Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene56253.t1 | XP_003598260.1 | 96 | 150 | 6 | 0 | 1 | 150 | 1 | 150 | 6.00E-74 | 288.1 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene56253.t1 | Q2HJI3 | 27.6 | 105 | 73 | 2 | 42 | 143 | 28 | 132 | 1.1e-07 | 59.3 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene56253.t1 | G7IYL4 | 96.0 | 150 | 6 | 0 | 1 | 150 | 1 | 150 | 4.3e-74 | 288.1 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene050114 | MS.gene56253 | 0.801245 | 9.86E-49 | -1.69E-46 |
| MS.gene050277 | MS.gene56253 | 0.815917 | 7.23E-52 | -1.69E-46 |
| MS.gene050303 | MS.gene56253 | 0.823259 | 1.53E-53 | -1.69E-46 |
| MS.gene050623 | MS.gene56253 | -0.807705 | 4.44E-50 | -1.69E-46 |
| MS.gene050824 | MS.gene56253 | 0.804786 | 1.83E-49 | -1.69E-46 |
| MS.gene051330 | MS.gene56253 | 0.804157 | 2.47E-49 | -1.69E-46 |
| MS.gene051678 | MS.gene56253 | 0.802224 | 6.21E-49 | -1.69E-46 |
| MS.gene05188 | MS.gene56253 | 0.822906 | 1.84E-53 | -1.69E-46 |
| MS.gene051903 | MS.gene56253 | 0.806374 | 8.49E-50 | -1.69E-46 |
| MS.gene052376 | MS.gene56253 | 0.817137 | 3.85E-52 | -1.69E-46 |
| MS.gene055603 | MS.gene56253 | 0.803491 | 3.40E-49 | -1.69E-46 |
| MS.gene055808 | MS.gene56253 | 0.835079 | 2.07E-56 | -1.69E-46 |
| MS.gene056091 | MS.gene56253 | 0.833723 | 4.53E-56 | -1.69E-46 |
| MS.gene056403 | MS.gene56253 | 0.868343 | 7.01E-66 | -1.69E-46 |
| MS.gene056605 | MS.gene56253 | 0.860248 | 2.36E-63 | -1.69E-46 |
| MS.gene056631 | MS.gene56253 | 0.823699 | 1.20E-53 | -1.69E-46 |
| MS.gene056986 | MS.gene56253 | 0.815451 | 9.18E-52 | -1.69E-46 |
| MS.gene057079 | MS.gene56253 | 0.806748 | 7.07E-50 | -1.69E-46 |
| MS.gene057140 | MS.gene56253 | 0.807221 | 5.62E-50 | -1.69E-46 |
| MS.gene058084 | MS.gene56253 | 0.800829 | 1.20E-48 | -1.69E-46 |
| MS.gene058238 | MS.gene56253 | 0.831296 | 1.81E-55 | -1.69E-46 |
| MS.gene058498 | MS.gene56253 | 0.832589 | 8.67E-56 | -1.69E-46 |
| MS.gene058500 | MS.gene56253 | 0.809026 | 2.32E-50 | -1.69E-46 |
| MS.gene058908 | MS.gene56253 | 0.820976 | 5.16E-53 | -1.69E-46 |
| MS.gene058911 | MS.gene56253 | 0.837874 | 4.02E-57 | -1.69E-46 |
| MS.gene059249 | MS.gene56253 | 0.808462 | 3.06E-50 | -1.69E-46 |
| MS.gene05954 | MS.gene56253 | 0.808857 | 2.52E-50 | -1.69E-46 |
| MS.gene059830 | MS.gene56253 | 0.822958 | 1.79E-53 | -1.69E-46 |
