4.94E-53 | -1.69E-46 |
| MS.gene051102 | MS.gene51240 | 0.829929 | 3.91E-55 | -1.69E-46 |
| MS.gene051180 | MS.gene51240 | 0.824391 | 8.28E-54 | -1.69E-46 |
| MS.gene051247 | MS.gene51240 | 0.82585 | 3.74E-54 | -1.69E-46 |
| MS.gene051328 | MS.gene51240 | 0.823605 | 1.27E-53 | -1.69E-46 |
| MS.gene05157 | MS.gene51240 | 0.825137 | 5.52E-54 | -1.69E-46 |
| MS.gene051700 | MS.gene51240 | 0.801508 | 8.71E-49 | -1.69E-46 |
| MS.gene051719 | MS.gene51240 | 0.81372 | 2.22E-51 | -1.69E-46 |
| MS.gene051720 | MS.gene51240 | 0.81967 | 1.03E-52 | -1.69E-46 |
| MS.gene052020 | MS.gene51240 | 0.812996 | 3.20E-51 | -1.69E-46 |
| MS.gene052133 | MS.gene51240 | 0.82935 | 5.41E-55 | -1.69E-46 |
| MS.gene052397 | MS.gene51240 | 0.806623 | 7.52E-50 | -1.69E-46 |
| MS.gene052516 | MS.gene51240 | 0.816847 | 4.48E-52 | -1.69E-46 |
| MS.gene052613 | MS.gene51240 | 0.800979 | 1.12E-48 | -1.69E-46 |
| MS.gene052665 | MS.gene51240 | 0.856146 | 3.92E-62 | -1.69E-46 |
| MS.gene052732 | MS.gene51240 | 0.812729 | 3.66E-51 | -1.69E-46 |
| MS.gene052863 | MS.gene51240 | 0.818655 | 1.75E-52 | -1.69E-46 |
| MS.gene052896 | MS.gene51240 | 0.835022 | 2.14E-56 | -1.69E-46 |
| MS.gene053144 | MS.gene51240 | 0.806767 | 7.01E-50 | -1.69E-46 |
| MS.gene053437 | MS.gene51240 | 0.800723 | 1.26E-48 | -1.69E-46 |
| MS.gene053581 | MS.gene51240 | 0.817873 | 2.63E-52 | -1.69E-46 |
| MS.gene054472 | MS.gene51240 | 0.815176 | 1.06E-51 | -1.69E-46 |
| MS.gene054584 | MS.gene51240 | 0.808087 | 3.68E-50 | -1.69E-46 |
| MS.gene05462 | MS.gene51240 | 0.80327 | 3.78E-49 | -1.69E-46 |
| MS.gene054823 | MS.gene51240 | 0.815142 | 1.08E-51 | -1.69E-46 |
| MS.gene055465 | MS.gene51240 | 0.860854 | 1.54E-63 | -1.69E-46 |
| MS.gene055466 | MS.gene51240 | 0.859299 | 4.55E-63 | -1.69E-46 |
| MS.gene055468 | MS.gene51240 | 0.862606 | 4.50E-64 | -1.69E-46 |
| MS.gene055469 | MS.gene51240 | 0.849812 | 2.54E-60 | -1.69E-46 |
| MS.gene055512 | MS.gene51240 | 0.807095 | 5.97E-50 | -1.69E-46 |
| MS.gene055580 | MS.gene51240 | -0.826064 | 3.33E-54 | -1.69E-46 |
| MS.gene055608 | MS.gene51240 | 0.815516 | 8.88E-52 | -1.69E-46 |
| MS.gene055891 | MS.gene51240 | 0.800981 | 1.12E-48 | -1.69E-46 |
| MS.gene056043 | MS.gene51240 | -0.824759 | 6.78E-54 | -1.69E-46 |
| MS.gene056308 | MS.gene51240 | 0.807277 | 5.47E-50 | -1.69E-46 |
| MS.gene056432 | MS.gene51240 | 0.840156 | 1.03E-57 | -1.69E-46 |
| MS.gene056553 | MS.gene51240 | 0.807334 | 5.32E-50 | -1.69E-46 |
| MS.gene056760 | MS.gene51240 | 0.807722 | 4.40E-50 | -1.69E-46 |
| MS.gene057446 | MS.gene51240 | 0.808135 | 3.59E-50 | -1.69E-46 |
| MS.gene057915 | MS.gene51240 | 0.826007 | 3.44E-54 | -1.69E-46 |
| MS.gene058087 | MS.gene51240 | 0.808332 | 3.26E-50 | -1.69E-46 |
| MS.gene058168 | MS.gene51240 | 0.806613 | 7.56E-50 | -1.69E-46 |
| MS.gene058636 | MS.gene51240 | 0.82394 | 1.06E-53 | -1.69E-46 |
