AlfalfaGEDB | MS.gene59645

M. sativa cultivar XinJiangDaYe / MS.gene59645

KEGG: -
GO: GO:0000166 GO:0003674 GO:0003824 GO:0004672 GO:0005488 GO:0005524 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0008144 GO:0008150 GO:0008152 GO:0009987 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0017076 GO:0019538 GO:0030554 GO:0032553 GO:0032555 GO:0032559 GO:0035639 GO:0036094 GO:0036211 GO:0043167 GO:0043168 GO:0043170 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0071704 GO:0097159 GO:0097367 GO:0140096 GO:1901265 GO:1901363 GO:1901564
PFAM: PF00069.28

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene59645.t1	AES91544.2	96.4	55	2	0	1	55	1	55	2.30E-19	104

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene59645.t1	Q9LDI3	74.5	55	14	0	1	55	1	55	2.6e-16	85.1

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene59645.t1	A0A072USP3	96.4	55	2	0	1	55	1	55	1.6e-19	104.0

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene049142	MS.gene59645	0.818683	1.72E-52	-1.69E-46
MS.gene049254	MS.gene59645	0.807927	3.98E-50	-1.69E-46
MS.gene049368	MS.gene59645	0.809027	2.32E-50	-1.69E-46
MS.gene049499	MS.gene59645	0.814445	1.54E-51	-1.69E-46
MS.gene049585	MS.gene59645	0.801916	7.18E-49	-1.69E-46
MS.gene049983	MS.gene59645	0.806987	6.30E-50	-1.69E-46
MS.gene050414	MS.gene59645	0.804009	2.65E-49	-1.69E-46
MS.gene051279	MS.gene59645	0.81339	2.62E-51	-1.69E-46
MS.gene051744	MS.gene59645	0.804235	2.38E-49	-1.69E-46
MS.gene051800	MS.gene59645	0.818665	1.74E-52	-1.69E-46
MS.gene052037	MS.gene59645	0.807887	4.06E-50	-1.69E-46
MS.gene052307	MS.gene59645	0.830798	2.40E-55	-1.69E-46
MS.gene052463	MS.gene59645	0.828943	6.79E-55	-1.69E-46
MS.gene052493	MS.gene59645	0.805729	1.16E-49	-1.69E-46
MS.gene052609	MS.gene59645	0.831597	1.53E-55	-1.69E-46
MS.gene052674	MS.gene59645	0.804667	1.93E-49	-1.69E-46
MS.gene052675	MS.gene59645	0.80651	7.94E-50	-1.69E-46
MS.gene052677	MS.gene59645	0.808303	3.31E-50	-1.69E-46
MS.gene05350	MS.gene59645	0.817181	3.77E-52	-1.69E-46
MS.gene053620	MS.gene59645	0.805957	1.04E-49	-1.69E-46
MS.gene05423	MS.gene59645	0.814545	1.46E-51	-1.69E-46
MS.gene054479	MS.gene59645	0.83487	2.33E-56	-1.69E-46
MS.gene054576	MS.gene59645	0.824488	7.85E-54	-1.69E-46
MS.gene054892	MS.gene59645	0.824759	6.78E-54	-1.69E-46
MS.gene054893	MS.gene59645	0.822443	2.36E-53	-1.69E-46
MS.gene055141	MS.gene59645	0.801807	7.56E-49	-1.69E-46
MS.gene055231	MS.gene59645	0.807989	3.86E-50	-1.69E-46
MS.gene055327	MS.gene59645	0.808599	2.86E-50	-1.69E-46
MS.gene055864	MS.gene59645	0.817336	3.48E-52	-1.69E-46
MS.gene056174	MS.gene59645	0.813879	2.05E-51	-1.69E-46
MS.gene056308	MS.gene59645	0.801558	8.51E-49	-1.69E-46
MS.gene056507	MS.gene59645	0.812073	5.09E-51	-1.69E-46
MS.gene056699	MS.gene59645	0.800143	1.66E-48	-1.69E-46
MS.gene05686	MS.gene59645	0.822154	2.76E-53	-1.69E-46
MS.gene058081	MS.gene59645	0.837558	4.84E-57	-1.69E-46
MS.gene058289	MS.gene59645	0.830752	2.46E-55	-1.69E-46
MS.gene058429	MS.gene59645	0.814374	1.59E-51	-1.69E-46
MS.gene059170	MS.gene59645	0.811829	5.76E-51	-1.69E-46
MS.gene059438	MS.gene59645	0.8103	1.23E-50	-1.69E-46
MS.gene059439	MS.gene59645	0.840853	6.77E-58	-1.69E-46
MS.gene059774	MS.gene59645	0.815346	9.69E-52	-1.69E-46
MS.gene059965	MS.gene59645	0.815139	1.08E-51	-1.69E-46
MS.gene060167	MS.gene59645	0.80217	6.37E-49	-1.69E-46
MS.gene060259	MS.gene59645	0.800762	1.24E-48	-1.69E-46
MS.gene060373	MS.gene59645	0.832034	1.19E-55	-1.69E-46
MS.gene060380	MS.gene59645	0.806234	9.08E-50	-1.69E-46
MS.gene06052	MS.gene59645	0.834933	2.25E-56	-1.69E-46
MS.gene060697	MS.gene59645	0.814613	1.41E-51	-1.69E-46
MS.gene060777	MS.gene59645	0.813822	2.11E-51	-1.69E-46
MS.gene060785	MS.gene59645	0.846717	1.82E-59	-1.69E-46
MS.gene06089	MS.gene59645	0.809266	2.06E-50	-1.69E-46
MS.gene060932	MS.gene59645	0.832639	8.43E-56	-1.69E-46
MS.gene060933	MS.gene59645	0.831264	1.84E-55	-1.69E-46
MS.gene060937	MS.gene59645	0.81236	4.41E-51	-1.69E-46
MS.gene061100	MS.gene59645	0.816996	4.14E-52	-1.69E-46
MS.gene06121	MS.gene59645	0.803065	4.16E-49	-1.69E-46

