AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene23437


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene23437.t1 MTR_7g098290 98.571 140 2 0 1 140 1 140 3.93e-98 278
MS.gene23437.t1 MTR_1g069585 98.571 140 2 0 1 140 1 140 3.93e-98 278
MS.gene23437.t1 MTR_7g098290 98.400 125 2 0 16 140 1 125 8.58e-87 249
MS.gene23437.t1 MTR_1g026090 81.481 135 10 1 2 136 42 161 3.07e-71 212
MS.gene23437.t1 MTR_5g054560 68.800 125 15 1 16 140 1 101 1.78e-53 164
MS.gene23437.t1 MTR_5g464620 89.796 49 5 0 14 62 16 64 3.73e-25 91.7
MS.gene23437.t1 MTR_2g070580 92.683 41 3 0 100 140 87 127 2.62e-21 83.2
MS.gene23437.t1 MTR_1779s0020 36.522 115 65 3 26 136 8 118 3.65e-16 70.1
MS.gene23437.t1 MTR_4g051390 36.522 115 65 3 26 136 8 118 4.98e-16 69.7
MS.gene23437.t1 MTR_2g087550 36.792 106 60 2 26 128 8 109 1.55e-14 67.8
MS.gene23437.t1 MTR_0002s0780 34.426 122 73 3 23 140 132 250 1.92e-14 68.2
MS.gene23437.t1 MTR_4g017930 80.556 36 5 1 104 139 13 46 1.09e-11 58.5
MS.gene23437.t1 MTR_4g066540 72.500 40 7 1 100 139 54 89 7.28e-11 55.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene23437.t1 AT3G04400 98.571 140 2 0 1 140 1 140 1.65e-98 279
MS.gene23437.t1 AT2G33370 98.571 140 2 0 1 140 1 140 1.65e-98 279
MS.gene23437.t1 AT1G04480 98.571 140 2 0 1 140 1 140 1.65e-98 279
MS.gene23437.t1 AT3G04400 100.000 125 0 0 16 140 1 125 4.64e-88 252
MS.gene23437.t1 AT2G33370 100.000 125 0 0 16 140 1 125 4.64e-88 252
MS.gene23437.t1 ATCG00780 34.783 115 67 3 26 136 8 118 4.97e-15 67.0