| MS.gene058646 | MS.gene51240 | 0.814161 | 1.77E-51 | -1.69E-46 |
| MS.gene058852 | MS.gene51240 | 0.832968 | 6.98E-56 | -1.69E-46 |
| MS.gene058906 | MS.gene51240 | 0.818507 | 1.89E-52 | -1.69E-46 |
| MS.gene059171 | MS.gene51240 | 0.815418 | 9.34E-52 | -1.69E-46 |
| MS.gene059713 | MS.gene51240 | 0.801576 | 8.44E-49 | -1.69E-46 |
| MS.gene059725 | MS.gene51240 | 0.832288 | 1.03E-55 | -1.69E-46 |
| MS.gene059842 | MS.gene51240 | 0.808756 | 2.65E-50 | -1.69E-46 |
| MS.gene059937 | MS.gene51240 | 0.830834 | 2.35E-55 | -1.69E-46 |
| MS.gene060429 | MS.gene51240 | 0.808216 | 3.45E-50 | -1.69E-46 |
| MS.gene060637 | MS.gene51240 | 0.820072 | 8.32E-53 | -1.69E-46 |
| MS.gene060803 | MS.gene51240 | 0.801889 | 7.28E-49 | -1.69E-46 |
| MS.gene061006 | MS.gene51240 | 0.802711 | 4.93E-49 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene51240.t1 | MTR_6g082800 | 93.504 | 508 | 33 | 0 | 1 | 508 | 1 | 508 | 0.0 | 927 |
| MS.gene51240.t1 | MTR_8g032900 | 45.857 | 519 | 222 | 12 | 1 | 500 | 1 | 479 | 6.08e-111 | 338 |
| MS.gene51240.t1 | MTR_8g032930 | 44.007 | 559 | 208 | 15 | 1 | 508 | 1 | 505 | 2.14e-107 | 329 |
| MS.gene51240.t1 | MTR_3g093770 | 43.659 | 552 | 206 | 10 | 23 | 497 | 2 | 525 | 3.16e-107 | 330 |
| MS.gene51240.t1 | MTR_3g093750 | 45.842 | 469 | 195 | 11 | 1 | 447 | 1 | 432 | 2.04e-95 | 299 |
| MS.gene51240.t1 | MTR_5g075490 | 31.670 | 521 | 314 | 10 | 2 | 508 | 112 | 604 | 8.03e-59 | 205 |
| MS.gene51240.t1 | MTR_3g093720 | 57.616 | 151 | 50 | 3 | 115 | 251 | 46 | 196 | 1.87e-34 | 131 |
| MS.gene51240.t1 | MTR_3g467610 | 26.848 | 514 | 280 | 10 | 20 | 508 | 94 | 536 | 2.29e-33 | 133 |
| MS.gene51240.t1 | MTR_7g022780 | 36.726 | 226 | 114 | 3 | 232 | 457 | 3 | 199 | 1.25e-24 | 102 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene51240.t1 | AT5G05180 | 34.783 | 299 | 156 | 5 | 236 | 506 | 115 | 402 | 7.46e-41 | 152 |
| MS.gene51240.t1 | AT5G05180 | 34.783 | 299 | 156 | 5 | 236 | 506 | 145 | 432 | 9.48e-41 | 152 |
| MS.gene51240.t1 | AT5G05180 | 34.114 | 299 | 134 | 5 | 236 | 506 | 145 | 408 | 6.61e-38 | 144 |
| MS.gene51240.t1 | AT5G58320 | 25.382 | 524 | 290 | 12 | 8 | 508 | 113 | 558 | 4.11e-21 | 97.1 |
| MS.gene51240.t1 | AT5G58320 | 25.382 | 524 | 290 | 12 | 8 | 508 | 113 | 558 | 4.11e-21 | 97.1 |
| MS.gene51240.t1 | AT2G30500 | 27.681 | 401 | 208 | 13 | 115 | 507 | 188 | 514 | 6.61e-20 | 93.2 |
| MS.gene51240.t1 | AT2G30500 | 27.681 | 401 | 208 | 13 | 115 | 507 | 188 | 514 | 6.61e-20 | 93.2 |
| MS.gene51240.t1 | AT2G30500 | 27.681 | 401 | 208 | 13 | 115 | 507 | 187 | 513 | 1.01e-19 | 92.4 |
| MS.gene51240.t1 | AT3G10880 | 55.435 | 92 | 40 | 1 | 396 | 486 | 188 | 279 | 2.16e-18 | 85.9 |
| MS.gene51240.t1 | AT3G10880 | 55.435 | 92 | 40 | 1 | 396 | 486 | 188 | 279 | 2.16e-18 | 85.9 |
