AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene60124


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene60124.t1 MTR_8g039090 95.312 192 9 0 1 192 1 192 5.43e-136 378
MS.gene60124.t1 MTR_3g019160 76.316 190 45 0 3 192 2 191 1.40e-108 308
MS.gene60124.t1 MTR_3g019090 71.579 190 54 0 3 192 2 191 2.76e-104 298
MS.gene60124.t1 MTR_3g019150 72.632 190 51 1 3 192 2 190 2.33e-101 290
MS.gene60124.t1 MTR_3g019120 62.304 191 69 1 3 190 2 192 6.79e-85 256
MS.gene60124.t1 MTR_3g019120 62.304 191 69 1 3 190 2 192 1.17e-83 256
MS.gene60124.t1 MTR_3g019090 71.831 142 40 0 51 192 1 142 8.30e-78 229
MS.gene60124.t1 MTR_3g019120 65.132 152 53 0 39 190 1 152 4.32e-71 223
MS.gene60124.t1 MTR_3g019150 75.000 100 25 0 93 192 35 134 1.21e-53 167
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene60124.t1 AT3G27890 74.866 187 47 0 5 191 10 196 1.26e-101 291

Find 43 sgRNAs with CRISPR-Local

Find 97 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ATCAAAGTGGCAGCTCTTTC+TGG 0.186880 8.4:+59202674 MS.gene60124:CDS
CCATCATCATTGAACTTTGC+AGG 0.314182 8.4:-59203952 None:intergenic
CGTCTAGACCACCAAATGTT+TGG 0.318819 8.4:+59203788 MS.gene60124:CDS
CATTGAAGAATGCAATTGAT+TGG 0.367565 8.4:+59203764 MS.gene60124:CDS
GTCTAGACCACCAAATGTTT+GGG 0.381599 8.4:+59203789 MS.gene60124:CDS
GCTTTGCCAGCCCAAACATT+TGG 0.411118 8.4:-59203799 None:intergenic
CAATCAATTGCATTCTTCAA+TGG 0.420729 8.4:-59203763 None:intergenic
GCATTTACCCTAAGGCTTCA+AGG 0.432027 8.4:+59204033 MS.gene60124:CDS
ATTGAAGAATGCAATTGATT+GGG 0.454057 8.4:+59203765 MS.gene60124:CDS
TTGCCAGCCCAAACATTTGG+TGG 0.456284 8.4:-59203796 None:intergenic
ATAAGCACTGGAGGAGACTT+TGG 0.465806 8.4:+59203832 MS.gene60124:CDS
GCACTGGAGGAGACTTTGGC+GGG 0.469020 8.4:+59203836 MS.gene60124:CDS
AAGACGAACCAGCACGGATA+AGG 0.470569 8.4:-59202727 None:intergenic
CTTTGCAGGCATTTACCCTA+AGG 0.478396 8.4:+59204025 MS.gene60124:CDS
CATTCAGGCCTTATCCGTGC+TGG 0.509003 8.4:+59202719 MS.gene60124:CDS
CAACACTGATCTTGAAAACA+AGG 0.510617 8.4:+59202907 MS.gene60124:CDS
CCTGCAAAGTTCAATGATGA+TGG 0.528301 8.4:+59203952 MS.gene60124:CDS
GCAGCTGCAATCATAAGCAC+TGG 0.542454 8.4:+59203820 MS.gene60124:CDS
TTTGATTGATGAAGGGGCTA+AGG 0.544643 8.4:+59203978 MS.gene60124:CDS
GCAGCAATTGAGTTGAGTAA+AGG 0.545033 8.4:+59202830 MS.gene60124:intron
