Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene54195.t1 | XP_024639027.1 | 91.2 | 160 | 8 | 1 | 1 | 154 | 1 | 160 | 8.30E-75 | 291.2 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene54195.t1 | Q7XC27 | 73.5 | 155 | 40 | 1 | 1 | 154 | 1 | 155 | 3.5e-58 | 227.3 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene54195.t1 | G7JYM3 | 88.3 | 154 | 7 | 1 | 1 | 154 | 1 | 143 | 2.1e-67 | 266.2 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049254 | MS.gene54195 | 0.815487 | 9.01E-52 | -1.69E-46 |
| MS.gene049350 | MS.gene54195 | 0.800337 | 1.51E-48 | -1.69E-46 |
| MS.gene049711 | MS.gene54195 | 0.823852 | 1.11E-53 | -1.69E-46 |
| MS.gene049977 | MS.gene54195 | 0.819918 | 9.02E-53 | -1.69E-46 |
| MS.gene050026 | MS.gene54195 | 0.818805 | 1.62E-52 | -1.69E-46 |
| MS.gene051247 | MS.gene54195 | 0.815992 | 6.96E-52 | -1.69E-46 |
| MS.gene051332 | MS.gene54195 | 0.808919 | 2.44E-50 | -1.69E-46 |
| MS.gene051594 | MS.gene54195 | 0.8084 | 3.16E-50 | -1.69E-46 |
| MS.gene051719 | MS.gene54195 | 0.820281 | 7.45E-53 | -1.69E-46 |
| MS.gene051720 | MS.gene54195 | 0.827712 | 1.34E-54 | -1.69E-46 |
| MS.gene05191 | MS.gene54195 | 0.82115 | 4.70E-53 | -1.69E-46 |
| MS.gene051957 | MS.gene54195 | 0.817085 | 3.96E-52 | -1.69E-46 |
| MS.gene052447 | MS.gene54195 | 0.813961 | 1.96E-51 | -1.69E-46 |
| MS.gene052463 | MS.gene54195 | 0.802786 | 4.75E-49 | -1.69E-46 |
| MS.gene052519 | MS.gene54195 | 0.824576 | 7.49E-54 | -1.69E-46 |
| MS.gene052534 | MS.gene54195 | 0.802357 | 5.83E-49 | -1.69E-46 |
| MS.gene052582 | MS.gene54195 | 0.81739 | 3.38E-52 | -1.69E-46 |
| MS.gene052584 | MS.gene54195 | 0.822148 | 2.77E-53 | -1.69E-46 |
| MS.gene052773 | MS.gene54195 | 0.800684 | 1.28E-48 | -1.69E-46 |
| MS.gene052829 | MS.gene54195 | 0.804496 | 2.10E-49 | -1.69E-46 |
| MS.gene053226 | MS.gene54195 | 0.81333 | 2.70E-51 | -1.69E-46 |
| MS.gene053396 | MS.gene54195 | 0.858505 | 7.87E-63 | -1.69E-46 |
| MS.gene053599 | MS.gene54195 | 0.801152 | 1.03E-48 | -1.69E-46 |
| MS.gene053883 | MS.gene54195 | 0.810445 | 1.15E-50 | -1.69E-46 |
| MS.gene053900 | MS.gene54195 | 0.802085 | 6.63E-49 | -1.69E-46 |
| MS.gene053974 | MS.gene54195 | 0.805621 | 1.22E-49 | -1.69E-46 |
| MS.gene05415 | MS.gene54195 | 0.812397 | 4.33E-51 | -1.69E-46 |
| MS.gene05422 | MS.gene54195 | 0.804188 | 2.44E-49 | -1.69E-46 |
