AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene61579


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene61579.t1 MTR_4g049340 99.163 239 2 0 1 239 1 239 8.43e-172 473
MS.gene61579.t1 MTR_7g085070 52.245 245 110 3 1 239 3 246 1.86e-79 239
MS.gene61579.t1 MTR_6g033010 51.489 235 108 2 4 233 5 238 9.89e-74 224
MS.gene61579.t1 MTR_6g033010 51.020 196 90 2 4 194 5 199 6.11e-53 170
MS.gene61579.t1 MTR_6g033010 51.309 191 87 2 4 189 5 194 5.39e-52 168
MS.gene61579.t1 MTR_7g088310 34.694 196 110 6 49 239 50 232 1.85e-25 100
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene61579.t1 AT3G04090 46.522 230 119 2 1 230 2 227 5.30e-61 192
MS.gene61579.t1 AT5G18290 43.033 244 133 3 1 239 1 243 2.48e-55 177
MS.gene61579.t1 AT5G18290 43.033 244 133 3 1 239 1 243 2.48e-55 177
MS.gene61579.t1 AT3G56950 29.461 241 156 5 1 238 1 230 9.76e-25 98.6
MS.gene61579.t1 AT3G56950 29.461 241 156 5 1 238 1 230 1.44e-24 98.6