| MS.gene059935 | MS.gene56253 | 0.811924 | 5.49E-51 | -1.69E-46 |
| MS.gene06001 | MS.gene56253 | 0.827621 | 1.41E-54 | -1.69E-46 |
| MS.gene06011 | MS.gene56253 | 0.812493 | 4.12E-51 | -1.69E-46 |
| MS.gene060297 | MS.gene56253 | 0.826048 | 3.36E-54 | -1.69E-46 |
| MS.gene060429 | MS.gene56253 | 0.808665 | 2.77E-50 | -1.69E-46 |
| MS.gene060572 | MS.gene56253 | 0.81187 | 5.64E-51 | -1.69E-46 |
| MS.gene060586 | MS.gene56253 | 0.850564 | 1.57E-60 | -1.69E-46 |
| MS.gene060830 | MS.gene56253 | 0.84257 | 2.39E-58 | -1.69E-46 |
| MS.gene060907 | MS.gene56253 | 0.800759 | 1.24E-48 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene56253.t1 | MTR_3g009130 | 97.297 | 148 | 4 | 0 | 1 | 148 | 1 | 148 | 4.94e-96 | 283 |
| MS.gene56253.t1 | MTR_3g009150 | 95.683 | 139 | 6 | 0 | 246 | 384 | 66 | 204 | 9.70e-90 | 269 |
| MS.gene56253.t1 | MTR_3g009150 | 72.414 | 87 | 17 | 1 | 246 | 332 | 66 | 145 | 7.15e-35 | 126 |
| MS.gene56253.t1 | MTR_4g068280 | 37.594 | 133 | 79 | 3 | 251 | 381 | 81 | 211 | 1.54e-21 | 92.4 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene56253.t1 | AT2G31725 | 62.416 | 149 | 55 | 1 | 1 | 148 | 1 | 149 | 1.07e-67 | 211 |
| MS.gene56253.t1 | AT1G05730 | 60.403 | 149 | 58 | 1 | 1 | 148 | 1 | 149 | 7.24e-65 | 204 |
| MS.gene56253.t1 | AT2G43720 | 51.748 | 143 | 68 | 1 | 7 | 148 | 5 | 147 | 2.98e-52 | 171 |
| MS.gene56253.t1 | AT2G30695 | 58.209 | 134 | 56 | 0 | 250 | 383 | 63 | 196 | 5.05e-50 | 167 |
| MS.gene56253.t1 | AT2G30695 | 58.209 | 134 | 56 | 0 | 250 | 383 | 63 | 196 | 5.05e-50 | 167 |
| MS.gene56253.t1 | AT2G30695 | 58.209 | 134 | 56 | 0 | 250 | 383 | 63 | 196 | 5.05e-50 | 167 |
| MS.gene56253.t1 | AT1G05730 | 59.259 | 108 | 43 | 1 | 42 | 148 | 10 | 117 | 2.87e-42 | 144 |
| MS.gene56253.t1 | AT1G05740 | 45.652 | 138 | 62 | 2 | 1 | 125 | 1 | 138 | 1.03e-38 | 135 |
| MS.gene56253.t1 | AT2G43720 | 50.926 | 108 | 52 | 1 | 42 | 148 | 1 | 108 | 9.11e-37 | 129 |
| MS.gene56253.t1 | AT2G30695 | 51.724 | 58 | 28 | 0 | 250 | 307 | 63 | 120 | 2.90e-13 | 67.0 |
Find 70 sgRNAs with CRISPR-Local
Find 0 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CGATGATGTGTTTCAGAAAA+TGG | 0.139014 | 3.3:+4596874 | MS.gene56253:CDS |
| AAATGAGATGGCAAAGTTTC+AGG | 0.195239 | 3.3:+4586326 | MS.gene56253:CDS |
| GAAAAGAAATTGAAAGTAAA+AGG | 0.267734 | 3.3:+4591219 | MS.gene56253:CDS |
| TGGTGGGTGGTTACACAAAA+TGG | 0.275152 | 3.3:-4591148 | None:intergenic |