| MS.gene05423 | MS.gene54195 | 0.841402 | 4.86E-58 | -1.69E-46 |
| MS.gene054651 | MS.gene54195 | 0.816059 | 6.72E-52 | -1.69E-46 |
| MS.gene054775 | MS.gene54195 | 0.807006 | 6.24E-50 | -1.69E-46 |
| MS.gene054991 | MS.gene54195 | 0.840922 | 6.49E-58 | -1.69E-46 |
| MS.gene05531 | MS.gene54195 | 0.826146 | 3.18E-54 | -1.69E-46 |
| MS.gene055465 | MS.gene54195 | 0.801955 | 7.05E-49 | -1.69E-46 |
| MS.gene055466 | MS.gene54195 | 0.808147 | 3.57E-50 | -1.69E-46 |
| MS.gene055778 | MS.gene54195 | 0.801537 | 8.59E-49 | -1.69E-46 |
| MS.gene055818 | MS.gene54195 | 0.806835 | 6.78E-50 | -1.69E-46 |
| MS.gene056050 | MS.gene54195 | 0.821753 | 3.41E-53 | -1.69E-46 |
| MS.gene05690 | MS.gene54195 | 0.805031 | 1.62E-49 | -1.69E-46 |
| MS.gene058037 | MS.gene54195 | 0.80978 | 1.60E-50 | -1.69E-46 |
| MS.gene058625 | MS.gene54195 | 0.842141 | 3.10E-58 | -1.69E-46 |
| MS.gene059015 | MS.gene54195 | 0.802192 | 6.30E-49 | -1.69E-46 |
| MS.gene059272 | MS.gene54195 | 0.834287 | 3.27E-56 | -1.69E-46 |
| MS.gene059460 | MS.gene54195 | 0.801872 | 7.33E-49 | -1.69E-46 |
| MS.gene059625 | MS.gene54195 | 0.837549 | 4.87E-57 | -1.69E-46 |
| MS.gene060134 | MS.gene54195 | 0.833359 | 5.58E-56 | -1.69E-46 |
| MS.gene060135 | MS.gene54195 | 0.806563 | 7.74E-50 | -1.69E-46 |
| MS.gene060519 | MS.gene54195 | 0.802922 | 4.46E-49 | -1.69E-46 |
| MS.gene060521 | MS.gene54195 | 0.830788 | 2.41E-55 | -1.69E-46 |
| MS.gene060634 | MS.gene54195 | -0.803468 | 3.44E-49 | -1.69E-46 |
| MS.gene061091 | MS.gene54195 | 0.805882 | 1.08E-49 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene54195.t1 | MTR_5g013560 | 88.312 | 154 | 7 | 1 | 1 | 154 | 1 | 143 | 6.43e-90 | 272 |
| MS.gene54195.t1 | MTR_4g099210 | 73.548 | 155 | 40 | 1 | 1 | 154 | 1 | 155 | 2.29e-73 | 230 |
| MS.gene54195.t1 | MTR_5g077900 | 61.905 | 147 | 56 | 0 | 279 | 425 | 50 | 196 | 9.17e-62 | 200 |
| MS.gene54195.t1 | MTR_7g046380 | 61.905 | 147 | 56 | 0 | 279 | 425 | 232 | 378 | 7.23e-61 | 204 |
| MS.gene54195.t1 | MTR_0002s1270 | 61.905 | 147 | 56 | 0 | 279 | 425 | 224 | 370 | 3.02e-60 | 202 |
| MS.gene54195.t1 | MTR_3g091440 | 59.877 | 162 | 63 | 1 | 1 | 160 | 1 | 162 | 4.33e-60 | 194 |
| MS.gene54195.t1 | MTR_3g091440 | 61.538 | 156 | 58 | 1 | 1 | 154 | 1 | 156 | 4.18e-58 | 191 |
| MS.gene54195.t1 | MTR_5g096420 | 62.857 | 140 | 52 | 0 | 15 | 154 | 31 | 170 | 1.73e-53 | 179 |