Find 58 sgRNAs with CRISPR-Local

Find 66 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ATCCTCACAATAAGCCTTAT+TGG 0.201441 4.1:+48378119 MS.gene61579:CDS
TATTCTTCTTGTTATGCTTA+AGG 0.242398 4.1:+48378400 MS.gene61579:CDS
TTCTGTGGCAACTTTGGTTT+TGG 0.253700 4.1:+48378457 MS.gene61579:CDS
ATTCTTCTTGTTATGCTTAA+GGG 0.255110 4.1:+48378401 MS.gene61579:CDS
CACCTAAGATTCTTCCAATA+AGG 0.292588 4.1:-48378133 None:intergenic
ATTATTGGTTCTGGTTATAC+TGG 0.295871 4.1:+48378482 MS.gene61579:CDS
CCCTGCTAATGCATTTGGTT+GGG 0.316346 4.1:+48378517 MS.gene61579:CDS
TCTATTTATGTCACCGGTTA+AGG 0.319094 4.1:+48378637 MS.gene61579:CDS
ACAATTTCCTTCTACACATT+AGG 0.335641 4.1:+48378179 MS.gene61579:CDS
ATGAACCCTGCTAATGCATT+TGG 0.350303 4.1:+48378512 MS.gene61579:CDS
AGCCTTATTGGAAGAATCTT+AGG 0.350741 4.1:+48378131 MS.gene61579:CDS
CAAGCTTTCTTCTCCTTAAC+CGG 0.351841 4.1:-48378650 None:intergenic
TGAGGATAATGATTGTGTTT+AGG 0.376446 4.1:-48378103 None:intergenic
TTTGCTTTCTGTGGCAACTT+TGG 0.391117 4.1:+48378451 MS.gene61579:CDS
TTGAAGGTTGATTTGCATAC+AGG 0.396215 4.1:+48378335 MS.gene61579:CDS
TGCTTGAGCAGGAAACCTTA+TGG 0.401263 4.1:-48378234 None:intergenic
GGTGAAGGGTCCTTCTTTGA+AGG 0.406147 4.1:+48378319 MS.gene61579:CDS
TCTTAGTCCTAATGTGTAGA+AGG 0.412697 4.1:-48378186 None:intergenic
CATACAGGTGCTATTGTTGA+AGG 0.413505 4.1:+48378350 MS.gene61579:CDS
ACCATGTCTTTGTATTCTGT+TGG 0.422876 4.1:-48378299 None:intergenic
AATAGGCCAGGTAGTGAAAC+TGG 0.426892 4.1:-48378068 None:intergenic
AGGTTTCCTGCTCAAGCAAT+AGG 0.431036 4.1:+48378239 MS.gene61579:CDS
TGGTGCTAGTTCTGCTGCTA+TGG 0.435394 4.1:+48378604 MS.gene61579:CDS
CAAGCAATAGGTGGAGCAAT+TGG 0.444422 4.1:+48378251 MS.gene61579:CDS
GCCCAACCAAATGCATTAGC+AGG 0.448752 4.1:-48378518 None:intergenic
TGAACAATAAGCACAATACT+TGG 0.454730 4.1:+48378546 MS.gene61579:CDS
TTAGGATGGTTGTGATGAAT+AGG 0.455656 4.1:-48378085 None:intergenic
GCTCCACCTATTGCTTGAGC+AGG 0.471313 4.1:-48378245 None:intergenic
AAAGATAGAAGAGATGAATC+TGG 0.478574 4.1:-48378209 None:intergenic
TCGATTTCTATTTATGTCAC+CGG 0.478707 4.1:+48378631 MS.gene61579:CDS
ATTCTGTTGGTATAACTTGA+AGG 0.478894 4.1:-48378286 None:intergenic
GCATTAGCAGGGTTCATAGA+AGG 0.483858 4.1:-48378506 None:intergenic
TGTTAAAATTGCATCTCCTA+TGG 0.493006 4.1:-48377973 None:intergenic
ACCCTGCTAATGCATTTGGT+TGG 0.505171 4.1:+48378516 MS.gene61579:CDS
TGTTGGTATAACTTGAAGGA+TGG 0.506984 4.1:-48378282 None:intergenic
TGCAAATCAACCTTCAAAGA+AGG 0.515327 4.1:-48378329 None:intergenic
CTTCAACCAGTTTCACTACC+TGG 0.524632 4.1:+48378062 MS.gene61579:CDS
GTGAAACTGGTTGAAGGTTG+AGG 0.525730 4.1:-48378055 None:intergenic
TAGAAGGAAATTGTGGATGA+AGG 0.526466 4.1:-48378170 None:intergenic
GAAGGTTTATTTGCTTTCTG+TGG 0.527749 4.1:+48378442 MS.gene61579:CDS
TACAGGTGCTATTGTTGAAG+GGG 0.543535 4.1:+48378352 MS.gene61579:CDS
AGCAAATAAACCTTCAACCA+AGG 0.552634 4.1:-48378434 None:intergenic
GATAATGATTGTGTTTAGGA+TGG 0.553340 4.1:-48378099 None:intergenic
CAGGTAGTGAAACTGGTTGA+AGG 0.557780 4.1:-48378061 None:intergenic