| ACCTCTAATAAAATGTCTTT+TGG | 0.275489 | 3.3:-4597713 | None:intergenic |
| CCAGGATTTCGTAGAGTAAA+AGG | 0.298327 | 3.3:+4596917 | MS.gene56253:CDS |
| CTCATATGCACATTTAAAAT+AGG | 0.309655 | 3.3:-4586214 | None:intergenic |
| GAAACGGCGGTGGCGGCGGT+TGG | 0.327124 | 3.3:+4590275 | MS.gene56253:CDS |
| CAGATAAGCATCGAGGTATC+TGG | 0.342280 | 3.3:+4596830 | MS.gene56253:intron |
| CGGCGGTGGCGGCGGTTGGG+TGG | 0.348394 | 3.3:+4590279 | MS.gene56253:CDS |
| AATTAGGTCAACAGAGAATT+AGG | 0.348786 | 3.3:+4585637 | MS.gene56253:CDS |
| GGGTGGAGGCTGTAGCTTGT+AGG | 0.357962 | 3.3:+4590296 | MS.gene56253:CDS |
| AGGCGAAACGGCGGTGGCGG+CGG | 0.358861 | 3.3:+4590271 | MS.gene56253:CDS |
| CGGTGGCGGCGGTTGGGTGG+AGG | 0.370957 | 3.3:+4590282 | MS.gene56253:CDS |
| AAACGGCGGTGGCGGCGGTT+GGG | 0.378225 | 3.3:+4590276 | MS.gene56253:CDS |
| GGAGGAAGAGAGGGCGATGA+TGG | 0.380669 | 3.3:+4590244 | MS.gene56253:CDS |
| GAGAGGCGAAACGGCGGTGG+CGG | 0.380708 | 3.3:+4590268 | MS.gene56253:CDS |
| ATGATTCTTTCTCGGTCGTT+TGG | 0.383140 | 3.3:-4591168 | None:intergenic |
| GCAGCAGCTGAAGCGCAATT+AGG | 0.389975 | 3.3:+4585621 | MS.gene56253:CDS |
| TAATTGACATGGTCTTGAAC+AGG | 0.399803 | 3.3:-4585705 | None:intergenic |
| GAGGAAGAGAGGGCGATGAT+GGG | 0.412907 | 3.3:+4590245 | MS.gene56253:CDS |
| TTCTTTCTCGGTCGTTTGGT+GGG | 0.433128 | 3.3:-4591164 | None:intergenic |
| ATTCTTTCTCGGTCGTTTGG+TGG | 0.442565 | 3.3:-4591165 | None:intergenic |
| TTCTAGGAAGAACTGTTGTC+TGG | 0.450400 | 3.3:-4589528 | None:intergenic |
| ATTGTGGAATGATTCTTTCT+CGG | 0.454348 | 3.3:-4591176 | None:intergenic |
| TCATAGCTCCAGGTTTCTGT+TGG | 0.458233 | 3.3:-4589413 | None:intergenic |
| AACAAGTTGAAAGCTTCCTT+TGG | 0.460762 | 3.3:+4589501 | MS.gene56253:CDS |
| GAAGTTGAATAGATCTATGA+TGG | 0.466964 | 3.3:+4589359 | MS.gene56253:CDS |
| GGGGAGAGGCGAAACGGCGG+TGG | 0.472999 | 3.3:+4590265 | MS.gene56253:CDS |
| AGATCTTGAAACTACATTTG+AGG | 0.477759 | 3.3:+4598695 | MS.gene56253:CDS |
| ATCATATCATTCATAGCTCC+AGG | 0.479620 | 3.3:-4589423 | None:intergenic |
| AGGAAGAGAGGGCGATGATG+GGG | 0.486664 | 3.3:+4590246 | MS.gene56253:CDS |
| CTCAACTGTGGCTGAGTATG+TGG | 0.495219 | 3.3:+4597784 | MS.gene56253:CDS |
| CGGCAGCTGCGAGATGATGA+TGG | 0.495337 | 3.3:+4590222 | MS.gene56253:CDS |
| TCAAAGCTCCAACAGAAACC+TGG | 0.503368 | 3.3:+4589405 | MS.gene56253:CDS |
| GATGATGGGGAGAGGCGAAA+CGG | 0.506595 | 3.3:+4590259 | MS.gene56253:CDS |