| MS.gene54195.t1 | MTR_8g099575 | 53.548 | 155 | 71 | 1 | 1 | 154 | 1 | 155 | 3.03e-53 | 177 |
| MS.gene54195.t1 | MTR_3g060730 | 62.143 | 140 | 53 | 0 | 15 | 154 | 31 | 170 | 6.44e-53 | 177 |
| MS.gene54195.t1 | MTR_4g134250 | 60.714 | 140 | 55 | 0 | 15 | 154 | 32 | 171 | 1.35e-52 | 176 |
| MS.gene54195.t1 | MTR_8g099570 | 55.844 | 154 | 66 | 1 | 1 | 154 | 46 | 197 | 1.97e-50 | 172 |
| MS.gene54195.t1 | MTR_1g016430 | 57.233 | 159 | 62 | 1 | 2 | 154 | 36 | 194 | 4.62e-50 | 171 |
| MS.gene54195.t1 | MTR_3g091440 | 60.448 | 134 | 53 | 0 | 21 | 154 | 24 | 157 | 2.05e-49 | 168 |
| MS.gene54195.t1 | MTR_3g060730 | 61.538 | 130 | 50 | 0 | 25 | 154 | 3 | 132 | 3.79e-48 | 164 |
| MS.gene54195.t1 | MTR_1g016430 | 61.719 | 128 | 49 | 0 | 27 | 154 | 1 | 128 | 6.52e-46 | 157 |
| MS.gene54195.t1 | MTR_1g016430 | 58.394 | 137 | 57 | 0 | 18 | 154 | 90 | 226 | 8.78e-45 | 158 |
| MS.gene54195.t1 | MTR_2g027500 | 52.143 | 140 | 67 | 0 | 15 | 154 | 29 | 168 | 2.63e-44 | 155 |
| MS.gene54195.t1 | MTR_2g027480 | 52.143 | 140 | 67 | 0 | 15 | 154 | 34 | 173 | 1.15e-43 | 153 |
| MS.gene54195.t1 | MTR_8g027620 | 52.143 | 140 | 67 | 0 | 15 | 154 | 39 | 178 | 1.67e-43 | 155 |
| MS.gene54195.t1 | MTR_2g027440 | 51.429 | 140 | 68 | 0 | 15 | 154 | 34 | 173 | 1.43e-42 | 150 |
| MS.gene54195.t1 | MTR_8g016170 | 50.345 | 145 | 72 | 0 | 10 | 154 | 37 | 181 | 2.59e-42 | 149 |
| MS.gene54195.t1 | MTR_8g016170 | 50.345 | 145 | 72 | 0 | 10 | 154 | 56 | 200 | 7.20e-42 | 149 |
| MS.gene54195.t1 | MTR_4g113505 | 53.488 | 129 | 60 | 0 | 26 | 154 | 6 | 134 | 1.45e-40 | 145 |
| MS.gene54195.t1 | MTR_2g027520 | 50.370 | 135 | 67 | 0 | 20 | 154 | 41 | 175 | 6.94e-40 | 143 |
| MS.gene54195.t1 | MTR_8g051620 | 89.831 | 59 | 6 | 0 | 279 | 337 | 50 | 108 | 2.55e-31 | 116 |
| MS.gene54195.t1 | MTR_8g051620 | 89.831 | 59 | 6 | 0 | 279 | 337 | 50 | 108 | 3.43e-31 | 116 |
| MS.gene54195.t1 | MTR_2g092960 | 40.881 | 159 | 88 | 3 | 269 | 425 | 1035 | 1189 | 6.38e-30 | 124 |
| MS.gene54195.t1 | MTR_0462s0020 | 36.986 | 146 | 86 | 2 | 280 | 423 | 122 | 263 | 2.94e-26 | 108 |
| MS.gene54195.t1 | MTR_8g017000 | 36.242 | 149 | 88 | 3 | 280 | 425 | 515 | 659 | 1.99e-23 | 103 |
| MS.gene54195.t1 | MTR_2g037270 | 36.242 | 149 | 88 | 3 | 280 | 425 | 515 | 659 | 2.54e-23 | 103 |