ATACAGGTGCTATTGTTGAA+GGG 0.562222 4.1:+48378351 MS.gene61579:CDS
ATGGTTGTGATGAATAGGCC+AGG 0.562488 4.1:-48378080 None:intergenic
TTCCAATAAGGCTTATTGTG+AGG 0.575912 4.1:-48378121 None:intergenic
CCCAACCAAATGCATTAGCA+GGG 0.593803 4.1:-48378517 None:intergenic
GCAGAACTAGCACCAATCAA+AGG 0.594305 4.1:-48378596 None:intergenic
GAACAATAAGCACAATACTT+GGG 0.597103 4.1:+48378547 MS.gene61579:CDS
TAATGTGTAGAAGGAAATTG+TGG 0.598806 4.1:-48378177 None:intergenic
AGAAGGAAATTGTGGATGAA+GGG 0.612204 4.1:-48378169 None:intergenic
GGAGTGATTAAATCAGCCAT+AGG 0.613343 4.1:+48377957 MS.gene61579:CDS
AGAATACAAAGACATGGTGA+AGG 0.621122 4.1:+48378304 MS.gene61579:CDS
TTTCCTGCTCAAGCAATAGG+TGG 0.642045 4.1:+48378242 MS.gene61579:CDS
GAATACAAAGACATGGTGAA+GGG 0.648042 4.1:+48378305 MS.gene61579:CDS
CTTATTGGAAGAATCTTAGG+TGG 0.659314 4.1:+48378134 MS.gene61579:CDS
ACCAACAGAATACAAAGACA+TGG 0.676539 4.1:+48378298 MS.gene61579:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATTCTTCTTGTTATGCTTAA+GGG + chr4.1:48378401-48378420 MS.gene61579:CDS 25.0%
!! TATTCTTCTTGTTATGCTTA+AGG + chr4.1:48378400-48378419 MS.gene61579:CDS 25.0%
!!! ACTTTGGTTTTGGTTATTAT+TGG + chr4.1:48378467-48378486 MS.gene61579:CDS 25.0%
AAAGATAGAAGAGATGAATC+TGG - chr4.1:48378212-48378231 None:intergenic 30.0%
ACAATTTCCTTCTACACATT+AGG + chr4.1:48378179-48378198 MS.gene61579:CDS 30.0%
ATTCTGTTGGTATAACTTGA+AGG - chr4.1:48378289-48378308 None:intergenic 30.0%
GAACAATAAGCACAATACTT+GGG + chr4.1:48378547-48378566 MS.gene61579:CDS 30.0%
GATAATGATTGTGTTTAGGA+TGG - chr4.1:48378102-48378121 None:intergenic 30.0%
TAATGTGTAGAAGGAAATTG+TGG - chr4.1:48378180-48378199 None:intergenic 30.0%
TCGATTTCTATTTATGTCAC+CGG + chr4.1:48378631-48378650 MS.gene61579:CDS 30.0%
TGAACAATAAGCACAATACT+TGG + chr4.1:48378546-48378565 MS.gene61579:CDS 30.0%
TGAGGATAATGATTGTGTTT+AGG - chr4.1:48378106-48378125 None:intergenic 30.0%
TGTTAAAATTGCATCTCCTA+TGG - chr4.1:48377976-48377995 None:intergenic 30.0%
! GGGAGCAATTTTATGTTTTT+TGG + chr4.1:48378567-48378586 MS.gene61579:CDS 30.0%
! TTTTGGATATGTCCTTTGAT+TGG + chr4.1:48378584-48378603 MS.gene61579:CDS 30.0%
!! ATTATTGGTTCTGGTTATAC+TGG + chr4.1:48378482-48378501 MS.gene61579:CDS 30.0%
!!! GTTTTGGTTATTATTGGTTC+TGG + chr4.1:48378473-48378492 MS.gene61579:CDS 30.0%
ACCAACAGAATACAAAGACA+TGG + chr4.1:48378298-48378317 MS.gene61579:CDS 35.0%
ACCATGTCTTTGTATTCTGT+TGG - chr4.1:48378302-48378321 None:intergenic 35.0%
AGAAGGAAATTGTGGATGAA+GGG - chr4.1:48378172-48378191 None:intergenic 35.0%
AGAATACAAAGACATGGTGA+AGG + chr4.1:48378304-48378323 MS.gene61579:CDS 35.0%
AGCAAATAAACCTTCAACCA+AGG - chr4.1:48378437-48378456 None:intergenic 35.0%
CACCTAAGATTCTTCCAATA+AGG - chr4.1:48378136-48378155 None:intergenic 35.0%
CTTATTGGAAGAATCTTAGG+TGG + chr4.1:48378134-48378153 MS.gene61579:CDS 35.0%
GAAGGTTTATTTGCTTTCTG+TGG + chr4.1:48378442-48378461 MS.gene61579:CDS 35.0%
GAATACAAAGACATGGTGAA+GGG + chr4.1:48378305-48378324 MS.gene61579:CDS 35.0%
TAGAAGGAAATTGTGGATGA+AGG - chr4.1:48378173-48378192 None:intergenic 35.0%
