AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene56300


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene56300.t1 MTR_3g010290 97.297 259 7 0 1 259 1 259 0.0 524
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene56300.t1 AT4G10170 53.077 260 95 5 1 259 1 234 2.34e-81 245
MS.gene56300.t1 AT4G10170 53.077 260 95 5 1 259 1 234 2.34e-81 245
MS.gene56300.t1 AT1G33475 43.561 264 117 7 1 260 1 236 1.71e-60 192
MS.gene56300.t1 AT1G33475 55.357 56 23 1 1 54 1 56 8.45e-16 73.2
MS.gene56300.t1 AT1G33475 43.956 91 39 2 171 260 55 134 2.38e-14 69.3

Find 61 sgRNAs with CRISPR-Local

Find 75 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTCTTCTTTGCAAAAGGATA+TGG 0.148205 3.1:-5411856 MS.gene56300:CDS
AAGGCCTGATTCAGGAAATT+TGG 0.252155 3.1:-5411910 MS.gene56300:CDS
TTCAACCAAAGCCCCTTTAT+TGG 0.253713 3.1:-5412033 MS.gene56300:CDS
TTTCACATAGTGGTAGTAAT+TGG 0.275380 3.1:-5412136 MS.gene56300:CDS
AATGAGAGATGTTGAGTTAG+AGG 0.307965 3.1:-5411961 MS.gene56300:CDS
TCAACCAAAGCCCCTTTATT+GGG 0.328565 3.1:-5412032 MS.gene56300:CDS
GACGAAACTTCCTCGACATT+TGG 0.372647 3.1:-5412110 MS.gene56300:CDS
ATTTCGAGACAATAGGTAAA+AGG 0.375196 3.1:-5412379 MS.gene56300:CDS
CCGTCCAAATTTCCTGAATC+AGG 0.383998 3.1:+5411906 None:intergenic
AGTTTACGTTCAAGAGAAAC+TGG 0.388560 3.1:-5412198 MS.gene56300:CDS
AGAGGATCAAGGCCTGATTC+AGG 0.392539 3.1:-5411918 MS.gene56300:CDS
ATTATTGTTGTGTTTCTAAA+GGG 0.397888 3.1:-5412496 MS.gene56300:CDS
ACGTAAACTGAATTCATGTT+TGG 0.402139 3.1:+5412212 None:intergenic
ATTTGTCCATTCAAATTACT+TGG 0.402162 3.1:+5412068 None:intergenic
TACATCTTCTTCTTTGCAAA+AGG 0.409637 3.1:-5411862 MS.gene56300:CDS
AGATGAAGGTCTTGGTAACT+TGG 0.412330 3.1:-5412309 MS.gene56300:CDS
TCTTCTTTGCAAAAGGATAT+GGG 0.415200 3.1:-5411855 MS.gene56300:CDS
TAGCTAGAACAATGCGTACT+TGG 0.417134 3.1:+5411781 None:intergenic
CATTATTGTTGTGTTTCTAA+AGG 0.425949 3.1:-5412497 MS.gene56300:CDS
CTCAAAACGTCCGTAAGAAA+TGG 0.447780 3.1:-5411809 MS.gene56300:CDS
CTCCACCACTATACGCATAC+AGG 0.453254 3.1:+5412465 None:intergenic
TACTTCACAATTGTAGATGA+AGG 0.455781 3.1:-5412323 MS.gene56300:CDS
CCATTCAAATTACTTGGTGA+TGG 0.462379 3.1:+5412074 None:intergenic
CACAGCCTTGTGCTTGGAGG+CGG 0.462743 3.1:-5412423 MS.gene56300:CDS
ACAATTGTAGATGAAGGTCT+TGG 0.466801 3.1:-5412317 MS.gene56300:CDS
ACAATAGGTAAAAGGACTTA+CGG 0.467377 3.1:-5412371 MS.gene56300:CDS
CTTGGCGCCACCATTTCTTA+CGG 0.469508 3.1:+5411799 None:intergenic
CAATAGGTAAAAGGACTTAC+GGG 0.479243 3.1:-5412370 MS.gene56300:CDS
TTAGAGAATGTTTCACATAG+TGG 0.480167 3.1:-5412146 MS.gene56300:CDS