| MS.gene54195.t1 | MTR_3g011560 | 33.333 | 147 | 82 | 3 | 280 | 425 | 515 | 646 | 3.70e-18 | 87.8 |
| MS.gene54195.t1 | MTR_0057s0200 | 82.759 | 29 | 5 | 0 | 397 | 425 | 1 | 29 | 1.86e-11 | 59.7 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene54195.t1 | AT5G47100 | 68.485 | 165 | 51 | 1 | 1 | 164 | 1 | 165 | 3.34e-75 | 233 |
| MS.gene54195.t1 | AT5G47100 | 70.968 | 155 | 44 | 1 | 1 | 154 | 1 | 155 | 5.33e-73 | 229 |
| MS.gene54195.t1 | AT4G17615 | 69.032 | 155 | 47 | 1 | 1 | 154 | 1 | 155 | 2.03e-70 | 222 |
| MS.gene54195.t1 | AT4G17615 | 69.032 | 155 | 47 | 1 | 1 | 154 | 1 | 155 | 2.03e-70 | 222 |
| MS.gene54195.t1 | AT4G17615 | 69.032 | 155 | 47 | 1 | 1 | 154 | 1 | 155 | 2.03e-70 | 222 |
| MS.gene54195.t1 | AT4G17615 | 69.032 | 155 | 47 | 1 | 1 | 154 | 1 | 155 | 2.87e-70 | 223 |
| MS.gene54195.t1 | AT5G24270 | 57.862 | 159 | 62 | 1 | 1 | 154 | 1 | 159 | 7.79e-56 | 185 |
| MS.gene54195.t1 | AT5G24270 | 57.862 | 159 | 62 | 1 | 1 | 154 | 1 | 159 | 7.79e-56 | 185 |
| MS.gene54195.t1 | AT5G24270 | 57.862 | 159 | 62 | 1 | 1 | 154 | 1 | 159 | 7.79e-56 | 185 |
| MS.gene54195.t1 | AT4G26570 | 62.590 | 139 | 52 | 0 | 16 | 154 | 32 | 170 | 1.15e-52 | 177 |
| MS.gene54195.t1 | AT4G26570 | 60.839 | 143 | 52 | 1 | 16 | 154 | 32 | 174 | 1.53e-50 | 171 |
| MS.gene54195.t1 | AT5G24270 | 60.305 | 131 | 52 | 0 | 24 | 154 | 4 | 134 | 1.32e-49 | 168 |
| MS.gene54195.t1 | AT5G55990 | 59.712 | 139 | 56 | 0 | 16 | 154 | 32 | 170 | 4.56e-49 | 167 |
| MS.gene54195.t1 | AT5G55990 | 59.712 | 139 | 56 | 0 | 16 | 154 | 32 | 170 | 4.56e-49 | 167 |
| MS.gene54195.t1 | AT1G64480 | 53.061 | 147 | 69 | 0 | 15 | 161 | 20 | 166 | 8.21e-49 | 166 |
| MS.gene54195.t1 | AT1G64480 | 53.061 | 147 | 69 | 0 | 15 | 161 | 20 | 166 | 2.28e-48 | 165 |
| MS.gene54195.t1 | AT1G64480 | 53.061 | 147 | 69 | 0 | 15 | 161 | 20 | 166 | 2.28e-48 | 165 |
| MS.gene54195.t1 | AT1G64480 | 53.061 | 147 | 69 | 0 | 15 | 161 | 20 | 166 | 2.28e-48 | 165 |
| MS.gene54195.t1 | AT4G33000 | 58.824 | 136 | 56 | 0 | 20 | 155 | 57 | 192 | 4.41e-47 | 162 |
| MS.gene54195.t1 | AT4G33000 | 58.088 | 136 | 57 | 0 | 20 | 155 | 57 | 192 | 9.37e-47 | 160 |
| MS.gene54195.t1 | AT4G33000 | 58.088 | 136 | 57 | 0 | 20 | 155 | 57 | 192 | 9.37e-47 | 160 |
| MS.gene54195.t1 | AT4G33000 | 58.519 | 135 | 56 | 0 | 20 | 154 | 57 | 191 | 2.23e-45 | 158 |