TCCTAAAAATCCTTGGTTGA+AGG + chr4.1:48378424-48378443 MS.gene61579:CDS 35.0%
TCTTAGTCCTAATGTGTAGA+AGG - chr4.1:48378189-48378208 None:intergenic 35.0%
TGCAAATCAACCTTCAAAGA+AGG - chr4.1:48378332-48378351 None:intergenic 35.0%
TGTTGGTATAACTTGAAGGA+TGG - chr4.1:48378285-48378304 None:intergenic 35.0%
TTAAGGGTCCTAAAAATCCT+TGG + chr4.1:48378417-48378436 MS.gene61579:CDS 35.0%
TTAGGATGGTTGTGATGAAT+AGG - chr4.1:48378088-48378107 None:intergenic 35.0%
TTCCAATAAGGCTTATTGTG+AGG - chr4.1:48378124-48378143 None:intergenic 35.0%
! ACCTTCAACCAAGGATTTTT+AGG - chr4.1:48378428-48378447 None:intergenic 35.0%
! ATCCTCACAATAAGCCTTAT+TGG + chr4.1:48378119-48378138 MS.gene61579:CDS 35.0%
! CTCTTCTATCTTTTGCCATA+AGG + chr4.1:48378219-48378238 MS.gene61579:CDS 35.0%
! TCTATTTATGTCACCGGTTA+AGG + chr4.1:48378637-48378656 MS.gene61579:CDS 35.0%
!! AGCCTTATTGGAAGAATCTT+AGG + chr4.1:48378131-48378150 MS.gene61579:CDS 35.0%
!! ATACAGGTGCTATTGTTGAA+GGG + chr4.1:48378351-48378370 MS.gene61579:CDS 35.0%
!! TTGAAGGTTGATTTGCATAC+AGG + chr4.1:48378335-48378354 MS.gene61579:CDS 35.0%
ATGAACCCTGCTAATGCATT+TGG + chr4.1:48378512-48378531 MS.gene61579:CDS 40.0%
CAAGCTTTCTTCTCCTTAAC+CGG - chr4.1:48378653-48378672 None:intergenic 40.0%
GGAGTGATTAAATCAGCCAT+AGG + chr4.1:48377957-48377976 MS.gene61579:CDS 40.0%
TTTGCTTTCTGTGGCAACTT+TGG + chr4.1:48378451-48378470 MS.gene61579:CDS 40.0%
!! CATACAGGTGCTATTGTTGA+AGG + chr4.1:48378350-48378369 MS.gene61579:CDS 40.0%
!! TACAGGTGCTATTGTTGAAG+GGG + chr4.1:48378352-48378371 MS.gene61579:CDS 40.0%
!! TTCTGTGGCAACTTTGGTTT+TGG + chr4.1:48378457-48378476 MS.gene61579:CDS 40.0%
AATAGGCCAGGTAGTGAAAC+TGG - chr4.1:48378071-48378090 None:intergenic 45.0%
ACCCTGCTAATGCATTTGGT+TGG + chr4.1:48378516-48378535 MS.gene61579:CDS 45.0%
AGGTTTCCTGCTCAAGCAAT+AGG + chr4.1:48378239-48378258 MS.gene61579:CDS 45.0%
ATGGTTGTGATGAATAGGCC+AGG - chr4.1:48378083-48378102 None:intergenic 45.0%
CAAGCAATAGGTGGAGCAAT+TGG + chr4.1:48378251-48378270 MS.gene61579:CDS 45.0%
CAGGTAGTGAAACTGGTTGA+AGG - chr4.1:48378064-48378083 None:intergenic 45.0%
CCCAACCAAATGCATTAGCA+GGG - chr4.1:48378520-48378539 None:intergenic 45.0%
CCCTGCTAATGCATTTGGTT+GGG + chr4.1:48378517-48378536 MS.gene61579:CDS 45.0%
CTTCAACCAGTTTCACTACC+TGG + chr4.1:48378062-48378081 MS.gene61579:CDS 45.0%
GCAGAACTAGCACCAATCAA+AGG - chr4.1:48378599-48378618 None:intergenic 45.0%
GCATTAGCAGGGTTCATAGA+AGG - chr4.1:48378509-48378528 None:intergenic 45.0%
GTGAAACTGGTTGAAGGTTG+AGG - chr4.1:48378058-48378077 None:intergenic 45.0%
TGCTTGAGCAGGAAACCTTA+TGG - chr4.1:48378237-48378256 None:intergenic 45.0%
TTTCCTGCTCAAGCAATAGG+TGG + chr4.1:48378242-48378261 MS.gene61579:CDS 45.0%
GCCCAACCAAATGCATTAGC+AGG - chr4.1:48378521-48378540 None:intergenic 50.0%
GGTGAAGGGTCCTTCTTTGA+AGG + chr4.1:48378319-48378338 MS.gene61579:CDS 50.0%
!! TGGTGCTAGTTCTGCTGCTA+TGG + chr4.1:48378604-48378623 MS.gene61579:CDS 50.0%
GCTCCACCTATTGCTTGAGC+AGG - chr4.1:48378248-48378267 None:intergenic 55.0%


