AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene60374


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene60374.t1 MTR_4g098850 94.521 73 4 0 1 73 281 353 1.44e-42 146
MS.gene60374.t1 MTR_5g014090 53.398 103 16 2 2 73 5 106 6.97e-23 90.1
MS.gene60374.t1 MTR_5g014090 53.398 103 16 2 2 73 5 106 8.97e-23 90.1
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene60374.t1 AT4G18010 46.296 108 21 2 2 73 5 111 1.35e-19 80.9
MS.gene60374.t1 AT4G18010 46.296 108 21 2 2 73 5 111 1.36e-19 80.9

Find 10 sgRNAs with CRISPR-Local

Find 126 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTTCATTAATGCAGGTGTTT+TGG 0.294116 8.1:+23796112 MS.gene60374:intron
ATGGTTGAATATAAAGCAAA+AGG 0.473863 8.1:+23796155 MS.gene60374:CDS
AGCATCATACCTCACTTCCT+TGG 0.478675 8.1:-23797933 None:intergenic
CATCCACAGTGATGAAGAAA+TGG 0.502176 8.1:+23796136 MS.gene60374:CDS
ACATAAATACCAAGGAAGTG+AGG 0.640425 8.1:+23797924 MS.gene60374:CDS
ACTGATGAGAGTGAAGATGA+TGG 0.645910 8.1:+23796213 MS.gene60374:CDS
AGCACAATGAAAACAATGAG+GGG 0.654951 8.1:+23795996 None:intergenic
TGCTCAATACATAAATACCA+AGG 0.661086 8.1:+23797916 MS.gene60374:CDS
CAACCATTTCTTCATCACTG+TGG 0.674705 8.1:-23796139 None:intergenic
AATGAGGGGAAAACGCTCAG+AGG 0.681871 8.1:+23796010 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAATTATATGAACAACTAAA+AGG - chr8.1:23796278-23796297 None:intergenic 15.0%
!! AAATTGTTAGTATTATATTG+AGG + chr8.1:23796450-23796469 MS.gene60374:intron 15.0%
!! AATTGTTAGTATTATATTGA+GGG + chr8.1:23796451-23796470 MS.gene60374:intron 15.0%
!! ATTAATGAAACCAAATTATA+AGG - chr8.1:23796102-23796121 None:intergenic 15.0%
!! ATTAATTTCATATACTTTCT+CGG - chr8.1:23797745-23797764 None:intergenic 15.0%
!! CTTATTTATTCATCTTAAAA+AGG + chr8.1:23796776-23796795 MS.gene60374:intron 15.0%
!!! CTTTTTAAGATGAATAAATA+AGG - chr8.1:23796778-23796797 None:intergenic 15.0%
!!! TATTTTGGATGTTTTTTAAT+TGG + chr8.1:23797189-23797208 MS.gene60374:intron 15.0%
!! AATTTCTATTCTATTCTATG+AGG + chr8.1:23796054-23796073 MS.gene60374:intron 20.0%
!! ATTACATAAATATGTGTATG+CGG - chr8.1:23797047-23797066 None:intergenic 20.0%
!! TCAAATTATCCTTATTAGAA+TGG + chr8.1:23797557-23797576 MS.gene60374:intron 20.0%
!! TTTGTTGAAACCTTATAATT+TGG + chr8.1:23796089-23796108 MS.gene60374:intron 20.0%
!!! ATTAAGCAGTTTTTAGATTT+AGG - chr8.1:23797131-23797150 None:intergenic 20.0%