| MS.gene54195.t1 | AT4G33000 | 58.519 | 135 | 56 | 0 | 20 | 154 | 67 | 201 | 2.41e-45 | 159 |
| MS.gene54195.t1 | AT4G17615 | 67.647 | 102 | 33 | 0 | 53 | 154 | 12 | 113 | 2.51e-42 | 148 |
| MS.gene54195.t1 | AT4G16350 | 51.773 | 141 | 64 | 1 | 14 | 154 | 28 | 164 | 1.59e-39 | 142 |
| MS.gene54195.t1 | AT4G16350 | 51.773 | 141 | 64 | 1 | 14 | 154 | 28 | 164 | 1.59e-39 | 142 |
| MS.gene54195.t1 | AT5G24270 | 57.547 | 106 | 45 | 0 | 49 | 154 | 7 | 112 | 1.27e-36 | 133 |
| MS.gene54195.t1 | AT4G01420 | 45.455 | 154 | 84 | 0 | 1 | 154 | 1 | 154 | 2.45e-35 | 130 |
| MS.gene54195.t1 | AT4G01420 | 45.455 | 154 | 84 | 0 | 1 | 154 | 1 | 154 | 3.96e-35 | 130 |
| MS.gene54195.t1 | AT4G26560 | 43.357 | 143 | 68 | 2 | 32 | 161 | 23 | 165 | 1.41e-29 | 115 |
| MS.gene54195.t1 | AT4G33000 | 60.000 | 90 | 36 | 0 | 20 | 109 | 57 | 146 | 1.09e-27 | 108 |
Find 73 sgRNAs with CRISPR-Local
Find 0 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TACAGTGAATGGATTGAATT+AGG | 0.142088 | 5.1:-6461899 | None:intergenic |
| GTTTCTGATGCAAGAATTAT+TGG | 0.187943 | 5.1:-6449991 | None:intergenic |
| TTGACTTCTTGTGGTGCATT+AGG | 0.224162 | 5.1:-6458560 | None:intergenic |
| TATGAAACTTAGGATTTATT+AGG | 0.242704 | 5.1:+6466766 | MS.gene54195:intron |
| TTACTTGGCACCGTTTCTTA+TGG | 0.263856 | 5.1:+6466193 | MS.gene54195:CDS |
| GATCGTATGTAGGATTTAAC+TGG | 0.292118 | 5.1:-6466143 | None:intergenic |
| GAGAAAGAGATTGAGGAGTT+AGG | 0.297620 | 5.1:-6461872 | None:intergenic |
| GAGAATTAGATTGAGGAGTT+AGG | 0.322185 | 5.1:-6461845 | None:intergenic |
| GGTTTGTTGTTATTTGTAGC+TGG | 0.322902 | 5.1:+6466823 | MS.gene54195:CDS |
| TGACTTCTTGTGGTGCATTA+GGG | 0.326113 | 5.1:-6458559 | None:intergenic |
| ACAAACAAATCAAATGTGTT+TGG | 0.350566 | 5.1:+6466065 | MS.gene54195:CDS |
| GTGAACGACGTTCCTTCTTC+AGG | 0.361046 | 5.1:+6462167 | MS.gene54195:CDS |
| CGGATTGATTTGTTGATTTG+TGG | 0.372650 | 5.1:-6461819 | None:intergenic |
| GTTTAATTTAGTTATGTCCT+TGG | 0.379523 | 5.1:+6462929 | MS.gene54195:intron |
| AGAAAGAGATTGAGGAGTTA+GGG | 0.386856 | 5.1:-6461871 | None:intergenic |
| TATCCACAATCAGTTGTGTT+AGG | 0.390652 | 5.1:-6466657 | None:intergenic |
| ATTATTGGGTCTTCCTGCTC+TGG | 0.393843 | 5.1:-6449976 | None:intergenic |
| ACAGTGAATGGATTGAATTA+GGG | 0.397206 | 5.1:-6461898 | None:intergenic |
| TTTCTGATGCAAGAATTATT+GGG | 0.398145 | 5.1:-6449990 | None:intergenic |