Find 51 sgRNAs with CRISPR-Local

Find 75 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTTACCTTTCATTTCTCCTT+TGG 0.232028 7.3:-13836665 None:intergenic
TTACCTTTCATTTCTCCTTT+GGG 0.290146 7.3:-13836664 None:intergenic
ATTCTTAGCTCCGGTGTTAT+CGG 0.357276 7.3:-13836315 None:intergenic
ACATGAAAACACCGTCCTTT+CGG 0.371684 7.3:-13836496 None:intergenic
GATCCGCGGGTAACAAGTTC+AGG 0.386005 7.3:+13836252 MS.gene23437:CDS
GGAAGGAGTGTGCTGATCTA+TGG 0.394494 7.3:+13837091 MS.gene23437:CDS
CCTTCCTTTGATACCTTTGA+CGG 0.396996 7.3:-13836351 None:intergenic
AGCACCTTCTTACGGAGATC+AGG 0.422915 7.3:-13836437 None:intergenic
TTCTGCTTGCGTTGGTGACA+TGG 0.443666 7.3:+13836388 MS.gene23437:CDS
CAATTCACCGTCGCCGCCAC+TGG 0.459295 7.3:-13836290 None:intergenic
TTAGCAGCACTTGCAATCCT+AGG 0.471348 7.3:-13837114 None:intergenic
CTCTACATCATTTCCGTCAA+AGG 0.478393 7.3:+13836338 MS.gene23437:CDS
GGTGATGGCCACCGTGAAGA+AGG 0.480460 7.3:+13836409 MS.gene23437:CDS
CGTTTGCCTTCTGCTTGCGT+TGG 0.482632 7.3:+13836380 MS.gene23437:CDS
TCTGCTATCACTGGTCCAAT+CGG 0.485775 7.3:+13837069 MS.gene23437:CDS
TAACAAGTTCAGGATGTCAC+TGG 0.500497 7.3:+13836262 MS.gene23437:CDS
CGGCAGGAAGCACCTTCTTA+CGG 0.502055 7.3:-13836445 None:intergenic
ATCAGGCTTACCCTTCTTCA+CGG 0.504036 7.3:-13836420 None:intergenic
CTCAGGTCGCGGAGGATCCG+CGG 0.504873 7.3:+13836238 MS.gene23437:intron
CTGCTATCACTGGTCCAATC+GGG 0.511052 7.3:+13837070 MS.gene23437:CDS
TTGTATTGTGCAGACAATGC+TGG 0.531818 7.3:+13836628 MS.gene23437:intron
ATTGTAGGGTCTGCTATCAC+TGG 0.534637 7.3:+13837060 MS.gene23437:intron
CAACGCAAGCAGAAGGCAAA+CGG 0.538758 7.3:-13836379 None:intergenic
TCAGCACACTCCTTCCCGAT+TGG 0.539409 7.3:-13837084 None:intergenic
CGCTGGCGAACGATGACGGC+AGG 0.540043 7.3:-13836461 None:intergenic
ACACCGTCCTTTCGGCGCCA+TGG 0.550488 7.3:-13836488 None:intergenic
CCGTCAAAGGTATCAAAGGA+AGG 0.551618 7.3:+13836351 MS.gene23437:CDS
TCAGGTCGCGGAGGATCCGC+GGG 0.560803 7.3:+13836239 MS.gene23437:intron
AGTGTGCTGATCTATGGCCT+AGG 0.561029 7.3:+13837097 MS.gene23437:CDS
TTCGGCGCCATGGTTTACGC+TGG 0.563205 7.3:-13836478 None:intergenic
GGTGTTATTGTTAATCCCAA+AGG 0.565013 7.3:+13836649 MS.gene23437:CDS
TAAGCCTGATCTCCGTAAGA+AGG 0.569085 7.3:+13836433 MS.gene23437:CDS
ATTTCCGTCAAAGGTATCAA+AGG 0.575410 7.3:+13836347 MS.gene23437:CDS
GGGTCTACCAGTGGCGGCGA+CGG 0.576990 7.3:+13836283 MS.gene23437:CDS
GTGATGGCCACCGTGAAGAA+GGG 0.579374 7.3:+13836410 MS.gene23437:CDS
TTGCGTTGGTGACATGGTGA+TGG 0.588839 7.3:+13836394 MS.gene23437:CDS
GATGTAGAGATTCTTAGCTC+CGG 0.596136 7.3:-13836324 None:intergenic
TTTACGCTGGCGAACGATGA+CGG 0.598744 7.3:-13836465 None:intergenic
AAACCATGGCGCCGAAAGGA+CGG 0.604162 7.3:+13836485 MS.gene23437:CDS
GCGTAAACCATGGCGCCGAA+AGG 0.604166 7.3:+13836481 MS.gene23437:CDS
AACAAGTTCAGGATGTCACT+GGG 0.607031 7.3:+13836263 MS.gene23437:CDS
ATGTCACCAACGCAAGCAGA+AGG 0.609870 7.3:-13836386 None:intergenic
GTCACTGGGTCTACCAGTGG+CGG 0.621290 7.3:+13836277 MS.gene23437:CDS
GTGAATTGCGCCGATAACAC+CGG 0.624326 7.3:+13836305 MS.gene23437:CDS
AATCCCAAAGGAGAAATGAA+AGG 0.624552 7.3:+13836661 MS.gene23437:CDS
TCGTTCGCCAGCGTAAACCA+TGG 0.647146 7.3:+13836471 MS.gene23437:CDS
GCAGCAGCAATGTCGAAGAG+AGG 0.651240 7.3:+13836125 None:intergenic
GATGTCACTGGGTCTACCAG+TGG 0.653286 7.3:+13836274 MS.gene23437:CDS
TATCACTGGTCCAATCGGGA+AGG 0.675150 7.3:+13837074 MS.gene23437:CDS
CATCCTGAACTTGTTACCCG+CGG 0.683379 7.3:-13836255 None:intergenic
AGGCTTACCCTTCTTCACGG+TGG 0.718796 7.3:-13836417 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TTAATAATCAGTATTAAAAT+TGG + chr7.3:13836938-13836957 MS.gene23437:intron 10.0%
!! TAATCAGTATTAAAATTGGT+TGG + chr7.3:13836942-13836961 MS.gene23437:intron 20.0%
!! TGATTCTTACTTAATATTTC+TGG + chr7.3:13836604-13836623 MS.gene23437:intron 20.0%
! TGGGTAATATATGTAATGAT+TGG + chr7.3:13836756-13836775 MS.gene23437:intron 25.0%
!! AATGATTGGTGTTTAATAAG+TGG + chr7.3:13836770-13836789 MS.gene23437:intron 25.0%
!! ATTTTCATCTTCAGATTCTT+AGG + chr7.3:13836157-13836176 MS.gene23437:intron 25.0%
ATGATAATATCGATGTAGGT+GGG + chr7.3:13836737-13836756 MS.gene23437:intron 30.0%
ATTAAAATTGGTTGGAGGAT+CGG + chr7.3:13836950-13836969 MS.gene23437:intron 30.0%
CACAACATCAAAACCAAAAA+CGG - chr7.3:13837002-13837021 None:intergenic 30.0%
GGAATTAGAGTTTAGTAACT+TGG + chr7.3:13836873-13836892 MS.gene23437:intron 30.0%
TCAGTATTAAAATTGGTTGG+AGG + chr7.3:13836945-13836964 MS.gene23437:intron 30.0%
TTACCTTTCATTTCTCCTTT+GGG - chr7.3:13836667-13836686 None:intergenic 30.0%
TTTACCTTTCATTTCTCCTT+TGG - chr7.3:13836668-13836687 None:intergenic 30.0%
! TTATTAAAACGCGCTTTTGT+AGG - chr7.3:13836200-13836219 None:intergenic 30.0%
!! CGATTTTCATTTTGTTTCTC+AGG + chr7.3:13836221-13836240 MS.gene23437:intron 30.0%