!!! GGGTATTTTTTTATAAACAT+GGG + chr8.1:23797381-23797400 MS.gene60374:intron 20.0%
!!! TCTTTTCATAGTTAAAATGT+GGG + chr8.1:23797302-23797321 MS.gene60374:intron 20.0%
!!! TGGGTATTTTTTTATAAACA+TGG + chr8.1:23797380-23797399 MS.gene60374:intron 20.0%
!!! TTTTTTATAAACATGGGTAT+GGG + chr8.1:23797387-23797406 MS.gene60374:intron 20.0%
!!! TTTTTTTATAAACATGGGTA+TGG + chr8.1:23797386-23797405 MS.gene60374:intron 20.0%
! AATTACCCACAAATTTAAGT+GGG + chr8.1:23796979-23796998 MS.gene60374:intron 25.0%
! AATTATCCTTATTAGAATGG+TGG + chr8.1:23797560-23797579 MS.gene60374:intron 25.0%
! ATAGTTAAAATGTGGGTAAT+GGG + chr8.1:23797309-23797328 MS.gene60374:intron 25.0%
! ATATGAACAACTAAAAGGAA+AGG - chr8.1:23796273-23796292 None:intergenic 25.0%
! ATGATCAAATAGACATTCTA+AGG - chr8.1:23797456-23797475 None:intergenic 25.0%
! ATGGTTGAATATAAAGCAAA+AGG + chr8.1:23796155-23796174 MS.gene60374:CDS 25.0%
! ATTGATGAGATATGAATAGA+AGG + chr8.1:23796329-23796348 MS.gene60374:intron 25.0%
! TAATTACCCACAAATTTAAG+TGG + chr8.1:23796978-23796997 MS.gene60374:intron 25.0%
! TCATAAAATAGTAAGAATCG+TGG - chr8.1:23797222-23797241 None:intergenic 25.0%
! TTACATAAATATGTGTATGC+GGG - chr8.1:23797046-23797065 None:intergenic 25.0%
!! GTCTTTTCATAGTTAAAATG+TGG + chr8.1:23797301-23797320 MS.gene60374:intron 25.0%
!! TAATTTGGTTTCATTAATGC+AGG + chr8.1:23796104-23796123 MS.gene60374:intron 25.0%
!!! ACAGTTTTTTAGGGATATTT+TGG + chr8.1:23797174-23797193 MS.gene60374:intron 25.0%
!!! ATTGAAAGTGACTTGTTTTT+TGG - chr8.1:23796831-23796850 None:intergenic 25.0%
AAAAAATATCCTCATTGGGT+AGG + chr8.1:23796928-23796947 MS.gene60374:intron 30.0%
AAAAAGCCACCATTCTAATA+AGG - chr8.1:23797569-23797588 None:intergenic 30.0%
AAAAATATCCTCATTGGGTA+GGG + chr8.1:23796929-23796948 MS.gene60374:intron 30.0%
AGACAATGCATAATATATGC+GGG + chr8.1:23796728-23796747 MS.gene60374:intron 30.0%
AGATGAATAAATAAGGTGTG+TGG - chr8.1:23796771-23796790 None:intergenic 30.0%
ATATGAATAGAAGGAAAGAG+AGG + chr8.1:23796338-23796357 MS.gene60374:intron 30.0%
ATGAATAAATAAGGTGTGTG+GGG - chr8.1:23796769-23796788 None:intergenic 30.0%
CATAGTTAAAATGTGGGTAA+TGG + chr8.1:23797308-23797327 MS.gene60374:intron 30.0%
CATGACATGAAAGCTTATAA+AGG - chr8.1:23796536-23796555 None:intergenic 30.0%
CCGTAAAAAATATCCTCATT+GGG + chr8.1:23796924-23796943 MS.gene60374:intron 30.0%