| GGTTTGTCAAGACAAGTATG+AGG | 0.511683 | 3.3:+4589380 | MS.gene56253:CDS |
| GACTGTTGCACATTAGAAAG+AGG | 0.512334 | 3.3:-4586297 | None:intergenic |
| GAGAGGGCGATGATGGGGAG+AGG | 0.517268 | 3.3:+4590251 | MS.gene56253:CDS |
| CCATGTCAATTATACTCTCC+AGG | 0.522500 | 3.3:+4585716 | MS.gene56253:CDS |
| TTGAATCGCTTCGTCAGCAC+AGG | 0.525639 | 3.3:-4589448 | None:intergenic |
| CTTTGATAGAAGCAGAAAGC+AGG | 0.531953 | 3.3:+4586239 | MS.gene56253:CDS |
| TGATGATGTTAGAGAACTAA+AGG | 0.538789 | 3.3:+4595968 | MS.gene56253:CDS |
| TATTAGCCACATGCAAGGAT+GGG | 0.541537 | 3.3:+4591275 | MS.gene56253:CDS |
| TTCAACTTGTTCGTAAGAAG+CGG | 0.544894 | 3.3:-4589489 | None:intergenic |
| ACTCTACGAAATCCTGGGAT+CGG | 0.550289 | 3.3:-4596911 | None:intergenic |
| CTTGAAACTACATTTGAGGA+AGG | 0.550589 | 3.3:+4598699 | MS.gene56253:CDS |
| TTTCTCGGTCGTTTGGTGGG+TGG | 0.553892 | 3.3:-4591161 | None:intergenic |
| ATATTAGCCACATGCAAGGA+TGG | 0.554606 | 3.3:+4591274 | MS.gene56253:CDS |
| GGCAGCTGCGAGATGATGAT+GGG | 0.567162 | 3.3:+4590223 | MS.gene56253:CDS |
| ATGATGATGGGAGGAAGAGA+GGG | 0.570217 | 3.3:+4590235 | MS.gene56253:CDS |
| GATGATGATGGGAGGAAGAG+AGG | 0.570863 | 3.3:+4590234 | MS.gene56253:CDS |
| GAGAATTAGGCAGAAACTTG+AGG | 0.580267 | 3.3:+4585650 | MS.gene56253:CDS |
| AAAGCTGCACAACCGATCCC+AGG | 0.580477 | 3.3:+4596899 | MS.gene56253:CDS |
| AATTGACATGGTCTTGAACA+GGG | 0.588865 | 3.3:-4585704 | None:intergenic |
| ATTAGCCACATGCAAGGATG+GGG | 0.592199 | 3.3:+4591276 | MS.gene56253:CDS |
| ACAGTCATTTGAAAATGAGA+TGG | 0.593900 | 3.3:+4586314 | MS.gene56253:CDS |
| CCTGGAGAGTATAATTGACA+TGG | 0.606570 | 3.3:-4585716 | None:intergenic |
| GATGGGGAGAGGCGAAACGG+CGG | 0.611539 | 3.3:+4590262 | MS.gene56253:CDS |
| ATTGACATGGTCTTGAACAG+GGG | 0.619900 | 3.3:-4585703 | None:intergenic |
| AGACATATTAGCCACATGCA+AGG | 0.627777 | 3.3:+4591270 | MS.gene56253:CDS |
| TGTGGCTGAGTATGTGGAGA+AGG | 0.628591 | 3.3:+4597790 | MS.gene56253:CDS |
| ACTGTTGTCTGGAATGCCAA+AGG | 0.633485 | 3.3:-4589517 | None:intergenic |
| GGAAATAATCAACTCAACTG+TGG | 0.634122 | 3.3:+4597772 | MS.gene56253:CDS |
| GTTTGTCAAGACAAGTATGA+GGG | 0.640452 | 3.3:+4589381 | MS.gene56253:CDS |
| AGCTGCGAGATGATGATGGG+AGG | 0.659347 | 3.3:+4590226 | MS.gene56253:CDS |
| TTCGATGGAATACTTTACGA+CGG | 0.664897 | 3.3:+4590202 | MS.gene56253:CDS |
| TTCTGTCCAGATAAGCATCG+AGG | 0.718719 | 3.3:+4596823 | MS.gene56253:intron |