| MS.gene51240.t1 | AT3G10880 | 55.435 | 92 | 40 | 1 | 396 | 486 | 226 | 317 | 3.81e-18 | 85.9 |
| MS.gene51240.t1 | AT3G10880 | 55.435 | 92 | 40 | 1 | 396 | 486 | 226 | 317 | 3.81e-18 | 85.9 |
| MS.gene51240.t1 | AT5G58320 | 24.501 | 351 | 223 | 8 | 8 | 338 | 70 | 398 | 3.15e-11 | 65.9 |
| MS.gene51240.t1 | AT5G58320 | 24.501 | 351 | 223 | 8 | 8 | 338 | 113 | 441 | 9.06e-11 | 64.7 |
| MS.gene51240.t1 | AT5G58320 | 24.501 | 351 | 223 | 8 | 8 | 338 | 113 | 441 | 9.06e-11 | 64.7 |
Find 91 sgRNAs with CRISPR-Local
Find 101 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TCCAATTGTTCCACCAAATT+TGG | 0.117641 | 6.3:+76301785 | None:intergenic |
| TTAACTTGTTTGCTAATAAA+TGG | 0.193584 | 6.3:+76301396 | None:intergenic |
| ATGGAGGGCACTGGTGATTT+TGG | 0.209159 | 6.3:-76302379 | MS.gene51240:CDS |
| GGAGACTCATGTTCCAAATT+TGG | 0.236051 | 6.3:-76301798 | MS.gene51240:CDS |
| TGAAAGCTTAAGTTTCAAAT+TGG | 0.275719 | 6.3:+76301967 | None:intergenic |
| AGGCGAAATCAGTTCTTTAA+AGG | 0.292177 | 6.3:-76301309 | MS.gene51240:CDS |
| GGATGAACTTGAGCATAAAA+AGG | 0.292373 | 6.3:-76301858 | MS.gene51240:CDS |
| GCGATGTGAAGCTGAAAATT+TGG | 0.295896 | 6.3:-76301372 | MS.gene51240:CDS |
| AGACGATCAAATAGAAAATA+TGG | 0.304491 | 6.3:-76301144 | MS.gene51240:CDS |
| TGGTCCTCAACCCGTCAATT+TGG | 0.305441 | 6.3:-76302281 | MS.gene51240:CDS |
| TCCAAATTTGGTGGAACAAT+TGG | 0.319106 | 6.3:-76301786 | MS.gene51240:CDS |
| TTAGAATGGCAGCTTGATTC+AGG | 0.324320 | 6.3:-76301632 | MS.gene51240:CDS |
| GAAAGAATTAGCACTGAATA+AGG | 0.325177 | 6.3:-76301879 | MS.gene51240:CDS |
| TGATTTGGAAGCTGTGAATA+AGG | 0.353331 | 6.3:-76301264 | MS.gene51240:CDS |
| TCATCAGAGAAGTAATGATT+TGG | 0.361754 | 6.3:-76301279 | MS.gene51240:CDS |
| TTCTGGTATGGAGTATAATA+TGG | 0.373850 | 6.3:+76302322 | None:intergenic |
| CACTCTCTTGTGCGCGAAGC+TGG | 0.374202 | 6.3:+76301108 | None:intergenic |
| ACTTCTGCAATCCCTGCTTC+TGG | 0.382177 | 6.3:+76302305 | None:intergenic |
| AAAGACAAAACCAGTTTGTT+GGG | 0.390775 | 6.3:-76302060 | MS.gene51240:CDS |
| ATTTATTAGCAAACAAGTTA+AGG | 0.393675 | 6.3:-76301394 | MS.gene51240:CDS |
| TGCAATCCCTGCTTCTGGTA+TGG | 0.419352 | 6.3:+76302310 | None:intergenic |
| TTTCTCCCTCCCCAACAAAC+TGG | 0.427170 | 6.3:+76302050 | None:intergenic |
| GAAAGACAAAACCAGTTTGT+TGG | 0.429529 | 6.3:-76302061 | MS.gene51240:CDS |
| CAAGTGAGAAACACAGTTGC+CGG | 0.431096 | 6.3:+76300952 | None:intergenic |
| TTCTTTCAGCTTACCTTGAG+TGG | 0.435903 | 6.3:+76302099 | None:intergenic |
| GACCTCAACACTGGAGGAAT+GGG | 0.440516 | 6.3:-76301429 | MS.gene51240:CDS |
| TGCCACGATTGATAAACTTA+AGG | 0.449422 | 6.3:-76301183 | MS.gene51240:CDS |
| AAGTCCAAATTGACGGGTTG+AGG | 0.461874 | 6.3:+76302277 | None:intergenic |
| TTGGACTTCTCTCCTAGCTC+AGG | 0.462175 | 6.3:-76302262 | MS.gene51240:CDS |
| AAGAGAAGAGGGAGGCAATC+CGG | 0.472016 | 6.3:-76300971 | MS.gene51240:CDS |