TTGAGTAAAGGAGCAATTGA+AGG 0.546127 8.4:+59202842 MS.gene60124:CDS
CAGCACGGATAAGGCCTGAA+TGG 0.546637 8.4:-59202718 None:intergenic
AGACCACCAAATGTTTGGGC+TGG 0.554510 8.4:+59203793 MS.gene60124:CDS
GATGGCGATTTGATTGATGA+AGG 0.564435 8.4:+59203970 MS.gene60124:CDS
GAGTATAACTACTCTGTCAC+AGG 0.571097 8.4:+59203004 MS.gene60124:CDS
CACTATCATCTACGCCAAGT+TGG 0.576435 8.4:+59203868 MS.gene60124:CDS
CGAAAAGCTTCAACAAGAGG+TGG 0.582016 8.4:-59202938 None:intergenic
TCAAGATCAGTGTTGTACAT+TGG 0.583062 8.4:-59202899 None:intergenic
GATCAATATAAATTCCAACT+TGG 0.584290 8.4:-59203882 None:intergenic
AGCACTGGAGGAGACTTTGG+CGG 0.584571 8.4:+59203835 MS.gene60124:CDS
TGGCGAAAAGCTTCAACAAG+AGG 0.594853 8.4:-59202941 None:intergenic
ATGGCGATTTGATTGATGAA+GGG 0.608092 8.4:+59203971 MS.gene60124:CDS
CAAAAGAAGACGAACCAGCA+CGG 0.622016 8.4:-59202733 None:intergenic
ACTGGAGGAGACTTTGGCGG+GGG 0.627120 8.4:+59203838 MS.gene60124:CDS
AATGCTCTTAGAAAGAACTC+AGG 0.630349 8.4:-59203922 None:intergenic
TCATCATTGAACTTTGCAGG+TGG 0.635212 8.4:-59203949 None:intergenic
GTGACAGAGTAGTTATACTC+AGG 0.639364 8.4:-59203001 None:intergenic
TGGCGATTTGATTGATGAAG+GGG 0.641939 8.4:+59203972 MS.gene60124:CDS
CACTGGAGGAGACTTTGGCG+GGG 0.645839 8.4:+59203837 MS.gene60124:CDS
GCTGCAATCATAAGCACTGG+AGG 0.650272 8.4:+59203823 MS.gene60124:CDS
TATCAGCTTGAAGAATCTTG+TGG 0.652286 8.4:-59202961 None:intergenic
AACACTGATCTTGAAAACAA+GGG 0.673165 8.4:+59202908 MS.gene60124:CDS
GGCAGCAGCAGTGATCAAAG+TGG 0.706403 8.4:+59202661 MS.gene60124:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TTTGTAATTAAAAAATTGAT+AGG - chr8.4:59203664-59203683 None:intergenic 10.0%
!! TATTATATAAATAGTTGCAA+AGG - chr8.4:59203176-59203195 None:intergenic 15.0%
!! AAAAAATTGATAGGTAGAAA+TGG - chr8.4:59203655-59203674 None:intergenic 20.0%
! AACCCAAAATATTGCAATTA+TGG - chr8.4:59203304-59203323 None:intergenic 25.0%
! ATATAAATAGTTGCAAAGGT+AGG - chr8.4:59203172-59203191 None:intergenic 25.0%
! ATGACATATTTGATTGCAAA+TGG + chr8.4:59203257-59203276 MS.gene60124:intron 25.0%
! ATTGAAGAATGCAATTGATT+GGG + chr8.4:59203765-59203784 MS.gene60124:CDS 25.0%
! GATCAATATAAATTCCAACT+TGG - chr8.4:59203885-59203904 None:intergenic 25.0%
! TATAATACTATGATTCCTCT+TGG + chr8.4:59203189-59203208 MS.gene60124:intron 25.0%