AGGCGGTTCCTTCTTTCCAC+AGG 0.481926 3.1:-5412406 MS.gene56300:CDS
AAATTACTTGGTGATGGAGA+TGG 0.492575 3.1:+5412080 None:intergenic
TTCAGAAAGGTAGCTAGAAA+AGG 0.498079 3.1:-5412251 MS.gene56300:CDS
AGGTGGTATTTCGAGACAAT+AGG 0.503747 3.1:-5412386 MS.gene56300:CDS
GGTTCAATGAGGACGAGATC+AGG 0.506463 3.1:-5411834 MS.gene56300:CDS
AGGTAGCTAGAAAAGGTTCA+AGG 0.518283 3.1:-5412244 MS.gene56300:CDS
CACACCCTGTATGCGTATAG+TGG 0.522863 3.1:-5412470 MS.gene56300:CDS
CGGTTCCTTCTTTCCACAGG+TGG 0.523709 3.1:-5412403 MS.gene56300:CDS
CCATCACCAAGTAATTTGAA+TGG 0.529421 3.1:-5412074 MS.gene56300:CDS
AAATACCACCTGTGGAAAGA+AGG 0.529530 3.1:+5412398 None:intergenic
GTGATCAGATTACGAATGAC+AGG 0.539134 3.1:+5412173 None:intergenic
ACTTCCTCGACATTTGGCAT+TGG 0.540398 3.1:-5412104 MS.gene56300:CDS
GCAAGAGAAGAAGAAAGTAA+AGG 0.545957 3.1:-5411997 MS.gene56300:CDS
TGTCACAGCCTTGTGCTTGG+AGG 0.553859 3.1:-5412426 MS.gene56300:CDS
GAATGTCACAGCCTTGTGCT+TGG 0.555477 3.1:-5412429 MS.gene56300:CDS
AATCTACAGATAGAGGATCA+AGG 0.570567 3.1:-5411929 MS.gene56300:CDS
CACTATACGCATACAGGGTG+TGG 0.574835 3.1:+5412471 None:intergenic
GGTAGCTAGAAAAGGTTCAA+GGG 0.580926 3.1:-5412243 MS.gene56300:CDS
AAAACGTCCGTAAGAAATGG+TGG 0.598377 3.1:-5411806 MS.gene56300:CDS
TTGTCTCGAAATACCACCTG+TGG 0.605879 3.1:+5412390 None:intergenic
AGGAACCGCCTCCAAGCACA+AGG 0.610638 3.1:+5412418 None:intergenic
GTAGCTAGAAAAGGTTCAAG+GGG 0.613162 3.1:-5412242 MS.gene56300:CDS
AAGATGTAGCAGCACCACCT+TGG 0.620183 3.1:+5411877 None:intergenic
AATTTGGACGGTATTAGCCA+AGG 0.623716 3.1:-5411894 MS.gene56300:CDS
TGTCAGAGATGAATTCAGAA+AGG 0.624051 3.1:-5412264 MS.gene56300:CDS
CCTGATTCAGGAAATTTGGA+CGG 0.627032 3.1:-5411906 MS.gene56300:CDS
TCCACCACTATACGCATACA+GGG 0.629092 3.1:+5412466 None:intergenic
GTAAATGATCAGATCAGGCA+CGG 0.633105 3.1:+5411625 None:intergenic
ACCCTGTATGCGTATAGTGG+TGG 0.642922 3.1:-5412467 MS.gene56300:CDS
TGGACCAATGCCAAATGTCG+AGG 0.656618 3.1:+5412100 None:intergenic
TTGGACGGTATTAGCCAAGG+TGG 0.682644 3.1:-5411891 MS.gene56300:CDS
AAAAGGATATGGGTTCAATG+AGG 0.721400 3.1:-5411845 MS.gene56300:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! GTATAATATTATTTGTCATC+TGG - chr3.1:5412416-5412435 MS.gene56300:CDS 20.0%
!!! ATTATTGTTGTGTTTCTAAA+GGG - chr3.1:5411663-5411682 MS.gene56300:intron 20.0%
! AATACGATAACGATCAAATT+CGG + chr3.1:5412499-5412518 None:intergenic 25.0%
! ATTTGTCCATTCAAATTACT+TGG + chr3.1:5412094-5412113 None:intergenic 25.0%
! CATAAAAAATCTACAGATAG+AGG - chr3.1:5412223-5412242 MS.gene56300:CDS 25.0%
!! CATTATTGTTGTGTTTCTAA+AGG - chr3.1:5411662-5411681 MS.gene56300:intron 25.0%