PPI:

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene59645.t1	MTR_4g114670	96.364	55	2	1	55	1	55	3.29e-30	108
MS.gene59645.t1	MTR_4g114670	96.364	55	2	1	55	1	55	5.77e-30	108
MS.gene59645.t1	MTR_4g114670	96.364	55	2	1	55	1	55	6.55e-30	108
MS.gene59645.t1	MTR_5g067150	71.154	52	15	4	55	2	53	1.22e-21	85.1
MS.gene59645.t1	MTR_5g067150	71.154	52	15	4	55	2	53	1.65e-21	85.1
MS.gene59645.t1	MTR_5g067150	71.154	52	15	4	55	2	53	2.36e-21	84.7
MS.gene59645.t1	MTR_5g088350	66.038	53	18	3	55	5	57	2.19e-20	81.6
MS.gene59645.t1	MTR_5g088350	64.815	54	19	2	55	4	57	3.62e-20	81.6
MS.gene59645.t1	MTR_5g088350	66.038	53	18	3	55	5	57	4.84e-20	81.3
MS.gene59645.t1	MTR_2g049790	66.667	51	17	5	55	17	67	3.34e-18	75.9
MS.gene59645.t1	MTR_8g024600	56.863	51	22	5	55	11	61	5.28e-15	67.0
MS.gene59645.t1	MTR_8g024590	56.863	51	22	5	55	11	61	7.22e-15	66.6
MS.gene59645.t1	MTR_4g128820	56.000	50	22	6	55	5	54	1.13e-12	60.5

Query id	Subject id	identity %	alignment length	mismatches	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene59645.t1	AT5G35410	74.545	55	14	1	55	1	55	4.97e-23	89.7
MS.gene59645.t1	AT5G35410	74.545	55	14	1	55	1	55	1.50e-22	87.8
MS.gene59645.t1	AT2G26980	66.667	54	18	2	55	5	58	9.72e-22	84.3
MS.gene59645.t1	AT2G26980	66.667	54	18	2	55	5	58	1.69e-20	82.4
MS.gene59645.t1	AT2G26980	66.667	54	18	2	55	5	58	2.28e-20	82.0
MS.gene59645.t1	AT4G24400	71.154	52	15	4	55	2	53	7.96e-20	80.5
MS.gene59645.t1	AT2G26980	66.667	54	18	2	55	5	58	8.13e-20	80.5
MS.gene59645.t1	AT4G24400	71.154	52	15	4	55	2	53	9.56e-20	80.5
MS.gene59645.t1	AT2G26980	66.667	54	18	2	55	15	68	1.00e-19	80.5
MS.gene59645.t1	AT5G21326	61.111	54	21	2	55	4	57	9.59e-18	74.7
MS.gene59645.t1	AT1G30270	62.500	48	18	8	55	28	75	4.08e-16	70.1
MS.gene59645.t1	AT1G30270	62.500	48	18	8	55	28	75	4.15e-16	70.1
MS.gene59645.t1	AT1G01140	54.902	51	23	5	55	13	63	8.00e-16	69.3
MS.gene59645.t1	AT1G01140	54.902	51	23	5	55	13	63	8.07e-16	69.3
MS.gene59645.t1	AT1G01140	54.902	51	23	5	55	13	63	8.25e-16	69.3
MS.gene59645.t1	AT5G21222	56.604	53	23	3	55	5	57	4.18e-14	64.3
MS.gene59645.t1	AT5G21222	56.604	53	23	3	55	5	57	4.18e-14	64.3
MS.gene59645.t1	AT5G21222	56.604	53	23	3	55	5	57	4.18e-14	64.3
MS.gene59645.t1	AT5G21222	56.604	53	23	3	55	5	57	4.18e-14	64.3
MS.gene59645.t1	AT5G01810	60.870	46	18	10	55	11	56	2.80e-11	56.6
MS.gene59645.t1	AT5G01810	60.870	46	18	10	55	11	56	3.12e-11	56.2
MS.gene59645.t1	AT5G01810	60.870	46	18	10	55	11	56	3.12e-11	56.2
MS.gene59645.t1	AT3G17510	58.537	41	17	7	47	16	56	4.62e-11	55.8