| TAGATTGAGGAGTTAGGGTT+CGG | 0.409822 | 5.1:-6461839 | None:intergenic |
| ACAACCAATGTATTATCCTT+TGG | 0.410346 | 5.1:-6466325 | None:intergenic |
| GTTTGTTGTTATTTGTAGCT+GGG | 0.420594 | 5.1:+6466824 | MS.gene54195:CDS |
| AGGAAGGGAGTAATTGACTT+TGG | 0.422183 | 5.1:+6458506 | MS.gene54195:CDS |
| GACCAATAACTTACAGTGAA+TGG | 0.422648 | 5.1:-6461910 | None:intergenic |
| TTGTTTCGAGGAAAAGGAAA+AGG | 0.432658 | 5.1:-6462095 | None:intergenic |
| AGAATTAGATTGAGGAGTTA+GGG | 0.437845 | 5.1:-6461844 | None:intergenic |
| CTCTATGATCTGGACAATAC+AGG | 0.438032 | 5.1:+6459025 | MS.gene54195:CDS |
| CAATCTATCTTGACTTCTTG+TGG | 0.438399 | 5.1:-6458569 | None:intergenic |
| CTTTCAGGAAGAATTTCAAT+TGG | 0.448198 | 5.1:+6457872 | MS.gene54195:intron |
| CAATGTTTAATTAGTACTGT+TGG | 0.456089 | 5.1:+6466802 | MS.gene54195:CDS |
| CTGCTAATATTACTACCTCT+TGG | 0.458064 | 5.1:+6466712 | MS.gene54195:CDS |
| AAAATGAAAGCGATATGAAC+AGG | 0.459408 | 5.1:-6461734 | None:intergenic |
| TTTGTAGCTGGGAGATATGT+TGG | 0.461985 | 5.1:+6466835 | MS.gene54195:CDS |
| GGTTTGTTGGCTTCGTCCTA+AGG | 0.478799 | 5.1:+6466533 | MS.gene54195:CDS |
| AGGGTTCGAGAAAGAGATTG+AGG | 0.480107 | 5.1:-6461879 | None:intergenic |
| GAAACTTAGGATTTATTAGG+AGG | 0.497449 | 5.1:+6466769 | MS.gene54195:intron |
| TCTATTTGATGTGAAGAGGA+AGG | 0.506361 | 5.1:+6458490 | MS.gene54195:CDS |
| AGGGTTCGAGAATTAGATTG+AGG | 0.511095 | 5.1:-6461852 | None:intergenic |
| AGGAAGAATATTTCCAGAGC+AGG | 0.512363 | 5.1:+6449963 | MS.gene54195:CDS |
| CAACCTAACACAACTGATTG+TGG | 0.514100 | 5.1:+6466654 | MS.gene54195:CDS |
| TCGGTCAACGGTGGTTCGTA+CGG | 0.514228 | 5.1:+6462142 | MS.gene54195:CDS |
| ATGGGTTGCTACCACTCTAA+AGG | 0.516160 | 5.1:+6449943 | MS.gene54195:CDS |
| TTTACGTGTATAGAGAGCAT+TGG | 0.518826 | 5.1:+6466284 | MS.gene54195:intron |
| ATCAAATACGTACCTGAAGA+AGG | 0.521934 | 5.1:-6462179 | None:intergenic |
| CCACCGTTGACCGATGAAGA+AGG | 0.523357 | 5.1:-6462133 | None:intergenic |
| AGTGGGTCGGTCATTGATGA+TGG | 0.525241 | 5.1:+6450337 | MS.gene54195:CDS |
| GGAGAATCTCTTCACAAGTC+GGG | 0.532057 | 5.1:+6457917 | MS.gene54195:CDS |
| AAGCAATACTGATTGAGCAT+TGG | 0.535473 | 5.1:+6462054 | MS.gene54195:intron |
| TATTACTACCTCTTGGATGA+AGG | 0.535487 | 5.1:+6466719 | MS.gene54195:CDS |
| GAGTCGAAATCCTTCTTCAT+CGG | 0.543128 | 5.1:+6462123 | MS.gene54195:CDS |
