Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene96949.t1 | XP_004296407.1 | 100 | 140 | 0 | 0 | 1 | 140 | 1 | 140 | 5.40E-73 | 283.5 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene96949.t1 | P49690 | 98.6 | 140 | 2 | 0 | 1 | 140 | 1 | 140 | 6.4e-75 | 281.2 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene96949.t1 | M5WP13 | 100.0 | 140 | 0 | 0 | 1 | 140 | 1 | 140 | 3.9e-73 | 283.5 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene060429 | MS.gene96949 | 0.836801 | 7.56E-57 | -1.69E-46 |
| MS.gene061176 | MS.gene96949 | 0.809828 | 1.56E-50 | -1.69E-46 |
| MS.gene061177 | MS.gene96949 | 0.809782 | 1.60E-50 | -1.69E-46 |
| MS.gene061178 | MS.gene96949 | 0.809581 | 1.76E-50 | -1.69E-46 |
PPI:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene96949.t1 | MTR_7g098290 | 98.571 | 140 | 2 | 0 | 1 | 140 | 1 | 140 | 3.93e-98 | 278 |
| MS.gene96949.t1 | MTR_1g069585 | 98.571 | 140 | 2 | 0 | 1 | 140 | 1 | 140 | 3.93e-98 | 278 |
| MS.gene96949.t1 | MTR_7g098290 | 98.400 | 125 | 2 | 0 | 16 | 140 | 1 | 125 | 8.58e-87 | 249 |
| MS.gene96949.t1 | MTR_1g026090 | 81.481 | 135 | 10 | 1 | 2 | 136 | 42 | 161 | 3.07e-71 | 212 |
| MS.gene96949.t1 | MTR_5g054560 | 68.800 | 125 | 15 | 1 | 16 | 140 | 1 | 101 | 1.78e-53 | 164 |
| MS.gene96949.t1 | MTR_5g464620 | 89.796 | 49 | 5 | 0 | 14 | 62 | 16 | 64 | 3.73e-25 | 91.7 |
| MS.gene96949.t1 | MTR_2g070580 | 92.683 | 41 | 3 | 0 | 100 | 140 | 87 | 127 | 2.62e-21 | 83.2 |
| MS.gene96949.t1 | MTR_1779s0020 | 36.522 | 115 | 65 | 3 | 26 | 136 | 8 | 118 | 3.65e-16 | 70.1 |
| MS.gene96949.t1 | MTR_4g051390 | 36.522 | 115 | 65 | 3 | 26 | 136 | 8 | 118 | 4.98e-16 | 69.7 |
| MS.gene96949.t1 | MTR_2g087550 | 36.792 | 106 | 60 | 2 | 26 | 128 | 8 | 109 | 1.55e-14 | 67.8 |
| MS.gene96949.t1 | MTR_0002s0780 | 34.426 | 122 | 73 | 3 | 23 | 140 | 132 | 250 | 1.92e-14 | 68.2 |
| MS.gene96949.t1 | MTR_4g017930 | 80.556 | 36 | 5 | 1 | 104 | 139 | 13 | 46 | 1.09e-11 | 58.5 |
| MS.gene96949.t1 | MTR_4g066540 | 72.500 | 40 | 7 | 1 | 100 | 139 | 54 | 89 | 7.28e-11 | 55.5 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene96949.t1 | AT3G04400 | 98.571 | 140 | 2 | 0 | 1 | 140 | 1 | 140 | 1.65e-98 | 279 |
| MS.gene96949.t1 | AT2G33370 | 98.571 | 140 | 2 | 0 | 1 | 140 | 1 | 140 | 1.65e-98 | 279 |
| MS.gene96949.t1 | AT1G04480 | 98.571 | 140 | 2 | 0 | 1 | 140 | 1 | 140 | 1.65e-98 | 279 |