Chromosome Type Strat End Strand Name
chr4.1 gene 48377954 48378673 48377954 ID=MS.gene61579
chr4.1 mRNA 48377954 48378673 48377954 ID=MS.gene61579.t1;Parent=MS.gene61579
chr4.1 exon 48377954 48378673 48377954 ID=MS.gene61579.t1.exon1;Parent=MS.gene61579.t1
chr4.1 CDS 48377954 48378673 48377954 ID=cds.MS.gene61579.t1;Parent=MS.gene61579.t1
Gene Sequence

>MS.gene61579

ATGGGAGTGATTAAATCAGCCATAGGAGATGCAATTTTAACATCAATATTTGTTTTCTTCATCTCAACATTAAGAATTCTCTCTACAAAAACATCTCTTTTCCTCAACCTTCAACCAGTTTCACTACCTGGCCTATTCATCACAACCATCCTAAACACAATCATTATCCTCACAATAAGCCTTATTGGAAGAATCTTAGGTGGTGCTAGCTTCAACCCTTCATCCACAATTTCCTTCTACACATTAGGACTAAGACCAGATTCATCTCTTCTATCTTTTGCCATAAGGTTTCCTGCTCAAGCAATAGGTGGAGCAATTGGCATCAAATCCATCCTTCAAGTTATACCAACAGAATACAAAGACATGGTGAAGGGTCCTTCTTTGAAGGTTGATTTGCATACAGGTGCTATTGTTGAAGGGGTTTTGACTTTTACTCATAACTTTGTTATTCTTCTTGTTATGCTTAAGGGTCCTAAAAATCCTTGGTTGAAGGTTTATTTGCTTTCTGTGGCAACTTTGGTTTTGGTTATTATTGGTTCTGGTTATACTGGACCTTCTATGAACCCTGCTAATGCATTTGGTTGGGCTTATATGAACAATAAGCACAATACTTGGGAGCAATTTTATGTTTTTTGGATATGTCCTTTGATTGGTGCTAGTTCTGCTGCTATGGTTTATCGATTTCTATTTATGTCACCGGTTAAGGAGAAGAAAGCTTGA

Protein sequence

>MS.gene61579.t1

MGVIKSAIGDAILTSIFVFFISTLRILSTKTSLFLNLQPVSLPGLFITTILNTIIILTISLIGRILGGASFNPSSTISFYTLGLRPDSSLLSFAIRFPAQAIGGAIGIKSILQVIPTEYKDMVKGPSLKVDLHTGAIVEGVLTFTHNFVILLVMLKGPKNPWLKVYLLSVATLVLVIIGSGYTGPSMNPANAFGWAYMNNKHNTWEQFYVFWICPLIGASSAAMVYRFLFMSPVKEKKA