| GGATTTCGTAGAGTAAAAGG+AGG | 0.718882 | 3.3:+4596920 | MS.gene56253:CDS |
| TATCTGGAAACAGAACACAG+AGG | 0.877907 | 3.3:+4596846 | MS.gene56253:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr3.3 | gene | 4585609 | 4598764 | 4585609 | ID=MS.gene56253 |
| chr3.3 | mRNA | 4585609 | 4598764 | 4585609 | ID=MS.gene56253.t1;Parent=MS.gene56253 |
| chr3.3 | exon | 4585609 | 4585737 | 4585609 | ID=MS.gene56253.t1.exon1;Parent=MS.gene56253.t1 |
| chr3.3 | CDS | 4585609 | 4585737 | 4585609 | ID=cds.MS.gene56253.t1;Parent=MS.gene56253.t1 |
| chr3.3 | exon | 4586210 | 4586347 | 4586210 | ID=MS.gene56253.t1.exon2;Parent=MS.gene56253.t1 |
| chr3.3 | CDS | 4586210 | 4586347 | 4586210 | ID=cds.MS.gene56253.t1;Parent=MS.gene56253.t1 |
| chr3.3 | exon | 4589357 | 4589537 | 4589357 | ID=MS.gene56253.t1.exon3;Parent=MS.gene56253.t1 |
| chr3.3 | CDS | 4589357 | 4589537 | 4589357 | ID=cds.MS.gene56253.t1;Parent=MS.gene56253.t1 |
| chr3.3 | exon | 4590200 | 4590317 | 4590200 | ID=MS.gene56253.t1.exon4;Parent=MS.gene56253.t1 |
| chr3.3 | CDS | 4590200 | 4590317 | 4590200 | ID=cds.MS.gene56253.t1;Parent=MS.gene56253.t1 |
| chr3.3 | exon | 4591128 | 4591297 | 4591128 | ID=MS.gene56253.t1.exon5;Parent=MS.gene56253.t1 |
| chr3.3 | CDS | 4591128 | 4591297 | 4591128 | ID=cds.MS.gene56253.t1;Parent=MS.gene56253.t1 |
| chr3.3 | exon | 4595913 | 4595989 | 4595913 | ID=MS.gene56253.t1.exon6;Parent=MS.gene56253.t1 |
| chr3.3 | CDS | 4595913 | 4595989 | 4595913 | ID=cds.MS.gene56253.t1;Parent=MS.gene56253.t1 |
| chr3.3 | exon | 4596833 | 4596941 | 4596833 | ID=MS.gene56253.t1.exon7;Parent=MS.gene56253.t1 |
| chr3.3 | CDS | 4596833 | 4596941 | 4596833 | ID=cds.MS.gene56253.t1;Parent=MS.gene56253.t1 |
| chr3.3 | exon | 4597346 | 4597359 | 4597346 | ID=MS.gene56253.t1.exon8;Parent=MS.gene56253.t1 |
| chr3.3 | CDS | 4597346 | 4597359 | 4597346 | ID=cds.MS.gene56253.t1;Parent=MS.gene56253.t1 |
| chr3.3 | exon | 4597710 | 4597811 | 4597710 | ID=MS.gene56253.t1.exon9;Parent=MS.gene56253.t1 |
| chr3.3 | CDS | 4597710 | 4597811 | 4597710 | ID=cds.MS.gene56253.t1;Parent=MS.gene56253.t1 |
| chr3.3 | exon | 4598648 | 4598764 | 4598648 | ID=MS.gene56253.t1.exon10;Parent=MS.gene56253.t1 |
| chr3.3 | CDS | 4598648 | 4598764 | 4598648 | ID=cds.MS.gene56253.t1;Parent=MS.gene56253.t1 |
| Gene Sequence |
| Protein sequence |