| AAATTTGGTGGAACAATTGG+AGG | 0.473827 | 6.3:-76301783 | MS.gene51240:CDS |
| TGAAACTAACCTTGAAATTG+AGG | 0.476440 | 6.3:-76301558 | MS.gene51240:CDS |
| GCTGTATGCTACACAACAAA+TGG | 0.476775 | 6.3:-76301339 | MS.gene51240:CDS |
| AAGCAGGGATTGCAGAAGTC+TGG | 0.482361 | 6.3:-76302301 | MS.gene51240:CDS |
| TCTTGACAAAGAAAGTTCAT+TGG | 0.485728 | 6.3:-76302350 | MS.gene51240:CDS |
| TTTCCTGTGCCACTTCAAGC+TGG | 0.485925 | 6.3:+76301666 | None:intergenic |
| AAATTTGCGAGTTGGAAGAC+AGG | 0.492430 | 6.3:-76301601 | MS.gene51240:CDS |
| TAGCTCTGCCGCTTCTCAAA+AGG | 0.495879 | 6.3:-76302233 | MS.gene51240:CDS |
| CTTCATCATTTGATATCTTG+AGG | 0.496814 | 6.3:+76301747 | None:intergenic |
| TGACCTCAACACTGGAGGAA+TGG | 0.500142 | 6.3:-76301430 | MS.gene51240:CDS |
| CAATCTCAGATGGAGGGCAC+TGG | 0.502676 | 6.3:-76302388 | None:intergenic |
| CAGAAAGCAAATTTGCGAGT+TGG | 0.505145 | 6.3:-76301609 | MS.gene51240:CDS |
| GCAAGATTAAGAAAGGAACT+TGG | 0.511927 | 6.3:-76301722 | MS.gene51240:CDS |
| CGCGAAGCTGGATAAGCTCT+CGG | 0.518288 | 6.3:+76301120 | None:intergenic |
| CTCTGCCGCTTCTCAAAAGG+AGG | 0.519691 | 6.3:-76302230 | MS.gene51240:CDS |
| TTATACTCCATACCAGAAGC+AGG | 0.522141 | 6.3:-76302317 | MS.gene51240:CDS |
| CTATAACGGTATGACTCCTA+TGG | 0.524652 | 6.3:+76300883 | None:intergenic |
| GAATATTGTGATCTCCGATC+GGG | 0.528397 | 6.3:-76300997 | MS.gene51240:CDS |
| AGCCTTAAGTTTATCAATCG+TGG | 0.528872 | 6.3:+76301181 | None:intergenic |
| TGAAGTTGCAAGATTAAGAA+AGG | 0.532063 | 6.3:-76301729 | MS.gene51240:CDS |
| CAGCTGAATGTAACTTTAGG+AGG | 0.534506 | 6.3:+76300860 | None:intergenic |
| CAAGATTAAGAAAGGAACTT+GGG | 0.540440 | 6.3:-76301721 | MS.gene51240:CDS |
| AGTTGGAAGACAGGATTACA+TGG | 0.540635 | 6.3:-76301592 | MS.gene51240:CDS |
| AAAATGTGACTAAATTAGAA+TGG | 0.541639 | 6.3:-76301646 | MS.gene51240:CDS |
| CAAAACCAGTTTGTTGGGGA+GGG | 0.542348 | 6.3:-76302055 | MS.gene51240:CDS |
| GGATCAAATGCATGATGTGC+AGG | 0.543842 | 6.3:-76301522 | MS.gene51240:CDS |
| AGGAGAGAAGTCCAAATTGA+CGG | 0.546664 | 6.3:+76302270 | None:intergenic |
| AGACCAGCTTGAAGTGGCAC+AGG | 0.547887 | 6.3:-76301669 | MS.gene51240:CDS |
| CCTCTTCTCTTCATCCCGAT+CGG | 0.548058 | 6.3:+76300983 | None:intergenic |
| AGAGTCAGTTTCAGAATCAG+AGG | 0.550792 | 6.3:+76302180 | None:intergenic |
| GAGCTAACACCACCTGAGCT+AGG | 0.552383 | 6.3:+76302250 | None:intergenic |
| GTAACTTTAGGAGGCTATAA+CGG | 0.556469 | 6.3:+76300869 | None:intergenic |
| AACACAATTGACCTCAACAC+TGG | 0.559176 | 6.3:-76301438 | MS.gene51240:CDS |
| GACTCATGTTCCAAATTTGG+TGG | 0.560411 | 6.3:-76301795 | MS.gene51240:CDS |
| GGAGAGAAGTCCAAATTGAC+GGG | 0.568151 | 6.3:+76302271 | None:intergenic |
| CAGTATTCACCGCCGTGCCA+TGG | 0.572576 | 6.3:+76302134 | None:intergenic |
| GACGATGAAGGAACCACTCA+AGG | 0.572786 | 6.3:-76302112 | MS.gene51240:CDS |
| TTTGGTGGAACAATTGGAGG+TGG | 0.574711 | 6.3:-76301780 | MS.gene51240:CDS |