| MS.gene96949.t1 | AT3G04400 | 100.000 | 125 | 0 | 0 | 16 | 140 | 1 | 125 | 4.64e-88 | 252 |
| MS.gene96949.t1 | AT2G33370 | 100.000 | 125 | 0 | 0 | 16 | 140 | 1 | 125 | 4.64e-88 | 252 |
| MS.gene96949.t1 | ATCG00780 | 34.783 | 115 | 67 | 3 | 26 | 136 | 8 | 118 | 4.97e-15 | 67.0 |
Find 50 sgRNAs with CRISPR-Local
Find 74 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TTTACCTTTCATTTCTCCTT+TGG | 0.232028 | 7.4:-12072601 | None:intergenic |
| TTACCTTTCATTTCTCCTTT+GGG | 0.290146 | 7.4:-12072600 | None:intergenic |
| ATTCTTAGCTCCGGTGTTAT+CGG | 0.357276 | 7.4:-12072252 | None:intergenic |
| ACATGAAAACACCGTCCTTT+CGG | 0.371684 | 7.4:-12072433 | None:intergenic |
| GATCCGCGGGTAACAAGTTC+AGG | 0.386005 | 7.4:+12072189 | MS.gene96949:CDS |
| GGAAGGAGTGTGCTGATCTA+TGG | 0.394494 | 7.4:+12073027 | MS.gene96949:CDS |
| CCTTCCTTTGATACCTTTGA+CGG | 0.396996 | 7.4:-12072288 | None:intergenic |
| AGCACCTTCTTACGGAGATC+AGG | 0.422915 | 7.4:-12072374 | None:intergenic |
| TTCTGCTTGCGTTGGTGACA+TGG | 0.443666 | 7.4:+12072325 | MS.gene96949:CDS |
| CAATTCACCGTCGCCGCCAC+TGG | 0.459295 | 7.4:-12072227 | None:intergenic |
| TTAGCAGCACTTGCAATCCT+AGG | 0.471348 | 7.4:-12073050 | None:intergenic |
| CTCTACATCATTTCCGTCAA+AGG | 0.478393 | 7.4:+12072275 | MS.gene96949:CDS |
| GGTGATGGCCACCGTGAAGA+AGG | 0.480460 | 7.4:+12072346 | MS.gene96949:CDS |
| CGTTTGCCTTCTGCTTGCGT+TGG | 0.482632 | 7.4:+12072317 | MS.gene96949:CDS |
| TCTGCTATCACTGGTCCAAT+CGG | 0.485775 | 7.4:+12073005 | MS.gene96949:CDS |
| TAACAAGTTCAGGATGTCAC+TGG | 0.500497 | 7.4:+12072199 | MS.gene96949:CDS |
| CGGCAGGAAGCACCTTCTTA+CGG | 0.502055 | 7.4:-12072382 | None:intergenic |
| ATCAGGCTTACCCTTCTTCA+CGG | 0.504036 | 7.4:-12072357 | None:intergenic |
| CTCAGGTCGCGGAGGATCCG+CGG | 0.504873 | 7.4:+12072175 | MS.gene96949:intron |
| CTGCTATCACTGGTCCAATC+GGG | 0.511052 | 7.4:+12073006 | MS.gene96949:CDS |
| TTGTATTGTGCAGACAATGC+TGG | 0.531818 | 7.4:+12072564 | MS.gene96949:intron |
| ATTGTAGGGTCTGCTATCAC+TGG | 0.534637 | 7.4:+12072996 | MS.gene96949:intron |
| CAACGCAAGCAGAAGGCAAA+CGG | 0.538758 | 7.4:-12072316 | None:intergenic |
| TCAGCACACTCCTTCCCGAT+TGG | 0.539409 | 7.4:-12073020 | None:intergenic |
| CGCTGACGAACGATGACGGC+AGG | 0.540043 | 7.4:-12072398 | None:intergenic |
| ACACCGTCCTTTCGGCGCCA+TGG | 0.550488 | 7.4:-12072425 | None:intergenic |
| CCGTCAAAGGTATCAAAGGA+AGG | 0.551618 | 7.4:+12072288 | MS.gene96949:CDS |