! TGACACTATCAACATTTAAA+GGG + chr8.4:59203334-59203353 MS.gene60124:intron 25.0%
! TTGACACTATCAACATTTAA+AGG + chr8.4:59203333-59203352 MS.gene60124:intron 25.0%
! TTTGCAATCAAATATGTCAT+TGG - chr8.4:59203257-59203276 None:intergenic 25.0%
!! TCTTTTCTTCTTCTATTACA+AGG - chr8.4:59203084-59203103 None:intergenic 25.0%
AACACTGATCTTGAAAACAA+GGG + chr8.4:59202908-59202927 MS.gene60124:CDS 30.0%
ACTATATCTCCGAATTTAGA+AGG - chr8.4:59203590-59203609 None:intergenic 30.0%
AGAAAAACTTCATACCATTC+AGG + chr8.4:59202704-59202723 MS.gene60124:CDS 30.0%
ATTGCAAAAACGATTGCATA+TGG + chr8.4:59203487-59203506 MS.gene60124:intron 30.0%
CAATCAATTGCATTCTTCAA+TGG - chr8.4:59203766-59203785 None:intergenic 30.0%
CATTGAAGAATGCAATTGAT+TGG + chr8.4:59203764-59203783 MS.gene60124:CDS 30.0%
CTATATCTCCGAATTTAGAA+GGG - chr8.4:59203589-59203608 None:intergenic 30.0%
! AATGGTATGAAGTTTTTCTG+AGG - chr8.4:59202703-59202722 None:intergenic 30.0%
! AGGCCATAATTGCAATATTT+TGG + chr8.4:59203298-59203317 MS.gene60124:intron 30.0%
! ATGGTATGAAGTTTTTCTGA+GGG - chr8.4:59202702-59202721 None:intergenic 30.0%
! CGATTTTTGTTCAAGAAGTT+CGG + chr8.4:59203131-59203150 MS.gene60124:intron 30.0%
! GGCCATAATTGCAATATTTT+GGG + chr8.4:59203299-59203318 MS.gene60124:intron 30.0%
! GTTGTAGTATTGAATTTGCA+TGG + chr8.4:59202795-59202814 MS.gene60124:intron 30.0%
!! ATATGCAATCGTTTTTGCAA+TGG - chr8.4:59203488-59203507 None:intergenic 30.0%
!! TATGCAATCGTTTTTGCAAT+GGG - chr8.4:59203487-59203506 None:intergenic 30.0%
AATGCTCTTAGAAAGAACTC+AGG - chr8.4:59203925-59203944 None:intergenic 35.0%
ATGGCGATTTGATTGATGAA+GGG + chr8.4:59203971-59203990 MS.gene60124:CDS 35.0%
CAACACTGATCTTGAAAACA+AGG + chr8.4:59202907-59202926 MS.gene60124:CDS 35.0%
CACTAAGTCTGTGTTTGATA+AGG - chr8.4:59203630-59203649 None:intergenic 35.0%
TATCAGCTTGAAGAATCTTG+TGG - chr8.4:59202964-59202983 None:intergenic 35.0%
TCAAGATCAGTGTTGTACAT+TGG - chr8.4:59202902-59202921 None:intergenic 35.0%
TGGAGCTAAACACAAAACAA+AGG - chr8.4:59203746-59203765 None:intergenic 35.0%
TTGAGTAAAGGAGCAATTGA+AGG + chr8.4:59202842-59202861 MS.gene60124:CDS 35.0%
! AAATATGTCATTGGTACCGA+GGG - chr8.4:59203248-59203267 None:intergenic 35.0%
! GCAAGTTCTTTTATCTTTGC+AGG + chr8.4:59204011-59204030 MS.gene60124:CDS 35.0%
!! TTGCAGCTTTGAAATGATCA+CGG - chr8.4:59203430-59203449 None:intergenic 35.0%