GAGACAATGCATAATATATG+CGG + chr8.1:23796727-23796746 MS.gene60374:intron 30.0%
GATGAATAAATAAGGTGTGT+GGG - chr8.1:23796770-23796789 None:intergenic 30.0%
TAAATTTGTGGGTAATTACC+CGG - chr8.1:23796976-23796995 None:intergenic 30.0%
TAGTCTTCCAAAATTGACAT+GGG + chr8.1:23797660-23797679 MS.gene60374:intron 30.0%
TGCTCAATACATAAATACCA+AGG + chr8.1:23797916-23797935 MS.gene60374:CDS 30.0%
! AACATCTCATTTCTTTTCCA+AGG - chr8.1:23797609-23797628 None:intergenic 30.0%
! AAGAGGGAGTATAAAATAGT+TGG - chr8.1:23796642-23796661 None:intergenic 30.0%
! AGAGGGAGTATAAAATAGTT+GGG - chr8.1:23796641-23796660 None:intergenic 30.0%
! TCAAATAGACATTCTAAGGA+TGG - chr8.1:23797452-23797471 None:intergenic 30.0%
! TTAGTCTTCCAAAATTGACA+TGG + chr8.1:23797659-23797678 MS.gene60374:intron 30.0%
! TTTCATTAATGCAGGTGTTT+TGG + chr8.1:23796112-23796131 MS.gene60374:intron 30.0%
!!! ACAATTGCTTTTTTCTGTTC+TGG - chr8.1:23796411-23796430 None:intergenic 30.0%
!!! CCAATGAGGATATTTTTTAC+GGG - chr8.1:23796926-23796945 None:intergenic 30.0%
!!! CCCAATGAGGATATTTTTTA+CGG - chr8.1:23796927-23796946 None:intergenic 30.0%
AATTTACAAGTGTGACGAAG+AGG - chr8.1:23796659-23796678 None:intergenic 35.0%
ACAACTAGAGAGCAATATGA+TGG - chr8.1:23796302-23796321 None:intergenic 35.0%
ATCCCTAAAAAACTGTACAC+AGG - chr8.1:23797170-23797189 None:intergenic 35.0%
ATGAAGCTGAAATTGTTCCT+TGG + chr8.1:23797589-23797608 MS.gene60374:intron 35.0%
ATTTACAAGTGTGACGAAGA+GGG - chr8.1:23796658-23796677 None:intergenic 35.0%
CCATACCCACTTAAATTTGT+GGG - chr8.1:23796987-23797006 None:intergenic 35.0%
CCCGTAAAAAATATCCTCAT+TGG + chr8.1:23796923-23796942 MS.gene60374:intron 35.0%
CTAAAAAACTGTACACAGGA+GGG - chr8.1:23797166-23797185 None:intergenic 35.0%
GACAATGCATAATATATGCG+GGG + chr8.1:23796729-23796748 MS.gene60374:intron 35.0%
GAGTATGACACTAAAGTTGT+TGG + chr8.1:23797335-23797354 MS.gene60374:intron 35.0%
GATTAACTGTTATCGTGATG+AGG + chr8.1:23797788-23797807 MS.gene60374:intron 35.0%
TATAAAATAGTTGGGAGGAG+TGG - chr8.1:23796633-23796652 None:intergenic 35.0%
TCATATTGCTCTCTAGTTGT+TGG + chr8.1:23796302-23796321 MS.gene60374:intron 35.0%
TCCATACCCACTTAAATTTG+TGG - chr8.1:23796988-23797007 None:intergenic 35.0%
TTATAAACATGGGTATGGGA+AGG + chr8.1:23797391-23797410 MS.gene60374:intron 35.0%
! CTTAAAACGAGTGTCGTATA+GGG + chr8.1:23796871-23796890 MS.gene60374:intron 35.0%