| TCAGGTCGCGGAGGATCCGC+GGG | 0.560803 | 7.4:+12072176 | MS.gene96949:intron |
| AGTGTGCTGATCTATGGCCT+AGG | 0.561029 | 7.4:+12073033 | MS.gene96949:CDS |
| GGTGTTATTGTTAATCCCAA+AGG | 0.565013 | 7.4:+12072585 | MS.gene96949:CDS |
| TAAGCCTGATCTCCGTAAGA+AGG | 0.569085 | 7.4:+12072370 | MS.gene96949:CDS |
| ATTTCCGTCAAAGGTATCAA+AGG | 0.575410 | 7.4:+12072284 | MS.gene96949:CDS |
| GGGTCTACCAGTGGCGGCGA+CGG | 0.576990 | 7.4:+12072220 | MS.gene96949:CDS |
| GTGATGGCCACCGTGAAGAA+GGG | 0.579374 | 7.4:+12072347 | MS.gene96949:CDS |
| TTGCGTTGGTGACATGGTGA+TGG | 0.588839 | 7.4:+12072331 | MS.gene96949:CDS |
| GATGTAGAGATTCTTAGCTC+CGG | 0.596136 | 7.4:-12072261 | None:intergenic |
| AAACCATGGCGCCGAAAGGA+CGG | 0.604162 | 7.4:+12072422 | MS.gene96949:CDS |
| AACAAGTTCAGGATGTCACT+GGG | 0.607031 | 7.4:+12072200 | MS.gene96949:CDS |
| ATGTCACCAACGCAAGCAGA+AGG | 0.609870 | 7.4:-12072323 | None:intergenic |
| GCGTAAACCATGGCGCCGAA+AGG | 0.610086 | 7.4:+12072418 | MS.gene96949:CDS |
| GTCACTGGGTCTACCAGTGG+CGG | 0.621290 | 7.4:+12072214 | MS.gene96949:CDS |
| GTGAATTGCGCCGATAACAC+CGG | 0.624326 | 7.4:+12072242 | MS.gene96949:CDS |
| AATCCCAAAGGAGAAATGAA+AGG | 0.624552 | 7.4:+12072597 | MS.gene96949:CDS |
| TTTACGCTGACGAACGATGA+CGG | 0.626909 | 7.4:-12072402 | None:intergenic |
| GCAGCAGCAATGTCGAAGAG+AGG | 0.651240 | 7.4:+12072047 | None:intergenic |
| GATGTCACTGGGTCTACCAG+TGG | 0.653286 | 7.4:+12072211 | MS.gene96949:CDS |
| TCGTTCGTCAGCGTAAACCA+TGG | 0.671478 | 7.4:+12072408 | MS.gene96949:CDS |
| TATCACTGGTCCAATCGGGA+AGG | 0.675150 | 7.4:+12073010 | MS.gene96949:CDS |
| CATCCTGAACTTGTTACCCG+CGG | 0.683379 | 7.4:-12072192 | None:intergenic |
| AGGCTTACCCTTCTTCACGG+TGG | 0.718796 | 7.4:-12072354 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | TTAATAATCAGTATTAAAAT+TGG | + | chr7.4:12072874-12072893 | MS.gene96949:intron | 10.0% |
| !! | TAATCAGTATTAAAATTGGT+TGG | + | chr7.4:12072878-12072897 | MS.gene96949:intron | 20.0% |
| !! | TGATTCTTACTTAATATTTC+TGG | + | chr7.4:12072540-12072559 | MS.gene96949:intron | 20.0% |
| !!! | TAATTTTCATTTTGTTTCTC+AGG | + | chr7.4:12072158-12072177 | MS.gene96949:intron | 20.0% |
| !!! | TTATTAAAACGCAATTTTGT+AGG | - | chr7.4:12072141-12072160 | None:intergenic | 20.0% |
| ! | TGAAAATGAAAACGAAAATG+AGG | - | chr7.4:12072079-12072098 | None:intergenic | 25.0% |
| !! | AATGATTGGTGTTTAATAAG+TGG | + | chr7.4:12072706-12072725 | MS.gene96949:intron | 25.0% |