| ATTAATCGTCATTTGTCCAA+AGG | 0.543300 | 5.1:+6466309 | MS.gene54195:CDS |
| TTGTTCTATTTGATGTGAAG+AGG | 0.545682 | 5.1:+6458486 | MS.gene54195:CDS |
| TGTTTCGAGGAAAAGGAAAA+GGG | 0.548901 | 5.1:-6462094 | None:intergenic |
| TATACTTACTCCATAAGAAA+CGG | 0.550414 | 5.1:-6466203 | None:intergenic |
| AGATCTTGACACAACAATGA+AGG | 0.553286 | 5.1:+6466366 | MS.gene54195:CDS |
| CTATTTGATGTGAAGAGGAA+GGG | 0.557894 | 5.1:+6458491 | MS.gene54195:CDS |
| GAAATATTCTTCCTTTAGAG+TGG | 0.564614 | 5.1:-6449954 | None:intergenic |
| CTTCTTGTGGTGCATTAGGG+TGG | 0.574436 | 5.1:-6458556 | None:intergenic |
| AACTGGTTCTGTGTTCATTG+CGG | 0.576023 | 5.1:-6466126 | None:intergenic |
| AGGAGAATCTCTTCACAAGT+CGG | 0.578547 | 5.1:+6457916 | MS.gene54195:CDS |
| TATGAACAAAGTGTGTTACT+TGG | 0.580249 | 5.1:+6466178 | MS.gene54195:CDS |
| GATCGAAACAATTATTCAGA+AGG | 0.580669 | 5.1:+6459639 | MS.gene54195:CDS |
| TTGTCCAAAGGATAATACAT+TGG | 0.585455 | 5.1:+6466321 | MS.gene54195:CDS |
| ACGCAACTTAGATCGTATGT+AGG | 0.590800 | 5.1:-6466153 | None:intergenic |
| GATCTTTGTTAAATGAACAA+AGG | 0.606945 | 5.1:-6466348 | None:intergenic |
| AGGAAAAGGAAAAGGGCAGA+AGG | 0.620187 | 5.1:-6462087 | None:intergenic |
| TGTTTGTTTCAATACAGAGA+CGG | 0.620395 | 5.1:-6466049 | None:intergenic |
| GTCAACGGTGGTTCGTACGG+CGG | 0.622241 | 5.1:+6462145 | MS.gene54195:CDS |
| AATCCTTCTTCATCGGTCAA+CGG | 0.633811 | 5.1:+6462130 | MS.gene54195:CDS |
| TGATGATGGACTAATAAGCA+AGG | 0.636514 | 5.1:+6450351 | MS.gene54195:CDS |
| CCTTCTTCATCGGTCAACGG+TGG | 0.649294 | 5.1:+6462133 | MS.gene54195:CDS |
| ATGGTATACCTTCATCCAAG+AGG | 0.683941 | 5.1:-6466727 | None:intergenic |
| GTGCAATACTAGATACACCA+AGG | 0.728087 | 5.1:-6462946 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr5.1 | gene | 6449943 | 6466864 | 6449943 | ID=MS.gene54195 |
| chr5.1 | mRNA | 6449943 | 6466864 | 6449943 | ID=MS.gene54195.t1;Parent=MS.gene54195 |
| chr5.1 | exon | 6449943 | 6450018 | 6449943 | ID=MS.gene54195.t1.exon1;Parent=MS.gene54195.t1 |
| chr5.1 | CDS | 6449943 | 6450018 | 6449943 | ID=cds.MS.gene54195.t1;Parent=MS.gene54195.t1 |
| chr5.1 | exon | 6450290 | 6450372 | 6450290 | ID=MS.gene54195.t1.exon2;Parent=MS.gene54195.t1 |
| chr5.1 | CDS | 6450290 | 6450372 | 6450290 | ID=cds.MS.gene54195.t1;Parent=MS.gene54195.t1 |