! TCTTAAAACGAGTGTCGTAT+AGG + chr8.1:23796870-23796889 MS.gene60374:intron 35.0%
! TTTGGAGTCTAGTAAACTAG+TGG - chr8.1:23796813-23796832 None:intergenic 35.0%
!! TTCAAAGCATTGACATAGTC+TGG - chr8.1:23796602-23796621 None:intergenic 35.0%
!!! ATACGACACTCGTTTTAAGA+CGG - chr8.1:23796870-23796889 None:intergenic 35.0%
!!! CTCCTGTGTACAGTTTTTTA+GGG + chr8.1:23797165-23797184 MS.gene60374:intron 35.0%
!!! TATTTTTTACGGGTGTCCAT+CGG - chr8.1:23796916-23796935 None:intergenic 35.0%
ACTGATGAGAGTGAAGATGA+TGG + chr8.1:23796213-23796232 MS.gene60374:CDS 40.0%
ATAATATATGCGGGGAGTCA+GGG + chr8.1:23796737-23796756 MS.gene60374:intron 40.0%
CAACCATTTCTTCATCACTG+TGG - chr8.1:23796142-23796161 None:intergenic 40.0%
CACTATAGTACCTTACCGAT+AGG + chr8.1:23797416-23797435 MS.gene60374:intron 40.0%
CATCCACAGTGATGAAGAAA+TGG + chr8.1:23796136-23796155 MS.gene60374:CDS 40.0%
CCCACAAATTTAAGTGGGTA+TGG + chr8.1:23796984-23797003 MS.gene60374:intron 40.0%
CCTAAAAAACTGTACACAGG+AGG - chr8.1:23797167-23797186 None:intergenic 40.0%
CCTTCTCTGTAAACTAAACC+AGG - chr8.1:23796490-23796509 None:intergenic 40.0%
GTCGTATAGGGATGGTAATT+GGG + chr8.1:23796883-23796902 MS.gene60374:intron 40.0%
TGTCGTATAGGGATGGTAAT+TGG + chr8.1:23796882-23796901 MS.gene60374:intron 40.0%
! AAATATGTGTATGCGGGTTG+CGG - chr8.1:23797040-23797059 None:intergenic 40.0%
! AGACATTCTAAGGATGGCAA+TGG - chr8.1:23797446-23797465 None:intergenic 40.0%
! CCTGGTTTAGTTTACAGAGA+AGG + chr8.1:23796487-23796506 MS.gene60374:intron 40.0%
! CGTTTCACCCATGTCAATTT+TGG - chr8.1:23797670-23797689 None:intergenic 40.0%
! GACATTCTAAGGATGGCAAT+GGG - chr8.1:23797445-23797464 None:intergenic 40.0%
! GGGAGTATAAAATAGTTGGG+AGG - chr8.1:23796638-23796657 None:intergenic 40.0%
!! TGGTTTGGTGGTGTTAGTTT+AGG - chr8.1:23797709-23797728 None:intergenic 40.0%
!!! CCTCCTGTGTACAGTTTTTT+AGG + chr8.1:23797164-23797183 MS.gene60374:intron 40.0%
AAACCCTGCTTTCATGGATC+CGG + chr8.1:23796954-23796973 MS.gene60374:intron 45.0%
AAACTGTACACAGGAGGGAA+CGG - chr8.1:23797161-23797180 None:intergenic 45.0%
AGGGTAAAACCCTGCTTTCA+TGG + chr8.1:23796948-23796967 MS.gene60374:intron 45.0%
CAATGGGTATCCTATCGGTA+AGG - chr8.1:23797429-23797448 None:intergenic 45.0%
CATAATATATGCGGGGAGTC+AGG + chr8.1:23796736-23796755 MS.gene60374:intron 45.0%
GAGGGTGATTCTAATTGTCC+TGG + chr8.1:23796469-23796488 MS.gene60374:intron 45.0%