!! TATGTTTTTATCGCCGTTTT+TGG + chr7.3:13836986-13837005 MS.gene23437:intron 30.0%
!! TCGTGTTGATTGTAATTGTA+GGG + chr7.3:13837046-13837065 MS.gene23437:intron 30.0%
!! TTCGTGTTGATTGTAATTGT+AGG + chr7.3:13837045-13837064 MS.gene23437:intron 30.0%
AATCCCAAAGGAGAAATGAA+AGG + chr7.3:13836661-13836680 MS.gene23437:CDS 35.0%
ACTGCTGTGTCTTCAATAAT+TGG + chr7.3:13836852-13836871 MS.gene23437:intron 35.0%
CACGATGATAATATCGATGT+AGG + chr7.3:13836733-13836752 MS.gene23437:intron 35.0%
CTACATCGATATTATCATCG+TGG - chr7.3:13836735-13836754 None:intergenic 35.0%
GATGATAATATCGATGTAGG+TGG + chr7.3:13836736-13836755 MS.gene23437:intron 35.0%
TTAAAATTGGTTGGAGGATC+GGG + chr7.3:13836951-13836970 MS.gene23437:intron 35.0%
!! ATTTCCGTCAAAGGTATCAA+AGG + chr7.3:13836347-13836366 MS.gene23437:CDS 35.0%
!! GGTGTTATTGTTAATCCCAA+AGG + chr7.3:13836649-13836668 MS.gene23437:CDS 35.0%
!! GGTTTTGATGTTGTGCTTTT+TGG + chr7.3:13837007-13837026 MS.gene23437:intron 35.0%
AACAAGTTCAGGATGTCACT+GGG + chr7.3:13836263-13836282 MS.gene23437:CDS 40.0%
ACATGAAAACACCGTCCTTT+CGG - chr7.3:13836499-13836518 None:intergenic 40.0%
CCTTCCTTTGATACCTTTGA+CGG - chr7.3:13836354-13836373 None:intergenic 40.0%
CTCTACATCATTTCCGTCAA+AGG + chr7.3:13836338-13836357 MS.gene23437:CDS 40.0%
GATGTAGAGATTCTTAGCTC+CGG - chr7.3:13836327-13836346 None:intergenic 40.0%
TAACAAGTTCAGGATGTCAC+TGG + chr7.3:13836262-13836281 MS.gene23437:CDS 40.0%
TTGTATTGTGCAGACAATGC+TGG + chr7.3:13836628-13836647 MS.gene23437:intron 40.0%
! GGTGTTTTCATGTACTTCGA+AGG + chr7.3:13836506-13836525 MS.gene23437:CDS 40.0%
!! ATTCTTAGCTCCGGTGTTAT+CGG - chr7.3:13836318-13836337 None:intergenic 40.0%
!! TCATTTTGTTTCTCAGGTCG+CGG + chr7.3:13836227-13836246 MS.gene23437:intron 40.0%
ATCAGGCTTACCCTTCTTCA+CGG - chr7.3:13836423-13836442 None:intergenic 45.0%
ATTGTAGGGTCTGCTATCAC+TGG + chr7.3:13837060-13837079 MS.gene23437:intron 45.0%
TAAGCCTGATCTCCGTAAGA+AGG + chr7.3:13836433-13836452 MS.gene23437:CDS 45.0%
TCTGCTATCACTGGTCCAAT+CGG + chr7.3:13837069-13837088 MS.gene23437:CDS 45.0%
TTAGCAGCACTTGCAATCCT+AGG - chr7.3:13837117-13837136 None:intergenic 45.0%
!! CCGTCAAAGGTATCAAAGGA+AGG + chr7.3:13836351-13836370 MS.gene23437:CDS 45.0%
AGCACCTTCTTACGGAGATC+AGG - chr7.3:13836440-13836459 None:intergenic 50.0%
ATGTCACCAACGCAAGCAGA+AGG - chr7.3:13836389-13836408 None:intergenic 50.0%
CAACGCAAGCAGAAGGCAAA+CGG - chr7.3:13836382-13836401 None:intergenic 50.0%
CTGCTATCACTGGTCCAATC+GGG + chr7.3:13837070-13837089 MS.gene23437:CDS 50.0%
TATCACTGGTCCAATCGGGA+AGG + chr7.3:13837074-13837093 MS.gene23437:CDS 50.0%
TTTACGCTGGCGAACGATGA+CGG - chr7.3:13836468-13836487 None:intergenic 50.0%
! AGTGTGCTGATCTATGGCCT+AGG + chr7.3:13837097-13837116 MS.gene23437:CDS 50.0%
! GGAAGGAGTGTGCTGATCTA+TGG + chr7.3:13837091-13837110 MS.gene23437:CDS 50.0%
! GTGAATTGCGCCGATAACAC+CGG + chr7.3:13836305-13836324 MS.gene23437:CDS 50.0%
!! CATCCTGAACTTGTTACCCG+CGG - chr7.3:13836258-13836277 None:intergenic 50.0%
!! TTCTGCTTGCGTTGGTGACA+TGG + chr7.3:13836388-13836407 MS.gene23437:CDS 50.0%
!! TTGCGTTGGTGACATGGTGA+TGG + chr7.3:13836394-13836413 MS.gene23437:CDS 50.0%
!! TTTTGTTTCTCAGGTCGCGG+AGG + chr7.3:13836230-13836249 MS.gene23437:intron 50.0%
AGGCTTACCCTTCTTCACGG+TGG - chr7.3:13836420-13836439 None:intergenic 55.0%
CGTTTGCCTTCTGCTTGCGT+TGG + chr7.3:13836380-13836399 MS.gene23437:CDS 55.0%
GATCCGCGGGTAACAAGTTC+AGG + chr7.3:13836252-13836271 MS.gene23437:CDS 55.0%
GATGTCACTGGGTCTACCAG+TGG + chr7.3:13836274-13836293 MS.gene23437:CDS 55.0%
GTGATGGCCACCGTGAAGAA+GGG + chr7.3:13836410-13836429 MS.gene23437:CDS 55.0%
TCAGCACACTCCTTCCCGAT+TGG - chr7.3:13837087-13837106 None:intergenic 55.0%
TCGTTCGCCAGCGTAAACCA+TGG + chr7.3:13836471-13836490 MS.gene23437:CDS 55.0%
! AAACCATGGCGCCGAAAGGA+CGG + chr7.3:13836485-13836504 MS.gene23437:CDS 55.0%
!! CGGCAGGAAGCACCTTCTTA+CGG - chr7.3:13836448-13836467 None:intergenic 55.0%
GTCACTGGGTCTACCAGTGG+CGG + chr7.3:13836277-13836296 MS.gene23437:CDS 60.0%
TTCGGCGCCATGGTTTACGC+TGG - chr7.3:13836481-13836500 None:intergenic 60.0%
! GCGTAAACCATGGCGCCGAA+AGG + chr7.3:13836481-13836500 MS.gene23437:CDS 60.0%
! GGTGATGGCCACCGTGAAGA+AGG + chr7.3:13836409-13836428 MS.gene23437:CDS 60.0%
ACACCGTCCTTTCGGCGCCA+TGG - chr7.3:13836491-13836510 None:intergenic 65.0%
CAATTCACCGTCGCCGCCAC+TGG - chr7.3:13836293-13836312 None:intergenic 65.0%
CTCAGGTCGCGGAGGATCCG+CGG + chr7.3:13836238-13836257 MS.gene23437:intron 70.0%
GGGTCTACCAGTGGCGGCGA+CGG + chr7.3:13836283-13836302 MS.gene23437:CDS 70.0%
TCAGGTCGCGGAGGATCCGC+GGG + chr7.3:13836239-13836258 MS.gene23437:intron 70.0%
! CGCTGGCGAACGATGACGGC+AGG - chr7.3:13836464-13836483 None:intergenic 70.0%