| chr5.1 | exon | 6457879 | 6457938 | 6457879 | ID=MS.gene54195.t1.exon3;Parent=MS.gene54195.t1 |
| chr5.1 | CDS | 6457879 | 6457938 | 6457879 | ID=cds.MS.gene54195.t1;Parent=MS.gene54195.t1 |
| chr5.1 | exon | 6458482 | 6458590 | 6458482 | ID=MS.gene54195.t1.exon4;Parent=MS.gene54195.t1 |
| chr5.1 | CDS | 6458482 | 6458590 | 6458482 | ID=cds.MS.gene54195.t1;Parent=MS.gene54195.t1 |
| chr5.1 | exon | 6459014 | 6459066 | 6459014 | ID=MS.gene54195.t1.exon5;Parent=MS.gene54195.t1 |
| chr5.1 | CDS | 6459014 | 6459066 | 6459014 | ID=cds.MS.gene54195.t1;Parent=MS.gene54195.t1 |
| chr5.1 | exon | 6459580 | 6459660 | 6459580 | ID=MS.gene54195.t1.exon6;Parent=MS.gene54195.t1 |
| chr5.1 | CDS | 6459580 | 6459660 | 6459580 | ID=cds.MS.gene54195.t1;Parent=MS.gene54195.t1 |
| chr5.1 | exon | 6461711 | 6461918 | 6461711 | ID=MS.gene54195.t1.exon7;Parent=MS.gene54195.t1 |
| chr5.1 | CDS | 6461711 | 6461918 | 6461711 | ID=cds.MS.gene54195.t1;Parent=MS.gene54195.t1 |
| chr5.1 | exon | 6462057 | 6462188 | 6462057 | ID=MS.gene54195.t1.exon8;Parent=MS.gene54195.t1 |
| chr5.1 | CDS | 6462057 | 6462188 | 6462057 | ID=cds.MS.gene54195.t1;Parent=MS.gene54195.t1 |
| chr5.1 | exon | 6462940 | 6462970 | 6462940 | ID=MS.gene54195.t1.exon9;Parent=MS.gene54195.t1 |
| chr5.1 | CDS | 6462940 | 6462970 | 6462940 | ID=cds.MS.gene54195.t1;Parent=MS.gene54195.t1 |
| chr5.1 | exon | 6466040 | 6466216 | 6466040 | ID=MS.gene54195.t1.exon10;Parent=MS.gene54195.t1 |
| chr5.1 | CDS | 6466040 | 6466216 | 6466040 | ID=cds.MS.gene54195.t1;Parent=MS.gene54195.t1 |
| chr5.1 | exon | 6466297 | 6466401 | 6466297 | ID=MS.gene54195.t1.exon11;Parent=MS.gene54195.t1 |
| chr5.1 | CDS | 6466297 | 6466401 | 6466297 | ID=cds.MS.gene54195.t1;Parent=MS.gene54195.t1 |
| chr5.1 | exon | 6466491 | 6466554 | 6466491 | ID=MS.gene54195.t1.exon12;Parent=MS.gene54195.t1 |
| chr5.1 | CDS | 6466491 | 6466554 | 6466491 | ID=cds.MS.gene54195.t1;Parent=MS.gene54195.t1 |
| chr5.1 | exon | 6466645 | 6466740 | 6466645 | ID=MS.gene54195.t1.exon13;Parent=MS.gene54195.t1 |
| chr5.1 | CDS | 6466645 | 6466740 | 6466645 | ID=cds.MS.gene54195.t1;Parent=MS.gene54195.t1 |
| chr5.1 | exon | 6466778 | 6466864 | 6466778 | ID=MS.gene54195.t1.exon14;Parent=MS.gene54195.t1 |
| chr5.1 | CDS | 6466778 | 6466864 | 6466778 | ID=cds.MS.gene54195.t1;Parent=MS.gene54195.t1 |
| Gene Sequence |
| Protein sequence |