Find 14 sgRNAs with CRISPR-Local

Find 12 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAGAAAGTGAGGAGAAAGAT+TGG	0.376571	8.1:+11874502	MS.gene59645:CDS
TATGAAGTTGGTAGAACAAT+TGG	0.383751	8.1:+11874529	MS.gene59645:CDS
CAAACACAGAATGGTTGAAC+AGG	0.443401	8.1:+11874642	MS.gene59645:CDS
AAGATTGGAAAGTATGAAGT+TGG	0.456314	8.1:+11874517	MS.gene59645:CDS
TTTGCAAAACACACCGAGAC+AGG	0.459686	8.1:+11874577	MS.gene59645:CDS
AACCATTCTGTGTTTGAGAA+TGG	0.473451	8.1:-11874635	None:intergenic
CGCGACACTTTCCCCTGTCT+CGG	0.504061	8.1:-11874590	None:intergenic
TACCATTCTCAAACACAGAA+TGG	0.543262	8.1:+11874633	MS.gene59645:CDS
TCAAAATAATGAAGAAAGTG+AGG	0.554812	8.1:+11874491	None:intergenic
TTGCAAAACACACCGAGACA+GGG	0.585182	8.1:+11874578	MS.gene59645:CDS
AAGTGTCGCGATTAAAGTCA+TGG	0.590091	8.1:+11874603	MS.gene59645:CDS
TGGTGAAGGTACATTCGCTA+AGG	0.613664	8.1:+11874549	MS.gene59645:CDS
GTTGGTAGAACAATTGGTGA+AGG	0.621321	8.1:+11874535	MS.gene59645:CDS
TGCAAAACACACCGAGACAG+GGG	0.649155	8.1:+11874579	MS.gene59645:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!	AAGATTGGAAAGTATGAAGT+TGG	+	chr8.1:11874517-11874536	MS.gene59645:CDS	30.0%
!!	TATGAAGTTGGTAGAACAAT+TGG	+	chr8.1:11874529-11874548	MS.gene59645:CDS	30.0%
	AACCATTCTGTGTTTGAGAA+TGG	-	chr8.1:11874638-11874657	None:intergenic	35.0%
	AAGAAAGTGAGGAGAAAGAT+TGG	+	chr8.1:11874502-11874521	MS.gene59645:CDS	35.0%
	TACCATTCTCAAACACAGAA+TGG	+	chr8.1:11874633-11874652	MS.gene59645:CDS	35.0%
!	AAGTGTCGCGATTAAAGTCA+TGG	+	chr8.1:11874603-11874622	MS.gene59645:CDS	40.0%
!	GTTGGTAGAACAATTGGTGA+AGG	+	chr8.1:11874535-11874554	MS.gene59645:CDS	40.0%
	TTGCAAAACACACCGAGACA+GGG	+	chr8.1:11874578-11874597	MS.gene59645:CDS	45.0%
	TTTGCAAAACACACCGAGAC+AGG	+	chr8.1:11874577-11874596	MS.gene59645:CDS	45.0%
!	TGGTGAAGGTACATTCGCTA+AGG	+	chr8.1:11874549-11874568	MS.gene59645:CDS	45.0%
	TGCAAAACACACCGAGACAG+GGG	+	chr8.1:11874579-11874598	MS.gene59645:CDS	50.0%
	CGCGACACTTTCCCCTGTCT+CGG	-	chr8.1:11874593-11874612	None:intergenic	60.0%

Chromosome	Type	Strat	End	Strand	Name
chr8.1	gene	11874499	11874663	11874499	ID=MS.gene59645
chr8.1	mRNA	11874499	11874663	11874499	ID=MS.gene59645.t1;Parent=MS.gene59645
chr8.1	exon	11874499	11874663	11874499	ID=MS.gene59645.t1.exon1;Parent=MS.gene59645.t1
chr8.1	CDS	11874499	11874663	11874499	ID=cds.MS.gene59645.t1;Parent=MS.gene59645.t1

Gene Sequence	>MS.gene59645 ATGAAGAAAGTGAGGAGAAAGATTGGAAAGTATGAAGTTGGTAGAACAATTGGTGAAGGTACATTCGCTAAGGTCAAATTTGCAAAACACACCGAGACAGGGGAAAGTGTCGCGATTAAAGTCATGGCTAAAACTACCATTCTCAAACACAGAATGGTTGAACAG
Protein sequence	>MS.gene59645.t1 MKKVRRKIGKYEVGRTIGEGTFAKVKFAKHTETGESVAIKVMAKTTILKHRMVEQ