!! TACTAGATTTTCCCAATAAA+GGG + chr3.1:5412141-5412160 None:intergenic 25.0%
!! TTACTAGATTTTCCCAATAA+AGG + chr3.1:5412142-5412161 None:intergenic 25.0%
ACAATAGGTAAAAGGACTTA+CGG - chr3.1:5411788-5411807 MS.gene56300:CDS 30.0%
ACGTAAACTGAATTCATGTT+TGG + chr3.1:5411950-5411969 None:intergenic 30.0%
ATTTCGAGACAATAGGTAAA+AGG - chr3.1:5411780-5411799 MS.gene56300:CDS 30.0%
GAATTTGATCGTTATCGTAT+TGG - chr3.1:5412498-5412517 MS.gene56300:CDS 30.0%
TACATCTTCTTCTTTGCAAA+AGG - chr3.1:5412297-5412316 MS.gene56300:CDS 30.0%
TACTTCACAATTGTAGATGA+AGG - chr3.1:5411836-5411855 MS.gene56300:CDS 30.0%
TCTTCTTTGCAAAAGGATAT+GGG - chr3.1:5412304-5412323 MS.gene56300:CDS 30.0%
TTAGAGAATGTTTCACATAG+TGG - chr3.1:5412013-5412032 MS.gene56300:CDS 30.0%
TTCTTCTTTGCAAAAGGATA+TGG - chr3.1:5412303-5412322 MS.gene56300:CDS 30.0%
TTTCACATAGTGGTAGTAAT+TGG - chr3.1:5412023-5412042 MS.gene56300:CDS 30.0%
! ACTAGATTTTCCCAATAAAG+GGG + chr3.1:5412140-5412159 None:intergenic 30.0%
AAAAGGATATGGGTTCAATG+AGG - chr3.1:5412314-5412333 MS.gene56300:CDS 35.0%
AATCTACAGATAGAGGATCA+AGG - chr3.1:5412230-5412249 MS.gene56300:CDS 35.0%
AATGAGAGATGTTGAGTTAG+AGG - chr3.1:5412198-5412217 MS.gene56300:CDS 35.0%
ACAATTGTAGATGAAGGTCT+TGG - chr3.1:5411842-5411861 MS.gene56300:CDS 35.0%
AGTTTACGTTCAAGAGAAAC+TGG - chr3.1:5411961-5411980 MS.gene56300:CDS 35.0%
ATCGAATGCATGATATACCA+CGG + chr3.1:5412453-5412472 None:intergenic 35.0%
CAATAGGTAAAAGGACTTAC+GGG - chr3.1:5411789-5411808 MS.gene56300:CDS 35.0%
CCATCACCAAGTAATTTGAA+TGG - chr3.1:5412085-5412104 MS.gene56300:CDS 35.0%
GCAAGAGAAGAAGAAAGTAA+AGG - chr3.1:5412162-5412181 MS.gene56300:CDS 35.0%
TGTCAGAGATGAATTCAGAA+AGG - chr3.1:5411895-5411914 MS.gene56300:CDS 35.0%
TTCAGAAAGGTAGCTAGAAA+AGG - chr3.1:5411908-5411927 MS.gene56300:CDS 35.0%
! ATTTCTTACGGACGTTTTGA+GGG + chr3.1:5412351-5412370 None:intergenic 35.0%
! CCATTCAAATTACTTGGTGA+TGG + chr3.1:5412088-5412107 None:intergenic 35.0%
!! AAATTACTTGGTGATGGAGA+TGG + chr3.1:5412082-5412101 None:intergenic 35.0%
!! CTTGGTAGTTCTTCAGTTTT+TGG - chr3.1:5411868-5411887 MS.gene56300:CDS 35.0%
AAAACGTCCGTAAGAAATGG+TGG - chr3.1:5412353-5412372 MS.gene56300:CDS 40.0%
AAATACCACCTGTGGAAAGA+AGG + chr3.1:5411764-5411783 None:intergenic 40.0%
AAGGCCTGATTCAGGAAATT+TGG - chr3.1:5412249-5412268 MS.gene56300:CDS 40.0%
AATTTGGACGGTATTAGCCA+AGG - chr3.1:5412265-5412284 MS.gene56300:CDS 40.0%
AGGTAGCTAGAAAAGGTTCA+AGG - chr3.1:5411915-5411934 MS.gene56300:CDS 40.0%
AGGTGGTATTTCGAGACAAT+AGG - chr3.1:5411773-5411792 MS.gene56300:CDS 40.0%
ATCTGGTTAGTAATATGCCG+TGG - chr3.1:5412433-5412452 MS.gene56300:CDS 40.0%