GATGGCAATGGGTATCCTAT+CGG - chr8.1:23797434-23797453 None:intergenic 45.0%
! AAACGAGTGTCGTATAGGGA+TGG + chr8.1:23796875-23796894 MS.gene60374:intron 45.0%
AACCCTGCTTTCATGGATCC+GGG + chr8.1:23796955-23796974 MS.gene60374:intron 50.0%
CACTAAAGTTGTTGGCCACG+AGG + chr8.1:23797343-23797362 MS.gene60374:intron 50.0%
GTGAAACGAAACACGCTGCA+AGG + chr8.1:23797682-23797701 MS.gene60374:intron 50.0%
TACCCGGATCCATGAAAGCA+GGG - chr8.1:23796960-23796979 None:intergenic 50.0%
TTACCCGGATCCATGAAAGC+AGG - chr8.1:23796961-23796980 None:intergenic 50.0%
! GGGTTTTACCCTACCCAATG+AGG - chr8.1:23796940-23796959 None:intergenic 50.0%
! GGTTAGTCGCACGTTTGGTT+TGG - chr8.1:23797724-23797743 None:intergenic 50.0%
! TTCTCGGTTAGTCGCACGTT+TGG - chr8.1:23797729-23797748 None:intergenic 50.0%
!! TAGTCGCACGTTTGGTTTGG+TGG - chr8.1:23797721-23797740 None:intergenic 50.0%
!!! CGACACTCGTTTTAAGACGG+AGG - chr8.1:23796867-23796886 None:intergenic 50.0%
ACGAGGATACGCGTTGGGTA+TGG + chr8.1:23797360-23797379 MS.gene60374:intron 55.0%
AGTGGGTATGGACGCGAGTA+AGG + chr8.1:23796996-23797015 MS.gene60374:intron 55.0%
ATACCCAACGCGTATCCTCG+TGG - chr8.1:23797361-23797380 None:intergenic 55.0%
CGAGGATACGCGTTGGGTAT+GGG + chr8.1:23797361-23797380 MS.gene60374:intron 55.0%
GTAATTGGGCCCAAGTCCGA+TGG + chr8.1:23796897-23796916 MS.gene60374:intron 55.0%
GTGGGTATGGACGCGAGTAA+GGG + chr8.1:23796997-23797016 MS.gene60374:intron 55.0%
TACGGGTGTCCATCGGACTT+GGG - chr8.1:23796909-23796928 None:intergenic 55.0%
TTACGGGTGTCCATCGGACT+TGG - chr8.1:23796910-23796929 None:intergenic 55.0%
TGGCCACGAGGATACGCGTT+GGG + chr8.1:23797355-23797374 MS.gene60374:intron 60.0%
TTGGCCACGAGGATACGCGT+TGG + chr8.1:23797354-23797373 MS.gene60374:intron 60.0%
! TGTATGCGGGTTGCGGAGCT+GGG - chr8.1:23797033-23797052 None:intergenic 60.0%
! GTGTATGCGGGTTGCGGAGC+TGG - chr8.1:23797034-23797053 None:intergenic 65.0%


Chromosome Type Strat End Strand Name
chr8.1 gene 23796011 23797945 23796011 ID=MS.gene60374
chr8.1 mRNA 23796011 23797945 23796011 ID=MS.gene60374.t1;Parent=MS.gene60374
chr8.1 exon 23796011 23796031 23796011 ID=MS.gene60374.t1.exon1;Parent=MS.gene60374.t1
chr8.1 CDS 23796011 23796031 23796011 ID=cds.MS.gene60374.t1;Parent=MS.gene60374.t1
chr8.1 exon 23796126 23796234 23796126 ID=MS.gene60374.t1.exon2;Parent=MS.gene60374.t1