| CTCCCATTCCTCCAGTGTTG+AGG | 0.578084 | 6.3:+76301427 | None:intergenic |
| ACAAAACCAGTTTGTTGGGG+AGG | 0.578682 | 6.3:-76302056 | MS.gene51240:CDS |
| CGATCGGGATGAAGAGAAGA+GGG | 0.583720 | 6.3:-76300982 | MS.gene51240:CDS |
| GACTTCTCTCCTAGCTCAGG+TGG | 0.584402 | 6.3:-76302259 | MS.gene51240:CDS |
| TCACTTGATCACTACAAGAG+TGG | 0.587379 | 6.3:-76300936 | MS.gene51240:CDS |
| TTTGTCTTTCGGTATCAGCA+AGG | 0.592493 | 6.3:+76302074 | None:intergenic |
| AGAATATTGTGATCTCCGAT+CGG | 0.598633 | 6.3:-76300998 | MS.gene51240:CDS |
| ATTGCAAGACCAGCTTGAAG+TGG | 0.606605 | 6.3:-76301675 | MS.gene51240:CDS |
| AAGACAAAACCAGTTTGTTG+GGG | 0.607800 | 6.3:-76302059 | MS.gene51240:CDS |
| TCCTCACAATCTCAGATGGA+GGG | 0.612527 | 6.3:-76302394 | None:intergenic |
| CCGATCGGGATGAAGAGAAG+AGG | 0.612539 | 6.3:-76300983 | MS.gene51240:CDS |
| GAATCAAAATGAGAAAAGTG+AGG | 0.626506 | 6.3:-76301921 | MS.gene51240:CDS |
| AATCAAAATGAGAAAAGTGA+GGG | 0.627000 | 6.3:-76301920 | MS.gene51240:CDS |
| GTCAGTTTCAGAATCAGAGG+AGG | 0.631262 | 6.3:+76302183 | None:intergenic |
| TGTAATTCATTACCATGGCA+CGG | 0.633012 | 6.3:-76302146 | MS.gene51240:CDS |
| ACACAACAAATGGTTATGCA+AGG | 0.640355 | 6.3:-76301329 | MS.gene51240:CDS |
| GCGGTGAATACTGACGATGA+AGG | 0.643067 | 6.3:-76302124 | MS.gene51240:CDS |
| TATACTCCATACCAGAAGCA+GGG | 0.646612 | 6.3:-76302316 | MS.gene51240:CDS |
| ACAATTGACCTCAACACTGG+AGG | 0.650713 | 6.3:-76301435 | MS.gene51240:CDS |
| AAGTCTGTAATTCATTACCA+TGG | 0.664113 | 6.3:-76302151 | MS.gene51240:CDS |
| TCGGGATGAAGAGAAGAGGG+AGG | 0.677476 | 6.3:-76300979 | MS.gene51240:CDS |
| TCTGCCGCTTCTCAAAAGGA+GGG | 0.690022 | 6.3:-76302229 | MS.gene51240:CDS |
| AATTCATTACCATGGCACGG+CGG | 0.798519 | 6.3:-76302143 | MS.gene51240:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AAAATGTGACTAAATTAGAA+TGG | - | chr6.3:76301608-76301627 | MS.gene51240:CDS | 20.0% |
| !! | ATTTATTAGCAAACAAGTTA+AGG | - | chr6.3:76301860-76301879 | MS.gene51240:CDS | 20.0% |
| !!! | TTAACTTGTTTGCTAATAAA+TGG | + | chr6.3:76301861-76301880 | None:intergenic | 20.0% |
| ! | AATCAAAATGAGAAAAGTGA+GGG | - | chr6.3:76301334-76301353 | MS.gene51240:CDS | 25.0% |
| ! | AGACGATCAAATAGAAAATA+TGG | - | chr6.3:76302110-76302129 | MS.gene51240:CDS | 25.0% |
| ! | GAAGAAGTTCATTAAAAATG+AGG | - | chr6.3:76301249-76301268 | MS.gene51240:CDS | 25.0% |
| ! | TGAAAGCTTAAGTTTCAAAT+TGG | + | chr6.3:76301290-76301309 | None:intergenic | 25.0% |
| AAGTCTGTAATTCATTACCA+TGG | - | chr6.3:76301103-76301122 | MS.gene51240:CDS | 30.0% | |
| AGTAGAAGAACTTGAAAAAG+AGG | - | chr6.3:76302224-76302243 | MS.gene51240:CDS | 30.0% | |
| CAAGATTAAGAAAGGAACTT+GGG | - | chr6.3:76301533-76301552 | MS.gene51240:CDS | 30.0% | |
| CTTCATCATTTGATATCTTG+AGG | + | chr6.3:76301510-76301529 | None:intergenic | 30.0% | |
| GAATCAAAATGAGAAAAGTG+AGG | - | chr6.3:76301333-76301352 | MS.gene51240:CDS | 30.0% | |