| ATGATAATATCGATGTAGGT+GGG | + | chr7.4:12072673-12072692 | MS.gene96949:intron | 30.0% | |
| ATTAAAATTGGTTGGAGGAT+CGG | + | chr7.4:12072886-12072905 | MS.gene96949:intron | 30.0% | |
| CACAACATCAAAACCAAAAA+CGG | - | chr7.4:12072938-12072957 | None:intergenic | 30.0% | |
| TCAGTATTAAAATTGGTTGG+AGG | + | chr7.4:12072881-12072900 | MS.gene96949:intron | 30.0% | |
| TGGGTAATCTATGTAATGAT+TGG | + | chr7.4:12072692-12072711 | MS.gene96949:intron | 30.0% | |
| TTACCTTTCATTTCTCCTTT+GGG | - | chr7.4:12072603-12072622 | None:intergenic | 30.0% | |
| TTTACCTTTCATTTCTCCTT+TGG | - | chr7.4:12072604-12072623 | None:intergenic | 30.0% | |
| !! | TATGTTTTTATCGCCGTTTT+TGG | + | chr7.4:12072922-12072941 | MS.gene96949:intron | 30.0% |
| !! | TCGTGTTGATTGTAATTGTA+GGG | + | chr7.4:12072982-12073001 | MS.gene96949:intron | 30.0% |
| !! | TTCGTGTTGATTGTAATTGT+AGG | + | chr7.4:12072981-12073000 | MS.gene96949:intron | 30.0% |
| AATCCCAAAGGAGAAATGAA+AGG | + | chr7.4:12072597-12072616 | MS.gene96949:CDS | 35.0% | |
| ACTGCTGTGTCTTCAATAAT+TGG | + | chr7.4:12072788-12072807 | MS.gene96949:intron | 35.0% | |
| CACGATGATAATATCGATGT+AGG | + | chr7.4:12072669-12072688 | MS.gene96949:intron | 35.0% | |
| CTACATCGATATTATCATCG+TGG | - | chr7.4:12072671-12072690 | None:intergenic | 35.0% | |
| GATGATAATATCGATGTAGG+TGG | + | chr7.4:12072672-12072691 | MS.gene96949:intron | 35.0% | |
| TTAAAATTGGTTGGAGGATC+GGG | + | chr7.4:12072887-12072906 | MS.gene96949:intron | 35.0% | |
| !! | ATTTCCGTCAAAGGTATCAA+AGG | + | chr7.4:12072284-12072303 | MS.gene96949:CDS | 35.0% |
| !! | GGTGTTATTGTTAATCCCAA+AGG | + | chr7.4:12072585-12072604 | MS.gene96949:CDS | 35.0% |
| !! | GGTTTTGATGTTGTGCTTTT+TGG | + | chr7.4:12072943-12072962 | MS.gene96949:intron | 35.0% |
| AACAAGTTCAGGATGTCACT+GGG | + | chr7.4:12072200-12072219 | MS.gene96949:CDS | 40.0% | |
| ACATGAAAACACCGTCCTTT+CGG | - | chr7.4:12072436-12072455 | None:intergenic | 40.0% | |
| CCTTCCTTTGATACCTTTGA+CGG | - | chr7.4:12072291-12072310 | None:intergenic | 40.0% | |
| CTCTACATCATTTCCGTCAA+AGG | + | chr7.4:12072275-12072294 | MS.gene96949:CDS | 40.0% | |
| GATGTAGAGATTCTTAGCTC+CGG | - | chr7.4:12072264-12072283 | None:intergenic | 40.0% | |
| TAACAAGTTCAGGATGTCAC+TGG | + | chr7.4:12072199-12072218 | MS.gene96949:CDS | 40.0% | |
| TTGTATTGTGCAGACAATGC+TGG | + | chr7.4:12072564-12072583 | MS.gene96949:intron | 40.0% | |