chr8.1 CDS 23796126 23796234 23796126 ID=cds.MS.gene60374.t1;Parent=MS.gene60374.t1
chr8.1 exon 23797855 23797945 23797855 ID=MS.gene60374.t1.exon3;Parent=MS.gene60374.t1
chr8.1 CDS 23797855 23797945 23797855 ID=cds.MS.gene60374.t1;Parent=MS.gene60374.t1
Gene Sequence

>MS.gene60374

ATGAGGGGAAAACGCTCAGAGGTAAGCTTTAACATGCTTTGTAAATTTCTATTCTATTCTATGAGGATACATATATAATTTGTTGAAACCTTATAATTTGGTTTCATTAATGCAGGTGTTTTGGTCATCCACAGTGATGAAGAAATGGTTGAATATAAAGCAAAAGGTGTATGATTTTAGTGAAGATGAAGCTAACACTGAAACTGATGAGAGTGAAGATGATGGTGTGTGCTTCATTATGCACTATATATTCTTTTTTCCTTTCCTTTTAGTTGTTCATATAATTTGCCATCATATTGCTCTCTAGTTGTTGGTTTAATTGATGAGATATGAATAGAAGGAAAGAGAGGATATAGATATTTTAATTAAATATTCTCTTTTGATTAATATGAGTGAACCAGAACAGAAAAAAGCAATTGTTTACTAGTCTATTTTCTTTAAATTGTTAGTATTATATTGAGGGTGATTCTAATTGTCCTGGTTTAGTTTACAGAGAAGGATTTTCTAAATTAACTTTTTATACCTTTATAAGCTTTCATGTCATGCATTATGAGTATAGATTCATAAATTTGTTTGCTATATTGTGTTCCAGACTATGTCAATGCTTTGAATTAGTTTTCCACTCCTCCCAACTATTTTATACTCCCTCTTCGTCACACTTGTAAATTAAATATTTCTAAAAATAAACGTCACTTTGTACATTTGAGTTTAAGACAGAGACAATGCATAATATATGCGGGGAGTCAGGGTTTGAACCCCACACACCTTATTTATTCATCTTAAAAAGGTGAATTTCTAGCCACTAGTTTACTAGACTCCAAAAAACAAGTCACTTTCAATTTTCAATGAAATTCCTCCGTCTTAAAACGAGTGTCGTATAGGGATGGTAATTGGGCCCAAGTCCGATGGACACCCGTAAAAAATATCCTCATTGGGTAGGGTAAAACCCTGCTTTCATGGATCCGGGTAATTACCCACAAATTTAAGTGGGTATGGACGCGAGTAAGGGAATTATAATGCCCAGCTCCGCAACCCGCATACACATATTTATGTAATATGATAAATACTTTATTTATTTTTTGTGAACAATTAATTAATTTAGCTATTTAAACATAAACCTAAATCTAAAAACTGCTTAATACAGTAACCGTTCCCTCCTGTGTACAGTTTTTTAGGGATATTTTGGATGTTTTTTAATTGGTTGAATACCACGATTCTTACTATTTTATGAGTTGATTTAAAAAAAAAAAATATGATCATAGTTTTATTTCAATTGTGATGTTTTTTATTGTCTTTTCATAGTTAAAATGTGGGTAATGGGTGCGAGTATGACACTAAAGTTGTTGGCCACGAGGATACGCGTTGGGTATGGGTATTTTTTTATAAACATGGGTATGGGAAGGTGCACTATAGTACCTTACCGATAGGATACCCATTGCCATCCTTAGAATGTCTATTTGATCATTTCACACTTATTATTTTTTTTTTTTAACAATCATTTCATACGTATTATGAAAAGATGTTAAATGAGAGAGATAAGACTTTATCAAATTATCCTTATTAGAATGGTGGCTTTTTATGAAGCTGAAATTGTTCCTTGGAAAAGAAATGAGATGTTTTGAAGTTATGTTTTTTTTAATTAGTTAAATTAGTCTTCCAAAATTGACATGGGTGAAACGAAACACGCTGCAAGGTCCTAAACTAACACCACCAAACCAAACGTGCGACTAACCGAGAAAGTATATGAAATTAATATAACTGTTCTGTAAAATTAAATGATTAACTGTTATCGTGATGAGGTCATAGTTAAAATCGTTATATGTGTTTCAATATTTGTAATTCAGATACATCTTGTAAAGACTACGAGATGAAACAACACAGAAGAAGAAAATCAGAAACTTTGCGTGCTCAATACATAAATACCAAGGAAGTGAG

Protein sequence

>MS.gene60374.t1

MRGKRSEVFWSSTVMKKWLNIKQKVYDFSEDEANTETDESEDDDTSCKDYEMKQHRRRKSETLRAQYINTKEV