AAACATGCGCTAAAGCTATC+AGG - chr8.4:59203367-59203386 None:intergenic 40.0%
AAACGATTGCATATGGCTCA+CGG + chr8.4:59203494-59203513 MS.gene60124:intron 40.0%
AACTGCAGCCCTTCTAAATT+CGG + chr8.4:59203578-59203597 MS.gene60124:intron 40.0%
ACTATGATTCCTCTTGGTCT+TGG + chr8.4:59203195-59203214 MS.gene60124:intron 40.0%
CAAATATGTCATTGGTACCG+AGG - chr8.4:59203249-59203268 None:intergenic 40.0%
CCATCATCATTGAACTTTGC+AGG - chr8.4:59203955-59203974 None:intergenic 40.0%
CCTGCAAAGTTCAATGATGA+TGG + chr8.4:59203952-59203971 MS.gene60124:CDS 40.0%
CGCATGTTTAGTAATGCGTT+GGG + chr8.4:59203378-59203397 MS.gene60124:intron 40.0%
GAGTATAACTACTCTGTCAC+AGG + chr8.4:59203004-59203023 MS.gene60124:CDS 40.0%
GATGGCGATTTGATTGATGA+AGG + chr8.4:59203970-59203989 MS.gene60124:CDS 40.0%
GCTGCAAATTGTCAAAGTGA+AGG + chr8.4:59203443-59203462 MS.gene60124:intron 40.0%
GTCAAAGTGAAGGAACGTAA+CGG + chr8.4:59203453-59203472 MS.gene60124:intron 40.0%
GTCTAGACCACCAAATGTTT+GGG + chr8.4:59203789-59203808 MS.gene60124:CDS 40.0%
GTGACAGAGTAGTTATACTC+AGG - chr8.4:59203004-59203023 None:intergenic 40.0%
GTTGCAAAGGTAGGAATCAT+TGG - chr8.4:59203163-59203182 None:intergenic 40.0%
TCATCATTGAACTTTGCAGG+TGG - chr8.4:59203952-59203971 None:intergenic 40.0%
TGGCGATTTGATTGATGAAG+GGG + chr8.4:59203972-59203991 MS.gene60124:CDS 40.0%
TTTGATTGATGAAGGGGCTA+AGG + chr8.4:59203978-59203997 MS.gene60124:CDS 40.0%
! AGTTTTTGCCTTGAAGCCTT+AGG - chr8.4:59204044-59204063 None:intergenic 40.0%
! GTTTTTGCCTTGAAGCCTTA+GGG - chr8.4:59204043-59204062 None:intergenic 40.0%
!! GAAATGATCACGGTGTTGTT+GGG - chr8.4:59203420-59203439 None:intergenic 40.0%
!! GCAGCAATTGAGTTGAGTAA+AGG + chr8.4:59202830-59202849 MS.gene60124:intron 40.0%
!! GGATGACTCTAGTTTGTTCT+AGG + chr8.4:59203278-59203297 MS.gene60124:intron 40.0%
!! TGAAATGATCACGGTGTTGT+TGG - chr8.4:59203421-59203440 None:intergenic 40.0%
AAAGTGAAGGAACGTAACGG+TGG + chr8.4:59203456-59203475 MS.gene60124:intron 45.0%
ATCAAAGTGGCAGCTCTTTC+TGG + chr8.4:59202674-59202693 MS.gene60124:CDS 45.0%
ATGCGCTAAAGCTATCAGGT+AGG - chr8.4:59203363-59203382 None:intergenic 45.0%
CAAAAGAAGACGAACCAGCA+CGG - chr8.4:59202736-59202755 None:intergenic 45.0%
CACTATCATCTACGCCAAGT+TGG + chr8.4:59203868-59203887 MS.gene60124:CDS 45.0%