| GATGAACTTGAGCATAAAAA+GGG | - | chr6.3:76301397-76301416 | MS.gene51240:CDS | 30.0% | |
| TCATCAGAGAAGTAATGATT+TGG | - | chr6.3:76301975-76301994 | MS.gene51240:CDS | 30.0% | |
| TCTTGACAAAGAAAGTTCAT+TGG | - | chr6.3:76300904-76300923 | MS.gene51240:CDS | 30.0% | |
| TGAAACTAACCTTGAAATTG+AGG | - | chr6.3:76301696-76301715 | MS.gene51240:CDS | 30.0% | |
| TGAAGTTGCAAGATTAAGAA+AGG | - | chr6.3:76301525-76301544 | MS.gene51240:CDS | 30.0% | |
| TTCTGGTATGGAGTATAATA+TGG | + | chr6.3:76300935-76300954 | None:intergenic | 30.0% | |
| ! | AAAGACAAAACCAGTTTGTT+GGG | - | chr6.3:76301194-76301213 | MS.gene51240:CDS | 30.0% |
| ! | GAAAGAATTAGCACTGAATA+AGG | - | chr6.3:76301375-76301394 | MS.gene51240:CDS | 30.0% |
| ! | GAAATTCTTCAAGCTTTTAC+TGG | - | chr6.3:76302348-76302367 | MS.gene51240:CDS | 30.0% |
| !!! | AACAAACTGGTTTTGTCTTT+CGG | + | chr6.3:76301194-76301213 | None:intergenic | 30.0% |
| AATTGAGGTGCAAAAACTGA+AGG | - | chr6.3:76301711-76301730 | MS.gene51240:CDS | 35.0% | |
| ACACAACAAATGGTTATGCA+AGG | - | chr6.3:76301925-76301944 | MS.gene51240:CDS | 35.0% | |
| AGAATATTGTGATCTCCGAT+CGG | - | chr6.3:76302256-76302275 | MS.gene51240:CDS | 35.0% | |
| AGCCTTAAGTTTATCAATCG+TGG | + | chr6.3:76302076-76302095 | None:intergenic | 35.0% | |
| AGGCGAAATCAGTTCTTTAA+AGG | - | chr6.3:76301945-76301964 | MS.gene51240:CDS | 35.0% | |
| GAAAGACAAAACCAGTTTGT+TGG | - | chr6.3:76301193-76301212 | MS.gene51240:CDS | 35.0% | |
| GCAAGATTAAGAAAGGAACT+TGG | - | chr6.3:76301532-76301551 | MS.gene51240:CDS | 35.0% | |
| GGATGAACTTGAGCATAAAA+AGG | - | chr6.3:76301396-76301415 | MS.gene51240:CDS | 35.0% | |
| TCCAATTGTTCCACCAAATT+TGG | + | chr6.3:76301472-76301491 | None:intergenic | 35.0% | |
| TGCCACGATTGATAAACTTA+AGG | - | chr6.3:76302071-76302090 | MS.gene51240:CDS | 35.0% | |
| TGTAATTCATTACCATGGCA+CGG | - | chr6.3:76301108-76301127 | MS.gene51240:CDS | 35.0% | |
| ! | AAATTTGGTGGAACAATTGG+AGG | - | chr6.3:76301471-76301490 | MS.gene51240:CDS | 35.0% |
| ! | GTTTTTGCACCTCAATTTCA+AGG | + | chr6.3:76301708-76301727 | None:intergenic | 35.0% |
| ! | TCCAAATTTGGTGGAACAAT+TGG | - | chr6.3:76301468-76301487 | MS.gene51240:CDS | 35.0% |
| ! | TGATTTGGAAGCTGTGAATA+AGG | - | chr6.3:76301990-76302009 | MS.gene51240:CDS | 35.0% |
| !! | AAGACAAAACCAGTTTGTTG+GGG | - | chr6.3:76301195-76301214 | MS.gene51240:CDS | 35.0% |
| !! | CTGTGAATAAGGATTTTGAC+AGG | - | chr6.3:76302001-76302020 | MS.gene51240:CDS | 35.0% |
| AACACAATTGACCTCAACAC+TGG | - | chr6.3:76301816-76301835 | MS.gene51240:CDS | 40.0% | |
| ACTTGAGCATAAAAAGGGAC+AGG | - | chr6.3:76301402-76301421 | MS.gene51240:CDS | 40.0% | |
| AGAGTCAGTTTCAGAATCAG+AGG | + | chr6.3:76301077-76301096 | None:intergenic | 40.0% | |
| AGGAGAGAAGTCCAAATTGA+CGG | + | chr6.3:76300987-76301006 | None:intergenic | 40.0% | |