Chromosome Type Strat End Strand Name
chr7.3 gene 13836134 13837149 13836134 ID=MS.gene23437
chr7.3 mRNA 13836134 13837149 13836134 ID=MS.gene23437.t1;Parent=MS.gene23437
chr7.3 exon 13836134 13836146 13836134 ID=MS.gene23437.t1.exon1;Parent=MS.gene23437.t1
chr7.3 CDS 13836134 13836146 13836134 ID=cds.MS.gene23437.t1;Parent=MS.gene23437.t1
chr7.3 exon 13836243 13836527 13836243 ID=MS.gene23437.t1.exon2;Parent=MS.gene23437.t1
chr7.3 CDS 13836243 13836527 13836243 ID=cds.MS.gene23437.t1;Parent=MS.gene23437.t1
chr7.3 exon 13836641 13836682 13836641 ID=MS.gene23437.t1.exon3;Parent=MS.gene23437.t1
chr7.3 CDS 13836641 13836682 13836641 ID=cds.MS.gene23437.t1;Parent=MS.gene23437.t1
chr7.3 exon 13837067 13837149 13837067 ID=MS.gene23437.t1.exon4;Parent=MS.gene23437.t1
chr7.3 CDS 13837067 13837149 13837067 ID=cds.MS.gene23437.t1;Parent=MS.gene23437.t1
Gene Sequence