CGAAAAGCTTCAACAAGAGG+TGG - chr8.4:59202941-59202960 None:intergenic 45.0%
CGTCTAGACCACCAAATGTT+TGG + chr8.4:59203788-59203807 MS.gene60124:CDS 45.0%
CTTTGCAGGCATTTACCCTA+AGG + chr8.4:59204025-59204044 MS.gene60124:CDS 45.0%
GCATTTACCCTAAGGCTTCA+AGG + chr8.4:59204033-59204052 MS.gene60124:CDS 45.0%
GCGCATGTTTAGTAATGCGT+TGG + chr8.4:59203377-59203396 MS.gene60124:intron 45.0%
TGCGCTAAAGCTATCAGGTA+GGG - chr8.4:59203362-59203381 None:intergenic 45.0%
TGGCGAAAAGCTTCAACAAG+AGG - chr8.4:59202944-59202963 None:intergenic 45.0%
!! ATAAGCACTGGAGGAGACTT+TGG + chr8.4:59203832-59203851 MS.gene60124:CDS 45.0%
!! TGATTCCTCTTGGTCTTGGT+AGG + chr8.4:59203199-59203218 MS.gene60124:intron 45.0%
AAGACGAACCAGCACGGATA+AGG - chr8.4:59202730-59202749 None:intergenic 50.0%
ACCGAGGGATGTATCAACGT+GGG - chr8.4:59203233-59203252 None:intergenic 50.0%
AGACCACCAAATGTTTGGGC+TGG + chr8.4:59203793-59203812 MS.gene60124:CDS 50.0%
GCAGCTGCAATCATAAGCAC+TGG + chr8.4:59203820-59203839 MS.gene60124:CDS 50.0%
GCTTTGCCAGCCCAAACATT+TGG - chr8.4:59203802-59203821 None:intergenic 50.0%
TACCGAGGGATGTATCAACG+TGG - chr8.4:59203234-59203253 None:intergenic 50.0%
TTGCCAGCCCAAACATTTGG+TGG - chr8.4:59203799-59203818 None:intergenic 50.0%
!! GCTGCAATCATAAGCACTGG+AGG + chr8.4:59203823-59203842 MS.gene60124:CDS 50.0%
AGGGATGTATCAACGTGGGG+AGG - chr8.4:59203229-59203248 None:intergenic 55.0%
CAGCACGGATAAGGCCTGAA+TGG - chr8.4:59202721-59202740 None:intergenic 55.0%
CCCCACGTTGATACATCCCT+CGG + chr8.4:59203229-59203248 MS.gene60124:intron 55.0%
CCGAGGGATGTATCAACGTG+GGG - chr8.4:59203232-59203251 None:intergenic 55.0%
GGCAGCAGCAGTGATCAAAG+TGG + chr8.4:59202661-59202680 MS.gene60124:CDS 55.0%
GGGATGTATCAACGTGGGGA+GGG - chr8.4:59203228-59203247 None:intergenic 55.0%
GGTTGCCTACCAAGACCAAG+AGG - chr8.4:59203207-59203226 None:intergenic 55.0%
! AGCACTGGAGGAGACTTTGG+CGG + chr8.4:59203835-59203854 MS.gene60124:CDS 55.0%
! CATTCAGGCCTTATCCGTGC+TGG + chr8.4:59202719-59202738 MS.gene60124:CDS 55.0%
ACTGGAGGAGACTTTGGCGG+GGG + chr8.4:59203838-59203857 MS.gene60124:CDS 60.0%
CACTGGAGGAGACTTTGGCG+GGG + chr8.4:59203837-59203856 MS.gene60124:CDS 60.0%
GCACTGGAGGAGACTTTGGC+GGG + chr8.4:59203836-59203855 MS.gene60124:CDS 60.0%


Chromosome Type Strat End Strand Name
chr8.4 gene 59202659 59204065 59202659 ID=MS.gene60124