| ! | GGTGTTTTCATGTACTTCGA+AGG | + | chr7.4:12072443-12072462 | MS.gene96949:CDS | 40.0% |
| !! | ATTCTTAGCTCCGGTGTTAT+CGG | - | chr7.4:12072255-12072274 | None:intergenic | 40.0% |
| !! | TCATTTTGTTTCTCAGGTCG+CGG | + | chr7.4:12072164-12072183 | MS.gene96949:intron | 40.0% |
| ATCAGGCTTACCCTTCTTCA+CGG | - | chr7.4:12072360-12072379 | None:intergenic | 45.0% | |
| ATTGTAGGGTCTGCTATCAC+TGG | + | chr7.4:12072996-12073015 | MS.gene96949:intron | 45.0% | |
| TAAGCCTGATCTCCGTAAGA+AGG | + | chr7.4:12072370-12072389 | MS.gene96949:CDS | 45.0% | |
| TCTGCTATCACTGGTCCAAT+CGG | + | chr7.4:12073005-12073024 | MS.gene96949:CDS | 45.0% | |
| TTAGCAGCACTTGCAATCCT+AGG | - | chr7.4:12073053-12073072 | None:intergenic | 45.0% | |
| TTTACGCTGACGAACGATGA+CGG | - | chr7.4:12072405-12072424 | None:intergenic | 45.0% | |
| !! | CCGTCAAAGGTATCAAAGGA+AGG | + | chr7.4:12072288-12072307 | MS.gene96949:CDS | 45.0% |
| AGCACCTTCTTACGGAGATC+AGG | - | chr7.4:12072377-12072396 | None:intergenic | 50.0% | |
| ATGTCACCAACGCAAGCAGA+AGG | - | chr7.4:12072326-12072345 | None:intergenic | 50.0% | |
| CAACGCAAGCAGAAGGCAAA+CGG | - | chr7.4:12072319-12072338 | None:intergenic | 50.0% | |
| CTGCTATCACTGGTCCAATC+GGG | + | chr7.4:12073006-12073025 | MS.gene96949:CDS | 50.0% | |
| TATCACTGGTCCAATCGGGA+AGG | + | chr7.4:12073010-12073029 | MS.gene96949:CDS | 50.0% | |
| TCGTTCGTCAGCGTAAACCA+TGG | + | chr7.4:12072408-12072427 | MS.gene96949:CDS | 50.0% | |
| ! | AGTGTGCTGATCTATGGCCT+AGG | + | chr7.4:12073033-12073052 | MS.gene96949:CDS | 50.0% |
| ! | GGAAGGAGTGTGCTGATCTA+TGG | + | chr7.4:12073027-12073046 | MS.gene96949:CDS | 50.0% |
| ! | GTGAATTGCGCCGATAACAC+CGG | + | chr7.4:12072242-12072261 | MS.gene96949:CDS | 50.0% |
| !! | CATCCTGAACTTGTTACCCG+CGG | - | chr7.4:12072195-12072214 | None:intergenic | 50.0% |
| !! | TTCTGCTTGCGTTGGTGACA+TGG | + | chr7.4:12072325-12072344 | MS.gene96949:CDS | 50.0% |
| !! | TTGCGTTGGTGACATGGTGA+TGG | + | chr7.4:12072331-12072350 | MS.gene96949:CDS | 50.0% |
| !! | TTTTGTTTCTCAGGTCGCGG+AGG | + | chr7.4:12072167-12072186 | MS.gene96949:intron | 50.0% |
| AGGCTTACCCTTCTTCACGG+TGG | - | chr7.4:12072357-12072376 | None:intergenic | 55.0% | |
| CGTTTGCCTTCTGCTTGCGT+TGG | + | chr7.4:12072317-12072336 | MS.gene96949:CDS | 55.0% | |
| GATCCGCGGGTAACAAGTTC+AGG | + | chr7.4:12072189-12072208 | MS.gene96949:CDS | 55.0% | |