>MS.gene23437

ATGTCGAAGAGAGGTTCGTTCTCATTTTCATCTTCAGATTCTTAGGTCAATTAATCACTTTTACCTACAAAAGCGCGTTTTAATAATCGATTTTCATTTTGTTTCTCAGGTCGCGGAGGATCCGCGGGTAACAAGTTCAGGATGTCACTGGGTCTACCAGTGGCGGCGACGGTGAATTGCGCCGATAACACCGGAGCTAAGAATCTCTACATCATTTCCGTCAAAGGTATCAAAGGAAGGTTGAACCGTTTGCCTTCTGCTTGCGTTGGTGACATGGTGATGGCCACCGTGAAGAAGGGTAAGCCTGATCTCCGTAAGAAGGTGCTTCCTGCCGTCATCGTTCGCCAGCGTAAACCATGGCGCCGAAAGGACGGTGTTTTCATGTACTTCGAAGGTATATTCACATTCTTCATTTTTTTTTTGTTTCAAAATTGAGATTAATCTTAATTTGATGTATATGTTAGTGTGAATGATTCTTACTTAATATTTCTGGTTTGTATTGTGCAGACAATGCTGGTGTTATTGTTAATCCCAAAGGAGAAATGAAAGGTAAAAATTCTATATATCTCTTATTAGTTTTGATTCTATATTATTAAATCCACGATGATAATATCGATGTAGGTGGGTAATATATGTAATGATTGGTGTTTAATAAGTGGAATTAGAGCTTAGTAGCTTAGTATATTGAAATTAGAGTTAATAGTTTAGAATTAATAACACTGCTGTGTCTTCAATAATTGGAATTAGAGTTTAGTAACTTGGTGTTAAAATTGAAATTAGAGTTTAATACTTAATAGTTTAGTATTAATAATCAGTATTAAAATTGGTTGGAGGATCGGGTGAGTGTGTGACTATGTTTTTATCGCCGTTTTTGGTTTTGATGTTGTGCTTTTTGGTTCTTCTCTTGATATTTCGTGTTGATTGTAATTGTAGGGTCTGCTATCACTGGTCCAATCGGGAAGGAGTGTGCTGATCTATGGCCTAGGATTGCAAGTGCTGCTAATGCTATCGTTTAA

Protein sequence

>MS.gene23437.t1

MSKRGRGGSAGNKFRMSLGLPVAATVNCADNTGAKNLYIISVKGIKGRLNRLPSACVGDMVMATVKKGKPDLRKKVLPAVIVRQRKPWRRKDGVFMYFEDNAGVIVNPKGEMKGSAITGPIGKECADLWPRIASAANAIV