chr8.4 mRNA 59202659 59204065 59202659 ID=MS.gene60124.t1;Parent=MS.gene60124
chr8.4 exon 59202659 59202740 59202659 ID=MS.gene60124.t1.exon1;Parent=MS.gene60124.t1
chr8.4 CDS 59202659 59202740 59202659 ID=cds.MS.gene60124.t1;Parent=MS.gene60124.t1
chr8.4 exon 59202834 59203025 59202834 ID=MS.gene60124.t1.exon2;Parent=MS.gene60124.t1
chr8.4 CDS 59202834 59203025 59202834 ID=cds.MS.gene60124.t1;Parent=MS.gene60124.t1
chr8.4 exon 59203761 59204065 59203761 ID=MS.gene60124.t1.exon3;Parent=MS.gene60124.t1
chr8.4 CDS 59203761 59204065 59203761 ID=cds.MS.gene60124.t1;Parent=MS.gene60124.t1
Gene Sequence

>MS.gene60124

ATGGCAGCAGCAGTGATCAAAGTGGCAGCTCTTTCTGGCTCCCTCAGAAAAACTTCATACCATTCAGGCCTTATCCGTGCTGGTTCGTCTTCTTTTGTTTTGTTCAAGTGTTTCTTCTTTGATATGTTTAGAAATTGTTGTAGTATTGAATTTGCATGGTTGATGATGATTGCAGCAATTGAGTTGAGTAAAGGAGCAATTGAAGGCATTGAGATTGAGTTCATTGATATTTCAACTCTACCAATGTACAACACTGATCTTGAAAACAAGGGAACTTATCCACCTCTTGTTGAAGCTTTTCGCCACAAGATTCTTCAAGCTGATAGTGTTCTTTTTGCTTCTCCTGAGTATAACTACTCTGTCACAGGTACATTTTCTTGTACTTCTGTAATGATTTAGATTGTTTTTTTGATATAACAGCTCCTTGTAATAGAAGAAGAAAAGAAACGAAAAATCGCAAGATTATGAGACACGATTTTTGTTCAAGAAGTTCGGCTTCAGCCAATGATTCCTACCTTTGCAACTATTTATATAATACTATGATTCCTCTTGGTCTTGGTAGGCAACCCTCCCCACGTTGATACATCCCTCGGTACCAATGACATATTTGATTGCAAATGGATGACTCTAGTTTGTTCTAGGCCATAATTGCAATATTTTGGGTTGAATCTCTGTTGACACTATCAACATTTAAAGGGTGCCCTACCTGATAGCTTTAGCGCATGTTTAGTAATGCGTTGGGAACATTTTTCTCGTGTCCCAACAACACCGTGATCATTTCAAAGCTGCAAATTGTCAAAGTGAAGGAACGTAACGGTGGACAATCCCATTGCAAAAACGATTGCATATGGCTCACGGATAATATCATAATATGAACTGTAGTATTTAAATACAAGTTATACACTGTAAACTTAAAATGAACTGCAGCCCTTCTAAATTCGGAGATATAGTCACAAATGATCGAACTTCCTTATCAAACACAGACTTAGTGTGCCATTTCTACCTATCAATTTTTTAATTACAAAAAATGAATTAAGACTGTGAATGAATCATGAAATATATATAAAGCTTATTTCTATCTTCACCTTTGTTTTGTGTTTAGCTCCATTGAAGAATGCAATTGATTGGGCGTCTAGACCACCAAATGTTTGGGCTGGCAAAGCAGCTGCAATCATAAGCACTGGAGGAGACTTTGGCGGGGGAAGATCACACTATCATCTACGCCAAGTTGGAATTTATATTGATCTTCATTTCATCAATAAACCTGAGTTCTTTCTAAGAGCATTTGAGCCACCTGCAAAGTTCAATGATGATGGCGATTTGATTGATGAAGGGGCTAAGGATAAGCTTAAGCAAGTTCTTTTATCTTTGCAGGCATTTACCCTAAGGCTTCAAGGCAAAAACTGA

Protein sequence

>MS.gene60124.t1

MAAAVIKVAALSGSLRKTSYHSGLIRAAIELSKGAIEGIEIEFIDISTLPMYNTDLENKGTYPPLVEAFRHKILQADSVLFASPEYNYSVTAPLKNAIDWASRPPNVWAGKAAAIISTGGDFGGGRSHYHLRQVGIYIDLHFINKPEFFLRAFEPPAKFNDDGDLIDEGAKDKLKQVLLSLQAFTLRLQGKN