CCTGATTCAGGAAATTTGGA+CGG - chr3.1:5412253-5412272 MS.gene56300:CDS 40.0%
CTCAAAACGTCCGTAAGAAA+TGG - chr3.1:5412350-5412369 MS.gene56300:CDS 40.0%
GGTAGCTAGAAAAGGTTCAA+GGG - chr3.1:5411916-5411935 MS.gene56300:CDS 40.0%
GTAGCTAGAAAAGGTTCAAG+GGG - chr3.1:5411917-5411936 MS.gene56300:CDS 40.0%
GTGATCAGATTACGAATGAC+AGG + chr3.1:5411989-5412008 None:intergenic 40.0%
TAGCTAGAACAATGCGTACT+TGG + chr3.1:5412381-5412400 None:intergenic 40.0%
TCAACCAAAGCCCCTTTATT+GGG - chr3.1:5412127-5412146 MS.gene56300:CDS 40.0%
TTCAACCAAAGCCCCTTTAT+TGG - chr3.1:5412126-5412145 MS.gene56300:CDS 40.0%
! AGATGAAGGTCTTGGTAACT+TGG - chr3.1:5411850-5411869 MS.gene56300:CDS 40.0%
! CATTTCTTACGGACGTTTTG+AGG + chr3.1:5412352-5412371 None:intergenic 40.0%
! TTTTCCCAATAAAGGGGCTT+TGG + chr3.1:5412134-5412153 None:intergenic 40.0%
!! AAGGACTTACGGGTTTTTAG+TGG - chr3.1:5411799-5411818 MS.gene56300:CDS 40.0%
!!! TACGGGTTTTTAGTGGAAGA+TGG - chr3.1:5411806-5411825 MS.gene56300:CDS 40.0%
ACTTCCTCGACATTTGGCAT+TGG - chr3.1:5412055-5412074 MS.gene56300:CDS 45.0%
CCGTCCAAATTTCCTGAATC+AGG + chr3.1:5412256-5412275 None:intergenic 45.0%
GACGAAACTTCCTCGACATT+TGG - chr3.1:5412049-5412068 MS.gene56300:CDS 45.0%
TCCACCACTATACGCATACA+GGG + chr3.1:5411696-5411715 None:intergenic 45.0%
TTGTCTCGAAATACCACCTG+TGG + chr3.1:5411772-5411791 None:intergenic 45.0%
AAGATGTAGCAGCACCACCT+TGG + chr3.1:5412285-5412304 None:intergenic 50.0%
ACCCTGTATGCGTATAGTGG+TGG - chr3.1:5411692-5411711 MS.gene56300:intron 50.0%
AGAGGATCAAGGCCTGATTC+AGG - chr3.1:5412241-5412260 MS.gene56300:CDS 50.0%
CACACCCTGTATGCGTATAG+TGG - chr3.1:5411689-5411708 MS.gene56300:intron 50.0%
CACTATACGCATACAGGGTG+TGG + chr3.1:5411691-5411710 None:intergenic 50.0%
CTCCACCACTATACGCATAC+AGG + chr3.1:5411697-5411716 None:intergenic 50.0%
CTTGGCGCCACCATTTCTTA+CGG + chr3.1:5412363-5412382 None:intergenic 50.0%
GGTTCAATGAGGACGAGATC+AGG - chr3.1:5412325-5412344 MS.gene56300:CDS 50.0%
TGGACCAATGCCAAATGTCG+AGG + chr3.1:5412062-5412081 None:intergenic 50.0%
TTGGACGGTATTAGCCAAGG+TGG - chr3.1:5412268-5412287 MS.gene56300:CDS 50.0%
! GAATGTCACAGCCTTGTGCT+TGG - chr3.1:5411730-5411749 MS.gene56300:intron 50.0%
AGGCGGTTCCTTCTTTCCAC+AGG - chr3.1:5411753-5411772 MS.gene56300:intron 55.0%
CGGTTCCTTCTTTCCACAGG+TGG - chr3.1:5411756-5411775 MS.gene56300:intron 55.0%
! TGTCACAGCCTTGTGCTTGG+AGG - chr3.1:5411733-5411752 MS.gene56300:intron 55.0%
AGGAACCGCCTCCAAGCACA+AGG + chr3.1:5411744-5411763 None:intergenic 60.0%
! CACAGCCTTGTGCTTGGAGG+CGG - chr3.1:5411736-5411755 MS.gene56300:intron 60.0%