| AGTTGGAAGACAGGATTACA+TGG | - | chr6.3:76301662-76301681 | MS.gene51240:CDS | 40.0% | |
| CAGAAAGCAAATTTGCGAGT+TGG | - | chr6.3:76301645-76301664 | MS.gene51240:CDS | 40.0% | |
| CTATAACGGTATGACTCCTA+TGG | + | chr6.3:76302374-76302393 | None:intergenic | 40.0% | |
| GAATATTGTGATCTCCGATC+GGG | - | chr6.3:76302257-76302276 | MS.gene51240:CDS | 40.0% | |
| GACTCATGTTCCAAATTTGG+TGG | - | chr6.3:76301459-76301478 | MS.gene51240:CDS | 40.0% | |
| GCAAAAGCAAAAAGCTGAGT+TGG | - | chr6.3:76301435-76301454 | MS.gene51240:CDS | 40.0% | |
| GCGATGTGAAGCTGAAAATT+TGG | - | chr6.3:76301882-76301901 | MS.gene51240:CDS | 40.0% | |
| GCTGTATGCTACACAACAAA+TGG | - | chr6.3:76301915-76301934 | MS.gene51240:CDS | 40.0% | |
| GGAGACTCATGTTCCAAATT+TGG | - | chr6.3:76301456-76301475 | MS.gene51240:CDS | 40.0% | |
| TATACTCCATACCAGAAGCA+GGG | - | chr6.3:76300938-76300957 | MS.gene51240:CDS | 40.0% | |
| TCACTTGATCACTACAAGAG+TGG | - | chr6.3:76302318-76302337 | MS.gene51240:CDS | 40.0% | |
| TTAGAATGGCAGCTTGATTC+AGG | - | chr6.3:76301622-76301641 | MS.gene51240:CDS | 40.0% | |
| TTATACTCCATACCAGAAGC+AGG | - | chr6.3:76300937-76300956 | MS.gene51240:CDS | 40.0% | |
| TTCTTTCAGCTTACCTTGAG+TGG | + | chr6.3:76301158-76301177 | None:intergenic | 40.0% | |
| TTTGTCTTTCGGTATCAGCA+AGG | + | chr6.3:76301183-76301202 | None:intergenic | 40.0% | |
| ! | AAATTTGCGAGTTGGAAGAC+AGG | - | chr6.3:76301653-76301672 | MS.gene51240:CDS | 40.0% |
| ! | TTCAAGCTTTTACTGGCCAT+AGG | - | chr6.3:76302355-76302374 | MS.gene51240:CDS | 40.0% |
| AATTCATTACCATGGCACGG+CGG | - | chr6.3:76301111-76301130 | MS.gene51240:CDS | 45.0% | |
| ACAATTGACCTCAACACTGG+AGG | - | chr6.3:76301819-76301838 | MS.gene51240:CDS | 45.0% | |
| ATTGCAAGACCAGCTTGAAG+TGG | - | chr6.3:76301579-76301598 | MS.gene51240:CDS | 45.0% | |
| CAAGTGAGAAACACAGTTGC+CGG | + | chr6.3:76302305-76302324 | None:intergenic | 45.0% | |
| GGAGAGAAGTCCAAATTGAC+GGG | + | chr6.3:76300986-76301005 | None:intergenic | 45.0% | |
| GGATCAAATGCATGATGTGC+AGG | - | chr6.3:76301732-76301751 | MS.gene51240:CDS | 45.0% | |
| GTCAGTTTCAGAATCAGAGG+AGG | + | chr6.3:76301074-76301093 | None:intergenic | 45.0% | |
| TTTGGTGGAACAATTGGAGG+TGG | - | chr6.3:76301474-76301493 | MS.gene51240:CDS | 45.0% | |
| !! | AAGTCCAAATTGACGGGTTG+AGG | + | chr6.3:76300980-76300999 | None:intergenic | 45.0% |
| !! | ACAAAACCAGTTTGTTGGGG+AGG | - | chr6.3:76301198-76301217 | MS.gene51240:CDS | 45.0% |
| !! | AGAACCCTCCTTTTGAGAAG+CGG | + | chr6.3:76301032-76301051 | None:intergenic | 45.0% |
| !! | CAAAACCAGTTTGTTGGGGA+GGG | - | chr6.3:76301199-76301218 | MS.gene51240:CDS | 45.0% |
| AAGAGAAGAGGGAGGCAATC+CGG | - | chr6.3:76302283-76302302 | MS.gene51240:CDS | 50.0% | |
| AAGCAGGGATTGCAGAAGTC+TGG | - | chr6.3:76300953-76300972 | MS.gene51240:CDS | 50.0% | |