| GATGTCACTGGGTCTACCAG+TGG | + | chr7.4:12072211-12072230 | MS.gene96949:CDS | 55.0% | |
| GTGATGGCCACCGTGAAGAA+GGG | + | chr7.4:12072347-12072366 | MS.gene96949:CDS | 55.0% | |
| TCAGCACACTCCTTCCCGAT+TGG | - | chr7.4:12073023-12073042 | None:intergenic | 55.0% | |
| ! | AAACCATGGCGCCGAAAGGA+CGG | + | chr7.4:12072422-12072441 | MS.gene96949:CDS | 55.0% |
| !! | CGGCAGGAAGCACCTTCTTA+CGG | - | chr7.4:12072385-12072404 | None:intergenic | 55.0% |
| GTCACTGGGTCTACCAGTGG+CGG | + | chr7.4:12072214-12072233 | MS.gene96949:CDS | 60.0% | |
| ! | GCGTAAACCATGGCGCCGAA+AGG | + | chr7.4:12072418-12072437 | MS.gene96949:CDS | 60.0% |
| ! | GGTGATGGCCACCGTGAAGA+AGG | + | chr7.4:12072346-12072365 | MS.gene96949:CDS | 60.0% |
| ACACCGTCCTTTCGGCGCCA+TGG | - | chr7.4:12072428-12072447 | None:intergenic | 65.0% | |
| CAATTCACCGTCGCCGCCAC+TGG | - | chr7.4:12072230-12072249 | None:intergenic | 65.0% | |
| TGGAGGATCGGGCGAGTGTG+TGG | + | chr7.4:12072898-12072917 | MS.gene96949:intron | 65.0% | |
| ! | CGCTGACGAACGATGACGGC+AGG | - | chr7.4:12072401-12072420 | None:intergenic | 65.0% |
| CTCAGGTCGCGGAGGATCCG+CGG | + | chr7.4:12072175-12072194 | MS.gene96949:intron | 70.0% | |
| GGGTCTACCAGTGGCGGCGA+CGG | + | chr7.4:12072220-12072239 | MS.gene96949:CDS | 70.0% | |
| TCAGGTCGCGGAGGATCCGC+GGG | + | chr7.4:12072176-12072195 | MS.gene96949:intron | 70.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr7.4 | gene | 12072056 | 12073085 | 12072056 | ID=MS.gene96949 |
| chr7.4 | mRNA | 12072056 | 12073085 | 12072056 | ID=MS.gene96949.t1;Parent=MS.gene96949 |
| chr7.4 | exon | 12072056 | 12072068 | 12072056 | ID=MS.gene96949.t1.exon1;Parent=MS.gene96949.t1 |
| chr7.4 | CDS | 12072056 | 12072068 | 12072056 | ID=cds.MS.gene96949.t1;Parent=MS.gene96949.t1 |
| chr7.4 | exon | 12072180 | 12072464 | 12072180 | ID=MS.gene96949.t1.exon2;Parent=MS.gene96949.t1 |
| chr7.4 | CDS | 12072180 | 12072464 | 12072180 | ID=cds.MS.gene96949.t1;Parent=MS.gene96949.t1 |
| chr7.4 | exon | 12072577 | 12072618 | 12072577 | ID=MS.gene96949.t1.exon3;Parent=MS.gene96949.t1 |
| chr7.4 | CDS | 12072577 | 12072618 | 12072577 | ID=cds.MS.gene96949.t1;Parent=MS.gene96949.t1 |
| chr7.4 | exon | 12073003 | 12073085 | 12073003 | ID=MS.gene96949.t1.exon4;Parent=MS.gene96949.t1 |
| chr7.4 | CDS | 12073003 | 12073085 | 12073003 | ID=cds.MS.gene96949.t1;Parent=MS.gene96949.t1 |
| Gene Sequence |
| Protein sequence |