Chromosome Type Strat End Strand Name
chr3.1 gene 5411638 5412543 5411638 ID=MS.gene56300
chr3.1 mRNA 5411638 5412543 5411638 ID=MS.gene56300.t1;Parent=MS.gene56300
chr3.1 exon 5411770 5412543 5411770 ID=MS.gene56300.t1.exon1;Parent=MS.gene56300.t1
chr3.1 CDS 5411770 5412543 5411770 ID=cds.MS.gene56300.t1;Parent=MS.gene56300.t1
chr3.1 exon 5411638 5411652 5411638 ID=MS.gene56300.t1.exon2;Parent=MS.gene56300.t1
chr3.1 CDS 5411638 5411652 5411638 ID=cds.MS.gene56300.t1;Parent=MS.gene56300.t1
Gene Sequence

>MS.gene56300

ATGGATTCAATTCAAAATAAAGTTCATTATTGTTGTGTTTCTAAAGGGAGCCACACCCTGTATGCGTATAGTGGTGGAGATAAAGAAGTTGAGAATGTCACAGCCTTGTGCTTGGAGGCGGTTCCTTCTTTCCACAGGTGGTATTTCGAGACAATAGGTAAAAGGACTTACGGGTTTTTAGTGGAAGATGGATATGTTTACTTCACAATTGTAGATGAAGGTCTTGGTAACTTGGTAGTTCTTCAGTTTTTGGAACATGTCAGAGATGAATTCAGAAAGGTAGCTAGAAAAGGTTCAAGGGGATTTTCGCCAAACATGAATTCAGTTTACGTTCAAGAGAAACTGGTTCCTGTCATTCGTAATCTGATCACTTCATTAGAGAATGTTTCACATAGTGGTAGTAATTGGAGAGACGAAACTTCCTCGACATTTGGCATTGGTCCATCTCCATCACCAAGTAATTTGAATGGACAAATTGAAAATGTTGTTTCAACCAAAGCCCCTTTATTGGGAAAATCTAGTAAGCAAGAGAAGAAGAAAGTAAAGGATCATGTGATTGCAATGAGAGATGTTGAGTTAGAGGAGCATAAAAAATCTACAGATAGAGGATCAAGGCCTGATTCAGGAAATTTGGACGGTATTAGCCAAGGTGGTGCTGCTACATCTTCTTCTTTGCAAAAGGATATGGGTTCAATGAGGACGAGATCAGGCCCTCAAAACGTCCGTAAGAAATGGTGGCGCCAAGTACGCATTGTTCTAGCTATCGATGCAGCTGTATGTATAATATTATTTGTCATCTGGTTAGTAATATGCCGTGGTATATCATGCATTCGATAGAACTCAATCATCTATGTGACTCCGAATTTGATCGTTATCGTATTGGAAGTCTAGATTATCCGTGCCTGA

Protein sequence

>MS.gene56300.t1

MDSIQNKVHYCCVSKGSHTLYAYSGGDKEVENVTALCLEAVPSFHRWYFETIGKRTYGFLVEDGYVYFTIVDEGLGNLVVLQFLEHVRDEFRKVARKGSRGFSPNMNSVYVQEKLVPVIRNLITSLENVSHSGSNWRDETSSTFGIGPSPSPSNLNGQIENVVSTKAPLLGKSSKQEKKKVKDHVIAMRDVELEEHKKSTDRGSRPDSGNLDGISQGGAATSSSLQKDMGSMRTRSGPQNVRKKWWRQVRIVLAIDAAIIRA