| ACTTCTGCAATCCCTGCTTC+TGG | + | chr6.3:76300952-76300971 | None:intergenic | 50.0% | |
| CCTCTTCTCTTCATCCCGAT+CGG | + | chr6.3:76302274-76302293 | None:intergenic | 50.0% | |
| CGATCGGGATGAAGAGAAGA+GGG | - | chr6.3:76302272-76302291 | MS.gene51240:CDS | 50.0% | |
| GACCTCAACACTGGAGGAAT+GGG | - | chr6.3:76301825-76301844 | MS.gene51240:CDS | 50.0% | |
| GACGATGAAGGAACCACTCA+AGG | - | chr6.3:76301142-76301161 | MS.gene51240:CDS | 50.0% | |
| GCATAAAAAGGGACAGGTGC+TGG | - | chr6.3:76301408-76301427 | MS.gene51240:CDS | 50.0% | |
| GCGGTGAATACTGACGATGA+AGG | - | chr6.3:76301130-76301149 | MS.gene51240:CDS | 50.0% | |
| TGACCTCAACACTGGAGGAA+TGG | - | chr6.3:76301824-76301843 | MS.gene51240:CDS | 50.0% | |
| TGCAATCCCTGCTTCTGGTA+TGG | + | chr6.3:76300947-76300966 | None:intergenic | 50.0% | |
| TGGTCCTCAACCCGTCAATT+TGG | - | chr6.3:76300973-76300992 | MS.gene51240:CDS | 50.0% | |
| TTGGACTTCTCTCCTAGCTC+AGG | - | chr6.3:76300992-76301011 | MS.gene51240:CDS | 50.0% | |
| TTTCTCCCTCCCCAACAAAC+TGG | + | chr6.3:76301207-76301226 | None:intergenic | 50.0% | |
| ! | TAGCTCTGCCGCTTCTCAAA+AGG | - | chr6.3:76301021-76301040 | MS.gene51240:CDS | 50.0% |
| ! | TCTGCCGCTTCTCAAAAGGA+GGG | - | chr6.3:76301025-76301044 | MS.gene51240:CDS | 50.0% |
| !! | TTTCCTGTGCCACTTCAAGC+TGG | + | chr6.3:76301591-76301610 | None:intergenic | 50.0% |
| !!! | ATGGAGGGCACTGGTGATTT+TGG | - | chr6.3:76300875-76300894 | MS.gene51240:CDS | 50.0% |
| AGACCAGCTTGAAGTGGCAC+AGG | - | chr6.3:76301585-76301604 | MS.gene51240:CDS | 55.0% | |
| CCGATCGGGATGAAGAGAAG+AGG | - | chr6.3:76302271-76302290 | MS.gene51240:CDS | 55.0% | |
| CGCGAAGCTGGATAAGCTCT+CGG | + | chr6.3:76302137-76302156 | None:intergenic | 55.0% | |
| CTCCCATTCCTCCAGTGTTG+AGG | + | chr6.3:76301830-76301849 | None:intergenic | 55.0% | |
| GACTTCTCTCCTAGCTCAGG+TGG | - | chr6.3:76300995-76301014 | MS.gene51240:CDS | 55.0% | |
| GAGCTAACACCACCTGAGCT+AGG | + | chr6.3:76301007-76301026 | None:intergenic | 55.0% | |
| TCGGGATGAAGAGAAGAGGG+AGG | - | chr6.3:76302275-76302294 | MS.gene51240:CDS | 55.0% | |
| ! | CTCTGCCGCTTCTCAAAAGG+AGG | - | chr6.3:76301024-76301043 | MS.gene51240:CDS | 55.0% |
| !!! | GGCACTGGTGATTTTGGCGA+AGG | - | chr6.3:76300881-76300900 | MS.gene51240:CDS | 55.0% |
| CACTCTCTTGTGCGCGAAGC+TGG | + | chr6.3:76302149-76302168 | None:intergenic | 60.0% | |
| CAGTATTCACCGCCGTGCCA+TGG | + | chr6.3:76301123-76301142 | None:intergenic | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr6.3 | gene | 76300875 | 76302401 | 76300875 | ID=MS.gene51240 |
| chr6.3 | mRNA | 76300875 | 76302401 | 76300875 | ID=MS.gene51240.t1;Parent=MS.gene51240 |
| chr6.3 | exon | 76300875 | 76302401 | 76300875 | ID=MS.gene51240.t1.exon1;Parent=MS.gene51240.t1 |
| chr6.3 | CDS | 76300875 | 76302401 | 76300875 | ID=cds.MS.gene51240.t1;Parent=MS.gene51240.t1 |
| Gene Sequence |
| Protein sequence |
